CN115812723A - Medicament for preventing and treating tea leaf spot and application method thereof - Google Patents
Medicament for preventing and treating tea leaf spot and application method thereof Download PDFInfo
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- CN115812723A CN115812723A CN202211478188.1A CN202211478188A CN115812723A CN 115812723 A CN115812723 A CN 115812723A CN 202211478188 A CN202211478188 A CN 202211478188A CN 115812723 A CN115812723 A CN 115812723A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a medicament for preventing and treating tea white star disease, which comprises difenoconazole, chitosan oligosaccharide, aqueous extract of hovenia dulcis thunb leaves, ethanol extract of brown algae, glycine and water. The medicament for preventing and treating the tea leaf spot has good prevention and treatment effects on the tea leaf spot, ensures the yield and quality of tea leaves, and increases the income of the tea leaves; and the preparation method is simple, ecological, environment-friendly, stable in control effect and suitable for industrial production and application.
Description
Technical Field
The invention relates to the technical field of pesticide medicaments, in particular to a medicament for preventing and treating tea leaf spot and an application method thereof.
Background
The tea leaf spot is also called as star spot disease and leukoderma, is one of the most serious fungal diseases of global high-altitude tea gardens, and is generated all year round in main tea mountain producing areas in China, the yield of a diseased tea garden is generally reduced by about 10 percent, the yield of a diseased tea garden is reduced by more than 50 percent, and even the bud leaves are completely absent. The tea white star disease is a low-temperature and high-humidity disease, the spring tea period is a full-blown period, infected bud leaves are full of small spots, and the prepared tea has high breakage rate, turbid and dark liquor color, reduced fragrance and bitter taste after being brewed, so that the quality of the finished tea is greatly influenced. And after the tea is infected with the white star disease, the nutrient components of the tea are obviously changed. At present, a medicament for preventing and controlling the tea leaf scald disease is lacked, the phenomenon of blind or improper medication of tea enterprises and tea farmers is common, the medication effect is poor, the pesticide residue exceeds the standard easily, and the healthy development of the tea industry is seriously influenced.
Disclosure of Invention
Therefore, the invention provides a medicament for preventing and treating tea leaf spot, which comprises difenoconazole, chitooligosaccharide, aqueous extract of hovenia dulcis thunb leaves, ethanol extract of brown algae, glycine and water, and the preparation method of the aqueous extract of hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for more than 24h, then cutting up, grinding into powder to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, keeping the temperature of the mixture in a water bath at constant temperature of 60 +/-5 ℃ for more than 5h, then carrying out solid-liquid separation to obtain primary filtrate and filter residue, adding the filter residue into the deionized water again, keeping the temperature of the water bath at constant temperature of 60 +/-5 ℃ for more than 5h for secondary extraction, then carrying out solid-liquid separation to obtain secondary filtrate, combining the primary filtrate and the secondary filtrate, concentrating under reduced pressure, and drying to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for more than 24 hours at 80 ℃, then grinding to obtain brown algae powder, preparing an ethanol aqueous solution, mixing the brown algae powder and the ethanol aqueous solution to form a suspension, carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for heat preservation for 6 hours, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration, and drying the concentrated solution in a drying box at 55 +/-5 ℃ to constant weight to obtain the ethanol extract of the brown algae.
Further, the medicament comprises the following components in parts by weight: 20-25 parts of difenoconazole, 5-10 parts of chitooligosaccharide, 6-8 parts of aqueous extract of hovenia dulcis thunb leaves, 4-5 parts of ethanol extract of brown algae, 1-2 parts of glycine and 100 parts of water.
Further, the solid-liquid mass ratio of hovenia dulcis thunb leaf powder to deionized water is hovenia dulcis thunb leaf powder/deionized water = 1-15, and the solid-liquid mass ratio of filter residue to deionized water again is filter residue/deionized water = 1.
Further, the volume part of the ethanol in the ethanol water solution is 70-75%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1.
The invention also discloses a use method of the medicament for preventing and treating the tea leaf spot, which comprises the following steps: diluting the medicament by 1000 times with clear water to obtain application liquid medicine, dividing the application liquid medicine into two parts of spraying and root application, and applying the medicine twice within the period from the bud of the tea tree to the expansion of the fish leaves, wherein the interval between the two application is 7-9 days.
Further, each application rate is: the dosage of the spraying liquid medicine is 40-70 kg/mu, and the dosage of the root-applying liquid medicine is 120-180 kg/mu.
The invention has the beneficial effects that: the medicament for preventing and treating the tea leaf spot has good prevention and treatment effect on the tea leaf spot, ensures the yield and quality of tea leaves, and increases the income of the tea leaves; and the preparation method is simple, ecological, environment-friendly, stable in control effect and suitable for industrial production and application.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A medicament for preventing and treating tea white star disease comprises difenoconazole, chitooligosaccharide, aqueous extract of hovenia dulcis thunb leaves, ethanol extract of brown algae, glycine and water, and comprises the following components in parts by weight: 20 parts of difenoconazole, 5 parts of chitooligosaccharide, 6 parts of aqueous extract of hovenia dulcis thunb leaves, 4 parts of ethanol extract of brown algae, 1 part of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for 24h, then cutting and grinding to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia dulcis thunb leaf powder added into the deionized water is hovenia dulcis thunb leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Example 2
The medicament for preventing and treating the tea white star disease comprises difenoconazole, chitosan oligosaccharide, an aqueous extract of hovenia dulcis thunb leaves, an ethanol extract of brown algae, glycine and water, and comprises the following components in parts by weight: 22 parts of difenoconazole, 6 parts of chitooligosaccharide, 7 parts of aqueous extract of hovenia dulcis thunb leaves, 4 parts of ethanol extract of brown algae, 1 part of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia acerba leaves in an environment of 80 ℃ for drying for 24h, then cutting up, grinding into powder to obtain hovenia acerba leaf powder, weighing the hovenia acerba leaf powder, adding deionized water into the hovenia acerba leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia acerba leaf powder to the deionized water is hovenia acerba leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Example 3
A medicament for preventing and treating tea white star disease comprises difenoconazole, chitooligosaccharide, aqueous extract of hovenia dulcis thunb leaves, ethanol extract of brown algae, glycine and water, and comprises the following components in parts by weight: 24 parts of difenoconazole, 8 parts of chitosan oligosaccharide, 7 parts of aqueous extract of hovenia dulcis thunb leaves, 5 parts of ethanol extract of brown algae, 2 parts of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for 24h, then cutting and grinding to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia dulcis thunb leaf powder added into the deionized water is hovenia dulcis thunb leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Example 4
A medicament for preventing and treating tea white star disease comprises difenoconazole, chitooligosaccharide, aqueous extract of hovenia dulcis thunb leaves, ethanol extract of brown algae, glycine and water, and comprises the following components in parts by weight: 25 parts of difenoconazole, 10 parts of chitosan oligosaccharide, 8 parts of aqueous extract of hovenia dulcis thunb leaves, 5 parts of ethanol extract of brown algae, 2 parts of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for 24h, then cutting and grinding to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia dulcis thunb leaf powder added into the deionized water is hovenia dulcis thunb leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Comparative example 1
A medicament for comparison comprises difenoconazole, an aqueous extract of hovenia dulcis thunb leaves, an ethanol extract of brown algae, glycine and water, wherein the medicament comprises the following components in parts by weight: 24 parts of difenoconazole, 7 parts of an aqueous extract of hovenia dulcis thunb leaves, 5 parts of an ethanol extract of brown algae, 2 parts of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for 24h, then cutting and grinding to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia dulcis thunb leaf powder added into the deionized water is hovenia dulcis thunb leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the aqueous solution of ethanol is brown algae powder/aqueous solution of ethanol = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Comparative example 2
A medicament for comparison comprises difenoconazole, chitosan oligosaccharide, an ethanol extract of brown algae, glycine and water, wherein the medicament comprises the following components in parts by weight: 24 parts of difenoconazole, 8 parts of chitosan oligosaccharide, 5 parts of an ethanol extract of brown algae, 2 parts of glycine and 100 parts of water. The preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for 24 hours at the temperature of 80 ℃, then grinding the brown algae to obtain brown algae powder, preparing an ethanol aqueous solution, and mixing the brown algae powder and the ethanol aqueous solution to form a suspension, wherein the volume fraction of ethanol in the ethanol aqueous solution is 70%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1; and (3) carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for 6h, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration to 1/5 of the volume before concentration, and then placing in a drying box for drying at 55 +/-5 ℃ to constant weight to obtain the brown algae ethanol extract.
Comparative example 3
The contrast medicament comprises difenoconazole, chitosan oligosaccharide, aqueous extract of hovenia dulcis thunb leaves, glycine and water, and comprises the following components in parts by weight: 24 parts of difenoconazole, 8 parts of chitooligosaccharide, 7 parts of aqueous extract of hovenia dulcis thunb leaves, 2 parts of glycine and 100 parts of water. The preparation method of the aqueous extract of the hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for 24h, then cutting and grinding to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, wherein the solid-liquid mass ratio of the hovenia dulcis thunb leaf powder added into the deionized water is hovenia dulcis thunb leaf powder/deionized water = 1; performing water bath at constant temperature of 60 +/-5 ℃ and keeping the temperature for 5h for secondary extraction, performing solid-liquid separation to obtain a secondary filtrate, combining the primary filtrate and the secondary filtrate, performing reduced pressure concentration to 1/5 of the volume of the non-concentrated filtrate, and drying at 60 ℃ to constant weight to obtain the aqueous extract of the hovenia dulcis thunb leaves.
Comparative example 4
A medicament for comparison comprises difenoconazole, glycine and water, and comprises the following components in parts by weight: 24 parts of difenoconazole, 2 parts of glycine and 100 parts of water.
Example 5
Selecting a plurality of plants with the sizes of 20m in the same planting area 2 Each test cell corresponds to the application of the agents prepared in the above examples and comparative examples, respectively, with an equal amount of clear water as a control zone, and each test was repeated 3 times. The medicament application method comprises the following steps: diluting the medicament by 1000 times with clear water to obtain application liquid medicine, dividing the application liquid medicine into two parts of spraying and root application, and applying the medicament twice within the period from the bud of the tea tree (Fuding white tea) to the unfolding of the fish leaves, wherein the interval between the two times of application is 7 days. The application amount of each time is as follows: the dosage of the spraying liquid medicine is 50 kg/mu, and the dosage of the root-applying liquid medicine is 150 kg/mu. The control situation of the tea leaf spot is investigated on the 5 th day after the second medication. Sampling is carried out at five random points in each test cell, 50 bud tips are taken at each point, the disease degree of one bud with two leaves and three leaves is investigated, the disease degree is graded, and the disease index and the prevention and treatment effect are calculated. The disease grading standard is as follows: grade 0, no scab; grade 1, the lesion area accounts for 1 to 10 percent of the leaf area; grade 2, the disease spots account for 11 to 50 percent of the leaf area; grade 3, the lesion spots account for 51 to 80 percent of the leaf area; grade 4, the lesion spots account for more than 81% of the leaf area. Disease index (%) = [. Sigma (number of disease leaves at each level x number of corresponding disease stages)/(number of total investigated plants x 4)]X 100; control effect (%) = [ (clear water control area disease index-medicament treatment area disease index)) Disease index of clear water control area]X 100. The structure is shown in table 1.
As can be seen from table 1, the medicament for preventing and treating the tea white star disease has a good prevention and treatment effect on the tea white star disease, and the prevention and treatment effect can be as high as 82.17%.
TABLE 1
| Test group | Disease index (%) | Control effect (%) |
| Example 1 | 7.63 | 80.57±1.88 |
| Example 2 | 7.49 | 81.05±1.76 |
| Example 3 | 7.04 | 82.17±1.69 |
| Example 4 | 7.37 | 81.33±1.92 |
| Comparative example 1 | 9.15 | 75.91±2.38 |
| Comparative example 2 | 10.97 | 71.30±3.34 |
| Comparative example 3 | 10.66 | 71.97±3.18 |
| Comparative example 4 | 11.38 | 69.22±2.72 |
The technical solutions provided by the present invention are described in detail above, and for those skilled in the art, the ideas according to the embodiments of the present invention may be changed in the specific implementation manners and the application ranges, and in summary, the content of the present description should not be construed as limiting the present invention.
Claims (6)
1. The medicament for preventing and treating the tea leaf spot is characterized by comprising difenoconazole, chitooligosaccharide, an aqueous extract of hovenia dulcis thunb leaves, an ethanol extract of brown algae, glycine and water, and the preparation method of the aqueous extract of hovenia dulcis thunb leaves comprises the following steps: placing hovenia dulcis thunb leaves in an environment of 80 ℃ for drying for more than 24h, then cutting up, grinding into powder to obtain hovenia dulcis thunb leaf powder, weighing the hovenia dulcis thunb leaf powder, adding deionized water into the hovenia dulcis thunb leaf powder to obtain a mixture, keeping the temperature of the mixture in a water bath at constant temperature of 60 +/-5 ℃ for more than 5h, then carrying out solid-liquid separation to obtain primary filtrate and filter residue, adding the filter residue into the deionized water again, keeping the temperature of the water bath at constant temperature of 60 +/-5 ℃ for more than 5h for secondary extraction, then carrying out solid-liquid separation to obtain secondary filtrate, combining the primary filtrate and the secondary filtrate, concentrating under reduced pressure, and drying to constant weight to obtain an aqueous extract of the hovenia dulcis thunb leaves; the preparation method of the brown algae ethanol extract comprises the following steps: drying brown algae for more than 24 hours at 80 ℃, then grinding to obtain brown algae powder, preparing an ethanol aqueous solution, mixing the brown algae powder and the ethanol aqueous solution to form a suspension, carrying out water bath on the suspension at constant temperature of 60 +/-5 ℃ for heat preservation for 6 hours, carrying out condensation reflux in the heat preservation process, carrying out air cooling to normal temperature after the heat preservation is finished, carrying out solid-liquid separation, carrying out reduced pressure concentration, and drying the concentrated solution in a drying box at 55 +/-5 ℃ to constant weight to obtain the ethanol extract of the brown algae.
2. The medicament for preventing and treating the tea leaf spot as claimed in claim 1, wherein the medicament comprises the following components in parts by weight: 20-25 parts of difenoconazole, 5-10 parts of chitosan oligosaccharide, 6-8 parts of aqueous extract of hovenia dulcis thunb, 4-5 parts of ethanol extract of brown algae, 1-2 parts of glycine and 100 parts of water.
3. The medicament for preventing and treating the tea leaf spot according to claim 1, wherein the solid-liquid mass ratio of hovenia dulcis thunb leaf powder added into deionized water is hovenia dulcis thunb leaf powder/deionized water = 1-10, and the solid-liquid mass ratio of filter residue added into deionized water again is filter residue/deionized water = 1-8.
4. The medicament for preventing and treating the tea leaf spot according to claim 1, wherein the volume portion of the ethanol in the ethanol aqueous solution is 70-75%, and the balance is water; the mixing mass ratio of the brown algae powder to the ethanol aqueous solution is that the ratio of the brown algae powder to the ethanol aqueous solution = 1.
5. The use method of the agent for preventing and treating the tea leaf spot as claimed in any one of claims 1 to 4, which comprises the steps of: diluting the medicament by 1000 times with clear water to obtain application liquid medicine, dividing the application liquid medicine into two parts of spraying and root application, and applying the medicine twice within the period from the bud of the tea tree to the expansion of the fish leaves, wherein the interval between the two application is 7-9 days.
6. A method of using a medicament as claimed in claim 5, wherein each dose is: the dosage of the spraying liquid medicine is 40-70 kg/mu, and the dosage of the root-applying liquid medicine is 120-180 kg/mu.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105764344A (en) * | 2013-05-31 | 2016-07-13 | 诺维信生物农业公司 | Compositions and methods for enhancing germination |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105764344A (en) * | 2013-05-31 | 2016-07-13 | 诺维信生物农业公司 | Compositions and methods for enhancing germination |
Non-Patent Citations (1)
| Title |
|---|
| 杨文波等: "防治茶白星病的杀菌剂筛选及防效评价", 中国植保导刊, no. 5, pages 71 - 73 * |
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