CN102274359B - Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof - Google Patents

Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof Download PDF

Info

Publication number
CN102274359B
CN102274359B CN 201110197132 CN201110197132A CN102274359B CN 102274359 B CN102274359 B CN 102274359B CN 201110197132 CN201110197132 CN 201110197132 CN 201110197132 A CN201110197132 A CN 201110197132A CN 102274359 B CN102274359 B CN 102274359B
Authority
CN
China
Prior art keywords
vitiligo
parts
water
masking sol
covering liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN 201110197132
Other languages
Chinese (zh)
Other versions
CN102274359A (en
Inventor
雷红
王青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wang Qing
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN 201110197132 priority Critical patent/CN102274359B/en
Publication of CN102274359A publication Critical patent/CN102274359A/en
Application granted granted Critical
Publication of CN102274359B publication Critical patent/CN102274359B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention relates to a vitiligo covering liquid treating both the manifestation and root cause. The liquid is prepared from the following raw materials by weight: 50-100 parts of fig leaf, 50-100 parts of duckweed, 50-100 parts of orange peel, 200-300 parts of 75% ethanol, 10-20 parts of cupric chloride, and 700-800 parts of towel gourd water. The preparation method includes the steps of: soaking the fig leaf, duckweed and orange peel respectively in water, drying and cutting them into filaments, and then fully mixing the three kinds of filaments well; adding the 75% ethanol solution to soak for 48 hours, filtering the herb residues so as to obtain a pigment solution; adding the cupric chloride into the pigment solution and mixing them uniformly; then adding the towel gourd water and fully mixing well, thus obtaining the vitiligo covering liquid. The vitiligo covering liquid of the invention has both the efficacy of natural pigments in covering leukoderma and the function of natural plant extracts in activating melanocyte tyrosinase activity so as to promote melanin generation. Therefore, the vitiligo covering liquid provided in the invention can treat both the manifestation and root cause of the disease, and is safety in use and free of adverse reactions.

Description

Treating both the principal and secondary aspects of a disease type vitiligo Masking sol and preparation method
Technical field
The invention belongs to therapy of vitiligo make-up technique field, be specifically related to a kind of vitiligo Masking sol, have the function of the treating both the principal and secondary aspects of a disease that hides white macula and melanotropin generation, and announce its prescription and preparation method thereof.
Background technology
Vitiligo is a kind of common posteriority pigment disappearance dermatoses, and sickness rate is about 1%-2%, is one of world's dermatosis three big pertinacious diseases.Leucoderma disease is because of complexity, and its pathogeny is not clear and definite fully as yet at present.Directly the cause of disease is that the melanin of skin and hair follicle takes off to become homeless and causes, is easy to diagnosis clinically and is difficult to treatment.The vitiligo symptom shows as skin and limitation or general property white macula occur, the white macula form differs, can occur in any position of health, the general multiple position that skin exposes and is subject to rub of being born in is as facial area, cervical region, forearm, the back of the hand, shank, waist and belly etc.Though vitiligo does not influence patient's body health and physiological activity substantially, but because the skin lesion that primary disease causes directly influences patient's outward appearance appearance, bring great stress and psychological burden to them, all brought many negative effects at aspects such as life, study, work, social activities.
Though vitiligo is difficult to cure, Therapeutic Method still is many.The several different methods such as composite treatment, part and whole combined therapy, the combined therapy of Chinese and Western medicine, medicated physical treatment combined therapy that adopt are intended to improve therapeutic effect more at present, reduce side effect.But it is few to treat leukodermic good medicine at present, and curative effect is dissatisfied.In order to alleviate the misery of patients with vitiligo physiology and psychology, market demand is developed a kind of cosmetic medicine that hides cosmetic result that has, and to satisfy patient's Physiological Psychology demand, alleviates the patient suffering.Natural plant extracts effective ingredient and natural pigment have preferable effect aspect therapy of vitiligo and the covering, and therefore, develop this type of vitiligo covering product and have good prospect, be very useful and necessary to the patient.
Summary of the invention
The purpose of this invention is to provide a kind of leukoderma covering agent, it has the function that hides white macula and melanotropin generation, has treating both the principal and secondary aspects of a disease, uses safe characteristics.
Concrete technical solution is as follows:
(same claims)
Folium Fici in the present invention's prescription is the leaf of moraceae plants Fructus Fici, and main component is psoralen, bergapton, cupreol, β-Amyrin and Lupeol; Contain Palmic acid, valeric acid, guaiacol, 28 alkane, globulariacitrin again; Other contains the furoeoumarin class, and contact people's skin can be to photaesthesia; Have removing toxic substances and promoting subsidence of swelling, promoting the circulation of QI to relieve pain effect.Herba Spirodelae is the herb of plant and of its applications duckweed spirodela or blue or green duckweed, is common water surface floating plant in China; Main component is flavone compounds such as red grass element, vitexin, also contains materials such as carotene, phylloxanthin, potassium acetate, potassium chloride, iodine, bromine fatty acid.The Pericarpium Citri junoris ethanol soluble extraction has makes yellowish-brown pigment be deposited on the effect of skin.Copper ion in the copper chloride is the important meals ion that promotes multiple catalyzing enzyme activity in the melanin building-up process.Contain abundant vitamin such as vitamin A, vitamin E etc. in the Fructus Luffae water, also contain several amino acids and mineral element, as calcium, phosphorus, zinc, copper, ferrum etc., and contain catalase, superoxide dismutase, let out root acid etc., have and preserve moisture, anti-ageing wrinkle removal, antiinflammatory, prevent functions such as capillary hemorrhage; Multiple triterpene saponin is arranged in the Fructus Luffae water, and the energy softening skin is strengthened cell viability, stimulates epithelial division, accelerates the regeneration of Skin Cell; Copper in the Fructus Luffae water can be participated in the metabolic process of human body pigment directly, rising skin tyrosinase activity, and catalytic tyrosine forms melanin and causes cutaneous pigmentation.
The pharmacology pharmacodynamic experimental study shows that vitiligo Masking sol of the present invention has the effect that hides white macula, activation melanocyte tyrosinase activity, promotes melanin generation in the melanocyte; Local irritation experiment safety evaluation study shows safety of the present invention, untoward reaction such as no skin irritation, allergy.The patients with vitiligo result on trial shows that the present invention has the white macula of covering, melanotropin systematic function, uses safety to have no side effect.
Be the experiment of pharmacology pharmacodynamic of the present invention and local irritation safety evaluatio and data below:
Experiment one: adopt experimental vitiligo Cavia porcellus animal model.Get 30 of Cavia porcelluss, shave off back wool area 4cm X 4cm with shaver.Be divided into 3 groups at random: normal control group, model control group, vitiligo Masking sol group, 10 every group.Model control group, vitiligo Masking sol group are coated with 5% hydrogen peroxide 1ml in the depilation district, every day 2 times, continuous 30 days, set up experimental vitiligo animal model; The normal control group is coated with the equivalent purified water, every day 2 times, continuous 30 days in the depilation district.During this period, each organizes Cavia porcellus depilation in per 3 days once.After the modeling success, each Cavia porcellus of vitiligo Masking sol group is outer respectively to be coated with vitiligo Masking sol 1ml of the present invention, smears the back and observes 15 days.Experimental result shows that normal control group guinea pig skin hair color is constant; The model control group guinea pig skin is obviously pale, the appearance white macula that has, and the desquamation degree is more serious; Vitiligo Masking sol group Cavia porcellus 2-4 hour skin color after smearing the present invention begins to change, and skin was sepia in 8-24 hour, and near the normal control group, color can be kept 5-15 days.
Experiment two: tyrosinase activity is measured: after vitiligo Masking sol 0.5ml of the present invention is diluted with 1/15mol/L pH 6.8 phosphate buffer 0.5ml, 100 μ l (60U) mix with Mushroom Tyrosinase, hatched 10 minutes for 25 ℃, add 3mol/L L-dopa reaction liquid 2ml, continued to hatch 2 minutes, immediately in spectrophotometer 475nm place's photometry absorption value A value.Other establishes distilled water blank group, in spectrophotometer 475nm place's photometry absorption value A value.Two groups of biochemical reaction experiments all repeat 10 times.The tryrosinase activity ratio calculates by following formula: the empty X 100% of activity ratio (%)=(A medicine-A sky)/A.The result shows that the tryrosinase activity ratio is 78.33 ± 11.46%.Illustrate that vitiligo Masking sol of the present invention has the effect of activity of tyrosinase.
Experiment three: In vitro culture normal epidermis melanocyte, (second filial generation) melanocyte that will go down to posterity counting guarantees that by planting in 6 culture bottles every bottle of cell number is identical, other establishes 1 bottle and is the blank group.Add vitiligo Masking sol 100 μ l of the present invention in 6 culture bottles respectively, 200 μ l, 400 μ l, 600 μ l, 800 μ l, 1000 μ l cultivated 3 days.Peptic cell is again by planting in 96 orifice plates (every porocyte several 2 * 10 3).Adopt the dissolution of sodium hydroxide method to measure the cell melanin content, every hole adds the sodium hydroxide of 100 μ l 1mol/L, and 37 ℃ are incubated 1 hour, survey it at the absorbance at 450nm place, draw the melanocyte standard curve, calculate melanin content.The result shows (seeing Table 1), compares with the blank group, and the vitiligo Masking sol can make the interior melanin content of melanocyte obviously raise.Illustrate that the present invention has the effect that promotes that melanin generates.
Adopt tetramethyl azo azoles salt (MTT) colorimetric determination cell proliferation vigor, (every porocyte number is 2 * 10 in 96 orifice plates with cell inoculation 3), after still cultivating 3 days with above-mentioned 6 kinds of drug doses, the sucking-off culture fluid, add MTT solution 100 μ l in every hole in 37 ℃ of reactions 4 hours, discard MTT, every hole adds 100 μ l DMSO and vibrated 10 minutes, abundant dissolving crystallized thing, the 490nm place measures the absorbance A value, and experimental result is represented (experimental group A value/control group A value * 100) with cell proliferation rate.The result shows (seeing Table 2), compares with the blank group, and the vitiligo Masking sol can make the melanocyte rate of increase obviously raise.Illustrate that the present invention has the effect that promotes melanocytic hyperplasia.
Be substrate with L-Dopa, adopt external oxidation dopa reaction method to measure the melanocyte tyrosinase activity.(every porocyte number is 2 * 10 in 96 orifice plates with cell inoculation 3), after still cultivating 72 hours with above-mentioned 6 kinds of drug doses, collecting cell, centrifugal abandoning supernatant, PBS with 0.01mol/L washes 2 times, add 200 μ L, 0.1% TritonX-100 lysate, placed-80 ℃ of refrigerators 0.5 hour, rewarming is 0.5 hour in 37 ℃ of water baths, add 2mg/mL L-Dopa 400 μ L, continue 37 ℃ and hatched 2 hours, spectrophotometer reads 450nm place absorbance A value, proofreaies and correct with the autoxidation of L-Dopa.Repeat 3 times.Experimental result is represented with tyrosinase activity (%)=(medication group A value-blank group A value)/blank group A value * 100%.The result shows (seeing Table 3), compares with the blank group, and the vitiligo Masking sol has significant potentiation to the melanocyte tyrosinase activity.Tryrosinase is a kind of copper enzyme, is melanogenic key enzyme in the mammalian cell.Illustrate that the present invention can promote melanic generation in the melanocyte by activating the melanocyte tyrosinase activity.
The influence of melanin content in the table 1. pair melanocyte
Figure BSA00000537844800031
X ± s.n=12. compares with blank, *P＜0.05, *P＜0.01.
The influence of table 2. pair melanocyte propagation
Figure BSA00000537844800032
X ± s.n=12. compares with blank, *P＜0.01.
The influence of table 3. pair tyrosinase activity
Figure BSA00000537844800033
X ± s.n=12. compares with blank, *P＜0.01.
Experiment four: local irritation experiment
Get 8 of adult rabbit, male and female half and half, every animal sub-cage rearing was raised 3-4 days at laboratory condition earlier.Then rabbit spinal column both sides dorsal body setae is shaved off every lateral area 5 * 10cm with electric shaver-for women 2Whether scrutiny depilation district has redness and damage, if any abandoning it, can not test.Adopt the test method of multiple dosing, vitiligo Masking sol of the present invention is smeared in the district in depilation, every day 1 time, gives 7 days continuously.Whether last gives after 24 hours to observe the local skin reaction with warm water flush away baste after 1,24,48,72 hour, as erythema, edema, desquamation, incrustation etc., have petechia, skin peptide coarse or poor etc., record time of origin and regression time.The result shows, is smearing the present invention continuously after 7 days, and each rabbit skin does not all show tangible skin irritation and anaphylaxis.Show product safety of the present invention, have no adverse reaction.
The present invention is compared with prior art: the present invention is that raw material is made with Folium Fici, Herba Spirodelae and Pericarpium Citri junoris alcohol extract, copper chloride, Fructus Luffae water, existing natural pigment hides leukoderma performance beauty functions, natural plant extracts activation melanocyte tyrosinase activity is arranged again and promote the effect that melanin generates, so the present invention has the effect for the treatment of both the principal and secondary aspects of a disease to vitiligo; And use safety has no adverse reaction.
The specific embodiment
Below in conjunction with embodiment, the present invention is done to describe further.
Embodiment 1:
Get Folium Fici 75g, Herba Spirodelae 75g, Pericarpium Citri junoris 75g, 75% ethanol 250g, copper chloride 15g, Fructus Luffae water 750g.
The preparation method of vitiligo Masking sol of the present invention comprises following operating procedure:
1, Folium Fici, Herba Spirodelae, Pericarpium Citri junoris were soaked 60 minutes in water respectively, dry, be cut into filament, again with three kinds of abundant mix homogeneously of filament;
2,75% alcoholic solution is added in the filament of described mix homogeneously and soaked 48 hours; The elimination medicinal residues namely get pigment solution;
3, copper chloride is added in the described pigment solution, fully mix homogeneously; Add Fructus Luffae water again, fully mix homogeneously namely makes the about 1000ml of vitiligo Masking sol.
Embodiment 2:
Get Folium Fici 50g, Herba Spirodelae 50g, Pericarpium Citri junoris 50g, 75% ethanol 200g, copper chloride 10g, Fructus Luffae water 800g.
Other preparation process is with embodiment 1.Make the about 1000ml of vitiligo Masking sol.
Embodiment 3:
Get Folium Fici 100g, Herba Spirodelae 100g, Pericarpium Citri junoris 100g, 75% ethanol 300g, copper chloride 20g, Fructus Luffae water 700g.
Other preparation process is with embodiment 1.Make the about 1000ml of vitiligo Masking sol.
Embodiment 4:
Get Folium Fici 100g, Herba Spirodelae 50g, Pericarpium Citri junoris 50g, 75% ethanol 200g, copper chloride 15g, Fructus Luffae water 800g.
Other preparation process is with embodiment 1.Make the about 1000ml of vitiligo Masking sol.
Embodiment 5:
Get Folium Fici 50g, Herba Spirodelae 100g, Pericarpium Citri junoris 75g, 75% ethanol 300g, copper chloride 10g, Fructus Luffae water 700g.
Other preparation process is with embodiment 1.Make the about 1000ml of vitiligo Masking sol.
Embodiment 6:
Get Folium Fici 75g, Herba Spirodelae 100g, Pericarpium Citri junoris 50g, 75% ethanol 250g, copper chloride 20g, Fructus Luffae water 750g.
Other preparation process is with embodiment 1.Make the about 1000ml of vitiligo Masking sol.
The vitiligo Masking sol of making in order to the top method, on probation through 80 routine patients with vitiligo, male 44 examples wherein, women 36 examples, 33 years old mean age.Patient's course of disease is all more than 2 years, and average 8 years, topical type 48 examples wherein, acra type 25 examples are distributed type 7 examples, once use multiple medication effect not good.All patients put the white macula place outward on the skin with the present invention, put on the skin 1 time every 5 days, and 3 months is a course for the treatment of.The patient does not all use any other anti-vitiligo medicine during treating.Criterion of therapeutical effect: cure: white macula all disappears, and recovers the normal colour of skin; Produce effects: have the effect of the white macula of covering, white macula disappears with newborn pigment island all more than 50%; Effectively: have the effect of the white macula of covering, white macula disappears with newborn pigment island below 50%, more than 10%; Invalid: naked white macula effect, white macula no change or dwindle, the area that recovers the normal colour of skin accounts for the skin lesion area below 10%.The result shows, the equal produce effects of 80 routine patients with vitiligo, obvious effective rate 100%; The patient that 9 routine symptoms are lighter cures cure rate 11.25%.All patients untoward reaction such as skin irritation, allergy all do not occur during using the present invention.Therefore the present invention is a kind of comparatively ideal treating both the principal and secondary aspects of a disease type vitiligo Masking sol external preparation, and is safe and reliable.

Claims (3)

1. vitiligo Masking sol, it is characterized in that being made by the raw material of following weight portion: Folium Fici 50-100 part, Herba Spirodelae 50-100 part, Pericarpium Citri junoris 50-100 part, 75% ethanol 200-300 part, copper chloride 10-20 part, Fructus Luffae water 700-800 part, described Fructus Luffae water is the bleeding sap of the climing gap of Ramulus Luffae master.
2. vitiligo Masking sol according to claim 1 is characterized in that being made by the raw material of following weight portion: 75 parts of Folium Fici, 75 parts in Herba Spirodelae, 75 parts of Pericarpium Citri junoriss, 250 parts of 75% ethanol, 15 parts of copper chlorides, 750 parts in Fructus Luffae water.
3. the preparation method of vitiligo Masking sol is characterized in that comprising following operating procedure:
A, by the prescription of claim 1 Folium Fici, Herba Spirodelae, Pericarpium Citri junoris were soaked 60 minutes in water respectively, dry, be cut into filament, again with three kinds of abundant mix homogeneously of filament;
B, by the prescription of claim 1 75% alcoholic solution is added in the filament of described mix homogeneously and soaked 48 hours; The elimination medicinal residues namely get pigment solution;
C, by the prescription of claim 1 copper chloride is added in the described pigment solution, fully mix homogeneously; Prescription by claim 1 adds Fructus Luffae water again, and fully mix homogeneously namely gets the vitiligo Masking sol.
CN 201110197132 2011-07-14 2011-07-14 Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof Active CN102274359B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201110197132 CN102274359B (en) 2011-07-14 2011-07-14 Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201110197132 CN102274359B (en) 2011-07-14 2011-07-14 Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof

Publications (2)

Publication Number Publication Date
CN102274359A CN102274359A (en) 2011-12-14
CN102274359B true CN102274359B (en) 2013-08-07

Family

ID=45100304

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201110197132 Active CN102274359B (en) 2011-07-14 2011-07-14 Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof

Country Status (1)

Country Link
CN (1) CN102274359B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103181955A (en) * 2011-12-30 2013-07-03 张文知 Multi-functional spot-removing medicinal liquid
CN105055298A (en) * 2015-09-20 2015-11-18 南森 Novel leukoderma covering liquid
CN106344687A (en) * 2016-11-09 2017-01-25 徐峰 White spot repair liquid

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
《山东中草药手册》编写小组.山东人民卫生出版社.《山东中草药手册》.1970,(第一版),第757页倒数第2-3行. *
中西医结合治疗皮肤病 第8讲 白癜风的治疗.段行武.《中国临床医生》.2009,第37卷(第8期),第18-20页,参见第19页右栏倒数第7-8行.
段行武;中西医结合治疗皮肤病 第8讲 白癜风的治疗;《中国临床医生》;20091231;第37卷(第8期);第18-20页,参见第19页右栏倒数第7-8行 *
阮高波.非光敏中药组方外用治疗白癜风有效的机制研究.《中国优秀硕士学位论文全文数据库 医药卫生科技辑2009年第3期》.2009,正文第12页第16行、第39页第14-16行、、第42页第11-13行.
非光敏中药组方外用治疗白癜风有效的机制研究;阮高波;《中国优秀硕士学位论文全文数据库 医药卫生科技辑2009年第3期》;20090315;正文第12页第16行、第39页第14-16行、、第42页第11-13行 *

Also Published As

Publication number Publication date
CN102274359A (en) 2011-12-14

Similar Documents

Publication Publication Date Title
CN102028752B (en) Chinese herbal medicine compound freckle-removing and skin-whitening composition and external preparation
CN104644523B (en) A kind of medical bio smoothing wrinkle suppression spot dressing and preparation method thereof
KR100963792B1 (en) Shampoo containing composite for growing hair and preventing fall-out of hair
CN104434618A (en) Nine-herb cream with effects of whitening and removing freckle marks and preparation method thereof
CN103142456A (en) Traditional Chinese medicine acne removing face cream
CN100525745C (en) Spot-removing cream
CN102274359B (en) Vitiligo covering liquid treating both manifestation and root cause and preparation method thereof
CN101721347A (en) Beauty skin care product containing artificially-cultured giant salamander tissue substance or extract
CN105853276A (en) Natural plant formula for hair blacking and white hair delaying
CN103417462B (en) Facial mask for treating whelk and pigment patch and whitening skin
CN102335342B (en) Chinese medicinal composition for treating chloasma, preparation method and application thereof
CN102228415B (en) Composition containing tranexamic acid, and preparation method and uses thereof
CN105213279A (en) A kind of body lotion eliminating striae atrophicae and preparation method thereof
CN105395358A (en) Moxa thermal moxibustion beauty mask
KR20130054681A (en) A hair restorer method
CN102362842A (en) Preparation and application of traditional Chinese medicine compound extract with whitening and beverage removing effects
CN104324367A (en) Novel application of fish scale extract in preparation of medicine for treating and/or preventing leukoderma
CN106390061A (en) Hair follicle stem cell awakening factor composition for external use, hair loss prevention, and hair regeneration, and preparation method thereof
CN102847030A (en) Freckle-removal facial mask
CN102846500A (en) Leucoderma skin color covering liquid and preparation method thereof
CN109745284A (en) A kind of essence facial mask and preparation method thereof
KR101040011B1 (en) Demodex folliculorum germicide
CN108542847A (en) It is a kind of to dispel acne, furuncle and composition and its application of going acne print
RU2802005C1 (en) Cosmetic mask and a method of its use
CN101862384B (en) External medicament for treating leucoderma

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB03 Change of inventor or designer information

Inventor after: Lei Hong

Inventor after: Wang Qing

Inventor before: Wang Qing

Inventor before: Lei Hong

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: WANG QING LEI HONG TO: LEI HONG WANG QING

C14 Grant of patent or utility model
GR01 Patent grant
CP03 Change of name, title or address
CP03 Change of name, title or address

Address after: No. 105 Zimei Road, Zhongxin Suzhou Chuhai High tech Industrial Development Zone, Chuzhou City, Anhui Province 239065 Wusuwei Biopharmaceutical Co.,Ltd.

Patentee after: Wang Qing

Country or region after: China

Address before: Room 151, Building 72, Shiyou San Village, Qixia District, Nanjing City, Jiangsu Province, 210035

Patentee before: Wang Qing

Country or region before: China