CN102362842A - Preparation and application of traditional Chinese medicine compound extract with whitening and beverage removing effects - Google Patents
Preparation and application of traditional Chinese medicine compound extract with whitening and beverage removing effects Download PDFInfo
- Publication number
- CN102362842A CN102362842A CN201110291367XA CN201110291367A CN102362842A CN 102362842 A CN102362842 A CN 102362842A CN 201110291367X A CN201110291367X A CN 201110291367XA CN 201110291367 A CN201110291367 A CN 201110291367A CN 102362842 A CN102362842 A CN 102362842A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- extraction
- ethanol
- medicine compound
- compound extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a traditional Chinese medicine compound extract with whitening and beverage removing effects. The traditional Chinese medicine compound extract is an extract prepared by using ten kinds of traditional Chinese medicine such as patrinia villosa juss, shinyleaf yellowhorn kernels, cortex dictamni, hairyvein agrimony, rhodiola root, cassia seeds, semen boitae, golden buckwheat rhizome, peppermint, liquorice and the like as raw materials and adopting the carbon dioxide supercritical extraction technology and the macroporous absorbent resin enrichment technology at certain technological parameters. The extract has an obvious tyrosinase activity inhibition effect and a melanin generation inhibition effect and can be directly and safely applied to the preparation of cosmetics and external use medicine preparations with the whitening and beverage removing effects.
Description
Technical field
The present invention relates to a kind of Chinese medicine compound extract; Specifically relate to a kind of employing carbon dioxide supercritical fluid extraction technology and macroporous adsorbent resin beneficiation technologies; With patrima villosa grass, Lignum Xanthoceratis kernel, Cortex Dictamni, Herba Agrimoniae, Radix Rhodiolae, Semen Cassiae, Semen Platycladi, Rhizoma Fagopyri Dibotryis, Herba Menthae, Radix Glycyrrhizae is the extract that raw material makes, and this extract has tangible tyrosinase activity inhibitory action and melanin generates inhibitory action.The invention also relates to the preparation of the extract of the drug classes eradicates whitening preparations, health foods and cosmetics applications.
Background technology
Loving beauty is part of human nature.Along with growth in the living standard, people require increasingly highly to quality of life, more and more pay attention to whitening naturally of skin, and facial area seem particularly important.Chloasma is a kind of acquired pigmentation dermatosis, is to be changed by the limitation due to the multiple reason, scrambling skin pigment, and clinical manifestation is the symmetry pigmentation; Be the butterfly aliform; The lighter is yellowish or light brown, and the some lamellar intersperses among the buccal both sides, and the outside is multiple down with eye; Weight person is brown deeply or light/dark balance is distributed in face.Primary disease is mainly in young and middle-aged women, does not generally have any subjective symptoms, and the course of disease is obstinate, and it is attractive in appearance to have a strong impact on the patient, brings many worries and misery for patient's spirit and life aspect.
The chloasma pathogeny is complicated, and melanocyte too much is its core pathological manifestations.Its definite pathogenesis is not bright so far; It is generally acknowledged relevantly with many factors such as melanocyte Developmental and Metabolic Disorder, endocrine disturbance, gestation, oral contraceptive, inherited genetic factors, oxygen-derived free radicals, ultraviolet radiation, tryrosinase dysfunctions, wherein the skin melanocyte Developmental and Metabolic Disorder that causes such as endocrine disturbance, heredity, ultraviolet radiation is the main cause of its morbidity.
Modern medicine treatment chloasma mainly comprises uses lucifuge agent (like zinc oxide, titanium dioxide etc.), and system uses vitamins, tranamic acid, melatonin etc.; But be main with the local topical chemical decolorization basically, like sterin in hydrogen ketone, Azelaic Acid, kojic acid, glutathion, indometacin, retinoic acid, phenolic compound, the cortex etc.; Also having chemistry to strip off horny layer or chemical skin sand grinds off except that melanocyte and with dye laser and destroys melanin granule etc., most methods is taken off toxic and side effects or unstable, easy recurrence of slow curative effect itself such as mistake, telangiectasis, contact dermatitis because of causing inhomogeneous pigment, and the fraud that causes permanent decolouring and medicine source property moth patch is arranged, and fails to apply.
Chinese medicine is called chloasma " chloasma ", " dusty face ", " chloasma hepaticum " etc., the Golden Mirror of Medicine cloud: " dim for a long time idle dark like dirt, former form in the troubled thoughts depression ".To the symptom of primary disease cloud again: " first improvement such as dust and dirt, with the passing of time black sapropelitic shape is withered secretly not damp, not of uniform size.Little person such as foxtail millet grain Semen Phaseoli, big person is like Semen Nelumbinis or long or oblique or circle.Equal with skin, the women has it more ".Think the liver,kidney,spleen functional disorder, QI-blood circulation is not normal, makes the venation inanition can not go up China in face, or the turbid resistance network of the stasis of blood, and penting up skin is the main cause of chloasma morbidity.
The Chinese medicine chloasma is stressed internal organs negative and positive of qi and blood balance, pays attention to regulating functions of ZANG FU-organs, controls with blood circulation promoting and blood stasis dispelling dispersing the stagnated live-QI to relieve the stagnation of QI, nourishing the liver and kidney, strengthening spleen and nourishing blood.Or treatment by oral administration of medicines is main or external treatment is main, or interior external treatment and usefulness.Chinese medicine treatment of melasma long history, as early as in the "Shen Nong's Herbal Classic", "doctors do not record", "Tai Ping Sheng Hui Fang", "Qian Jin Fang" and other classical medical books clearly documented the ability to "eradicates face black, good color." prescriptions.A large amount of clinical practices prove that the Chinese medicine chloasma demonstrates certain advantage, have that outstanding integrally-regulated, stable curative effect, side effect are little, the patient is easy to advantages such as acceptance, and curative effect is better with western medicine than merely.But the treatment by Chinese herbs chloasma exists still that slow curative effect, cure rate are not high, the course of treatment is long, take shortcoming such as Chinese medicine inconvenience for a long time, has influenced the compliance of treatment.
In a word; The medicine and the means of integrative therapy chloasma are many; But or because of curative effect too slowly or too many because of untoward reaction, do not have always a kind of evident in efficacy, safely, the therapeutic scheme of generally acknowledging, make doctors and patients all to be satisfied with, this needs the utmost point not conform to clinical practice.Therefore, seek the Chinese prescription of reasonable recipe, determined curative effect, and be prepared into efficient, safe Chinese medicine whitening and spot-eliminating extract, become a research focus of present Chinese medicine beauty treatment fields through the modern Chinese medicine extractive technique.
Summary of the invention
An object of the present invention is to provide a kind of tyrosinase activity inhibitory action and melanin and generate inhibiting Chinese medicine compound extract with excellence.
Another object of the present invention is to propose the application of this Chinese medicine compound extract in pharmaceutical preparation, health food and the cosmetics of preparation whitening and spot-eliminating.
In order to achieve the above object, the present invention has taked following technical scheme:
Prescription medical material and ratio are: in parts by weight, and 5 parts of patrima villosa grass, 5 parts of Lignum Xanthoceratis kernel, 3 parts of Cortex Dictamni, 3 parts of Herba Agrimoniaes, 3 parts of Radix Rhodiolaes, 3 parts of Semen Cassiaes, 3 parts of Semen Platycladi, 3 parts of Rhizoma Fagopyri Dibotryiss, 2 parts of Herba Menthaes, 1 part in Radix Glycyrrhizae.
In parts by weight, 5 parts of patrima villosa grass, 5 parts of Lignum Xanthoceratis kernel, 3 parts of Cortex Dictamni, 3 parts of Herba Agrimoniaes, 3 parts of Radix Rhodiolaes, 3 parts of Semen Cassiaes, 3 parts of Semen Platycladi, 3 parts of Rhizoma Fagopyri Dibotryiss, 2 parts of Herba Menthaes, 1 part in Radix Glycyrrhizae, it is an amount of to take by weighing each medical material; Mix and pulverize, cross No. 2 sieves of pharmacopeia, medicated powder is packed in the carbon dioxide supercritical fluid extraction device; Add the entrainer dehydrated alcohol, consumption is a 0.5ml/g medicated powder, and setting extraction temperature is 45 ℃; Extracting pressure is 40MPa, and resolving I pressure is that 10MPa, temperature are 40 ℃, and resolving II pressure is that 4MPa, temperature are 25 ℃; The extraction time is 2h, gets pale brown color thickness grease.Medicinal residues with behind the last step carbon dioxide supercritical fluid extraction added 10 times of amount 70% soak with ethanol 12 hours, heating and refluxing extraction 2 hours; Filter, in medicinal residues, add 5 times of amount 70% alcohol heating reflux again and extracted 2 hours, filter; Merging filtrate, drying under reduced pressure gets extractum.Extractum is added suitable quantity of water dilution back to be mixed with isopyknic D-101 type macroporous adsorbent resin and mixes thoroughly; Be splined in the D-101 type macroporous adsorptive resins (applied sample amount is calculated as 1: 2 with the ratio of crude drug amount and amount of resin); Static adsorption 30 minutes is carried out eluting with 20% ethanol of the water of 10 column volumes, 10 column volumes and 50% ethanol of 10 column volumes then successively, collects 50% ethanol elution; Pulverize behind the drying under reduced pressure, make brown ceramic powder.The pale brown color thickness grease that this powder and carbon dioxide supercritical fluid extraction are made mixes, and promptly gets Chinese medicine compound extract of the present invention.
As to the improvement of technique scheme with replenish:
1, the relative quantity of prescription Chinese crude drug can be adjusted according to the ratio of: patrima villosa grass 4-6 part, Lignum Xanthoceratis kernel 4-6 part, Cortex Dictamni 2-4 part, Herba Agrimoniae 2-4 part, Radix Rhodiolae 2-4 part, Semen Cassiae 2-4 part, Semen Platycladi 2-4 part, Rhizoma Fagopyri Dibotryis 2-4 part, Herba Menthae 1-3 part, Radix Glycyrrhizae 1-2 part.
2, when carrying out carbon dioxide supercritical fluid extraction, extraction temperature can be set at 30-60 ℃.
3, when carrying out carbon dioxide supercritical fluid extraction, extracting pressure can be set at 20-60MPa.
4, when carrying out carbon dioxide supercritical fluid extraction, the extraction time can be set at 1h or more than.
5, when carrying out carbon dioxide supercritical fluid extraction, entrainer can be the ethanol more than 95% for ethanol or concentration, and consumption can be set at 0.1-1ml/g medicated powder.
6, when with Rotary Evaporators carbon dioxide supercritical fluid extraction liquid being carried out concentrating under reduced pressure recovery ethanol, temperature can be set at 45 ℃ or following.
7, when the medicinal residues behind the carbon dioxide supercritical fluid extraction are extracted, extracting solvent can be 30%-100% ethanol.
8, when the medicinal residues behind the carbon dioxide supercritical fluid extraction are extracted, extracting mode can be that heating and refluxing extraction, supersound extraction or percolation extract.
9, when ethanol extraction extractum is carried out the macroporous adsorbent resin enrichment, the post fat of use can be D-101 type or AB-8 type, and other are nonpolar, low pole or middle polarity macroporous adsorbent resin.
10, when ethanol extraction extractum is carried out the macroporous adsorbent resin enrichment, eluting solvent can be water or Different concentrations of alcohol, according to concentration of alcohol eluting successively from low to high.
Through the extract that technique scheme prepares, have tangible tyrosinase activity inhibitory action and melanin and generate inhibitory action, can be used to prepare pharmaceutical preparation, health food and the cosmetics of whitening and spot-eliminating.
When the mixing of the extract into a whitening effect of the drug formulation depigmentation and health food, except these extracts, but does not prevent the effects of the present invention, may be appropriately mixed and used for general care pharmaceutical preparations the other ingredients in the food, such as: cross-linked polyvinyl pyrrolidone, sodium carboxymethyl starch, microcrystalline cellulose, lactose, a Division TAZ, materials with added magnesium stearate, plus materials and coating materials.Its type agent can be any pharmaceutically said dosage form, preferred pill, tablet, capsule or oral liquid.
When mixing the above-mentioned extract into the role of a whitening cosmetic depigmentation and topical pharmaceutical preparations, in addition to these extracts, but does not prevent the effects of the present invention, mixing may be appropriately used for general cosmetics, pharmaceuticals other skin external agent for the other components, for example: oil, wetting agents, ultraviolet absorbers, antioxidants, surfactants, preservatives, humectants, fragrances, water, alcohol, and thickeners.Extract of the present invention can be used for cosmetics and external medicine preparation, is particularly suitable for being widely used in the cosmetics.Its dosage form is so long as be used for the dosage form of skin and get final product, applicable to solution system, can dissolve dosage form arbitrarily such as system, emulsification system, ointment, gel.In addition, it uses form also is arbitrarily, for example: astringent, emulsion, frost, facial film etc.
The pure natural plants extract that Chinese medicine compound extract of the present invention is a dietotherapeutic, effect experiment subsequently will prove that Chinese medicine compound extract of the present invention has the tyrosinase activity inhibitory action and melanin generates the effect that suppresses.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.These embodiment are interpreted as only being used to the present invention is described and are not used in restriction protection scope of the present invention.After the content of having read the present invention's record, those skilled in the art can do various changes or modification to the present invention, and these equivalences change and modify and fall into claims of the present invention institute restricted portion equally.For convenience of explanation, abbreviate extract of the present invention as TQW below.
Embodiment 1 (experimentation that the TQW restraint of tyrosinase is active and melanin generates)
1 experiment material
Mice B16 melanoma cells (purchasing Shanghai cell institute) in the Chinese Academy of Sciences; Arbutin (purchasing company) in Sigma; DMEM culture medium (purchasing company) in Gibco; Hyclone (purchasing company) in Billab; Trypsin is purchased the company in Billab); Phosphate-buffered salt (purchasing China fir Golden Bridge company limited in Beijing); Two anti-(purchasing company) in Billab; DOPA (purchasing company) in Sigma; TritonX-100 (purchasing company) in Sigma; MTT (purchasing company) in Billab; DMSO (purchasing company) in Sigma; ELIASA (thermoelectric Shanghai Instr Ltd.).
The preparation of 2 reagent
Getting arbutin faces the time spent and is diluted to the experiment desired concn with the DMEM culture medium.
Get TQW, use a small amount of anhydrous alcohol solution, the dissolving of reuse DMEM culture medium is made into 1mg/ml, and wherein concentration of alcohol is 0.8%, and regulating pH value is 7.0, filtration sterilization, and 4 ℃ of preservations, subsequent use.
3 cell culture and exposed by the reagent thing
Get mice B16 melanoma cells, treat that cell grows to nearly fusion state,, go down to posterity, place CO with the DMEM culture fluid that contains 10% calf serum through 0.25% trypsinization
237 ℃ of incubators, 5%CO
2Cultivate in the saturated humidity environment.Same passage cell is taken from experiment each time.
3.1 experiment is divided into groups
Blank control group is in the hole of inoculating cell not, to add cell culture fluid; Negative control group is in the hole of having inoculated cell, to add cell culture fluid; The administration group is in the hole of having inoculated cell, to add the culture fluid that contains variable concentrations TQW respectively; The arbutin group is in the hole of having inoculated cell, to add the culture fluid that contains the variable concentrations arbutin respectively.
3.2 cell proliferation inhibition rate is measured
Selection grows to the cell of nearly fusion state, uses 0.25% trypsinization, and the adjustment cell concentration is with every hole 5 * 10
3Individual 96 orifice plates that are inoculated in; Amount of liquid 150 μ L in every hole place 37 ℃, and volume fraction is after cultivating 24h under the 5%CO2 condition; Negative control group is changed liquid 1 time with fresh medium; Administration group and arbutin group receive the fresh medium of reagent thing to change liquid 1 time with adding, and arbutin, TQW concentration are established 10 μ g/ml, 50 μ g/ml, 100 μ g/ml respectively, and each concentration is established 12 multiple holes; Blank control group only adds culture fluid and does not add cell; Each group all places 37 ℃, and volume fraction is 5%CO
2Cultivate 72h under the condition, 4h adds MTT 20 μ L/ holes before finishing, and hatches abandoning supernatant behind the 4h for 37 ℃, adds dimethyl sulfoxide (DMSO) 150 μ L/ holes, vibrates about 10min.Survey the OD value with ELIASA, wavelength is 490nm.
Cell proliferation inhibition rate %=[1-(administration group OD value-blank control group OD value)/(negative control group OD value-blank control group OD value)] * 100%
3.3 the tyrosinase activity suppression ratio is measured
Selection grows to the cell of nearly fusion state, uses 0.25% trypsinization, and the adjustment cell concentration is with every hole 5 * 10
3Individual 96 orifice plates that are inoculated in; Amount of liquid 150 μ L in every hole place 37 ℃, and volume fraction is after cultivating 24h under the 5%CO2 condition; Negative control group is changed liquid 1 time with fresh medium; Administration group and arbutin group receive the fresh medium of reagent thing to change liquid 1 time with adding, and arbutin, TQW concentration are established 10 μ g/ml, 50 μ g/ml, 100 μ g/ml respectively, and each concentration is established 12 multiple holes; Blank control group only adds culture fluid and does not add cell; Each group all places 37 ℃, and volume fraction is to cultivate 72h under the 5%CO2 condition, and the culture fluid that inclines is washed twice with PBS; Add TritonX-10090 μ L/ hole, concussion 30min adds 0.2%L-dopa solution 60 μ L/ holes; Hatch 20min for 37 ℃, survey the OD value with ELIASA, wavelength is 490nm.
Tyrosinase activity suppression ratio %=[1-(administration group OD value-blank control group OD value)/(negative control group OD value-blank control group OD value)] * 100%
3.4 the melanin content suppression ratio is measured
Select the exponential phase cell, use 0.25% trypsinization, the adjustment cell concentration is inoculated in the culture bottle, places 37 ℃, and volume fraction is 5%CO
2After cultivating 24h under the condition; Negative control group is changed liquid 1 time with fresh medium; Administration group and arbutin group receive the fresh medium of reagent thing to change liquid 1 time with adding, and arbutin, TQW concentration are established 10 μ g/ml, 50 μ g/ml, 100 μ g/ml respectively, and each concentration is established 12 multiple holes; Blank control group only adds culture fluid and does not add cell; Each group all places 37 ℃, and volume fraction is 5%CO
2Cultivate 72h under the condition, with 0.25% pancreatin harvesting, centrifugal (1500r/min, 5min) back abandoning supernatant adds the PBS buffer and regulates cell density to 10
5About/ml, each is drawn 1ml cell suspension and places 3 parallel tool plug pipes, centrifugal (1500r/min respectively; 5min) back abandoning supernatant adds 200 μ l distilled water earlier cell is suspended again, and add 1ml ethanol then: ether solution (volume ratio is 1: 1) is to dissolve the opaque granule of non-melanocyte; At room temperature place 15min; It is centrifugal that (3000r/min 5min) and abandoning supernatant, adds the 1ml mass fraction and is 10% dimethyl sulfoxide, 1mol/LNaOH solution; Place 80 ℃ of water-bath 30min, cell mass is dissolved fully.Survey the OD value with ELIASA, wavelength is 490nm.
Melanin content suppression ratio %=[1-(administration group OD value-blank control group OD value)/(negative control group OD value-blank control group OD value)] * 100%
4 interpretations of result
4.1TQW influence to the growth of B-16 cell
Visible by table 1, the inhibitory action of TQW cell growth with compare with the concentration arbutin, the equal not statistically significant of difference (P＜0.05) explain that the cell growth inhibition of TQW is similar with traditional whitening agent arbutin, and cytotoxicity is lighter.
4.2TQW to B-16 cell tyrosinase activity and the synthetic influence of melanocyte
By table 2,3 visible; Three concentration of TQW all have the active and synthetic effect of melanocyte of significant restraint of tyrosinase; And effect all is better than arbutin, explain restraint of tyrosinase is active synthesize with melanocyte aspect, TQW has more significantly than traditional whitening agent arbutin and acts on.
Table 1TQW is to mice B16 melanoma cells inhibition of proliferation rate (
%)
With compare with the concentration arbutin,
△P＜0.05,
△ △P＜0.01
Table 2TQW is to the suppression ratio (
%) of mice B16 melanoma cells tyrosinase activity
Compare with negative control,
*P＜0.05,
*P＜0.01; With compare with the concentration arbutin,
△P＜0.05,
△ △P＜0.01
Table 3TQW is to the synthetic suppression ratio of mice B16 melanoma cells melanocyte (
%)
Compare with negative control,
*P＜0.05,
*P＜0.01; With compare with the concentration arbutin,
△P＜0.05,
△ △P＜0.01
Embodiment 2 (TQW induces the Pigmented inhibitory action experimentation of guinea pig skin to UVB)
1 experimental apparatus, reagent and laboratory animal
UVB ultraviolet Phototherapeutic instrument (Sigma company); 0.1%8-methoxypsoralen (8-MOP) tincture (Sigma company); VITAMIN C TABLET (Shijiazhuang Ouyi Pharmaceutical Co., Ltd); 90 of the healthy pure white Cavia porcelluss of regular grade, female, body weight 250-320g; Prepare the external used medicine that contains variable concentrations TQW with common substrate, contain the filling stomach medicine of variable concentrations TQW with the preparation of distilled water suspendible.
Inductive pigmentation model copy of 2UVB and administration
Every guinea pig back is got 3 phase abscission zone territory (2 * 2cm
2), to wipe out the guinea pig back centre with curved scissors and become mildewed, electric shaver is shaved clean undercoat, uses the irradiation of SS-04B type ultraviolet phototherapy appearance, Burdick lamp configuration UVB light source, the spectrum peak of fluorescent tube is 310～315nm; Pre-irradiation is used the UVB ultraviolet radiation meter and is measured the irradiation light intensity, and irradiation should zone 20min, every day irradiation dose 200mJ/cm
2, continuous 2 weeks, cumulative exposure 2800mJ/cm
2Each irradiation administration in back 2 hours, in 2 weeks of end of radiation continued administration, the bark fetching skin tissue is done pathologic finding.
3 divide into groups
3.1 external experiment
Make blank with the normal guinea pig skin of back that does not shine ultraviolet for the 1st group; The 2nd group, modeling is coated with the employed substrate of preparation external used medicine outward, as own control; 3 and 4 groups, modeling is coated with the medicine that contains TQW outward, and concentration is respectively 0.5 and 1mg/ml; The 5th group, modeling is coated with 0.1%8-methoxypsoralen (8-MOP) tincture outward, as positive control.
3.2 experiment for oral administration
Make blank with the normal guinea pig skin of back that does not shine ultraviolet for the 1st group; The 2nd group, modeling is irritated stomach and is given distilled water 2ml, as own control; 3 and 4 groups, the medicine that stomach contains TQW is irritated in modeling, dosage be 10 with the 20mg/kg body weight; The 5th group, modeling is irritated stomach and is given vitamin C, and dosage is the 20mg/kg body weight, as positive control.
4 colouring methods
4.1HE dyeing: (2cm * 2cm), routine paraffin wax section HE dyes from guinea pig back bark fetching skin BIAO and BEN.
4.2Schmorl method dyeing
(1) section moves to washing.(2) with 0.037mol/L (1%) the iron chloride 30ml of fresh configuration, the mixed liquor of the 0.03mol/L of fresh configuration (1%) potassium ferricyanide 4mL and distilled water 6mL is handled 10min.(3) flowing water cleans.The result: melanocyte dyeing is yellowish-brown, black particle.
4.3Imokawa method dyeing
(1) (2cm * 2cm), with 0.1mol/LPBS liquid (pH6.8) flushing, the 1mol/L sodium bromide is hatched 5h for 37 ℃ from guinea pig back bark fetching skin BIAO and BEN.(2) separate epidermis, corium.(3) epidermis is with the fixing 30min of the cold neutral formalin of 3.33mol/L (10%), with 0.1mol/LPBS liquid (pH6.8) flushing 2 times.(4) with (pH6.8) 5h that dyes of 0.1% DOPA (0.1mol/LPBS liquid).The result: melanocyte is dyed pitchy.
5 om observations
5.1 contain the melanin granule cell counting: the dyeing of Schmorl method, high power lens contain the melanin granule cell number with every square millimeter of stratum basale of the blind method every part of BIAO and BEN of counting of net form eyepiece micrometer list down.
5.2 DOPA positive cell counting: the dyeing of Imokawa method, high power lens contain the dopa-positive melanocytes number with every square millimeter of stratum basale of the blind method every part of BIAO and BEN of counting of net form eyepiece micrometer list down.
6 interpretations of result
6.1 external experiment
Visible by table 4, to compare with blank control group, UVB model group guinea pig skin contains the melanin granule cell, DOPA positive cell number average obviously increases, and significant difference (P＜0.01) is arranged, the inductive pigmentation skin model success that is similar to chloasma of this explanation UVB.TQW can obviously reduce guinea pig skin and contain melanin granule cell, DOPA positive cell number, and the effect of high concentration group is better than positive control drug 8-MOP.Explain that extract of the present invention has good Elder.
Table 4 external TQW is to the influence
of guinea pig skin melanocyte and melanocyte formation
| Group | Contain melanin granule cell/mm 2 | The positive MC/mm of DOPA 2 |
| Blank control group | 367.21±14.73 △△ | 169.43±11.02 △△ |
| The UVB model group | 476.01±21.40 ** | 240.12±12.58 ** |
| The TQW0.5mg/ml group | 419.09±15.26 **△△ | 201.67±8.43 **△△ |
| TQW 1mg/ml group | 370.68±16.20 △△ | 183.06±9.98 △△ |
| The 8-MOP group | 380.34±15.23 *△△ | 187.42±8.87 *△△ |
Compare with blank control group
*P＜0.05,
*P＜0.01; Compare with the UVB model group
△P＜0.05,
△ △P＜0.01
6.2 experiment for oral administration
Visible by table 5, to compare with blank control group, UVB model group guinea pig skin contains the melanin granule cell, DOPA positive cell number average obviously increases, and significant difference (P＜0.01) is arranged, the inductive pigmentation skin model success that is similar to chloasma of this explanation UVB.TQW can obviously reduce guinea pig skin and contain melanin granule cell, DOPA positive cell number, and the effect of high concentration group is better than the positive control drug vitamin C.Explain that extract of the present invention has good Elder.
Table 5 TQW for oral administration is to the influence
of guinea pig skin melanocyte and melanocyte formation
| Group | Contain melanin granule cell/mm 2 | The positive MC/mm of DOPA 2 |
| Blank control group | 367.21±14.73 △△ | 169.43±11.02 △△ |
| The UVB model group | 476.01±21.40 ** | 240.12±12.58 ** |
| The TQW10mg/kg group | 429.3±14.35 **△△ | 215.76±8.41 **△△ |
| The TQW20mg/kg group | 375.74±12.27 △△ | 172.16±9.28 △△ |
| Vitamin C 20mg/kg group | 380.87±11.38 △△ | 176.09±10.41 △△ |
Compare with blank control group
*P＜0.05,
*P＜0.01; Compare with the UVB model group
△P＜0.05,
△ △P＜0.01
Embodiment 3 (preparation of tablet)
By weight, get TQW5～9 part, add 1～2 part of Pulvis Talci, 1～2 part of starch, 1～2 part of carboxymethyl starch sodium sprays into 95% ethanol moistening, wet granulation, drying adds 0.5～1% magnesium stearate, mix homogeneously, tabletting; Add 15% Opadry coating solution coating, 7% coating that increases weight gets Film coated tablets.
Embodiment 4 (preparation of capsule)
By weight, get TQW8～9 part, add 1～2 part of Pulvis Talci, spray into 95% ethanol moistening, wet granulation, drying adds 0.5～1% magnesium stearate, mix homogeneously, packing gets capsule.
Embodiment 5 (preparation of facial cream)
Preparation technology: (04) to (07) is mixed and heated to 80 ℃, adds (09), (10) to whole dissolvings, continuation adds (01), (02), oil phase is processed in (03) and (08); (11) to (13) are added (14), be heated to 80 ℃ and process water, behind the biphase mixing and emulsifying, be cooled to adding (15) below 50 ℃, mixing gets final product.
Claims (10)
1. the Chinese medicine compound extract of a Pear Power effect of dispelling spots, its method for distilling is following:
In weight portion; According to patrima villosa grass 4-6 part, Lignum Xanthoceratis kernel 4-6 part, Cortex Dictamni 2-4 part, Herba Agrimoniae 2-4 part, Radix Rhodiolae 2-4 part, Semen Cassiae 2-4 part, Semen Platycladi 2-4 part, Rhizoma Fagopyri Dibotryis 2-4 part, Herba Menthae 1-3 part, Radix Glycyrrhizae 1-2 part, it is an amount of to take by weighing each medical material, mixes and pulverizes; Cross No. 2 sieves of pharmacopeia; Medicated powder carries out carbon dioxide supercritical fluid extraction, is entrainer with ethanol, under the certain process parameter, extract pale brown color liquid; Concentrating under reduced pressure reclaims ethanol, final pale brown color thickness grease; To go up the medicinal residues of step behind the carbon dioxide supercritical fluid extraction again, with extracting after the debita spissitudo soak with ethanol, extracting solution is dry must extractum, and extractum utilizes macroporous adsorptive resins to carry out enrichment, under the certain process parameter, makes brown ceramic powder; The pale brown color thickness grease that this powder and carbon dioxide supercritical fluid extraction are made mixes, and promptly gets Chinese medicine compound extract of the present invention.
2. Chinese medicine compound extract according to claim 1; It is characterized in that extraction temperature is 30-60 ℃ when the medicated powder of spending Herba Patriniae, Lignum Xanthoceratis kernel, Cortex Dictamni, Herba Agrimoniae, Radix Rhodiolae, Semen Cassiae, Semen Platycladi, Rhizoma Fagopyri Dibotryis, Herba Menthae, Radix Glycyrrhizae in vain is carried out carbon dioxide supercritical fluid extraction, extracting pressure is 20-60MPa; The extraction time be 1h or more than; Entrainer dehydrated alcohol consumption is a 0.1-1ml/g medicated powder, and resolving pressure is 20MPa or following, and resolution temperature is 40 ℃ or following.
3. Chinese medicine compound extract according to claim 1, when it is characterized in that the medicinal residues behind the carbon dioxide supercritical fluid extraction are extracted, extracting solvent is 30%-100% ethanol; Consumption is 10 times of amounts for the first time, soaks 12 hours, and heating and refluxing extraction or supersound extraction or percolation extracted 2 hours; Filter, in medicinal residues, add 5 times of amount 30%-100% ethanol again, extracted again 2 hours; Filter, merging filtrate, drying under reduced pressure gets extractum.
4. Chinese medicine compound extract according to claim 1 is characterized in that when ethanol extraction extractum is carried out the macroporous adsorbent resin enrichment, extractum is added mix thoroughly back the mixing with isopyknic D-101 type macroporous adsorbent resin of suitable quantity of water dilution; Be splined on D-101 type or AB-8 type; And other are nonpolar, in low pole or the middle polarity macroporous adsorptive resins, applied sample amount is calculated as 1: 2, static adsorption 30 minutes with the ratio of crude drug amount and amount of resin; Carry out eluting with the Different concentrations of alcohol of 10 column volumes successively then; Collect the 20%-50% ethanol elution, pulverize behind the drying under reduced pressure, make brown ceramic powder.
5. Chinese medicine compound extract according to claim 2; It is characterized in that when carrying out carbon dioxide supercritical fluid extraction extraction temperature is 45 ℃, extracting pressure is 40MPa; The extraction time is 2h; Entrainer dehydrated alcohol consumption is a 0.5ml/g medicated powder, and resolving I pressure is that 10MPa, temperature are 40 ℃, and resolving II pressure is that 4MPa, temperature are 25 ℃.
6. Chinese medicine compound extract according to claim 3, when it is characterized in that the medicinal residues behind the carbon dioxide supercritical fluid extraction are extracted, extracting mode is that heating and refluxing extraction, supersound extraction or percolation extract.
7. Chinese medicine compound extract according to claim 4; When it is characterized in that ethanol extraction extractum carried out the macroporous adsorbent resin enrichment; The post fat that uses is D-101 type or AB-8 type, and other are nonpolar, low pole or middle polarity macroporous adsorbent resin.
8. Chinese medicine compound extract according to claim 4, when it is characterized in that ethanol extraction extractum carried out the macroporous adsorbent resin enrichment, eluting solvent is water or Different concentrations of alcohol, according to concentration of alcohol eluting successively from low to high.
9. Chinese medicine compound extract according to claim 4; When it is characterized in that ethanol extraction extractum carried out the macroporous adsorbent resin enrichment; Extractum is added suitable quantity of water dilution back mix with isopyknic D-101 type macroporous adsorbent resin and mix thoroughly, be splined in the D-101 type macroporous adsorptive resins (applied sample amount is calculated as 1: 2 with the ratio of crude drug amount and amount of resin), static adsorption 30 minutes; Carry out eluting with 20% ethanol of the water of 10 column volumes, 10 column volumes and 50% ethanol of 10 column volumes successively then; Collect 50% ethanol elution, pulverize behind the drying under reduced pressure, make brown ceramic powder.
10. any one described Chinese medicine compound extract is used in the pharmaceutical preparation of the class of dispelling of whitening, health food and cosmetics among the claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110291367XA CN102362842B (en) | 2011-09-30 | 2011-09-30 | Preparation and application of traditional Chinese medicine compound extract with whitening and freckle removing effects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110291367XA CN102362842B (en) | 2011-09-30 | 2011-09-30 | Preparation and application of traditional Chinese medicine compound extract with whitening and freckle removing effects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102362842A true CN102362842A (en) | 2012-02-29 |
| CN102362842B CN102362842B (en) | 2013-07-10 |
Family
ID=45689459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110291367XA Expired - Fee Related CN102362842B (en) | 2011-09-30 | 2011-09-30 | Preparation and application of traditional Chinese medicine compound extract with whitening and freckle removing effects |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102362842B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105395442A (en) * | 2016-01-11 | 2016-03-16 | 北京华信龙悦科技有限公司 | Natural plant long-lasting moisturizing composition and use thereof |
| CN106511213A (en) * | 2016-12-24 | 2017-03-22 | 张涉应 | Cosmetic containing plant extract liquid |
| CN107669533A (en) * | 2016-12-12 | 2018-02-09 | 青岛大学 | A kind of preparation method of UV white whitening toner |
| CN108014059A (en) * | 2016-11-04 | 2018-05-11 | 无锡法瑞雅生物细胞科学有限公司 | A kind of skin whitening, moisturizing lotion and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145229A (en) * | 1995-09-09 | 1997-03-19 | 梁根弟 | Method for production of cleaning powder for treating internal organs of the body |
| CN101468171A (en) * | 2007-12-28 | 2009-07-01 | 何顺洪 | Medicament for weight-reducing of fat people and weight-gaining of thin people |
| CN101537154A (en) * | 2008-09-12 | 2009-09-23 | 罗春青 | Manufacturing method of medicament for treating ten kinds of diseases |
| CN101554462A (en) * | 2008-04-08 | 2009-10-14 | 陈星儒 | Medicament for curing cancer |
-
2011
- 2011-09-30 CN CN201110291367XA patent/CN102362842B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145229A (en) * | 1995-09-09 | 1997-03-19 | 梁根弟 | Method for production of cleaning powder for treating internal organs of the body |
| CN101468171A (en) * | 2007-12-28 | 2009-07-01 | 何顺洪 | Medicament for weight-reducing of fat people and weight-gaining of thin people |
| CN101554462A (en) * | 2008-04-08 | 2009-10-14 | 陈星儒 | Medicament for curing cancer |
| CN101537154A (en) * | 2008-09-12 | 2009-09-23 | 罗春青 | Manufacturing method of medicament for treating ten kinds of diseases |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105395442A (en) * | 2016-01-11 | 2016-03-16 | 北京华信龙悦科技有限公司 | Natural plant long-lasting moisturizing composition and use thereof |
| CN108014059A (en) * | 2016-11-04 | 2018-05-11 | 无锡法瑞雅生物细胞科学有限公司 | A kind of skin whitening, moisturizing lotion and preparation method thereof |
| CN107669533A (en) * | 2016-12-12 | 2018-02-09 | 青岛大学 | A kind of preparation method of UV white whitening toner |
| CN106511213A (en) * | 2016-12-24 | 2017-03-22 | 张涉应 | Cosmetic containing plant extract liquid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102362842B (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101804185B (en) | Chinese medicinal compound extract for whitening and removing freckles and preparation method thereof | |
| CN104622776B (en) | A kind of cosmetic composition containing Chinese medicine extraction liquid | |
| CN102091258B (en) | Chinese medicinal composition for dispelling scars and preparation method thereof | |
| CN102028752B (en) | Chinese herbal medicine compound freckle-removing and skin-whitening composition and external preparation | |
| CN102049008A (en) | Traditional Chinese medicine composition for treating chloasma and preparation method thereof | |
| CN103041173A (en) | Traditional Chinese medicine external preparation for curing dermatitis and eczema and preparing method thereof | |
| CN105106103A (en) | Traditional Chinese medicine skincare product and preparation method thereof | |
| CN102389539B (en) | Pure traditional Chinese medicine preparation for removing speckle and beautifying, and preparation method of pulvis and particle of the preparation | |
| CN106474350A (en) | A kind of plant antibiotic compound spray and preparation method thereof | |
| CN102362842B (en) | Preparation and application of traditional Chinese medicine compound extract with whitening and freckle removing effects | |
| CN105616318A (en) | Anti-wrinkle and skin-firming herba centellae traditional Chinese medicine facial mask and preparation method therefor | |
| CN101219096A (en) | Pharmaceutical composition for removing speckle, production method and using method thereof | |
| CN109303724A (en) | A kind of novel maintenance stoste composition of releiving containing folium artemisiae argyi | |
| CN104173607B (en) | It is a kind of to treat pharmaceutical composition of chloasma and preparation method thereof | |
| CN102335231B (en) | Chinese medicinal moisturizing cream for treating chloasma as well as preparation method and application thereof | |
| CN108403932B (en) | Traditional Chinese medicine compound external preparation for treating hormone-dependent dermatitis and preparation method and application thereof | |
| CN106039022A (en) | Skin brightening and whitening traditional Chinese medicinal composition, skin brightening and whitening traditional Chinese medicinal preparation, skin brightening and whitening mask and preparation method | |
| CN110934810A (en) | Preparation method of whitening and freckle-removing cream | |
| CN101057922B (en) | Traditional Chinese medicine preparation for treating vitiligo | |
| CN104784475B (en) | A kind of Traditional Chinese medicine formula extract and its application for whitening spot-removing | |
| CN102228544B (en) | Traditional Chinese medicine composition for treating leucoderma and preparation method thereof | |
| CN101342223B (en) | Jade butterfly beauty treatment speckle removing plaster and preparation method thereof | |
| CN104068404A (en) | Health product with function of chloasma removal | |
| CN105641352A (en) | Medicinal preparation for treating facial stain and application thereof | |
| CN108403869B (en) | Traditional Chinese medicine composition for removing chloasma and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: HARBIN TEACHERS UNIV. Free format text: FORMER OWNER: GENG FANG Effective date: 20130531 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 154007 JIAMUSI, HEILONGJIANG PROVINCE TO: 150025 HARBIN, HEILONGJIANG PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20130531 Address after: Heilongjiang Normal University 150025 Harbin Limin Road Development Zone No. 1 Applicant after: Harbin Teachers' Univ. Address before: No. 39 Qianjin District Guanghua Street 154007 Heilongjiang city of Jiamusi Province Applicant before: Geng Fang |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20140930 |
|
| EXPY | Termination of patent right or utility model |