CN115948223A - Full-automatic high-flux DNA extraction device - Google Patents
Full-automatic high-flux DNA extraction device Download PDFInfo
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- CN115948223A CN115948223A CN202310056072.7A CN202310056072A CN115948223A CN 115948223 A CN115948223 A CN 115948223A CN 202310056072 A CN202310056072 A CN 202310056072A CN 115948223 A CN115948223 A CN 115948223A
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Abstract
The invention discloses a full-automatic high-flux DNA extraction device, which relates to the technical field of DNA extraction devices and aims to solve the problem that extraction efficiency is affected because a shaking-up structure for heating or cooling the interior of some existing full-automatic high-flux DNA extraction equipment according to the extraction requirement of a sample does not exist in the device, so that the sample needs to be subjected to shaking-up treatment in another set of equipment before extraction. The effects of convenient use and stable work are achieved.
Description
Technical Field
The invention relates to the technical field of DNA extraction devices, in particular to a full-automatic high-throughput DNA extraction device.
Background
The DNA extractor generally refers to a nucleic acid purification instrument, is a high-tech product for extracting nucleic acid by using a general magnetic bead method, has the advantages of high automation degree, high extraction speed, stable result and simple and convenient operation, and can be matched with different types of magnetic bead nucleic acid reagent groups to quickly extract DNA and RNA in samples such as animal and plant tissues, blood, body fluids, criminal specimens and the like.
The above prior art solutions have the following drawbacks: some existing full-automatic high-throughput DNA extraction equipment is not internally provided with a shaking structure for heating or cooling the extraction requirement of a sample, so that the sample is extracted after being shaken up by another group of equipment before extraction, and the extraction efficiency is influenced.
Disclosure of Invention
The invention aims to provide a full-automatic high-throughput DNA extraction device which can heat or cool a sample.
In order to achieve the purpose, the invention provides the following technical scheme:
the full-automatic high-flux DNA extraction device comprises a supporting table, wherein the upper end of the supporting table is fixedly connected with a protective shell and an installation box, the interior of the installation box is fixedly connected with a shaking component, the upper end of the shaking component is fixedly connected with a placing box, and the interior of the placing box is fixedly connected with a heating plate and a condensing plate;
rock the inside of subassembly including first motor, cam, connecting block, movable rod, spacing post and fixed plate, the output fixedly connected with cam of first motor, the outside swing joint of cam has the connecting block, the outside swing joint of connecting block has the movable rod, the spacing post of inside fixedly connected with of install bin, the outside fixedly connected with fixed plate of movable rod.
Through adopting above-mentioned technical scheme, increase in the inside of equipment and can drive the sample and carry out the structure of rocking, increase hot plate and condenser plate in the inside of placing the box simultaneously for can heat or cool down the sample, with satisfying the demand of different samples to extraction temperature, some need shake even sample, can go on in step under these two kinds of functions, improve the efficiency of extracting, it is more convenient to use.
Further, rotate through the pivot between the one end in the connecting block outside and the cam and be connected, rotate through the pivot between the other end in the connecting block outside and the movable rod and be connected, spacing post cup joints the outside at the movable rod, the movable rod slides in the inside of spacing post, the fixed plate with place box fixed connection.
Through adopting above-mentioned technical scheme, drive the cam through first motor and carry out the transmission for the cam can drive connecting block and movable rod and carry out the transmission simultaneously in the driven, and the outside of movable rod receives the limiting displacement of spacing post simultaneously, makes the movable rod can carry out linear motion, and it rocks to drive the sample box with this.
Further, the upper end of the placing box is fixedly connected with a placing plate, the outer side of the placing plate is fixedly connected with a temperature sensor, a placing groove and a cavity are formed in the placing plate, a moving block and a spring are arranged in the cavity, the outer side of the moving block is located on the clamping plate fixedly connected with the inner side of the placing groove, and a sample box is arranged in the placing groove and located between the clamping plates.
Through adopting above-mentioned technical scheme, can the centre gripping more stable in order to guarantee the sample box, the operation of conveniently better drawing for the grip block can carry out the centre gripping with the sample box through the effect of spring and fix, and the convenience of taking simultaneously, the operation of being convenient for through the flexible effect between movable block and the spring.
Furthermore, the placing groove, the cavity, the moving block, the springs and the clamping plates are respectively provided with three groups of moving blocks and two clamping plates at equal intervals, the springs in each group are arranged at equal intervals, one end of each spring is fixedly connected inside the cavity, the other end of each spring is fixedly connected with the moving block, and the moving blocks are movably connected with the placing plates through the springs.
Through adopting above-mentioned technical scheme, through setting up multiunit spring and grip block, can put a plurality of sample boxes and place the board, improve the efficiency of drawing.
Further, the inside fixedly connected with first moving mechanism of brace table, the upper end fixedly connected with support post of first moving mechanism, fixedly connected with second moving mechanism between the support post, the outside fixedly connected with electric telescopic handle of second moving mechanism, electric telescopic handle's flexible end fixedly connected with fixing base, the lower extreme fixedly connected with high flux of fixing base moves the liquid head.
Through adopting above-mentioned technical scheme, realize longitudinal movement through first moving mechanism, second moving mechanism realizes lateral shifting simultaneously, realizes the removal regulation of vertical direction through electric telescopic handle simultaneously, can drive the high flux and move the liquid head and carry out the removal regulation of three direction, the convenient better operation of drawing.
Further, the inside of first moving mechanism is including first mount pad, second motor, first lead screw and first movable block, the outside fixedly connected with second motor of first mount pad, the inside of first mount pad is provided with first lead screw, the outside of first lead screw is provided with first movable block, first moving mechanism symmetry is provided with two sets ofly, the transmission end of second motor is connected with the one end transmission of first lead screw, rotate through the bearing between the other end of first lead screw and the first mount pad and be connected, first lead screw and first movable block threaded connection, first movable block and support column fixed connection, the support column symmetry is provided with two.
Through adopting above-mentioned technical scheme, drive first lead screw through the second motor and carry out the transmission for rotate between first lead screw and the first mount pad, the first movable block that is located the first lead screw outside simultaneously begins to remove along the screw thread in the first lead screw outside, with this vertical removal of realization, convenient regulation.
Further, the inside of second moving mechanism is including connecting plate, gag lever post, electronic slide rail, electronic slider and mounting panel, the outside fixedly connected with gag lever post and the electronic slide rail of connecting plate, the outside sliding connection of electronic slide rail has electronic slider, electronic slider fixed mounting is in the outside of mounting panel, connecting plate fixed mounting is between the support post, the gag lever post pass the mounting panel, and with sliding connection between the mounting panel.
Through adopting above-mentioned technical scheme, drive electronic slider through electronic slide rail and remove to this realizes lateral shifting, and the gag lever post can improve the stability of lateral shifting in-process structure simultaneously.
Further, the outside fixedly connected with control panel of protecting crust, the upper end of brace table is located the outside fixedly connected with storage box of protecting crust, the inside fixedly connected with of storage box places the platform, the inner wall fixedly connected with ultraviolet ray disinfection lamp of storage box, the ultraviolet ray disinfection lamp symmetry is provided with two sets ofly, every group ultraviolet ray disinfection lamp equidistant is provided with a plurality ofly.
Through adopting above-mentioned technical scheme, increase a set of storage box in the upper end of brace table, can deposit the sample of waiting to draw, avoid the sample to be infected by the bacterium simultaneously, disinfect the disinfection through ultraviolet ray disinfection lamp to the case inside, convenient better use.
In conclusion, the beneficial technical effects of the invention are as follows:
1. the rocking component, the heating plate and the condensing plate are adopted, the motor in the rocking component drives the cam to transmit, meanwhile, the cam drives the connecting block and the movable rod to transmit, then, the movable rod drives the placing plate to perform reciprocating linear motion under the action of the limiting column, so that the rocking function is realized, and meanwhile, the heating plate and the condensing plate can heat or cool samples placed on the placing plate, so that the requirements of different samples on extraction temperature are met, and the effect of convenient use is achieved;
2. the first moving mechanism, the second moving mechanism and the electric telescopic rod are adopted, the longitudinal movement of the high-flux liquid transferring head is realized through the first moving mechanism, the transverse movement of the high-flux liquid transferring head is realized through the second moving mechanism, and the vertical movement is realized through the electric telescopic rod, so that the position adjustment is facilitated and better, the extraction operation is facilitated and the stable working effect is generated;
3. adopted ultraviolet ray disinfection lamp and storage box, through the next door increase storage box at the extraction equipment, can deposit the sample of waiting to draw temporarily, disinfect the disinfection through ultraviolet ray disinfection lamp to the case inside, avoid the sample to be infected by the bacterium, make things convenient for the use in later stage.
Drawings
FIG. 1 is a first general structural diagram of the present invention;
FIG. 2 is a schematic view of the overall structure of the present invention;
FIG. 3 is a schematic perspective view of a first moving mechanism and a second moving mechanism according to the present invention;
FIG. 4 is a schematic view of a split structure according to the present invention;
FIG. 5 is a schematic perspective view of a wobble assembly of the present invention;
FIG. 6 is a schematic cross-sectional view of the placement board of the present invention;
FIG. 7 is an enlarged view of the structure at A in FIG. 6 according to the present invention.
In the figure, 1, a support table; 2. a protective shell; 3. installing a box; 4. a wobble assembly; 5. a temperature sensor; 6. placing the box; 7. heating the plate; 8. a condensing plate; 9. placing a plate; 10. a placement groove; 11. a cavity; 12. a moving block; 13. a spring; 14. a clamping plate; 15. a sample cartridge; 16. a first moving mechanism; 17. a support column; 18. a second moving mechanism; 19. an electric telescopic rod; 20. a fixed seat; 21. a high-throughput pipetting head; 22. a control panel; 23. a storage box; 24. a placing table; 25. an ultraviolet disinfection lamp; 41. a first motor; 42. a cam; 43. connecting blocks; 44. a movable rod; 45. a limiting column; 46. a fixing plate; 161. a first mounting seat; 162. a second motor; 163. a first lead screw; 164. a first movable block; 181. a connecting plate; 182. a limiting rod; 183. an electric slide rail; 184. an electric slider; 185. and (7) mounting the plate.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Referring to fig. 1-7, a full-automatic high-flux DNA extraction device, including a support table 1, a protective shell 2 and an installation box 3 are fixedly connected to the upper end of the support table 1, a shaking assembly 4 is fixedly connected to the interior of the installation box 3, the interior of the shaking assembly 4 includes a first motor 41, a cam 42, a connecting block 43, a movable rod 44, a limit post 45 and a fixed plate 46, the output end of the first motor 41 is fixedly connected with the cam 42, the outer side of the cam 42 is movably connected with the connecting block 43, the outer side of the connecting block 43 is movably connected with the movable rod 44, the interior of the installation box 3 is fixedly connected with the limit post 45, the outer side of the movable rod 44 is fixedly connected with the fixed plate 46, the upper end of the shaking assembly 4 is fixedly connected with a placing box 6, the interior of the placing box 6 is fixedly connected with a heating plate 7 and a condensation plate 8, one end of the outer side of the connecting block 43 is rotatably connected with the cam 42 through a rotating shaft, the other end of the outer side of the connecting block 43 is rotatably connected with the movable rod 44 through a rotating shaft, the limit post 45 is sleeved on the outer side of the movable rod 44, the movable rod 44 is sleeved on the outer side of the fixed plate 45, the fixed plate 44 slides inside of the limit post 45, the fixed plate 46 is fixedly connected with the placing box 6, the fixing plate 22 is fixedly connected with the temperature sensor 5, the ultraviolet radiation sensor 7, the heating plate 19, the heating plate 184, the electric motor is connected with the electric slide rail 183, the electric expansion rod 162 and the electric expansion rod 162, a structure which can drive the sample to shake is added in the device, a heating plate 7 and a condensing plate 8 are added in the placing box 6, so that the sample can be heated or cooled to meet the requirements of different samples on extraction temperature, and some samples need to be shaken up, can be synchronously carried out under the two functions, improves the extraction efficiency and is more convenient to use.
As shown in fig. 2-5, a placing plate 9 is fixedly connected to the upper end of the placing box 6, a temperature sensor 5 is fixedly connected to the outer side of the placing plate 9, a placing groove 10 and a cavity 11 are formed in the placing plate 9, a moving block 12 and a spring 13 are arranged in the cavity 11, a clamping plate 14 is fixedly connected to the outer side of the moving block 12 in the placing groove 10, a sample box 15 is arranged between the clamping plates 14 in the placing groove 10, three groups of the placing groove 10, the cavity 11, the moving block 12, the spring 13 and the clamping plate 14 are respectively arranged at equal intervals, two groups of the moving block 12 and the clamping plate 14 are arranged, a plurality of springs 13 are arranged at equal intervals in each group, one end of each spring 13 is fixedly connected to the inside of the cavity 11, the other end of each spring 13 is fixedly connected to the moving block 12, and the moving block 12 and the placing plate 9 are movably connected through the springs 13.
As shown in fig. 2-4, a first moving mechanism 16 is fixedly connected inside the supporting table 1, a supporting column 17 is fixedly connected at the upper end of the first moving mechanism 16, a second moving mechanism 18 is fixedly connected between the supporting columns 17, an electric telescopic rod 19 is fixedly connected outside the second moving mechanism 18, a fixed seat 20 is fixedly connected at the telescopic end of the electric telescopic rod 19, a high-flux liquid-transferring head 21 is fixedly connected at the lower end of the fixed seat 20, a first mounting seat 161, a second motor 162, a first screw rod 163 and a first movable block 164 are arranged inside the first moving mechanism 16, a second motor 162 is fixedly connected outside the first mounting seat 161, a first screw rod 163 is arranged inside the first mounting seat 161, a first movable block 164 is arranged outside the first screw rod 163, two sets of first moving mechanisms 16 are symmetrically arranged, a transmission end of the second motor 162 is in transmission connection with one end of the first screw rod 163, the other end of the first screw 163 is rotatably connected with the first mounting base 161 through a bearing, the first screw 163 is in threaded connection with the first movable block 164, the first movable block 164 is fixedly connected with the support column 17, two support columns 17 are symmetrically arranged, the second moving mechanism 18 comprises a connecting plate 181, a limiting rod 182, an electric slide rail 183, an electric slide block 184 and a mounting plate 185 inside, the limiting rod 182 and the electric slide rail 183 are fixedly connected with the outer side of the connecting plate 181, the electric slide block 184 is slidably connected with the outer side of the electric slide rail 183, the electric slide block 184 is fixedly mounted on the outer side of the mounting plate 185, the connecting plate 181 is fixedly mounted between the support columns 17, the limiting rod 182 penetrates through the mounting plate 185 and is slidably connected with the mounting plate 185, the outer side of the protective shell 2 is fixedly connected with a control panel 22, the upper end of the support table 1 is located at the outer side of the protective shell 2 and is fixedly connected with a storage box 23, platform 24 is placed to the inside fixedly connected with of storage box 23, the inner wall fixedly connected with ultraviolet ray disinfection lamp 25 of storage box 23, ultraviolet ray disinfection lamp 25 symmetry is provided with two sets ofly, every group ultraviolet ray disinfection lamp 25 equidistant is provided with a plurality ofly, realize longitudinal movement through first moving mechanism 16, second moving mechanism 18 realizes lateral shifting simultaneously, the removal through electric telescopic handle 19 realization vertical direction is adjusted simultaneously, can drive high flux liquid transfer head 21 and carry out the removal regulation of three direction, the convenient better operation that draws.
The implementation principle of the embodiment is as follows: when extracting, can take out sample box 15 from storage box 23, later put sample box 15 in standing groove 10 of placing board 9 inside, through the flexible effect between grip block 14 and spring 13, it is fixed to carry out the centre gripping with sample box 15, before extracting, some samples extract and need shake evenly, or need heating and cooling, with this needs that satisfy different needs, can heat or cool down through hot plate 7 and condenser plate 8, temperature sensor 5 detects the temperature change simultaneously, shake even, drive cam 42 through first motor 41 and carry out the transmission, make cam 42 can drive connecting block 43 and movable rod 44 and carry out the transmission simultaneously in the transmission, the outside of movable rod 44 receives the limiting displacement of spacing post 45 simultaneously, make movable rod 44 can carry out linear motion, so that this drives sample box 15 and rocks, later begin to extract, drive first lead screw 163 through second motor 162 and carry out the transmission, make rotate between first lead screw 163 and the first mount pad 161, first movable block 164 that lies in the outside of first lead screw 163 begins to move along the screw thread of first movable block 163 and shake, it drives to carry out the transmission through the horizontal movement of slider 183 to realize that this high flux moves when this high-adjustable slide rail moves, it can realize that the horizontal movement of the slider 183 to carry out the horizontal movement of this high-speed adjustment of the high-rate liquid removal of the slider structure, it can carry out the horizontal movement of the high-adjustable liquid removal of this high-rate of the electric slider, it can be realized that the slider 21 in the high-adjustable slide rail structure, it can carry out the horizontal movement of this lead screw 163, it is convenient to carry out the horizontal movement of this lead screw to carry out the horizontal movement of the high-moving of the high-speed liquid extraction, it can be convenient to carry out the horizontal movement of the operation of the slider, it.
The embodiments of the present invention are all preferred embodiments of the present invention, and the scope of the present invention is not limited thereby, so: equivalent changes made according to the structure, shape and principle of the invention shall be covered by the protection scope of the invention.
Claims (8)
1. Full-automatic high flux DNA extraction element, including brace table (1), its characterized in that: the upper end of the supporting table (1) is fixedly connected with a protective shell (2) and an installation box (3), the interior of the installation box (3) is fixedly connected with a shaking assembly (4), the upper end of the shaking assembly (4) is fixedly connected with a placing box (6), and the interior of the placing box (6) is fixedly connected with a heating plate (7) and a condensing plate (8);
the utility model discloses a rocking device, including rock the inside of subassembly (4) including first motor (41), cam (42), connecting block (43), movable rod (44), spacing post (45) and fixed plate (46), the output fixedly connected with cam (42) of first motor (41), the outside swing joint of cam (42) has connecting block (43), the outside swing joint of connecting block (43) has movable rod (44), the inside fixedly connected with spacing post (45) of install bin (3), the outside fixedly connected with fixed plate (46) of movable rod (44).
2. The full-automatic high-throughput DNA extraction device according to claim 1, characterized in that: rotate through the pivot between one end in connecting block (43) outside and cam (42) and be connected, rotate through the pivot between the other end in connecting block (43) outside and movable rod (44) and be connected, spacing post (45) cup joint the outside at movable rod (44), movable rod (44) slide in the inside of spacing post (45), fixed plate (46) with place box (6) fixed connection.
3. The full-automatic high-throughput DNA extraction device according to claim 1, characterized in that: the temperature measuring device is characterized in that a placing plate (9) is fixedly connected to the upper end of the placing box (6), a temperature sensor (5) is fixedly connected to the outer side of the placing plate (9), a placing groove (10) and a cavity (11) are formed in the placing plate (9), a moving block (12) and a spring (13) are arranged in the cavity (11), a clamping plate (14) is fixedly connected to the inner side of the placing groove (10) and located on the outer side of the moving block (12), and a sample box (15) is arranged between the clamping plates (14) and located on the inner side of the placing groove (10).
4. The full-automatic high-throughput DNA extraction device according to claim 3, characterized in that: the movable type parking device is characterized in that three groups of placing grooves (10), cavities (11), moving blocks (12), springs (13) and clamping plates (14) are respectively arranged at equal intervals, two groups of moving blocks (12) and two groups of clamping plates (14) are arranged in each group, a plurality of springs (13) are arranged at equal intervals in each group, one ends of the springs (13) are fixedly connected inside the cavities (11), the other ends of the springs (13) are fixedly connected with the moving blocks (12), and the moving blocks (12) are movably connected with the placing plates (9) through the springs (13).
5. The full-automatic high-throughput DNA extraction device according to claim 1, characterized in that: the utility model discloses a liquid-removing device, including brace table (1), the first moving mechanism of inside fixedly connected with (16) of brace table (1), the upper end fixedly connected with support post (17) of first moving mechanism (16), fixedly connected with second moving mechanism (18) between support post (17), the outside fixedly connected with electric telescopic handle (19) of second moving mechanism (18), the flexible end fixedly connected with fixing base (20) of electric telescopic handle (19), the lower extreme fixedly connected with high flux of fixing base (20) moves liquid head (21).
6. The full-automatic high-throughput DNA extraction device according to claim 5, characterized in that: the inside of first moving mechanism (16) is including first mount pad (161), second motor (162), first lead screw (163) and first movable block (164), the outside fixedly connected with second motor (162) of first mount pad (161), the inside of first mount pad (161) is provided with first lead screw (163), the outside of first lead screw (163) is provided with first movable block (164), first moving mechanism (16) symmetry is provided with two sets ofly, the transmission end of second motor (162) is connected with the one end transmission of first lead screw (163), rotate through the bearing between the other end of first lead screw (163) and first mount pad (161) and be connected, first lead screw (163) and first movable block (164) threaded connection, first movable block (164) and support post (17) fixed connection, support post (17) symmetry is provided with two.
7. The full-automatic high-throughput DNA extraction device according to claim 5, characterized in that: the inside of second moving mechanism (18) is including connecting plate (181), gag lever post (182), electronic slide rail (183), electronic slider (184) and mounting panel (185), the outside fixedly connected with gag lever post (182) and electronic slide rail (183) of connecting plate (181), the outside sliding connection of electronic slide rail (183) has electronic slider (184), electronic slider (184) fixed mounting is in the outside of mounting panel (185), connecting plate (181) fixed mounting is between support post (17), mounting panel (185) are passed in gag lever post (182), and with sliding connection between mounting panel (185).
8. The full-automatic high-throughput DNA extraction device according to claim 1, characterized in that: the outside fixedly connected with control panel (22) of protecting crust (2), the upper end of brace table (1) is located outside fixedly connected with storage box (23) of protecting crust (2), platform (24) are placed to the inside fixedly connected with of storage box (23), inner wall fixedly connected with ultraviolet ray disinfection lamp (25) of storage box (23), ultraviolet ray disinfection lamp (25) symmetry is provided with two sets ofly, every group ultraviolet ray disinfection lamp (25) equidistant a plurality of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310056072.7A CN115948223A (en) | 2023-01-18 | 2023-01-18 | Full-automatic high-flux DNA extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310056072.7A CN115948223A (en) | 2023-01-18 | 2023-01-18 | Full-automatic high-flux DNA extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115948223A true CN115948223A (en) | 2023-04-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310056072.7A Withdrawn CN115948223A (en) | 2023-01-18 | 2023-01-18 | Full-automatic high-flux DNA extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115948223A (en) |
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2023
- 2023-01-18 CN CN202310056072.7A patent/CN115948223A/en not_active Withdrawn
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Application publication date: 20230411 |
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