CN116098150A - Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof - Google Patents
Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN116098150A CN116098150A CN202211580921.0A CN202211580921A CN116098150A CN 116098150 A CN116098150 A CN 116098150A CN 202211580921 A CN202211580921 A CN 202211580921A CN 116098150 A CN116098150 A CN 116098150A
- Authority
- CN
- China
- Prior art keywords
- powder
- grubs
- microbial preparation
- leaf powder
- core layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 83
- 238000002360 preparation method Methods 0.000 title claims abstract description 77
- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 33
- 239000000843 powder Substances 0.000 claims abstract description 143
- 239000012792 core layer Substances 0.000 claims abstract description 60
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000000661 sodium alginate Substances 0.000 claims abstract description 37
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 37
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 37
- 108010068370 Glutens Proteins 0.000 claims abstract description 31
- 239000010410 layer Substances 0.000 claims abstract description 28
- 244000144730 Amygdalus persica Species 0.000 claims abstract description 25
- 241000207783 Ipomoea Species 0.000 claims abstract description 25
- 240000000249 Morus alba Species 0.000 claims abstract description 25
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 25
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 25
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 25
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 25
- 235000006040 Prunus persica var persica Nutrition 0.000 claims abstract description 25
- 241000751139 Beauveria bassiana Species 0.000 claims abstract description 24
- 241001465752 Purpureocillium lilacinum Species 0.000 claims abstract description 24
- 239000011247 coating layer Substances 0.000 claims abstract description 23
- 239000000230 xanthan gum Substances 0.000 claims abstract description 23
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 23
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 23
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 23
- 229920002749 Bacterial cellulose Polymers 0.000 claims abstract description 21
- 108010022355 Fibroins Proteins 0.000 claims abstract description 21
- 239000005016 bacterial cellulose Substances 0.000 claims abstract description 21
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 8
- 239000008158 vegetable oil Substances 0.000 claims abstract description 8
- 239000001959 sucrose esters of fatty acids Substances 0.000 claims abstract description 3
- 235000010965 sucrose esters of fatty acids Nutrition 0.000 claims abstract description 3
- 239000000839 emulsion Substances 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 21
- 229930006000 Sucrose Natural products 0.000 claims description 18
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 18
- 239000000194 fatty acid Substances 0.000 claims description 18
- 229930195729 fatty acid Natural products 0.000 claims description 18
- 239000005720 sucrose Substances 0.000 claims description 18
- -1 sucrose fatty acid ester Chemical class 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 16
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 12
- 238000004132 cross linking Methods 0.000 claims description 10
- 239000011812 mixed powder Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 8
- 239000006187 pill Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 7
- 238000005096 rolling process Methods 0.000 claims description 7
- 239000003431 cross linking reagent Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 18
- 238000003860 storage Methods 0.000 abstract description 10
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000000575 pesticide Substances 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 27
- 239000002689 soil Substances 0.000 description 17
- 244000105624 Arachis hypogaea Species 0.000 description 16
- 235000020232 peanut Nutrition 0.000 description 16
- 239000003795 chemical substances by application Substances 0.000 description 15
- 239000003921 oil Substances 0.000 description 13
- 235000019198 oils Nutrition 0.000 description 13
- 235000020238 sunflower seed Nutrition 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 241000607479 Yersinia pestis Species 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 9
- 241000244206 Nematoda Species 0.000 description 9
- 230000000749 insecticidal effect Effects 0.000 description 8
- 235000017060 Arachis glabrata Nutrition 0.000 description 7
- 235000010777 Arachis hypogaea Nutrition 0.000 description 7
- 235000018262 Arachis monticola Nutrition 0.000 description 7
- 238000001723 curing Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000035784 germination Effects 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 241000243784 Meloidogyne arenaria Species 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000009331 sowing Methods 0.000 description 3
- 230000004763 spore germination Effects 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000005253 cladding Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000270708 Testudinidae Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a microbial preparation for preventing and controlling root-knot nematodes and grubs, which comprises a core layer, an intermediate layer and a coating layer; the core layer comprises paecilomyces lilacinus spore powder and beauveria bassiana spore powder; vegetable oil; bacterial cellulose; silk fibroin; xanthan gum; sucrose esters of fatty acids; the middle layer consists of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder; the coating layer consists of sodium alginate and gluten protein; the microbial preparation has good storage stability and long storage life; the compound pesticide has excellent control effect on root-knot nematodes and grubs, and can promote the growth of crops and improve the yield and quality of the crops.
Description
Technical Field
The invention belongs to the field of microbial preparations, and particularly relates to a microbial preparation for preventing and treating root knot nematodes and grubs, and a preparation method and application thereof.
Background
The root-knot nematode is taken as a plant parasitic nematode, and is mainly harmful to the root of plants, and is mainly characterized in that root nodules are formed on the root of the plants, so that the growth of the root system of the plants is poor, and after the root system of the plants is attacked by the nematode, the absorption of nutrients and moisture in soil is affected, so that the overground part of the plants is wilted, the growth is slow and short, the yield is low, and even the plants die; the crop is reduced in yield, and serious economic loss is brought to farmers;
grubs are larvae of scarves or tortoises, which can harm various crops, economic crops and flowers and seedlings, especially seeds, roots, tubers and seedlings which are loved to eat just sown are world underground pests, and have great harm to crops.
At present, most of the commonly used methods for preventing and treating root-knot nematodes and grubs adopt chemical agents; and different chemical agents are adopted for controlling different diseases respectively, one agent can only achieve the effect of controlling one disease and insect pest, the control effect of the agent is single, the chemical agent is easy to cause the drug resistance of the insect pest after long-term use, the drug effect is weakened, and meanwhile, the chemical agent inevitably pollutes soil and crops; causing pesticide residues in crops; in the prior art, microbial agents are adopted to prevent and treat insect pests, and the microbial agents are substances capable of generating insect pest resistance in the metabolic process of the microbial agents. However, the existing microbial agents are not ideal in storage stability, and the microbial agents are inactivated or lost along with the extension of storage time; the onset time is short, and the control effect of the microbial preparation on crop diseases and insect pests is reduced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a microbial preparation for preventing and treating root-knot nematodes and grubs, and the microbial preparation has good storage stability and long storage period; the compound pesticide has excellent control effect on root-knot nematodes and grubs, and can promote the growth of crops and improve the yield and quality of the crops.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer comprises paecilomyces lilacinus spore powder and beauveria bassiana spore powder; vegetable oil; bacterial cellulose; silk fibroin; xanthan gum; sucrose esters of fatty acids; the middle layer consists of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder; the coating layer consists of sodium alginate and gluten protein;
preferably, the weight ratio of each raw material in the core layer is as follows: 10-14 parts of paecilomyces lilacinus spore powder; 10-14 parts of beauveria bassiana spore powder; 20-28 parts of vegetable oil; 1-5 parts of bacterial cellulose; 0.5-1 part of silk fibroin; 1-1.4 parts of xanthan gum; 1-1.5 parts of sucrose fatty acid ester;
preferably, the weight ratio of the peach leaf powder to the mulberry leaf powder to the pharbitis seed powder to the lotus leaf powder is 1:1:1:1, a step of;
preferably, the particle size of the peach leaf powder, the mulberry leaf powder, the pharbitis seed powder and the lotus leaf powder is 100-300 meshes;
preferably, the weight ratio of the sodium alginate to the gluten protein in the coating layer is 1:0.01-0.05;
further, the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in vegetable oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, stirring uniformly, and obtaining emulsion; injecting the emulsion into a pill dropping machine, and dripping into condensed liquid to obtain a core layer;
(2) Firstly removing condensed liquid on the surface of a core layer, placing the core layer in mixed powder of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, placing in a cross-linking agent solution, and cross-linking, solidifying and drying to obtain a microbial preparation;
preferably, the condensed liquid is methyl silicone oil;
preferably, the cross-linking agent solution is sodium chloride solution;
preferably, the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%.
The microbial preparation for preventing and controlling the root-knot nematodes and the grubs is applied to preventing and controlling the root-knot nematodes and/or the grubs.
Advantageous effects
The microbial agent disclosed by the invention is reasonable in structural design, and not only ensures that the microbial agent has good stability and prolonged shelf life through the synergistic effect among the components, but also has long-acting control effect on root-knot nematodes and grubs; and simultaneously, the growth of crops can be promoted, and the yield is improved.
The invention discloses a microbial preparation, which comprises a three-layer structure, wherein a core layer consists of paecilomyces lilacinus spore powder, beauveria bassiana spore powder, vegetable oil, bacterial cellulose, silk fibroin, xanthan gum and sucrose fatty acid ester in a certain proportion; the paecilomyces lilacinus and beauveria bassiana spore powder which are effective active ingredients in the core layer are dispersed in the oil phase, and bacterial cellulose, silk fibroin, xanthan gum and sucrose fatty acid ester are contained in the paecilomyces lilacinus and beauveria bassiana spore powder, so that on one hand, the mechanical strength of the core layer material is ensured, and on the other hand, nutrients are provided for germination of later-stage spores; the xanthan gum enables the core layer to have certain viscosity, and is beneficial for the mixed powder of the middle layer peach leaf powder, mulberry leaf powder, pharbitis seed powder, lotus leaf powder and the like to adhere to the surface of the core layer; the middle layer is coated with a coating layer formed by sodium alginate/gluten protein under the crosslinking action of a crosslinking agent, so that the microbial preparation capable of preventing root-knot nematodes and grubs is prepared, the three-layer structure of the microbial preparation reduces the probability of contacting with the environment during the storage process of the strain, and the activity of the strain can be effectively maintained; the preservation period of the strain is remarkably improved;
the middle layer of the microbial preparation contains mixed powder of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, and has a certain insecticidal effect on one hand, and is synergistic with a core layer microbial agent to resist insects; on the other hand, after the microbial preparation is applied to the soil, nutrient substances can be provided for paecilomyces lilacinus and beauveria bassiana spore powder, so that the propagation of beneficial strains is promoted, and the pest control effect of the beneficial strains is improved.
The coating layer is obtained by crosslinking sodium alginate and gluten protein under a crosslinking agent; the elasticity and compactness of the coating layer are improved by adding the gluten protein; the coating layer is not easy to break, which is beneficial to keeping the activity of the effective components in the core layer; on the other hand, after the microbial preparation is applied into the soil, the microbial preparation can absorb water rapidly and expand without cracking, so that the microbial preparation is beneficial to keeping the long-acting effect of preventing and controlling plant diseases and insect pests.
Drawings
FIG. 1 is a graph of peanut growth in different treatment groups in a field trial of the present invention;
FIG. 2 is a graph of peanut results from different treatment groups in a field trial of the present invention;
Detailed Description
Example 1
A microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer consists of the following raw materials in parts by weight: 10 parts of paecilomyces lilacinus spore powder; 10 parts of beauveria bassiana spore powder; 20 parts of sunflower seed oil; 1 part of bacterial cellulose; 0.5 parts of silk fibroin; 1 part of xanthan gum; 1 part of fatty acid sucrose ester; the middle layer is prepared from peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in a weight ratio of 1:1:1:1, the composition is as follows; the coating layer consists of sodium alginate and gluten protein in a weight ratio of 1:0.01;
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, and mixing uniformly to obtain emulsion; injecting the emulsion into a pill dropping machine, and dripping the emulsion into condensed liquid methyl silicone oil to obtain a core layer;
(2) Firstly, removing methyl silicone oil on the surface of a core layer, placing the core layer in mixed powder (with the particle size of 100-300 meshes) of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, and placing in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%.
Example 2
A microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer consists of the following raw materials in parts by weight: 11 parts of paecilomyces lilacinus spore powder; 11 parts of beauveria bassiana spore powder; 22 parts of sunflower seed oil; 2 parts of bacterial cellulose; 0.6 parts of silk fibroin; 1.1 parts of xanthan gum; 2 parts of fatty acid sucrose ester; the middle layer is prepared from peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in a weight ratio of 1:1:1:1, the composition is as follows; the coating layer consists of sodium alginate and gluten protein in a weight ratio of 1:0.02;
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, and mixing uniformly to obtain emulsion; injecting the emulsion into a pill dropping machine, and dripping the emulsion into condensed liquid methyl silicone oil to obtain a core layer;
(2) Firstly, removing methyl silicone oil on the surface of a core layer, placing the core layer in mixed powder (with the particle size of 100-300 meshes) of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, and placing in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%.
Example 3
A microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer is prepared from the following raw materials in parts by weight: 12 parts of paecilomyces lilacinus spore powder; 12 parts of beauveria bassiana spore powder; 24 parts of sunflower seed oil; 3 parts of bacterial cellulose; 0.75 parts of silk fibroin; 1.2 parts of xanthan gum and 1.25 parts of sucrose fatty acid ester; the middle layer is prepared from peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in a weight ratio of 1:1:1:1, the composition is as follows; the coating layer consists of sodium alginate and gluten protein in a weight ratio of 1:0.03;
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, and mixing uniformly to obtain emulsion; injecting the emulsion into a pill dropping machine, and dripping the emulsion into condensed liquid methyl silicone oil to obtain a core layer;
(2) Firstly, removing methyl silicone oil on the surface of a core layer, placing the core layer in mixed powder (with the particle size of 100-300 meshes) of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, and placing in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%;
example 4
A microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer is prepared from the following raw materials in parts by weight: 13 parts of paecilomyces lilacinus spore powder; 13 parts of beauveria bassiana spore powder; 26 parts of sunflower seed oil; 4 parts of bacterial cellulose; 0.8 parts of silk fibroin; 1.4 parts of xanthan gum and 1.4 parts of sucrose fatty acid ester; the middle layer is prepared from peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in a weight ratio of 1:1:1:1, the composition is as follows; the coating layer consists of sodium alginate and gluten protein in a weight ratio of 1:0.04;
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, and mixing uniformly to obtain emulsion; injecting the emulsion into a pill dropping machine, and dripping the emulsion into condensed liquid methyl silicone oil to obtain a core layer;
(2) Firstly, removing methyl silicone oil on the surface of a core layer, placing the core layer in mixed powder (with the particle size of 100-300 meshes) of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, and placing in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%;
example 5
A microbial preparation for preventing and controlling root-knot nematodes and grubs comprises a core layer, an intermediate layer and a coating layer; the core layer consists of the following raw materials in parts by weight: 14 parts of paecilomyces lilacinus spore powder; 14 parts of beauveria bassiana spore powder; 28 parts of sunflower seed oil; 5 parts of bacterial cellulose; 1 part of silk fibroin; 1.4 parts of xanthan gum; 1.5 parts of sucrose fatty acid ester; the middle layer consists of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder (the weight ratio is 1:1:1:1); the coating layer consists of sodium alginate and gluten protein (weight ratio is 1:0.05);
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, and mixing uniformly to obtain emulsion; injecting the emulsion into a pill dropping machine, and dripping the emulsion into condensed liquid methyl silicone oil to obtain a core layer;
(2) Firstly, removing methyl silicone oil on the surface of a core layer, placing the core layer in mixed powder (with the particle size of 100-300 meshes) of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, and placing in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%;
comparative example 1
A microbial preparation for preventing and controlling root-knot nematodes and grubs, which comprises a core layer and a coating layer; the core layer is prepared from the following raw materials in parts by weight: 12 parts of paecilomyces lilacinus spore powder and 12 parts of beauveria bassiana spore powder; 24 parts of sunflower seed oil; 3 parts of bacterial cellulose; 0.75 parts of silk fibroin; 1.2 parts of xanthan gum and 1.25 parts of sucrose fatty acid ester; the coating layer consists of sodium alginate and gluten protein in a weight ratio of 1:0.03;
the preparation method of the microbial preparation specifically comprises the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in sunflower seed oil; sequentially adding bacterial cellulose, silk fibroin, xanthan gum and sucrose fatty acid ester into the mixture, and stirring and mixing the mixture uniformly to obtain emulsion; injecting the emulsion into dripping pill, and dripping into condensed liquid methyl silicone oil to obtain core layer;
(2) Removing methyl silicone oil on the surface of the core layer, spraying sodium alginate/gluten protein mixed solution on the surface, and then placing the mixture in 5wt% sodium chloride solution for crosslinking, curing and drying to obtain a microbial preparation;
wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%;
comparative example 2
Comparative example 2 was substantially the same as the microbial preparation obtained in example 3, except that gluten was not contained in the outermost layer of the microbial preparation in comparative example 2;
comparative example 3
Comparative example 3 was substantially the same as the microbial preparation obtained in example 3, except that the core layer of the microbial preparation in comparative example 3 did not contain xanthan gum; the adhesion of the core layer prepared in the comparative example 3 to the mixed powder of the middle peach leaf powder, the mulberry leaf powder, the pharbitis seed powder and the lotus leaf powder is poor, the middle layer is easy to fall off and is not favorable for cladding by the cladding layer, and the microbial preparation with complete surface is not easy to prepare and obtain;
performance detection
Storage stability of microbial formulations
The microbial preparation prepared in the embodiment 3 and the microbial preparation prepared in the comparative examples 1-2 are stored for 12 months at 25 ℃, and the germination rate of spores of the microorganisms is detected by periodically taking the microbial preparation, wherein the germination rate of spores refers to the sum of the germination rates of spores of paecilomyces lilacinus and beauveria bassiana; the test results are shown in Table 1.
TABLE 1 spore germination rate in microbial formulations over extended storage time
| 2 | 4 | 6 | 8 | 10 | 12 | |
| Example 3 | 97.5％ | 95.8％ | 94.3％ | 92.6％ | 90.8％ | 90.0％ |
| Comparative example 1 | 91.6％ | 83.1％ | 75.7％ | 72.6％ | 69.8％ | 57.2％ |
| Comparative example 2 | 89.1％ | 76.3％ | 71.3％ | 66.8％ | 51.4％ | 48.3％ |
As can be seen from the data in Table 1, the germination rate of spores can still reach more than 90% after the microbial preparation prepared in the embodiment 3 of the invention is stored for 12 months; exhibit good storage stability; in contrast, the spore germination rate was reduced in both comparative example 1 and comparative example 2, as compared to example 3.
Detection of insecticidal performance of microbial preparation
100 nematodes (second-instar larvae) are uniformly dispersed into 10kg of soil (the soil is subjected to high-temperature insecticidal treatment and does not contain root-knot nematodes), the microbial preparation prepared in the embodiment 3 and the comparative examples 1-2 is applied to the soil, water is applied to the soil, and the water content of the soil is kept at 18-25%; after 30 days of application of the microbial formulation; mortality of nematodes was recorded and the results are reported in table 2.
Uniformly dispersing 100 grubs into 10kg of soil (the soil is subjected to high-temperature insecticidal treatment and does not contain grubs), applying the microbial preparation prepared in the embodiment 3 and the comparative examples 1-2 into the soil, and applying water to the soil to keep the water content of the soil to be 18-25%; after 30 days of application of the microbial formulation; mortality of nematodes and grubs was recorded and the results are reported in table 2.
TABLE 2
| Nematode mortality/% | Grub mortality/% | |
| Example 3 | 98 | 97 |
| Comparative example 1 | 71 | 72 |
| Comparative example 2 | 82 | 79 |
As can be seen from table 2, the insecticidal effects of comparative example 1 and comparative example 2 on nematodes and grubs are inferior to those of example 3, and the difference between comparative example 1 and example 3 is obvious, which indicates that the intermediate layer peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder have certain insecticidal effects; in contrast, comparative example 2 was lower than example 3, and the analysis was probably due to the fact that the microbial preparation prepared in comparative example 2 was inferior in water absorption and low in spore germination rate in a shorter insecticidal time (30 days), so that the insecticidal effect was lowered.
Field test
The prevention and treatment effects of the microbial preparations prepared in the embodiment 3 and the comparative example 1 on peanut root knot nematode and grub diseases and the influence on the growth condition of the peanuts are explored;
selecting a land block for planting peanuts for years, which is easy to generate root-knot nematodes and grubs, of the peanuts planted for a long time as a test land block;
test crop: peanut; variety: flower cultivation 16; sowing: sowing in year 2020 and 2, harvesting: 8 months and 26 days 2020;
the cultivation method comprises the following steps: mulching;
test site: geng Dantun village (yellow river farm) in muu county in Henan province;
selecting land block soil types: wet soil; texture: sand soil;
basic nutrients: organic matter: 9.2g/kg, total nitrogen: 0.67g/kg, quick-acting phosphorus: 8.2mg/kg, quick-acting potassium: 62.6mg/kg;
irrigation and drainage conditions: the land form is flat, and the irrigation and drainage conditions are good;
fertilizing in a test field: 45kg of special fertilizer (20-10-10) is applied to the bottom of each mu, and additional fertilizer is not applied in the growing period.
Test treatment: the test plots are divided into a control group D1 and test groups S1 and S2; when land preparation is carried out on plots before peanut sowing in 5 months, only 45kg of special peanut fertilizer is applied to plots in a control group as base fertilizer, and the microbial preparations prepared in the embodiment 3 and the comparative example 1 of the invention (5 kg are applied to each mu of bottom) are sequentially applied to plots S1 and S2 in test groups except the base fertilizer; 3 groups were repeated for each treatment; planting peanuts in the land parcels of the control group and the test group, carrying out the same field management in the control group and the test group, sampling after the peanuts are sowed for 40 days, respectively counting and recording the density of the mouths of the nematodes and the grubs, calculating an average value (table 3), sampling again after the peanuts are sowed for 80 days, and counting and recording the density of the mouths of the nematodes and the grubs; and the average value (table 3) was calculated, and the growth of peanuts in each treated plot (shown in fig. 1) was recorded, and the results of the peanuts and the yield were statistically recorded at the time of harvesting the peanuts, and the results are shown in fig. 2 and table 3.
TABLE 3 Table 3
As can be seen from the data in table 3, the microbial preparation prepared in example 3 of the present invention shows excellent effects on prevention and control of peanut root-knot nematodes and grubs, and compared with the control group, peanut yield is also significantly improved.
Compared with example 3, the control effect of the comparative example 1 on the peanut root-knot nematodes and the grubs is reduced, and compared with example 3, the comparative example 1 does not contain an intermediate layer consisting of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, and the analysis reasons are probably that the intermediate layer peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in the microbial agent have certain insect repellent effect and have synergistic insect resistance with the microbial agent; however, as the peanut growth time is prolonged, the control effect of comparative example 1 tends to decrease; the plant diseases and insect pests of the peanut root-knot nematode and the grubs rebound; the analysis reasons may be that the microbial agent provides ideal constant environment for the microbial agent at the initial stage of application, part of spores lose activity along with the extension of time, the germination rate of the spores is reduced, and the pest control effect of the microbial agent is reduced.
The above embodiments are merely preferred embodiments of the present invention, the protection scope of the present invention is not limited thereto, and any simple changes or equivalent alternatives of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention disclosed herein fall within the protection scope of the present invention.
Claims (10)
1. The microbial preparation for preventing and controlling the root-knot nematodes and the grubs is characterized by comprising a core layer, an intermediate layer and a coating layer; the core layer comprises paecilomyces lilacinus spore powder and beauveria bassiana spore powder; vegetable oil; bacterial cellulose; silk fibroin; xanthan gum; sucrose esters of fatty acids; the middle layer consists of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder; the coating layer consists of sodium alginate and gluten protein.
2. The microbial preparation for preventing and controlling root-knot nematodes and grubs as claimed in claim 1, wherein the weight ratio of the raw materials in the core layer is: 10-14 parts of paecilomyces lilacinus spore powder and 10-14 parts of beauveria bassiana spore powder; 20-28 parts of vegetable oil; 1-5 parts of bacterial cellulose; 0.5-1 part of silk fibroin, 1-1.4 parts of xanthan gum and 1-1.5 parts of sucrose fatty acid ester.
3. The microbial preparation for preventing and controlling root-knot nematodes and grubs as claimed in claim 1, wherein the weight ratio of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder in the middle layer is 1:1:1:1.
4. the microbial preparation for preventing and controlling root-knot nematodes and grubs according to claim 1, wherein the particle size of the peach leaf powder, the mulberry leaf powder, the pharbitis seed powder and the lotus leaf powder is 100-300 meshes.
5. The microbial preparation for preventing and controlling root-knot nematodes and grubs according to claim 1, wherein the weight ratio of sodium alginate to gluten protein in the coating is 1:0.01-0.05.
6. A method for preparing the microbial preparation for preventing and controlling root-knot nematodes and grubs according to claim 1, which is characterized by comprising the following steps:
(1) Preparing a core layer solution: dispersing paecilomyces lilacinus spore powder and beauveria bassiana spore powder in vegetable oil; adding sucrose fatty acid ester, stirring uniformly, sequentially adding bacterial cellulose, silk fibroin and xanthan gum, stirring uniformly, and obtaining emulsion; injecting the emulsion into a pill dropping machine, and dripping into condensed liquid to obtain a core layer;
(2) Firstly removing condensed liquid on the surface of a core layer, then placing the core layer in mixed powder of peach leaf powder, mulberry leaf powder, pharbitis seed powder and lotus leaf powder, rolling, spraying sodium alginate/gluten protein mixed solution on the surface, placing in a cross-linking agent solution, and cross-linking, solidifying and drying to obtain the microbial preparation.
7. The method for preparing a microbial preparation for preventing and controlling root-knot nematodes and grubs as claimed in claim 6, wherein said condensed liquid is methyl silicone oil.
8. The method for preparing a microbial preparation for controlling both root-knot nematodes and grubs according to claim 6, wherein the cross-linking agent solution is sodium chloride solution.
9. The method for preparing a microbial preparation for controlling both root-knot nematodes and grubs according to claim 6, wherein the mass concentration of sodium alginate in the sodium alginate/gluten protein mixed solution is 2.5wt%.
10. The microbial preparation for preventing and controlling root-knot nematodes and grubs according to any one of claims 1 to 5, for preventing and controlling root-knot nematodes and/or grubs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211580921.0A CN116098150A (en) | 2022-12-07 | 2022-12-07 | Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211580921.0A CN116098150A (en) | 2022-12-07 | 2022-12-07 | Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116098150A true CN116098150A (en) | 2023-05-12 |
Family
ID=86257027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211580921.0A Pending CN116098150A (en) | 2022-12-07 | 2022-12-07 | Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116098150A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101418264A (en) * | 2008-12-04 | 2009-04-29 | 中国科学院微生物研究所 | Paecilomyces lilacinus and application |
| CN101564047A (en) * | 2009-05-27 | 2009-10-28 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN107258823A (en) * | 2017-06-14 | 2017-10-20 | 齐鲁工业大学 | A kind of beauveria bassiana granular formulation preparation method and applications of control of grubs |
| CN107950605A (en) * | 2017-11-30 | 2018-04-24 | 钟山佳欣果蔬有限责任公司 | The microcapsule formulation of citrus orchard pest control |
| CN110037057A (en) * | 2019-05-28 | 2019-07-23 | 河南农业大学 | A kind of broad-spectrum micro-organisms pesticide and preparation method thereof |
| CN112111411A (en) * | 2020-09-27 | 2020-12-22 | 江西华威科技有限公司 | Environment-responsive paecilomyces lilacinus micro-bead and preparation method thereof |
| CN114606156A (en) * | 2022-02-24 | 2022-06-10 | 赤天化科技集团有限公司 | Multifunctional compound microbial agent and preparation method and application thereof |
-
2022
- 2022-12-07 CN CN202211580921.0A patent/CN116098150A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101418264A (en) * | 2008-12-04 | 2009-04-29 | 中国科学院微生物研究所 | Paecilomyces lilacinus and application |
| CN101564047A (en) * | 2009-05-27 | 2009-10-28 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN107258823A (en) * | 2017-06-14 | 2017-10-20 | 齐鲁工业大学 | A kind of beauveria bassiana granular formulation preparation method and applications of control of grubs |
| CN107950605A (en) * | 2017-11-30 | 2018-04-24 | 钟山佳欣果蔬有限责任公司 | The microcapsule formulation of citrus orchard pest control |
| CN110037057A (en) * | 2019-05-28 | 2019-07-23 | 河南农业大学 | A kind of broad-spectrum micro-organisms pesticide and preparation method thereof |
| CN112111411A (en) * | 2020-09-27 | 2020-12-22 | 江西华威科技有限公司 | Environment-responsive paecilomyces lilacinus micro-bead and preparation method thereof |
| CN114606156A (en) * | 2022-02-24 | 2022-06-10 | 赤天化科技集团有限公司 | Multifunctional compound microbial agent and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108432596B (en) | Disease and insect resistant culture medium based on agricultural wastes and preparation method | |
| CN103271095B (en) | Composite microbial agent for preventing and treating fruit tree replant disease and disease prevention method thereof | |
| CN105541512A (en) | Ecological bacterial fertilizer and natural farming ecological planting method | |
| CN105145094A (en) | Integrated ecological tomato cultivation method for reducing pest and disease damage and improving fruit quality | |
| CN111328655B (en) | Wheat-spinach-corn-green manure intercropping planting and cultivating method | |
| CN105340549A (en) | High-yield cultivation method for Jinxiang garlic | |
| CN107567989A (en) | A kind of implantation methods of high yield fruit corn | |
| WO2019015659A1 (en) | Coated controlled-release insecticide dedicated to peanuts and preparation method thereof | |
| CN106576807A (en) | Planting method for promoting peanut growing and fruiting | |
| CN105519389A (en) | Method for preventing diseases and insect pests of allium fistulosum | |
| CN112940969A (en) | Novel microbial agent and soybean planting method | |
| CN112690180A (en) | Efficient pepper substrate seedling culture method | |
| CN116998369A (en) | Ecological control method for controlling field pests through cnidium | |
| CN103960241A (en) | Pesticide composition and method for curing cruciferae crop clubroot | |
| CN106717740A (en) | A kind of paddy rice planting method | |
| CN110604020A (en) | Planting method for pollution-free rice in saline-alkali soil | |
| CN107318451A (en) | Sorghum two is than empty many plants of planting culture methods | |
| CN116098150A (en) | Microbial preparation for preventing and treating root knot nematode and grubs as well as preparation method and application thereof | |
| CN110122502B (en) | Seed coating composition and preparation method and application thereof | |
| CN107711872B (en) | Method for preventing and controlling underground pests in returning wheat straw and corn straw to field | |
| CN113215041A (en) | Microbial agent and planting method for promoting growth of sesame | |
| CN102301875A (en) | Degerming and deinsectization method for solving continuously cropped dead seedlings of Allium chinense | |
| CN110999707A (en) | Crop interplanting method for preventing Indian Miyaya fasciata aphid pests in apple forest | |
| CN105165180A (en) | Preparation method for superstrong multifunctional plant seeds | |
| CN105145196A (en) | Tomato canker integration control method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |