CN106417393A - Preparation method of environmental-friendly composite herbicide - Google Patents
Preparation method of environmental-friendly composite herbicide Download PDFInfo
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- CN106417393A CN106417393A CN201610836339.4A CN201610836339A CN106417393A CN 106417393 A CN106417393 A CN 106417393A CN 201610836339 A CN201610836339 A CN 201610836339A CN 106417393 A CN106417393 A CN 106417393A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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Abstract
The invention relates to a preparation method of an environmental-friendly composite herbicide. The preparation method includes the following steps: firstly preparing a plant extract and a microbial agent; mixing diatomite, plant ash, carboxymethylcellulose, alkylbenzenesulfonate, polyvinyl alcohol, fatty alcohol-polyoxyethylene ether and propylene glycol uniformly; adding water in the mixture, and stirring at a rotation speed of 1200rpm for 30 min to obtain a suspension; stirring the suspension at a rotation speed of 800 rpm, and slowly adding the plant extract and the microbial agent in the suspension; after adding the plant extract and the microbial agent, continuing stirring for 10 min to obtain the composite herbicide. The prepared herbicide has good effects on the removal of weeds and autotrophic wheat seedlings, greatly increases the yield of rice and does not bring about the problems such as chemical residues. The herbicide is environment-friendly and has no pollution, and the application prospect is broad.
Description
Technical field
The present invention relates to biological pesticide and preparation method thereof is and in particular to a kind of preparation method of environmental protection compound herbicide.
Background technology
Herbicide, main pass through suppression, hinder or interference plant various important physiological and biochemical procedure, make the weeds can not be just
Be frequently grown growth and withered.In recent years, various herbicides use in a large number in China, and the use of herbicide reduces for peasant
Cost, reduces labour force, improves the productivity, be the performance of modern agricultural production progress.But because blindness is abused, in a large number
Or all kinds of herbicide of Use out of range, not only have influence on agricultural production and crop yield, and bring seriously to ecological environment
Negative effect, life-time service such as glyphosate-class herbicides, make weeds produce drug resistance, existing plant source biological weed killer sends out
Wave effect slow, weeding is not thorough, and major part still has the interpolation of the conventional medicaments such as glyphosate, be good for the body of ecological environment and people
Health brings harm.Sulfonylurea herbicide preparation is class herbicidal formulations of usage amount maximum in the world at present, and this herbicide is the most prominent
The problem going out is the long residual drug injury problem to succession crop, because its migration leaching is weaker, when its residual reaches certain agent
Poisoning will be caused to succession crop after amount.It is residual that the field of some life-time service long residual effect herbicidal formulations have also appeared herbicidal formulations
The situation of allowance accumulation, has a strong impact on the growth of succession crop, defines the phenomenon in " cancer field ".The harmful substance of its residual
Drastically influence the healthy of the mankind.From the viewpoint of long-range, a large amount of of herbicidal formulations use caused residual hazard poisoning, very
Extremely crops Severe Reduction can be led to.
However, in recent years, regional in part rice wheat continuous cropping, particularly as Hubei, Zhejiang, Jiangsu etc. save, more and more
The local rice direct seeding field of reaction, spy is standby to be that the generation of dry direct-seeding rice Tanaka's volunteer wheat Seedling is very serious, or even has become as rice field danger
The heaviest weeds being most difficult to prevent and treat of evil.After generally little wheat harvesting, if with large-scale harvester, the wheat seeds in unrestrained field have 80 jin with
On；If using small harvester, unrestrained wheat seeds have 10-20 jin, and these wheat seeds will certainly bear wheat seedling impact Oryza sativa L. and produce receipts.Typically build
Water on view rice field, and water conservation 2～3 days, can be drowned most spontaneous wheat seedling, but for dry seeded rice field, this is typically very
Difficult realization.Also some advises high temperature, and spontaneous wheat seedling i.e. can natural death.But in fact the fact that field it is a lot of places
" spontaneous wheat seedling " cannot be prevented and kill off, and always with paddy growth to results, but Semen Tritici aestivi is entirely flat, shaky, rice yield
Therefore can significantly decrease.After rice seedlings early application clomazone or metamifop spontaneous wheat seedling growth is had stronger
Inhibitory action, although these preparation herbicidal effects are notable, easily poisoning caused to rice seedling, and succession crop also can be produced
Raw significantly impact.And be used alone a kind of herbicide and have that weed control spectrum is narrow, integrated control effect is undesirable, dosage is higher,
Weeds are easier to the problems such as produce resistance.
Microbial herbicide therefore arises at the historic moment, and using the microorganism management of weeds history of existing nearly 200 years, only has in the past
Using natural enemies such as phytophagous animal, pathogenic microorganisms in its natural state, by Ecological path, weed population is controlled in warp
With aesthetic acceptable level in Ji, in ecology.With people, phytopathogen understanding is goed deep into, the middle of last century opens
Begin the developmental research of microbial herbicide.Because host weeds separate the phytopathogen obtaining, host plant is typically had
Inter-species specificity, selectivity is high, so safe to cultivated plant, harm is little.The non-target organisms such as people, animal, natural enemy are pacified
Entirely, the contradiction of biological and ecological methods to prevent plant disease, pests, and erosion and chemoprevention can be alleviated, and relatively small to ambient pressure.Raising with global environment consciousness and agricultural
The needs of sustainable development, efficiently, environmental protection, the research of harmless microbial herbicide increasingly show its important social meaning
And economic worth.
But existing microbial herbicide still has a lot of problems, thus limiting sending out of Modern microbiological herbicide
Exhibition.The principal element of developmental limitation has：By control weeds enrich genetic diversity, the high specificity of biological weed killer, its
The environmental conditions such as temperature, humidity and soil are bordering on the demand of harshness, not the matching of Industrialized processing technique and equipment, formula grinds
Study carefully backwardness of technology etc..
Currently in order to improving the efficiency of herbicidal formulations, people are generally compounded using multiple bacterial strains, and compounding use is not only
The advantage that chemical agent bactericidal speed is fast, prevention effect is stable can be played, also there is holding Biocontrol microorganism simultaneously and exist
Colonizing around plant roots, leaf and the features such as secrete antibiotic.How to be compounded from suitable bacterial strain, and reached excellent
Gesture is complementary, consumption reduces, the enhanced purpose of preventive effect, is the problem that compounding herbicidal formulations need to solve.
Content of the invention
It is an object of the invention to overcoming the deficiencies in the prior art and providing a kind of environmental protection compound herbicide and its application.
For realizing the object of the invention, the application adopts the following technical scheme that：
A kind of preparation method of environmental protection compound herbicide, described herbicide, it is by microbial bacterial agent, plant extract, auxiliary agent group
Become.
Preferably, described environmental protection compound herbicide, it is by the raw material preparation of following weight portion：
Microbial bacterial agent 10-12 part, plant extract 20-25 part, kieselguhr 6-8 part, plant ash 7-10 part, carboxymethyl cellulose
5-6 part, alkylbenzenesulfonate 3-4 part, polyvinyl alcohol 2-3 part, fatty alcohol-polyoxyethylene ether 2-3 part, propylene glycol 1-2 part, water 50-
70 parts.
Described plant extract is by Herba Xanthii, Herba Pteridis Multifidae, Radix Cirsii Japonici, Folium Perillae, Herba Plantaginiss alcohol extraction preparation.
The preparation method of described plant extract is as follows：Take 20 parts of Herba Xanthii, 20 parts of Herba Pteridis Multifidae, 18 parts of Radix Cirsii Japonici, 15 parts of Folium Perillae,
10 parts of crushed after being dried of Herba Plantaginiss and mix acquisition plant powder standby；
Add 95% ethanol of 3 times of weight of plant powder（Volume fraction）, after extraction 1-3d, filter and obtain extracting solution, by extracting solution
Concentrating under reduced pressure, to no longer there being solvent to flow out, obtains concentrated extract；Take 0.5g concentrated extract, add 0.5ml triethanolamine,
Again plus distilled water is settled to 100ml, stirred with Glass rod；Then ultrasonic activation 10min, so as to abundant dissolve, obtains plant
Extract.
Described microbial bacterial agent includes Lilaceous streptomycete, curvularia lunata bacterium, candidiasis, narrow ovum alternaric bacteria system
Standby；
The preparation method of described microbial bacterial agent is：By Lilaceous streptomycete, curvularia lunata bacterium, candidiasis, narrow ovum chain
It is 1 × 10 that lattice spore bacterium is cultivated respectively to concentration6The bacterium solution of individual/ml, then according to 3:3:5:4 volume ratio mixing, obtains final product；
Described Lilaceous streptomycete（Streptomyces lavendulae）For CGMCC NO.1368（CN1928068）；
Described curvularia lunata bacterium（Curvularia Lunata）For CGMCC NO.2928 (CN101812407);
Described candidiasis are（Candida utilis）ATCC No.22023;
Narrow ovum rod method bacterial strain is Alternariaaugustiovoidea strains A AEC05-3（Referring to narrow ovum rod method bacterial strain
AAEC05-3 and its toxin are pathogenic to barnyard grass, plant protection journal, and 2007）
The preparation method of described environmental protection compound herbicide is：
（1）Prepared plant extracts；
（2）Prepare microbial bacterial agent；
（3）By kieselguhr, plant ash, carboxymethyl cellulose, alkylbenzenesulfonate, polyvinyl alcohol, fatty alcohol-polyoxyethylene ether, third
Glycol mix homogeneously, adds water, 1200 revs/min of rotating speed stirs 30 minutes, obtains suspension；
（4）In step（3）The suspension of preparation is stirred, is slowly added microbial bacterial agent, plant with 800 revs/min of rotating speeds
Thing extract, continues stirring and obtains final product for 10 minutes after all having added.
The having the beneficial effect that of hinge structure of the present invention：
From phytochemicalss, exploitation natural herbicide can reduce the impact that environment, human body health are caused, simultaneously
Reduce the Drug resistance to herbicide for the weed population, plant is the natural treasure-house of bioactive compound.Evolutionary process in plant
In, usually produce some compounds to suppress the growth of other plant, this phenomenon is referred to as allelopathy, i.e. various plants
（Including higher plant and microorganism）Biochemical interpromoting relation in five elements and antgonistic action that the chemical substance being discharged causes, also referred to as different strain gram
Raw or restriction or checking relation in five elements interpromoting relation in five elements, the chemical substance that it produces is referred to as allelochemical, using plant between allelopathy control
Weeds processed find that from plant herbicide lead compound is an important channel of new herbicides exploitation, with natural product
For guide's chemical combination
Thing carries out the important directions that herbicide development is herbicide exploitation, and the present invention adopts single-factor and multiple-factor optimization Test true
Recognize Herba Xanthii, Herba Pteridis Multifidae, Radix Cirsii Japonici, Folium Perillae, Herba Plantaginiss combine according to a certain ratio can obtain optimum rice field remove wheat seedling and its
His weeds effect.
The host speciality of microorganism herbicidal formulations is its advantage, is also its major defect simultaneously.Single micro-
Biological weed control preparation to the specialization characteristic of weed control so as to be difficult to farmland weed is effectively prevented and kill off, thus just limiting
Microorganism herbicidal formulations popularization and application.Some researchs show, go out complex microorganism weeding using multiple biocontrol bacterial strain mixed developings
Preparation is the effective way solving the excessively single defect of the anti-weeds of microorganism herbicidal formulations, the study show that, pale purple using bacterial strain
Compounding between color streptomycete, curvularia lunata bacterium, candidiasis, narrow ovum alternaric bacteria can preferably prevent and kill off volunteer wheat
Seedling, Semen Euphorbiae, barnyard grass, Amaranthus retroflexus etc., provide good reference for carrying out biocontrol microorganisms applied research from now on.
Herbicidal formulations of the present invention do not contain the chemical weed control preparation such as sulfonylurea, amide-type, it is to avoid herbicidal formulations residuals
Amount accumulation protects soil and environment, makes with auxiliary combinations such as specific surfactant, synergist, dispersant, wetting agent
Microorganism herbicidal formulations have strong adaptability, good stability, non-aging the features such as strain, and rationally utilize microorganism between
Synergism, not only good and nontoxic to the action effect of weeds, ecological, environmental protective, significantly reduce dosage, dispenser time
Number and drug cost, and this herbicidal formulations is prepared conveniently, the holding time is long, has very strong practical value.
Specific embodiment
Embodiment 1
A kind of preparation method of compound herbicide, described herbicide is prepared by the raw material of following weight portion：
10 parts of microbial bacterial agent, 20 parts of plant extract, 6 parts of kieselguhr, 7 parts of plant ash, 5 parts of carboxymethyl cellulose, alkylbenzene
3 parts of sulfonate, 2 parts of polyvinyl alcohol, 2 parts of fatty alcohol-polyoxyethylene ether, 1 part of propylene glycol, 50 parts of water.
The preparation method of described plant extract is as follows：Take 20 parts of Herba Xanthii, 20 parts of Herba Pteridis Multifidae, 18 parts of Radix Cirsii Japonici, 15 parts of Folium Perillae,
10 parts of crushed after being dried of Herba Plantaginiss and mix acquisition plant powder standby；
Add 95% ethanol of 3 times of weight of plant powder（Volume fraction）, after extraction 1-3d, filter and obtain extracting solution, by extracting solution
Concentrating under reduced pressure, to no longer there being solvent to flow out, obtains concentrated extract；Take 0.5g concentrated extract, add 0.5ml triethanolamine,
Again plus distilled water is settled to 100ml, stirred with Glass rod；Then ultrasonic activation 10min, so as to abundant dissolve, obtains plant
Extract.
The preparation method of described microbial bacterial agent is：By Lilaceous streptomycete, curvularia lunata bacterium, candidiasis, narrow
It is 1 × 10 that ovum alternaric bacteria is cultivated respectively to concentration6The bacterium solution of individual/ml, then according to 3:3:5:4 volume ratio mixing, obtains final product；
Described Lilaceous streptomycete（Streptomyces lavendulae）For CGMCC NO.1368；
Described curvularia lunata bacterium（Curvularia Lunata）For CGMCC NO.2928;
Described candidiasis are（Candida utilis）ATCC No.22023;
Narrow ovum rod method bacterial strain is Alternariaaugustiovoidea strains A AEC05-3（Referring to narrow ovum rod method bacterial strain
AAEC05-3 and its toxin are pathogenic to barnyard grass, plant protection journal, and 2007）
The preparation method of described environmental protection compound herbicide is：
（1）Prepared plant extracts；
（2）Prepare microbial bacterial agent；
（3）By kieselguhr, plant ash, alkylbenzenesulfonate, polyvinyl alcohol, fatty alcohol-polyoxyethylene ether, mixed with propylene glycol uniformly,
Add water, 1200 revs/min of rotating speed stirs 30 minutes, obtains suspension；
（4）In step（3）The suspension of preparation is stirred, is slowly added microbial bacterial agent, plant with 800 revs/min of rotating speeds
Thing extract, continues stirring and obtains final product for 10 minutes after all having added.
Herbicidal formulations amount of application of the present invention is 5ml/m2Apply.
Embodiment 2
Using greenhouse pot culture method, plant process is carried out to each weeds target, using front stirring, each processes in triplicate,
Separately set blank.Sprayer unit is POTTER type auto spraying tower, 30 microns of fogdrop diameter, effective adhesive rate ＞ 99%.Place
Be placed in greenhouse growth after reason, plant reaction symptom is observed in timing, and after medicine 10,20 days according to 0-100% staging
Visual assessment synthesis activity of weeding, evaluation criterion is shown in Table 1.
Experimental group：Spray the environmental protection compound herbicide of embodiment 1 preparation；
Compare 1 group：Spray the plant extract of embodiment 1 preparation, auxiliary agent adjusts in right amount；
Compare 2 groups：Spray the microorganism formulation of embodiment 1 preparation, auxiliary agent adjusts in right amount；
Above each group, the amount of spraying is all with 5ml/m2Apply.
Table 1 evaluation criterion
| Activity of weeding (%) | Activity of weeding synthesis comment (to plants stems or root suppression, deformity, albefaction, the influence degree such as rotten) |
| 0 | With compareing, inactive |
| 10-30 | Activity is low, slightly affects |
| 40-50 | Activity is low, and growth is had a significant effect |
| 60-70 | Active, can Developing restraint |
| 80-90 | Active good, serious Developing restraint |
| 100 | Plant is dead |
After spraying 10 days, result of the test is as shown in table 2：
Table 2 sprays 10 days
| Species | Spontaneous wheat seedling | Barnyard grass | Herba Digitariae | Semen Euphorbiae | Draw rattan | Amaranthus retroflexus |
| 1 group of embodiment | 91 | 91 | 89 | 88 | 95 | 90 |
| Compare 1 group | 53 | 67 | 45 | 58 | 70 | 66 |
| Compare 2 groups | 68 | 63 | 60 | 55 | 62 | 67 |
After spraying 20 days, result of the test is as shown in table 3：
Table 3 sprays 20 days
| Species | Spontaneous wheat seedling | Barnyard grass | Herba Digitariae | Semen Euphorbiae | Draw rattan | Amaranthus retroflexus |
| 1 group of embodiment | 98 | 99 | 97 | 94 | 97 | 98 |
| Compare 1 group | 60 | 69 | 51 | 60 | 71 | 70 |
| Compare 2 groups | 71 | 65 | 64 | 59 | 68 | 72 |
Embodiment 3
Experimental site selects Zhejiang Province's rice terrace, experimental field for typical paddy and wheat rotation plough land, middle fertility.Soil types is sand
Loam, pH value is 7.1, the content of organic matter 2.3%.Carry out field management by Direct-seeding Rice method.Before this field, stubble is Semen Tritici aestivi, Oryza sativa L.
Unused herbicide after broadcasting, weeds are mainly spontaneous wheat seedling, barnyard grass, Herba Digitariae, Semen Euphorbiae, draw rattan, Amaranthus retroflexus etc..
Experimental group：The environmental protection compound herbicide of Example 1 preparation, herbicide application amount is 5ml/m2Apply；
Blank control group：Do not apply any herbicide；
Chemical herbicide matched group：10% metamifop cream；
Set 3 repetitions altogether.
Result：Safety：20 days after dispenser, Oryza sativa L. growing way, plant height, leaf color are consistent with the Oryza sativa L. of blank district, no appoint
What poisoning phenomenon occurs.
Prevention effect：
Dispenser investigated Weed-control effect after 30 days, was counted according to weeds species, for spontaneous wheat seedling, barnyard grass, Herba Digitariae, a thousand pieces of gold
Son, draw the weeding rate of rattan, Amaranthus retroflexus to be respectively：98%、99%、97%、97%、98%、99%.
And chemical herbicide matched group to spontaneous wheat seedling, barnyard grass, Herba Digitariae, Semen Euphorbiae, draw the weeding rate of rattan, Amaranthus retroflexus to divide
It is not：65%、70%、82%、83%、79%、78%.
Per mu yield：
Blank control group is：527kg/ mu, chemical herbicide matched group is：594 kg/ mus, and experimental group yield is 647kg/
Mu.From the foregoing, it will be observed that environmental protection compound herbicide of the present invention is cut weeds and spontaneous wheat seedling effect is good, greatly improve rice yield, and not
The problems such as chemical residual can be brought, environment friendly and pollution-free, have a extensive future.
Although, above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications made without departing from theon the basis of the spirit of the present invention, the expansion of improvement or scope, belong to application claims
The scope of protection.
Claims (8)
1. a kind of preparation method of environmental protection compound herbicide is it is characterised in that described herbicide is by plant extract, microbial bacteria
Agent and auxiliary agent composition.
2. preparation method according to claim 1 is it is characterised in that comprise the steps：
（1）Prepared plant extracts；
（2）Prepare microbial bacterial agent；
（3）By kieselguhr, plant ash, carboxymethyl cellulose, alkylbenzenesulfonate, polyvinyl alcohol, fatty alcohol-polyoxyethylene ether with
And mixed with propylene glycol is uniformly, add water, 1200 revs/min of rotating speed stirs 30 minutes, obtains suspension；
（4）In step（3）The suspension of preparation is stirred, is slowly added microbial bacterial agent, plant with 800 revs/min of rotating speeds
Thing extract, continues stirring and obtains final product for 10 minutes after all having added.
3. the preparation method according to claim 1-2 it is characterised in that
Described plant extract is prepared by Herba Xanthii, Herba Pteridis Multifidae, Radix Cirsii Japonici, Folium Perillae and Herba Plantaginiss alcohol extraction.
4. the preparation method according to claim 1-3 is it is characterised in that the preparation method of described plant extract is as follows：
Take 20 parts of Herba Xanthii, 20 parts of Herba Pteridis Multifidae, 18 parts of Radix Cirsii Japonici, 15 parts of Folium Perillae, 10 parts of Herba Plantaginiss according to weight portion, crushed after being dried simultaneously mixes
Obtain plant powder standby；Then add 95% ethanol of 3 times of weight of plant powder（Volume fraction）, after extraction 1-3d, filtration obtains
Obtain extracting solution, extracting solution concentrating under reduced pressure obtains concentrated extract；Take 0.5g concentrated extract, add 0.5ml triethanolamine,
Again plus distilled water is settled to 100ml, stirred with Glass rod；Then ultrasonic Treatment 10min, so as to abundant dissolve, obtains plant
Extract.
5. the preparation method according to claim 1-4 it is characterised in that described herbicide by following weight parts raw material system
Standby：Microbial bacterial agent 10-12 part, plant extract 20-25 part, kieselguhr 6-8 part, plant ash 7-10 part, carboxymethyl cellulose
5-6 part, alkylbenzenesulfonate 3-4 part, polyvinyl alcohol 2-3 part, fatty alcohol-polyoxyethylene ether 2-3 part, propylene glycol 1-2 part, water 50-
70 parts.
6. the preparation method according to claim 1-5 is it is characterised in that described microbial bacterial agent includes lavender strepto-
Bacterium, curvularia lunata bacterium, candidiasis, narrow ovum alternaric bacteria.
7. the preparation method according to claim 1-6 is it is characterised in that the preparation method of described microbial bacterial agent is：Will
It is 1 × 10 that Lilaceous streptomycete, curvularia lunata bacterium, candidiasis, narrow ovum alternaric bacteria are cultivated respectively to concentration6Individual/ml
Bacterium solution, then according to 3:3:5:4 volume ratio mixing, obtains final product.
8. the weeding purpose of the preparation method described in claim 1-8.
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