CN115968401A - 将干细胞分化为多巴胺能祖细胞的方法 - Google Patents

将干细胞分化为多巴胺能祖细胞的方法 Download PDF

Info

Publication number
CN115968401A
CN115968401A CN202180044066.9A CN202180044066A CN115968401A CN 115968401 A CN115968401 A CN 115968401A CN 202180044066 A CN202180044066 A CN 202180044066A CN 115968401 A CN115968401 A CN 115968401A
Authority
CN
China
Prior art keywords
cells
signaling
stem cells
progenitor cells
midbrain dopaminergic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180044066.9A
Other languages
English (en)
Chinese (zh)
Inventor
L·J·埃里克森
Z·V·阿列克森科
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Simite Dopa Co ltd
Original Assignee
Jz Cell Technology Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jz Cell Technology Co filed Critical Jz Cell Technology Co
Publication of CN115968401A publication Critical patent/CN115968401A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0619Neurons
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/14Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16Anti-Parkinson drugs
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/119Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/13Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/385Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/41Hedgehog proteins; Cyclopamine (inhibitor)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00Use of cells in diagnostics
    • C12N2503/02Drug screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • C12N2533/52Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Neurology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurosurgery (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Psychology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202180044066.9A 2020-05-07 2021-05-07 将干细胞分化为多巴胺能祖细胞的方法 Pending CN115968401A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2006792.2 2020-05-07
GBGB2006792.2A GB202006792D0 (en) 2020-05-07 2020-05-07 Method and uses thereof
PCT/EP2021/062226 WO2021224496A1 (en) 2020-05-07 2021-05-07 Methods for differentiating stem cells into dopaminergic progenitor cells

Publications (1)

Publication Number Publication Date
CN115968401A true CN115968401A (zh) 2023-04-14

Family

ID=71134903

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180044066.9A Pending CN115968401A (zh) 2020-05-07 2021-05-07 将干细胞分化为多巴胺能祖细胞的方法

Country Status (7)

Country Link
US (1) US20230233617A1 (https=)
EP (1) EP4146796A1 (https=)
JP (1) JP2023525297A (https=)
CN (1) CN115968401A (https=)
CA (1) CA3182115A1 (https=)
GB (1) GB202006792D0 (https=)
WO (1) WO2021224496A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117448270A (zh) * 2023-12-22 2024-01-26 上海元戊医学技术有限公司 一种高效诱导多能干细胞分化为中脑多巴胺能神经前体细胞的方法
CN117660335A (zh) * 2023-12-14 2024-03-08 广州百康细胞生命科技有限公司 一种使用化学重编辑法将体细胞转化成免疫细胞的方法
WO2025026225A1 (zh) * 2023-07-28 2025-02-06 士泽生物医药(苏州)有限公司 一种诱导干细胞分化为运动神经前体细胞和/或运动神经元的试剂盒及其应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022081501A1 (en) * 2020-10-12 2022-04-21 Research Development Foundation Methods for making and using differentiated neural cells
AU2022247250A1 (en) 2021-03-30 2023-11-09 Trailhead Biosystems Inc. Methods and compositions for generating oligodendrocyte progenitor cells
JP2025524856A (ja) * 2022-07-21 2025-08-01 トレイルヘッド バイオシステムズ インコーポレイテッド 神経前駆細胞からヒト中脳ドーパミン作動性ニューロンを作製するための方法および組成物
CN115521903B (zh) * 2022-09-30 2024-05-17 中国科学院生态环境研究中心 体外诱导人多能干细胞分化为多巴胺能神经元的方法
US12565639B2 (en) 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
WO2025162486A1 (en) * 2024-01-31 2025-08-07 Center For Excellence In Brain Science And Intelligence Technology, Chinese Academy Of Sciences An additive composition and a method for promoting differentiation into a10 precursors and/or a10 neurons
WO2025193168A1 (en) * 2024-03-14 2025-09-18 National University Of Singapore Method of generating midbrain dopamine progenitors and neurons from stem cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101935635A (zh) * 2010-07-30 2011-01-05 中国人民解放军第二军医大学 人骨髓多能干细胞的体外扩增和向多巴胺能神经元定向分化的方法
US20130108669A1 (en) * 2010-04-16 2013-05-02 The Mclean Hospital Corporation Dopaminergic neurons differentiated from pluripotent stem cells and uses of thereof
CN104379731A (zh) * 2011-11-04 2015-02-25 纪念斯隆-凯特琳癌症中心 用于植入的中脑多巴胺(da)神经元
CN104379732A (zh) * 2012-04-24 2015-02-25 国际干细胞公司 由人多能干细胞衍生神经干细胞和多巴胺能神经元
US20180243343A1 (en) * 2015-02-26 2018-08-30 The Mclean Hospital Corporation Methods and compositions for the treatment or prevention of parkinson's disease

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001088104A2 (en) * 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
WO2010096496A2 (en) * 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización
US11767508B2 (en) * 2017-07-24 2023-09-26 Wisconsin Alumni Research Foundation Methods and culture substrates for controlled induction of biomimetic neural tissues comprising singular rosette structures

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130108669A1 (en) * 2010-04-16 2013-05-02 The Mclean Hospital Corporation Dopaminergic neurons differentiated from pluripotent stem cells and uses of thereof
CN101935635A (zh) * 2010-07-30 2011-01-05 中国人民解放军第二军医大学 人骨髓多能干细胞的体外扩增和向多巴胺能神经元定向分化的方法
CN104379731A (zh) * 2011-11-04 2015-02-25 纪念斯隆-凯特琳癌症中心 用于植入的中脑多巴胺(da)神经元
CN104379732A (zh) * 2012-04-24 2015-02-25 国际干细胞公司 由人多能干细胞衍生神经干细胞和多巴胺能神经元
US20180243343A1 (en) * 2015-02-26 2018-08-30 The Mclean Hospital Corporation Methods and compositions for the treatment or prevention of parkinson's disease

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
OLIVER COOPER等: "Differentiation of human ES and Parkinson\'s disease iPS cells into ventral midbrain dopaminergic neurons requires a high activity form of SHH, FGF8a and specific regionalization by retinoic acid", MOLECULAR AND CELLULAR NEUROSCIENCE, vol. 45, 24 July 2010 (2010-07-24), pages 1 *
潘宏旭等: "全反式维甲酸通过增强调节性T细胞的抑炎效应发挥对多巴胺能神经元的保护作用", 国际神经病学神经外科学杂志, 28 October 2017 (2017-10-28), pages 1 - 5 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025026225A1 (zh) * 2023-07-28 2025-02-06 士泽生物医药(苏州)有限公司 一种诱导干细胞分化为运动神经前体细胞和/或运动神经元的试剂盒及其应用
CN117660335A (zh) * 2023-12-14 2024-03-08 广州百康细胞生命科技有限公司 一种使用化学重编辑法将体细胞转化成免疫细胞的方法
CN117448270A (zh) * 2023-12-22 2024-01-26 上海元戊医学技术有限公司 一种高效诱导多能干细胞分化为中脑多巴胺能神经前体细胞的方法

Also Published As

Publication number Publication date
WO2021224496A1 (en) 2021-11-11
GB202006792D0 (en) 2020-06-24
JP2023525297A (ja) 2023-06-15
US20230233617A1 (en) 2023-07-27
CA3182115A1 (en) 2021-11-11
EP4146796A1 (en) 2023-03-15

Similar Documents

Publication Publication Date Title
JP7617066B2 (ja) 移植用中脳ドーパミン(da)ニューロン
US20230233617A1 (en) Methods for differentiating stem cells into dopaminergic progenitor cells
KR102625865B1 (ko) 만능 세포를 분화시키는 방법
US11406670B2 (en) Methods of generating glial and neuronal cells and use of same for the treatment of medical conditions of the CNS
JP5631210B2 (ja) 多能性幹細胞からのニューロン細胞の生成
WO2017005136A1 (en) Compositions and methods for reprograming non-neuronal cells into neuron-like cells
Addae et al. All-trans-retinoid acid induces the differentiation of encapsulated mouse embryonic stem cells into GABAergic neurons
KR20230165846A (ko) 도파민성 전구세포 및 사용 방법
CN101124319A (zh) 神经干细胞
Buzanska Human Neural Stem Cells: From Generation to Differentiation and Application
HK40090062A (zh) 将干细胞分化为多巴胺能祖细胞的方法
Bianco et al. Rapid serum-free isolation of oligodendrocyte progenitor cells from adult rat spinal cord
EP4735585A1 (en) Enhancing neuronal differentiation of neural progenitor cells
Abinaya Neural differentiation of mouse and human pluripotent stem cells

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40090062

Country of ref document: HK

TA01 Transfer of patent application right

Effective date of registration: 20250403

Address after: Swedish tullinge

Applicant after: Simite Dopa Co.,Ltd.

Country or region after: Sweden

Address before: Hasselby, Sweden

Applicant before: JZ Cell Technology Co.

Country or region before: Sweden

TA01 Transfer of patent application right