CN115813824A - Plant extract with wound repair effect and preparation method and application thereof - Google Patents
Plant extract with wound repair effect and preparation method and application thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a plant extract with wound repair effect and a preparation method and application thereof. Extracting the three plants of the Chinese brake herb, the centella asiatica and the bidens pilosa by using the eutectic solvent to obtain plant extracts containing the Chinese brake herb extract, the centella asiatica extract and the bidens pilosa extract. The plant extract can quickly form a gamma membrane on the surface of the wound surface immediately after the sun-drying and medical and cosmetic operations, and inhibit bacteria, thereby effectively maintaining the microenvironment of the wound surface, relieving discomforts such as redness, pruritus and stabbing pain, reducing inflammatory response, and promoting barrier repair so as to avoid more serious pigmentation and scars after inflammation. Meanwhile, the skin care product can replenish moisture in time, remove excessive free radicals generated by ultraviolet rays on the skin, protect the skin from the invasion of oxidative free radicals, strengthen the skin barrier, increase the resistance of the skin to the external environment, and deeply repair and nourish the skin.
Description
Technical Field
The invention relates to the field of skin care products and medical dressings, in particular to a plant extract with wound repair effect, a composition, a preparation method and application thereof.
Background
The skin is the first barrier between the body and the nature, and can prevent the invasion of environmental pathogenic microorganisms and the loss of body fluid.
With the development of modern society, people can choose leisure activities such as going to the seaside or walking in holidays, and before outdoor activities, people begin to perform physical sun protection and chemical sun protection in order to prevent sunburn and sunburn. However, no matter how the sun is protected, ultraviolet rays can generate certain stimulation to the skin, so that the moisture loss of the skin is serious, the skin is lack of water, and the skin barrier is damaged; it also causes a series of inflammatory mediators to be released, and the skin becomes reddish, burned, and fever, and if not repaired in time, the skin may also become photoaged in the past. Meanwhile, as people pursue beauty more and more with the development of society, many people have tried various aesthetic treatments such as facial laser, photon skin rejuvenation, micro-acupuncture, tartaric acid skin activation, etc. in order to have admirable skin, not only the use of daily skin care products has been satisfied. Proper medical and cosmetic treatment can solve a plurality of skin problems and make our skin brand new, but medical and cosmetic operations can also bring different degrees of damage to the skin epidermis, so that skin symptoms such as erythema, edema, pigmentation and the like are easy to appear. Therefore, the method becomes an important research hotspot for the repair after solarization and the repair after medical and cosmetic operations.
The skin is in the golden period of recovery after the sun and after the medical and artistic works within 72 hours. Menthol substances are mostly adopted in the existing after-sun repair products on the market to generate cool feeling of skin, but the burden of sunburn skin is increased. In addition, most of the existing products have the effects of moisturizing, the effect is single, and the inflammatory reaction and cell damage caused by ultraviolet rays are difficult to effectively deal with, so the effect of promoting the skin repair after the sun is not obvious. The existing repair products after medical and artistic work are fewer, trehalose dressing is provided at present, the trehalose dressing can reduce the evaporation of moisture on the surface of the skin and achieve the state of moisturizing and moistening the skin, but the effect is single, and the effects of antibiosis, anti-infection, wound healing and the like are insufficient, so that the problems of long wound repair period, easy generation of secondary infection of the wound and the like are solved.
Therefore, it is important to develop a mild, low-irritation, wide-range-of-action product that promotes skin repair within 72 hours.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a plant extract and a composition with wound repair efficacy, and a preparation method and application thereof, and the main components adopt natural plant sources according to the principles of 'mild use, low irritation' and 'monarch, minister, assistant and guide'. The pteris multifida extract is selected as a main component, and the centella extract and the bidens pilosa extract are used as auxiliary materials, so that a gamma film can be formed on the surface of a wound surface quickly and effectively to inhibit bacteria, the microenvironment of the wound surface is effectively maintained, discomfort symptoms such as redness, pruritus and stabbing pain can be relieved, inflammatory reaction is reduced, barrier repair is promoted, moisture can be supplemented timely, excessive free radicals generated by ultraviolet rays on the skin are removed, and the skin can be repaired inside and outside the skin.
The technical scheme of the invention is as follows:
in a first aspect of the present invention, a method for preparing a plant extract with wound healing efficacy is provided, wherein the method comprises the steps of:
providing phoenix-tail fern, centella asiatica and bidens pilosa;
providing a eutectic solvent;
mixing the phoenix-tail fern, the centella asiatica and the bidens pilosa to obtain a mixture;
crushing and sieving the mixture to obtain powder;
and mixing the powder with the eutectic solvent, performing ultrasonic treatment, centrifuging to remove impurities, and concentrating to obtain the plant extract with the wound repair effect.
Optionally, the weight ratio of the phoenix-tail fern, the centella asiatica and the bidens pilosa is 1-10:0.5-8:0.5-6.
Optionally, the method for preparing the eutectic solvent comprises the following steps: mixing a hydrogen bond acceptor and a hydrogen bond donor according to a preset molar ratio, and heating for a preset time under the conditions of a preset temperature and a preset rotating speed to obtain the eutectic solvent;
the hydrogen bond acceptor is one or two of betaine, L-carnitine and amino acid, and the hydrogen bond donor is one of 1, 3-butanediol, urea, 1, 3-propylene glycol, ethylene glycol and glycerol;
and/or the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1-1:8.
optionally, the powder and the eutectic solvent are mixed in a mass-to-volume ratio of 1g: mixing 25-55 mL.
Optionally, the time of the ultrasonic treatment is 25-120min, and the temperature of the ultrasonic treatment is 60-80 ℃.
In a second aspect of the present invention, a plant extract with wound repair efficacy is provided, wherein the plant extract with wound repair efficacy is prepared by the preparation method of the plant extract with wound repair efficacy.
In a third aspect of the present invention, there is provided a method for preparing a composition with wound healing efficacy, wherein the method comprises the steps of:
the plant extract with the wound repair effect, the pentanediol and the deionized water are mixed, stirred and sieved to obtain the composition with the wound repair effect.
Optionally, the preparation method specifically comprises the following steps in parts by weight:
heating 50-85 parts of deionized water to 50-60 ℃, adding 1-30 parts of plant extract, and uniformly stirring until the mixture is transparent to obtain a solution 1;
and cooling the solution 1 to room temperature, adding 5-20 parts of pentanediol, uniformly stirring, and passing through 200-2000 meshes of gauze to obtain the composition with the wound repair effect.
In a fourth aspect of the present invention, a composition with wound repair efficacy is provided, wherein the composition with wound repair efficacy is prepared by using the preparation method of the composition with wound repair efficacy.
In a fifth aspect of the invention, the invention provides an application of the composition with wound repair efficacy in preparing cosmetics and medical dressings.
Has the advantages that: the invention discloses a plant extract with wound repair effect, a composition, a preparation method and application thereof. The plant extract can quickly form a gamma membrane on the surface of the wound surface immediately after the sun-drying and medical and cosmetic operations, and inhibit bacteria, thereby effectively maintaining the microenvironment of the wound surface, relieving discomforts such as redness, pruritus and stabbing pain, reducing inflammatory response, and promoting barrier repair so as to avoid more serious pigmentation and scars after inflammation. Meanwhile, the skin care product can supplement moisture in time, remove excessive free radicals generated by ultraviolet rays on the skin, protect the skin from being invaded by oxidative free radicals, strengthen the skin barrier, increase the resistance of the skin to the external environment, and deeply repair and nourish the skin.
Drawings
FIG. 1 shows the results of the extraction rate tests obtained by the extraction methods of water, ethanol and different eutectic solvents.
FIG. 2 is a graph based on the results of macrophage cytotoxicity assays.
FIG. 3 is a graph based on the results of keratinocyte cytotoxicity assays.
Figure 4 is based on mast cell cytotoxicity assay results.
Fig. 5 shows the results of the cell scratch test.
FIG. 6 shows the results of the cell proliferation assay.
FIG. 7 shows the results of the gene test of the barrier repair-associated protein.
FIG. 8 shows the results of the hyaluronidase activity inhibition assay.
FIG. 9 shows the results of IL-6 inflammatory factor inhibition assay.
FIG. 10 shows Tivi sensitivity test results.
Fig. 11 is the Tivi700 test image results.
FIG. 12 shows the skin erythema E test results.
Figure 13 is the skin redness a test results.
Detailed Description
The invention provides a plant extract with wound repair efficacy, a composition, a preparation method and an application thereof, and the invention is further described in detail below in order to make the purpose, technical scheme and effect of the invention clearer and more clear. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
At present, products for repairing on the market are mainly of the following two types: firstly, the product mostly uses chemical components; secondly, most products have the effects of moisturizing and keeping moisture, the effect is single, the problem of skin repair is solved from a single angle, the effects of antibiosis, infection resistance, relaxation, wound healing and the like are lacked, and excessive free radicals generated by ultraviolet rays on the skin cannot be eliminated, so that the effect of promoting skin repair is not obvious. The inventor finds that the defects can be overcome by compounding the Chinese brake herb extract, the centella extract and the bidens pilosa. Based on this, the present embodiment aims to provide a plant extract that can be applied to cosmetics and medical dressings, and multiple natural plant extracts (i.e., an pteris multifida extract, a centella asiatica extract, and a bidens pilosa extract) are combined according to a certain ratio, so that the obtained plant extracts can immediately and rapidly form a gamma membrane on the surface of a wound to inhibit bacteria, thereby effectively maintaining the microenvironment of the wound, simultaneously relieving discomfort symptoms such as redness, itching, stabbing pain, and the like, reducing inflammatory response, promoting barrier repair, and the like to solve the skin problem, and thereby more effectively exerting the repair efficacy.
The pteris multifida is the whole herb or root of pteris multifida of pteridaceae, has the efficacies of clearing away heat and toxic material, cooling blood and stopping bleeding, eliminating swelling and detoxifying and the like, is used for treating hepatitis, bacillary dysentery, tonsillitis, carbuncle and sore toxin, externally treating traumatic bleeding, burn and scald (the mechanism of the burn and the scald is anti-infection and pain-relieving, the pain is relieved quickly after the medicine is used, a firm protective membrane can be formed with the wound surface quickly, the early wound surface is prevented from seeping in a large amount, and meanwhile, the invasion of external bacteria can be prevented). Modern pharmacological research shows that the phoenix-tail fern contains flavonoid, terpenoid, sterol, volatile oil, phenylpropanoids and other components, and has the functions of broad-spectrum antibiosis, antivirus and anti-free radical.
Centella asiatica is a dry whole plant of centella asiatica of Umbelliferae, which is called copper coin grass, water chestnut grass, centella asiatica and the like. It is cold in nature and bitter in flavor, and has the actions of clearing heat and promoting diuresis, and removing toxicity and relieving swelling. Centella asiatica contains various triterpenes, and has the effects of whitening, resisting oxidation, resisting inflammation, resisting aging, repairing skin injury, beautifying and protecting skin. Clinical and experimental researches show that the centella asiatica can effectively promote the healing of skin wound and has an inhibiting effect on pathological scars formed by excessive healing.
Bidens pilosa is the whole herb of Bidens pilosa of Compositae. Has the effects of removing toxic substances, relieving swelling, clearing heat, relieving pain, promoting blood circulation, removing blood stasis, regulating qi, and resolving food stagnation. It is often used for gastroenteritis, sunstroke, abdominal pain, bacillary dysentery, common cold, fever, acute laryngitis, stranguria, whitish and turbid urine, hemorrhoid, proctoptosis, stool and urine hemorrhage, diabetes, snake bite, scapulohumeral periarthritis, traumatic injury, arthritis, etc. Modern pharmacological research shows that the bidens pilosa contains flavonoid, polyacetylene, phenolic acid and other components, and has the pharmacological effects of antimalarial, antibacterial, anti-inflammatory, antivirus, antioxidation, blood sugar reduction, liver protection and the like.
The plants are traditionally used as medicines and have the effects of clearing heat and removing toxicity, relieving swelling, healing wound surfaces and the like, and the plant extracts are compounded according to a certain proportion, mutually supplemented and synergized to achieve the repairing effect in various ways.
Specifically, the embodiment of the invention provides a preparation method of a plant extract with wound repair efficacy, which comprises the following steps:
providing phoenix-tail fern, centella asiatica and bidens pilosa;
providing a eutectic solvent;
mixing the phoenix-tail fern, the centella asiatica and the bidens pilosa to obtain a mixture;
crushing and sieving the mixture to obtain powder;
and mixing the powder with the eutectic solvent, performing ultrasonic treatment, centrifuging to remove impurities, and concentrating to obtain the plant extract with the wound repair effect.
In one embodiment, the weight ratio of phoenix-tail fern, centella asiatica and bidens pilosa is 1-10:0.5-8:0.5-6. Efficacy tests of various proportions show that the effect is better within the proportion range, and the effective balance between the efficacy and the cost is achieved.
Preferably, the weight ratio of the phoenix-tail fern to the centella asiatica to the bidens pilosa is 8:5:3. efficacy tests of various proportions show that the effect is best at the proportion, and the maximum balance between the efficacy and the cost is achieved.
Selecting the pteris multifida as a main component, assisting centella and bidens pilosa, and extracting the three compounded plants by using a eutectic solvent to obtain the plant composition. The plant composition can quickly form a gamma membrane on the surface of a wound surface immediately after being dried in the sun and after medical and cosmetic operations, and inhibit bacteria, so that the microenvironment of the wound surface is effectively maintained, discomfort symptoms such as redness, pruritus and stabbing pain can be relieved, inflammatory response is reduced, barrier repair is promoted, and more serious pigmentation and scars after inflammation are avoided. Meanwhile, the skin care product can supplement moisture in time, remove excessive free radicals generated by ultraviolet rays on the skin, protect the skin from being invaded by oxidative free radicals, strengthen the skin barrier, increase the resistance of the skin to the external environment, and deeply repair and nourish the skin.
In one embodiment, the method for preparing the eutectic solvent includes the steps of: and mixing the hydrogen bond acceptor and the hydrogen bond donor according to a preset molar ratio, and heating for a preset time under the conditions of a preset temperature and a preset rotating speed to obtain the eutectic solvent. The eutectic solvent prepared by the method can effectively extract the bracken, the centella asiatica and the bidens pilosa.
In one embodiment, the hydrogen bond acceptor may be one or two of betaine, l-carnitine, amino acid, and the like. In one embodiment, the hydrogen bond donor may be one or two of 1, 3-butanediol, urea, 1, 3-propanediol, ethylene glycol, glycerol, and the like.
The hydrogen bond acceptor and the hydrogen bond donor are combined into a eutectic solvent according to a certain molar ratio, and the solvents are common components in the field of skin care products, so that the plant extract obtained by extraction can be directly used in the skin care products.
In one embodiment, the molar ratio of hydrogen bond acceptor to hydrogen bond donor is 1:1-1:8.
in one embodiment, the predetermined temperature is 60-90 deg.C, the predetermined time is 1-2 hours, and the predetermined rotation speed is 400-800rpm (e.g., 400 rpm). Under this condition, it is possible to ensure that a uniform and transparent eutectic solvent is obtained.
In one embodiment, the powder and the eutectic solvent are mixed in a mass to volume ratio of 1g: mixing 25-55 mL. Within the proportion range, the eutectic solvent can effectively extract the Chinese brake herb, the centella asiatica and the bidens pilosa.
In one embodiment, the time of the sonication is between 25 and 120min and the temperature of the sonication is between 60 and 80 ℃.
In one embodiment, the step of removing impurities by centrifugation and concentrating specifically comprises: centrifuging at 1500-4000rpm (such as 2000 rpm) to obtain supernatant and residue, extracting the residue with the eutectic solvent for 1-3 times (such as 2 times), collecting supernatant, and concentrating. Wherein the centrifugation time is 20-30min.
The embodiment of the invention provides a plant extract with wound repair efficacy, wherein the plant extract with wound repair efficacy is prepared by the preparation method provided by the embodiment of the invention.
The embodiment of the invention provides a preparation method of a composition with wound repair efficacy, which comprises the following steps:
the plant extract with wound repair efficacy, which is disclosed by the embodiment of the invention, is mixed with pentanediol (used as a preservative) and deionized water (used as a diluent), and the mixture is stirred and sieved to obtain the composition with wound repair efficacy.
In one embodiment, the preparation method specifically comprises the following steps by weight:
heating 50-85 parts of deionized water to 50-60 ℃, adding 1-30 parts of plant extract, and uniformly stirring until the mixture is transparent to obtain a solution 1;
and cooling the solution 1 to room temperature, adding 5-20 parts of pentanediol, uniformly stirring, and passing through 200-2000 meshes of gauze to obtain the composition with the wound repair effect.
The embodiment of the invention provides a composition with a wound repair effect, wherein the composition with the wound repair effect is prepared by adopting the preparation method of the composition with the wound repair effect.
The embodiment of the invention provides application of the composition with the wound repair effect in preparation of cosmetics and medical dressings.
This example is based on the principle of "mild use, low irritation", and the extraction of natural plants with eutectic solvents. The pteris multifida extract is selected as a main component, and the centella extract and the bidens pilosa extract are used as auxiliary materials, so that the obtained composition can immediately and quickly form a gamma film on the surface of a wound surface to inhibit bacteria, thereby effectively maintaining the microenvironment of the wound surface, relieving discomfortable symptoms such as redness, pruritus and stabbing pain, reducing inflammatory reaction, promoting barrier repair and the like to solve the problem of skin, and further more effectively exerting the repair effect.
Compared with the prior art, the embodiment has the following technical effects:
(1) The plant extract adopted in the embodiment is obtained by extraction with a eutectic solvent, the obtained components are safe and low in stimulation, the extraction method accords with green environmental protection, the plant extract obtained by extraction does not need to remove the solvent, and can be directly used for preparing the composition with the wound repair effect, and the solvent can also be used in cosmetics and medical dressings;
(2) The composition has the wound repair effect by compounding the pteris multifida extract, the centella asiatica extract and the bidens pilosa extract, and can quickly form a physical barrier on the surface of a wound after solarization and medical and cosmetic operations, effectively maintain the microenvironment of the wound, relieve the symptoms of redness, pruritus and stabbing pain, reduce inflammatory reaction and avoid more serious pigmentation and scars after inflammation. Meanwhile, the skin care product can supplement moisture in time, remove excessive free radicals generated by ultraviolet rays on the skin, protect the skin from being invaded by oxidative free radicals, strengthen the skin barrier, increase the resistance of the skin to the external environment, and deeply repair and nourish the skin.
The composition with the wound repair effect can effectively form a gamma film on the surface of the wound, inhibit bacteria, effectively maintain the microenvironment of the wound, relieve discomforts such as redness, pruritus and stabbing pain, reduce inflammatory reaction, promote barrier repair and the like, and repair the inside and outside of the skin.
The invention is further illustrated by the following specific examples.
Cleaning and crushing 80g of phoenix-tail fern, 50g of centella asiatica and 50g of bidens pilosa, and sieving with a 60-mesh sieve to obtain powder. Mixing 9L-carnitine-glycerol with the powder, performing ultrasonic treatment at 75 deg.C for 100min, centrifuging to remove impurities, and concentrating to obtain plant extract.
The other steps are the same, except that: the L-carnitine-glycerol is replaced by water, ethanol, L-carnitine-1, 3-propylene glycol, betaine-glycerol, and betaine-urea.
The extraction rates obtained by the extraction modes of water, ethanol and different eutectic solvents (the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1. As can be seen from FIG. 1, the extraction rate of flavones from L-carnitine-glycerol was the highest.
Example 1
Cleaning and crushing 80g of phoenix-tail fern, 50g of centella asiatica and 50g of bidens pilosa, and sieving with a 60-mesh sieve to obtain powder. Mixing 9L-carnitine-glycerol with the powder, performing ultrasonic treatment at 75 deg.C for 100min, centrifuging to remove impurities, and concentrating to obtain plant extract.
Example 2
Cleaning 80g of herba Pteridis Multifidae, 50g of herba Centellae and 30g of herba Bidentis Bipinnatae, pulverizing, and sieving with 60 mesh sieve to obtain powder. Mixing 8L-carnitine-glycerol with the powder, performing ultrasonic treatment at 75 deg.C for 100min, centrifuging to remove impurities, and concentrating to obtain plant extract.
Example 3
Cleaning 60g of phoenix-tail fern, 50g of centella asiatica and 30g of bidens pilosa, crushing, and sieving with a 60-mesh sieve to obtain powder. Mixing 7L-carnitine-glycerol with the powder, performing ultrasonic treatment at 75 deg.C for 100min, centrifuging to remove impurities, and concentrating to obtain plant extract.
Test example 1
Safety tests were performed for examples 1-3:
1. based on the macrophage cytotoxicity assay, the results are shown in fig. 2. As can be seen from FIG. 2, the compositions obtained in examples 1 to 3, which are based on macrophages, did not exhibit significant cytotoxicity in the concentration range of 1.25% (v/v).
2. Based on the keratinocyte cytotoxicity assay, the results are shown in fig. 3. As can be seen from FIG. 3, the compositions obtained in examples 1 to 3 did not exhibit significant cytotoxicity in the concentration range of 0.625% (v/v) based on keratinocytes.
3. The results based on the mast cell cytotoxicity assay are shown in figure 4. As can be seen from FIG. 4, the compositions obtained in examples 1 to 3 did not show significant cytotoxicity in the concentration range of 0.0078% ((v/v)) based on mast cells.
4. The results of the chick embryo chorioallantoic membrane blood vessel test are shown in Table 1 below.
TABLE 1 toxicity test results of samples on chick embryo chorioallantoic membrane
As can be seen from the above table, compositions 1-3 are directed to the RC of chick embryo chorioallantoic membrane 50 All were in the range (95% ci from 18.3 to 28.84%) without irritation.
Test example 2
The following efficacy tests were performed for examples 1-3:
1. the cell migration test results are shown in fig. 5.
The relative cell mobility of the PC group (positive control group) and the compositions of examples 1 to 3 was significantly increased compared to the BC group (blank control group), indicating that the compositions obtained in examples 1 to 3 have a certain repair ability, and that the repair ability is: composition 2> composition 1> composition 3.
2. The results of the cell proliferation assay are shown in FIG. 6.
Compared with the BC group, the PC group shows the cell activity increasing trend in 24h, 48h and 72h, and the positive control group has the proliferation effect, which indicates that the test system is effective;
the compositions 1 to 3 showed a tendency of increasing cell viability 24 hours after administration compared to the BC group, indicating that the compositions obtained in examples 1 to 3 have a certain repairing ability, and that: composition 2> composition 1> composition 3.
3. The results of the gene test of the barrier repair-related protein are shown in FIG. 7.
Compared with the BC group, the expression of FLG and LOR genes in the PC group is obviously improved, which shows that the experimental system is effective;
compared with the BC group, the composition has obvious improvement effect on FLG and LOR genes, which indicates that the composition can also play a repairing role in the aspect of skin barrier, and the repairing capability is as follows: composition 1> composition 2> composition 3.
4. Hyaluronidase activity inhibition assay, results are shown in figure 8. As is clear from fig. 8, the compositions obtained in examples 1 to 3 had the effect of inhibiting hyaluronidase activity, and the effect of inhibiting hyaluronidase activity, composition 2> composition 3> composition 1.
5. IL-6 (pg/mL) inflammatory factor inhibition assay results are shown in FIG. 9.
Wherein, BC is a blank control group, NC is a negative control group, and PC (dexamethasone) is a positive control group. When the statistical analysis is carried out by using the 1-test method, compared with the BC group, the significance is represented by #, P-value <0.05 is represented by #, and P-value <0.01 is represented by # #; significance is indicated by x compared to NC group, P-value <0.05 and p.value < 0.01.
And (3) testing results:
compared with a blank control group, the IL-6 secretion level of the negative control group is remarkably increased (P is less than 0.01), which indicates that the experimental stimulation condition is effective;
compared with a negative control group, the IL-6 secretion level of the positive control group is remarkably reduced (P is less than 0.01), which indicates that the positive control group is effective in the experiment;
the compositions obtained in examples 1 to 3 significantly reduced the IL-6 content, had anti-inflammatory effects, and anti-inflammatory effects, as compared to the negative control group: composition 2> composition 3> composition 1.
As can be seen from the results of the tests of 4 and 5, the compositions obtained in examples 1 to 3 have excellent anti-inflammatory activity, and can reduce the skin stress and rapidly relieve the symptoms of swelling caused by vasodilatation due to allergic reaction.
From the test results of 1-5, the overall effect is as follows: composition 2> composition 1> composition 3. Example 2 the amount of Bidens pilosa was reduced based on example 1, so the anti-inflammatory effect, cell proliferation effect, and cell migration were better than those of composition 1, indicating that increasing the amount of Bidens pilosa did not increase the anti-inflammatory and cell proliferation and migration effects, but rather decreased the effects; example 3 the amount of phoenix-tail fern was reduced based on example 2, the expression of FLG and LOR genes, cell proliferation and cell migration effects were all reduced, and the anti-inflammatory effects were almost identical. Therefore, the composition obtained in example 2 is most effective in repairing skin barrier from the viewpoint of the effect of repair ability and economy.
Test example 3
Lactic acid stinging test was performed in alternative example 2, and the results are shown in FIGS. 10-13
1. Tivi sensitivity test:
the blank group has sensitivity (TiVi) fluctuating around the basic value at different test time points, and the difference has no statistical significance (P > 0.05), which indicates that the skin barrier function of the testee keeps stable and consistent in the test process, so the influence of the skin state of the testee on the experimental result is negligible.
After the negative control group is successfully modeled, the skin sensitivity is slowly reduced along with the time, and the difference has statistical significance compared with the negative control group after modeling (P < 0.05); and the difference significance of the skin sensitivity (TiVi) of the negative control group at the time point of 90min after the use is lower than that of the blank group (P < 0.05), which indicates that the skin (the negative control group) has certain repair capacity.
The composition obtained in example 2 showed a significant decrease in the mean sensitivity (TiVi) (p < 0.05) at the time points of 60min and 90min after application, which is better than that of the negative control group, indicating that the composition can reduce skin sensitivity.
The Tivi bar graph (shown in FIG. 10) in combination with the Tivi700 test image (shown in FIG. 11) further illustrates that the composition obtained in example 2 promotes skin soothing and reduces skin sensitivity at the time points of 40min, 60min and 90min after the lactic acid sting test.
2. The skin erythema (E) test, the results are shown in FIG. 12.
The blank group has skin erythema (E) fluctuating around the basic value at different test time points, and the difference has no statistical significance (P > 0.05), which indicates that the skin barrier function of the subjects keeps stable and consistent in the test process, so the influence of the skin state of the subjects on the experimental result is negligible.
After the negative control group is successfully modeled, the skin sensitivity is slowly reduced along with the time, and the difference has statistical significance compared with the negative control group after modeling (P < 0.05); the skin erythema (E) fluctuated around the basic value, and the difference had no statistical significance (P > 0.05), which indicates that the skin reddening condition remained stable and consistent in the test process.
The skin erythema (E) value of the composition obtained in example 2 is obviously reduced (p is less than 0.005) at the time points of 40min, 60min and 90min after the use, and the composition is superior to a negative control group, which shows that the composition has better capability of improving the skin erythema state;
3. the results of the skin redness (a) test are shown in fig. 13.
The blank group had a fluctuation of redness (a @) around the basal value at different test time points, and the difference was not statistically significant (P > 0.05), indicating that the skin barrier function of the subjects remained stable and consistent during the test, and therefore the influence of the skin condition of the subjects on the test results was negligible.
After the negative control group is successfully modeled, the skin sensitivity is slowly reduced along with the time, and the difference has statistical significance compared with the negative control group after modeling (P < 0.05); the redness (a) fluctuated around the basal value, and the difference was not statistically significant (P > 0.05), indicating that the redness of the skin remained stable and consistent during the test.
The redness (a) value of the composition is obviously reduced (p is less than 0.05) at the time points of 40min, 60min and 90min after the composition is used, and the redness (a) value is better than that of a negative control group, so that the composition has better redness removing capability.
The lactic acid sting test is combined with the test results of 1, 2 and 3, so that the composition has better efficacy of removing red and soothing allergy.
Combining the results of the above tests, it is demonstrated that the composition of example 2 can repair skin barrier, relieve skin inflammation, reduce redness caused by inflammatory reaction of skin, and can be used in the field of after-sun repair skin care products.
It will be understood that the invention is not limited to the examples described above, but that modifications and variations will occur to those skilled in the art in light of the above teachings, and that all such modifications and variations are considered to be within the scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of a plant extract with wound repair efficacy is characterized by comprising the following steps:
providing phoenix-tail fern, centella asiatica and bidens pilosa;
providing a eutectic solvent;
mixing the phoenix-tail fern, the centella asiatica and the bidens pilosa to obtain a mixture;
crushing and sieving the mixture to obtain powder;
and mixing the powder with the eutectic solvent, performing ultrasonic treatment, centrifuging to remove impurities, and concentrating to obtain the plant extract with the wound repair effect.
2. A method for preparing a plant extract with a wound repair effect according to claim 1, wherein the weight ratio of the phoenix-tail fern, the centella asiatica and the bidens pilosa is 1-10:0.5-8:0.5-6.
3. The method for preparing a plant extract with wound healing efficacy according to claim 1, wherein the preparation method of the eutectic solvent comprises the steps of: mixing a hydrogen bond acceptor and a hydrogen bond donor according to a preset molar ratio, and heating for a preset time under the conditions of a preset temperature and a preset rotating speed to obtain the eutectic solvent;
the hydrogen bond acceptor is one or two of betaine, L-carnitine and amino acid, and the hydrogen bond donor is one of 1, 3-butanediol, urea, 1, 3-propylene glycol, ethylene glycol and glycerol;
and/or the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1-1:8.
4. a method of preparing a plant extract with wound healing efficacy according to claim 1, wherein the powder and the eutectic solvent are mixed in a mass volume ratio of 1g: mixing 25-55 mL.
5. A method of preparing a plant extract with wound healing efficacy according to claim 1, wherein the time of the ultrasonic treatment is 25-120min, and the temperature of the ultrasonic treatment is 60-80 ℃.
6. A plant extract with wound healing efficacy, which is prepared by the preparation method of the plant extract with wound healing efficacy according to any one of claims 1 to 5.
7. A preparation method of a composition with wound repair efficacy is characterized by comprising the following steps:
mixing the plant extract with wound repair effect as claimed in claim 6, pentanediol and deionized water, stirring, and sieving to obtain the composition with wound repair effect.
8. A method for preparing a composition having wound healing efficacy according to claim 7, wherein the method specifically comprises the steps of:
heating 50-85 parts of deionized water to 50-60 ℃, adding 1-30 parts of plant extract, and uniformly stirring until the mixture is transparent to obtain a solution 1;
and cooling the solution 1 to room temperature, adding 5-20 parts of pentanediol, uniformly stirring, and passing through 200-2000 meshes of gauze to obtain the composition with the wound repair effect.
9. A composition having wound healing efficacy, which is prepared by the method for preparing the composition having wound healing efficacy according to any one of claims 7 to 8.
10. Use of the composition with wound healing efficacy of claim 9 in the preparation of cosmetics and medical dressings.
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| PCT/CN2023/088701 WO2024130908A1 (en) | 2022-12-19 | 2023-04-17 | Plant extract with wound repair effect, preparation method therefor, and use thereof |
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| CN111956539A (en) * | 2020-08-03 | 2020-11-20 | 诺德睿行(广州)电子商务有限公司 | Anti-allergy soothing and skin barrier repairing essence cream |
| KR20220058361A (en) * | 2020-10-30 | 2022-05-09 | (주)리윤바이오 | Cosmetic composition for moisturizing and improvement of skin itching comprising the extract of natural products |
| CN115154376A (en) * | 2022-08-03 | 2022-10-11 | 深圳市萱嘉生物科技有限公司 | Extraction method of phoenix-tail fern extract, phoenix-tail fern extract and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024130908A1 (en) * | 2022-12-19 | 2024-06-27 | 深圳市萱嘉生物科技有限公司 | Plant extract with wound repair effect, preparation method therefor, and use thereof |
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| WO2024130908A1 (en) | 2024-06-27 |
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