CN113583917A - Compound microbial agent for ecological restoration and preparation method thereof - Google Patents
Compound microbial agent for ecological restoration and preparation method thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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Abstract
The invention belongs to the technical field of ecological restoration, and relates to a compound microbial agent for ecological restoration and a preparation method thereof, wherein the compound microbial agent for ecological restoration comprises, by weight, 0.1-0.3 part of thermal amylase streptomyces, 0.5-1 part of bacillus megaterium, 0.1-0.5 part of radiorhizobium, 1-3 parts of paecilomyces lilacinus and 0.5-2 parts of streptomyces griseus. The composite microbial agent for ecological restoration provided by the invention has a good soil restoration effect.
Description
Technical Field
The invention belongs to the technical field of ecological restoration, and particularly relates to a compound microbial agent for ecological restoration and a preparation method thereof.
Background
In many areas of China, a series of problems such as nutrient loss, deterioration of cultivated land soil, shallow plough layer, poor ploughing property, soil impoverishment, soil hardening, soil salinization and the like are caused by predatory cultivation for decades, and the ecological environment balance is seriously damaged.
Among them, the saline-alkali soil currently accounts for about one tenth of the world total amount in China, and about 20% of agricultural irrigation land is affected by salinization, wherein arid and semiarid saline-alkali soil accounts for 54%, which is the biggest environmental problem in the arid land agriculture. Therefore, the method actively researches and explores the treatment measures of the salinization of the soil, improves the effective utilization of soil resources, improves the ecological environment and promotes the sustainable development of agriculture, and has been generally regarded by the society. The existing treatment measures of the saline-alkali soil are mainly to utilize chemical substances to carry out saline-alkali restoration on the soil, and chemical reagents are used for reacting with the chemical substances in the soil to reduce saline-alkali components in the soil; the method has the advantages of quick effect and good short-term effect; but the cost is high, chemical agents are not easy to remove after entering the soil, and the physical chemical and biological properties of the soil are changed after long-term use, and the adverse physical chemical and biological properties can influence the normal growth of crops.
Because the soil contains a large amount of nutrient elements such as C, H, O, N, P, S and the like required by microorganisms, saline-alkali components in saline-alkali soil can be decomposed, transformed, absorbed and utilized through the growth and reproduction effects of the microorganisms, and the physical, chemical and biological properties of the saline-alkali soil are changed, so that the saline-alkali soil is beneficial to normal growth. Therefore, the specific microorganism is applied to environmental protection and soil remediation, and the environmental pollution damage factors can be continuously utilized, degraded and converted, so that the polluted and damaged soil can be safely and long-term remedied.
Disclosure of Invention
The invention aims to provide a compound microbial agent for ecological restoration, which aims to solve the problem that the normal growth of crops is influenced when chemical substances are utilized to carry out saline-alkali restoration on soil at present.
The second purpose of the invention is to provide a preparation method of the compound microbial agent for ecological restoration.
The purpose of the invention is realized by the following technical scheme:
the invention provides a compound microbial agent for ecological restoration, which comprises, by weight, 0.1-0.3 part of thermal amylase streptomyces, 0.5-1 part of bacillus megaterium, 0.1-0.5 part of radiorhizobium, 1-3 parts of paecilomyces lilacinus and 0.5-2 parts of streptomyces griseus.
In a preferred embodiment, the complex microbial inoculant for ecological restoration comprises:
a thermal amylase streptomyces at a concentration of 10 hundred million cfu/g or more, for example, the thermal amylase streptomyces can be at a concentration of 10 hundred million cfu/g, 11 hundred million cfu/g, 12 hundred million cfu/g, 13 hundred million cfu/g, 14 hundred million cfu/g, 15 hundred million cfu/g, 16 hundred million cfu/g, 17 hundred million cfu/g, 18 hundred million cfu/g, 19 hundred million cfu/g, 20 hundred million cfu/g, 25 hundred million cfu/g, 30 hundred million cfu/g, 35 hundred million cfu/g, 40 hundred million cfu/g, 45 hundred million cfu/g, or 50 hundred million cfu/g;
bacillus megaterium at a concentration of 0.3-1 hundred million cfu/g, e.g., the Bacillus megaterium can be at a concentration of 0.3 hundred million cfu/g, 0.4 hundred million cfu/g, 0.5 hundred million cfu/g, 0.6 hundred million cfu/g, 0.7 hundred million cfu/g, 0.8 hundred million cfu/g, 0.9 hundred million cfu/g, or 1 hundred million cfu/g;
radiorhizobium at a concentration of 1-2 hundred million cfu/g, for example, the radiorhizobium may be at a concentration of 1cfu/g, 1.1cfu/g, 1.2cfu/g, 1.3cfu/g, 1.4cfu/g, 1.5cfu/g, 1.6cfu/g, 1.7cfu/g, 1.8cfu/g, 1.9cfu/g, or 2 cfu/g;
paecilomyces lilacinus at a concentration of 0.6-1 hundred million cfu/g, for example, the paecilomyces lilacinus may be at a concentration of 0.6 hundred million cfu/g, 0.7 hundred million cfu/g, 0.8 hundred million cfu/g, 0.9 hundred million cfu/g, or 1 hundred million cfu/g;
streptomyces griseus at a concentration of 0.6-1 hundred million cfu/g, for example, the Streptomyces griseus can be at a concentration of 0.6 hundred million cfu/g, 0.7 hundred million cfu/g, 0.8 hundred million cfu/g, 0.9 hundred million cfu/g, or 1 hundred million cfu/g.
In a preferred embodiment, the complex microbial agent for ecological restoration comprises 0.1-0.3 parts of thermal amylase streptomyces, 0.5-1 parts of bacillus megaterium, 0.1-0.5 parts of radiorhizobium, 1-3 parts of paecilomyces lilacinus and 0.5-2 parts of streptomyces griseus according to parts by weight.
In a preferred embodiment, the complex microbial agent for ecological restoration comprises 0.2 parts of thermal amylase streptomyces, 0.6 parts of bacillus megaterium, 0.3 parts of radiorhizobium, 2 parts of paecilomyces lilacinus and 1 part of streptomyces griseus according to parts by weight.
In a preferred embodiment, the compound microbial agent for ecological restoration further comprises 10-15 parts of tartaric acid, 5-8 parts of zeolite powder, 10-20 parts of magnesium sulfate, 13-15 parts of cellulase and 25-30 parts of wormcast.
In a preferred embodiment, the compound microbial agent for ecological restoration further comprises 11 parts by weight of tartaric acid, 6 parts by weight of zeolite powder, 15 parts by weight of magnesium sulfate, 14 parts by weight of cellulase and 27 parts by weight of wormcast.
The invention provides a preparation method of a compound microbial agent for ecological restoration, which comprises the following steps:
s1, firstly, mixing tartaric acid, zeolite powder and magnesium sulfate, then adding cellulase and wormcast, mixing again to obtain a mixed material, fermenting the mixed material for 5-8 days, sterilizing at 80-100 ℃ for 0.5-1h, and cooling to room temperature to obtain an adsorption carrier;
s2, sequentially adsorbing the thermal amylase streptomyces, the bacillus megatherium, the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus on the adsorption carrier obtained in the step S1, stirring, air-drying and crushing to obtain the compound microbial agent for ecological restoration.
Compared with the prior art, the invention has the beneficial effects that:
the specific microorganisms are synergistic to continuously utilize, degrade and convert environmental pollution damage factors, so that the polluted and damaged soil is safely and long-acting repaired.
The tartaric acid, the zeolite powder, the magnesium sulfate, the cellulase and the wormcast used in the composite microbial agent for ecological restoration provided by the invention are prepared into the adsorption carrier, so that the microorganisms can be adsorbed to the maximum extent, the utilization rate of the adsorption carrier and the microorganisms is improved, and the polluted and damaged soil is better restored.
The specific microorganisms used by the invention are matched with the specific adsorption carriers used by the invention, so that the polluted and damaged soil can be maximally repaired, particularly the saline-alkali soil can be maximally repaired, and the growth of plants can be better promoted.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Example 1
The embodiment provides a compound microbial agent for ecological restoration, which comprises, by weight, 0.1 part of thermal amylase streptomyces, 0.5 part of bacillus megatherium, 0.1 part of radiorhizobium, 1 part of paecilomyces lilacinus, 0.5 part of streptomyces griseus, 10 parts of humic acid, 5 parts of zeolite powder, 10 parts of magnesium sulfate, 13 parts of cellulase and 25 parts of wormcast; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Example 2
The embodiment provides a compound microbial agent for ecological restoration, which comprises, by weight, 0.3 parts of thermal amylase streptomyces, 1 part of bacillus megatherium, 0.5 parts of radiorhizobium, 3 parts of paecilomyces lilacinus, 2 parts of streptomyces griseus, 15 parts of humic acid, 8 parts of zeolite powder, 20 parts of magnesium sulfate, 15 parts of cellulase and 30 parts of wormcast; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Example 3
The embodiment provides a compound microbial agent for ecological restoration, which comprises, by weight, 0.2 parts of thermal amylase streptomyces, 0.6 parts of bacillus megatherium, 0.3 parts of radiorhizobium, 2 parts of paecilomyces lilacinus, 1 part of streptomyces griseus, 11 parts of tartaric acid, 6 parts of zeolite powder, 15 parts of magnesium sulfate, 14 parts of cellulase and 27 parts of wormcast; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Example 4
The embodiment provides a preparation method of a compound microbial agent for ecological restoration, which comprises the following steps:
s1, firstly, mixing tartaric acid, zeolite powder and magnesium sulfate, then adding cellulase and wormcast, mixing again to obtain a mixed material, fermenting the mixed material for 5-8 days, sterilizing at 80-100 ℃ for 0.5-1h, and cooling to room temperature to obtain an adsorption carrier;
s2, sequentially adsorbing the thermal amylase streptomyces, the bacillus megatherium, the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus on the adsorption carrier obtained in the step S1, stirring, air-drying and crushing to obtain the compound microbial agent for ecological restoration.
Comparative example 1
The comparative example provides a compound microbial agent for ecological restoration, which comprises 0.6 part of bacillus megaterium, 0.3 part of radiorhizobium, 2 parts of paecilomyces lilacinus, 1 part of streptomyces griseus, 11 parts of tartaric acid, 6 parts of zeolite powder, 15 parts of magnesium sulfate, 14 parts of cellulase and 27 parts of wormcast according to parts by weight; wherein the content of the first and second substances,
the concentration of the bacillus megaterium is 0.5 hundred million cfu/g, the concentration of the radiorhizobium is 1.2 hundred million cfu/g, the concentration of the paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of the streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 2
The comparative example provides a compound microbial agent for ecological restoration, which comprises 0.3 part of radiorhizobium, 2 parts of paecilomyces lilacinus, 1 part of streptomyces griseus, 11 parts of tartaric acid, 6 parts of zeolite powder, 15 parts of magnesium sulfate, 14 parts of cellulase and 27 parts of wormcast according to parts by weight; wherein the content of the first and second substances,
the concentration of the radiorhizobium is 1.2 hundred million cfu/g, the concentration of the paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of the streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 3
The comparative example provides a compound microbial agent for ecological restoration, which comprises 2 parts by weight of paecilomyces lilacinus, 1 part by weight of streptomyces griseus, 11 parts by weight of tartaric acid, 6 parts by weight of zeolite powder, 15 parts by weight of magnesium sulfate, 14 parts by weight of cellulase and 27 parts by weight of wormcast; wherein the content of the first and second substances,
the concentration of the paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of the streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 4
The comparative example provides a compound microbial agent for ecological restoration, which comprises 1 part of streptomyces griseus, 11 parts of tartaric acid, 6 parts of zeolite powder, 15 parts of magnesium sulfate, 14 parts of cellulase and 27 parts of wormcast in parts by weight; wherein the content of the first and second substances,
the concentration of Streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 5
The comparative example provides a compound microbial agent for ecological restoration, which comprises 0.2 part of thermal amylase streptomyces, 0.6 part of bacillus megatherium, 0.3 part of radiorhizobium, 2 parts of paecilomyces lilacinus, 1 part of streptomyces griseus, 11 parts of humic acid, 6 parts of zeolite powder, 15 parts of magnesium sulfate, 14 parts of cellulase and 27 parts of wormcast according to parts by weight; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 6
The comparative example provides a compound microbial agent for ecological restoration, which comprises 0.05 part of thermal amylase streptomyces, 0.3 part of bacillus megatherium, 0.05 part of radiorhizobium, 0.5 part of paecilomyces lilacinus, 0.3 part of streptomyces griseus, 8 parts of tartaric acid, 3 parts of zeolite powder, 8 parts of magnesium sulfate, 12 parts of cellulase and 22 parts of wormcast according to parts by weight; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Comparative example 7
The comparative example provides a compound microbial agent for ecological restoration, which comprises 0.5 part of thermal amylase streptomyces, 1.2 parts of bacillus megatherium, 0.6 part of radiorhizobium, 5 parts of paecilomyces lilacinus, 3 parts of streptomyces griseus, 16 parts of tartaric acid, 10 parts of zeolite powder, 22 parts of magnesium sulfate, 16 parts of cellulase and 32 parts of wormcast according to parts by weight; wherein the content of the first and second substances,
the concentration of thermal amylase streptomycete is 12 hundred million cfu/g, the concentration of bacillus megatherium is 0.5 hundred million cfu/g, the concentration of radiorhizobium is 1.2 hundred million cfu/g, the concentration of paecilomyces lilacinus is 0.8 hundred million cfu/g, and the concentration of streptomyces griseus is 0.8 hundred million cfu/g.
Effect verification
Subject: examples 1-3 and comparative examples 1-7 the complex microbial inoculant for ecological restoration prepared by the method
The experimental method comprises the following steps: the compound microbial agent for ecological restoration prepared in the examples 1-3 and the comparative examples 1-7 is added into the moderate saline-alkali soil of a certain place, corns are planted in the saline-alkali soil added with the compound microbial agent for ecological restoration prepared in the examples 1-3 and the comparative examples 1-7, and the changes of the physicochemical and biological properties of the soil and the growth condition of the corns are observed.
The addition pattern was repeated 3 times per group for a total of 30 pots and was randomly arranged. Selecting corn seeds with uniform size and no plant diseases and insect pests, disinfecting the corn seeds for 30min by using 10% hydrogen peroxide, washing the corn seeds for 2-3 times by using tap water, and finally cleaning the corn seeds by using distilled water for accelerating germination. The sprouted corns are sown in pots in a non-contact mode according to 2 grains per pot, seedlings are cut uniformly in the two-leaf period of the seedlings, and the growth vigor of the seedlings to be treated is basically consistent. Regularly observing every day and uniformly watering in time. And collecting samples after the seedlings grow for 45 days, and respectively determining the root length, the root surface department and the root volume of each pot of corn seedlings by adopting destructive sampling.
The experimental results are shown in tables 1, 2 and 3 below.
TABLE 1 changes in physicochemical Properties of the soil
Further, the air conditioner is provided with a fan,
comparative example 1.1 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium;
comparative example 1.2 is provided, which is distinguished from example 3 by the following: no radiorhizobium was added;
comparative example 1.3 is provided, which is distinguished from example 3 by the following: no paecilomyces lilacinus was added;
comparative example 1.4 is provided, which is distinguished from example 3 by the following: streptomyces griseus was not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the physicochemical properties of the soil of the complex microbial agents for ecological restoration prepared in comparative example 1.1, comparative example 1.2, comparative example 1.3, and comparative example 1.4 were observed, and the results were similar to those in comparative example 1 described above.
Further, the air conditioner is provided with a fan,
comparative example 2.1 is provided, which is distinguished from example 3 by the following: no amylase streptomyces and radiorhizobium were added;
comparative example 2.2 is provided, which is distinguished from example 3 by the following: no thermal amylase chain mold and paecilomyces lilacinus added;
comparative example 2.3 is provided, which comparative example 2.3 differs from example 3 in that: no Thermoamylase Streptomyces and Streptomyces griseus were added;
comparative example 2.4 is provided, which comparative example 2.4 differs from example 3 in that: no Bacillus megaterium and radiorhizobium were added;
comparative example 2.5 is provided, which is distinguished from example 3 by the following: the bacillus megaterium and the paecilomyces lilacinus are not added;
comparative example 2.6 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium and Streptomyces griseus;
comparative example 2.7 is provided, which is distinguished from example 3 by the following: no radiorhizobium or paecilomyces lilacinus was added;
comparative example 2.8 is provided, which is distinguished from example 3 by the following: no radiorhizobium or streptomyces griseus added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the physicochemical properties of the soil of the complex microbial agents for ecological restoration prepared in comparative example 2.1, comparative example 2.2, comparative example 2.3, comparative example 2.4, comparative example 2.5, comparative example 2.6, comparative example 2.7, and comparative example 2.8 were observed, and the results were similar to those in comparative example 2 described above.
Further, the air conditioner is provided with a fan,
comparative example 3.1 is provided, which comparative example 3.1 differs from example 3 in that: no amylase streptomyces and radiorhizobium were added;
comparative example 3.2 is provided, which comparative example 3.2 differs from example 3 in that: does not add thermal amylase chain mould, bacillus megaterium and paecilomyces lilacinus;
comparative example 3.3 is provided, which comparative example 3.3 differs from example 3 in that: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, Streptomyces griseus;
comparative example 3.4 is provided, which comparative example 3.4 differs from example 3 in that: does not add bacillus megatherium, radiorhizobium and paecilomyces lilacinus;
comparative example 3.5 is provided, which comparative example 3.5 differs from example 3 in that: bacillus megaterium, radiorhizobium and streptomyces griseus are not added;
comparative example 3.6 is provided, which comparative example 3.6 differs from example 3 in that: the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The complex microbial agents for ecological restoration prepared in comparative example 3.1, comparative example 3.2, comparative example 3.3, comparative example 3.4, comparative example 3.5, and comparative example 3.6 were observed for changes in physicochemical properties of soil, with the results being similar to those in comparative example 3 described above.
Further, the air conditioner is provided with a fan,
comparative example 4.1 is provided, which is distinguished from example 3 by the following: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, radiorhizobium, Streptomyces griseus;
comparative example 4.2 is provided, which is distinguished from example 3 by the following: bacillus megaterium, radiorhizobium, paecilomyces lilacinus and streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the physicochemical properties of the soil of the complex microbial agents for ecological restoration prepared in comparative examples 4.1 and 4.2 were observed, and the results were similar to those in comparative example 4 described above.
Further, the air conditioner is provided with a fan,
comparative example 5.1 is provided, which comparative example 5.1 differs from example 3 in that: replacing zeolite powder with sawdust;
comparative example 5.2 is provided, which comparative example 5.2 differs from example 3 in that: replacing magnesium sulfate with potassium sulfate;
comparative example 5.3 is provided, which comparative example 5.3 differs from example 3 in that: replacing cellulase with protease;
comparative example 5.4 is provided, which comparative example 5.4 differs from example 3 in that: replacing the wormcast with a nitrogen fertilizer;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the physicochemical properties of the soil by the complex microbial agents for ecological restoration prepared in comparative example 5.1, comparative example 5.2, comparative example 5.3, and comparative example 5.4 were observed, and the results were similar to those in comparative example 5 described above.
From the above results, it can be seen that:
the compound microbial agent for ecological restoration, which is provided by the invention, is added into saline-alkali soil, so that the cation exchange capacity of the soil can be obviously improved, the alkalization degree and the pH value of the saline-alkali soil can be obviously reduced, the physicochemical properties of the saline-alkali soil are obviously improved, and good physicochemical conditions are provided for the normal growth of plants; the effect of improving the cation exchange capacity of the soil is not obvious and the effect of reducing the alkalization degree and the pH value of the saline-alkali soil is also not obvious when the compound microbial agent for ecological restoration provided by the comparative example is added into the saline-alkali soil.
TABLE 2 changes in the biological Properties of the soil
Further, the air conditioner is provided with a fan,
comparative example 1.1 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium;
comparative example 1.2 is provided, which is distinguished from example 3 by the following: no radiorhizobium was added;
comparative example 1.3 is provided, which is distinguished from example 3 by the following: no paecilomyces lilacinus was added;
comparative example 1.4 is provided, which is distinguished from example 3 by the following: streptomyces griseus was not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the biological properties of the complex microbial agents for ecological restoration prepared in comparative example 1.1, comparative example 1.2, comparative example 1.3, and comparative example 1.4 were observed, and the results were similar to those in comparative example 1 described above.
Further, the air conditioner is provided with a fan,
comparative example 2.1 is provided, which is distinguished from example 3 by the following: no amylase streptomyces and radiorhizobium were added;
comparative example 2.2 is provided, which is distinguished from example 3 by the following: no thermal amylase chain mold and paecilomyces lilacinus added;
comparative example 2.3 is provided, which comparative example 2.3 differs from example 3 in that: no Thermoamylase Streptomyces and Streptomyces griseus were added;
comparative example 2.4 is provided, which comparative example 2.4 differs from example 3 in that: no Bacillus megaterium and radiorhizobium were added;
comparative example 2.5 is provided, which is distinguished from example 3 by the following: the bacillus megaterium and the paecilomyces lilacinus are not added;
comparative example 2.6 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium and Streptomyces griseus;
comparative example 2.7 is provided, which is distinguished from example 3 by the following: no radiorhizobium or paecilomyces lilacinus was added;
comparative example 2.8 is provided, which is distinguished from example 3 by the following: no radiorhizobium or streptomyces griseus added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the biological properties of the soil by the complex microbial agents for ecological restoration prepared in comparative example 2.1, comparative example 2.2, comparative example 2.3, comparative example 2.4, comparative example 2.5, comparative example 2.6, comparative example 2.7, and comparative example 2.8 were observed, and the results were similar to those in comparative example 2 described above.
Further, the air conditioner is provided with a fan,
comparative example 3.1 is provided, which comparative example 3.1 differs from example 3 in that: no amylase streptomyces and radiorhizobium were added;
comparative example 3.2 is provided, which comparative example 3.2 differs from example 3 in that: does not add thermal amylase chain mould, bacillus megaterium and paecilomyces lilacinus;
comparative example 3.3 is provided, which comparative example 3.3 differs from example 3 in that: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, Streptomyces griseus;
comparative example 3.4 is provided, which comparative example 3.4 differs from example 3 in that: does not add bacillus megatherium, radiorhizobium and paecilomyces lilacinus;
comparative example 3.5 is provided, which comparative example 3.5 differs from example 3 in that: bacillus megaterium, radiorhizobium and streptomyces griseus are not added;
comparative example 3.6 is provided, which comparative example 3.6 differs from example 3 in that: the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The compound microbial agents for ecological restoration prepared in comparative example 3.1, comparative example 3.2, comparative example 3.3, comparative example 3.4, comparative example 3.5, and comparative example 3.6 were observed for changes in biological properties of soil, with the results being similar to those in comparative example 3 described above.
Further, the air conditioner is provided with a fan,
comparative example 4.1 is provided, which is distinguished from example 3 by the following: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, radiorhizobium, Streptomyces griseus;
comparative example 4.2 is provided, which is distinguished from example 3 by the following: bacillus megaterium, radiorhizobium, paecilomyces lilacinus and streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the biological properties of the complex microbial agents for ecological restoration prepared in comparative examples 4.1 and 4.2 to the soil were observed, and the results were similar to those in comparative example 4 described above.
Further, the air conditioner is provided with a fan,
comparative example 5.1 is provided, which comparative example 5.1 differs from example 3 in that: replacing zeolite powder with sawdust;
comparative example 5.2 is provided, which comparative example 5.2 differs from example 3 in that: replacing magnesium sulfate with potassium sulfate;
comparative example 5.3 is provided, which comparative example 5.3 differs from example 3 in that: replacing cellulase with protease;
comparative example 5.4 is provided, which comparative example 5.4 differs from example 3 in that: replacing the wormcast with a nitrogen fertilizer;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The changes of the biological properties of the complex microbial agents for ecological restoration prepared in comparative example 5.1, comparative example 5.2, comparative example 5.3, and comparative example 5.4 to the soil were observed, and the results were similar to those in comparative example 5 described above.
From the above results, it can be seen that:
the compound microbial agent for ecological restoration, provided by the invention, is added into saline-alkali soil, so that the carbon content and nitrogen content of microorganisms in the soil can be obviously improved, and the dehydrogenase activity and alkaline phosphatase activity can be maintained at a higher level. After the weight parts of the components in the compound microbial agent for ecological restoration are further optimized, the compound microbial agent for ecological restoration can obviously improve the carbon content and the nitrogen content of microorganisms in soil, can obviously improve the activity of dehydrogenase and the activity of alkaline phosphatase, and maintains the two at a higher level. When the compound microbial agent for ecological restoration provided by the comparative example is added into saline-alkali soil, the effect of improving the content of microbial carbon and the content of microbial nitrogen in the soil is not obvious, and the maintaining effect on the activity of dehydrogenase and the activity of alkaline phosphatase is not ideal.
TABLE 3 growth conditions of maize
Further, the air conditioner is provided with a fan,
comparative example 1.1 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium;
comparative example 1.2 is provided, which is distinguished from example 3 by the following: no radiorhizobium was added;
comparative example 1.3 is provided, which is distinguished from example 3 by the following: no paecilomyces lilacinus was added;
comparative example 1.4 is provided, which is distinguished from example 3 by the following: streptomyces griseus was not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The effects of the complex microbial agents for bioremediation prepared in comparative example 1.1, comparative example 1.2, comparative example 1.3, and comparative example 1.4 on the growth conditions of corn were observed, and the results were similar to those in comparative example 1 described above.
Further, the air conditioner is provided with a fan,
comparative example 2.1 is provided, which is distinguished from example 3 by the following: no amylase streptomyces and radiorhizobium were added;
comparative example 2.2 is provided, which is distinguished from example 3 by the following: no thermal amylase chain mold and paecilomyces lilacinus added;
comparative example 2.3 is provided, which comparative example 2.3 differs from example 3 in that: no Thermoamylase Streptomyces and Streptomyces griseus were added;
comparative example 2.4 is provided, which comparative example 2.4 differs from example 3 in that: no Bacillus megaterium and radiorhizobium were added;
comparative example 2.5 is provided, which is distinguished from example 3 by the following: the bacillus megaterium and the paecilomyces lilacinus are not added;
comparative example 2.6 is provided, which is distinguished from example 3 by the following: no addition of Bacillus megaterium and Streptomyces griseus;
comparative example 2.7 is provided, which is distinguished from example 3 by the following: no radiorhizobium or paecilomyces lilacinus was added;
comparative example 2.8 is provided, which is distinguished from example 3 by the following: no radiorhizobium or streptomyces griseus added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The effects of the complex microbial agents for ecological restoration prepared in comparative example 2.1, comparative example 2.2, comparative example 2.3, comparative example 2.4, comparative example 2.5, comparative example 2.6, comparative example 2.7, and comparative example 2.8 on the growth conditions of corn were observed, and the results were similar to those in comparative example 2 described above.
Further, the air conditioner is provided with a fan,
comparative example 3.1 is provided, which comparative example 3.1 differs from example 3 in that: no amylase streptomyces and radiorhizobium were added;
comparative example 3.2 is provided, which comparative example 3.2 differs from example 3 in that: does not add thermal amylase chain mould, bacillus megaterium and paecilomyces lilacinus;
comparative example 3.3 is provided, which comparative example 3.3 differs from example 3 in that: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, Streptomyces griseus;
comparative example 3.4 is provided, which comparative example 3.4 differs from example 3 in that: does not add bacillus megatherium, radiorhizobium and paecilomyces lilacinus;
comparative example 3.5 is provided, which comparative example 3.5 differs from example 3 in that: bacillus megaterium, radiorhizobium and streptomyces griseus are not added;
comparative example 3.6 is provided, which comparative example 3.6 differs from example 3 in that: the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The effects of the complex microbial agents for ecological restoration prepared in comparative example 3.1, comparative example 3.2, comparative example 3.3, comparative example 3.4, comparative example 3.5, and comparative example 3.6 on the growth conditions of corn were observed, and the results were similar to those in comparative example 3 described above.
Further, the air conditioner is provided with a fan,
comparative example 4.1 is provided, which is distinguished from example 3 by the following: without the addition of Thermoamylase Streptomyces, Bacillus megaterium, radiorhizobium, Streptomyces griseus;
comparative example 4.2 is provided, which is distinguished from example 3 by the following: bacillus megaterium, radiorhizobium, paecilomyces lilacinus and streptomyces griseus are not added;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The effects of the complex microbial agents for ecological restoration prepared in comparative examples 4.1 and 4.2 on the growth conditions of corn were observed, and the results were similar to those in comparative example 4 described above.
Further, the air conditioner is provided with a fan,
comparative example 5.1 is provided, which comparative example 5.1 differs from example 3 in that: replacing zeolite powder with sawdust;
comparative example 5.2 is provided, which comparative example 5.2 differs from example 3 in that: replacing magnesium sulfate with potassium sulfate;
comparative example 5.3 is provided, which comparative example 5.3 differs from example 3 in that: replacing cellulase with protease;
comparative example 5.4 is provided, which comparative example 5.4 differs from example 3 in that: replacing the wormcast with a nitrogen fertilizer;
the compound microbial agent for ecological restoration is prepared by combining the preparation method of the invention, and experiments are carried out according to an experimental method for effect verification. The effects of the complex microbial agents for ecological restoration prepared in comparative example 5.1, comparative example 5.2, comparative example 5.3, and comparative example 5.4 on the growth conditions of corn were observed, and the results were similar to those in comparative example 5 described above.
From the above results, it can be seen that:
the corn seedlings treated by the composite microbial agent for ecological restoration provided by the invention have significant increases in root length, root surface area and root volume. When corn seedlings were treated with the complex microbial inoculant for ecological restoration provided in comparative example, the increase in root length, root surface area and root volume was not significant.
It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.
Claims (7)
1. The compound microbial agent for ecological restoration is characterized by comprising, by weight, 0.1-0.3 parts of thermal amylase streptomyces, 0.5-1 parts of bacillus megaterium, 0.1-0.5 parts of radiorhizobium, 1-3 parts of paecilomyces lilacinus and 0.5-2 parts of streptomyces griseus.
2. The complex microbial inoculant for ecological restoration according to claim 1, wherein the complex microbial inoculant for ecological restoration comprises thermal amylase streptomyces at a concentration of 10 hundred million cfu/g or more, bacillus megaterium at a concentration of 0.3-1 hundred million cfu/g, radiorhizobium at a concentration of 1-2 hundred million cfu/g, paecilomyces lilacinus at a concentration of 0.6-1 hundred million cfu/g, streptomyces griseus at a concentration of 0.6-1 hundred million cfu/g.
3. The complex microbial inoculant for ecological restoration according to claim 2, wherein the complex microbial inoculant for ecological restoration comprises 0.2 parts of thermal amylase streptomyces, 0.6 parts of bacillus megaterium, 0.3 parts of radiorhizobium, 2 parts of paecilomyces lilacinus and 1 part of streptomyces griseus according to parts by weight.
4. The complex microbial inoculant for ecological restoration according to claim 1, further comprising tartaric acid, zeolite powder, magnesium sulfate, cellulase and wormcast.
5. The compound microbial inoculant for ecological restoration according to claim 4, further comprising 10-15 parts by weight of tartaric acid, 5-8 parts by weight of zeolite powder, 10-20 parts by weight of magnesium sulfate, 13-15 parts by weight of cellulase and 25-30 parts by weight of wormcast.
6. The complex microbial inoculant for ecological restoration according to claim 5, further comprising 11 parts by weight of tartaric acid, 6 parts by weight of zeolite powder, 15 parts by weight of magnesium sulfate, 14 parts by weight of cellulase and 27 parts by weight of wormcast.
7. The method for preparing a complex microbial inoculant for ecological restoration according to claims 1-6, wherein the method comprises the following steps:
s1, firstly, mixing tartaric acid, zeolite powder and magnesium sulfate, then adding cellulase and wormcast, mixing again to obtain a mixed material, fermenting the mixed material for 5-8 days, sterilizing at 80-100 ℃ for 0.5-1h, and cooling to room temperature to obtain an adsorption carrier;
s2, sequentially adsorbing the thermal amylase streptomyces, the bacillus megatherium, the radiorhizobium, the paecilomyces lilacinus and the streptomyces griseus on the adsorption carrier obtained in the step S1, stirring, air-drying and crushing to obtain the compound microbial agent for ecological restoration.
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Application publication date: 20211102 |