CN115918541B - Macleaya cordata plant tissue culture tank - Google Patents
Macleaya cordata plant tissue culture tank Download PDFInfo
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- CN115918541B CN115918541B CN202211693614.3A CN202211693614A CN115918541B CN 115918541 B CN115918541 B CN 115918541B CN 202211693614 A CN202211693614 A CN 202211693614A CN 115918541 B CN115918541 B CN 115918541B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention provides a macleaya cordata plant tissue culture tank which comprises a bottle body, wherein a base is embedded in the bottom of the bottle body, the bottom of the bottle body is tightly attached to the upper surface of the base, a cultivation component for planting macleaya cordata plant tissues is arranged in the middle of the base, a nutrient solution adding component is fixed above the inside of the bottle body, and a sealing component is fixed at the top of the bottle body.
Description
Technical Field
The invention relates to the technical field of plant assembly culture equipment, in particular to a macleaya cordata plant tissue culture tank.
Background
The macleaya cordata contains various alkaloids, is a plant with medicinal value, and because the active and effective components of the macleaya cordata are natural substances, the macleaya cordata is easy to degrade and has no residue after being used, so that the macleaya cordata has been increasingly focused on research and application as feed additives, veterinary drugs, medicines and cosmetics, and human beings utilize the historical long-range of the macleaya cordata, and when the collection amount exceeds the regeneration capability of natural resources of the macleaya cordata, the macleaya cordata is inevitably endangered or even extinct. Therefore, the propagation efficiency of the macleaya cordata is improved, and the macleaya cordata is beneficial to saving and protecting the plant germplasm resources of the macleaya cordata. The macleaya cordata is sometimes propagated by utilizing an assembly culture technology, the macleaya cordata is propagated by utilizing a tissue culture technology, and the method has the advantages of maintaining excellent properties, being short in production period, high in propagation efficiency, free from the influence of regions, seasons, harmful organisms and the like, is an efficient and feasible plant propagation mode, and generally uses a culture tank to place the macleaya cordata in the culture tank for propagation, and in the prior art, a plurality of culture tanks and culture bottles exist;
through retrieving, like the novel plant tissue culture bottle of patent number CN201510942100.0 among the prior art, the bottle cover is split type structure with the jar, and the size of jar is the pipe diameter size promptly, when needs operation, experimental record take a picture and the seedling transplantation, with the bottle cover with jar separation can, have the direct-view operation of being convenient for, be difficult for damaging root, stem, the leaf advantage of plant.
However, when the macleaya cordata tissue is taken out from the bottle cylinder after the growth of the macleaya cordata tissue in the bottle holder is completed, the cultured macleaya cordata is taken out from the bottle cylinder after the bottle cover is separated by pulling and lifting, the root and the stem of the macleaya cordata are easily damaged in the taking-out process, the taking-out speed is low, and the operation is inconvenient.
Disclosure of Invention
In order to solve the technical problems, the invention provides a macleaya cordata plant tissue culture tank for solving the problems described in the background art.
The invention discloses a macleaya cordata plant tissue culture tank which is prepared by the following specific technical means: the patent refers to the field of 'cultivation in general'.
As a further scheme of the invention: the cultivation component consists of a cultivation basin, a bottom plate, a separation plate and a connecting column;
specifically, the top of base is fixed with cultivates the basin, cultivate the inside below embedding of basin and have the bottom plate, the outside of bottom plate is fixed with the separator plate, the bottom of bottom plate is fixed with the spliced pole.
As a further scheme of the invention: the upper surface opening of cultivateing the basin is provided with a hidden groove, bottom plate and separator just in time imbeds in hiding the groove, the spliced pole of bottom plate below stretches out from the middle part below of base.
As a further scheme of the invention: the adding component consists of a collecting disc, a guide frame, a rotating disc, blades and a spray head;
specifically, the inside top of body is fixed with the collection dish, the upper surface middle part of collection dish is fixed with the guide disc, the upper surface fixed of guide disc has the leading truck, the middle part embedding of guide disc has the rolling disc, the upper surface fixed of rolling disc has the blade, the bottom of rolling disc is fixed with the shower nozzle.
As a further scheme of the invention: the guide frame in the guide disc is arranged in an arc shape with a clockwise opening from inside to outside, the blades in the rotating disc are arranged in an arc shape with a clockwise opening from inside to outside, and the upper surface of the guide disc and the upper surface of the rotating disc are both positioned on the same horizontal plane.
As a further scheme of the invention: the inside vertical trompil of rolling disc is provided with the feed liquor passageway, the feed liquor passageway sets up between adjacent blade, the bottom of feed liquor passageway switches on with the inside of shower nozzle mutually, the middle part of rolling disc and the middle part of rolling disc all are in on the same vertical horizontal plane.
As a further scheme of the invention: the sealing component consists of a top plate, a liquid discharge cylinder, an outer frame, a middle plate, a sealing plate, an inner ring, a connecting column and a spring;
specifically, the inside top of body is fixed with the roof, the inner wall below embedding of roof has a flowing back section of thick bamboo, the inside embedding of roof has outer frame, the middle part of outer frame is fixed with the medium plate, the bottom of outer frame is fixed with the closing plate, the inside top embedding of roof has the inner loop, the inside embedding of inner loop has the spliced pole, and novel spliced pole's outside nestification has the spring.
As a further scheme of the invention: the inner opening of the top plate is provided with a ring groove, the liquid draining cylinder is communicated with the inner part of the ring groove, and the inner wall of the sealing plate is tightly clung to the inner side of the ring groove.
As a further scheme of the invention: the top of the ring groove is fixedly provided with a sealing ring, the top of the outer frame is tightly attached to the bottom end of the sealing ring, the outer frame vertically ascends and descends in the ring groove, and the distance between the outer wall of the outer frame and the ring groove is 0.5-1cm.
As a further scheme of the invention: the baffle which is consistent with the aperture of the liquid discharge cylinder is fixed below the bottom end of the sealing plate through a connecting rod, and the baffle is just embedded and arranged below the inner part of the liquid discharge cylinder.
The invention provides a macleaya cordata plant tissue culture tank which has the following beneficial effects:
1. after the cultivation of macleaya cordata plant tissue is completed, the bottle body and the base can be separated, the connecting column is pressed in the follow-up accessible mode, the connecting column pushes the bottom plate and the separating plate arranged at the bottom end of the cultivation basin to move upwards, the bottom plate and the separating plate push the macleaya cordata plant tissue to lift up from the cultivation basin, the separation between the cultivation basin and the macleaya cordata plant tissue is realized, the rootstock of the macleaya cordata plant tissue is not easy to damage in the separation process, the protection effect on the macleaya cordata plant tissue is good, and the operation is simpler and more convenient.
2. When the nutrient solution is added to the middle plate, the middle plate moves downwards due to the fact that the gravity nutrient solution pushes the middle plate to move downwards through the outer frame pushing baffle plate, the middle plate stretches out from the lower portion of the liquid discharge cylinder, after the nutrient solution enters the annular groove in the top plate along the middle plate, the nutrient solution is discharged along the liquid discharge cylinder, when the nutrient solution does not exist, the spring pulls the middle plate to move upwards through the connecting column to restore to original position, the outer frame is tightly attached to the sealing ring above the annular groove, the baffle plate is embedded into the liquid discharge cylinder, double sealing of the top of the bottle body is further achieved, external air is prevented from entering the device, and sealing performance is good.
3. Nutrient solution is discharged along the collecting tray in the leading truck of leading truck when the landing, later gets into the upper surface striking rolling disc upper surface of rolling disc for the rolling disc is rotatory, later the nutrient solution can get into the cavity passageway of rolling disc and discharge from the shower nozzle, realizes spraying the replenishment nutrient solution to macleaya cordata plant tissue while rotatory, reduces and sprays the dead angle, and the nutrient solution adds more evenly.
Drawings
FIG. 1 is a schematic cross-sectional front view of an overall structure of the present invention;
FIG. 2 is a schematic top view of a partial structure of a base plate according to the present invention;
FIG. 3 is a schematic top view of a partial structure of a guide disc and a rotating disc according to the present invention;
FIG. 4 is a schematic top view of a partial structure of a guide disc according to the present invention;
FIG. 5 is a schematic top view of a partial structure of a rotary disk according to the present invention;
FIG. 6 is a schematic top view showing a partial structure of the culture pot according to the present invention;
FIG. 7 is an enlarged view of the structure of FIG. 1A according to the present invention.
Legend description:
the bottle body 1, the base 2, the culture basin 201, the bottom plate 202, the separation plate 203, the connecting column 204, the collecting tray 3, the guide tray 301, the guide frame 302, the rotating tray 303, the blade 304, the spray head 305, the top plate 4, the liquid discharge tube 401, the outer frame 402, the middle plate 403, the sealing plate 404, the inner ring 405, the connecting column 406 and the spring 407.
Detailed Description
Embodiment 1 please refer to fig. 1-7, a macleaya cordata plant tissue culture tank is characterized by comprising a bottle body 1, wherein a base 2 is embedded in the bottom of the bottle body 1, the bottom of the bottle body 1 is tightly attached to the upper surface of the base 2, a cultivation component for planting macleaya cordata plant tissues is arranged in the middle of the base 2, a nutrient solution adding component is fixed above the inside of the bottle body 1, and a sealing component is fixed at the top of the bottle body 1.
The cultivation component consists of a cultivation basin 201, a bottom plate 202, a separation plate 203 and a connecting column 204;
a culture basin 201 is fixed at the top of the base 2, a bottom plate 202 is embedded below the inner part of the culture basin 201, a separation plate 203 is fixed at the outer side of the bottom plate 202, and a connecting column 204 is fixed at the bottom of the bottom plate 202;
the upper surface opening of the culture basin 201 is provided with a hidden groove, the bottom plate 202 and the separating plate 203 are just embedded in the hidden groove, and the connecting column 204 below the bottom plate 202 extends out from the lower part of the middle part of the base 2;
the bottle body 1 is connected with the base 2 through bolts, so that the connection between the bottle body 1 and the base 2 is more stable;
the macleaya cordata plant tissue is split with the bottle body 1 and the base 2 after the culture is completed, the connecting column 204 is pressed, so that the connecting column 204 pushes the bottom plate 202 and the separating plate 203 arranged at the bottom end of the culture basin 201 to move upwards, the bottom plate 202 and the separating plate 203 push the macleaya cordata plant tissue to lift up from the culture basin, the separation of the culture basin 201 and the macleaya cordata plant tissue is realized, the rootstock of the macleaya cordata plant tissue is not easy to damage in the separation process, and the protective effect on the macleaya cordata plant tissue is good.
Embodiment 2 referring to fig. 1 to 5, embodiment 2 is different from embodiment 1 in that the adding assembly is composed of a collecting tray 3, a guide tray 301, a guide frame 302, a rotating tray 303, vanes 304 and a spray head 305;
a collecting tray 3 is fixed above the inside of the bottle body 1, a guide tray 301 is fixed in the middle of the upper surface of the collecting tray 3, a guide frame 302 is fixed on the upper surface of the guide tray 301, a rotating tray 303 is embedded in the middle of the guide tray 301, blades 304 are fixed on the upper surface of the rotating tray 303, and a spray head 305 is fixed at the bottom of the rotating tray 303;
the guide frame 302 in the guide plate 301 is arranged in an arc shape with a clockwise opening from inside to outside, the blades 304 in the rotating plate 303 are arranged in an arc shape with a clockwise opening from inside to outside, and the upper surface of the guide plate 301 and the upper surface of the rotating plate 303 are both positioned on the same horizontal plane;
after being discharged from the liquid discharge cylinder 401, the nutrient solution slides along the collecting disc 3 and enters the guide disc 301, and due to the fall between the upper part and the lower part, the nutrient solution can generate gravity, is discharged along the guide frame 302 and enters the upper surface of the rotating disc 303 to impact the blades 304 on the upper surface of the rotating disc, so that the rotating disc 303 rotates clockwise;
the vertical opening in the rotating disc 303 is provided with a liquid inlet channel, the liquid inlet channel is arranged between adjacent blades 304, the bottom of the liquid inlet channel is communicated with the inside of the spray head 305, and the middle part of the rotating disc 303 are both positioned on the same vertical horizontal plane;
and then, the nutrient solution can enter a hollow channel of the rotating disc 303 and is discharged from the spray head, and the nutrient solution is just sprayed on the macleaya cordata plant tissues planted in the culture basin 201, so that the macleaya cordata plant tissues are sprayed and supplemented with the nutrient solution while rotating, and the nutrient solution is added more uniformly.
Embodiment 3 referring to fig. 1-7, embodiment 3 is different from embodiment 2 in that the sealing assembly is composed of a top plate 4, a liquid discharge cylinder 401, an outer frame 402, a middle plate 403, a sealing plate 404, an inner ring 405, a connecting column 406 and a spring 407;
a top plate 4 is fixed above the inside of the bottle body 1, a liquid discharge cylinder 401 is embedded below the inner wall of the top plate 4, an outer frame 402 is embedded inside the top plate 4, a middle plate 403 is fixed in the middle of the outer frame 402, a sealing plate 404 is fixed at the bottom of the outer frame 402, an inner ring 405 is embedded above the inside of the top plate 4, a connecting column 406 is embedded inside the inner ring 405, and a spring 407 is embedded outside the novel connecting column 406;
the inner opening of the top plate 4 is provided with a ring groove, the liquid discharge cylinder 401 is communicated with the inner part of the ring groove, and the inner wall of the sealing plate 404 is tightly clung to the inner side of the ring groove;
a sealing ring is fixed at the top of the ring groove, the top of the outer frame 402 is tightly attached to the bottom end of the sealing ring, the outer frame 402 vertically ascends and descends in the ring groove, and the distance between the outer wall of the outer frame 402 and the ring groove is 0.5-1cm;
the spacing is such that nutrient solution slides off the outer wall of the middle plate 403;
a baffle with the same aperture size as the liquid discharge cylinder 401 is fixed below the bottom end of the sealing plate 404 through a connecting rod, and the baffle is just embedded and arranged below the inside of the liquid discharge cylinder 401;
when the nutrient solution is added to the middle plate 403 of the bottle body 1, the middle plate 403 is pushed to move downwards by gravity, the middle plate 403 pushes the baffle plate to move downwards through the outer frame 402 to extend out from the lower part of the liquid discharge cylinder 401, the connecting column 406 is pulled to squeeze the spring 407 in the inner ring 405, and then the nutrient solution enters the annular groove in the top plate 4 along the middle plate 403 and is discharged along the liquid discharge cylinder 401;
when no nutrient solution exists, the spring 407 pulls the middle plate 403 to move upwards to restore to the original position through the connecting column 406, so that the sealing ring and the baffle plate of the outer frame 402, which are tightly attached to the upper part of the ring groove, are embedded into the liquid discharge cylinder 401, and further double sealing of the top of the bottle body 1 is realized, external air is prevented from entering the device, and the sealing performance is good;
wherein the middle plate 403, the collecting tray 3, the guide tray 301, the guide frame 302, the rotating tray 303 and the blades 304 are all made of a highly transparent material.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that modifications and improvements can be made by those skilled in the art without departing from the spirit of the present invention, and these should also be considered as the scope of the present invention, which does not affect the effect of the implementation of the present invention and the utility of the patent.
Claims (2)
1. The macleaya cordata plant tissue culture tank is characterized by comprising a bottle body, wherein a base is embedded in the bottom of the bottle body, the bottom of the bottle body is tightly attached to the upper surface of the base, a cultivation component for planting macleaya cordata plant tissues is arranged in the middle of the base, a nutrient solution adding component is fixed above the inside of the bottle body, and a sealing component is fixed at the top of the bottle body;
the cultivation component consists of a cultivation basin, a bottom plate, a separation plate and a connecting column;
the top of the base is fixedly provided with a culture basin, a bottom plate is embedded below the interior of the culture basin, a separation plate is fixed on the outer side of the bottom plate, and a connecting column is fixed at the bottom of the bottom plate;
the upper surface opening of the culture basin is provided with a hiding groove, the bottom plate and the separating plate are just embedded in the hiding groove, and the connecting column below the bottom plate extends out from the lower part of the middle part of the base;
the adding component consists of a collecting disc, a guide frame, a rotating disc, blades and a spray head;
a collecting disc is fixed above the inside of the bottle body, a guide disc is fixed in the middle of the upper surface of the collecting disc, a guide frame is fixed on the upper surface of the guide disc, a rotating disc is embedded in the middle of the guide disc, blades are fixed on the upper surface of the rotating disc, and a spray head is fixed at the bottom of the rotating disc;
the guide frame in the guide disc is arranged in an arc shape with a clockwise opening from inside to outside, the blades in the rotating disc are arranged in an arc shape with a clockwise opening from inside to outside, and the upper surface of the guide disc and the upper surface of the rotating disc are both positioned on the same horizontal plane;
the sealing component consists of a top plate, a liquid discharge cylinder, an outer frame, a middle plate, a sealing plate, an inner ring, a connecting column and a spring;
a top plate is fixed above the inner part of the bottle body, a liquid draining cylinder is embedded below the inner wall of the top plate, an outer frame is embedded inside the top plate, a middle plate is fixed in the middle of the outer frame, a sealing plate is fixed at the bottom of the outer frame, an inner ring is embedded above the inner part of the top plate, a connecting column is embedded inside the inner ring, and a spring is embedded outside the connecting column;
the inner opening of the top plate is provided with a ring groove, the liquid discharge cylinder is communicated with the inner part of the ring groove, and the inner wall of the sealing plate is tightly clung to the inner side of the ring groove;
the top of the ring groove is fixedly provided with a sealing ring, the top of the outer frame is tightly attached to the bottom end of the sealing ring, the outer frame vertically ascends and descends in the ring groove, and the distance between the outer wall of the outer frame and the ring groove is 0.5-1cm;
the baffle which is consistent with the aperture of the liquid discharge cylinder is fixed below the bottom end of the sealing plate through a connecting rod, and the baffle is just embedded and arranged below the inner part of the liquid discharge cylinder.
2. The macleaya cordata plant tissue culture tank of claim 1, wherein the inner vertical opening of the rotating disc is provided with a liquid inlet channel, the liquid inlet channel is arranged between adjacent blades, the bottom of the liquid inlet channel is communicated with the inner part of the spray head, and the middle part of the rotating disc and the middle part of the culture basin are both positioned on the same vertical horizontal plane.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211693614.3A CN115918541B (en) | 2022-12-28 | 2022-12-28 | Macleaya cordata plant tissue culture tank |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211693614.3A CN115918541B (en) | 2022-12-28 | 2022-12-28 | Macleaya cordata plant tissue culture tank |
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| CN115918541A CN115918541A (en) | 2023-04-07 |
| CN115918541B true CN115918541B (en) | 2023-08-04 |
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|---|---|
| CN115918541A (en) | 2023-04-07 |
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