CN116114721A - Method for producing biological herbicide by utilizing composite microorganism - Google Patents
Method for producing biological herbicide by utilizing composite microorganism Download PDFInfo
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- CN116114721A CN116114721A CN202310023777.9A CN202310023777A CN116114721A CN 116114721 A CN116114721 A CN 116114721A CN 202310023777 A CN202310023777 A CN 202310023777A CN 116114721 A CN116114721 A CN 116114721A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P13/00—Herbicides; Algicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for producing biological herbicide by utilizing composite microorganism, which belongs to the technical field of herbicide production, and the preparation method comprises the following steps: (1) determining the formulation of the biological herbicide; (2) preparing a composite microbial agent; (3) preparing a microbial active material; (4) preparing biological herbicide. The biological herbicide prepared by utilizing the synergistic effect of the microbial raw materials has good killing effect on various weeds, has quick response, no drug residue, can reduce the dosage of the medicament and the economic cost, is convenient to prepare, is environment-friendly, and has strong practical value.
Description
Technical Field
The invention relates to the field of herbicide production, in particular to a method for producing biological herbicide by utilizing compound microorganism.
Background
Eucalyptus is one of the famous three-speed high-yield forests in the world, has the characteristics of quick growth, strong adaptability, strong stress resistance and the like, and is widely introduced and cultivated in the world. Eucalyptus is introduced in 1890 in China, and main production areas are Guangdong, guangxi, hainan, yunnan, fujian and Sichuan. The eucalyptus has high utilization value and is widely applied to various industries such as papermaking, wood export, furniture equipment, medicines, foods, chemical industry, energy sources and the like, so the eucalyptus is a tree species with higher economic value. The eucalyptus forest is mostly positioned on hills or hills, weeds, mixed irrigation and various vines are easy to overgrow, the problem of damage of the eucalyptus forest by the mixed irrigation of the weeds is increasingly prominent, and the weeds not only strive for light, fertilizer and space (including underground space) with the eucalyptus forest; weeds also affect operations such as fertilization in young forest tending; the growth of weeds is high, and the weed is a main medium for transferring fire of forest fires in autumn and winter. Therefore, the eucalyptus forest needs to be continuously weeding to ensure high yield, and the cost of each forest land for weed control is greatly increased year by year.
In the past, people carry out operation on eucalyptus forest by using manpower or machinery, but because eucalyptus forest needs to be constructed in a wide area, the traditional weeding has the defects of labor and time consumption, high energy consumption and the like, and is gradually replaced by chemical weeding, however, the large-scale use of chemical agents also causes the problems of a series of herbicide resistant weed plants, long-term residue of soil organic pollutants, water quality pollution and the like. In recent years, with the enhancement of environmental awareness and the demand of sustainable high-quality development of forestry, the development of chemical pesticides faces a great pressure on the problem of environmental quality, and therefore, the development of biological herbicides or bionic herbicides with high biological activity, strong selectivity and good environmental compatibility has become an inevitable trend of the development of contemporary pesticides.
At present, most common sticktight, vine, fern, shrub and other weeds in eucalyptus forest, but the herbicides used in the market at present have single effect, the technical level of the herbicides is low, the period of using the herbicides is poor, the concentration ratio of the herbicides is unreasonable, the dosage is increased blindly, the herbicides with the same dosage form are used for many years in the same land, and the spraying tools lag behind and the like, so that the weeds in the eucalyptus forest generate drug resistance or drug resistance, some inferior weed seeds become dominant species, and the weed population evolves; the method has the advantages that some malignant weeds seriously occur, the whole quality is poor, the application effect of the herbicide to fern weeds, vine weeds, shrubs and other weeds in eucalyptus is reduced, and the sensitivity of the herbicide to the herbicide is also reduced year by year.
The microbial source herbicide is a novel biological herbicide for preventing and killing weeds by using metabolites produced by microorganisms. Herbicides of fungal, bacterial and streptomyces origin can be classified according to their origin, and mainly refer to toxins or antibiotic substances produced by microorganisms during metabolism. Known pathogenic toxins and antibiotics are organic compounds, including polypeptides, shadow masks, macrocyclic lipids, phenolic resins, and the like.
Dead bacillus cereus is a close relative to bacillus subtilis. The bacillus cereus can produce spores with strong resistance to heat, ultraviolet rays, electromagnetic jurisdiction and certain chemicals, can endure various bad environments, is beneficial to the production, processing and storage of the biocontrol microbial agent, and is an ideal biocontrol microorganism. The dead bacillus cereus competes with pathogenic bacteria for nutrition around plants by successfully colonizing to plant rhizosphere, body surface or body, secretes antibacterial substances to inhibit the growth of the pathogenic bacteria, and induces a plant defense system to resist the invasion of the pathogenic bacteria, so that the purpose of biocontrol is achieved. The bacillus subtilis preparation has the advantages of high growth speed, simple nutrition requirement, easy survival, colonization and reproduction on the surface of plants, simple process for producing the bacillus preparation, stable preparation, convenient application and long storage period, so that the dead bacillus cereus has good application prospect when used as a biocontrol microbial inoculum.
Pseudomonas aeruginosa is a recognized environment-friendly plant growth promoting bacterium, and the strain has wide distribution range and stronger adaptability to ecological environment. Most of the pseudomonas aeruginosa is obtained by separating from plant rhizosphere, and the metabolic products of the strain have certain protection effect on plants, have potential and application prospect of biocontrol, and overcome the defect of chemical pesticides at the same time, so that the strain becomes a real green pesticide, and therefore, the strain is widely focused in the field of biocontrol.
Chinese patent CN106900750a discloses a microbial herbicide, which comprises the following components in parts by weight: 1-3 parts of pseudomonas, 2-3 parts of sclerotinia, 3-7 parts of cercospora, 5-10 parts of glycine ester, 20-30 parts of ethanol, 2-5 parts of sodium dodecyl benzene sulfonate, 1-5 parts of metolachlor, 1-2 parts of polysilicone, 2-3 parts of polyoxyethylene sorbitan monooleate and 3-7 parts of an adsorption carrier. The microbial herbicide can balance the rapid mass growth of weeds according to the principle of ecology, is natural and environment-friendly, has little dosage, no toxic or side effect, reduces the damage to the environment, has no influence on the safety of crops, and can be used as the nutrition of crops to realize virtuous circle. However, the microbial herbicide only acts on weak weeds in farmlands, and does not have the problems of removing malignant weeds such as sticktight, vines, ferns, shrubs and the like which are relatively hard in vitality in eucalyptus forests, and possibly causing medicine residues, soil pollution and the like if chemical agents are added for preventing and controlling.
Based on the current situation, the invention utilizes the bacillus cereus, the pseudomonas aeruginosa and the fatty alcohol polyoxyethylene ether to prepare the biological herbicide which is friendly to the environment and can effectively remove malignant weeds in eucalyptus forests. At present, no report on the open literature for developing and preparing biological herbicide by utilizing the bacillus cereus, the pseudomonas aeruginosa and the fatty alcohol polyoxyethylene ether is found.
The foregoing background is only for the purpose of providing an understanding of the inventive concepts and technical aspects of the present invention and is not necessarily prior art to the present application and is not intended to be used to evaluate the novelty and creativity of the present application in the event that no clear evidence indicates that such is already disclosed at the filing date of the present application.
Disclosure of Invention
The invention aims to provide a method for producing biological herbicide by utilizing composite microorganism, which solves the technical problems that the weeding effect of pesticide on common vine, fern, shrub and other malignant weeds in eucalyptus forests is not ideal and the pesticide residue can pollute the environment during weeding in the prior art.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
a method for producing a biological herbicide using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 100-150 parts of composite microbial agent, 20-30 parts of microbial active material, 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa, and uniformly mixing according to a certain mass ratio;
(3) Preparing a microbial active material: firstly, weighing eucalyptus leaves, wheat bran and corn stalks with certain mass, crushing and mixing to obtain mixed powder raw materials, then, loading the mixed powder raw materials into a bioreactor, adding ethanol solution with the same mass as the mixed powder raw materials, stirring and soaking, then, placing in an oven for concentrating and drying, finally, cooling and crushing into powder with the particle size of 60-100 meshes;
(4) Preparation of biological herbicide: mixing 100-150 parts of the prepared composite microbial agent and 20-30 parts of microbial active material in a culture tank, adding 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent, mixing, regulating pH in the culture tank, and culturing at a certain temperature to make the concentration of fermentation liquor reach 10 x 10 8 -15*10 8 The biological herbicide can be obtained by the method of each mL.
Preferably, the surfactant is fatty alcohol polyoxyethylene ether.
Preferably, the auxiliary agent comprises one or more of a synergist and a stabilizer.
Preferably, the synergistic agent comprises one or a mixture of more of vegetable oil, peanut oil, rapeseed oil and soybean oil.
Preferably, the stabilizer comprises one or more of sucrose, cellulose and trehalose glycolipid.
Preferably, the volume ratio of dead bacillus cereus to pseudomonas aeruginosa in the step (2) is 1.5-2.5:2-4.
Preferably, the mass ratio of eucalyptus leaves, wheat bran and corn stalks in the step (3) is 2:1:1.
preferably, the concentration of the ethanol solution in step (3) is 50-60%.
Preferably, the soaking time in the step (3) is 10-12h, and the drying temperature is 60-70 ℃.
Preferably, in step (4), the pH is 5-7, the temperature is 25-35 ℃, and the incubation period is 2-5 days.
Compared with the prior art, the invention has the beneficial effects that:
(1) The biological herbicide prepared by utilizing the synergistic effect of the microbial raw materials has good killing effect on various weeds, has quick response, no drug residue, can reduce the dosage of the medicament and the economic cost, is convenient to prepare, is environment-friendly, and has strong practical value.
(2) According to the invention, through the mutual cooperation and synergistic effect of the bacillus cereus, the fatty alcohol polyoxyethylene ether and the pseudomonas aeruginosa, the bacillus cereus reduces the surface tension of the herbicide, so that the herbicide is endowed with high-efficiency permeability; the fatty alcohol polyoxyethylene ether has extremely high viscosity in a herbicide system, is beneficial to improving the adhesive force and the moisturizing performance of the liquid medicine, is more beneficial to the systemic absorption and the conductivity of bacillus cereus and pseudomonas aeruginosa, improves the dissolution effect and can promote the full play of the drug effect; the pseudomonas aeruginosa has an inhibiting effect, and the synergistic effect with bacillus cereus and fatty alcohol-polyoxyethylene ether enhances the penetration and wetting effects of the herbicide and further improves the weeding effect of the herbicide, so that the biological herbicide prepared by the invention can greatly improve the weeding efficiency, can also complete weeding indexes in a short period, and has the characteristics of long lasting period, convenient use, safety to trees, safety to people and environment, and the like.
Detailed Description
For a better understanding of the present invention, reference is made to the following examples, which are included within the scope of the present invention, but are not intended to limit the scope of the present invention.
A method for producing a biological herbicide using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 100-150 parts of composite microbial agent, 20-30 parts of microbial active material, 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa according to the proportion of 1.5-2.5:2-4, and uniformly mixing the materials;
(3) Preparing a microbial active material: firstly, weighing the materials with the mass ratio of 2:1:1, grinding and mixing eucalyptus leaves, wheat bran and corn stalks to obtain mixed powder raw materials, then loading the mixed powder raw materials into a bioreactor, adding 50-60% ethanol solution with the same mass as the mixed powder raw materials, stirring, soaking for 10-12h, then placing in a 60-70 ℃ oven for concentrating and drying, finally cooling and grinding into powder with the particle size of 60-100 meshes;
(4) Preparation of biological herbicide: mixing 100-150 parts of the prepared composite microbial agent and 20-30 parts of microbial active material in a culture tank, adding 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent, mixing, regulating pH value in the culture tank to 5-7, and culturing at 25-35deg.C for 2-5 days to obtain fermentation liquor with concentration of 10×10 8 -15*10 8 The biological herbicide can be obtained by the method of each mL.
The following is a description of more specific examples.
Example 1
A method for producing a biological herbicide using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 100 parts of composite microbial agent, 20 parts of microbial active material, 0.3 part of fatty alcohol polyoxyethylene and 2 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa according to the following ratio of 1.5:2, mixing uniformly in a volume ratio;
(3) Preparing a microbial active material: firstly, weighing the materials with the mass ratio of 2:1:1, grinding and mixing eucalyptus leaves, wheat bran and corn stalks to obtain mixed powder raw materials, then loading the mixed powder raw materials into a bioreactor, adding 50% ethanol solution with the same mass as the mixed powder raw materials, stirring and soaking for 10 hours, then placing the mixed powder raw materials into a 60 ℃ oven for concentrating and drying, and finally cooling and grinding the mixed powder raw materials into powder with the particle size of 60 meshes;
(4) Preparation of biological herbicide: mixing 100 parts of the prepared composite microbial agent and 20 parts of microbial active material in a culture tank, adding 0.3 part of fatty alcohol-polyoxyethylene and 2 parts of auxiliary agent, mixing, regulating the pH value in the culture tank to be 5, and culturing at 25 ℃ for 2 days to ensure that the concentration of fermentation liquor reaches 10 x 10 8 The biological herbicide can be obtained by the method of each mL.
Wherein, the auxiliary agent is synergist rapeseed oil and peanut oil with the volume ratio of 1:1 and a stabilizer cellulose.
Example 2
A method for producing a biological herbicide using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 130 parts of composite microbial agent, 25 parts of microbial active material, 0.5 part of fatty alcohol polyoxyethylene and 6 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa according to the following ratio of 2:3, mixing uniformly in a volume ratio;
(3) Preparing a microbial active material: firstly, weighing the materials with the mass ratio of 2:1:1, grinding and mixing eucalyptus leaves, wheat bran and corn stalks to obtain mixed powder raw materials, then loading the mixed powder raw materials into a bioreactor, adding 55% ethanol solution with the same mass as the mixed powder raw materials, stirring and soaking for 11 hours, then placing the mixed powder raw materials into a 65 ℃ oven for concentrating and drying, and finally cooling and grinding the mixed powder raw materials into powder with the particle size of 70 meshes;
(4) Preparation of biological herbicide: 130 parts of the prepared composite microbial agent and 25 parts of microbial active material are placed in a culture tank to be mixed, 0.5 part of fatty alcohol-polyoxyethylene and 6 parts of auxiliary agent are added to be mixed, the pH value in the culture tank is regulated to be 6, and the culture is carried out for 4 days at the temperature of 30 ℃ to ensure that the concentration of fermentation liquor reaches 13 x 10 8 The biological herbicide can be obtained by the method of each mL.
Wherein, the auxiliary agent is synergist rapeseed oil and peanut oil with the volume ratio of 1:1 and a stabilizer cellulose.
Example 3
A method for producing a biological herbicide using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 150 parts of composite microbial agent, 30 parts of microbial active material, 0.7 part of fatty alcohol polyoxyethylene and 8 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa according to 2.5:4, mixing uniformly in a volume ratio;
(3) Preparing a microbial active material: firstly, weighing the materials with the mass ratio of 2:1:1, grinding and mixing eucalyptus leaves, wheat bran and corn stalks to obtain mixed powder raw materials, then loading the mixed powder raw materials into a bioreactor, adding 60% ethanol solution with the same mass as the mixed powder raw materials, stirring and soaking for 12 hours, then placing the mixed powder raw materials into a 70 ℃ oven for concentrating and drying, and finally cooling and grinding the mixed powder raw materials into powder with the particle size of 100 meshes;
(4) Preparation of biological herbicide: mixing 150 parts of the prepared composite microbial agent and 30 parts of microbial active material in a culture tank, adding 0.7 part of fatty alcohol polyoxyethylene and 8 parts of auxiliary agent, mixing, regulating pH value in the culture tank to 7, and culturing at 35deg.C for 5 days to obtain the final productThe concentration of the fermentation liquor reaches 15 x 10 8 The biological herbicide can be obtained by the method of each mL.
Wherein, the auxiliary agent is synergist rapeseed oil and peanut oil with the volume ratio of 1:1 and a stabilizer cellulose.
Comparative example 1
The raw materials and the preparation method were substantially the same as in example 2, except that Bacillus cereus was not added to the raw materials for preparing the bioherbicide.
Comparative example 2
The raw materials and the preparation method were basically the same as in example 2, except that no fatty alcohol-polyoxyethylene ether was added to the raw materials for preparing the bioherbicide.
Comparative example 3
The starting materials and preparation methods were essentially the same as in example 2, except that no Pseudomonas aeruginosa was added to the starting materials for the preparation of the bioherbicide.
Comparative example 4
The raw materials and the preparation method are basically the same as those of example 2, except that dead bacillus cereus, fatty alcohol polyoxyethylene ether and pseudomonas aeruginosa are not added into the raw materials for preparing the biological herbicide.
7 eucalyptus forest weed lands with basically consistent weed types and growth conditions and the same area (10 m multiplied by 10 m) are selected, the biological herbicides prepared in the 3 examples are respectively applied to the 3 eucalyptus forest weed lands selected, the biological herbicides prepared in the comparative examples 1-4 are applied to the rest 4 eucalyptus forest weed lands as comparative examples, the change of weeds after application is recorded, and when the pesticide effect reaches the peak period, the weeding effect per unit area is investigated, and the results are shown in the following table:
TABLE 1 herbicidal effect on Eucalyptus forest lands of examples 1-3 and comparative examples 1-4
According to the data of the above embodiments in table 1, when the pesticide effect of the biological herbicide prepared by the invention on the forest land reaches the peak period, the weeding effect on the fern weeds reaches more than 97.84%, the vine weeds reach more than 91.75%, the shrub weeds reach more than 90.68%, and the weeding effect on the three weeds can reach more than 90%, wherein the weeding effect of the example 2 on the three weeds is the best, and is the best example of the invention. In addition, the data of examples 1-3 are all better than the data of comparative examples 1-4, demonstrating that the addition of bacillus cereus, fatty alcohol-polyoxyethylene ether and pseudomonas aeruginosa has the effect of improving the weeding effect of the herbicide.
Comparing the data of example 2 with comparative examples 1-4, example 2 was increased by 4.32%, 7.43%, 5.06%, 19.02% for fern weeds in woodland, respectively, compared to comparative examples 1, 2, 3, 4. Example 2 was 5.42%, 5.87%, 3.85%, 17.02% higher than comparative examples 1, 2, 3, 4, respectively, against vine weeds. Example 2 was 3.71%, 5.77%, 7.51%, 19.82% higher than comparative examples 1, 2, 3, 4, respectively, against shrub weeds. The weeding effect can be seen, and the biological herbicide prepared by the invention can effectively improve the weeding effect of fern weeds, vine weeds and shrub weeds; the average herbicidal effect of example 2 was higher than the average herbicidal effect enhancement rate of comparative examples 1 to 4, and it was also shown that the added Bacillus cereus, fatty alcohol-polyoxyethylene ether and Pseudomonas aeruginosa of the present invention played a role in synergistically enhancing the herbicidal effect of the herbicide.
According to the judgment, the weeding effect value generated when the dead bacillus cereus, the fatty alcohol polyoxyethylene ether and the pseudomonas aeruginosa are used together is improved by 13.14 percent more than 10 percent, 12.41 percent more than 10 percent and 16.66 percent more than 10 percent compared with the weeding effect value generated by superposition when the dead bacillus cereus, the fatty alcohol polyoxyethylene ether and the pseudomonas aeruginosa are respectively used independently, which indicates that the dead bacillus cereus, the fatty alcohol polyoxyethylene ether and the pseudomonas aeruginosa play a synergistic effect in preparing biological herbicide and synergistically improve the weeding effect.
This is because: the biological herbicide uses dead bacillus cereus and pseudomonas aeruginosa in removing eucalyptol weeds, and is combined with the surfactant fatty alcohol polyoxyethylene ether, so that the surface tension of the biological herbicide on the weeds is obviously improved, and the weeding effect of the herbicide is improved; the bacillus cereus can secrete antibacterial substances around the roots of the weed plants to inhibit the growth of pathogenic bacteria, and meanwhile, a plant defense system is induced to resist the invasion of the pathogenic bacteria, so that the purpose of inhibiting the growth of the weed plants is achieved from the roots; pseudomonas aeruginosa has selective inhibition on monocotyledonous She Caolei, is easy to generate infection structures such as attachment cells and the like after spores are contacted, and can rapidly diffuse and penetrate through leaves, and has stronger inhibition effect on plant diseases; however, as the dead bacillus cereus and the pseudomonas aeruginosa cannot attach to the surfaces of the eucalyptus forest malignant weeds for a long time to invade the plant bodies, the invention combines the surfactant fatty alcohol-polyoxyethylene ether to have stronger activity and viscosity, and synergistically promotes the dead bacillus cereus and the pseudomonas viridis attached to the surfaces of the weeds to spread to all wood tissues including roots, and can promote the full play of the drug effect when the drug liquid is transferred to each tissue, and the three cooperate to realize the accumulation of the infected plant in the wood due to the biological herbicide, thereby leading to the withering of the plant. The synergistic effect of the surfactant fatty alcohol polyoxyethylene ether, the dead bacillus and the pseudomonas aeruginosa can be mutually dissolved in a water solvent, so that the liquid medicine has higher viscosity, is favorable for improving the adhesive force and the moisturizing performance of the liquid medicine, reduces the surface tension of weeds, endows the biological herbicide with high-efficiency permeability, is more favorable for the systemic absorption and conductivity of the herbicide to weeds such as ferns, vines and shrubs in eucalyptus, can promote the full play of the pesticide effect, further improves the weeding effect of the herbicide, can finish weeding indexes in a short period, and has the characteristics of long duration, convenient use, no pesticide residue, basically harmless to eucalyptus, relatively safe to people and environment and the like.
The above description should not be taken as limiting the practice of the invention to these descriptions, but it will be understood by those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and the invention is defined by the appended claims.
Claims (10)
1. A method for producing a biological herbicide by using a composite microorganism, comprising the steps of:
(1) Determining the formulation of the biological herbicide: the formula comprises the following raw materials in parts by weight: 100-150 parts of composite microbial agent, 20-30 parts of microbial active material, 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent;
(2) Preparing a composite microbial agent: taking dead bacillus cereus and pseudomonas aeruginosa, and uniformly mixing according to a certain mass ratio;
(3) Preparing a microbial active material: firstly, weighing eucalyptus leaves, wheat bran and corn stalks with certain mass, crushing and mixing to obtain mixed powder raw materials, then, loading the mixed powder raw materials into a bioreactor, adding ethanol solution with the same mass as the mixed powder raw materials, stirring and soaking, then, placing in an oven for concentrating and drying, finally, cooling and crushing into powder with the particle size of 60-100 meshes;
(4) Preparation of biological herbicide: mixing 100-150 parts of the prepared composite microbial agent and 20-30 parts of microbial active material in a culture tank, adding 0.3-0.7 part of surfactant and 2-8 parts of auxiliary agent, mixing, regulating pH in the culture tank, and culturing at a certain temperature to make the concentration of fermentation liquor reach 10 x 10 8 -15*10 8 The biological herbicide can be obtained by the method of each mL.
2. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the surfactant is fatty alcohol polyoxyethylene ether.
3. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the auxiliary agent comprises one or more of a synergistic agent and a stabilizing agent.
4. A method for producing a bioherbicide using a composite microorganism as claimed in claim 3, wherein: the synergistic agent comprises one or a mixture of more of vegetable oil, peanut oil, rapeseed oil and soybean oil.
5. A method for producing a bioherbicide using a composite microorganism as claimed in claim 3, wherein: the stabilizer comprises one or more of sucrose, cellulose and alginic acid.
6. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: in the step (2), the volume ratio of the dead bacillus cereus to the pseudomonas aeruginosa is 1.5-2.5:2-4.
7. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the mass ratio of the eucalyptus leaves, the wheat bran and the corn stalks in the step (3) is 2:1:1.
8. a method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the concentration of the ethanol solution in the step (3) is 50-60%.
9. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the soaking time in the step (3) is 10-12h, and the drying temperature is 60-70 ℃.
10. A method for producing a bioherbicide using a composite microorganism as claimed in claim 1, wherein: the pH value in the step (4) is 5-7, the temperature is 25-35 ℃, and the culture time is 2-5 days.
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