CN115887535A - Compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis and preparation method thereof - Google Patents
Compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis and preparation method thereof Download PDFInfo
- Publication number
- CN115887535A CN115887535A CN202211172022.7A CN202211172022A CN115887535A CN 115887535 A CN115887535 A CN 115887535A CN 202211172022 A CN202211172022 A CN 202211172022A CN 115887535 A CN115887535 A CN 115887535A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- traditional chinese
- parts
- preventing
- medicine residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 57
- 230000004151 fermentation Effects 0.000 title claims abstract description 57
- 239000003814 drug Substances 0.000 title claims abstract description 53
- 150000001875 compounds Chemical class 0.000 title claims abstract description 35
- 241000271566 Aves Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 12
- 239000001963 growth medium Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 6
- 241000758794 Asarum Species 0.000 claims description 6
- 244000223760 Cinnamomum zeylanicum Species 0.000 claims description 6
- 244000303040 Glycyrrhiza glabra Species 0.000 claims description 6
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 6
- 244000170916 Paeonia officinalis Species 0.000 claims description 6
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 6
- 235000006545 Ziziphus mauritiana Nutrition 0.000 claims description 6
- 244000126002 Ziziphus vulgaris Species 0.000 claims description 6
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims description 6
- 235000017803 cinnamon Nutrition 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 241000722953 Akebia Species 0.000 claims description 5
- 244000061520 Angelica archangelica Species 0.000 claims description 5
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 5
- 235000011477 liquorice Nutrition 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000021110 pickles Nutrition 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 abstract description 11
- 244000144977 poultry Species 0.000 abstract description 9
- 244000144972 livestock Species 0.000 abstract description 7
- 230000001717 pathogenic effect Effects 0.000 abstract description 7
- 108090000695 Cytokines Proteins 0.000 abstract description 6
- 102000004127 Cytokines Human genes 0.000 abstract description 6
- 230000004584 weight gain Effects 0.000 abstract description 6
- 235000019786 weight gain Nutrition 0.000 abstract description 6
- 206010059866 Drug resistance Diseases 0.000 abstract description 4
- 229940124350 antibacterial drug Drugs 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 230000036528 appetite Effects 0.000 abstract description 3
- 235000019789 appetite Nutrition 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 239000003640 drug residue Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000002757 inflammatory effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 18
- 238000012360 testing method Methods 0.000 description 16
- 241000287828 Gallus gallus Species 0.000 description 14
- 210000004369 blood Anatomy 0.000 description 14
- 239000008280 blood Substances 0.000 description 14
- 235000013330 chicken meat Nutrition 0.000 description 14
- 208000002193 Pain Diseases 0.000 description 12
- 230000036407 pain Effects 0.000 description 12
- 210000004185 liver Anatomy 0.000 description 11
- 210000000952 spleen Anatomy 0.000 description 9
- 230000000844 anti-bacterial effect Effects 0.000 description 8
- 235000013594 poultry meat Nutrition 0.000 description 8
- 230000008961 swelling Effects 0.000 description 8
- 229940126680 traditional chinese medicines Drugs 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 210000002216 heart Anatomy 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 6
- 230000000144 pharmacologic effect Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 201000000736 Amenorrhea Diseases 0.000 description 5
- 206010001928 Amenorrhoea Diseases 0.000 description 5
- 208000005171 Dysmenorrhea Diseases 0.000 description 5
- 206010013935 Dysmenorrhoea Diseases 0.000 description 5
- 231100000540 amenorrhea Toxicity 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 231100000331 toxic Toxicity 0.000 description 5
- 230000002588 toxic effect Effects 0.000 description 5
- 206010007247 Carbuncle Diseases 0.000 description 4
- 206010062717 Increased upper airway secretion Diseases 0.000 description 4
- 108090001005 Interleukin-6 Proteins 0.000 description 4
- 108090001007 Interleukin-8 Proteins 0.000 description 4
- 208000005392 Spasm Diseases 0.000 description 4
- 206010042674 Swelling Diseases 0.000 description 4
- 238000011888 autopsy Methods 0.000 description 4
- 230000036772 blood pressure Effects 0.000 description 4
- 210000001669 bursa of fabricius Anatomy 0.000 description 4
- 230000007812 deficiency Effects 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 208000026435 phlegm Diseases 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000002633 protecting effect Effects 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 208000006820 Arthralgia Diseases 0.000 description 3
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 230000000259 anti-tumor effect Effects 0.000 description 3
- 206010003549 asthenia Diseases 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 3
- 235000019605 sweet taste sensations Nutrition 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 208000004998 Abdominal Pain Diseases 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 238000003794 Gram staining Methods 0.000 description 2
- 208000034507 Haematemesis Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 206010019909 Hernia Diseases 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 102100037850 Interferon gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 206010033557 Palpitations Diseases 0.000 description 2
- 206010040943 Skin Ulcer Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000000748 cardiovascular system Anatomy 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000000916 dilatatory effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 208000001780 epistaxis Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000008855 peristalsis Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 231100000019 skin ulcer Toxicity 0.000 description 2
- 210000002460 smooth muscle Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000002936 tranquilizing effect Effects 0.000 description 2
- 230000008736 traumatic injury Effects 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 description 1
- LQIAZOCLNBBZQK-UHFFFAOYSA-N 1-(1,2-Diphosphanylethyl)pyrrolidin-2-one Chemical compound PCC(P)N1CCCC1=O LQIAZOCLNBBZQK-UHFFFAOYSA-N 0.000 description 1
- 241000213006 Angelica dahurica Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 206010008796 Chromaturia Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010726 Conjunctival oedema Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 241001274613 Corvus frugilegus Species 0.000 description 1
- 206010013954 Dysphoria Diseases 0.000 description 1
- 208000010228 Erectile Dysfunction Diseases 0.000 description 1
- 241001360526 Escherichia coli ATCC 25922 Species 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 206010060710 Galactostasis Diseases 0.000 description 1
- 235000017443 Hedysarum boreale Nutrition 0.000 description 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 description 1
- 208000004356 Hysteria Diseases 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 206010025421 Macule Diseases 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000256259 Noctuidae Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010068319 Oropharyngeal pain Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000003288 anthiarrhythmic effect Effects 0.000 description 1
- 230000001773 anti-convulsant effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000001387 anti-histamine Effects 0.000 description 1
- 230000000702 anti-platelet effect Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003416 antiarrhythmic agent Substances 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 229940125715 antihistaminic agent Drugs 0.000 description 1
- 239000000739 antihistaminic agent Substances 0.000 description 1
- 208000002399 aphthous stomatitis Diseases 0.000 description 1
- 230000006793 arrhythmia Effects 0.000 description 1
- 206010003119 arrhythmia Diseases 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 208000012839 conversion disease Diseases 0.000 description 1
- 238000010227 cup method (microbiological evaluation) Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000003314 glucocorticoidlike Effects 0.000 description 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 238000002690 local anesthesia Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000002395 mineralocorticoid Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000004220 muscle function Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 239000007923 nasal drop Substances 0.000 description 1
- 229940100662 nasal drops Drugs 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 208000008494 pericarditis Diseases 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 235000019615 sensations Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 208000023409 throat pain Diseases 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis and a preparation method thereof, belonging to the technical field of traditional Chinese medicine residue fermentation liquids. The compound traditional Chinese medicine residue fermentation liquid is prepared by fermenting lactobacillus rhamnosus PC30-2-1, and can reduce the level of inflammatory cytokines of pathogenic escherichia coli of artificially infected birds, improve bursal index, improve appetite and disease resistance of livestock and poultry, promote weight gain, improve production benefit, reduce or replace the use of conventional antibacterial drugs, reduce drug residues, reduce the selection pressure of the antibacterial drugs, and inhibit or slow down the generation of drug resistance of clinical pathogenic bacteria.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine residue fermentation liquor, and particularly relates to compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis and a preparation method thereof.
Background
Colibacillosis is a common infectious disease of various livestock and poultry, and pathogenic Escherichia coli (e.coli) is an important zoonotic pathogenic bacterium. Not only causes huge economic loss to the poultry breeding industry and hinders the sustainable healthy development of the poultry breeding industry, but also easily causes the safety problem of animal food and brings potential threat to the health and living environment of human beings. At present, the disease is mainly prevented and treated clinically by virtue of antibacterial drugs, but due to unreasonable use of the antibacterial drugs, multiple drug-resistant, cross-resistant and even super-drug-resistant strains continuously appear, and great difficulty is brought to prevention and treatment of the disease. For this reason, reducing or eliminating the resistance of pathogenic E.coli is critical for effective control of the disease. The traditional Chinese medicine has various biological activities of antibiosis, antivirus, immunoregulation and the like, is difficult to generate drug resistance due to various components, and has important significance for effectively preventing and treating bacterial diseases of livestock and poultry caused by pathogenic escherichia coli and ensuring human health and ecological environment. With the release of related regulations such as national veterinary antibacterial use reduction action schemes (2021-2025) in rural agricultural countries in China, the use of veterinary traditional Chinese medicines to reduce or replace conventional veterinary antibacterial medicines has become a new direction for industrial research hotspot and green and healthy development of animal husbandry. The lactobacillus rhamnosus is a safe and widely-applied probiotic, can tolerate the digestive tract environment of animals, can be planted in the intestinal tracts of people and animals, and has the effects of regulating intestinal flora, preventing and treating diarrhea, removing toxins, preventing decayed teeth, improving the immunity of organisms and the like. It has the characteristics of acid resistance, oxygen resistance and strong planting force, and has higher acceptance at present.
In conclusion, on the basis of processing the traditional Chinese medicines, the lactobacillus fermentation technology is utilized, so that the content of active ingredients of the traditional Chinese medicines can be increased, active ingredients which are not existed in the traditional Chinese medicines can be changed or generated, macromolecular substances of the traditional Chinese medicines can be converted into micromolecules which can be directly absorbed and utilized by organisms, and the disease resistance and the economic benefit of the animal organisms can be further improved.
Disclosure of Invention
The invention aims to provide a compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis and a preparation method thereof.
The invention firstly provides a preparation method of compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis, which comprises the following steps:
the method comprises the following steps: lactobacillus rhamnosus PC30-2-1 is separated and obtained from the commercially available pickle, and strain identification is carried out through 16S rDNA to obtain Lactobacillus rhamnosus PC30-2-1 (Lactobacillus rhamnous PC 30-2-1) with the preservation number of CCTCC NO: M2022083, wherein the preservation unit is as follows: china center for type culture Collection, the collection addresses are: wuhan university in China, the preservation date is: inoculating Lactobacillus rhamnosus PC30-2-1 into culture medium at 37 deg.C and 150r/min for 12-18 hr in 2022 year, 1 month and 17 days to obtain seed culture solution;
step two: slicing herba asari, glycyrrhrizae radix, cortex Cinnamomi, radix Angelicae sinensis, radix Paeoniae Rubra, fructus Jujubae, and caulis Akebiae, decocting in water, and mixing filtrates;
step three: drying the dregs obtained in the step two, adding distilled water into the obtained dry dregs, sterilizing, and cooling to room temperature to obtain dregs culture solution;
step four: inoculating the seed culture solution in the second step into the residue culture solution in the third step, performing shaking culture at 37 ℃ for 12-18h at 150r/min, centrifuging, and collecting fermentation supernatant to obtain compound Chinese medicinal residue fermentation liquid.
Preferably, the culture medium of the first step is an MRS liquid culture medium.
Preferably, the Chinese medicinal raw materials in the second step are as follows: 12-15 parts of asarum, 12-15 parts of liquorice, 12-15 parts of cinnamon, 12-15 parts of angelica, 12-15 parts of red peony root, 12-15 parts of Chinese date and 8-10 parts of akebia stem.
Preferably, the Chinese medicinal raw materials in the second step are as follows: 12 parts of asarum, 12 parts of liquorice, 12 parts of cinnamon, 12 parts of angelica, 12 parts of red peony root, 12 parts of Chinese date and 8 parts of akebia stem. Preferably, in the second step, the decocting times are 2 times, the first decocting time is 1h, and the second decocting time is 40-45min.
Preferably, the drying temperature in the third step is room temperature, and the drying time is 48h.
Preferably, the material-liquid ratio of the dregs of a decoction to the distilled water in the third step is 1.
Preferably, the sterilization temperature in the third step is 121 ℃, and the pressure is 103.4kPa.
Preferably, the step four centrifugation collection comprises: centrifuging the residue fermentation liquid at 4 deg.C and 8000r/min for 10min, vacuum filtering the supernatant with 0.45 μm filter membrane, and rotary evaporating the filtrate at 45 deg.C and 75r/min to obtain 1:3.
The invention also provides the compound traditional Chinese medicine residue fermentation liquor for preventing and treating the avian colibacillosis, which is obtained by the preparation method.
The pharmacology and the drug property of each raw material medicine in the medicine dregs are as follows:
asarum: warm in nature, pungent in flavor and slightly toxic. It enters heart, lung and kidney meridians. Expelling wind, dispersing cold, inducing resuscitation, alleviating pain, warming lung, and eliminating phlegm. Herbs of pungent-warm nature for releasing exterior belong to the category of exterior-releasing herbs. 1. Local anesthesia effect; 2. inhibiting arthritis; 3. bacteriostasis; 4. has effects in improving lipid metabolism, increasing blood sugar, and regulating smooth muscle function. The volatile oil can lower blood pressure of anesthetized animals, and the decoction can raise blood pressure; 5. anti-inflammatory and anticonvulsant effects; 6. relieving pain, tranquilizing, inhibiting fever, and relieving fever; 7. anti-histamines and anti-allergic responses; 8. stimulating respiration.
Licorice root: sweet taste and neutral nature, and has effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and relieving cough, relieving spasm and pain, and relieving drug property. It can be used for treating weakness of spleen and stomach, asthenia, palpitation, short breath, cough, excessive phlegm, spasm and pain of abdomen and limbs, carbuncle, swelling, and skin ulcer. The pharmacological effects include: 1. corticoid-like actions (mineralocorticoid action, glucocorticoid-like action); 2. enhancing nonspecific immunity and cellular immunity, but inhibiting humoral immunity; 3. has effects in protecting liver and relieving spasm; 4. detoxification function; 5. anti-inflammatory; 6. relieving cough and eliminating phlegm; 7. resisting pathogenic microorganisms; 8. anti-arrhythmic; 9. anti-tumor; 10. reducing blood lipid, and resisting atherosclerosis.
Cinnamon: the medicine has pungent and sweet taste, large heat in nature, and has the effects of tonifying fire, supporting yang, guiding fire to the source, dispelling cold, relieving pain, promoting blood circulation and dredging channels, and spleen, kidney, heart and liver channels. Can be used for treating sexual impotence, cold womb, lumbago, gonalgia, asthma due to kidney deficiency, conjunctival congestion, pharyngalgia, psychroalgia of heart and abdomen, vomiting and diarrhea due to deficiency cold, hernia due to cold, amenorrhea, and dysmenorrhea. The pharmacological effects include: 1. has cardiovascular effect (tonifying heart, dilating blood vessel, and lowering blood pressure); 2. acting on digestive system (water decoction can inhibit intestinal peristalsis of mice, oleum Cinnamomi can promote intestinal peristalsis, increase digestive juice secretion, and relieve gastrointestinal spasm pain); 3. anti-ulcer; 4. platelet aggregation resistance and anticoagulation resistance; 5. stimulation of the pituitary-adrenal cortex system; 6. regulating immunity.
Chinese angelica: sweet and pungent in flavor and warm in nature, enter liver, heart and spleen channels, and have the effects of enriching and activating blood, regulating menstruation and relieving pain, and relaxing bowel. It can be used for treating blood deficiency, sallow complexion, dizziness, palpitation, menoxenia, amenorrhea, dysmenorrhea, asthenia cold, abdominal pain, constipation due to intestinal dryness, rheumatic arthralgia, traumatic injury, carbuncle, skin ulcer, etc. The pharmacological effects include: 1. has effects in promoting hematopoiesis of bone marrow, inhibiting platelet aggregation, resisting thrombosis, reducing blood lipid, and resisting atherosclerosis; 2. has effects on cardiovascular system (resisting myocardial ischemia, resisting fibrosis after myocardial infarction, resisting arrhythmia, dilating blood vessel, and lowering blood pressure); 3. exciting or inhibiting uterine smooth muscle; 4. enhancing the immune function of the organism; 5. radiation resistance; 6. damage resistance; 7. protecting liver; 8. and (3) resisting tumors.
Red peony root: bitter in taste and slightly cold in nature. It enters liver meridian. Has effects in clearing away heat and cooling blood; promoting blood circulation and removing blood stasis. Main warm poison is taken to cause speckle; hematemesis and epistaxis; the intestinal wind gives the blood; conjunctival congestion with swelling and pain; carbuncle, swelling and sore; amenorrhea and dysmenorrhea; collapse of the leucorrhea and turbid urine; hypochondriac pain due to stasis; hernia accumulation; injury from the fall. Can be used for treating macula due to toxic heat, hematemesis, epistaxis, conjunctival congestion, swelling and pain, liver depression, hypochondriac pain, amenorrhea, dysmenorrhea, abdominal pain, traumatic injury, carbuncle, swelling, and pyocutaneous disease. It is usually indicated for fever bleeding, blood-shot eyes with swelling and pain, abscess, swelling and sore due to warm disease with invasion of heat into blood system. The pharmacological effects include: 1. an antithrombotic effect; 2. anti-platelet aggregation; 3. reducing blood lipid and resisting arteriosclerosis; 4. effects on the cardiovascular system; 5. anti-tumor effect; 6. has liver protecting effect.
Chinese date: sweet taste and warm nature. It enters spleen and stomach meridians. Tonify middle-jiao and Qi, nourish blood and soothe the nerves. Can be used for treating spleen deficiency, anorexia, asthenia, loose stool, and hysteria of women. The pharmacological effects include: 1. central inhibition; 2. protecting liver; 3. enhancing muscle strength and resisting fatigue; 4. anti-allergy reaction; 5. anti-tumor; 6. tranquilizing and allaying excitement; 7. increasing the cAMP of leucocytes, enhancing the immunity and the like.
B, wood through: bitter taste and cold nature. It enters heart, small intestine and bladder meridians. Clear heat and induce diuresis, activate blood and dredge vessels. It is mainly used for treating dark and red urine, stranguria with turbid urine, edema, dysphoria with smothery sensation in chest, throat pain, aphtha, wind-damp arthralgia, galactostasis, amenorrhea and dysmenorrhea. The pharmacological effects include: 1. promoting urination; 2. antibacterial and antifungal.
The invention has the advantages of
The invention provides a compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis and a preparation method thereof, the compound traditional Chinese medicine residue fermentation liquid is fermented by lactobacillus rhamnosus PC30-2-1, and the compound traditional Chinese medicine residue fermentation liquid can reduce the level of inflammatory cell factors of pathogenic escherichia coli of artificially infected birds, improve bursa of fabricius indexes, improve appetite and disease resistance of livestock and poultry, promote weight gain, improve production benefits, reduce or replace the use of conventional antibacterial agents, reduce drug residues, reduce the selection pressure of the antibacterial agents, and inhibit or slow down the generation of drug resistance of clinical pathogenic bacteria.
The seven traditional Chinese medicines in the prescription of the traditional Chinese medicine residue are common traditional Chinese medicines, have low price, no toxic or side effect on the organism of the sick livestock and poultry, no residue in the livestock and poultry body and no drug resistance. In vitro tests show that the medicine has good inhibition effect on escherichia coli; in vivo experiments show that the compound Chinese medicine residue fermentation liquid has better protection and treatment effects on artificially infected avian pathogenic escherichia coli, and is expected to replace common antibacterial agents for preventing and treating livestock and poultry escherichia coli.
Drawings
FIG. 1 is a bar graph of the growth of mice drenching with the decoction dregs fermented liquid of example 2;
FIG. 2 is a Zhou Zengchong bar graph of a test chicken prepared with the herb residue fermentation broth of example 2;
FIG. 3 is a sectional view of organ pathology;
FIG. 4 is a bar graph of IL-6 levels of cytokines;
FIG. 5 is a bar graph of IL-8 levels of cytokines;
FIG. 6 is a bar graph of the cytokine profile of serum IFN-. Gamma.content.
Detailed Description
Example 1 isolation and identification of Lactobacillus rhamnosus PC30-2-1
(1) Separating and purifying
Placing commercially available pickle juice in a sterile ultra-clean workbench, diluting the sample with normal saline by 10 times gradient, uniformly coating 100 μ L of suitable gradient dilution liquid on MRS culture medium containing 2% calcium carbonate, placing the coated culture medium in an anaerobic jar, and culturing at 37 deg.C for 48h. Selecting different forms of bacterial colonies with obvious calcium-dissolving rings, inoculating in corresponding liquid culture medium for enrichment culture for 24h, streaking again on MRS solid culture medium, separating and purifying for 3 times, selecting single bacterial colony, and performing gram staining. Gram-positive colonies were subjected to enrichment culture and stored at-80 ℃ in MRS liquid medium (containing 20% glycerol). All strains were subcultured 3 times before use. After being purified by MRS separation culture medium, the obtained short rod-shaped thallus is round, milk white, neat in edge, smooth in surface, 1-1.5mm in diameter, paired or chain-forming arranged bacterial colonies, and visible in dispersion and chain-forming arrangement by gram staining microscopy.
(2) 16S rDNA sequencing identification
And (3) selecting a single bacterial colony of the purified bacterial strain to 5mL of liquid culture medium for enrichment culture overnight, and extracting the genomic DNA of the isolated bacterial strain by using a bacterial DNA genome extraction kit of the biological engineering (Shanghai) GmbH.
Using the above genomic DNA as a template, 16S rDNA specific fragments of the isolated strain were PCR-amplified using 16S rDNA universal primers (sense sequence: 27F, 5'-AGAGTTTGATCMTGGCTCAG-3', antisense sequence: 1492R, 5'-TACGGYTACCTTG TTACGACTT-3') synthesized by Biotech, and 50. Mu.L of the PCR reaction system is shown in Table 1-2. The PCR amplification program was set up as follows, pre-denaturation: 94 ℃ for 5min; denaturation: 94 ℃ for 30s; annealing: at 55 ℃ for 30s; extension: 72 ℃ for 90s;30 cycles; end extension: 72 deg.C, 10min.
TABLE 1 PCR reaction System
Tab.1 Reaction system of PCR
Taking 5 mu L of PCR amplification product, detecting by 1% agarose gel electrophoresis, cutting and recovering a sample with a clear band at 1600bp, connecting the gel recovery product with a pMD18-T cloning vector, transforming to DH5 alpha competent cells, and sending the positive cloning plasmid to a biological engineering (Shanghai) limited company for sequencing analysis. And comparing the obtained sequencing result with data in NCBI by using BLAST to determine the species of the NCBI. The 16S rDNA sequence alignment result shows that the consistency of the strain and the L.rhamnosus sequence reaches 99 percent.
Example 2 preparation of Compound Chinese medicinal fermentation broth
The method comprises the following steps: inoculating lactobacillus rhamnosus PC30-2-1 stored in a laboratory into 5mL of sterilized MRS liquid culture medium according to the inoculation amount of 1%, and performing shaking culture for 12h at 37 ℃ at 150r/min to obtain a seed culture solution;
step two: weighing 12g of asarum, 12g of liquorice, 12g of cinnamon, 12g of angelica, 12g of red peony root, 12g of Chinese date and 8g of akebia stem, slicing the traditional Chinese medicines, adding 5L of water, decocting for 60min to 2L, and filtering filtrate by gauze; adding 3L of water again, boiling water to slow fire, decocting for 45min to 1L, and mixing filtrates;
step three: drying the obtained medicine residues, weighing a certain amount of dried medicine residues in a conical flask, adding distilled water according to a material-liquid ratio of 1;
step four: aseptically inoculating the seed culture solution of the first step into the conical flask of the third step according to the volume ratio of 0.5%, carrying out shaking culture at 37 ℃ and 150r/min for 18h, centrifuging the dregs fermentation liquor at 4 ℃ and 8000r/min for 10min, carrying out vacuum filtration on the centrifuged supernatant through a 0.45-micron filter membrane, and carrying out rotary evaporation concentration on the filtrate in a rotary evaporator (45 ℃ and 75 r/min) until the volume ratio is 1:3; obtaining the compound traditional Chinese medicine fermentation liquor.
Example 3 Oxford cup method for testing antibacterial activity of compound traditional Chinese medicine fermentation liquor on escherichia coli standard strain ATCC25922
Inoculating laboratory-preserved ATCC25922 strain to sterilized LB liquid medium at a inoculation amount of 1%, culturing at 37 deg.C and 150r/min under shaking to OD 600 =0.5 (about 10) 8 CFU/mL), and continuously diluting 100 times by using LB liquid culture medium for drug sensitivity test;
uniformly coating 100 mu L of the cultured ATCC25922 strain on an LB solid culture medium plate, uniformly placing a sterilized Oxford cup, respectively adding 200 mu L of the concentrated fermentation liquid prepared in the example 2 into the Oxford cup, and standing and culturing for 12 hours in a constant-temperature incubator at 37 ℃;
observing whether the flat plate has an antibacterial zone or not, and measuring the diameter of the antibacterial zone by using a ruler; the results of an Oxford cup experiment show that the supernatant obtained by concentrating and fermenting the traditional Chinese medicine residues has a certain antibacterial effect on escherichia coli ATCC25922 strain antibacterial rings with the diameter of about 12 mm.
Example 4
Healthy Kunming mice (with the weight of 20 +/-2 g) and 10 females and males per group are selected, the prepared decoction dreg fermentation liquor in the example 2 is drenched for 15 days continuously, 200ul of the decoction dreg fermentation liquor is drenched per day, and the mice are observed to eat, drink water, change of body weight and health conditions;
as shown in figure 1, figure 1 is a bar graph of the growth of mice for preparing the herb residue fermentation liquor in the drenching example 2, and as can be seen from figure 1, the mice fed with the compound Chinese medicine residue fermentation liquor have good drinking water, are brightened by Mao Pingshun, and are lively and have no abnormality. The body weight of mice in the group which is infused with 1% compound traditional Chinese medicine residue fermentation liquid is obviously increased compared with that in the control group 10 days before the test.
Example 5
30 healthy rook chicks of 7 days old (with completed basic immunity) are selected, adapted to the environment for 5 days and set as a test group and a control group, 10 chicks/group with half of male and female. The compound traditional Chinese medicine fermentation liquor of the embodiment 2 of the invention is continuously fed for 21 days and 2 times/day by drinking water according to the addition of 1% and 5%, and the water drinking amount is calculated to ensure that the test chickens are completely drunk within 2 hours each time. On the 22 nd day of the test, the addition of the fermentation liquor is stopped, and 0.5mL of the pathogenic escherichia coli CC1 strain is infected by nasal drops per bird (about 10) 9 CFU/mL). 48h after bacteria attack, the state, clinical symptoms and the like of the test chicken are observed, venous blood is collected under wings, the levels of cytokines such as serum IL-2, IL-6, IL-8, IFN-gamma and the like are detected, 6 test chickens are subjected to autopsy in each group, the organ lesion is observed, and the organ indexes such as spleen, bursa of fabricius and the like are calculated.
Referring to fig. 2, fig. 2 is a Zhou Zengchong bar graph of a test chicken prepared with herb residue fermentation broth of example 2, and it can be seen from fig. 2 that 1% and 5% of the herb residue fermentation broth both promote weight gain of the test chicken to a certain extent. The weight gain effect of the 5% addition group is the best at week 2, and the weight gain effect of the 1% addition group is the best at week 3, but the difference with the control group is not obvious. The results show that the compound traditional Chinese medicine residue fermentation liquor is safe to experimental animals, has no toxic or side effect, and can improve the appetite of the animals to a certain extent, promote weight gain and reduce the feed conversion ratio.
Fig. 3 is a diagram of organ lesion autopsy, wherein a represents a control group (no administration), b represents a group of fermentation liquor addition for autopsy of pericardium and liver, and fig. 3 illustrates that after bacteria attack, the drinking water intake of the test chickens in each group is reduced to different degrees compared with normal times, and the individual chickens in the control group only show depression, increased secretion of eyes and nose, and loose wings, and the overall feeding and mental status of the compound traditional Chinese medicine residue fermentation liquor addition group is slightly better than that of the control group. Pathological changes such as pericardial thickening, turbidity, cellulose pericarditis, liver swelling, bleeding and blood stasis, cellulose exudate on the liver surface and the like can be seen only by the autopsy of individual chickens. The compound Chinese medicinal residue fermentation liquid addition group (fig. 3 b) has fewer chickens with the above symptoms, and the overall symptoms are lighter than those of the control group (fig. 3 a).
FIG. 4 is a histogram of IL-6 levels, FIG. 5 is a histogram of IL-8 levels, FIG. 6 is a histogram of serum IFN- γ levels, and FIGS. 4-6 illustrate that 1% and 5% of the herb residue fermentation broth significantly reduced serum IL-6 levels, as compared to the control; in addition, 1% of the traditional Chinese medicine residue fermentation liquor can also obviously reduce the secretion of cytokines such as serum IL-8, IFN-gamma and the like, and shows that the traditional Chinese medicine residue fermentation liquor has a certain anti-inflammatory effect.
Table 2 shows that the compound Chinese medicine residue fermentation liquid of the present invention has an immune organ index of the test chicken, as shown in table 2, 1% and 5% of the Chinese medicine residue fermentation liquid have a promotion effect on the development of bursa of fabricius of the test chicken, the bursa of fabricius indexes are both higher than those of the control group, but the difference is not significant; while 1% and 5% of the Chinese medicinal residue fermentation liquor reduced the spleen index of the test chicken, and compared with the control group, the spleen index was not significant.
TABLE 2 index of fermentation liquid of compound Chinese medicinal residue on immune organs of experimental chicken%
Claims (10)
1. A preparation method of compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis is characterized by comprising the following steps:
the method comprises the following steps: lactobacillus rhamnosus PC30-2-1 is separated and obtained from the commercially available pickle, the strain identification is carried out through 16S rDNA, and the Lactobacillus rhamnosus PC30-2-1 (Lactobacillus rhamnous PC 30-2-1) with the preservation number of CCTCC NO: M2022083 is obtained, wherein the preservation unit is as follows: china center for type culture Collection, the collection addresses are: the preservation date of Wuhan university in China is as follows: inoculating Lactobacillus rhamnosus PC30-2-1 into culture medium at 37 deg.C and 150r/min for 12-18 hr in 2022 year, 1 month and 17 days to obtain seed culture solution;
step two: slicing herba asari, glycyrrhrizae radix, cortex Cinnamomi, radix Angelicae sinensis, radix Paeoniae Rubra, fructus Jujubae, and caulis Akebiae, decocting in water, and mixing filtrates;
step three: drying the dregs obtained in the step two, adding distilled water into the obtained dry dregs, sterilizing, and cooling to room temperature to obtain dregs culture solution;
step four: inoculating the seed culture solution in the second step into the residue culture solution in the third step, performing shaking culture at 37 ℃ for 12-18h at 150r/min, centrifuging, and collecting fermentation supernatant to obtain compound Chinese medicinal residue fermentation liquid.
2. The method for preparing the compound Chinese medicine residue fermentation liquid for preventing and treating the avian colibacillosis according to claim 1, wherein the culture medium in the first step is an MRS liquid culture medium.
3. The preparation method of the compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis according to claim 1, wherein the mixture ratio of the traditional Chinese medicine raw materials in the second step is as follows: 12-15 parts of asarum, 12-15 parts of liquorice, 12-15 parts of cinnamon, 12-15 parts of angelica, 12-15 parts of red peony root, 12-15 parts of Chinese date and 8-10 parts of akebia stem.
4. The preparation method of the compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis according to claim 3, wherein the traditional Chinese medicine raw materials in the step two are in the following proportion: 12 parts of asarum, 12 parts of liquorice, 12 parts of cinnamon, 12 parts of angelica, 12 parts of red peony root, 12 parts of Chinese date and 8 parts of akebia stem.
5. The preparation method of the compound traditional Chinese medicine residue fermentation liquor for preventing and treating avian colibacillosis according to claim 1, wherein the number of times of decoction in the second step is 2, the first decoction time is 1h, and the second decoction time is 40-45min.
6. The preparation method of the compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis according to claim 1, wherein the drying temperature in the third step is room temperature, and the drying time is 48 hours.
7. The preparation method of the compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis according to claim 1, wherein the material-liquid ratio of the traditional Chinese medicine residue to the distilled water in the third step is 1.
8. The preparation method of the compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis according to claim 1, wherein the sterilization temperature in the third step is 121 ℃, and the pressure is 103.4kPa.
9. The preparation method of the compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis according to claim 1, wherein the centrifugal collection in the fourth step comprises: centrifuging the residue fermentation liquid at 4 deg.C and 8000r/min for 10min, vacuum filtering the supernatant with 0.45 μm filter membrane, and rotary evaporating the filtrate in rotary evaporator (45 deg.C and 75 r/min) to volume ratio of 1:3.
10. The compound traditional Chinese medicine residue fermentation liquid for preventing and treating avian colibacillosis, which is obtained by the preparation method of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211172022.7A CN115887535B (en) | 2022-09-26 | 2022-09-26 | Compound Chinese medicinal residue fermentation liquor for preventing and treating colibacillosis of poultry and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211172022.7A CN115887535B (en) | 2022-09-26 | 2022-09-26 | Compound Chinese medicinal residue fermentation liquor for preventing and treating colibacillosis of poultry and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115887535A true CN115887535A (en) | 2023-04-04 |
| CN115887535B CN115887535B (en) | 2023-09-08 |
Family
ID=86471562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211172022.7A Active CN115887535B (en) | 2022-09-26 | 2022-09-26 | Compound Chinese medicinal residue fermentation liquor for preventing and treating colibacillosis of poultry and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115887535B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118064335A (en) * | 2024-04-22 | 2024-05-24 | 晏龙国科(山东)微生物科技有限公司 | Probiotic bacteria and composition with function of relieving nonalcoholic fatty liver and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805275A (en) * | 2012-08-31 | 2012-12-05 | 山东新希望六和集团有限公司 | Feed and traditional Chinese medicine compound used for treating colibacillosis of chicken and preparation method of feed and traditional Chinese medicine compound |
-
2022
- 2022-09-26 CN CN202211172022.7A patent/CN115887535B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805275A (en) * | 2012-08-31 | 2012-12-05 | 山东新希望六和集团有限公司 | Feed and traditional Chinese medicine compound used for treating colibacillosis of chicken and preparation method of feed and traditional Chinese medicine compound |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118064335A (en) * | 2024-04-22 | 2024-05-24 | 晏龙国科(山东)微生物科技有限公司 | Probiotic bacteria and composition with function of relieving nonalcoholic fatty liver and application thereof |
| CN118064335B (en) * | 2024-04-22 | 2024-08-02 | 晏龙国科(山东)微生物科技有限公司 | Probiotic bacteria and composition with function of relieving nonalcoholic fatty liver and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115887535B (en) | 2023-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103689296B (en) | Feed for ewe at later pregnancy and lactation period and preparation method thereof | |
| CN112568324B (en) | Composite probiotics fermented traditional Chinese medicine feed additive and preparation method and application thereof | |
| CN106924477B (en) | Composite traditional Chinese medicine fermentation preparation produced by mixed bacteria fermentation and preparation method thereof | |
| CN114403320A (en) | Lactobacillus fermentum, fermented beverage with weight-losing function and preparation method of fermented beverage | |
| CN115887535B (en) | Compound Chinese medicinal residue fermentation liquor for preventing and treating colibacillosis of poultry and preparation method thereof | |
| CN106309506B (en) | Probiotics composition with menstruation regulating function and application thereof | |
| CN104489687A (en) | Healthcare food containing lactobacillus paracasei for treating fatty liver | |
| KR20090056383A (en) | Composition for prevention or treatment of liver toxicity comprising lactic acid bacteria fermented ssangwhatang | |
| CN109207389B (en) | Thrombolytic lipid-lowering probiotic compound bacteria traditional Chinese medicine oral liquid and preparation method thereof | |
| CN104605345A (en) | Healthcare food containing lactobacillus paracasei for treatment of hyperlipidemia | |
| CN107412713A (en) | Treat Chinese patent drug, dietetic food and the preparation method of autoimmune and immune related diseases | |
| CN105749099B (en) | A kind of probiotics fermention Chinese medicine composition and its preparation method and application for treating gastric cancer | |
| CN111298020B (en) | Traditional Chinese medicine composition with anti-fatigue effect and preparation method and application thereof | |
| CN1682842A (en) | Chinese medicine composition for diminishing-inflammation and detoxication and its preparing method and use | |
| CN103417653B (en) | Veterinary-use compound pure Chinese herbal medicine for treating coccidium and preparation method of oral liquid of same | |
| CN107586746A (en) | One plant of lactic acid bacteria with preventing and treating mastitis for milk cows effect and its application | |
| CN115895941B (en) | Lactobacillus rhamnosus and application thereof | |
| CN111557447A (en) | Preparation of dendrobium officinale polysaccharide buccal tablet and application of dendrobium officinale polysaccharide buccal tablet in immune enhancement | |
| CN103690771B (en) | Be used for the treatment of medicine of lamb colibacillosis and preparation method thereof | |
| CN115607614B (en) | Traditional Chinese medicine composition for treating colibacillosis of livestock and poultry and preparation method thereof | |
| KR20110085872A (en) | Composition for fermentating rice-bran containing bacillus sp | |
| CN108635560A (en) | A kind of Chinese medicine and preparation method thereof for treating lymthoma | |
| CN110754662B (en) | Yellow blood silkworm cordyceps militaris chicken essence electuary suitable for people prone to fatigue and preparation method thereof | |
| CN118649191A (en) | Traditional Chinese medicine composition for protecting liver and intestines of aquatic animals and preparation method thereof | |
| CN107233477A (en) | A kind of composition, preparation method and application method for being used to treat porcine respiratory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |