CN113208966A

CN113208966A - Hair loss prevention and hair growth scalp care essence containing hinokitiol and preparation method and application thereof

Info

Publication number: CN113208966A
Application number: CN202110544627.3A
Authority: CN
Inventors: 胡根华; 吴知情; 胡根润; 万岳鹏
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2021-05-19
Filing date: 2021-05-19
Publication date: 2021-08-06

Abstract

The invention relates to an anti-hair-loss and hair-nourishing scalp care essence containing hinokitiol and a preparation method and application thereof, wherein the anti-hair-loss and hair-nourishing scalp care essence comprises the following raw materials: hinokitiol, swertia japonica extract, cacumen Platycladi extract, Polygoni Multiflori radix extract, Ginseng radix extract, and polyglycerol-10 laurate, while not containing ethanol. The hinokitiol mainly improves autophagy activity and mitochondrion autophagy activity of human hair papilla cells, the swertia japonica extract mainly expands peripheral blood vessels and promotes scalp blood circulation, the cacumen biotae extract, the polygonum multiflorum extract and the ginseng extract mainly improve activity of hair follicle cells, and the five components jointly promote hair growth in a multi-dimensional manner from different mechanism angles, so that the anti-hair loss and hair-growing effects have unexpected synergistic effects. Meanwhile, polyglycerol-10 laurate is used as a solvent for dissolving hinokitiol, and the formula is mild and has no stimulation.

Description

Hair loss prevention and hair growth scalp care essence containing hinokitiol and preparation method and application thereof

Technical Field

The invention belongs to the technical field of scalp care products, relates to an anti-hair loss and hair-growing scalp care essence and a preparation method and application thereof, and particularly relates to an anti-hair loss and hair-growing scalp care essence containing hinokitiol and a preparation method and application thereof.

Background

In recent years, more and more people suffer from alopecia, attention is paid to anti-alopecia and hair-growing technologies, and hair growth mainly depends on repeated division of hair mother cells at hair roots and supply nutrients to the capillaries under the scalp. While the papilla cells at the bottom of the hair follicle control the growth cycle of the hair by producing and releasing proliferative factors (anagen → catagen → telogen). Therefore, the technology of hair follicle cell activation and blood circulation promotion is an important link for preventing hair loss, growing and developing hair, and related researches show that the autophagy activity of the hair follicle in the catagen is lower than that in the anagen, when the expression of autophagy is inhibited, the hair follicle can be rapidly degenerated from the anagen to the catagen, and the activation of the autophagy activity can activate resting hair follicles and activate a new hair growth cycle.

CN110123659A discloses an anti-hair loss and hair-growing composition and application thereof in preparing an anti-hair loss and hair-growing washing and protecting product, the composition comprises inosine, lupin hydrolyzed protein and caffeine, the inosine, the lupin hydrolyzed protein and the caffeine are used in a matching manner, the anti-hair loss effect is remarkable, meanwhile, the composition also has the effect of promoting hair growth, the principle is that hair follicle cells on the scalp are stimulated, the hair follicle cells entering the dormant period grow again, the number of hair follicles in the growth period is increased, and the hair growth effect is achieved.

CN112022788A discloses an anti-hair loss and hair growth plant extract, the preparation raw materials of the plant extract are selected from at least two of ginger, red clover, swertia, cacumen biotae, folium artemisiae argyi, polygonum cuspidatum and astragalus, the plant composition has a synergistic interaction effect, and the action mechanism mainly comprises the functions of enhancing autophagy activity of hair papilla cells and promoting the proliferation of the hair papilla cells. In addition, the plant composition provided by the invention also has an obvious oil control effect, and achieves the effects of preventing hair loss and growing hair and refreshing and comfortable use feeling by inhibiting scalp oil secretion.

Most of products disclosed in the prior art only obtain the hair loss prevention and hair growth promotion effect from the consideration of a single target point, so the effect is limited, and if the hair loss prevention and hair growth promotion products can be started from a multi-path and multi-target angle, the development of the hair loss prevention and hair growth promotion products with remarkable hair loss prevention and hair growth promotion effects, mildness, no stimulation and stable system is very meaningful.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide an anti-hair loss and hair-growing scalp care essence and a preparation method and application thereof, and particularly provides an anti-hair loss and hair-growing scalp care essence containing hinokitiol and a preparation method and application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides an anti-hair loss and hair-growing scalp care essence containing hinokitiol, which is prepared from the following raw materials: hinokitiol, swertia japonica extract, cacumen Platycladi extract, Polygoni Multiflori radix extract, Ginseng radix extract, and polyglycerol-10 laurate, while not containing ethanol.

The anti-hair loss and hair-growing scalp care essence comprises five specific functional components, namely hinokitiol, a Japanese swertia herb extract, a cacumen biotae extract, a polygonum multiflorum extract and a ginseng extract, wherein the hinokitiol mainly improves autophagy activity and mitochondrion autophagy activity of human hair papilla cells, the Japanese swertia herb extract mainly expands peripheral blood vessels, promotes scalp blood circulation and improves skin temperature, the Chinese arborvitae herb extract, the polygonum multiflorum extract and the ginseng extract mainly improve hair follicle cell activity, and the five components start from different mechanism angles, promote hair growth in a multi-dimensional mode, and have unexpected synergistic effect on the anti-hair loss and hair-growing effects. Meanwhile, the polyglycerol-10 laurate is used as a solvent for dissolving hinokitiol, ethanol is not contained, the formula is mild and non-irritant, and the anti-hair loss and hair-growing scalp care essence liquid is stable and has no precipitation and suspension phenomena.

Preferably, the preparation raw materials of the anti-hair loss and hair-growing scalp care essence comprise, by taking the total mass of the preparation raw materials as 100%: 0.01-1% of hinokitiol, 1-5% of swertia japonica extract, 1-5% of biota orientalis extract, 1-5% of polygonum multiflorum extract, 1-5% of ginseng extract and 0.1-2% of polyglycerol-10 laurate.

When the hinokitiol, the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract and the ginseng extract are combined according to the specific mass ratio, the anti-hair-loss and hair-growing scalp care essence jointly promotes the growth of hair, and has a more remarkable synergistic effect on the anti-hair-loss and hair-growing effects.

The mass percentage of the hinokitiol can be 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

The content of the swertia japonica extract in percentage by mass can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% and the like.

The mass percentage of the cacumen biotae extract can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% and the like.

The mass percentage of the polygonum multiflorum extract can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% and the like.

The ginseng extract can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% and the like in percentage by mass.

The polyglycerol-10 laurate may be contained in an amount of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2% by mass or the like.

Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.

Preferably, the raw materials for preparing the anti-hair-loss and hair-nourishing scalp care essence further comprise any one or a combination of at least two of deionized water, a humectant, a preservative, a chelating agent or an essence.

The combination of at least two of the above-mentioned components, such as the combination of deionized water and humectant, the combination of humectant and preservative, the combination of preservative and chelating agent, etc., can be selected in any combination manner, and will not be described herein again.

The anti-hair loss and hair-growing scalp care essence can be selectively added with the auxiliary agent according to the functional requirements in actual application. The following specific types are preferred to make the product system stable, mild and non-irritating.

Preferably, the humectant comprises 1, 3-butanediol.

Preferably, the preservative comprises sodium benzoate and/or phenoxyethanol.

Preferably, the chelating agent comprises disodium EDTA.

In a second aspect, the present invention provides a method for preparing an anti-hair loss and hair-growing scalp care essence containing hinokitiol according to the first aspect, wherein the preparation method comprises the following steps:

(1) mixing and stirring part of deionized water, a water-soluble preservative and a chelating agent, and heating to obtain a material body A; mixing hinokitiol with a part of polyglycerol-10 laurate, stirring, heating, and adding a part of humectant to obtain a material body B;

(2) mixing and stirring the material body B and the material body A, and cooling to obtain a material body C;

(3) mixing and stirring the fat-soluble preservative, the rest of the humectant, the rest of the polyglycerol-10 laurate and the rest of the deionized water to obtain a material body D;

(4) and mixing and stirring the material D and the material C, and then mixing and stirring with the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract and the ginseng extract to obtain the anti-hair loss and hair-growing scalp care essence containing hinokitiol.

The preparation process of the anti-hair loss and hair-growing scalp care essence containing hinokitiol, which is disclosed by the invention, is relatively simple and easy to operate, is very suitable for large-scale industrial production, and has remarkable practicability.

Preferably, the portion refers to 10-90% of the total amount, such as 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, etc., and other specific points in the numerical range can be selected, which is not described herein again.

Preferably, the heating in step (1) refers to heating to 60-80 ℃, for example, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃ and the like, and other specific values in the numerical range can be selected, and are not described in detail herein.

Preferably, the temperature reduction in the step (2) is to reduce the temperature to 10-40 ℃, for example, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ and the like, and other specific values in the value range can be selected, and are not described in detail herein.

Preferably, the step (3) further comprises mixing and stirring essence.

Preferably, the mixing and stirring in step (3) is performed at 15-35 ℃, for example, 15 ℃, 20 ℃, 25 ℃, 30 ℃, 35 ℃ and the like, and other specific values in the numerical range can be selected, which is not described in detail herein.

When the preparation method is carried out according to the specific parameter value range, the system of the final product is more stable, and the anti-hair loss and hair growing effects are promoted to be exerted.

In a third aspect, the invention provides a use of the anti-hair loss and hair-growing scalp care essence containing hinokitiol according to the first aspect in preparing an anti-hair loss and hair-growing product.

The anti-hair loss and hair-nourishing scalp care essence can be further added into the following anti-hair loss and hair-nourishing products for application.

Preferably, the anti-hair loss and hair-nourishing product comprises shampoo, shampoo cream and hair conditioner.

Compared with the prior art, the invention has the following beneficial effects:

the anti-hair loss and hair-growing scalp care essence comprises five specific functional components, namely hinokitiol, a Japanese swertia herb extract, a cacumen biotae extract, a polygonum multiflorum extract and a ginseng extract, wherein the hinokitiol mainly improves autophagy activity and mitochondrion autophagy activity of human hair papilla cells, the Japanese swertia herb extract mainly expands peripheral blood vessels, promotes scalp blood circulation and improves skin temperature, the Chinese arborvitae herb extract, the polygonum multiflorum extract and the ginseng extract mainly improve hair follicle cell activity, and the five components start from different mechanism angles, promote hair growth in a multi-dimensional mode, and have unexpected synergistic effect on the anti-hair loss and hair-growing effects. Meanwhile, the polyglycerol-10 laurate is used as a solvent for dissolving hinokitiol, ethanol is not contained, the formula is mild and non-irritant, and the anti-hair loss and hair-growing scalp care essence liquid is stable and has no precipitation and suspension phenomena. The preparation process is relatively simple and easy to operate, is very suitable for large-scale industrial production, and has remarkable practicability. It can be further added to the following hair loss prevention and hair growth promotion products for application.

Drawings

FIG. 1 is a statistical chart showing the results of measurement of autophagy activity of human dermal papilla cells in test example 1.

FIG. 2 is a graph showing the results of measurement of the mitophagy activity of human dermal papilla cells in test example 2.

Fig. 3 is an observation graph of human blood flow in test example 3.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The following examples and comparative examples refer to the following sources of raw materials:

hinokitiol, purchased from high sand perfumery, under the brand name hinokitiol;

SWERTIA japonica EXTRACT, purchased from suyota sacchari corporation under the designation SWERTIA HERB EXTRACT B;

ginseng radix extract, purchased from Shanghai JiaKai Biotech limited, under the brand name of Ginseng radix extract;

radix Polygoni Multiflori extract, purchased from Shanghai Jia Kai Biotech Co., Ltd, having a trade mark of radix Polygoni Multiflori extract;

cacumen Platycladi extract, available from Shanghai Jiaqiao Biotech Co., Ltd, under the brand name cacumen Platycladi extract;

polyglycerol-10 laurate, purchased from Sun chemical, under the designation SUNSOFT M-12J;

essence, purchased from Changgang industries, Inc. under the designation 132.159-A;

human hair papilla cells, purchased from toyo kayaku under the designation CA60205 a;

Cyto-ID Green Detection Reagent 2, purchased from Enzo Life Sciences, Inc., under the designation ENZ-51031-K200;

Cyto-ID Autophary Detection Kit, purchased from Enzo Life Sciences, Inc., under the brand name ENZ-KIT 175-0050;

the Mitophagy Dye Detection kit is purchased from the institute of homonymy chemical research, Inc. and is under the trademark Mitophagy Dye Detection kit;

microplate Reader POWERSCAN HT, available from DS PHARMA BIOMEDICAL co, ltd, under the designation POWERSCAN HT;

a cell imager, purchased from Life Technologies Japan Ltd, under the trademark Tali Image Cytometer;

blood flow meter, purchased from de corporation under the designation TOKU Capillaro.

Other reagents and instruments are commercially available.

Example 1

The embodiment provides an anti-hair loss and hair-growing scalp care essence which comprises the following raw materials in percentage by mass: 0.5% of hinokitiol, 2% of swertia japonica Makino extract, 1.5% of cacumen biotae extract, 1.2% of polygonum multiflorum extract, 1% of ginseng extract, 1.5% of polyglycerol-10 laurate, 3% of 1, 3-butanediol, 0.25% of sodium benzoate, 0.2% of phenoxyethanol, 0.1% of EDTA disodium, 0.3% of essence and the balance of deionized water.

The preparation method comprises the following steps:

(1) mixing and stirring half of the added amount of deionized water, sodium benzoate and EDTA disodium, and heating to 70 ℃ to obtain a material body A; mixing hinokitiol with half of the addition amount of polyglycerol-10 laurate, stirring, heating to 70 ℃, and then adding half of the addition amount of 1, 3-butanediol to obtain a material body B;

(2) mixing and stirring the material body B and the material body A, and cooling to 30 ℃ to obtain a material body C;

(3) mixing and stirring phenoxyethanol, the rest of 1, 3-butanediol, the rest of polyglycerol-10 laurate and the rest of deionized water to obtain a material body D;

(4) and mixing and stirring the material D and the material C, and then mixing and stirring the mixture with the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract, the ginseng extract and the essence at 25 ℃ to obtain the anti-hair loss and hair-growing scalp care essence containing hinokitiol.

Example 2

The embodiment provides an anti-hair loss and hair-growing scalp care essence which comprises the following raw materials in percentage by mass: 0.05% of hinokitiol, 1% of swertia japonica extract, 1% of cacumen biotae extract, 5% of polygonum multiflorum extract, 2% of ginseng extract, 0.5% of polyglycerol-10 laurate, 2% of 1, 3-butanediol, 0.25% of sodium benzoate, 0.2% of phenoxyethanol, 0.1% of EDTA disodium, 0.3% of essence and the balance of deionized water.

The preparation method comprises the following steps:

(1) mixing and stirring half of the added amount of deionized water, sodium benzoate and EDTA disodium, and heating to 60 ℃ to obtain a material body A; mixing hinokitiol with half of the addition amount of polyglycerol-10 laurate, stirring, heating to 60 ℃, and then adding half of the addition amount of 1, 3-butanediol to obtain a material body B;

(2) mixing and stirring the material body B and the material body A, and cooling to 20 ℃ to obtain a material body C;

(4) and mixing and stirring the material D and the material C, and then mixing and stirring the mixture with the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract, the ginseng extract and the essence at 20 ℃ to obtain the anti-hair loss and hair-growing scalp care essence containing hinokitiol.

Example 3

The embodiment provides an anti-hair loss and hair-growing scalp care essence which comprises the following raw materials in percentage by mass: 0.8% of hinokitiol, 3% of swertia japonica extract, 3% of cacumen biotae extract, 4% of polygonum multiflorum extract, 1% of ginseng extract, 1.6% of polyglycerol-10 laurate, 2.5% of 1, 3-butanediol, 0.25% of sodium benzoate, 0.2% of phenoxyethanol, 0.1% of EDTA disodium, 0.3% of essence and the balance of deionized water.

The preparation method comprises the following steps:

(1) mixing and stirring half of the added amount of deionized water, sodium benzoate and EDTA disodium, and heating to 80 ℃ to obtain a material body A; mixing hinokitiol with half of the addition amount of polyglycerol-10 laurate, stirring, heating to 80 ℃, and then adding half of the addition amount of 1, 3-butanediol to obtain a material body B;

(2) mixing and stirring the material body B and the material body A, and cooling to 40 ℃ to obtain a material body C;

(4) and mixing and stirring the material D and the material C, and then mixing and stirring the mixture with the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract, the ginseng extract and the essence at 35 ℃ to obtain the anti-hair loss and hair-growing scalp care essence containing hinokitiol.

Comparative example 1

The present comparative example provides an anti-alopecia and hair-growing scalp care essence, which is different from example 1 only in that hinokitiol is not contained, and the reduced amount thereof is distributed to the mass of swertia japonica thunb extract, biota orientalis leaf extract, polygonum multiflorum thunb extract and ginseng extract according to the mass ratio, and other conditions are maintained. The preparation process is as in example 1.

Comparative example 2

The present comparative example provides an anti-alopecia and hair-growing scalp care essence, the formulation composition of which differs from that of example 1 only in that no japanese swertia herb extract is contained, and the reduced amount thereof is distributed to the mass of hinokitiol, cacumen biotae extract, polygonum multiflorum extract and ginseng extract according to the mass ratio, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 3

The present comparative example provides a scalp care essence for preventing hair loss and growing hair, which is different from example 1 only in that it does not contain cacumen biotae extract, and the reduced amount thereof is distributed to the mass of hinokitiol, swertia japonica extract, polygonum multiflorum extract and ginseng extract according to the mass ratio, and the other conditions are maintained.

The preparation process is as in example 1.

Comparative example 4

The present comparative example provides a scalp care essence for preventing hair loss and growing hair, which is different from example 1 only in that it does not contain the polygonum multiflorum extract, and the reduced amount thereof is distributed to the mass of hinokitiol, the swertia japonica extract, the biota orientalis extract, and the ginseng extract according to the mass ratio, and the other conditions are maintained.

The preparation process is as in example 1.

Comparative example 5

The present comparative example provides an anti-alopecia and hair-growing scalp care essence, which is different from example 1 only in that ginseng extract is not contained, and the reduced amount thereof is distributed to the mass of hinokitiol, swertia japonica extract, biota orientalis extract and polygonum multiflorum extract according to the mass ratio, and other conditions are kept unchanged.

The preparation process is as in example 1.

Comparative example 6

The comparison example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the scalp care essence is different from that of example 1 only in that the scalp care essence does not contain the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract and the ginseng extract, the mass percentage content of hinokitiol is 6.2%, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 7

The comparative example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the scalp care essence is different from that of example 1 only in that hinokitiol, a cacumen biotae extract, a polygonum multiflorum extract and a ginseng extract are not contained, the mass percentage content of the swertia japonica extract is 6.2%, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 8

The comparative example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the scalp care essence is different from that of example 1 only in that hinokitiol, a swertia japonica extract, a polygonum multiflorum extract and a ginseng extract are not contained, the mass percentage content of the cacumen biotae extract is 6.2%, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 9

The comparative example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the scalp care essence is different from that of example 1 only in that hinokitiol, a cacumen biotae extract, a swertia japonica extract and a ginseng extract are not contained, the mass percentage content of a polygonum multiflorum extract is 6.2%, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 10

The comparative example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the scalp care essence is different from that of example 1 only in that hinokitiol, a cacumen biotae extract, a polygonum multiflorum extract and a swertia japonica extract are not contained, the mass percentage content of the ginseng extract is 6.2%, and other conditions are kept unchanged. The preparation process is as in example 1.

Comparative example 11

The comparison example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the essence is different from that of example 1 only in that polyglycerol-10 laurate is not contained, the reduction amount is distributed to the quality of deionized water, and other conditions are kept unchanged. The preparation method comprises the following steps:

(1) mixing and stirring half of the added amount of deionized water, sodium benzoate and EDTA disodium, and heating to 70 ℃ to obtain a material body A; heating hinokitiol to 70 ℃, and then adding half of the added amount of 1, 3-butanediol to obtain a material body B;

Comparative example 12

The comparison example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the anti-hair loss and hair-growing scalp care essence is different from that of example 1 only in that the mass percentage content of polyglycerol-10 laurate is 0.05%, the reduction amount is distributed on the mass of deionized water, and other conditions are kept unchanged. Its preparation method reference is made to example 1.

Comparative example 13

The comparison example provides an anti-hair loss and hair-growing scalp care essence, and the formula composition of the anti-hair loss and hair-growing scalp care essence is different from that of example 1 only in that the mass percentage content of the polyglycerol-10 laurate is 0.09%, the reduction amount is distributed on the mass of the deionized water, and other conditions are kept unchanged. Its preparation method reference is made to example 1.

Test example 1:

determination of autophagy activity of human hair papilla cells:

human dermal papilla cells cultured in the dermal papilla cell growth medium were seeded in a 96-well plate in an amount of 10,000cells/well and cultured for 24 h. Then, human dermal papilla cells were cultured in a dermal papilla cell basal medium for 48 hours with and without a hinokitiol solution (3.125. mu.g/mL, solvent 1, 3-butanediol) with 10. mu. mol/L chloroquinone as a positive control. Fluorescent reagents were prepared by adding 10. mu.L of Hoechst 33342 nuclear stain and 10. mu.L of Cyto-ID Green Detection Reagent 2 to 1mL of 10 × assay buffer and 9mL of purified water, respectively. After washing with 1 × assay buffer, 100 μ L of fluorescent reagent was added to each well and incubated in an incubator for 30 min. The fluorescent reagent was recovered, washed twice with 200. mu.L of 1 × assay buffer, and 100. mu.L of 1 × assay buffer was added. Hoechst 33342 (excitation: 360/40, emission: 460/40) and Cyto-ID Green Detection Reagent 2 (excitation: 485/20, emission: 528/20) were measured with a fluorescence plate analyzer. Experiments were performed at n-3 and mean and standard deviation were calculated.

As a result, as shown in FIG. 1, hinokitiol at a concentration of 3.125. mu.g/mL significantly enhanced the fluorescence intensity of Cyto-ID corrected with H33342, i.e., hinokitiol significantly increased the autophagy activity of human dermal papilla cells.

Test example 2:

determination of mitochondrial autophagy activity of human hair papilla cells:

human dermal papilla cells cultured in a dermal papilla cell growth medium were seeded in an amount of 200,000cells/well onto 6-well plates and cultured for 24 h. Then, human dermal papilla cells were cultured in a dermal papilla cell basal medium for 48 hours with and without a hinokitiol solution (3.125. mu.g/mL, solvent 1, 3-butanediol) with 500nmol/L of Rapamicin as a positive control. Cells were harvested with trypsin EDTA solution and 10. mu.L of mitochondrial dye at 100. mu. mol/L was added to 10mL of serum-free medium to prepare fluorescent reagent 2. The collected cells were washed with serum-free medium, 1mL of fluorescent reagent 2 was added, respectively, and placed in an incubator for 30 min. Fluorescent reagent 2 was recovered, washed twice with 200. mu.L serum-free medium, and then 100. mu.L serum-free medium was added. The percentage of cells stained with mitochondrial autophagy dye was measured with a Image Cytometer Tali Image Cytometer.

As shown in FIG. 2, hinokitiol at a concentration of 3.125. mu.g/mL can significantly increase the mitophagy dye-positive cell rate, i.e., hinokitiol can significantly increase the mitophagy activity.

Test example 3:

testing the blood flow volume of a human body:

after applying the oil to the ring finger of a non-dominant hand (generally, the default is left hand), video of blood flow was recorded by using a blood flow meter, and then 0.2g of a solution of swertia japonica extract (1, 3-butanediol having an active content of 1.5% and a solvent of 49.25%) was applied to the back of the hand of the same hand, and video was recorded after the sample was absorbed. And judging whether the effect of promoting blood circulation is effective or not according to the video animation result.

As a result, as shown in the static chart of FIG. 3, the capillaries smeared with the swertia japonica Makino extract liquid became more clearly visible due to the increase in the blood flow volume and the increase in the blood flow rate. In addition, the video of the test results also showed an increase in the blood flow rate of the capillaries after application of the swertia japonica extract.

Test example 4:

and (3) testing the system stability:

because the hinokitiol in the anti-hair loss and hair-growing scalp care essence is slightly soluble in water and is difficult to apply to an alcohol-free formula, the hinokitiol is solubilized into the formula creatively through the mild and non-irritant polyglycerol-10 laurate.

The anti-alopecia and hair-growing scalp care essences prepared in examples 1 to 3 and comparative examples 1 to 13 were left at 25 ℃, 40 ℃, 50 ℃ and 5 ℃ for 30 days, and the appearance of the samples was visually observed, and the test results are shown in table 1 (o indicates that the state is intact, the uniform and stable liquid state is maintained, and x indicates that delamination and precipitation occur under any of the test conditions).

TABLE 1

As can be seen from the data in Table 1, the anti-hair-loss and hair-growing scalp care essence disclosed by the invention solubilizes hinokitiol into a formula through the mild and non-irritant polyglycerol-10 laurate, so that the stability is good, and the shampoo is mild and non-irritant.

Test example 5:

anti-alopecia hair-growing clinical test:

130 volunteers (39 30-40 years old, 39 40-50 years old and 52 50-60 years old) were selected and randomly divided into 13 groups, 10 persons (3 30-40 years old, 3 40-50 years old and 4 persons 50-60 years old) were selected, and the anti-hair loss and hair-growing scalp care essence liquids prepared in examples 1-3 and comparative examples 1-10 were respectively tried on each group.

Two selected areas of the same area (2cm x 2cm) of the subject were shaved and the bare scalp was applied with the test sample on one side and without any hair loss prevention hair growth product treatment on the other side as a blank control. The scalp condition before and after shaving was photographed by using a microscope for 30 days after applying the hair cream 1 time per day, 200. mu.L each time, continuously for 30 days. The average diameter, average density and average length of the grown hair were observed and counted by image analysis. And the subject is allowed to carry out subjective evaluation and scoring (comprehensive use feeling, actual feeling of the hair loss prevention and hair growth effect and mild feeling) on the product, the scoring range is 1-5 points, then the average value is taken, the score is 1-2 is poor, the score is 2-3 is general, the score is 3-4 is good, and the score is 4-5 is good. The results are shown in table 2:

TABLE 2

Group of

Diameter (μm)

Density (root/cm)²)

Length (cm)

Feeling of use

Effect feeling

Mildness property

Example 1

85.6

62.5

1.15

Good effect

Is preferably used

Has no irritation

Example 2

84.1

62.0

1.14

Good effect

Is preferably used

Has no irritation

Example 3

86.4

65.7

1.20

Good effect

Has no irritation

Comparative example 1

70.2

55.0

1.05

Good effect

In general

Has no irritation

Comparative example 2

71.0

56.4

0.95

Good effect

In general

Has no irritation

Comparative example 3

70.6

55.8

0.95

Good effect

In general

Has no irritation

Comparative example 4

74.5

57.2

1.00

Good effect

In general

Has no irritation

Comparative example 5

74.9

57.5

1.00

Good effect

In general

Has no irritation

Comparative example 6

65.4

35.0

0.88

In general

Is poor

Has no irritation

Comparative example 7

62.8

36.2

0.85

In general

Is poor

Has no irritation

Comparative example 8

60.4

34.1

0.84

In general

Is poor

Has no irritation

Comparative example 9

61.5

33.8

0.80

In general

Is poor

Has no irritation

Comparative example 10

60.8

32.7

0.81

In general

Is poor

Has no irritation

From the results of table 2, it can be seen that: compared with the comparative example, the anti-hair-loss and hair-nourishing scalp care essence has unexpected synergistic effect on the anti-hair-loss and hair-nourishing effect of five specific functional components, namely hinokitiol, the swertia japonica extract, the biota orientalis extract, the polygonum multiflorum extract and the ginseng extract. Meanwhile, the user feels good in comprehensive use, actual feeling of the hair loss prevention and hair growth effect and mildness.

The applicant states that the invention is described by the above embodiments to describe the hair loss prevention and hair growth promotion scalp care essence, the preparation method and the application thereof, but the invention is not limited to the above embodiments, that is, the invention is not limited to the above embodiments. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

Claims

1. The anti-hair-loss and hair-growing scalp care essence containing hinokitiol is characterized by comprising the following preparation raw materials: hinokitiol, swertia japonica extract, cacumen Platycladi extract, Polygoni Multiflori radix extract, Ginseng radix extract, and polyglycerol-10 laurate, while not containing ethanol.

2. The anti-hair-loss scalp care essence containing hinokitiol according to claim 1, wherein the anti-hair-loss scalp care essence is prepared from the following raw materials by 100% of the total mass of the raw materials: 0.01-1% of hinokitiol, 1-5% of swertia japonica extract, 1-5% of biota orientalis extract, 1-5% of polygonum multiflorum extract, 1-5% of ginseng extract and 0.1-2% of polyglycerol-10 laurate.

3. The anti-hair-loss and hair-growing scalp care essence containing hinokitiol according to claim 1 or 2, wherein the anti-hair-loss and hair-growing scalp care essence is prepared from a raw material further comprising any one or a combination of at least two of deionized water, a humectant, a preservative, a chelating agent or an essence.

4. The anti-hair-loss scalp care essence containing hinokitiol according to claim 3, wherein the moisturizer comprises 1, 3-butanediol.

5. The anti-alopecia, hair-growing scalp care essence containing hinokitiol according to claim 3, wherein the preservative comprises sodium benzoate and/or phenoxyethanol.

6. The anti-hair-loss scalp care essence containing hinokitiol according to claim 3, wherein the chelating agent comprises disodium EDTA.

7. The method for preparing anti-hair loss and hair-growing scalp care essence containing hinokitiol according to any one of claims 1 to 6, comprising the steps of:

8. The method of claim 7, wherein the fraction is 10-90% of the total;

preferably, the heating in step (1) is heating to 60-80 ℃;

preferably, the temperature reduction in the step (2) is to reduce the temperature to 10-40 ℃.

9. The method according to claim 7 or 8, wherein the step (3) further comprises mixing and stirring the essence;

preferably, the mixing and stirring of step (3) is carried out at 15-35 ℃.

10. Use of hinokitiol-containing anti-hair loss scalp care essence according to any one of claims 1 to 6 for the preparation of an anti-hair loss hair-growing product;

preferably, the anti-hair loss and hair-nourishing product comprises shampoo, shampoo cream or hair conditioner.