CN115943931A - Ammonia gas emission reduction method in black soldier fly bioconversion process - Google Patents
Ammonia gas emission reduction method in black soldier fly bioconversion process Download PDFInfo
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Abstract
The invention discloses an ammonia emission reduction method in a black soldier fly bioconversion process, which comprises the following steps of mixing organic waste with corn flour, adjusting the egg energy ratio to be 6-8.5 g/MJ, adding black soldier fly larvae for culturing, and regulating the ratio of the protein content of a material to the metabolic energy to realize the balance between the supply of the protein and the energy of the material and the supply and demand of the growth and metabolism of the black soldier fly and reduce the emission of ammonia. In addition, the invention finds that the combined aeration mode can further reduce the emission of ammonia gas in the transformation process of the hermetia illucens. The related technology of the invention is beneficial to ammonia gas emission reduction in the industrial breeding process of the hermetia illucens.
Description
Technical Field
The invention belongs to the technical field of biological energy conservation and environmental protection, and particularly relates to an ammonia gas emission reduction method in a black soldier fly bioconversion process.
Background
The quantity of solid organic wastes produced in China is about 40 hundred million tons every year, if the solid organic wastes cannot be timely and effectively treated, serious environmental pollution and resource waste are easily caused. In addition to environmental pollution pressure, the shortage of protein feed resources is another problem facing the breeding industry.
The Hermetia illucens (L.) larvae can convert organic wastes such as livestock and poultry excrement and kitchen waste into organisms rich in protein and fat, can be used for partially replacing soybean meal or fish meal in the animal husbandry and fishery production, has the characteristics of wide feeding property, short breeding period and large biomass, and is an effective way for solving the problems of 'waste resource utilization' and 'protein resource shortage'. The feasibility and the safety of feeding hermetia illucens by using the livestock and poultry manure of a large-scale farm are proved by a large amount of researches. In 2017, the method for producing insect protein by converting livestock and poultry manure with hermetia illucens is listed as one of 9 livestock and poultry manure resource utilization main pushing technical modes by a livestock and poultry general station in China.
The ammonia emission in the house is bred to heisui river horsefly conversion kitchen garbage in-process is very big. Therefore, reducing ammonia gas emissions during the transformation process is an important aspect of the sustainable development of hermetia illucens transformation technology. With the industrial development of the black soldier fly organic waste conversion technology, the development of an ammonia gas emission reduction technology in the black soldier fly bioconversion process is urgently needed to support the green development of the black soldier fly bioconversion industry.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
As one aspect of the invention, the invention provides an ammonia gas emission reduction method in a black soldier fly bioconversion process, which comprises the following steps of mixing organic waste with corn flour, adjusting the egg energy ratio to be 6-8.5 g/MJ, and adding black soldier fly larvae for culturing.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the culture temperature is controlled to be 35-40 ℃ in the process of culturing the hermetia illucens larvae.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: when the temperature is higher than 40 ℃, aerating by introducing air, and when the temperature is reduced to 35 ℃, immediately stopping aerating.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the organic waste comprises kitchen waste.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the egg energy ratio is adjusted to be 7.5-8.0 g/MJ.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: after mixing the organic waste and the corn flour, adjusting the water content of the mixed material to 75%.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the black soldier fly larvae are 4-day-old black soldier fly larvae; the putting amount of the black soldier fly larvae is that 80-200 black soldier fly larvae are put in every 150g of the mixture of the organic waste and the corn meal.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the dosage of the black soldier fly larvae is 100 black soldier fly larvae per 150g of the mixture of the organic waste and the corn meal.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the black soldier fly larvae are added for culture, the culture is carried out in a constant-temperature constant-humidity incubator, the initial temperature of the constant-temperature constant-humidity incubator is set to be 30 ℃, and the relative humidity is set to be 70% for culture.
The ammonia gas emission reduction method used in the black soldier fly bioconversion process comprises the following steps: the culture time is 10-11 days.
The invention has the beneficial effects that: the invention provides a method for reducing emission of ammonia gas in a process of converting hermetia illucens into organic waste. By regulating and controlling the ratio of the protein content of the material to the metabolic energy, the balance between the supply of the protein and the energy of the material and the supply and demand of the growth and metabolism of the hermetia illucens is realized, and the emission of ammonia gas is reduced. In addition, the invention finds that the combined aeration mode can further reduce the emission of ammonia gas in the transformation process of the hermetia illucens. The related technology of the invention is beneficial to ammonia gas emission reduction in the industrial breeding process of the hermetia illucens.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
The kitchen waste was collected, mixed well and divided into equal portions for use in the following examples.
The nutritional components of the kitchen waste and the corn flour used in the invention are as follows:
example 1:
the method comprises the steps of adjusting the egg energy ratio (namely the ratio of protein content to metabolic energy) to be 9.03g/MJ and the water content of a material to be 75% by mixing 150g (calculated by dry matters) of kitchen waste and corn meal, fully stirring and uniformly mixing, directly putting 100 heads of 4-day-old hermetia illucens larvae into the surface layer of the material to perform material conversion, putting the material into an incubator to perform culture, setting the relative humidity to be 70% and the initial temperature to be 30 ℃, and continuously culturing for 10 days.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 2:
and (3) adjusting the egg energy ratio to be 8.5g/MJ and the water content of the materials to be 75% by mixing 150g (calculated by dry matters) of the kitchen waste and corn flour, and fully stirring and uniformly mixing. Directly putting 100 black soldier fly larvae of 4 days old into the surface layer of the material for material conversion, putting into an incubator for cultivation, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously cultivating for 10 days.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 3:
and (3) adjusting the egg energy ratio to 7.5g/MJ and the water content of the materials to 75% by mixing 150g (calculated by dry matters) of the kitchen waste and corn flour, and fully stirring and uniformly mixing. Directly putting 100 black soldier fly larvae of 4 days old into the surface layer of the material for material conversion, putting into an incubator for cultivation, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously cultivating for 10 days.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 4:
adjusting the egg energy ratio of the kitchen waste and corn flour mixed material to be 6.0g/MJ, adjusting the water content of the material to be 75%, and fully stirring and uniformly mixing, wherein the total amount of the kitchen waste and corn flour mixed material is 150g (calculated by dry matter). Directly putting 100-head 4-day-old hermetia illucens larvae into the surface layer of the material for material conversion, putting the material into an incubator for culture, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously culturing for 10 days.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 5:
and (3) adjusting the egg energy ratio to 5.0g/MJ and the water content of the materials to 75% by mixing 150g (calculated by dry matters) of the kitchen waste and corn flour, and fully stirring and uniformly mixing. Directly putting 100-head 4-day-old hermetia illucens larvae into the surface layer of the material for material conversion, putting the material into an incubator for culture, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously culturing for 1O day.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 6:
and (3) adjusting the egg energy ratio to be 4.0g/MJ and the water content of the materials to be 75% by mixing 150g (calculated by dry matters) of the kitchen waste and corn flour, and fully stirring and uniformly mixing. Directly putting 100-head 4-day-old hermetia illucens larvae into the surface layer of the material for material conversion, putting the material into an incubator for culture, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously culturing for 1O day.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
Example 7:
and (3) adjusting the egg energy ratio to 7.5g/MJ and the water content of the materials to 75% by mixing 150g (calculated by dry matters) of the kitchen waste and corn flour, and fully stirring and uniformly mixing. Directly putting 100-head 4-day-old hermetia illucens larvae into the surface layer of the material for material conversion, putting the material into an incubator for culture, setting the relative humidity to be 70%, setting the initial temperature to be 30 ℃, and continuously culturing for 1O day.
As the temperature of the materials rises along with the prolonging of the culture time, the temperature of the materials is detected in the conversion process, when the temperature of the materials is higher than 40 ℃, air is immediately introduced for aeration, and when the temperature is reduced to 35 ℃, the aeration is immediately stopped.
And collecting and detecting ammonia gas discharged in the culture process by adopting a dilute sulfuric acid in-situ absorption method every day in the conversion process.
TABLE 1 influence of different egg energies on ammonia gas emission during black soldier fly conversion compared to the material
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. An ammonia gas emission reduction method in a hermetia illucens biotransformation process is characterized in that: the method comprises the following steps of mixing organic waste with corn flour, adjusting the egg energy ratio to be 6-8.5 g/MJ, and adding hermetia illucens larvae for culturing.
2. The method for reducing ammonia gas emission from a black soldier fly bioconversion process of claim 1, wherein: the culture temperature is controlled to be 35-40 ℃ in the process of culturing the hermetia illucens larvae.
3. The method for reducing ammonia gas emission from a black soldier fly bioconversion process of claim 2, wherein: when the temperature is higher than 40 ℃, air is introduced for aeration, and when the temperature is reduced to 35 ℃, the aeration is stopped.
4. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: the organic waste comprises kitchen waste.
5. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: the egg energy ratio is adjusted to be 7.5-8.0 g/MJ.
6. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: after mixing the organic waste with the corn flour, adjusting the water content of the mixed material to 75%.
7. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: the black soldier fly larvae are 4-day-old black soldier fly larvae; the putting amount of the black soldier fly larvae is that 80-200 black soldier fly larvae are put in every 150g of the mixture of the organic waste and the corn meal.
8. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: the dosage of the black soldier fly larvae is 100 black soldier fly larvae per 150g of the mixture of the organic waste and the corn meal.
9. The method for reducing ammonia gas emission from a black soldier fly bioconversion process according to any one of claims 1 to 3, wherein: the black soldier fly larvae are added for culture, the culture is carried out in a constant-temperature constant-humidity incubator, the initial temperature of the constant-temperature constant-humidity incubator is set to be 30 ℃, and the relative humidity is set to be 70% for culture.
10. The method of claim 9 for reducing ammonia gas emissions from the black soldier fly bioconversion process, wherein: the culture time is 10-11 days.
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