CN115825460A - Full-automatic chemiluminescence immunoassay analyzer - Google Patents

Abstract

The invention discloses a full-automatic chemiluminescence immunoassay analyzer, which belongs to the field of immunoassay analyzers and comprises an analyzer main body, wherein the analyzer main body consists of an automatic cup feeding module, a manipulator module, a cup shifting module, an AB liquid module, an incubation disc module, a reagent sample sampling needle module, a cleaning pool module, a reagent disc module, an automatic sample feeding module, a magnetic separation cleaning module, a vibration shaking module, a switch door module, a photometric module, a solid-liquid separation module, an industrial personal computer module, a cup losing module and a liquid path module which are arranged on an experiment frame. The invention can realize the functions of automatically replacing samples and reagents, automatically losing cups, cleaning and the like through the cooperation of all modules, can realize the full-automatic whole process of luminescence immunoassay, can obviously improve the working efficiency of the luminescence immunoassay, can realize a real full-automatic chemiluminescence immunoassay analyzer, can effectively ensure the accuracy of the analysis process and the result, and reduce the human errors.

Description

Full-automatic chemiluminescence immunoassay analyzer

Technical Field

The invention relates to the technical field of immunoassay analyzers, in particular to a full-automatic chemiluminescence immunoassay analyzer.

Background

Chemiluminescence immunoassay (CLIA) is a detection and analysis technique for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, drugs and the like by combining a chemiluminescence assay technique with high sensitivity and high specificity immunoreaction. A new immunoassay technique, chemiluminescence immunoassay, developed after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and time-resolved fluorescence immunoassay, is an immunoassay method in which an antigen or an antibody is directly labeled with a chemiluminescent agent. The chemiluminescent substance commonly used for labeling is acridinium ester compound, namely acridinium ester (ae), which is an effective luminescent label and emits light by the action of a starting luminescent reagent (NaOH, H2O 2), and strong direct luminescence is completed within one second and is rapid scintillation. The acridinium ester is used as a marker for immunoassay, and the chemical reaction is simple and quick without a catalyst; the small molecule antigen is detected by a competition method, the large molecule antigen principle adopts a sandwich method, the nonspecific binding is less, and the background is low; binding to macromolecules does not reduce the amount of light produced, thereby increasing sensitivity.

The existing chemiluminescence immunoassay analyzer comprises a large-flux full-automatic chemiluminescence immunoassay analyzer and a small-flux semi-automatic chemiluminescence immunoassay analyzer, the large-flux full-automatic chemiluminescence immunoassay analyzer can realize the in-and-out of samples, the whole process is unattended, the real automation can be realized, the test flux is large, the volume size is large, the transportation is inconvenient, the large-flux chemiluminescence immunoassay analyzer is usually presented in a cabinet type form, the occupied space is large, the large-flux chemiluminescence immunoassay analyzer cannot be placed on a test bed of a small department, and the price is expensive; the small-flux semi-automatic chemiluminescence immune analyzer is a single-channel and multi-channel semi-automatic analyzer, and has small testing flux and low semi-automatic working efficiency; the sample position and the reagent position are few, and the sample and the reagent need to be frequently replaced, so that the full-automatic chemiluminescence immunoassay analyzer is provided.

Disclosure of Invention

The invention aims to solve the problems that in the prior art, a large-flux full-automatic immunoassay analyzer is generally a cabinet type product, has large volume and size and inconvenient transportation, can not be placed on a laboratory table board of a small department for testing and has high price; the small-flux semi-automatic immunoassay analyzer is generally a single-channel and multi-channel semi-automatic analyzer, and has small testing flux and low semi-automatic working efficiency. The sample position and the reagent position are few, and the sample and the reagent need to be frequently replaced, so the full-automatic chemiluminescence immunoassay analyzer is provided.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a full-automatic chemiluminescence immunoassay appearance, includes the analysis appearance main part, the analysis appearance main part is by installing automatic cup module, manipulator module, group cup module, AB liquid module, hatching dish module, reagent sample sampling needle module, washing pond module, reagent dish module, autoinjection module, magnetic separation washing module, vibration shake even module, switch door module, photometry module, solid-liquid separation module, industrial computer module, lose a cup module and liquid way module and constitute on the experimental frame.

Preferably, the automatic cup feeding module is arranged on one side of the experiment frame, the cup poking module is arranged on one side of the output end of the automatic cup feeding module, and the AB liquid module is arranged on the cup poking module on the side opposite to the automatic cup feeding module.

Preferably, incubate a set of module setting and be dialling one side of a cup module output, just it sets up near wasing pond module department to incubate a set of module opposite side, reagent dish module setting is wasing pond module opposite side, the autoinjection module setting is relative setting at the opposite side of reagent dish module, and washing pond module position.

Preferably, the reagent sample sampling needle module for delivering sample reagents is disposed between the reagent tray module and the incubation tray module.

Preferably, the magnetic separation cleaning module is arranged above the experimental frame, the switch door module is arranged between the magnetic separation cleaning module and the incubation disc module, and the photometric module is arranged on one side of the magnetic separation cleaning module and is linked with the magnetic separation cleaning module.

Preferably, the manipulator module is provided with two positions, one manipulator module is arranged between the magnetic separation cleaning module and the incubation disc module, and the other manipulator module is arranged on the reagent sample sampling needle module.

Preferably, the vibration shaking-up module is arranged on one side of the incubation disc module, and the cup losing module for losing cups is arranged on one side of the incubation disc module.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, through the cooperation of the modules, the functions of automatically replacing a sample and a reagent, automatically losing a cup, cleaning and the like can be realized, the full process of full-automatic luminescence immunoassay can be realized, and the working efficiency of the luminescence immunoassay can be obviously improved.

2. The chemiluminescence immunoassay analyzer has the advantages of reasonable overall structural design, proper distribution, high automation degree, low manufacturing cost and wide application range, is suitable for large, medium and small hospitals, can also be used for community rehabilitation hospitals and the like, is a chemiluminescence immunoassay analyzer capable of realizing real full automation, can effectively ensure the accuracy of analysis processes and results, and reduces human errors.

Drawings

FIG. 1 is a schematic perspective view of a full-automatic chemiluminescence immunoassay analyzer according to the present invention;

FIG. 2 is a schematic top view of a full-automatic chemiluminescence immunoassay analyzer according to the present invention;

fig. 3 is an enlarged schematic view of a portion a in fig. 1.

In the figure: 1. an automatic cup feeding module; 2. a manipulator module; 3. a cup shifting module; 4. an AB liquid module; 5. an incubation tray module; 6. a reagent sample sampling needle module; 7. a cleaning tank module; 8. a reagent disk module; 9. an automatic sample introduction module; 10, a reagent sample sampling needle module and a magnetic separation cleaning module; 11. a shaking module; 12. a switch door module; 13. a light measuring module; 14. a solid-liquid separation module; 15. an industrial personal computer module; 16. a cup losing module; 17. and a liquid path module.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

Examples

Referring to fig. 1-3, the full-automatic chemiluminescence immunoassay analyzer comprises an analyzer main body, wherein the analyzer main body consists of an automatic cup feeding module 1, a manipulator module 2, a cup poking module 3, an AB liquid module 4, an incubation disc module 5, a reagent sample sampling needle module 6, a cleaning pool module 7, a reagent disc module 8, an automatic sample feeding module 9, a magnetic separation cleaning module 10, a vibration shaking module 11, a door opening and closing module 12, a photometric module 13, a solid-liquid separation module 14, an industrial personal computer module 15, a cup losing module 16 and a liquid path module 17 which are arranged on an experimental frame.

The industrial personal computer module 15 and the liquid path module 17 are power sources for controlling and driving the modules on the analyzer body, and automation of the analyzer body can be achieved.

The automatic cup feeding module 1 is arranged on one side of the experimental frame, the cup poking module 3 is arranged on one side of the output end of the automatic cup feeding module 1, and the AB liquid module 4 is arranged on one side, opposite to the automatic cup feeding module 1, of the cup poking module 3;

wherein foretell benefit is, cup module 1 can realize shifting cup module 3 department with reaction cup automatic loading and carrying in the automation, through shifting cup module 3 and carry out adjusting position and angle and reach accurate transport and hatch on the dish module 5, realize the effect of cup in the full-automatic.

Furthermore, the incubation disc module 5 is arranged on one side of the output end of the cup shifting module 3, the other side of the incubation disc module 5 is arranged close to the cleaning pool module 7, the reagent disc module 8 is arranged on the other side of the cleaning pool module 7, and the automatic sample feeding module 9 is arranged on the other side of the reagent disc module 8 and is arranged opposite to the cleaning pool module 7;

it should be noted that the reagent and sample are added to the reagent and sample sampling needle module 6 each time, and then the reagent and sample are cleaned in the cleaning pool module 7, so as to avoid the occurrence of cross contamination of the detection sample.

A reagent sample sampling needle module 6 for conveying a sample reagent is arranged between a reagent disk module 8 and an incubation disk module 5, the reagent sample sampling needle module 6 adds a sample and the reagent into a reaction cup on the incubation disk module 5 for incubation, the manipulator module 2 is arranged at two positions, one manipulator module 2 is arranged between a magnetic separation cleaning module 10 and the incubation disk module 5, the other manipulator module 2 is arranged on the reagent sample sampling needle module 6, and the manipulator module 2 arranged between the magnetic separation cleaning module 10 and the incubation disk module 5 clamps the reaction cup in the incubation disk module 5 and places the reaction cup in a vibration shaking module 11 for shaking;

further, magnetism separation washs module 10 and sets up in the top of laboratory bench, and switch door module 12 sets up and washs module 10 and hatches between the dish module 5 at magnetism separation, and photometry module 13 sets up here and magnetism separation washs module 10 one side to link with it, the even module 11 setting of vibration shaking is in hatching dish module 5 one side, and the cup module 16 that loses for losing the cup sets up in the one side of hatching dish module 5.

After the detection sample is incubated in the incubation disc module 5, the door opening and closing module 12 is opened, the manipulator module 2 transfers the reaction cup after the reaction is completed to the magnetic separation cleaning module 10, and simultaneously the door opening and closing module 12 is opened and closed, and then magnetic separation cleaning, pre-excitation liquid adding, excitation liquid adding and photometry are performed, wherein the photometry is performed through the photometry module 13.

When the immunoassay analyzer is used for detection, the immunoassay analyzer comprises the following steps:

the first step is as follows: preparing, namely placing a sample, a reagent, a pre-excitation liquid, an excitation liquid, a cleaning liquid, reaction cup consumables and the like;

the second step is that: applying for project testing, and conveying a sample tube to a sampling position of a sampling needle by an automatic sampling system;

the third step: the automatic cup feeding module 1 automatically loads the reaction cup to the cup shifting module 3 and shifts the reaction cup to the incubation tray module 5 through the cup shifting module 3;

the fourth step: the reagent sample sampling needle module 6 adds a sample and a reagent into a reaction cup of the incubation disc module 5 for reaction;

the fifth step: in order to fully react, the manipulator module 2 clamps and places the reaction cups in the incubation disc module 5 in the vibration shaking module 11 for shaking;

and a sixth step: the reagent sample sampling needle module 6 which is added with the reagent and the sample needs to be cleaned in the cleaning pool module 7;

the seventh step: after the reaction cup is shaken uniformly, the manipulator module 2 clamps the reaction cup into the incubation disc module 5 for incubation;

eighth step: after the incubation of the incubation disc module 5 is finished, the door opening and closing module 12 is opened, the manipulator module 2 transfers the reaction cup after the reaction is finished to the magnetic separation cleaning module 10, the door opening and closing module 12 is closed, and then magnetic separation cleaning, pre-excitation liquid adding and photometry are carried out;

the ninth step: after the light is measured, the waste liquid is discharged through the solid-liquid separation module 14, the switch door module 12 is opened, the manipulator module 2 transfers the reaction cup after the light is measured to the cup losing module 16 to lose the cup, and the switch door is closed;

the tenth step: the photometry module 13 converts the measured optical signal into an electrical signal, and the electrical signal is transmitted to a screen or a hospital lift system through the industrial personal computer module 15 for reference of a doctor.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (7)

1. The utility model provides a full-automatic chemiluminescence immunoassay appearance, includes the analysis appearance main part, a serial communication port, the analysis appearance main part is by installing automatic cup module (1) of going up on the laboratory shelf, manipulator module (2), dial a cup module (3), AB liquid module (4), hatch a set module (5), reagent sample sampling needle module (6), wash pond module (7), reagent dish module (8), autoinjection module (9), magnetic separation washs module (10), vibration shakes even module (11), switch door module (12), photometry module (13), solid-liquid separation module (14), industrial computer module (15), cup loss module (16) and liquid way module (17) and constitutes.

2. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the automatic cup feeding module (1) is arranged at one side of the experimental frame, the cup poking module (3) is arranged at one side of the output end of the automatic cup feeding module (1), and the AB liquid module (4) is arranged at the side of the cup poking module (3) opposite to the automatic cup feeding module (1).

3. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the incubation tray module (5) is disposed on one side of the output end of the cup-poking module (3), and the other side of the incubation tray module (5) is disposed near the cleaning pool module (7), the reagent tray module (8) is disposed on the other side of the cleaning pool module (7), and the autoinjection module (9) is disposed on the other side of the reagent tray module (8) and is disposed opposite to the cleaning pool module (7).

4. A full-automatic chemiluminescent immunoassay analyzer according to claim 1 wherein the reagent sample sampling needle module (6) for delivering sample reagents is disposed between the reagent tray module (8) and the incubation tray module (5).

5. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein the magnetic separation cleaning module (10) is disposed above the experimental rack, the door opening and closing module (12) is disposed between the magnetic separation cleaning module (10) and the incubation disk module (5), and the photometric module (13) is disposed on one side of the magnetic separation cleaning module (10) and is linked therewith.

6. The automated chemiluminescent immunoassay analyzer of claim 1 wherein the manipulator module (2) is in two positions, one manipulator module (2) being disposed between the magnetic separation washing module (10) and the incubation disc module (5) and the other manipulator module (2) being disposed on the reagent sample sampling needle module (6).

7. The automated chemiluminescent immunoassay analyzer of claim 1 wherein the shaking module (11) is disposed on one side of the incubation tray module (5) and the cup dropping module (16) for dropping cups is disposed on one side of the incubation tray module (5).

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