CN115927443A - Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees - Google Patents
Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees Download PDFInfo
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- CN115927443A CN115927443A CN202310044603.0A CN202310044603A CN115927443A CN 115927443 A CN115927443 A CN 115927443A CN 202310044603 A CN202310044603 A CN 202310044603A CN 115927443 A CN115927443 A CN 115927443A
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- hexenol
- gene
- tea
- drought resistance
- csadh
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- UFLHIIWVXFIJGU-ARJAWSKDSA-N (Z)-hex-3-en-1-ol Chemical compound CC\C=C/CCO UFLHIIWVXFIJGU-ARJAWSKDSA-N 0.000 title claims abstract description 46
- UFLHIIWVXFIJGU-UHFFFAOYSA-N hex-3-en-1-ol Natural products CCC=CCCO UFLHIIWVXFIJGU-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 235000009024 Ceanothus sanguineus Nutrition 0.000 title claims abstract description 35
- 240000003553 Leptospermum scoparium Species 0.000 title claims abstract description 35
- 235000015459 Lycium barbarum Nutrition 0.000 title claims abstract description 35
- 230000033228 biological regulation Effects 0.000 title abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 29
- 244000269722 Thea sinensis Species 0.000 claims abstract description 20
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 11
- 230000014509 gene expression Effects 0.000 claims abstract description 5
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 8
- 235000006468 Thea sinensis Nutrition 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 2
- 230000002018 overexpression Effects 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 9
- 241000208125 Nicotiana Species 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 5
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 229930014626 natural product Natural products 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 4
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 3
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 3
- 230000030279 gene silencing Effects 0.000 description 3
- 238000012226 gene silencing method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- GXANMBISFKBPEX-ARJAWSKDSA-N cis-3-hexenal Chemical compound CC\C=C/CC=O GXANMBISFKBPEX-ARJAWSKDSA-N 0.000 description 2
- 230000008641 drought stress Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- GXANMBISFKBPEX-UHFFFAOYSA-N hex-3c-enal Natural products CCC=CCC=O GXANMBISFKBPEX-UHFFFAOYSA-N 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Images
Abstract
The invention discloses a regulation and control method of (Z) -3-hexenol and application thereof in tea tree drought resistance, wherein the (Z) -3-hexenol with the working concentration of 0.01-0.5 mu g/L is added on tea seedlings for slow release and drought treatment, and the drought resistance of tea trees can be effectively improved. The CsADH gene is overexpressed in the tobacco, the (Z) -3-hexenol content in the tobacco is increased, and the drought resistance of tobacco plants is improved; the tea tree silences a CsADH gene, reduces the (Z) -3-hexenol content in the tea tree, and reduces the drought resistance of the tea tree. The synthesis of (Z) -3-hexenol can be effectively regulated and controlled by regulating the expression quantity of the CsADH gene, and the drought resistance of tea trees can be regulated and controlled. Only one component is needed to act on the (Z) -3-hexenol independently, the use concentration is low, the acting force is strong, the cost is low, and the (Z) -3-hexenol is a natural product in the nature, is volatilized and naturally degraded, and is non-toxic and harmless to the environment.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees.
Background
Tea tree (Camellia sinensis) belongs to perennial evergreen woody plants, is warm and wet, but has weak drought tolerance. As with other crops, drought has become one of the major abiotic stresses that restrict tea production, and this problem tends to be severe with global warming and increasingly scarce water resources, and it has been reported that drought stress can reduce tea yield by 14-33% and increase the mortality of tea plants by 6-19%, with serious economic losses to the tea industry.
The current drought resisting technology of the tea garden mainly comprises the following steps: timely irrigation, tea row coverage, reduction of pruning, timely fertilization, establishment of shading facilities and the like. Volatile substances have many physiological functions, including enhancing stress tolerance in plants. (Z) -3-hexenol is synthesized from (Z) -3-hexenal in tea tree under the catalysis of Alcohol Dehydrogenase (ADH), and the gene CsADH for ADH is found in tea tree. The (Z) -3-hexenol has very important function in the direct defense of tea trees and is mainly used for defending potential pests. The research for improving the drought resistance of tea trees is rare.
Based on the analysis, a method for controlling drought resistance of tea trees by regulating volatile substances is urgently needed in the industry at present.
Disclosure of Invention
In view of the defects, the invention provides a method for controlling (Z) -3-hexenol to deal with drought resistance of tea trees, so that the drought resistance of tea seedlings is improved, and a new mode is found for the traditional drought resistance technology.
The invention is realized by the following technical means:
the invention discloses application of (Z) -3-hexenol in drought resistance of tea trees.
Furthermore, the working concentration of the (Z) -3-hexenol is 0.01-0.5 mu g/L.
Furthermore, (Z) -3-hexenol with the working concentration of 0.01-0.5 mu g/L is added on the tea seedlings for slow release, the treatment is carried out for 12 hours, and then the drought treatment is carried out, so that the drought resistance of the tea seedlings can be improved.
The invention also discloses a gene capable of improving the content of (Z) -3-hexenol in tobacco by overexpression, wherein: the gene is CsADH gene.
Further, the CsADH gene is overexpressed, the content of (Z) -3-hexenol is increased, and the drought resistance of plants is improved.
The invention also discloses a gene capable of reducing the content of (Z) -3-hexenol in tea trees by silent expression, wherein: the gene is CsADH gene.
Furthermore, the invention also discloses application of the gene in construction of a tea plant line with low (Z) -3-hexenol content.
Furthermore, the CsADH gene is silenced in the tea tree, the (Z) -3-hexenol content in the tea tree is reduced, and the drought resistance of the tea tree is reduced.
The invention has the beneficial effects that:
1. according to the invention, the (Z) -3-hexenol is adopted to pretreat the tea trees, so that the drought resistance of the tea trees can be effectively improved.
2. The synthesis of (Z) -3-hexenol can be effectively regulated and controlled by regulating the expression quantity of the CsADH gene, and the drought resistance of tea trees can be regulated and controlled.
3. Only one component is needed to act on the (Z) -3-hexenol independently, the use concentration is low, the acting force is strong, the cost is low, and the (Z) -3-hexenol is a natural product in the nature, the fragrance is volatilized and naturally degraded, and the (Z) -3-hexenol is non-toxic and harmless to the environment.
Drawings
FIG. 1a shows the phenotype of the plants and the water content of the leaves after the tea seedlings were pre-treated with volatile (Z) -3-hexenol and then subjected to drought treatment; 1b represents the plant phenotype and leaf water content after the CsADH gene is over-expressed in tobacco and the drought stress is generated.
FIG. 2a shows the (Z) -3-hexenol synthesis pathway; 2b shows a schematic representation of the gene silencing process; 2c, e shows the (Z) -3-hexenol content in the leaf after the CsADH gene is silenced; 2d represents the relative expression level of CsADH after gene silencing; 2f represents the phenotype of the drought-treated plant after CsADH gene silencing; 2g and h represent the leaf angle and the leaf water content of the drought-treated plant after the CsADH gene is silenced.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
(Z) -3-hexenol regulation and control method and application thereof in drought resistance of tea trees
1) Adding (Z) -3-hexenol with mother liquor concentration of 1mM on absorbent cotton, placing the absorbent cotton and tea seedlings in a 5L closed container for treatment for 12h, naturally volatilizing fragrance (final concentration of 0.1 mug/L, substance mass/air volume), treating once every 6 days, adding an equal amount of solvent into a control group, and then carrying out drought treatment for 12 days.
2) The CsADH gene is overexpressed in the tobacco, the (Z) -3-hexenol content in the tobacco is increased, and the drought resistance of tobacco plants is improved; the tea tree silences a CsADH gene, reduces the (Z) -3-hexenol content in the tea tree, and reduces the drought resistance of the tea tree.
The results show that:
1) The (Z) -3-hexenol is added for treatment, so that the drought resistance of the tea seedlings can be obviously improved, and after the tea seedlings are subjected to drought for 12 days, the water content of leaves of a treatment group is obviously higher than that of leaves of a control group (figure 1 a).
2) After the CsADH is over-expressed in the tobacco, the drought resistance is also obviously improved, and the tobacco leaves show higher water content (figure 1 b).
3) The CsADH is silenced in tea trees, and the content of (Z) -3-hexenol is reduced, so that the result shows that the drought resistance of tea seedlings silenced by CsAHD is obviously reduced. The drought resistance of (Z) -3-hexenol was further demonstrated (FIG. 2).
In conclusion, the (Z) -3-hexenol is a natural product, can be degraded in nature, is non-toxic and harmless to the environment, and has extremely high application potential in regulating and controlling the drought resistance of tea trees by regulating and controlling the synthesis of the (Z) -3-hexenol.
Claims (8)
1. The application of (Z) -3-hexenol in drought resistance of tea trees.
2. The use according to claim 1, wherein:
the working concentration of the (Z) -3-hexenol is 0.01-0.5 mu g/L.
3. The use according to claim 1 or 2, comprising:
adding (Z) -3-hexenol with the working concentration of 0.01-0.5 mu g/L on the tea seedlings for slow release, treating for 12 hours, and then carrying out drought treatment to improve the drought resistance of the tea seedlings.
4. An over-expression gene capable of increasing the content of (Z) -3-hexenol in tobacco, wherein:
the gene is CsADH gene.
5. The gene of claim 4, wherein:
the CsADH gene is over-expressed, the content of (Z) -3-hexenol is improved, and the drought resistance of plants is improved.
6. A gene whose silent expression can reduce the content of (Z) -3-hexenol in tea tree, wherein:
the gene is CsADH gene.
7. Use of the gene of claim 6 in the construction of a tea plant line with low (Z) -3-hexenol content.
8. The use of claim 7, comprising:
the CsADH gene is silenced in the tea tree, the (Z) -3-hexenol content in the tea tree is reduced, and the drought resistance of the tea tree is reduced.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310044603.0A CN115927443A (en) | 2023-01-30 | 2023-01-30 | Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees |
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| CN202310044603.0A CN115927443A (en) | 2023-01-30 | 2023-01-30 | Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees |
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| CN115927443A true CN115927443A (en) | 2023-04-07 |
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| CN202310044603.0A Pending CN115927443A (en) | 2023-01-30 | 2023-01-30 | Regulation and control of (Z) -3-hexenol and application thereof in drought resistance of tea trees |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224946A (en) * | 2013-05-14 | 2013-07-31 | 浙江大学 | Tea tree beta-glucosaccharase gene bGlu and application thereof |
| CN108070576A (en) * | 2018-02-05 | 2018-05-25 | 安徽农业大学 | A kind of tea tree glycosyl transferase mutant and its application in plant insect defence |
| CN109287629A (en) * | 2018-11-07 | 2019-02-01 | 华中农业大学 | The new application of cis- 3- hexenol and adverse-resistant agent including cis- 3- hexenol |
| CN110475471A (en) * | 2017-01-30 | 2019-11-19 | 科沃施种子欧洲股份两合公司 | Show the rotaring gene corn plant of increased yield and drought tolerance |
-
2023
- 2023-01-30 CN CN202310044603.0A patent/CN115927443A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224946A (en) * | 2013-05-14 | 2013-07-31 | 浙江大学 | Tea tree beta-glucosaccharase gene bGlu and application thereof |
| CN110475471A (en) * | 2017-01-30 | 2019-11-19 | 科沃施种子欧洲股份两合公司 | Show the rotaring gene corn plant of increased yield and drought tolerance |
| CN108070576A (en) * | 2018-02-05 | 2018-05-25 | 安徽农业大学 | A kind of tea tree glycosyl transferase mutant and its application in plant insect defence |
| CN109287629A (en) * | 2018-11-07 | 2019-02-01 | 华中农业大学 | The new application of cis- 3- hexenol and adverse-resistant agent including cis- 3- hexenol |
Non-Patent Citations (2)
| Title |
|---|
| SHENGRUI LIU等: "Identi fi cation and Functional Analysis of Two Alcohol Dehydrogenase Genes Involved in Catalyzing the Reduction of ( Z ) ‑ 3-Hexenal into ( Z ) ‑ 3-Hexenol in Tea Plants ( Camellia sinensis )", J. AGRIC. FOOD CHEM, vol. 70, no. 6, pages 1830 - 1839 * |
| 全文选;丁贵杰;: "干旱胁迫下马尾松幼苗针叶挥发性物质与内源激素的变化", 林业科学, no. 04, pages 49 - 55 * |
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