CN116004246B - Microbial farmland soil restoration agent and microbial farmland soil restoration method - Google Patents
Microbial farmland soil restoration agent and microbial farmland soil restoration method Download PDFInfo
- Publication number
- CN116004246B CN116004246B CN202310123823.2A CN202310123823A CN116004246B CN 116004246 B CN116004246 B CN 116004246B CN 202310123823 A CN202310123823 A CN 202310123823A CN 116004246 B CN116004246 B CN 116004246B
- Authority
- CN
- China
- Prior art keywords
- soil
- parts
- microbial
- restoration agent
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002689 soil Substances 0.000 title claims abstract description 171
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 72
- 230000000813 microbial effect Effects 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 27
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 25
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 23
- 241000589180 Rhizobium Species 0.000 claims abstract description 22
- 241000190834 Chromatiaceae Species 0.000 claims abstract description 21
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 17
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000008103 glucose Substances 0.000 claims abstract description 17
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 16
- 239000001110 calcium chloride Substances 0.000 claims abstract description 16
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 16
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 16
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 15
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 238000002791 soaking Methods 0.000 claims description 18
- 239000003621 irrigation water Substances 0.000 claims description 13
- 238000004080 punching Methods 0.000 claims description 12
- 238000003971 tillage Methods 0.000 claims description 12
- 239000010902 straw Substances 0.000 claims description 10
- 238000003306 harvesting Methods 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 9
- 239000002985 plastic film Substances 0.000 claims description 9
- 229920006255 plastic film Polymers 0.000 claims description 9
- 238000009331 sowing Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 2
- 230000035699 permeability Effects 0.000 abstract description 12
- 230000035558 fertility Effects 0.000 abstract description 7
- 230000006872 improvement Effects 0.000 abstract description 7
- 230000000052 comparative effect Effects 0.000 description 18
- 239000003513 alkali Substances 0.000 description 13
- 230000000694 effects Effects 0.000 description 11
- 150000003839 salts Chemical class 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 8
- 238000011282 treatment Methods 0.000 description 7
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 244000105624 Arachis hypogaea Species 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 235000020232 peanut Nutrition 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 239000003516 soil conditioner Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention provides a microbial cultivated land soil restoration agent and a microbial cultivated land restoration method, and belongs to the technical field of soil improvement. The microbial farmland soil restoration agent provided by the invention comprises the following raw materials in parts by weight: 4-8 parts of bacillus subtilis, 8-15 parts of purple sulfur bacteria, 10-17 parts of rhizobium, 1-3 parts of trichoderma harzianum, 0.05-0.2 part of proline, 0.1-0.5 part of glucose, 0.4-1.2 parts of potassium dihydrogen phosphate and 0.3-0.6 part of calcium chloride. The invention can effectively improve the hardening phenomenon of the cultivated land soil, improve the permeability and fertility of the soil, reduce the salinization degree of the soil and further improve the crop yield.
Description
Technical Field
The invention belongs to the technical field of soil improvement, and particularly relates to a microbial farmland soil restoration agent and a microbial farmland soil restoration method.
Background
Land resources are the most basic and precious resources in all natural resources, and are important production materials which are not replaced in agricultural production. At present, in the utilization of cultivated land soil, the method for increasing the fertility is mainly to apply fertilizer, and the organic matter content of the soil is reduced due to single bias fertilizer for a long time. The farmland soil is unreasonably cultivated for a long time, the fertility is reduced, the permeability is poor, and hardening and cracking of the soil are caused. Soil hardening causes the reduction of the water absorption, oxygen absorption and nutrient absorption capacity of the soil, and the reduction of the permeability leads to poor root system development of crops and influences the growth and development of the crops.
In addition, salinization of soil can also cause hardening of cultivated land soil. The saline-alkali soil refers to soil with excessive saline-alkali components accumulated on the surface layer, has the characteristics of high salt content and pH value, easy hardening of the surface layer, poor air permeability and water permeability and low nutrient content, and high-concentration salt inhibits normal growth of crops. The saline-alkali soil in China is widely distributed, and the saline-alkali soil becomes a main factor affecting the quality of cultivated land and restricting and jeopardizing the sustainable development of agricultural production.
Thus, there is an urgent need to provide a method for improving saline-alkali soil and improving farmland quality to promote sustainable development of agriculture.
Disclosure of Invention
In view of the above, the invention aims to provide a method for repairing cultivated land soil by microorganisms, which can effectively improve the hardening phenomenon of the cultivated land soil, improve the permeability and fertility of the soil, reduce the salinization degree of the soil and further improve the crop yield.
In order to achieve the above object, the present invention provides the following technical solutions:
The microbial farmland soil restoration agent comprises the following raw materials in parts by weight: 4-8 parts of bacillus subtilis, 8-15 parts of purple sulfur bacteria, 10-17 parts of rhizobium, 1-3 parts of trichoderma harzianum, 0.05-0.2 part of proline, 0.1-0.5 part of glucose, 0.3-0.6 part of potassium dihydrogen phosphate and 0.4-1.2 parts of calcium chloride.
Preferably, the material comprises the following raw materials in parts by weight: 5-6 parts of bacillus subtilis, 10-12 parts of purple sulfur bacteria, 13-15 parts of rhizobium, 1.5-2 parts of trichoderma harzianum, 0.12-0.16 part of proline, 0.2-0.4 part of glucose, 0.4-0.5 part of potassium dihydrogen phosphate and 0.6-1.0 part of calcium chloride.
Preferably, the material comprises the following raw materials in parts by weight: 5 parts of bacillus subtilis, 11 parts of purple sulfur bacteria, 15 parts of rhizobium, 1.5 parts of trichoderma harzianum, 0.15 part of proline, 0.3 part of glucose, 0.45 part of potassium dihydrogen phosphate and 0.8 part of calcium chloride.
Preferably, the bacillus subtilis, the sulfoviolet bacteria, the rhizobium and the trichoderma harzianum are respectively liquid-cultured bacillus subtilis liquid, purple sulfur bacteria liquid, rhizobium liquid and trichoderma harzianum liquid.
Another object of the present invention is to provide a method for restoring cultivated land soil by microorganisms, comprising the steps of:
(1) Weighing the microbial farmland soil restoration agent, and mixing the microbial farmland soil restoration agent with water to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the depth of the holes is 5-10cm, the diameter of the holes is 1-2cm, the interval between the holes is 30-50cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, soaking the field for 18-25 days, and draining water;
(3) 10 days before crop sowing, rotary tillage soil preparation is carried out on the field, the liquid soil restoration agent is applied to soil along with irrigation water, and water is drained after 3-8 hours of field soaking.
Preferably, the liquid soil restoration agent is prepared by mixing microbial cultivated land soil restoration agent and water according to a mass ratio of 1:05-0.8.
Preferably, in the step (2) and the step (3), the soaking field is that the water depth is 1-5cm beyond the soil layer.
Preferably, the application amount of the liquid soil restoration agent in the step (2) is 50-80 kg/mu; the application amount of the liquid soil restoration agent in the step (3) is 100-130 kg/mu.
Preferably, in the step (2), the surface of the field block is covered with a plastic film or plant straw after the field is soaked and drained.
Preferably, the thickness of the plastic film is more than or equal to 0.02mm; the plant straw coverage thickness is 5-10cm.
Compared with the prior art, the invention has the following beneficial effects:
The invention provides a microbial farmland soil restoration agent and a microbial farmland soil restoration method, which can effectively reduce the saline-alkali degree of soil, increase the air permeability and the water permeability of the soil, improve the hardening condition of the soil, and improve the fertility of the farmland soil, thereby improving the crop yield. The method provided by the invention is beneficial to improving the saline-alkali cultivated land condition and promoting the benign development of agriculture.
Detailed Description
The invention provides a microbial farmland soil restoration agent which comprises the following raw materials in parts by weight: 4-8 parts of bacillus subtilis, 8-15 parts of purple sulfur bacteria, 10-17 parts of rhizobium, 1-3 parts of trichoderma harzianum, 0.05-0.2 part of proline, 0.1-0.5 part of glucose, 0.3-0.6 part of potassium dihydrogen phosphate and 0.4-1.2 parts of calcium chloride.
Preferably, the material comprises the following raw materials in parts by weight: 5-6 parts of bacillus subtilis, 10-12 parts of purple sulfur bacteria, 13-15 parts of rhizobium, 1.5-2 parts of trichoderma harzianum, 0.12-0.16 part of proline, 0.2-0.4 part of glucose, 0.4-0.5 part of potassium dihydrogen phosphate and 0.6-1.0 part of calcium chloride.
Preferably, the material comprises the following raw materials in parts by weight: 5 parts of bacillus subtilis, 11 parts of purple sulfur bacteria, 15 parts of rhizobium, 1.5 parts of trichoderma harzianum, 0.15 part of proline, 0.3 part of glucose, 0.45 part of potassium dihydrogen phosphate and 0.8 part of calcium chloride.
The bacillus subtilis has strong heat resistance and stress resistance, is nontoxic and harmless to people and livestock, does not pollute the environment, has direct or indirect inhibition effect on some pathogenic bacteria, can decompose harmful substances, garbage particles and the like, can promote plant root systems and plant growth by secreting hormone substances, various enzymes and antibacterial substances, enhances the disease resistance, drought resistance and salt and alkali resistance of plants, and improves the growth and development of plants. According to the invention, the research shows that the addition amount of the bacillus subtilis directly influences the improvement effect on the soil, and the salt and alkali degree of the soil cannot be well improved even if the addition amount of the bacillus subtilis is too high or too low.
The purple sulfur bacteria are photosynthetic bacteria, can oxidize hydrogen sulfide to generate sulfuric acid under anaerobic conditions, can improve the solubility of various mineral substances in soil, has unique disease resistance and growth promotion capability, and can improve crop yield. Rhizobium has nitrogen fixation effect. Trichoderma harzianum can improve the microbial flora structure of soil by regulating soil nutrients, and stimulate and induce crops to generate stress resistance.
In the research of the invention, the effective viable count of each strain in the mixed microbial agent is possibly lower than that of each strain when the mixed microbial agent singly grows when different types of microorganisms are mixed. And because of colony effect among different microorganisms, when different bacteria form a mixed microbial inoculum, the beneficial effect of the original strain can be changed, and the improvement effect on soil is affected. When a plurality of strains are mixed, the metabolic processes of the strains are influenced simultaneously due to different adding proportions of the strains, so that the products in the microbial inoculum are different in types and concentrations, and the soil improvement effect is obviously different.
The microbial cultivated land soil restoration agent provided by the invention has the advantages that all strains are mixed according to a proportion, and after the microbial cultivated land soil restoration agent is applied to soil, proline, glucose, monopotassium phosphate and calcium chloride are taken as nutrients to proliferate and grow, so that the microbial cultivated land soil restoration agent can quickly become dominant bacterial groups in the soil, can exert excellent effect of improving the soil, can effectively reduce the saline-alkali degree of the soil, increase the air permeability and the water permeability of the soil, improve the hardening condition of the soil and improve the soil fertility.
In the invention, the bacillus subtilis, the purple sulfur bacteria, the rhizobium and the trichoderma harzianum are respectively liquid-cultured bacillus subtilis liquid, purple sulfur bacteria liquid, rhizobium liquid and trichoderma harzianum liquid.
The liquid culture method of each strain is not particularly limited, and the conventional liquid culture method of each strain in the field is adopted.
Another object of the present invention is to provide a method for restoring cultivated land soil by microorganisms, comprising the steps of:
(1) Weighing the microbial farmland soil restoration agent, and mixing the microbial farmland soil restoration agent with water to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the depth of the holes is 5-10cm, the diameter of the holes is 1-2cm, the interval between the holes is 30-50cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, soaking the field for 18-25 days, and draining water;
(3) 10 days before crop sowing, rotary tillage soil preparation is carried out on the field, the liquid soil restoration agent is applied to soil along with irrigation water, and water is drained after 3-8 hours of field soaking.
In the invention, the liquid soil restoration agent is prepared by mixing microbial cultivated land soil restoration agent and water according to a mass ratio of 1:05-0.8.
In the invention, the soaking field in the step (2) and the step (3) is that the water depth is 1-5cm beyond the soil layer.
In the invention, the application amount of the liquid soil restoration agent in the step (2) is 50-80 kg/mu; the application amount of the liquid soil restoration agent in the step (3) is 100-130 kg/mu.
In the invention, in the step (2), the surface of the field block is covered with a plastic film or plant straw after the field is soaked and drained.
In the invention, the thickness of the plastic film is more than or equal to 0.02mm; the plant straw coverage thickness is 5-10cm.
According to the invention, salt in the soil can be effectively leached through soaking the field, and the pH of the soil is reduced. After the water is drained from the soaked fields, the surfaces of the field blocks are covered with plastic films or plant straws, so that the salt inhibition effect is good.
According to the invention, the microbial soil conditioner is applied to the field along with irrigation water to soak the field, and the field is perforated, so that the soil conditioner penetrates into the soil and forms dominant bacteria in the soil rapidly to exert the beneficial effects.
According to the invention, salt is washed on the soil through the field soaking treatment, and the surface of the field is covered after the field soaking is finished, so that the field salt is prevented from being reversely salted, and the salt content of the soil can be effectively reduced.
Unless specifically stated otherwise, reagents, consumables and the like according to the present invention are commercially available, and are usually carried out under conventional conditions or under conditions recommended by the company, unless specific conditions of use are specified.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The embodiment provides a microbial cultivated land soil restoration method
The microbial cultivated land soil restoration agent comprises the following raw materials in parts by weight: 5 parts of bacillus subtilis, 11 parts of purple sulfur bacteria, 15 parts of rhizobium, 1.5 parts of trichoderma harzianum, 0.15 part of proline, 0.3 part of glucose, 0.45 part of potassium dihydrogen phosphate and 0.8 part of calcium chloride.
(1) Weighing the microbial farmland soil restoration agent, and mixing the microbial farmland soil restoration agent with water according to a mass ratio of 1:0.5 to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the depth of each hole is 6cm, the diameter is 2cm, the interval between the holes is 35cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, and draining water after the water depth is 5cm beyond the soil layer and the field is soaked for 20 days; the field blocks cover 5cm of wheat straw after water drainage; the application amount of the liquid soil restoration agent is 60 kg/mu;
(3) 10 days before crop sowing, carrying out rotary tillage and soil preparation on the field, directly carrying out rotary tillage and returning of the wheat straw to the field during rotary tillage, applying the liquid soil restoration agent into soil along with irrigation water, soaking the field for 6 hours after the water depth is 3cm, and draining; the application amount of the liquid soil restoration agent is 120 kg/mu.
Example 2
The embodiment provides a microbial cultivated land soil restoration method
The microbial cultivated land soil restoration agent comprises the following raw materials in parts by weight: 6 parts of bacillus subtilis, 10 parts of purple sulfur bacteria, 12 parts of rhizobium, 2 parts of trichoderma harzianum, 0.12 part of proline, 0.2 part of glucose, 0.5 part of monopotassium phosphate and 0.9 part of calcium chloride.
(1) Weighing the microbial farmland soil restoration agent, and mixing the microbial farmland soil restoration agent with water according to a mass ratio of 1:0.8 to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the hole depth is 8cm, the diameter is 1.5cm, the interval between the holes is 40cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, the water depth is 3cm beyond the soil layer, and draining after soaking the field for 23 days; covering the field with plastic film with thickness more than or equal to 0.02mm after draining; the application amount of the liquid soil restoration agent is 70 kg/mu;
(3) 10 days before crop sowing, rotary tillage soil preparation is carried out on the field, plastic films are removed before rotary tillage, the liquid soil restoration agent is applied into soil along with irrigation water, the water depth is 2cm beyond the soil layer, and drainage is carried out after field soaking for 5 hours; the application amount of the liquid soil restoration agent is 100 kg/mu.
Example 3
The embodiment provides a microbial cultivated land soil restoration method
The microbial cultivated land soil restoration agent comprises the following raw materials in parts by weight: 7 parts of bacillus subtilis, 13 parts of purple sulfur bacteria, 15 parts of rhizobia, 1 part of trichoderma harzianum, 0.08 part of proline, 0.4 part of glucose, 0.3 part of monopotassium phosphate and 0.5 part of calcium chloride.
(1) Weighing the microbial farmland soil restoration agent, and mixing the microbial farmland soil restoration agent with water according to a mass ratio of 1:0.6 to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the depth of the holes is 6cm, the diameter is 1.5cm, the interval between the holes is 50cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, and draining water after the water depth is 4cm beyond the soil layer and the field is soaked for 19 days; the field blocks cover 8cm of wheat straw after water drainage; the application amount of the liquid soil restoration agent is 55 kg/mu;
(3) 10 days before crop sowing, carrying out rotary tillage and soil preparation on the field, directly carrying out rotary tillage and returning of the wheat straw to the field during rotary tillage, applying the liquid soil restoration agent into soil along with irrigation water, soaking the field for 7 hours after the water depth is 2cm, and draining; the application amount of the liquid soil restoration agent is 115 kg/mu.
Comparative example 1
The comparative example differs from example 1 in that the microbial cultivated land soil restoration agent comprises the following raw materials in parts by weight: 2 parts of bacillus subtilis, 11 parts of purple sulfur bacteria, 15 parts of rhizobium, 4 parts of trichoderma harzianum, 0.08 part of proline, 0.4 part of glucose, 0.3 part of monopotassium phosphate and 0.5 part of calcium chloride.
Comparative example 2
The comparative example differs from example 1 in that the microbial cultivated land soil restoration agent comprises the following raw materials in parts by weight: 10 parts of bacillus subtilis, 20 parts of purple sulfur bacteria, 5 parts of rhizobium, 1.5 parts of trichoderma harzianum, 0.08 part of proline, 0.4 part of glucose, 0.3 part of potassium dihydrogen phosphate and 0.5 part of calcium chloride.
Comparative example 3
The comparative example differs from example 1 in that no Bacillus subtilis was added to the microbial farmland soil restoration agent.
Comparative example 4
The comparative example is different from example 1 in that rhizobium and trichoderma harzianum are not added to the microbial farmland soil restoration agent.
Comparative example 5
The comparative example differs from example 1 in that proline and glucose are not added to the microbial farmland soil restoration agent.
Comparative example 6
The difference between this comparative example and example 1 is that the field is soaked directly after the harvest of the previous crop, and no holes are punched in the field.
Comparative example 7
This comparative example differs from example 1 in that no field soaking treatment was performed after harvest of the previous crop.
Comparative example 8
The comparative example is different from example 1 in that the field was prepared by rotary tillage only 10 days before sowing, and no field soaking treatment was performed.
Example 4
This example compares the effect of different methods to improve the soil of a cultivated land.
The treatment of the field was carried out in a saline-alkali soil test field by the methods described in examples 1 to 3 and comparative examples 1 to 8, and the untreated field was used as a control and denoted as CK. After the field treatment is finished, preparing land by adopting a local conventional method, planting peanuts, wherein the peanut variety is Luhua No. 9, preparing land at the same time by all the treatments, sowing at the same time, and adopting local conventional field management after sowing.
After harvesting peanuts, the yields of the peanuts treated differently were counted and the salt content, pH value, organic matter content and soil particle size percentage at 0-20cm of the soil were measured, and the results are shown in Table 1.
TABLE 1 soil improvement effect of different treatments of cultivated lands
As can be seen from the results in Table 1, the method for restoring farmland soil by microorganisms provided by the invention can effectively reduce the salt content of the soil at 0-20cm, reduce the pH value of the soil, improve the organic matter content and improve the yield of crops. Meanwhile, according to the soil particle size distribution at the position of 0-20cm of soil, the method provided by the invention improves the content of small aggregates with the particle size of 0.25-2mm and large aggregates with the particle size of more than 2mm, reduces the proportion of soil with the particle size of less than 0.25mm, and shows that the method can improve the soil aggregation structure, reduce the hardening property of the soil and improve the physical properties of the soil.
According to the test results of the comparative examples, when the composition of the strains in the microbial soil conditioner provided by the invention is changed or the adding proportion of each strain is changed, the synergistic effect among microorganisms is affected, dominant bacteria cannot be quickly formed after the microbial soil conditioner is applied to the soil, metabolites are changed, and the soil improvement effect is obviously reduced.
Therefore, the invention can effectively reduce the saline-alkali degree of the soil, increase the air permeability and the water permeability of the soil, improve the hardening condition of the soil, and improve the fertility of the cultivated land soil, thereby improving the crop yield. Is helpful for improving the saline-alkali soil conditions and promoting the benign development of agriculture.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. The microbial farmland soil restoration agent is characterized by comprising the following raw materials in parts by weight: 4-8 parts of bacillus subtilis, 8-15 parts of purple sulfur bacteria, 10-17 parts of rhizobium, 1-3 parts of trichoderma harzianum, 0.05-0.2 part of proline, 0.1-0.5 part of glucose, 0.4-1.2 parts of potassium dihydrogen phosphate and 0.3-0.6 part of calcium chloride;
The bacillus subtilis, the purple sulfur bacteria, the rhizobium and the trichoderma harzianum are respectively liquid cultured bacillus subtilis liquid, purple sulfur bacteria liquid, rhizobium liquid and trichoderma harzianum liquid.
2. The microbial cultivated land soil restoration agent according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 5-6 parts of bacillus subtilis, 10-12 parts of purple sulfur bacteria, 13-15 parts of rhizobium, 1.5-2 parts of trichoderma harzianum, 0.12-0.16 part of proline, 0.2-0.4 part of glucose, 0.6-1.0 part of potassium dihydrogen phosphate and 0.4-0.5 part of calcium chloride.
3. The microbial cultivated land soil restoration agent according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 5 parts of bacillus subtilis, 11 parts of purple sulfur bacteria, 15 parts of rhizobium, 1.5 parts of trichoderma harzianum, 0.15 part of proline, 0.3 part of glucose, 0.8 part of potassium dihydrogen phosphate and 0.45 part of calcium chloride.
4. A method for repairing cultivated land soil by microorganisms, comprising the following steps:
(1) Weighing the microbial farmland soil restoration agent according to any one of claims 1-3, and mixing the microbial farmland soil restoration agent with water to obtain a liquid soil restoration agent;
(2) After harvesting the previous crop, punching holes in the field, wherein the depth of the holes is 5-10cm, the diameter of the holes is 1-2cm, the interval between the holes is 30-50cm, applying the liquid soil restoration agent into the field along with irrigation water after punching, soaking the field for 18-25 days, and draining water;
(3) 10 days before crop sowing, rotary tillage soil preparation is carried out on the field, the liquid soil restoration agent is applied to soil along with irrigation water, and water is drained after 3-8 hours of field soaking.
5. The method for restoring farmland soil according to claim 4, wherein the liquid soil restoration agent is a mixture of the microbial farmland soil restoration agent and water in a mass ratio of 1:0.5-0.8.
6. The method for restoring farmland soil according to claim 4, wherein said steeping field in step (2) and step (3) is maintained at a water depth of 1-5cm across the soil layer.
7. The method for restoring farmland soil according to claim 4, wherein the liquid soil restoration agent application amount in step (2) is 50-80 kg/mu; the application amount of the liquid soil restoration agent in the step (3) is 100-130 kg/mu.
8. The method for restoring farmland soil according to claim 4, wherein the surface of the field pieces after the soaking field drainage in step (2) is covered with plastic film or plant stalks.
9. The method for restoring farmland soil by microorganisms according to claim 8, wherein the thickness of the plastic film is not less than 0.02mm; the plant straw coverage thickness is 5-10cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310123823.2A CN116004246B (en) | 2023-02-15 | 2023-02-15 | Microbial farmland soil restoration agent and microbial farmland soil restoration method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310123823.2A CN116004246B (en) | 2023-02-15 | 2023-02-15 | Microbial farmland soil restoration agent and microbial farmland soil restoration method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116004246A CN116004246A (en) | 2023-04-25 |
| CN116004246B true CN116004246B (en) | 2024-05-24 |
Family
ID=86033669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310123823.2A Active CN116004246B (en) | 2023-02-15 | 2023-02-15 | Microbial farmland soil restoration agent and microbial farmland soil restoration method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116004246B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117343884B (en) * | 2023-12-06 | 2024-02-23 | 山东君德生态科技有限公司 | Preparation method of microbial agent for improving saline-alkali soil |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609979A (en) * | 2015-02-04 | 2015-05-13 | 北京东方力原生物科技有限公司 | Microbial fertilizer for improving saline alkali soil and preparation method thereof |
| CN106588424A (en) * | 2016-08-31 | 2017-04-26 | 山东胜伟园林科技有限公司 | Controlled release fertilizer special for saline alkali soil improvement |
| CN106888603A (en) * | 2015-12-20 | 2017-06-27 | 衡阳智源农业科技有限公司 | A kind of saline-alkali soil improvement method |
| CN108076718A (en) * | 2017-12-25 | 2018-05-29 | 山东胜景旅游发展有限公司 | A kind of coastal saline-alkali soil improving technology |
| CN108997035A (en) * | 2018-09-30 | 2018-12-14 | 张元生 | The method for improveing the microbial-bacterial fertilizer in salt-soda soil and preparation method thereof and improvement soil |
| CN109306328A (en) * | 2017-07-27 | 2019-02-05 | 天津市天大天福生物技术有限公司 | Saline-alkali tolerant bacterial manure microorganism be separately cultured and serial bacterial fertilizer products production technology |
| CN109912352A (en) * | 2019-04-17 | 2019-06-21 | 河西学院 | A kind of low temperature resistant liquid fertilizer promoting crop photosynthesis |
| CN110241041A (en) * | 2019-05-31 | 2019-09-17 | 南京工业大学 | A kind of complex microorganism preparations, preparation method and application |
| CN111602484A (en) * | 2020-05-27 | 2020-09-01 | 吉林省林业科学研究院 | Method for improving saline-alkali soil by utilizing vegetation |
| CN112481167A (en) * | 2020-12-03 | 2021-03-12 | 黑龙江沪生农业发展有限公司 | Compound microbial agent for improving soil fertility and preparation method thereof |
| CN115181706A (en) * | 2022-08-18 | 2022-10-14 | 山东省农业技术推广中心(山东省农业农村发展研究中心) | Microbial agent for livestock and poultry manure compost and preparation method thereof |
| CN115386380A (en) * | 2021-05-20 | 2022-11-25 | 中达翰星环境修复有限公司 | Saline-alkali soil improvement ecological restoration agent and preparation method thereof |
-
2023
- 2023-02-15 CN CN202310123823.2A patent/CN116004246B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104609979A (en) * | 2015-02-04 | 2015-05-13 | 北京东方力原生物科技有限公司 | Microbial fertilizer for improving saline alkali soil and preparation method thereof |
| CN106888603A (en) * | 2015-12-20 | 2017-06-27 | 衡阳智源农业科技有限公司 | A kind of saline-alkali soil improvement method |
| CN106588424A (en) * | 2016-08-31 | 2017-04-26 | 山东胜伟园林科技有限公司 | Controlled release fertilizer special for saline alkali soil improvement |
| CN109306328A (en) * | 2017-07-27 | 2019-02-05 | 天津市天大天福生物技术有限公司 | Saline-alkali tolerant bacterial manure microorganism be separately cultured and serial bacterial fertilizer products production technology |
| CN108076718A (en) * | 2017-12-25 | 2018-05-29 | 山东胜景旅游发展有限公司 | A kind of coastal saline-alkali soil improving technology |
| CN108997035A (en) * | 2018-09-30 | 2018-12-14 | 张元生 | The method for improveing the microbial-bacterial fertilizer in salt-soda soil and preparation method thereof and improvement soil |
| CN109912352A (en) * | 2019-04-17 | 2019-06-21 | 河西学院 | A kind of low temperature resistant liquid fertilizer promoting crop photosynthesis |
| CN110241041A (en) * | 2019-05-31 | 2019-09-17 | 南京工业大学 | A kind of complex microorganism preparations, preparation method and application |
| CN111602484A (en) * | 2020-05-27 | 2020-09-01 | 吉林省林业科学研究院 | Method for improving saline-alkali soil by utilizing vegetation |
| CN112481167A (en) * | 2020-12-03 | 2021-03-12 | 黑龙江沪生农业发展有限公司 | Compound microbial agent for improving soil fertility and preparation method thereof |
| CN115386380A (en) * | 2021-05-20 | 2022-11-25 | 中达翰星环境修复有限公司 | Saline-alkali soil improvement ecological restoration agent and preparation method thereof |
| CN115181706A (en) * | 2022-08-18 | 2022-10-14 | 山东省农业技术推广中心(山东省农业农村发展研究中心) | Microbial agent for livestock and poultry manure compost and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 买永彬，傅克文主编.《灌溉水质标准研究》.农业出版社,1985,第4-5页. * |
| 周文嘉，金志南编.《土壤》.山西人民出版社,1983,第833-834页. * |
| 薛循革主编.《浦东新区耕地地力与质量研究》.上海科学普及出版社,2006,第61-62页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116004246A (en) | 2023-04-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109400372B (en) | Biochar soil improvement organic fertilizer and preparation method thereof | |
| CN108410470B (en) | Microbial soil conditioner for saline-alkali soil and preparation method thereof | |
| CN104774094A (en) | Preparation method of compound microbial seaweed fertilizer | |
| CN111440616A (en) | Saline-alkali soil conditioner and preparation method thereof | |
| CN102349551A (en) | Plant culturing medium regulator and preparation method thereof | |
| CN113214000A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN110004091A (en) | A kind of complex microbial inoculum, soil-repairing agent and preparation method and application | |
| CN116004246B (en) | Microbial farmland soil restoration agent and microbial farmland soil restoration method | |
| CN107473861A (en) | A kind of special complex fertilizer for alleviating citrus yellow and preparation method thereof | |
| CN106068746A (en) | A kind of salt-soda soil biological modification method | |
| CN114231452B (en) | Soil improvement method for crop planting | |
| CN115925486A (en) | Seaweed compound microbial fertilizer for preventing and treating soil moss and preparation method thereof | |
| CN113620730B (en) | Organic fertilizer formula for roses | |
| CN107353127A (en) | A kind of microorganism Water soluble fertilizer for preventing and treating corn fungal disease | |
| CN101288359B (en) | Method for stopping clover seeds dormancy in hard seeds | |
| US20240002308A1 (en) | Soil-structure improving bio-organic fertilizer and preparation method thereof | |
| CN113897316B (en) | Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application | |
| CN113583917A (en) | Compound microbial agent for ecological restoration and preparation method thereof | |
| CN113149727A (en) | Preparation method of biological agent organic fertilizer | |
| CN115322056A (en) | Application of water-retaining agent and application of composite bio-organic fertilizer with water-retaining function | |
| CN112369303A (en) | Preparation method of organic soilless culture substrate for cucumbers | |
| CN111592402A (en) | Method for improving fermentation efficiency of pig manure | |
| CN110679431A (en) | Peach seed emergence substrate and seedling raising method | |
| NL2032380B1 (en) | Soil conditioner and preparation method thereof | |
| CN110684540B (en) | Complex microbial preparation containing indigenous microorganisms and method for improving farmland |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |