CA3182115A1 - Methods for differentiating stem cells into dopaminergic progenitor cells - Google Patents

Methods for differentiating stem cells into dopaminergic progenitor cells

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Publication number
CA3182115A1
CA3182115A1 CA3182115A CA3182115A CA3182115A1 CA 3182115 A1 CA3182115 A1 CA 3182115A1 CA 3182115 A CA3182115 A CA 3182115A CA 3182115 A CA3182115 A CA 3182115A CA 3182115 A1 CA3182115 A1 CA 3182115A1
Authority
CA
Canada
Prior art keywords
cells
signalling
stem cells
activator
neurons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3182115A
Other languages
English (en)
French (fr)
Inventor
Lars Johan ERICSON
Zjanna Vladimirovna ALEKSEENKO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jz Cell Technologies AB
Original Assignee
Jz Cell Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jz Cell Technologies AB filed Critical Jz Cell Technologies AB
Publication of CA3182115A1 publication Critical patent/CA3182115A1/en
Pending legal-status Critical Current

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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Neurology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurosurgery (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Psychology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA3182115A 2020-05-07 2021-05-07 Methods for differentiating stem cells into dopaminergic progenitor cells Pending CA3182115A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2006792.2 2020-05-07
GBGB2006792.2A GB202006792D0 (en) 2020-05-07 2020-05-07 Method and uses thereof
PCT/EP2021/062226 WO2021224496A1 (en) 2020-05-07 2021-05-07 Methods for differentiating stem cells into dopaminergic progenitor cells

Publications (1)

Publication Number Publication Date
CA3182115A1 true CA3182115A1 (en) 2021-11-11

Family

ID=71134903

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3182115A Pending CA3182115A1 (en) 2020-05-07 2021-05-07 Methods for differentiating stem cells into dopaminergic progenitor cells

Country Status (7)

Country Link
US (1) US20230233617A1 (https=)
EP (1) EP4146796A1 (https=)
JP (1) JP2023525297A (https=)
CN (1) CN115968401A (https=)
CA (1) CA3182115A1 (https=)
GB (1) GB202006792D0 (https=)
WO (1) WO2021224496A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022081501A1 (en) * 2020-10-12 2022-04-21 Research Development Foundation Methods for making and using differentiated neural cells
AU2022247250A1 (en) 2021-03-30 2023-11-09 Trailhead Biosystems Inc. Methods and compositions for generating oligodendrocyte progenitor cells
JP2025524856A (ja) * 2022-07-21 2025-08-01 トレイルヘッド バイオシステムズ インコーポレイテッド 神経前駆細胞からヒト中脳ドーパミン作動性ニューロンを作製するための方法および組成物
CN115521903B (zh) * 2022-09-30 2024-05-17 中国科学院生态环境研究中心 体外诱导人多能干细胞分化为多巴胺能神经元的方法
US12565639B2 (en) 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
CN119432743A (zh) * 2023-07-28 2025-02-14 士泽生物医药（苏州）有限公司 一种诱导干细胞分化为运动神经前体细胞和/或运动神经元的试剂盒及其应用
CN117660335A (zh) * 2023-12-14 2024-03-08 广州百康细胞生命科技有限公司 一种使用化学重编辑法将体细胞转化成免疫细胞的方法
CN117448270B (zh) * 2023-12-22 2024-07-26 上海元戊医学技术有限公司 一种高效诱导多能干细胞分化为中脑多巴胺能神经前体细胞的方法
WO2025162486A1 (en) * 2024-01-31 2025-08-07 Center For Excellence In Brain Science And Intelligence Technology, Chinese Academy Of Sciences An additive composition and a method for promoting differentiation into a10 precursors and/or a10 neurons
WO2025193168A1 (en) * 2024-03-14 2025-09-18 National University Of Singapore Method of generating midbrain dopamine progenitors and neurons from stem cells

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001088104A2 (en) * 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
WO2010096496A2 (en) * 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
WO2011130675A2 (en) * 2010-04-16 2011-10-20 The Mclean Hospital Corporation Dopaminergic neurons differentiated from pluripotent stem cells and uses of thereof
CN101935635A (zh) * 2010-07-30 2011-01-05 中国人民解放军第二军医大学 人骨髓多能干细胞的体外扩增和向多巴胺能神经元定向分化的方法
EP3693455A1 (en) * 2011-11-04 2020-08-12 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
WO2013163228A1 (en) * 2012-04-24 2013-10-31 International Stem Cell Corporation Derivation of neural stem cells and dopaminergic neurons from human pluripotent stem cells
ES2939807T3 (es) * 2014-03-21 2023-04-27 Fujifilm Cellular Dynamics Inc Producción de neuronas dopaminérgicas del mesencéfalo y métodos para su utilización
US20180243343A1 (en) * 2015-02-26 2018-08-30 The Mclean Hospital Corporation Methods and compositions for the treatment or prevention of parkinson's disease
US11767508B2 (en) * 2017-07-24 2023-09-26 Wisconsin Alumni Research Foundation Methods and culture substrates for controlled induction of biomimetic neural tissues comprising singular rosette structures

Also Published As

Publication number Publication date
WO2021224496A1 (en) 2021-11-11
CN115968401A (zh) 2023-04-14
GB202006792D0 (en) 2020-06-24
JP2023525297A (ja) 2023-06-15
US20230233617A1 (en) 2023-07-27
EP4146796A1 (en) 2023-03-15

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