CN115918541A - Macleaya cordata plant tissue culture jar - Google Patents

Macleaya cordata plant tissue culture jar Download PDF

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Publication number
CN115918541A
CN115918541A CN202211693614.3A CN202211693614A CN115918541A CN 115918541 A CN115918541 A CN 115918541A CN 202211693614 A CN202211693614 A CN 202211693614A CN 115918541 A CN115918541 A CN 115918541A
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China
Prior art keywords
plate
fixed
plant tissue
macleaya cordata
tissue culture
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CN202211693614.3A
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Chinese (zh)
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CN115918541B (en
Inventor
谭兴雅
田儒熹
邱天源
张开锋
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Guizhou Bosheng Agricultural Development Co ltd
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Guizhou Bosheng Agricultural Development Co ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention provides a macleaya cordata plant tissue culture tank, which comprises a bottle body, wherein a base is embedded in the bottom of the bottle body, the bottom of the bottle body is tightly attached to the upper surface of the base, a culture assembly for planting macleaya cordata plant tissues is arranged in the middle of the base, a nutrient solution adding assembly is fixed above the inner part of the bottle body, and a sealing assembly is fixed at the top of the bottle body.

Description

Macleaya cordata plant tissue culture jar
Technical Field
The invention relates to the technical field of plant assembly culture equipment, in particular to a macleaya cordata plant tissue culture tank.
Background
The macleaya cordata contains various alkaloids, is a plant with medicinal value, and because the active effective components of the macleaya cordata are natural substances and are easy to degrade and have no residue after being used, the macleaya cordata has attracted more and more attention for research and application as feed additives, veterinary drugs, medicines and cosmetics, and the mankind has long history of using the macleaya cordata, and when the collection amount exceeds the regeneration capacity of natural resources of the macleaya cordata, the macleaya cordata species is endangered or even killed. Therefore, the propagation efficiency of the macleaya cordata is improved, and the germplasm resources of the macleaya cordata are saved and protected. Macleaya cordata is sometimes bred by an assembly culture technology, and macleaya cordata is bred by a tissue culture technology, so that the efficient and feasible plant breeding mode has the advantages of maintaining excellent properties, being short in production period, high in breeding efficiency, free of influence of regions, seasons and pests and the like, and is generally used for breeding in a culture tank, and the macleaya cordata is placed in the culture tank for breeding, and in the prior art, various culture tanks and culture bottles exist;
through retrieval, like the novel plant tissue culture bottle of patent No. CN201510942100.0 in the prior art, the bottle cover and the bottle jar are in a split structure, the size of the bottle jar is the pipe diameter, when operation, experiment recording photographing and hardening seedling transplanting are needed, the bottle cover and the bottle jar are separated, and the novel plant tissue culture bottle has the advantages of convenience for operation under direct vision and no damage to roots, stems and leaves of plants.
However when the macleaya cordata tissue need take out the macleaya cordata from the bottle jar after the bottle holds in the palm ingrowth, need pull the macleaya cordata that promotes could just with the cultivation to take out from the bottle jar after separating the bottle cover, such process of taking out causes the damage to the root and the stem of macleaya cordata easily, and it is slower to take out speed, and the operation is comparatively inconvenient.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a macleaya cordata plant tissue culture tank, which solves the problems described in the background art.
The invention relates to a macleaya cordata plant tissue culture tank, which is realized by the following specific technical means: the utility model provides a macleaya cordata plant tissue culture jar, includes the body, the embedding of body bottom has the base, and the upper surface of base is hugged closely to the bottom of body, the middle part of base is provided with the cultivation subassembly of planting macleaya cordata plant tissue, the inside top of body is fixed with the nutrient solution and adds the subassembly, the top of body is fixed with seal assembly.
As a further scheme of the invention: the cultivation component consists of a cultivation pot, a bottom plate, a separation plate and a connecting column;
specifically, the top of base is fixed with cultivates the basin, the inside below embedding of cultivateing the basin has the bottom plate, the outside of bottom plate is fixed with the division board, the bottom of bottom plate is fixed with the spliced pole.
As a further scheme of the invention: the upper surface opening of cultivateing the basin is provided with one and hides the groove, bottom plate and separator plate just in time imbed in hiding the groove, the spliced pole of bottom plate below stretches out from the middle part below of base.
As a further scheme of the invention: the adding component consists of a collecting disc, a guide frame, a rotating disc, blades and a spray head;
specifically, the collection tray is fixed with above the inside of body, the upper surface middle part of collection tray is fixed with the positioning disk, the upper surface fixed of positioning disk has the leading truck, the middle part embedding of positioning disk has the rolling disc, the upper surface fixed of rolling disc has the blade, the bottom of rolling disc is fixed with the shower nozzle.
As a further scheme of the invention: the guide frame in the guide disc is arranged in an arc shape with a clockwise opening from inside to outside, the blades in the rotating disc are arranged in an arc shape with a clockwise opening from inside to outside, and the upper surface of the guide disc and the upper surface of the rotating disc are both positioned on the same horizontal plane.
As a further scheme of the invention: the inside vertical trompil of rolling disc is provided with inlet channel, inlet channel sets up between adjacent blade, inlet channel's bottom switches on mutually with the inside of shower nozzle, the middle part of rolling disc and the middle part of rolling disc all are in on the same vertical horizontal plane.
As a further scheme of the invention: the sealing assembly consists of a top plate, a liquid discharge cylinder, an outer frame, a middle plate, a sealing plate, an inner ring, a connecting column and a spring;
specifically, the inside top of body is fixed with the roof, the embedding of the inner wall below of roof has a drainage section of thick bamboo, the inside embedding of roof has outer frame, the middle part of outer frame is fixed with the medium plate, the bottom of outer frame is fixed with the closing plate, the embedding of the inside top of roof has the inner ring, the inside embedding of inner ring has the spliced pole, and the outside nestification of novel spliced pole has the spring.
As a further scheme of the invention: the inside opening of roof is provided with an annular, the inside of drain cylinder and annular is led to mutually, the inboard of annular is hugged closely to the inner wall of closing plate.
As a further scheme of the invention: the top of annular is fixed with the sealing ring, the bottom of sealing ring is hugged closely at the top of outer frame, the outer frame is vertical direction oscilaltion in the annular, the interval between the outer wall of outer frame and the annular is 0.5-1cm.
As a further scheme of the invention: the bottom of the sealing plate is fixed with a baffle plate with the same size as the aperture of the liquid drainage cylinder through a connecting rod, and the baffle plate is just embedded in the lower part inside the liquid drainage cylinder.
The invention provides a macleaya cordata plant tissue culture tank, which has the following beneficial effects:
1. macleaya cordata plant tissue is after the cultivation is accomplished, can carry out the components of a whole that can function independently to body and base, the spliced pole is pressed to follow-up accessible, make spliced pole promote to set up and go up at the bottom plate and the separator plate of cultivateing the basin bottom, let bottom plate and separator plate top move macleaya cordata plant tissue and rise from cultivateing the basin, the realization is fast to separating between cultivateing basin and the macleaya cordata plant tissue, be difficult to in the separation process injure the rhizome of macleaya cordata plant tissue, it is better to the guard effect of macleaya cordata plant tissue, it is more simple and convenient to operate.
2. When nutrient solution added on the medium plate, because gravity nutrient solution promotes the medium plate and moves down, the medium plate promotes the baffle through outer frame and moves down and stretch out from flowing back a liquid section of thick bamboo below, later nutrient solution gets into behind the inside annular of roof along the medium plate, discharge along a flowing back section of thick bamboo, and when no nutrient solution, the spring passes through the spliced pole pulling medium plate and shifts up the recovery normal position, realize that outer frame hugs closely the sealing ring of annular top, in the baffle embedding a liquid section of thick bamboo, and then realize the double seal to the body top, avoid inside the outside air admission device, sealing performance is better.
3. Nutrient solution can enter into the leading truck of positioning disk and discharge when the landing along the catch tray, later gets into the blade of the upper surface striking positioning disk upper surface of rolling disk for the rolling disk is rotatory, and later nutrient solution can get into the cavity passageway discharge from the shower nozzle of rolling disk, realizes that the limit is rotatory to spray the supplementary nutrient solution to the macleaya cordata plant tissue in the limit, reduces and sprays the dead angle, and the nutrient solution interpolation is more even.
Drawings
FIG. 1 is a schematic front sectional view of the overall structure of the present invention;
FIG. 2 is a top view of a partial structure of the bottom plate of the present invention;
FIG. 3 is a schematic top view of a portion of the structure of the steering wheel and the turn disc of the present invention;
FIG. 4 is a schematic top view of a portion of the structure of the guide plate of the present invention;
FIG. 5 is a schematic top view of a partial structure of a rotary plate according to the present invention;
FIG. 6 is a schematic top view showing a partial structure of a culture pot according to the present invention;
FIG. 7 is an enlarged view of FIG. 1A of the present invention.
Illustration of the drawings:
the bottle comprises a bottle body 1, a base 2, a culture pot 201, a bottom plate 202, a separating plate 203, a connecting column 204, a collecting disc 3, a guide disc 301, a guide frame 302, a rotating disc 303, blades 304, a spray head 305, a top plate 4, a liquid discharge cylinder 401, an outer frame 402, a middle plate 403, a sealing plate 404, an inner ring 405, a connecting column 406 and a spring 407.
Detailed Description
Embodiment 1, please refer to fig. 1-7, a macleaya cordata plant tissue culture tank, which comprises a bottle body 1, wherein a base 2 is embedded into the bottom of the bottle body 1, the bottom of the bottle body 1 is tightly attached to the upper surface of the base 2, a cultivation component for planting macleaya cordata plant tissue is arranged in the middle of the base 2, a nutrient solution adding component is fixed above the interior of the bottle body 1, and a sealing component is fixed on the top of the bottle body 1.
The cultivation component consists of a cultivation pot 201, a bottom plate 202, a separation plate 203 and a connecting column 204;
a culture pot 201 is fixed on the top of the base 2, a bottom plate 202 is embedded under the inner part of the culture pot 201, a separation plate 203 is fixed on the outer side of the bottom plate 202, and a connecting column 204 is fixed on the bottom of the bottom plate 202;
the opening on the upper surface of the culture pot 201 is provided with a hidden groove, the bottom plate 202 and the separating plate 203 are just embedded in the hidden groove, and the connecting column 204 below the bottom plate 202 extends out from the lower part of the middle part of the base 2;
the bottle body 1 is connected with the base 2 through bolts, so that the bottle body 1 is more stably connected with the base 2;
macleaya cordata plant tissue is cultivateing to accomplish and carries out the components of a whole that can function independently to body 1 and base 2, spliced pole 204 is pressed to the accessible, make spliced pole 204 promote to set up and move up at bottom plate 202 and the division board 203 of cultivateing the basin 201 bottom, let bottom plate 202 and division board 203 top move macleaya cordata plant tissue and rise from cultivateing the basin, the realization is quick to being separated between cultivateing basin 201 and the macleaya cordata plant tissue, be difficult to among the separation process and injure the rhizome of macleaya cordata plant tissue, it is better to the protection effect of macleaya cordata plant tissue.
Embodiment 2, referring to fig. 1 to 5, embodiment 2 is different from embodiment 1 in that the adding assembly is composed of a collecting tray 3, a guide tray 301, a guide frame 302, a rotating tray 303, blades 304 and a spray head 305;
a collecting disc 3 is fixed above the inner part of the bottle body 1, a guide disc 301 is fixed in the middle of the upper surface of the collecting disc 3, a guide frame 302 is fixed on the upper surface of the guide disc 301, a rotating disc 303 is embedded in the middle of the guide disc 301, blades 304 are fixed on the upper surface of the rotating disc 303, and a spray head 305 is fixed at the bottom of the rotating disc 303;
the guide frame 302 in the guide disc 301 is arranged in an arc shape with a clockwise opening from inside to outside, the blades 304 in the rotating disc 303 are arranged in an arc shape with a clockwise opening from inside to outside, and the upper surface of the guide disc 301 and the upper surface of the rotating disc 303 are both positioned on the same horizontal plane;
after being discharged from the liquid discharge cylinder 401, the nutrient solution slides down along the collection disc 3 and enters the guide disc 301, due to the difference between the upper part and the lower part, the nutrient solution generates gravity and is discharged along the guide frame 302, and the nutrient solution enters the upper surface of the rotating disc 303 and impacts the blades 304 on the upper surface of the rotating disc, so that the rotating disc 303 rotates clockwise;
a liquid inlet channel is vertically arranged in an opening in the rotating disc 303 and is arranged between adjacent blades 304, the bottom of the liquid inlet channel is communicated with the inside of the spray head 305, and the middle of the rotating disc 303 are both positioned on the same vertical horizontal plane;
the nutrient solution can get into the hollow channel of rolling disc 303 and discharge from the shower nozzle afterwards, just in time spray on the macleaya cordata plant tissue of cultivateing the basin 201 and planting, realize the limit rotatory while spraying supplementary nutrient solution to macleaya cordata plant tissue, the nutrient solution interpolation is more even.
Embodiment 3, please refer to fig. 1-7, embodiment 3 is different from embodiment 2 in that the sealing assembly is composed of a top plate 4, a drainage cylinder 401, an outer frame 402, a middle plate 403, a sealing plate 404, an inner ring 405, a connecting column 406 and a spring 407;
a top plate 4 is fixed above the inside of the bottle body 1, a liquid discharging cylinder 401 is embedded below the inner wall of the top plate 4, an outer frame 402 is embedded inside the top plate 4, a middle plate 403 is fixed in the middle of the outer frame 402, a sealing plate 404 is fixed at the bottom of the outer frame 402, an inner ring 405 is embedded above the inside of the top plate 4, a connecting column 406 is embedded inside the inner ring 405, and a spring 407 is nested outside the novel connecting column 406;
an annular groove is formed in an opening in the top plate 4, the liquid discharge cylinder 401 is communicated with the inside of the annular groove, and the inner wall of the sealing plate 404 is tightly attached to the inner side of the annular groove;
the top of the ring groove is fixedly provided with a sealing ring, the top of the outer frame 402 is tightly attached to the bottom end of the sealing ring, the outer frame 402 vertically ascends and descends in the ring groove, and the distance between the outer wall of the outer frame 402 and the ring groove is 0.5-1cm;
the existing space is convenient for the nutrient solution to slide off the outer wall of the middle plate 403;
a baffle plate with the same aperture size as the liquid drainage cylinder 401 is fixed below the bottom end of the sealing plate 404 through a connecting rod, and the baffle plate is just embedded below the inside of the liquid drainage cylinder 401;
when nutrient solution is added to the middle plate 403 of the bottle body 1, the nutrient solution pushes the middle plate 403 to move downwards due to gravity, the middle plate 403 pushes the baffle plate to move downwards through the outer frame 402 to extend out from the lower part of the liquid discharge cylinder 401, the connecting column 406 is pulled to extrude the spring 407 in the inner ring 405, and then the nutrient solution enters the annular groove in the top plate 4 along the middle plate 403 and is discharged along the liquid discharge cylinder 401;
when no nutrient solution exists, the spring 407 pulls the middle plate 403 to move upwards through the connecting column 406 to restore the original position, so that the outer frame 402 is tightly attached to the sealing ring and the baffle above the ring groove and is embedded into the liquid drainage cylinder 401, the top of the bottle body 1 is sealed in a double mode, external air is prevented from entering the inside of the device, and the sealing performance is good;
wherein the middle plate 403, the collecting tray 3, the guide disc 301, the guide frame 302, the rotating disc 303 and the blades 304 are all made of a highly transparent material.
The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make several variations and modifications without departing from the concept of the present invention, and these should be considered as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the utility of the patent.

Claims (10)

1. The utility model provides a macleaya cordata plant tissue culture jar, a serial communication port, including body (1), body (1) bottom embedding has base (2), and the upper surface of base (2) is hugged closely to the bottom of body (1), the middle part of base (2) is provided with the cultivation subassembly of planting macleaya cordata plant tissue, the inside top of body (1) is fixed with the nutrient solution and adds the subassembly, the top of body (1) is fixed with seal assembly.
2. The macleaya cordata plant tissue culture tank of claim 1, wherein the cultivation assembly consists of a cultivation pot (201), a bottom plate (202), a separation plate (203) and a connection column (204);
the top of base (2) is fixed with cultivates basin (201), the inside below embedding of cultivateing basin (201) has bottom plate (202), the outside of bottom plate (202) is fixed with division board (203), the bottom of bottom plate (202) is fixed with spliced pole (204).
3. Macleaya cordata plant tissue culture pot according to claim 2, characterized in that the opening of the upper surface of the culture pot (201) is provided with a hidden groove, the bottom plate (202) and the separating plate (203) are just embedded in the hidden groove, and the connecting post (204) below the bottom plate (202) extends from the lower middle part of the base (2).
4. Macleaya cordata plant tissue culture tank according to claim 1, characterized in that the adding assembly consists of a collecting tray (3), a guiding tray (301), a guiding frame (302), a rotating tray (303), blades (304) and a spray head (305);
the inside top of body (1) is fixed with catch tray (3), the upper surface middle part of catch tray (3) is fixed with positioning disk (301), the upper surface fixed of positioning disk (301) has leading truck (302), the middle part embedding of positioning disk (301) has rolling disc (303), the upper surface fixed of rolling disc (303) has blade (304), the bottom of rolling disc (303) is fixed with shower nozzle (305).
5. The macleaya cordata plant tissue culture pot according to claim 4, wherein the guide frame (302) of the guide disc (301) is formed in a circular arc shape with a clockwise opening from inside to outside, the blades (304) of the rotating disc (303) are formed in a circular arc shape with a clockwise opening from inside to outside, and the upper surface of the guide disc (301) and the upper surface of the rotating disc (303) are on the same horizontal plane.
6. The macleaya cordata plant tissue culture tank according to claim 2 or 4, characterized in that the vertical opening in the rotating disc (303) is provided with a liquid inlet channel, the liquid inlet channel is arranged between the adjacent blades (304), the bottom of the liquid inlet channel is communicated with the inside of the spray head (305), and the middle part of the rotating disc (303) are both on the same vertical horizontal plane.
7. The macleaya cordata plant tissue culture tank of claim 1, wherein the sealing assembly is comprised of a top plate (4), a drainage cylinder (401), an outer frame (402), a middle plate (403), a sealing plate (404), an inner ring (405), a connecting post (406), and a spring (407);
the inside top of body (1) is fixed with roof (4), the embedding of the inner wall below of roof (4) has flowing back section of thick bamboo (401), the inside embedding of roof (4) has outer frame (402), the middle part of outer frame (402) is fixed with medium plate (403), the bottom of outer frame (402) is fixed with closing plate (404), the inside top embedding of roof (4) has inner ring (405), the inside embedding of inner ring (405) has spliced pole (406), and the outside nestification of novel spliced pole (406) has spring (407).
8. The macleaya cordata plant tissue culture pot according to claim 7, wherein the inner opening of the top plate (4) is provided with a ring groove, the drain cylinder (401) is communicated with the inside of the ring groove, and the inner wall of the sealing plate (404) is tightly attached to the inside of the ring groove.
9. The macleaya cordata plant tissue culture tank of claim 7 or 8, wherein the top of the ring groove is fixed with a sealing ring, the bottom end of the sealing ring is tightly attached to the top of the outer frame (402), the outer frame (402) vertically ascends and descends in the ring groove, and the distance between the outer wall of the outer frame (402) and the ring groove is 0.5-1cm.
10. The macleaya cordata plant tissue culture tank according to claim 7 or 8, wherein a baffle plate with the same aperture size as the drainage cylinder (401) is fixed below the bottom end of the sealing plate (404) through a connecting rod, and the baffle plate is just embedded and arranged below the inside of the drainage cylinder (401).
CN202211693614.3A 2022-12-28 2022-12-28 Macleaya cordata plant tissue culture tank Active CN115918541B (en)

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CN114391398A (en) * 2022-01-21 2022-04-26 山东农业工程学院 But split type device of growing seedlings based on wisdom agricultural
CN217546827U (en) * 2022-05-06 2022-10-11 宁明县东安飞腾化肥店 High-efficient forestry seedling culture device
CN217364151U (en) * 2022-06-13 2022-09-06 高州市石生源生物科技发展有限公司 Placement device with protection function for plant tissue culture

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