CN115944571A

CN115944571A - Essence spray for promoting hair growth and repairing hair roots and preparation method thereof

Info

Publication number: CN115944571A
Application number: CN202310033910.9A
Authority: CN
Inventors: 徐兰举; 刘鑫; 齐磊; 李华; 于月欣
Original assignee: Hebei Naco Biotechnology Co ltd
Current assignee: Hebei Naco Biotechnology Co ltd
Priority date: 2023-01-10
Filing date: 2023-01-10
Publication date: 2023-04-11
Anticipated expiration: 2043-01-10
Also published as: CN115944571B

Abstract

The invention relates to an essence spray for promoting hair growth and repairing hair roots and a preparation method thereof, wherein the essence spray comprises a solvent and freeze-dried powder: the solvent comprises: glycerol, 1, 3-butanediol, 1, 2-hexanediol, an extracting solution of swertia japonica, an extracting solution of green tea, rosemary leaf oil, mint essential oil and the balance of ultrapure water; the freeze-dried powder comprises: the SOD superoxide dismutase, the recombinant collagen and the trehalose are stable in a long-term biological active component at room temperature, do not generate drug resistance and drug resistance when curing diseases, are safe and effective, do not generate toxic and side effects on human bodies, establish a healthy scalp ecological environment, delay hair falling, and repair hair follicle cells of the scalp, thereby having the effects of hair strengthening, inflammation diminishing and hair growing and fundamentally solving the problem of hair loss.

Description

Essence spray for promoting hair growth and repairing hair roots and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an essence spray for promoting hair growth and repairing hair roots and a preparation method thereof.

Background

Alopecia refers to a state where there is no hair or little hair at a site where hair should be present, and is classified as a progressive disease induced by an unsuccessful progress of nutrition supply and metabolism required for hair growth. With the increase of life rhythm and working pressure, more and more people can suffer from alopecia. According to the investigation of the alopecia population released by Weijian Wei in 2021, about 2.5 hundred million alopecia population accounts for nearly 1/5 of the total population in China. Alopecia is an important civil health problem, not only affects the beauty of patients, but also brings great mental and psychological burden to the patients. Scalp hair health care which mainly requires hair loss prevention is a basic requirement of consumers. At present, the clinically common methods for improving alopecia are mainly medicinal and surgical treatment, but have obvious limitations and disadvantages. Oral and external medicines, such as minoxidil, oral finasteride and the like can temporarily relieve alopecia symptoms, but have the problems of great side effect, unstable curative effect, repeated disease conditions after stopping taking the medicine and the like. Surgical treatment mostly adopts autologous single hair follicles and follicular units for transplantation, and has the disadvantages of limited number, unstable survival rate and high cost. Therefore, the search for new safe and effective ways to treat alopecia is particularly important.

The safety of a plurality of existing pilatory in the market, including chemical pilatory and Chinese herbal medicine pilatory, is questionable, and the Chinese herbal medicine pilatory is various in variety and inconsistent in effect. In addition, the existing pilatory is usually lotion or liniment, so that the use is inconvenient, the retention time of the effective components on the affected part is short, the actual curative effect is not obvious, and the treatment of patients is difficult.

In view of the above, it is important to provide a bioactive preparation with stable properties and excellent efficacy for treating alopecia and repairing hair roots.

Disclosure of Invention

The invention aims to overcome the defects in the prior art, provides the essence spray which is reasonable in compatibility, convenient to use, safe and comfortable, and can effectively promote hair growth and repair hair roots, and the preparation method thereof.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides an essence spray for promoting hair growth and repairing hair roots, which comprises a solvent and freeze-dried powder;

the menstruum comprises the following components in percentage by mass: 3 to 10 percent of glycerol, 0.1 to 10 percent of 1, 3-butanediol, 0.5 to 5 percent of 1, 2-hexanediol, 2 to 4 percent of swertia japonica extract, 0.1 to 2 percent of green tea extract, 0.3 to 0.5 percent of rosemary leaf oil, 0.1 to 0.3 percent of mint essential oil and the balance of ultrapure water;

the freeze-dried powder comprises the following components in percentage by mass and volume with a solvent: 1 mg-5 mg/20mL SOD, 10 mg-50 mg/20mL recombinant collagen, 0.2 g-1 g/20mL trehalose.

As some preferred embodiments of the invention, the molecular weight of the SOD superoxide dismutase is 10-30 kDa, and the specific activity of the SOD superoxide dismutase is 10000-30000U/mg.

As some preferred embodiments of the present invention, the molecular weight of the recombinant collagen is in the range of 25kDa to 90kDa.

As some preferred embodiments of the invention, the SOD superoxide dismutase and the recombinant collagen are obtained by gene recombination technology and microbial fermentation.

As some preferred embodiments of the present invention, the essence spray consists of a vehicle and a lyophilized powder:

the solvent comprises the following components in percentage by mass: 5% of glycerin, 3% of 1, 3-butanediol, 1% of 1, 2-hexanediol, 3% of a Japanese swertia herb extract, 0.5% of a green tea extract, 0.3% of rosemary leaf oil, 0.1% of mint essential oil and the balance of ultrapure water;

the freeze-dried powder comprises the following components in percentage by mass and volume with the solvent: SOD superoxide dismutase 2 mg-4 mg/20mL, recombinant collagen 10 mg-20 mg/20mL, trehalose 0.4g/20mL.

The invention also provides a preparation method of the essence spray for promoting hair growth and repairing hair roots, which specifically comprises the steps of preparing a solvent and preparing freeze-dried powder.

As some preferred embodiments of the present invention, the solvent is prepared as follows:

(1) Adding the extractive solution of swertia japonica Makino, the extractive solution of green tea, the leaf oil of Rosmarinus officinalis, the essential oil of herba Menthae, glycerol, 1, 2-hexanediol and 1, 3-butanediol into ultrapure water, adding the balance of ultrapure water, and stirring;

(2) Filtering the mixed solution with a 0.45-micron filter membrane, and subpackaging to obtain the solution of the essence spray. .

As some preferred embodiments of the present invention, the preparation method of the lyophilized powder is as follows:

s1, dissolving trehalose in ultrapure water to obtain a phase a;

s2, filtering and sterilizing the SOD superoxide dismutase stock solution and the recombinant collagen stock solution by using a 0.22-micron microporous filter membrane respectively, adding the sterilized SOD superoxide dismutase stock solution and the recombinant collagen stock solution into the phase a, and uniformly mixing;

and S3, placing the obtained mixed solution in a container, and freeze-drying to obtain freeze-dried powder. (ii) a

As some preferred embodiments of the invention, the method further comprises the step of subpackaging and combining the solvent and the lyophilized powder by using dry and wet mother-son bottles.

The invention further provides a use method of the essence spray for promoting hair growth and repairing hair roots, which comprises the following specific steps: pressing several pump heads forcibly to pump the solvent into the upper layer of lyophilized powder, and shaking to dissolve the lyophilized powder; after mixing, the essence is sprayed directly to the hair root of the head, and the part with hair skin exposed is sprayed more, and the hair is massaged by hand to complete absorption once a day.

Adopt the produced beneficial effect of above-mentioned technical scheme to lie in:

the essence spray for promoting hair growth and repairing hair roots provided by the invention contains SOD superoxide dismutase, recombinant collagen and four kinds of hair growing plant essence which are mutually cooperated, has the effects of removing free radicals, repairing damaged hair follicle cells, promoting hair regeneration, deeply nourishing scalp hair roots and the like, and can improve alopecia, resist scalp aging and maintain healthy scalp microecology.

The composition is provided by two components of solution and freeze-dried powder, and the active ingredients of SOD superoxide dismutase and recombinant collagen are provided in a freeze-dried product form, so that the biological activity of the composition is maintained for a long time, and the effects of the active ingredients can be synergistically exerted after compounding. The SOD superoxide dismutase has super oxidation resistance, effectively removes vivotoxin and free radicals, recovers the activity of damaged cells, promotes the blood circulation of scalp, improves hair quality, and enables hair to be regenerated and black and bright; the recombinant collagen has strong permeability to scalp, can participate in improving metabolism of skin cells, can stimulate differentiation of hair follicle stem cells, increase hair follicle number, and stimulate transformation of hair follicles in resting stage to growth stage, thereby preventing alopecia. It is reported in the literature that collagen is also indispensable in the process of producing transforming growth factor-beta from hair follicle stem cells, and the transforming growth factor-beta plays an important role in the production of pigment stem cells. Therefore, the collagen can effectively improve the problems of alopecia, white hair and the like, and the protein supplements the hair core, so that the hair is stronger and the hair is prevented from being dried and broken. Because of the synergistic effect of the SOD superoxide dismutase and the recombinant collagen component, the hair-care product can repair and nourish hair roots, strengthen the hair roots and prevent alopecia more effectively. In addition, the swertia japonica extract has effects of promoting hair growth by expanding peripheral blood vessels, promoting blood circulation, and increasing skin temperature. The tea polyphenol in the green tea extract can obviously reduce cyclophosphamide-induced alopecia and promote hair regeneration. The preferred multiple components of the hair loss preventing and conditioning agent have synergistic effect, the hair loss preventing and conditioning effects are remarkable, and irritation caused by the traditional chemical hair restorer is avoided.

The essence spray disclosed by the invention has the advantages that the bioactive components are stable for a long time at room temperature, the essence spray can not generate drug resistance and drug resistance while curing diseases, is safe and effective, can not generate toxic and side effects on a human body, establishes a healthy scalp ecological environment, delays hair falling, and repairs hair follicle cells, so that the effects of hair strengthening, inflammation diminishing and hair growing are achieved, and the problem of hair loss is fundamentally solved.

Drawings

In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below.

FIG. 1 is an electrophoresis chart comparing the stability of the essence spray of example 1 with that of the essence spray of comparative example 1 for 12 months; m-protein molecular weight Marker, 1-spraying the essence in example 1 for 0 day, 2-spraying the essence in comparative example 1 for 0 day, 3-spraying the essence in example 1 for 12 months at room temperature, and 4-spraying the essence in comparative example 1 for 12 months at room temperature.

FIG. 2 is a graph showing the comparison of the effects of the present invention in example 1.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the following description of the present invention is given for clarity and completeness.

In this embodiment, both the SOD superoxide dismutase and the recombinant collagen are obtained by gene recombination technology and microbial fermentation. The recombinant collagen is prepared by adopting a method disclosed in Chinese patent 201911384531.4 and an invention patent named as 'a recombinant humanized III-type collagen with a functional structure and an expression method thereof'. The dry and wet master bottles are commercially available products and are used for subpackaging the solution and the dry powder and mixing the solution and the dry powder in a dry and wet mode when in use.

Example 1

The composition of the essence spray for promoting hair growth and repairing hair roots of this example was as follows (% represents mass percent):

the preparation method of the solvent comprises the following steps:

(2) Filtering the mixed solution with a 0.45-micron filter membrane, and subpackaging into wet bottle devices of dry and wet mother bottles, wherein each bottle contains 20mL of the mixed solution to obtain the solution of the essence spray.

The preparation of the freeze-dried powder comprises the following steps:

(1) Dissolving 0.4g of trehalose in 1mL of ultrapure water to obtain a phase a;

(2) Filtering 0.2mL of SOD superoxide dismutase solution and 2mL of recombinant collagen solution with 0.22 μm microporous membrane respectively for sterilization, adding into phase a, and mixing;

(3) Placing the obtained mixed solution in a dry bottle device of a dry and wet primary bottle and fixedly placing the mixed solution in a vacuum freeze dryer, and freeze-drying to obtain freeze-dried powder;

(4) And (5) assembling the dry and wet primary and secondary bottles to obtain the essence spray for promoting hair growth and repairing hair roots.

Example 2

the preparation method is the same as example 1.

Example 3

The composition of the essential spray for promoting hair growth and repairing hair roots of this example was as follows (% represents mass percent):

the preparation method is the same as example 1.

Comparative example 1

The essence spray for promoting hair growth and repairing hair roots was prepared in the following manner: the method comprises the following steps of adding 5g of glycerol, 3g of 1, 3-butanediol, 1g of 1, 2-hexanediol, 3g of a swertia japonica extract, 0.5g of a green tea extract, 0.3g of rosemary leaf oil, 0.1g of mint essential oil, 0.01g of SOD superoxide dismutase, 0.1g of recombinant collagen and 2g of trehalose into the mixture, and finally adding ultrapure water to the final volume of 100mL. Filtering the obtained solution for sterilization, and subpackaging in spray bottles with 20 mL/bottle to obtain the final product.

Comparative example 2

The difference is that only SOD superoxide dismutase is used as a single component in the freeze-dried powder as in example 1.

Comparative example 3

The difference is that only a single component of recombinant collagen is present in the lyophilized powder as in example 1.

Comparative example 4

The difference is that only the vehicle had no lyophilised components as in example 1.

Comparative example 5

The difference from example 1 is that the recombinant collagen was replaced with equal mass of hydrolyzed keratin in the lyophilized powder.

Comparative example 6

The difference from example 1 is that the aqueous solution of swertia japonica Makino is not added.

Comparative example 7

The difference from example 1 is that the green tea extract was not added to the vehicle.

Investigation example 1 stability test

Electrophoresis analysis is carried out on the essence spray obtained in the comparative example 1 and the essence spray obtained in the example 1 in 12 months according to the compound essence spray obtained in the application example 1 in 12 months, the stability result is shown in figures 1 and 2, and the stability of the essence spray obtained in the example 1 is obviously superior to that of the comparative example 1.

Examination example 2 evaluation of safety

(1) Skin irritation test

The skin irritation test and evaluation were performed as specified in 2015 edition "technical standards for cosmetic safety", specifically: 4 white rabbits are taken, and 24 hours before the test, the hairs on two sides of the spine of the experimental animal back are cut off, the epidermis cannot be injured, and the hair removing range is about 3cm multiplied by 3cm on the left and the right respectively. About 0.5mL of the essence spray prepared in example 1 was applied directly to the skin, covered with two layers of gauze (2.5 cm. Times.2.5 cm), and fixed with a non-irritating adhesive tape and bandage. The other skin served as control. The application time is 2h by adopting a sealing test. After the test is finished, the residual test substance is removed by warm water or a non-irritant solvent. Skin reactions at the smeared sites were observed at 1, 24, 48 and 72h after removal of the test substances, skin reaction scores were made according to table 1, comprehensive evaluations were made with the mean value of the scores of the test animals, and skin irritation intensity was judged according to table 2 based on the highest mean value of the scores at each observation time point of 24, 48 and 72 h.

TABLE 1 skin irritation response Scale Table

Table 2 skin irritation intensity grading

TABLE 3 skin irritation response Scoring results for products

According to the skin irritation response scoring results of the product in table 3, the product is judged to be non-irritating to the skin.

(2) Skin allergy test

The Test and evaluation were carried out according to the skin allergy Test-topical closed skin Test (BT) specified in the cosmetic safety specification on the 2015 edition, in particular: 15 healthy guinea pigs were divided into 2 groups, a control group (5), and an experimental group (10). About 24 hours before the test, the left side of the back of the guinea pig is unhaired and removedThe wool range is 4cm ² -6cm ² . And in the induction contact stage, about 0.2ml of the test object is coated on the skin of a hair removal area of the experimental animal, covered by two layers of gauze and a layer of cellophane, and sealed and fixed by a non-irritant adhesive tape for 6 hours. The same procedure is repeated for 7d and 14 d. During the challenge period, 14-28 days after the last induction, approximately 0.2ml of the test substance was applied to the right 2cm x 2cm depilated area of the guinea pig back (24 h before challenge), covered with two layers of gauze and a layer of cellophane, and fixed with a non-irritating adhesive plaster for 6h. Skin reactions were observed 24h and 48h after challenge contact and scored according to table 4. The experiment was performed with sterile water as a negative control, water alone as a control at the induction contact, and the test substance at the challenge contact.

Table 4 allergy test skin reaction score

TABLE 5 allergy test skin response score

According to the scoring result of the skin allergy test-local closed coating test of the product in the table 5, the product is judged to have no skin allergy.

Examination example 3 rat Hair regeneration experiment

54 SD rats (200 g +/-20 g) with male and female halves; the hair is removed in the back area of the rat by shaving, and the skin of the shaved area is uniformly coated with depilatory cream (Veet) with the area of 3 x 3cm, 1 time every two days and 10 days later, a depilatory area is formed at the administration position, namely the molding is successful. 54 rats which are successfully modeled are selected and divided into 9 groups, and each group is half a female and half a male. 8 groups of rats were administered the essence sprays of examples 1 to 3 and comparative examples 2 to 7, respectively, and group 9 was administered physiological saline treatment as a blank control group; the administration mode is that essence spray is sprayed on the formed depilation region, 1mL is sprayed each time, and the spraying is carried out once a day; the administration was continued for 30 days. The amount of new hair is observed and recorded, and the evaluation standard is shown in a table 6; the length of 10 hairs of each group of animals was measured by pulling out the hair from the administration area, and the average length of the hair of each group of rats was calculated, and the calculation results are shown in Table 7.

TABLE 6 evaluation criteria for Hair amount

TABLE 7 Hair Length and Hair volume for different test groups

The essence spray prepared by the embodiment of the invention can effectively promote the growth of the hair of the rat and nourish the hair root. Compared with the comparative examples 2 to 7, the SOD superoxide dismutase compounded recombinant collagen can better promote the hair growth, stabilize hair roots and achieve better hair growth and hair care effects by combining the swertia japonica extract and the green tea extract.

Examination example 4 Effect of essence spray on SOD and MDA contents and Col I and Col III expressions in rat tissues

SOD and MDA contents and Col I and Col III expression levels in rat skin tissues in the investigation example 3 were analyzed. Compared with the detection results of comparative examples 2 to 4 (see table 8), the components of the essence spray prepared by the embodiment of the invention are synergistic, so that the expression of type I collagen and type III collagen in rat skin tissues can be effectively promoted, the proliferation and differentiation of hair follicle stem cells are stimulated, and damaged cells are rapidly repaired; SOD activity in tissues is obviously improved, MDA is obviously reduced, free radicals in rats are effectively removed, skin oxidative damage is protected, the activity of damaged cells is recovered, and then skin blood microcirculation is improved, and hair follicle development is promoted. The result shows that the SOD superoxide dismutase compounded recombinant collagen combined with the swertia japonica extract and the green tea extract can better repair and nourish hair roots and promote hair growth, and the hair growth and hair care effects of synergistic effect of 1+1 & gt 2 are achieved.

TABLE 8 Effect of the serum on SOD, MDA content and Col I, col III expression in rat skin tissue

P < 0.05, P < 0.01 compared to the blank control.

Investigation example 5 human body efficacy test

The essence sprays prepared in example 1 and comparative examples 2 to 4 were individually distributed to 50 test persons for testing. The use method of the essence spray is operated once a day by a tester, the tester scores the use effect of the essence spray according to the tester, each score is 10, and the higher the score is, the better the effect is. The results of the usage effect scoring are shown in table 9.

TABLE 9 evaluation chart for the effect of spraying essence

As can be seen from the data in table 9, the efficacy of the essence spray of example 1 was highly evaluated by the testers, and the total score was 25 minutes or more, and the results show that the components of the essence spray for promoting hair growth and repairing hair roots prepared by the present invention are synergistic, effectively remove in vivo toxins and free radicals, repair damaged hair follicle cells, promote hair growth, repair and nourish hair roots, and maintain healthy scalp microecology.

Claims

1. The essence spray for promoting hair growth and repairing hair roots is characterized by comprising a solvent and freeze-dried powder:

the menstruum comprises the following components in percentage by mass: 3 to 10 percent of glycerin, 0.1 to 10 percent of 1, 3-butanediol, 0.5 to 5 percent of 1, 2-hexanediol, 2 to 4 percent of swertia japonica extract, 0.1 to 2 percent of green tea extract, 0.3 to 0.5 percent of rosemary leaf oil, 0.1 to 0.3 percent of mint essential oil and the balance of ultrapure water;

the freeze-dried powder comprises the following components in percentage by mass and volume with a solvent: SOD superoxide dismutase (SOD) at a concentration of 1mg to 5mg/20mL, recombinant collagen at a concentration of 10mg to 50mg/20mL, and trehalose at a concentration of 0.2g to 1g/20mL.

2. The essence spray for promoting hair growth and repairing hair roots according to claim 1, wherein the molecular weight of the SOD superoxide dismutase is 10-30kDa, and the specific activity of the SOD superoxide dismutase is 10000-30000U/mg.

3. The essence spray for promoting hair growth and repairing hair roots according to claim 1, wherein the molecular weight of the recombinant collagen is 25kDa to 90kDa.

4. The essence spray for treating alopecia and repairing hair roots of claim 1, wherein the SOD superoxide dismutase and the recombinant collagen are obtained by gene recombination technology and microbial fermentation.

5. The essence spray for promoting hair growth and repairing hair roots according to claim 1, which is composed of a solvent and a lyophilized powder:

the menstruum comprises the following components in percentage by mass: 5% of glycerin, 3% of 1, 3-butanediol, 1% of 1, 2-hexanediol, 3% of a Japanese swertia herb extract, 0.5% of a green tea extract, 0.3% of rosemary leaf oil, 0.1% of mint essential oil and the balance of ultrapure water;

the freeze-dried powder comprises the following components in percentage by mass and volume with a solvent: SOD superoxide dismutase (SOD) at the concentration of 2mg-4mg/20 mL, recombinant collagen at the concentration of 10mg-20mg/20 mL and trehalose at the concentration of 0.4g/20mL.

6. The preparation method of the essence spray for treating alopecia and repairing hair roots according to any one of claims 1 to 5, which specifically comprises the steps of preparing a solvent and preparing a freeze-dried powder.

7. A method for preparing the essence spray for promoting hair growth and repairing hair roots according to claim 6, which comprises:

(1) Adding swertia japonica Makino extract, green tea extract, rosewood leaf oil, mint essential oil, glycerol, 1, 2-hexanediol and 1, 3-butanediol into ultrapure water, supplementing the balance of ultrapure water and stirring uniformly;

(2) Filtering the mixed solution with a 0.45-micron filter membrane, and subpackaging to obtain the solution of the essence spray.

8. The method for preparing essence spray for promoting hair growth and repairing hair roots according to claim 6, wherein the method for preparing the freeze-dried powder comprises the following steps:

dissolving trehalose in ultrapure water to obtain a phase a;

filtering SOD superoxide dismutase stock solution and recombinant collagen stock solution with 0.22 μm microporous membrane respectively for sterilization, adding into phase a, and mixing;

and S3, placing the obtained mixed solution in a container, and freeze-drying to obtain freeze-dried powder.

9. The method for preparing an essence spray for promoting hair growth and repairing hair roots according to claim 6, further comprising the step of dispensing and combining the solvent and the lyophilized powder in dry and wet mother-son bottles.

10. A method of using the essence spray for promoting hair growth and repairing hair roots prepared in claim 9, wherein the pump head is pressed down hard to pump the solvent into the upper layer of the lyophilized powder, and the lyophilized powder is shaken to dissolve sufficiently; after mixing, the essence is sprayed directly to the hair root of the head, and the part with hair skin exposed is sprayed more, and the hair is massaged by hand to complete absorption once a day.