CN109486702A - A kind of composite bacteria agent and preparation method thereof enhancing salt tolerance of wheat - Google Patents
A kind of composite bacteria agent and preparation method thereof enhancing salt tolerance of wheat Download PDFInfo
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- CN109486702A CN109486702A CN201811237534.0A CN201811237534A CN109486702A CN 109486702 A CN109486702 A CN 109486702A CN 201811237534 A CN201811237534 A CN 201811237534A CN 109486702 A CN109486702 A CN 109486702A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention belongs to microorganism field, a kind of composite bacteria agent and preparation method thereof enhancing salt tolerance of wheat.The complex micro organism fungicide and preparation method thereof of a kind of enhancing salt tolerance of wheat of the invention, which is characterized in that the microbial composite bacteria includes the strain of following mass parts: 50-80 parts of azospirillum, 45-70 parts of pseudomonad, 20-40 parts of bacillus, 15-30 parts of streptomycete, 20-35 parts of yeast.Complex micro organism fungicide of the invention can improve the salt stress that plant is subject to, and can not only enhance the salt tolerance of wheat, moreover it is possible to improve soil property, enhance soil fertility, promote absorption of the wheat to nutriment, improves wheat yield, increase farmers' income, a kind of method for the problem of being environmentally friendly effective solution salt-soda soil land resource waste, meanwhile the complex micro organism fungicide handled by Freeze Drying Technique, it is readily transported, transportation cost is saved, there is good popularization and application foreground.
Description
Technical field
The invention belongs to microorganism fields, and in particular to it is a kind of enhance salt tolerance of wheat composite bacteria agent and its preparation side
Method.
Background technique
With the growth of population and the development of social economy, cultivated area is gradually reduced, and land quality is also declining, the world
It is increasingly severe the problem of saline Land in range.Excessive soluble-salt will affect crop water suction, cause crop water suction tired
Difficulty causes burn seedlings.In addition, working as the excessively high absorption that will affect other ions of certain ion concentration, and then influence the suction of crop nutrition content
It receives.Meanwhile certain ion pair crops have direct toxic action, if chloride ion may cause to burn to crop, so that plant
It is difficult to survive on alkaline land soil.Although salt-soda soil is a kind of available resource, since alkaline land soil fertility is low, reason
Change character is poor, and more to the harmful anions and canons of crop, crop yield is low, and economic benefit is low, thus the land resource is still a large amount of
It is wasted.Select suitable microbial inoculum make plant salt tolerance alkali ability enhance, planted on salt-soda soil, can improve soil and
The problem of can increase farmers' income, being a kind of environmentally friendly effective solution salt-soda soil land resource waste.
Summary of the invention
The present invention is in view of the above-mentioned problems, provide a kind of composite bacteria agent for enhancing salt tolerance of wheat, which can
The ability for enhancing the saline-alkali tolerant of wheat, in addition, the composite bacteria agent can increase suction of the wheat to magnesium ion, potassium ion, calcium ion
It receives, reduces the absorption of sodium ion, improve the generation of heteroauxin, promote the growth of wheat, improve the yield of wheat, and cost
Cheap, application is easy to operate, pollution-free, is a kind of fertilizer of green high-efficient, has good popularization and application foreground.
A kind of composite bacteria agent of enhancing salt tolerance of wheat of the invention, the microbial composite bacteria include following mass parts
Strain: 50-80 parts of azospirillum, 45-70 parts of pseudomonad, 20-40 parts of bacillus, 15-30 parts of streptomycete, yeast 20-35
Part.
Preferably, a kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the microbial composite bacteria
Strain including following mass parts: 60-70 parts of azospirillum, 50-65 parts of pseudomonad, 30-38 parts of bacillus, actinomyces
18-25 parts, 25-33 parts of yeast.
Preferably, a kind of complex micro organism fungicide for enhancing salt tolerance of wheat, the microbial composite bacteria includes following
The strain of mass parts: 65 parts of azospirillum, 63 parts of pseudomonad, 33 parts of bacillus, 21 parts of actinomyces, 29 parts of yeast.
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the azospirillum is by azospirillum brasilense spiral shell
Bacterium produces one or more of rouge azospirillum bacterial strain composition.
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the bacillus is by Su Yunjin gemma
The bacterial strain of one or more of bacillus, bacillus subtilis, bacillus licheniformis, Paenibacillus polymyxa forms.
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the streptomycete is by giving birth to black spore strepto-
The bacterial strain composition of one or more of bacterium, avermectin streptomycete, Streptomycesalbidoflhaving, grey streptomyces verticillus.
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the yeast is by Candida, crisp wall
The bacterial strain of one or more of kluyveromyces forms.
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, comprising the following steps:
(1) various purified strains are taken out from the inclined-plane of preservation, is inoculated into prepares and sterilized respectively
Culture medium in, the inoculum concentration of each strain is 3% by mass percentage, be placed under conditions of 30 DEG C, 180rpm/min vibration
Swing culture 36-48h；
(2) cultured bacterial strain is diluted to 5 × 109A/ml, by 5 × 109Bacterium solution is adsorbed to carrier by the ratio of a/g
On, it is mixed later in the strain ratio of each microbial inoculum, obtains complex micro organism fungicide；
(3) the mixed bacteria culture solution that preparation is completed is centrifuged under conditions of 4000r/min 15min, collects precipitating, then
Drying will be precipitated, obtains Mixed Microbes powder.
Compared with prior art, beneficial effects of the present invention are as follows:
(1) present invention uses azospirillum for microbial inoculum main component, hence it is evident that reduce salt stress to the adverse effect of wheat,
Wheatear height, fringe weight, tiller number, plant gross dry weight have significant increase, and microbial inoculum of the invention not only enhances the resistance to of wheat
Salt, moreover it is possible to promote the growth of wheat, improve yield and wheat quality；
(2) present invention enhances absorption of the wheat to potassium, calcium and magnesium ion using pseudomonad as raw material, improves heteroauxin
Generation, reduce the content of abscisic acid, promote cell growth；
(3) present invention can prevent fertilizer point and water loss using bacillus, streptomycete, yeast as raw material, and can
The macromolecular substances that hardly possible is decomposed are resolved into available small-molecule substance by the amylase for releasing high activity；It generates abundant
It is metabolized product, synthesizes the substances such as a variety of organic acids, enzyme, physiological activity, enhances soil fertility.
In conclusion complex micro organism fungicide of the invention can improve the salt stress that plant is subject to, can not only enhance small
The salt tolerance of wheat, moreover it is possible to improve soil property, enhance soil fertility, promote absorption of the wheat to nutriment, improve wheat and produce
A kind of method for the problem of amount, increases farmers' income, and is environmentally friendly effective solution salt-soda soil land resource waste, meanwhile, pass through
The complex micro organism fungicide that Freeze Drying Technique is handled, is readily transported, and saves transportation cost, has good popularization and application
Prospect.
Specific embodiment
For a better understanding of the present invention, below with specific example come the technical solution that the present invention will be described in detail.
Embodiment 1
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the strain including following mass parts: fixed nitrogen
50 parts of spirillum, 70 parts of pseudomonad, 20 parts of bacillus, 15 parts of streptomycete, 35 parts of yeast.
The azospirillum is made of Azospirillum brasilense, and the bacillus is made of bacillus thuringiensis,
The streptomycete is made of grey streptomyces verticillus, and the yeast is made of Candida.
Compared with control group experimental result, after applying the complex micro organism fungicide in this example, after wheat cultivation 170d, often
The grain weight of strain wheat increases 3.4 times compared with control group, and the tiller number of every plant of wheat plant increases 1.8 times compared with control group,
The seed number of every plant of wheat plant improves 4.1 times compared with control group.
Embodiment 2
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the strain including following mass parts: fixed nitrogen
80 parts of spirillum, 45 parts of pseudomonad, 40 parts of bacillus, 30 parts of streptomycete, 20 parts of yeast.
The azospirillum is made of production rouge azospirillum, and the bacillus is made of bacillus licheniformis, institute
The streptomycete stated is formed by giving birth to melanosporin, and the yeast is made of Candida.
Compared with control group experimental result, after applying the complex micro organism fungicide in this example, after wheat cultivation 170d, often
The grain weight of strain wheat increases 3.3 times compared with control group, and the tiller number of every plant of wheat plant increases 1.9 times compared with control group,
The seed number of every plant of wheat plant improves 3.9 times compared with control group.
Embodiment 3
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the microbial composite bacteria include following matter
The strain of amount part: 60 parts of azospirillum, 50 parts of pseudomonad, 38 parts of bacillus, 18 parts of actinomyces, 33 parts of yeast.
The azospirillum is made of Azospirillum brasilense, and the bacillus is made of bacillus subtilis, institute
The streptomycete stated is made of avermectin streptomycete, the yeast Kluyveromyces fragilis composition.
Compared with control group experimental result, after applying the complex micro organism fungicide in this example, after wheat cultivation 170d, often
The grain weight of strain wheat increases 4.1 times compared with control group, and the tiller number of every plant of wheat plant increases 2.3 times compared with control group,
The seed number of every plant of wheat plant improves 4.1 times compared with control group.
Embodiment 4
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the microbial composite bacteria include following matter
The strain of amount part: 70 parts of azospirillum, 65 parts of pseudomonad, 30 parts of bacillus, 25 parts of actinomyces, 25 parts of yeast.
The azospirillum is made of Azospirillum brasilense and production rouge azospirillum with the ratio of mass parts 1:1, described
Bacillus bacillus licheniformis and Paenibacillus polymyxa formed with the ratio of mass parts 1:1, the streptomycete is by micro-
White yellow streptomycete composition, one of described yeast Kluyveromyces fragilis or more than one bacterial strain composition.
Compared with control group experimental result, after applying the complex micro organism fungicide in this example, after wheat cultivation 170d, often
The grain weight of strain wheat increases 4.3 times compared with control group, and the tiller number of every plant of wheat plant increases 2.2 times compared with control group,
The seed number of every plant of wheat plant improves 4.2 times compared with control group.
Embodiment 5
A kind of complex micro organism fungicide of enhancing salt tolerance of wheat of the invention, the microbial composite bacteria include following matter
The strain of amount part: 65 parts of azospirillum, 63 parts of pseudomonad, 33 parts of bacillus, 21 parts of actinomyces, 29 parts of yeast.
The azospirillum is made of Azospirillum brasilense, and the bacillus is made of bacillus subtilis, institute
By forming in grey streptomyces verticillus, the yeast is made of the streptomycete stated Candida.
Compared with control group experimental result, after applying the complex micro organism fungicide in this example, after wheat cultivation 170d, often
The grain weight of strain wheat increases 4.5 times compared with control group, and the tiller number of every plant of wheat plant increases 2.4 times compared with control group,
The seed number of every plant of wheat plant improves 4.6 times compared with control group.
In areal, 1200 wheat plants are randomly choosed, are randomly divided into 6 groups, every group 200, respectively with above-mentioned reality
Composite bacteria agent in example, control group do not apply, and it is 13dSm that selected wheat plant, which is planted in soil conductivity (EC),-1's
In soil, wheat planting experiment is carried out, dose is identical and other planting conditions are identical, collects sample after cultivating 170d, analyzes
The grain weight of wheat, tiller number and seed number.
Claims (8)
1. a kind of complex micro organism fungicide and preparation method thereof for enhancing salt tolerance of wheat, which is characterized in that the microorganism is multiple
Combined bacteria includes the strain of following mass parts: 50-80 parts of azospirillum, 45-70 parts of pseudomonad, and 20-40 parts of bacillus, strepto-
15-30 parts of bacterium, 20-35 parts of yeast.
2. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1, which is characterized in that described micro-
Biological compound fungi includes the strain of following mass parts: 60-70 parts of azospirillum, 50-65 parts of pseudomonad, and bacillus 30-38
Part, 18-25 parts of actinomyces, 25-33 parts of yeast.
3. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1, which is characterized in that described micro-
Biological compound fungi includes the strain of following mass parts: 65 parts of azospirillum, 63 parts of pseudomonad, and 33 parts of bacillus, actinomyces
21 parts, 29 parts of yeast.
4. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1-3, feature exist
In the azospirillum is made of one or more of Azospirillum brasilense, production rouge azospirillum bacterial strain.
5. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1-3, feature exist
In the bacillus is by bacillus thuringiensis, bacillus subtilis, bacillus licheniformis, Paenibacillus polymyxa
More than one bacterial strain composition.
6. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1-3, feature exist
In, the streptomycete by giving birth to melanosporin, avermectin streptomycete, Streptomycesalbidoflhaving, one in grey streptomyces verticillus
Kind or more bacterial strain composition.
7. a kind of complex micro organism fungicide for enhancing salt tolerance of wheat according to claim 1-3, feature exist
In the yeast is made of the bacterial strain of one or more of Candida, Kluyveromyces fragilis.
8. preparing a kind of preparation method for the complex micro organism fungicide for enhancing salt tolerance of wheat, feature described in claim 1 to exist
In, comprising the following steps:
(1) various purified strains are taken out from the inclined-plane of preservation, is inoculated into prepares and sterilized training respectively
It supports in base, the inoculum concentration of each strain is 3% by mass percentage, is placed under conditions of 30 DEG C, 180rpm/min oscillation training
Support 36-48h；
(2) cultured bacterial strain is diluted to 5 × 109A/ml, by 5 × 109Bacterium solution is adsorbed on carrier by the ratio of a/g, it
It is mixed afterwards in the strain ratio of a microbial inoculum, obtains complex micro organism fungicide；
(3) the mixed bacteria culture solution for completing preparation collects precipitating by centrifugation, then will precipitate drying, obtains Mixed Microbes powder.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111040967A (en) * | 2019-12-24 | 2020-04-21 | 鞍钢集团矿业有限公司 | Soda saline-alkali soil modifier containing combined microbial inoculum and application thereof |
| CN111057665A (en) * | 2019-12-11 | 2020-04-24 | 安徽农业大学 | Cellulose degrading bacterium n3 for producing IAA and application thereof |
| CN111073831A (en) * | 2019-12-24 | 2020-04-28 | 鞍钢集团矿业有限公司 | Compound microbial agent and application thereof |
| CN111088168A (en) * | 2019-12-24 | 2020-05-01 | 鞍钢集团矿业有限公司 | Saline-alkali soil modifier containing complex microbial inoculum and preparation method and application thereof |
| CN111117639A (en) * | 2019-12-24 | 2020-05-08 | 鞍钢集团矿业有限公司 | Soda saline-alkali soil modifier containing microorganisms and modifying method |
| CN112501061A (en) * | 2020-11-27 | 2021-03-16 | 广东丽豪生物农业有限公司 | Microbial agent for wheat planting |
| CN114717142A (en) * | 2022-03-09 | 2022-07-08 | 山东劲牛集团股份有限公司 | Preparation and application of streptomycete complex microbial inoculum |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111057665A (en) * | 2019-12-11 | 2020-04-24 | 安徽农业大学 | Cellulose degrading bacterium n3 for producing IAA and application thereof |
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| CN111040967A (en) * | 2019-12-24 | 2020-04-21 | 鞍钢集团矿业有限公司 | Soda saline-alkali soil modifier containing combined microbial inoculum and application thereof |
| CN111073831A (en) * | 2019-12-24 | 2020-04-28 | 鞍钢集团矿业有限公司 | Compound microbial agent and application thereof |
| CN111088168A (en) * | 2019-12-24 | 2020-05-01 | 鞍钢集团矿业有限公司 | Saline-alkali soil modifier containing complex microbial inoculum and preparation method and application thereof |
| CN111117639A (en) * | 2019-12-24 | 2020-05-08 | 鞍钢集团矿业有限公司 | Soda saline-alkali soil modifier containing microorganisms and modifying method |
| CN111073831B (en) * | 2019-12-24 | 2022-02-18 | 鞍钢集团矿业有限公司 | Compound microbial agent and application thereof |
| CN111040967B (en) * | 2019-12-24 | 2022-02-18 | 鞍钢集团矿业有限公司 | Soda saline-alkali soil modifier containing combined microbial inoculum and application thereof |
| CN111088168B (en) * | 2019-12-24 | 2023-04-07 | 鞍钢集团矿业有限公司 | Saline-alkali soil modifier containing complex microbial inoculum and preparation method and application thereof |
| CN112501061A (en) * | 2020-11-27 | 2021-03-16 | 广东丽豪生物农业有限公司 | Microbial agent for wheat planting |
| CN112501061B (en) * | 2020-11-27 | 2023-04-11 | 广东丽豪生物农业有限公司 | Microbial agent for wheat planting |
| CN114717142A (en) * | 2022-03-09 | 2022-07-08 | 山东劲牛集团股份有限公司 | Preparation and application of streptomycete complex microbial inoculum |
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