CN116114663B - Black soldier fly breeding method - Google Patents
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- CN116114663B CN116114663B CN202310149992.3A CN202310149992A CN116114663B CN 116114663 B CN116114663 B CN 116114663B CN 202310149992 A CN202310149992 A CN 202310149992A CN 116114663 B CN116114663 B CN 116114663B
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 67
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 241000238631 Hexapoda Species 0.000 claims abstract description 103
- 238000009423 ventilation Methods 0.000 claims abstract description 26
- 235000013601 eggs Nutrition 0.000 claims abstract description 18
- 230000012447 hatching Effects 0.000 claims abstract description 10
- 239000002344 surface layer Substances 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 53
- 238000005057 refrigeration Methods 0.000 claims description 27
- 238000003860 storage Methods 0.000 claims description 20
- SPFMQWBKVUQXJV-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;hydrate Chemical compound O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O SPFMQWBKVUQXJV-BTVCFUMJSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 18
- 230000007613 environmental effect Effects 0.000 claims description 13
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 241000037488 Coccoloba pubescens Species 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 238000007790 scraping Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 230000000052 comparative effect Effects 0.000 description 20
- 230000003213 activating effect Effects 0.000 description 17
- 238000004321 preservation Methods 0.000 description 6
- 241000382353 Pupa Species 0.000 description 4
- 239000010806 kitchen waste Substances 0.000 description 4
- 241001481656 Stratiomyidae Species 0.000 description 3
- 230000019617 pupation Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000709334 Hermetia Species 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000005059 dormancy Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002910 solid waste Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
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- Molecular Biology (AREA)
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- Catching Or Destruction (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a black soldier fly breeding method, which comprises the following steps: step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed; secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night; taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces; fourthly, refrigerating for 15-20 days, and feeding a small amount of feed in the first step after the seedlings are all active; and fifthly, after the fodder is taken by the young plants, refrigerating again, and repeating the fourth step.
Description
Technical Field
The invention relates to the technical field of hermetia illucens cultivation, in particular to a cultivation method of hermetia illucens.
Background
Hermetia illucens, genus hermetia. The hermetia fully metamorphosis insects are subjected to four insect states, namely eggs, larvae, pupae and adults, a pre-pupal period exists between the larvae and the pupae, and the pre-pupal has the habit of moving food to find a proper place for pupation. The life generation of the hermetia illucens is 28-40 days, the life cycle has high elasticity, the hermetia illucens is mainly in the pre-pupation stage, and the pre-pupation state of the hermetia illucens can be maintained for more than 6 months under a proper low-temperature state. Therefore, in the production process of the hermetia illucens, the production amount of the hermetia illucens is regulated by putting the pre-pupa into a refrigerator for refrigeration. The black soldier fly has the characteristics of wide feeding habit, high conversion rate, strong stress resistance, high biological safety and the like, and becomes one of the main selection insects for treating kitchen waste at present. The stability of the supply of the black soldier fly seedlings is the key for guaranteeing the stability of the kitchen waste treatment system. The adult black soldier flies have strict environmental requirements, and unsuitable humiture, adult density and pre-pupa quality can influence the fertility of the adult.
Such as the elevated temperatures nationwide in the summer of 2022, result in undersupply of eggs from various places. The patent ZL2021106759006 'a method for culturing hermetia illucens' reports a method for refrigerating hermetia illucens eggs and dormancy of pre-pupa so as to regulate the egg quantity of the hermetia illucens, but both methods have certain hysteresis, and the time from the development of the refrigerated eggs and the dormancy of the pre-pupa to the development of the hermetia illucens seedlings capable of treating kitchen waste is 10-30 days. Therefore, the preservation technology of the hermetia illucens seedlings is provided, and when the external climate environment is unfavorable for mating and spawning of adults, the quick-taking seedlings can better ensure the stability of kitchen waste disposal.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a black soldier fly breeding method for solving the problems in the background art.
The invention solves the technical problems by adopting the following technical scheme:
the invention provides a black soldier fly breeding method, which comprises the following steps:
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces;
fourthly, when the container is refrigerated for 15 to 20 days, taking out the container and placing the container in an environment of 25 to 30 ℃, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, and uniformly putting the fed feed, wherein the fed feed amount is 20 to 35 percent of that of the first step;
and fifthly, after the feed is completely taken by the young seedlings, refrigerating again when the moisture of the feed residues is 40% -50%, and repeating the fourth step.
Preferably, the feed comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large leaf bran.
Preferably, the concentration of the glucose water is 1 per mill, and the glucose water and the large She Fupi are uniformly stirred by a special mixing and stirring device when the feed is mixed.
Preferably, when the hermetia illucens is hatched in the first step, eggs are placed in a container, so that the temperature in the container is kept at 26-30 ℃ and hatching is performed for 3-5 days under the condition that the relative humidity is 75% -85%.
Preferably, the inoculation rate in the first step is that 200000-250000 hermetia illucens initially hatched larvae are inoculated on 1kg of feed, the environmental temperature is controlled at 28-30 ℃ in the cultivation process, the feed temperature is controlled at 28-35 ℃, and the environmental humidity is controlled at 50% -80%.
Preferably, the ventilation measure in the second step is to open an axial flow fan to circulate air, so that the water content of feed residues is reduced to 40% -50%, the surface of the insect body is dry, the weight of the insect seedlings is 1.5-3 mg/strip, and the water content of the insect seedlings is 70% -80%.
Preferably, the insect seedlings in the third step can hide under the feed residues due to light repellency, and the amount of the taken feed residues is 60% -80% of the total residue amount on the surfaces of the insect seedlings.
Preferably, the specific refrigeration treatment measures in the third step are as follows:
firstly, placing 4-6 cm thick seedlings and 1-1.5 cm thick feed residues into a container, and covering 240 mesh gauze on the upper part of the container, wherein the thickness of the seedlings is 40% -50% of the height of the container, and the aspect ratio of the container is (0.5-3): (0.5-3): 1;
and secondly, storing the container in a cold storage at 12-15 ℃, and taking care of avoiding a fan outlet of the cold storage and avoiding the container from being directly blown by a fan.
And thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
Preferably, in the fifth step, when the young seedling is to be used, the young seedling is taken out, the fourth step is repeated, and after the young seedling feeds the feed, the young seedling is put into production for use.
Compared with the prior art, the culturing method of the hermetia illucens has the following beneficial effects:
according to the invention, the hatched hermetia illucens seedlings and the feed residues are refrigerated together, so that the hermetia illucens seedlings enter a low-temperature low-oxygen dormant state and are in a relatively constant humidity environment, meanwhile, the seedlings are activated and fed at intervals, the hermetia illucens seedlings can be directly put into organic solid wastes when in use, and when not in use, some feed residues can be scraped off, and the hermetia illucens seedlings and the feed residues are refrigerated together again.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a black soldier fly breeding method, which comprises the following steps:
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces;
fourthly, when the container is refrigerated for 15 to 20 days, taking out the container and placing the container in an environment of 25 to 30 ℃, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, and uniformly putting the fed feed, wherein the fed feed amount is 20 to 35 percent of that of the first step;
and fifthly, after the feed is completely taken by the young seedlings, refrigerating again when the moisture of the feed residues is 40% -50%, and repeating the fourth step.
The feed of the embodiment comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large leaf bran.
The concentration of glucose water in this example was 1% and the glucose water and large She Fupi were stirred evenly by a proprietary mixing stirring device when the feed was mixed.
In the first step of the embodiment, the black soldier fly is hatched by placing the eggs in a container, and the temperature in the container is kept at 26-30 ℃ and the relative humidity is 75-85% for 3-5 days.
The inoculation rate in the first step of the embodiment is that 200000-250000 hermetia illucens initially hatched larvae are inoculated on 1kg of feed, the environmental temperature is controlled at 28-30 ℃ in the cultivation process, the feed temperature is controlled at 28-35 ℃, and the environmental humidity is controlled at 50% -80%.
The ventilation measure in the second step of the embodiment is to turn on an axial flow fan to circulate air, so that the water content of feed residues is reduced to 40% -50%, the surface of the insect body is dry, the weight of the insect seedlings is 1.5-3 mg/strip, and the water content of the insect seedlings is 70% -80%.
In the third step of the embodiment, the insect seedlings can hide under the feed residues due to light repellency, and the amount of the taken feed residues is 60% -80% of the total residue amount on the surfaces of the insect seedlings.
The specific measures for the refrigeration treatment in the step three of the present embodiment are as follows:
firstly, placing 4-6 cm thick seedlings and 1-1.5 cm thick feed residues into a container, and covering 240 mesh gauze on the upper part of the container, wherein the thickness of the seedlings is 40% -50% of the height of the container, and the aspect ratio of the container is (0.5-3): (0.5-3): 1;
and secondly, storing the container in a cold storage at 12-15 ℃, and taking care of avoiding a fan outlet of the cold storage and avoiding the container from being directly blown by a fan.
And thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
In the fifth step of the embodiment, when the seedlings are to be used, the seedlings are taken out, the fourth step is repeated, and after the feed is taken out by the seedlings, the seedlings are put into production for use.
Example 1.
The invention provides a black soldier fly breeding method, which comprises the following steps:
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces;
taking out the container and placing the container in an environment of 25 ℃ after refrigerating for 15 days, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, uniformly putting the fed feed, and feeding the feed with the feed amount of 20% of the feed amount in the first step;
and fifthly, after the feed is completely taken by the young seedlings, refrigerating again when the moisture of the feed residues is 40%, and repeating the step four.
The feed of the embodiment comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large leaf bran.
The concentration of glucose water in this example was 1% and the glucose water and large She Fupi were stirred evenly by a proprietary mixing stirring device when the feed was mixed.
In the first step of the embodiment, the black soldier fly is hatched by placing the eggs in a container, and the temperature in the container is kept at 28 ℃ and the relative humidity is 75% for 4 days.
The inoculation rate in the first step of the embodiment is that about 220000 black soldier fly initially hatched larvae are inoculated on 1kg of feed, the environmental temperature is controlled at 28 ℃ in the cultivation process, the feed temperature is controlled at 28 ℃, and the environmental humidity is controlled at 50%.
The ventilation measure in the second step of the embodiment is to turn on an axial flow fan to circulate air, so that the water content of feed residues is reduced to 45.2%, the surface of the insect body is dry, the weight of the insect seedlings is 2 mg/strip, and the water content of the insect seedlings is 76.8%.
In the third step of the embodiment, the insect seedlings can avoid the lower part of the feed residues due to light repellency, and the amount of the taken feed residues is 60% of the total residue amount on the surfaces of the insect seedlings.
The specific measures for the refrigeration treatment in the step three of the present embodiment are as follows:
firstly, placing 5 cm thick seedlings and 1 cm thick feed residues in a container, and covering 240 mesh gauze above the container, wherein the thickness of the seedlings is 40% of the height of the container, and the aspect ratio of the container is 0.5:0.5:1;
and secondly, storing the container in a refrigeration house at 13 ℃, and taking care of avoiding a fan outlet of the refrigeration house to avoid the container from being directly blown by a fan.
And thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
In the fifth step of the embodiment, when the seedlings are to be used, the seedlings are taken out, the fourth step is repeated, and after the feed is taken out by the seedlings, the seedlings are put into production for use.
Example 2.
The invention provides a black soldier fly breeding method, which comprises the following steps:
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces;
taking out the container and placing the container in an environment of 28 ℃ after cold storage for 18 days, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, uniformly putting the fed feed, and feeding the feed with the feed amount of 30% of the feed amount in the first step;
and fifthly, after the feed is completely taken by the young seedlings, refrigerating again when the moisture of feed residues is 45%, and repeating the step four.
The feed of the embodiment comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large leaf bran.
The concentration of glucose water in this example was 1% and the glucose water and large She Fupi were stirred evenly by a proprietary mixing stirring device when the feed was mixed.
In the first step of the embodiment, the black soldier fly is hatched by placing the eggs in a container, and the temperature in the container is kept at 30 ℃ and the relative humidity is 80% for 3 days.
The inoculation rate in the first step of the embodiment is that about 250000 black soldier flies are inoculated on 1kg of feed, the environmental temperature is controlled at 29 ℃ in the cultivation process, the feed temperature is controlled at 30 ℃, and the environmental humidity is controlled at 66%.
The ventilation measure in the second step of the embodiment is to turn on an axial flow fan to circulate air, so that the water content of feed residues is reduced to 47.1%, the surface of the insect body is dry, the weight of the insect seedlings is 1.5 mg/strip, and the water content of the insect seedlings is 77.4%.
In the third step of the embodiment, the insect seedlings can avoid the lower part of the feed residues due to light repellency, and the amount of the taken feed residues is 70% of the total residue amount on the surfaces of the insect seedlings.
The specific measures for the refrigeration treatment in the step three of the present embodiment are as follows:
firstly, placing 4 cm thick seedlings and 1 cm thick feed residues in a container, and covering 240 mesh gauze above the container, wherein the thickness of the seedlings is 45% of the height of the container, and the aspect ratio of the container is 2:2:1;
step two, storing the container in a refrigeration house at 14 ℃, and taking care of avoiding the outlet of a fan of the refrigeration house so as to avoid the container from being directly blown by the fan;
and thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
In the fifth step of the embodiment, when the seedlings are to be used, the seedlings are taken out, the fourth step is repeated, and after the feed is taken out by the seedlings, the seedlings are put into production for use.
Example 3.
The invention provides a black soldier fly breeding method, which comprises the following steps:
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of about 1 cm on the surface layer, and refrigerating the insect seedlings with the small amount of feed residues coated on the surfaces;
taking out the container and placing the container in an environment of 30 ℃ after cold storage for 20 days, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, uniformly putting the fed feed, and enabling the fed feed amount to be 35% of the feed amount in the first step;
and fifthly, after the feed is completely taken by the young seedlings, refrigerating again when the moisture of the feed residues is 50%, and repeating the step four.
The feed of the embodiment comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large leaf bran.
The concentration of glucose water in this example was 1% and the glucose water and large She Fupi were stirred evenly by a proprietary mixing stirring device when the feed was mixed.
In the first step of the embodiment, the black soldier fly is hatched by placing the eggs in a container, and the temperature in the container is kept at 26 ℃ and the relative humidity is 85% for 5 days.
The inoculation rate in the first step of the embodiment is that about 200000 hermetia illucens initially hatched larvae are inoculated on 1kg of feed, the environmental temperature is controlled at 30 ℃ in the cultivation process, the feed temperature is controlled at 35 ℃, and the environmental humidity is controlled at 80%.
The ventilation measure in the second step of the embodiment is to turn on an axial flow fan to circulate air, so that the water content of feed residues is reduced to 50%, the surface of the insect body is dry, the weight of the insect seedlings is 2.5 mg/strip, and the water content of the insect seedlings is 74.3%.
In the third step of the embodiment, the insect seedlings can avoid the lower part of the feed residues due to light repellency, and the amount of the taken feed residues is 80% of the total residue amount on the surfaces of the insect seedlings.
The specific measures for the refrigeration treatment in the step three of the present embodiment are as follows:
firstly, placing 6 cm thick seedlings in a container, 1.5 cm thick feed residues, and covering 240 mesh gauze on the upper part of the container, wherein the thickness of the seedlings is 50% of the height of the container, and the aspect ratio of the container is 3:3:1;
step two, storing the container in a cold storage at 12 ℃, and taking care of avoiding a fan outlet of the cold storage so as to avoid the container from being directly blown by a fan;
and thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
In the fifth step of the embodiment, when the seedlings are to be used, the seedlings are taken out, the fourth step is repeated, and after the feed is taken out by the seedlings, the seedlings are put into production for use.
Comparative example 1.
The difference from example 1 is the weight of the larvae.
Comparative example 2.
Unlike example 1, the water content of the feed residue was increased.
Comparative example 3 and comparative example 4.
The difference from example 1 is the thickness of the refrigerated seedlings.
Comparative example 5 and comparative example 6.
The difference from example 1 is the thickness of the feed residue.
Comparative example 7 and comparative example 8.
The difference from example 1 is the water content of the feed residues.
Comparative example 9 and comparative example 10.
The number of the cover gauze was different from that of example 1.
Comparative example 11.
Unlike example 1, the refrigerator was used.
Comparative example 12 and comparative example 13.
The difference from example 1 is the refrigeration temperature.
Comparative example 14.
Unlike example 1, the activation cycle was varied.
Comparative example 15.
Unlike example 1, which is a container aspect ratio example, comparative example 15 has an aspect ratio of 4:4:1.
The products of examples 1-3 and comparative examples 1-15 were subjected to performance testing;
the insect seedlings hatched in the same period are selected to be divided into 18 groups, and the inoculation rate of 1kg of feed is as follows: example 1 vaccinated about 220000, example 2 vaccinated about 250000, example 3 vaccinated about 200000, comparative example 1 vaccinated about 300000, and the remaining comparative examples 2-14 vaccinated about 220000. The 1-18 groups correspond to examples 1-3 and comparative examples 1-14 in order, and the obtained seedlings have the results shown in the following table.
Sequence number | Preservation method | Survival rate > 80% of preservation time |
1 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold Tibet worm seedling thickness 5 cm, feedingThickness of residue 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 15 days Activating once. | Day > 90 |
2 | 1.5 mg/strip of insect seedlings, 77.4 percent of insect water content, the thickness of the refrigerated insect seedlings is 4 cm, and the thickness of the feed residues is 4 cm 1 cm, feed residue water content 47.1%, container The aspect ratio of (2) to (2:2:1) ratio, the cover gauze 240 meshes, refrigerating at 14deg.C, taking out every 15 days Activating once. | Day > 90 |
3 | 2.5 mg/strip of insect seedlings, 74.3 percent of insect water content, 6 cm of refrigerated insect seedlings and 6 cm of feed residues 1.5 cm, the feed residue water content is 44.5%, and the capacity is The length to width ratio of the device is 3:3:1, and the device is covered with a gauze 240 meshes, refrigerating at 12 deg.C, taking out every 20 days Activating once. | Day > 90 |
4 | 0.8 mg/strip of insect seedlings, 76.4 percent of insect water content, the thickness of the refrigerated insect seedlings is 5 cm, and the thickness of the feed residues is 5 cm 1 cm, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 15 days Activating once. | Less than 30 days |
5 | 2 mg/strip of insect seedlings, water content of insect is 84.6%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn The mesh is 240 meshes, the refrigeration temperature is 13 ℃,take every 15 days Activating once. | Less than 60 days |
6 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 2 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 15 days Activating once. | Less than 30 days |
7 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 8 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 15 days Activating once. | Less than 60 days |
8 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 5 cm 0.5 cm, the feed residue water content is 45.2%, and the capacity is The aspect ratio of the device is 0.5:0.5:1, covering 240 mesh gauze, 13 ℃ cold storage temperature, 15 days each time Taking out and activating once. | Less than 15 days |
9 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm and the thickness of the feed residue is 6 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 15 days Activating once. | Less than 45 days |
10 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Cm, feed residue water content 70%, container Aspect ratio of 0.5:0.5:1, cover gauze 240 meshes, refrigerating at 13 deg.C, taking out every 15 days Activating once. | Less than 15 days |
11 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Cm, feed residue water content 30%, container Aspect ratio of 0.5:0.5:1, cover gauze 240 meshes, refrigerating at 13 deg.C, taking out every 15 days Activating once. | Less than 45 days |
12 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 100 mesh net, 13 deg.C cold storage temperature, every 15 days Activating once. | Less than 45 days |
13 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 400 mesh net, 13 deg.C cold storage temperature, every 15 days Activating once. | Less than 30 days |
14 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold Tibet wormSeedling thickness 5 cm, feed residue thickness 1 Centimeter, feed residue water content 45.2%, container The length-width ratio of the sealing bag is 0.5:0.5:1 Preserving at 13 deg.C, taking out living every 15 days And (5) performing once treatment. | Less than 15 days |
15 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn The net is 240 meshes, the refrigerating temperature is 8 ℃, and the net is taken out every 15 days Activating once. | Less than 30 days |
16 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, refrigerating temperature 20 deg.C, taking every 15 days Activating once. | Less than 30 days |
17 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container Aspect ratio of 0.5:0.5:1, cover yarn 240 mesh net, 13 ℃ cold storage temperature, every 30 days Activating once. | Less than 60 days |
18 | 2 mg/strip of insect seedling, water content of insect is 76.8%, cold The thickness of the Tibetan insect seedling is 5 cm, and the thickness of the feed residue is 1 Centimeter, feed residue water content 45.2%, container The aspect ratio of (2) is 4:4:1, the cover gauze 240 mesh, coldThe storage temperature is 13 ℃, and the product is taken out every 15 days Activating once. | Less than 45 days |
As can be seen from groups 1-3; the black soldier fly breeding method prepared by the method has remarkable effect, wherein the maximum preservation period of the black soldier fly seedlings in refrigeration is more than 3 months, and the preservation survival rate is more than 80%.
From group 4, the weight of the young insects is too small, the young insects are more easy to die, and the refrigerating and preserving period of the young hermetia illucens is reduced;
as can be seen from the group 5, the water content of the young insects is too high, and the young black soldier flies can be reduced due to the excessive wetting of the insects;
from groups 6-7, the refrigerating shelf life of the hermetia illucens seedlings can be reduced when the thickness of the refrigerated insect seedlings is too high or too low;
as can be seen from groups 8-9, the thickness of the refrigerated feed residues is too high or too low, so that the refrigerated shelf life of the hermetia illucens seedlings is reduced;
from groups 10-11, the water content of the refrigerated feed residues is too high or too low, so that the refrigerated shelf life of the hermetia illucens seedlings is reduced;
from groups 12-13, the number of meshes of the gauze covered on the container is too high or too low, so that the refrigerating shelf life of the hermetia illucens seedlings is reduced;
as can be seen from the 14 th group, when the container for refrigerating the young insects is a sealed bag, the refrigerating shelf life of the young hermetia illucens is reduced;
from groups 15-16, the refrigerating temperature of the young soldiers is too high or too low, so that the refrigerating shelf life of the young soldiers is reduced;
as can be seen from group 17, the longer activation period of the refrigerated insect seedlings can reduce the refrigerated shelf life of the hermetia illucens seedlings.
As can be seen from group 18, when the aspect ratio of the container length is not suitable, the refrigerated shelf life of the hermetia illucens seedlings is reduced.
The table shows that the insect seedling has 1.5-2.5 mg/strip, the insect moisture content is 70-80%, the thickness of the refrigerated insect seedling is 4-6 cm, the thickness of the feed residue is 1-1.5 cm, the moisture content of the feed residue is 40-50%, the screen mesh is covered with 240 meshes, the refrigerating temperature is 12-15 ℃, and the best preservation method for the black soldier fly seedling is obtained once every 15-20 days.
The innovation point of the invention is that:
the method comprises the steps of refrigerating the hatched hermetia illucens seedlings together with feed residues, enabling the hermetia illucens seedlings to enter a low-temperature low-oxygen dormant state, enabling the hermetia illucens seedlings to be in a relatively constant humidity environment, providing nutrition and moisture for the seedlings at intervals, enabling the seedlings to be activated and fed, directly placing the seedlings into organic solid wastes when the method is used, refrigerating the hermetia illucens seedlings together with the feed residues again when the method is not used, not only reducing the raising cost, but also keeping the longest keeping period of the refrigeration of the hermetia illucens seedlings for more than 3 months, keeping the survival rate for more than 80%, taking along with use and keeping the young hermetia illucens seedlings at low cost.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (3)
1. The black soldier fly breeding method is characterized by comprising the following steps of;
step one, preparing feed, hatching hermetia illucens eggs, and inoculating the newly hatched larvae to the feed;
secondly, after the feed is completely ingested by the larvae on the 5 th day, adopting ventilation measures to remove some moisture in feed residues, and then closing ventilation and standing for one night;
taking out most of feed residues, discarding the feed residues, leaving the feed residues with the thickness of 1 cm on the surface layer, and refrigerating the insect seedlings with the surfaces coated with a small amount of feed residues;
fourthly, when the container is refrigerated for 15-20 days, taking out the container and placing the container in an environment of 25-30 ℃, scraping surface residues, lightly turning over seedlings, feeding a small amount of feed in the first step after the seedlings are all active, and uniformly putting the fed feed, wherein the fed feed amount is 20% -35% of that of the feed in the first step;
fifthly, after the fodder is completely eaten by the young seedlings, refrigerating again when the water content of fodder residues is 40% -50%, and repeating the fourth step;
the feed comprises the following raw materials in parts by mass: 2.5 parts of glucose water and 1 part of large-leaf bran;
the concentration of glucose water is 1 per mill, and the glucose water and the large She Fupi are uniformly stirred by special mixing stirring equipment when the feed is mixed;
placing eggs in a container when hatching hermetia illucens in the first step, and keeping the temperature in the container at 26-30 ℃ and hatching for 3-5 days under the condition that the relative humidity is 75% -85%;
the inoculation rate in the first step is that 200000-250000 black soldier fly initially hatched larvae are inoculated on 1kg of feed, the environmental temperature is controlled at 28-30 ℃ in the cultivation process, the feed temperature is controlled at 28-35 ℃, and the environmental humidity is controlled at 50-80%;
the ventilation measure in the second step is to open an axial flow fan to circulate air, ensure that the water content of feed residues is reduced to 40% -50%, the surface of the insect body is dry, the weight of the insect seedlings is 1.5-3 mg/strip, and the water content of the insect seedlings is 70% -80%; the specific refrigeration treatment measures in the third step are as follows:
firstly, placing 4-6 cm thick seedlings and 1-1.5 cm thick feed residues into a container, and covering 240 mesh gauze on the upper part of the container, wherein the thickness of the seedlings is 40% -50% of the height of the container, and the aspect ratio of the container is (0.5-3): (0.5-3): 1;
step two, storing the container in a cold storage at the temperature of 12-15 ℃ and taking care of avoiding the fan outlet of the cold storage so as to avoid the container from being directly blown by a fan;
and thirdly, the sealing performance of the refrigeration house is strong, the ventilation of the fan is required to be carried out for 15 minutes every day, and the air in the refrigeration house is kept fresh.
2. The method for culturing hermetia illucens according to claim 1, wherein the young insects in the third step can hide under the feed residues due to light repellency, and the amount of the feed residues taken out is 60% -80% of the total residue amount on the surfaces of the young insects.
3. The method for raising hermetia illucens according to claim 1, wherein in the fifth step, when the young seedling is to be used, the young seedling is taken out, the fourth step is repeated, and after the young seedling is fed with the feed, the young seedling is put into production for use.
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