CN116035992A

CN116035992A - Composition with functions of growing and fixing hair, preparation method and application

Info

Publication number: CN116035992A
Application number: CN202310078305.3A
Authority: CN
Inventors: 韩鹤友; 程一林; 程晓紊; 孙双鸽; 陈顺亚
Original assignee: Jiangsu Xiehe Transformation Medical Research Institute Co ltd
Current assignee: Jiangsu Xiehe Transformation Medical Research Institute Co ltd
Priority date: 2023-01-12
Filing date: 2023-01-12
Publication date: 2023-05-02
Also published as: JP2024099465A

Abstract

The invention discloses a composition with functions of growing and fixing hair, a preparation method and application, and belongs to the technical field of growing and fixing hair. The composition comprises the following raw materials in parts by weight: the main components comprise 1020 to 3050 parts of protein and 22.4 to 140 parts of nutrition enhancer; the functional components comprise 20-200 parts of powdered plant sterol ester, 100-500 parts of millet extract, 100-500 parts of rhizoma polygonati powder, 100-400 parts of mulberry powder and 100-500 parts of Aronia melanocarpa fruit powder; auxiliary ingredients, including 200-1800 parts of flavoring agent. The composition has no toxic or side effect, can promote metabolism and proliferation of hair follicle cells, can effectively promote formation of hair follicle melanin, shortens the time of hair follicle entering into growing period from resting period and growing hair, and can strengthen the tightness of hair in scalp, thereby achieving the effects of preventing hair loss and fixing hair.

Description

Composition with functions of growing and fixing hair, preparation method and application

Technical Field

The invention belongs to the technical field of hair growth and hair fixation, and particularly relates to a composition with hair growth and hair fixation functions, a preparation method and application.

Background

Statistics show that alopecia has become increasingly prevalent and a trend towards low age.

Products with functions of growing and fixing hair are declared to be more in the market, but the products are not ideal in the aspect of growing and fixing hair; most hair-growing products are washing and caring products, and other products are few, however, the washing and caring products are required to have a cleaning function, and chemical components such as a surfactant and a conditioner which can damage hair are inevitably added, so that a composition with good hair-growing and hair-fixing effects is needed.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a composition with functions of generating and fixing hair, a preparation method and application.

In order to achieve the above purpose, the present invention provides the following technical solutions:

one of the technical schemes of the invention is as follows: the composition with the hair growth and fixation functions comprises the following raw materials in parts by weight:

further, the proteins include small molecule peptides and amino acids; the small molecular peptide comprises 500-1500 parts by weight of bovine bone collagen peptide and 500-1500 parts by weight of hydrolyzed collagen peptide; the amino acid comprises 20-50 parts of taurine in parts by weight.

Further, the nutrition enhancer is a vitamin enhancer and a mineral enhancer.

Further, the vitamin reinforcer comprises 0.2 to 10 parts of vitamin A, 10 to 50 parts of vitamin C, 1 to 10 parts of vitamin E and 10 to 50 parts of yeast powder in parts by weight; the mineral fortifier comprises, by weight, 1-10 parts of ferrous fumarate and 0.2-10 parts of zinc oxide.

Further, the powdered plant sterol ester is beta-sitosterol ester with a chemical formula of C ₄₇ O ₂ H ₈₀ 。

Further, the preparation method of the millet extract comprises the following steps: pulverizing semen Setariae, extracting with water, concentrating the extractive solution, and lyophilizing to obtain semen Setariae extract.

Further, the flavoring agent comprises 100-500 parts by weight of erythritol and 100-500 parts by weight of grapefruit powder.

The second technical scheme of the invention is as follows: the preparation method of the composition with the hair growth and fixation functions comprises the following steps:

(1) Weighing the raw materials according to the weight parts, and sieving for standby;

(2) Mixing the nutrition enhancer and the flavoring agent weighed in the step (1) to obtain a mixed raw material A;

(3) Placing the protein and the functional components weighed in the step (1) into a granulator for stirring, granulating by taking alcohol as an adhesive, drying by a fluidized bed, and granulating by a granulator to obtain a mixed raw material B;

(4) And (3) mixing the mixed raw material A obtained in the step (2) with the mixed raw material B obtained in the step (3), and sieving to obtain the composition with the hair growth and hair strengthening functions.

Further, in the step (1), the sieving is performed by a 40-100 mesh sieve.

Further, in step (2), the mixing is an equal incremental mixing.

Further, in the step (3), the stirring revolution is 200-500 revolutions per minute; the alcohol consumption is 280-480 parts by mass of 70-95% alcohol sprayed within 2-10 min; the drying temperature of the fluidized bed is 51-58 ℃ and the drying time is 25-35 min; the granulator is a 20-mesh granulator.

Further, in the step (4), the sieving is performed by a 40-100 mesh sieve.

The third technical scheme of the invention: the composition with the functions of promoting hair growth and fixing hair is applied to the aspects of promoting hair growth and fixing hair.

Compared with the prior art, the invention has the following beneficial effects:

(1) The composition with the functions of growing and fixing hair prepared by the invention can shorten the time of hair follicle from resting stage to growing stage, can accelerate the time of growing hair, and has remarkable promoting effect on hair growth;

(2) The composition with the functions of generating and fixing hair prepared by the invention has no toxic or side effect, and can effectively promote hair follicle regeneration;

(3) The invention adopts the fluidized bed granulation instead of the oven drying, and has the advantages of relatively oven drying, uniform flow state, even drying, less damage to the surface of the material and more loose powder; the heat efficiency is high, the energy-saving effect is good, and the energy can be saved by 30 to 60 percent compared with the drying of an oven.

The added hydrolyzed collagen peptide belongs to a necessary composition substance of human skin, can effectively ensure skin moisture, increase skin firmness, and the most main protein in hair is keratin, so that the collagen can support and increase in-vivo hair growth proteins. This can help strengthen the hair, promote hair growth, and prevent hair loss;

in addition, the two collagen peptides are selected because one collagen peptide is extracted from animal skin, and the other collagen peptide is derived from bone, and the amino acid components and peptide fragments are different and can be mutually complemented;

the taurine added by the invention not only has the effect of repairing damaged hair, but also has the effects of increasing the generation of hair growth factors and promoting hair growth. Taurine increases the production of the growth factor IGF-1, which acts on the human hair cycle, lengthens the anagen phase, and produces thicker and stronger hair;

the added multivitamins can increase scalp nutrition, promote hair follicle cell metabolism and further promote hair growth;

the added yeast powder contains vitamin H and nicotinic acid, wherein the vitamin H is also called biotin, is a necessary nutrient for maintaining natural growth and development of a human body and normal human body functional health, can directly regulate and control metabolism of hair follicle cells, and can also indirectly influence hair follicle development by influencing feed intake or metabolism;

in addition, the nicotinic acid contained in the yeast powder is also an indispensable nutrient component in human body, plays an important role in promoting the normal growth and development of the human body, can promote iron absorption and the generation of blood cells, and maintains the normal function of skin;

ferrous fumarate is added, and the iron element provides oxygen for each cell of the body, including cells in scalp and hair follicles;

the zinc oxide is added, and zinc is an auxiliary factor of various enzymes in the body or an important component of antioxidant enzymes in the body, so that the activity of blood cells can be enhanced, and the zinc oxide is a trace element necessary for hair growth and keratin synthesis;

the powdered plant sterol ester added by the invention is prepared from plant sterol and fatty acid through esterification reaction or transesterification reaction. Sterol esters are capable of being converted to sterols and fatty acids in the human body, so their physiological functions include those possessed by both plant sterols and fatty acids. The plant sterol ester is easier to be absorbed by human body, the use efficiency is far higher than that of common plant sterols, and the alopecia can be prevented by inhibiting the generation of androgens;

the millet extract added by the invention is a rare oil extracted from millet, in particular to a millet extract (chemical formula: C) ₃₁ H ₅₂ O), the rare oil acts on the root of hair follicle, can accelerate the metabolism and proliferation of cells, keep the hair growing, nourish and repair damaged scalp, create good basic conditions for healthy hair, and have anti-inflammatory and anti-toxic effects;

the added rhizoma polygonati powder has the effects of returning kidney meridian, benefiting essence and filling marrow, and has good effects of treating kidney deficiency type alopecia and the like;

the mulberry powder added by the invention has the effects of tonifying kidney and replenishing essence and Wu Fasheng hair, and is suitable for the symptoms of premature senility, premature graying of beard and hair, hair loss, alopecia areata and hair withering; mulberry is a material in a medicine and food homologous catalog, and has the effect of treating premature graying of beard and hair according to pharmacopoeia records; mulberry is rich in procyanidins, and procyanidins can induce telogen hair follicle conversion to anagen phase by activating stem cells of hair follicle Long Tubu, so as to promote hair regeneration;

the Aronia melanocarpa fruit powder added by the invention is rich in various vitamins, antioxidant components and mineral elements, and has the effect of improving the blood circulation of the head.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a graph showing the change in back hair of mice to which sample 3# was administered;

FIG. 2 is a graph showing the change in back hair of mice dosed with low dose samples;

FIG. 3 is a graph showing the change in back hair of mice dosed with high doses of the samples;

FIG. 4 is a graph showing the change in back hair of mice dosed with positive control samples;

FIG. 5 is a graph showing the change in back hair of mice dosed with a negative control sample;

FIG. 6 is a statistical plot of the number of microscopic new hair follicles for dosing sample 3#, low dose sample, high dose sample, positive control sample, and negative control sample;

FIG. 7 shows HE staining patterns of the administered sample 3#, wherein (a) shows HE staining patterns of 7-day mouse skin histology, (b) shows HE staining patterns of 14-day mouse skin histology, (c) shows HE staining patterns of 28-day mouse skin histology, and (d) shows HE staining patterns of 56-day mouse skin histology;

FIG. 8 is a graph of HE staining of a low dose sample administered, wherein (a) is a graph of HE staining of 7 day mouse skin histology, (b) is a graph of HE staining of 14 day mouse skin histology, (c) is a graph of HE staining of 28 day mouse skin histology, and (d) is a graph of HE staining of 56 day mouse skin histology;

FIG. 9 is a graph of HE staining of a high dose sample administered, wherein (a) is a graph of HE staining of 7 day mouse skin histology, (b) is a graph of HE staining of 14 day mouse skin histology, (c) is a graph of HE staining of 28 day mouse skin histology, and (d) is a graph of HE staining of 56 day mouse skin histology;

FIG. 10 is a graph of HE staining of a positive control sample administered, wherein (a) is a graph of HE staining of 7-day mouse skin histology, (b) is a graph of HE staining of 14-day mouse skin histology, (c) is a graph of HE staining of 28-day mouse skin histology, and (d) is a graph of HE staining of 56-day mouse skin histology;

FIG. 11 is a graph of HE staining of a negative control sample administered, wherein (a) is a graph of HE staining of 7-day mouse skin histology, (b) is a graph of HE staining of 14-day mouse skin histology, (c) is a graph of HE staining of 28-day mouse skin histology, and (d) is a graph of HE staining of 56-day mouse skin histology.

Detailed Description

Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.

It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.

As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.

The parts in the embodiments of the present invention are parts by weight unless otherwise specified.

In the following examples, the powdered phytosterol esters were prepared according to patent CN 201410445798.0;

in the following examples, the preparation method of the millet extract comprises the following steps:

1) Drying semen Setariae in a hot air drying oven at 37deg.C for 24 hr, pulverizing, and sieving with 80 mesh sieve;

2) Vortex extracting the millet sieved in the step 1) with water at 50 ℃ for 1h according to the feed-liquid ratio of 1:10, filtering, repeating for two times, and respectively collecting filtrate and filter residues;

3) Concentrating the filtrate obtained in the step 2) until the solid content is 25-35% to obtain a concentrated solution;

4) Freeze-drying the concentrated solution obtained in the step 3) to obtain the millet extract, wherein the parameters of freeze-drying are that the water content is kept below 6%;

the mulberry powder, the Aronia melanocarpa fruit powder and the rhizoma polygonati powder are commercially available in the following examples, wherein the content of procyanidine in the mulberry powder, the Aronia melanocarpa fruit powder is 10%, and the content of yellow extract polysaccharide in the rhizoma polygonati powder is 15%;

the bovine collagen peptide and the hydrolyzed collagen peptide in the following examples are commercially available, and are food raw materials, and the protein content is 95%;

the vitamin A in the following examples is commercially available and is a food grade additive with an active substance content of 32.5 ten thousand IU/g;

the vitamin C in the following examples is commercially available as a food grade additive with an active substance content of 97%;

the taurine and ferrous fumarate in the following examples are commercially available and are food grade additives, and the content of effective substances is 99%;

the vitamin E in the following examples is commercially available and is a food grade additive, and the content of the active ingredients is 50%;

the yeast powder described in the following examples is commercially available, and the active substance niacin content is 40mg/g and biotin content is 300ppm.

In the following examples, the term "equal incremental mixing" refers to: the equal amount is increased to the raw material with the least dosage and the other raw material with the equal amount, the raw materials are uniformly mixed, then the other raw materials with the equal amount after the mixing are added, and the mixing of all the raw materials is completed.

Example 1

(1) Weighing the raw materials according to the weight parts described in the 1# in the table 1, and sieving the raw materials with a 40-mesh sieve for standby;

(2) Incrementally mixing the nutrition enhancer and the flavoring agent weighed in the step (1) in equal amount to obtain a mixed raw material A;

(3) Placing the protein and the functional components weighed in the step (1) into a granulator for stirring, granulating by taking alcohol as an adhesive, drying by a fluidized bed, and granulating by a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 200 revolutions per minute, the alcohol consumption is 280 parts of 70% alcohol sprayed within 2 minutes, the drying temperature of the fluidized bed is 51 ℃, and the time is 25 minutes;

(4) Mixing the mixed raw material A obtained in the step (2) with the mixed raw material B obtained in the step (3), and sieving with a 40-mesh sieve to obtain the composition with the functions of generating and fixing hair.

Example 2

(1) Weighing the raw materials according to the weight parts described in the No. 2 in the table 1, and sieving the raw materials with a 100-mesh sieve for standby;

(3) Placing the protein and the functional components weighed in the step (1) into a granulator for stirring, granulating by taking alcohol as an adhesive, drying by a fluidized bed, and granulating by a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 500 revolutions per minute, 480 parts of 95% alcohol is sprayed within 10 minutes, and the drying temperature of the fluidized bed is 58 ℃ for 35 minutes;

(4) Mixing the mixed raw material A obtained in the step (2) with the mixed raw material B obtained in the step (3), and sieving with a 100-mesh sieve to obtain the composition with the functions of generating and fixing hair.

Example 3

(1) Weighing the raw materials according to the weight parts described in 3# in table 1, and sieving with a 60-mesh sieve for standby;

(3) Placing the protein and the functional components weighed in the step (1) into a granulator for stirring, granulating by taking alcohol as an adhesive, drying by a fluidized bed, and granulating by a 20-mesh granulator to obtain a mixed raw material B, wherein the stirring speed is 350 r/min, 300 parts of 85% alcohol is sprayed within 5.5min, and the drying temperature of the fluidized bed is 55 ℃ for 30min;

(4) Mixing the mixed raw material A obtained in the step (2) with the mixed raw material B obtained in the step (3), and sieving with a 60-mesh sieve to obtain the composition with the functions of generating and fixing hair.

Table 1 amounts of raw materials

Comparative example 1

The procedure of example 3 was repeated except that the raw materials were weighed in the parts by weight shown in Table 2, and sieved through a 60-mesh sieve in step (1).

Comparative example 2

The difference in the procedure of example 3 is that the raw materials are weighed in the parts by weight shown in Table 2# 5 and sieved with a 60 mesh sieve for use in the procedure of step (1).

Comparative example 3

The difference in the procedure of example 3 is that the raw materials in the step (1) are weighed in the parts by weight shown in Table 2# 6 and sieved with a 60 mesh sieve for use.

Comparative example 4

The procedure of example 3 was repeated except that the raw materials were weighed in the parts by weight shown in Table 2 No. 7 in step (1), and sieved with a 60-mesh sieve.

Comparative example 5

The difference in the procedure of example 3 is that the raw materials in the step (1) are weighed in parts by weight as shown in Table 2# 8 and sieved with a 60 mesh sieve for use.

Comparative example 6

The difference in the procedure of example 3 is that the raw materials in the step (1) are weighed according to the parts by weight shown in 9# in Table 2 and sieved with a 60 mesh sieve for use.

Comparative example 7

The same procedure as in example 3 was repeated except that the raw materials were weighed in the parts by weight shown in Table 2 at 10# and sieved through a 60 mesh sieve for use in step (1).

Comparative example 8

The procedure of example 3 was repeated except that the raw materials were weighed in the parts by weight shown in 11# in Table 2 in step (1), and sieved with a 60-mesh sieve.

Comparative example 9

The difference in the procedure of example 3 is that the raw materials are weighed in the parts by weight shown in Table 2# 12 and sieved with a 60 mesh sieve for use in the procedure of step (1).

Comparative example 10

The difference is that the black bean powder is used to replace the mulberry powder in the step (1) as in example 3.

TABLE 2 amounts of raw materials

Effect verification

1. Prescription effect verification

Selection of (one) subject

Selecting qualified subjects according to the inclusion and exclusion criteria;

1. criteria for inclusion

(1) 25-45 years old, healthy men or women;

the length of the hair is between 5 and 40 cm;

(2) The hair loss is excessive and the hair is slightly sparse, and the hair loss count is more than 10 according to a 60-time comb method, and the hair loss count is still more than 10 after a 2-week preparation period;

(3) No special hair-dressing treatment such as hair dyeing, hair waving and shaping is performed within 1 month;

2. exclusion criteria

(1) Pregnant or lactating women, or those who have a pregnancy preparation plan recently;

(2) Patients suffering from mental or psychological disorders; or with long-term sleep, mood control disorders;

(3) Cosmetic with hair loss preventing effect or other products with such effect or hair growth promoting effect are used in about 3 months;

(4) Any drugs affecting hair growth are taken or topically applied in the last 6 months;

3. subject restriction

(1) During the screening and testing period of the subjects, the hair cannot be washed within 48+/-4 hours before each visit, the time of not washing the hair before each visit is basically consistent, and the hair cannot be combed by oneself on the day of the visit;

(2) No haircut was performed during the trial period for 2 weeks prior to each visit assessment;

(3) During the test, any hair care and hairdressing treatment measures cannot be performed, and any treatments for preventing alopecia and generating hair cannot be accepted;

(4) The original living habit is required to be kept during the test, so that the large emotion fluctuation is avoided;

(II) test substance

Test sample: the compositions prepared in examples 1 to 3 and comparative examples 1 to 9;

the using method comprises the following steps: randomly distributing test samples to the test subjects, and guiding the sample use of the test subjects to ensure that the test subjects correctly use the test objects in a test period, wherein the test objects are used for 12 weeks, and the test subjects are required to record the use time and any discomfort and adverse reaction symptoms in the use process during the test period;

(III) test Equipment

Comb: the comb teeth have moderate density (the tooth space is 0.9-1.1 mm), the length of the comb teeth is 2.0-3.0 cm, the length of the comb is not less than 10cm (no comb handle is contained), the comb with the same specification and material is required to be used in the whole test process, and the related requirements in the medical institution disinfection technical Specification (WS-T367-2012) are referred to for disinfection after each use;

(IV) test procedure

Subjects were recruited as required, and the number of hair loss was counted and recorded using 60 comb hair treatments;

qualified subjects underwent a 2-week preparation period using 0.9% sodium chloride solution; after the preparation period is finished, performing a hair combing method for 60 times again, and entering a formal test for the people with the hair loss count still greater than 10;

subjects enrolled in the formal trial were grouped into 12 groups according to a hierarchical randomization method, ensuring that factors that might affect the outcome of the trial, for example: balance of sex, age, hair length, severity of hair loss, etc.;

performing evaluation of hair base values prior to product use, including hair loss count, record; the same evaluation and test was again performed after 4 weeks, 8 weeks, 12 weeks after the use of the product;

(V) alopecia count: subjects were combed with their hair by a trained staff member at each visit using a 60-pass combing method, the hair loss counted, and recorded.

(sixth) test results

The result of verification of the prescription effect is shown in table 3;

table 3 results of prescription Effect verification

(seventh) conclusion

As can be seen from the data of table 3, the difference in the hair loss count after 12 weeks (hair loss count before product use-hair loss count after 12 weeks of product use) of examples 1 to 3 was higher than that of comparative examples 1 to 10, and therefore, the hair loss preventing efficacy of examples 1 to 3 was better than that of comparative examples 1 to 10; in examples 1 to 3, the anti-hair loss effect of example 3 was superior to examples 1 and 2.

2. Dose effect verification

Experimental materials and methods

1. Grouping mice:

50C 57BL/6J mice (SPF grade) with the age of 38-40 d and the weight of 15-20 g (provided by experimental animal center of southern medical university) are randomly divided into 5 groups after 3 days of adaptive feeding, 10 mice in each group are shaved at 24 hours before dosing, and the hair of the back of the mice is induced to enter the growing period from the resting period;

2. sample configuration:

1.54g of the composition prepared in example 3 was completely dissolved in 20mL of water to obtain sample # 3;

0.77g of the composition prepared in example 3 was completely dissolved in 20mL of water to obtain a low dose sample;

3.08g of the composition prepared in example 3 was completely dissolved in 20mL of water to obtain a high dose sample;

preparing finasteride solid into a finasteride solution with the concentration of 0.2%, so as to obtain a positive control sample;

preparing sodium chloride solid into 0.9% sodium chloride solution to obtain a negative control sample;

3. administration of drugs

Sample 3# and four control samples were administered to 5 mice 1 time per day, 0.2mL each time, for 8 weeks.

(II) results of experiments

1. Weight change in mice

The body weight of the mice is weighed and recorded every 7d in the detection process, the average value is expressed as a mean value plus or minus standard deviation, and the specific results are shown in table 4;

TABLE 4 weight variation of mice (Unit: g)

From the above table data, it can be found that the body weight change trend of the 5 groups of mice is basically consistent in the whole detection process, and the mice have no abnormality.

2. Time required for skin changes and hair growth in mice

The skin and hair growth conditions of the dehairing areas of each group of mice were observed every day, the specific hair growth conditions are shown in fig. 1 to 5 (shooting is performed every 7 d), the time for changing the skin color of each mouse dehairing area from pink to black and the time for starting growing hair are recorded, the average value is expressed as a mean value + -standard deviation, and the specific results are shown in table 5;

from the above graph, sample 3# showed that 2 mice had grayed skin and 2 mice had grown hair in D14, and 4 mice were found to have significantly grown hair in D21, with 1 mouse having hair grown in the 60% area of the back and 3 mice having all grown back;

the low dose samples showed greying of the skin of 1 mouse at D14 and found 5 mice growing hair at D21, 2 mice growing hair in the 70% area of the back and 2 mice growing hair in the 80% area of the back;

the high dose samples showed 5 hair growth on the backs of the 5 mice in D14 and 5 mice on D21, with 3 mice on 90% of the back and 2 mice on the whole;

the positive control samples showed 4 mice with greying skin, one with a pronounced hair growth on D14 and 6 mice with a 90% area on the back were found to have hair growth on D21.

The negative control samples showed graying of the skin in 2 mice at D14, 3 mice with hair growth in the 70% area of the back and 2 mice with hair growth in the 90% area of the back were found at D21.

TABLE 5 skin changes and time required for growing hair in mice (unit: d)

From the above table data, it can be seen that sample 3#, high dose sample, and positive control sample shortened the time to enter the anagen phase and grow hair to a different extent than the negative control sample; among them, sample 3# more significantly shortens the time for the hair follicle to enter anagen phase from telogen phase and grow out of hair.

3 Back skin histological examination of mice

Humane sacrifice after 24h of administration on day 7, day 14, day 28, day 56, 2 per sacrifice, skin specimens were taken at the same site in the shaved area, fixed, conventional flaking, HE staining, and the number of microscopic new hair follicles expressed as an average, with specific results shown in tables 6-9 and fig. 6;

table 67 skin histology index (Unit: personal) of mice

Table 7 skin histology index (Unit: personal) of 14 day mice

Table 8 skin histology index (Unit: personal) of mice over 28 days

Table 9 skin histology index (Unit: personal) of 56 days mice

HE staining patterns of sample 3#, low dose sample, high dose sample, positive control sample, and negative control sample are shown in fig. 7 to 11:

FIG. 7 is a graph of HE staining of sample 3# wherein (a) is a graph of HE staining of 7 day mouse skin histology, (b) is a graph of HE staining of 14 day mouse skin histology, (c) is a graph of HE staining of 28 day mouse skin histology, and (d) is a graph of HE staining of 56 day mouse skin histology;

FIG. 8 is a plot of HE staining of low dose samples, wherein (a) is a plot of HE staining of 7 day mouse skin histology, (b) is a plot of HE staining of 14 day mouse skin histology, (c) is a plot of HE staining of 28 day mouse skin histology, and (d) is a plot of HE staining of 56 day mouse skin histology;

FIG. 9 is a plot of HE staining of high dose samples, wherein (a) is a plot of HE staining of 7 day mouse skin histology, (b) is a plot of HE staining of 14 day mouse skin histology, (c) is a plot of HE staining of 28 day mouse skin histology, and (d) is a plot of HE staining of 56 day mouse skin histology;

FIG. 10 is a graph of HE staining of positive control samples, wherein (a) is a graph of HE staining of 7-day mouse skin histology, (b) is a graph of HE staining of 14-day mouse skin histology, (c) is a graph of HE staining of 28-day mouse skin histology, and (d) is a graph of HE staining of 56-day mouse skin histology;

FIG. 11 is a graph of HE staining of a negative control sample, wherein (a) is a graph of HE staining of 7-day mouse skin histology, (b) is a graph of HE staining of 14-day mouse skin histology, (c) is a graph of HE staining of 28-day mouse skin histology, and (d) is a graph of HE staining of 56-day mouse skin histology.

As can be seen from figures 6 to 11,

the negative control group is found to be in a descending trend in the whole period of the experiment through the skin histological examination of the back of the mice, and the new hair follicle number reaches a peak value in the D7; whereas the number of new hair follicles in the 3# high dose group D14 reached a maximum value, to a number of new hair follicles in the D28 group of 0; the number D7 of the new hair follicles in the positive control group reaches a peak value, and the whole experimental process shows a descending trend.

The number of new hair follicles in the negative control group is lower than that in the 3# and positive control groups in both the D7 and the D14, and the number of new hair follicles in the D28 negative control group is 0;

the number of new hair follicles in the low dose group is basically consistent with that in the negative control group, and the number of new hair follicles in the D7 and D14 is higher than that in the 3# and negative control groups;

the number of new hair follicles of 3# is obviously more than that of the new hair follicles of D7, D14 and D28 than that of the negative control group, the number of new hair follicles of D7 is 48.40 percent higher than that of the negative control group, the number of new hair follicles of D14 reaches the peak value, and the number of new hair follicles of D14 is 218.00 percent higher than that of the negative control group;

the number of new hair follicles in the high dose group is higher than that in the negative control group in D7 and D14, the number of new hair follicles in the D7 is 19.36 percent higher than that in the negative control group, the number of new hair follicles in the D14 is 32.15 percent higher than that in the negative control group, and the number of new hair follicles in the D28 and the number of new hair follicles in the negative control group are both 0;

the positive control group D7 reached a peak value, and showed a downward trend after D14, and the number of new hair follicles in the D28 group and the number of new hair follicles in the negative control group were both 0.

In conclusion, the composition provided by the invention has no toxic or side effect, can promote metabolism and proliferation of hair follicle cells, promote hair follicle neogenesis, shorten the time of hair follicle from a resting stage to a growing stage, has a remarkable promoting effect on hair growth, and is beneficial to strengthening the tightness of hair in scalp, so that the purpose of preventing hair loss and fixing hair is achieved.

In the foregoing, the protection scope of the present invention is not limited to the preferred embodiments, and any person skilled in the art, within the scope of the present invention, should be covered by the protection scope of the present invention by equally replacing or changing the technical scheme and the inventive concept thereof.

Claims

1. The composition with the functions of promoting hair growth and strengthening hair is characterized by comprising the following raw materials in parts by weight:

2. the composition having a hair growth and hair strengthening function according to claim 1, wherein the protein comprises a small molecule peptide and an amino acid; the small molecular peptide comprises 500-1500 parts by weight of bovine bone collagen peptide and 500-1500 parts by weight of hydrolyzed collagen peptide; the amino acid comprises 20-50 parts of taurine in parts by weight.

3. The composition having a hair growth promoting and hair strengthening function according to claim 1, wherein the nutrition enhancer is a vitamin-based enhancer and a mineral-based enhancer.

4. The composition with hair growth and hair strengthening function according to claim 3, wherein the vitamin enhancer comprises, by weight, 0.2-10 parts of vitamin A, 10-50 parts of vitamin C, 1-10 parts of vitamin E and 10-50 parts of yeast powder; the mineral fortifier comprises, by weight, 1-10 parts of ferrous fumarate and 0.2-10 parts of zinc oxide.

5. The composition having a hair growth and hair strengthening function according to claim 1, wherein the powdery plant sterol ester is β -sitosterol ester.

6. The composition with hair growth and hair strengthening function according to claim 1, wherein the millet extract is prepared by the following steps: pulverizing semen Setariae, extracting with water, concentrating the extractive solution, and lyophilizing to obtain semen Setariae extract.

7. The composition with hair growth and hair strengthening function according to claim 1, wherein the flavoring agent comprises, by weight, 500-1200 parts of erythritol and 300-600 parts of grapefruit powder.

8. A method for preparing the composition having the hair growth and hair fixing function according to any one of claims 1 to 7, comprising the steps of:

9. The method according to claim 8, wherein in the step (1), the sieving is performed by a 40-100 mesh sieve; in the step (2), the mixing is equal incremental mixing; in the step (3), the stirring revolution is 200-500 revolutions per minute; the alcohol consumption is 280-480 parts by mass of 70-95% alcohol sprayed within 2-10 min; the drying temperature of the fluidized bed is 51-58 ℃ and the drying time is 25-35 min; the granulator is a 20-mesh granulator; in the step (4), the sieving is carried out by a sieve with 40-100 meshes.

10. Use of the composition having hair growth and hair-fixing function according to any one of claims 1 to 7 for hair growth and hair-fixing.