CN115721587A - Collagen compound liquid with skin repairing and anti-aging effects - Google Patents
Collagen compound liquid with skin repairing and anti-aging effects Download PDFInfo
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- CN115721587A CN115721587A CN202211287897.1A CN202211287897A CN115721587A CN 115721587 A CN115721587 A CN 115721587A CN 202211287897 A CN202211287897 A CN 202211287897A CN 115721587 A CN115721587 A CN 115721587A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 27
- 108010035532 Collagen Proteins 0.000 title claims abstract description 27
- 229920001436 collagen Polymers 0.000 title claims abstract description 27
- 150000001875 compounds Chemical class 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 title claims abstract description 15
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 14
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 25
- 235000015097 nutrients Nutrition 0.000 claims abstract description 23
- 239000008367 deionised water Substances 0.000 claims abstract description 19
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 18
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 18
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims abstract description 18
- 239000004005 microsphere Substances 0.000 claims abstract description 17
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 14
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 14
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 14
- 235000006708 antioxidants Nutrition 0.000 claims abstract description 14
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 13
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 13
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 13
- 229940046009 vitamin E Drugs 0.000 claims abstract description 13
- 239000011709 vitamin E Substances 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000003085 diluting agent Substances 0.000 claims abstract description 9
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 9
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 9
- 239000000284 extract Substances 0.000 claims abstract description 9
- 210000002950 fibroblast Anatomy 0.000 claims abstract description 9
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 229960004393 lidocaine hydrochloride Drugs 0.000 claims abstract description 9
- YECIFGHRMFEPJK-UHFFFAOYSA-N lidocaine hydrochloride monohydrate Chemical compound O.[Cl-].CC[NH+](CC)CC(=O)NC1=C(C)C=CC=C1C YECIFGHRMFEPJK-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000011780 sodium chloride Substances 0.000 claims abstract description 9
- 235000003181 Panax pseudoginseng Nutrition 0.000 claims abstract description 7
- 244000131316 Panax pseudoginseng Species 0.000 claims abstract description 7
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 7
- 239000011550 stock solution Substances 0.000 claims description 44
- 238000010438 heat treatment Methods 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 20
- 238000001816 cooling Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 8
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 4
- 230000001804 emulsifying effect Effects 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 2
- AYPZAZPOYROADP-UHFFFAOYSA-N 2-(2-phenylethenyl)phenol Chemical compound OC1=CC=CC=C1C=CC1=CC=CC=C1 AYPZAZPOYROADP-UHFFFAOYSA-N 0.000 claims description 2
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004166 Lanolin Substances 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 235000013793 astaxanthin Nutrition 0.000 claims description 2
- 239000001168 astaxanthin Substances 0.000 claims description 2
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims description 2
- 229940022405 astaxanthin Drugs 0.000 claims description 2
- 235000013871 bee wax Nutrition 0.000 claims description 2
- 239000012166 beeswax Substances 0.000 claims description 2
- 239000005515 coenzyme Substances 0.000 claims description 2
- 229940087603 grape seed extract Drugs 0.000 claims description 2
- 235000002532 grape seed extract Nutrition 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 235000019388 lanolin Nutrition 0.000 claims description 2
- 229940039717 lanolin Drugs 0.000 claims description 2
- 239000000787 lecithin Substances 0.000 claims description 2
- 229940067606 lecithin Drugs 0.000 claims description 2
- 235000010445 lecithin Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 235000020944 retinol Nutrition 0.000 claims description 2
- 229960003471 retinol Drugs 0.000 claims description 2
- 239000011607 retinol Substances 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 239000001717 vitis vinifera seed extract Substances 0.000 claims description 2
- 210000002268 wool Anatomy 0.000 claims description 2
- 230000001815 facial effect Effects 0.000 abstract description 6
- 230000032683 aging Effects 0.000 abstract description 4
- 102000001187 Collagen Type III Human genes 0.000 abstract description 3
- 108010069502 Collagen Type III Proteins 0.000 abstract description 3
- 239000002344 surface layer Substances 0.000 abstract description 3
- 230000017423 tissue regeneration Effects 0.000 abstract 2
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 21
- 102000016942 Elastin Human genes 0.000 description 3
- 108010014258 Elastin Proteins 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 229920002549 elastin Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000003143 Panax notoginseng Nutrition 0.000 description 2
- 241000180649 Panax notoginseng Species 0.000 description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 description 2
- 229940010747 sodium hyaluronate Drugs 0.000 description 2
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000006286 nutrient intake Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- -1 phosphorus ions Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Cosmetics (AREA)
Abstract
In order to solve the problems of dry, dark, aging and the like of the skin, the invention provides the technical scheme that: the collagen compound liquid with the skin repairing and anti-aging effects is characterized by comprising the following raw materials in percentage by weight: 10-20% of human-derived collagen type III, 5-10% of pseudo-ginseng extract, 1-4% of calcium carbonate, 1-4% of disodium hydrogen phosphate, 2-4% of vitamin E, 2-8% of antioxidant, 1-2% of emulsifier, 2-5% of sodium chloride diluent, 3-6% of fibroblast, 5-10% of lidocaine hydrochloride injection, 5-10% of PH regulator and the balance of deionized water. The invention has the advantages that: vitamin E and antioxidant to prevent oxidation of the surface layer of facial skin; hydroxyapatite Dan Zhongkong microspheres are added into the nutrient solution, so that a high-quality environment is provided for the new tissue, and the tissue regeneration and repair are enhanced.
Description
Technical Field
The invention relates to the technical field of skin care, in particular to a collagen compound liquid with skin repairing and anti-aging effects.
Background
The skin is connective tissue, and consists of three parts, namely epidermis, dermis and subcutaneous tissue from outside to inside. The dermis is a reticular structure composed of collagen, elastin, hyaluronic acid, microvasculature and other substances, wherein the collagen and elastin play a supporting role, so that the dermis has good firmness and elasticity. Sodium hyaluronate can be used as moisturizing factor to retain moisture in skin. According to scientific research, the speed of generating sodium hyaluronate and collagen in vivo is reduced from 20 years old, and the elasticity of elastin capable of rapidly rebounding skin is reduced, so that the substances in the skin are lost year by year, the content is reduced to half by 40 years old, skin tissues are shrunk, gaps are formed, and the skin shows aging phenomena such as looseness, wrinkles, dryness, roughness, large pores and the like.
However, the change of the skin is not only simple as the growth of the age, and with the increasing pressure on people in society, people often have difficulty in paying attention to nutrient intake through diet due to lack of time, so that the human body is easy to lose nutrient elements, the loss of the nutrient elements can be directly reflected on the facial skin, so that the phenomena of dry, dull, ageing and the like of the facial skin occur, the existing nutrient solution for skin care pays more attention to temporary protection, and the nutrition supplement on the human body cannot be realized to avoid the skin problem caused by the nutrient loss. Therefore, a collagen compound solution with skin repairing and anti-aging effects is needed to solve the above problems.
Disclosure of Invention
Aims to solve the problems of dry, dark and aging skin and the like. The invention aims to provide a collagen compound liquid with skin repairing and anti-aging effects by utilizing the regeneration and repair characteristics of hydroxyapatite Dan Zhongkong microspheres.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: the collagen compound liquid with the skin repairing and anti-aging effects is characterized by comprising the following raw materials in percentage by weight: 10-20% of human-derived collagen, 5-10% of pseudo-ginseng extract, 1-4% of calcium carbonate, 1-4% of disodium hydrogen phosphate, 2-4% of vitamin E, 2-8% of antioxidant, 1-2% of emulsifier, 2-5% of sodium chloride diluent, 3-6% of fibroblast, 5-10% of lidocaine hydrochloride injection, 5-10% of PH regulator and the balance of deionized water.
Preferably, the antioxidant is at least one of stilbenol, grape seed extract, coenzyme, retinol and astaxanthin; the emulsifier is at least one of lecithin, lanolin alcohol, wool acid and beeswax; the pH regulator is at least one of citric acid, lactic acid, potassium hydroxide, sodium hydroxide and disodium hydrogen phosphate.
Preferably, the vitamin E is a water-soluble vitamin.
A collagen compound liquid with skin repairing and anti-aging effects comprises the following preparation methods:
the method comprises the following steps: preparation of hydroxyapatite Dan Zhongkong microspheres: equal amount of calcium carbonate and disodium hydrogen phosphate are used to prepare the hydroxyapatite Dan Zhongkong microballoon under normal pressure.
Step two: preparing a stock solution A: weighing human collagen, vitamin E, antioxidant and fibroblast according to the weight, putting the human collagen, the vitamin E, the antioxidant and the fibroblast into a reaction vessel for mixing, adding a small amount of deionized water for many times, heating the reaction vessel to 30-45 ℃, completely fusing the raw materials with the deionized water to prepare stock solution A, stopping heating the reaction vessel, and naturally cooling the stock solution A.
Step three: preparing a stock solution B: weighing sodium chloride diluent, lidocaine hydrochloride injection and pseudo-ginseng extract according to the amount, putting the sodium chloride diluent, the lidocaine hydrochloride injection and the pseudo-ginseng extract into a reaction vessel for mixing, adding deionized water in a small amount for multiple times, heating the reaction vessel to 40-60 ℃, completely fusing the raw materials and the deionized water to prepare stock solution B, stopping heating the reaction vessel, and naturally cooling the stock solution B.
Step four: mixing the stock solution A and the stock solution B, pouring the stock solution A and the stock solution B into an emulsifying pot under the aseptic environment of 25-35 ℃, stirring uniformly, and then adding an emulsifier to prepare a sufficient mixture of the stock solution A and the stock solution B.
Step five: adjusting the pH of the mixture: and (3) after the nutrient solution is kept stand for 1-2 hours, adding a pH regulator, regulating the pH value of the mixture to 7-8, and stirring for 15-20 min to obtain the nutrient solution.
Step six: putting the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution into a mixing container, heating the mixing container to 30-45 ℃, stirring the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution for 20-40min to prepare a compound solution, and naturally cooling.
Preferably, the stock solution A in the second step is stored in a sterile environment at 2-10 ℃.
Preferably, the stock solution B in the third step is stored in a sterile environment at the temperature of 2-10 ℃.
The invention has the advantages that:
the vitamin E and the antioxidant can prevent the collagen compound liquid from contacting air to be oxidized after being smeared, and the facial skin is directly contacted with the air through the antioxidant collagen compound liquid to prevent the surface layer of the facial skin from being oxidized;
the hydroxyapatite Dan Zhongkong microspheres are added into the nutrient solution, after the nutrient solution extends into the surface layer of the facial skin, when the nanocluster hydroxyapatite Dan Zhongkong microspheres contact with body fluid, HAP can absorb human bioactive factors, amino acids and proteins simultaneously release calcium ions, phosphorus ions and other human homologous substances to exert physiological functions to release a unique hollow microsphere structure of collagen, and the regenerated collagen is inlaid among tissues in a staggered mode.
Detailed Description
Example 1
The hydroxyapatite Dan Zhongkong microsphere is prepared by 2% calcium carbonate and 2% disodium hydrogen phosphate under normal pressure.
Weighing 12% of human collagen type III, 2% of vitamin E, 4% of antioxidant and 3% of fibroblasts, putting the mixture into a reaction vessel for mixing, adding a small amount of deionized water for many times, heating the reaction vessel to 35 ℃, completely fusing the raw materials and the deionized water to prepare stock solution A, stopping heating the reaction vessel, and naturally cooling the stock solution A.
Weighing 2% sodium chloride diluent, 6% lidocaine hydrochloride injection and 6% notoginseng extract, placing into a reaction vessel, mixing, adding a small amount of deionized water for many times, heating the reaction vessel to 45 deg.C, completely mixing the raw materials with deionized water to obtain stock solution B, stopping heating the reaction vessel, and naturally cooling the stock solution B.
Pouring the stock solution A and the stock solution B into an emulsifying pot under the aseptic environment of 28 ℃, uniformly stirring, and then adding an emulsifier to prepare a sufficient mixture of the stock solution A and the stock solution B.
And (3) standing the nutrient solution for 1 hour, adding a pH regulator, regulating the pH value of the mixture to 7, and stirring for 15min to obtain the nutrient solution.
Putting the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution into a mixing container, heating the mixing container to 35 ℃, stirring the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution for 20min to prepare a compound solution, and naturally cooling.
Example 2
The hydroxyapatite Dan Zhongkong microsphere is prepared by 3 percent of calcium carbonate and 3 percent of disodium hydrogen phosphate under normal pressure.
Weighing 18% of human-derived type III collagen, 4% of vitamin E, 4% of antioxidant and 5% of fibroblasts, putting the mixture into a reaction vessel for mixing, adding a small amount of deionized water for many times, heating the reaction vessel to 40 ℃, completely fusing the raw materials and the deionized water to prepare stock solution A, stopping heating the reaction vessel, and naturally cooling the stock solution A.
Weighing 4% sodium chloride diluent, 8% lidocaine hydrochloride injection and 8% radix Notoginseng extract, mixing in a reaction vessel, adding deionized water in a small amount, heating the reaction vessel to 55 deg.C, mixing the raw materials with deionized water to obtain stock solution B, stopping heating the reaction vessel, and naturally cooling the stock solution B.
Pouring the stock solution A and the stock solution B into an emulsifying pot under the aseptic environment of 32 ℃, uniformly stirring, and then adding an emulsifier to prepare a sufficient mixture of the stock solution A and the stock solution B.
Standing the nutrient solution for 1 hr, adding pH regulator, regulating pH of the mixture to 8, and stirring for 20min to obtain the nutrient solution.
Putting the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution into a mixing container, heating the mixing container to 40 ℃, stirring the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution for 40min to prepare a compound solution, and naturally cooling.
The invention and its embodiments have been described above without limitation. In summary, those skilled in the art should be able to conceive of the present invention without creative design of the similar structural modes and embodiments without departing from the spirit of the present invention, and all such modifications should fall within the protection scope of the present invention.
Claims (6)
1. The collagen compound liquid with the skin repairing and anti-aging effects is characterized by comprising the following raw materials in percentage by weight: 10-20% of human-derived collagen, 5-10% of pseudo-ginseng extract, 1-4% of calcium carbonate, 1-4% of disodium hydrogen phosphate, 2-4% of vitamin E, 2-8% of antioxidant, 1-2% of emulsifier, 2-5% of sodium chloride diluent, 3-6% of fibroblast, 5-10% of lidocaine hydrochloride injection, 5-10% of PH regulator and the balance of deionized water.
2. The collagen compound liquid with skin repairing and anti-aging effects as claimed in claim 1, wherein:
the antioxidant is at least one of stilbenol, grape seed extract, coenzyme, retinol and astaxanthin;
the emulsifier is at least one of lecithin, lanolin alcohol, wool acid and beeswax;
the pH regulator is at least one of citric acid, lactic acid, potassium hydroxide, sodium hydroxide and disodium hydrogen phosphate.
3. The collagen compound liquid with skin repairing and anti-aging effects as claimed in claim 1, wherein: the preparation method comprises the following steps:
the method comprises the following steps: preparation of hydroxyapatite Dan Zhongkong microspheres: equal amount of calcium carbonate and disodium hydrogen phosphate are used to prepare the hydroxyapatite Dan Zhongkong microballoon under normal pressure.
Step two: preparing a stock solution A: weighing human collagen, vitamin E, antioxidant and fibroblast according to the weight, putting the human collagen, the vitamin E, the antioxidant and the fibroblast into a reaction vessel for mixing, adding a small amount of deionized water for many times, heating the reaction vessel to 30-45 ℃, completely fusing the raw materials with the deionized water to prepare stock solution A, stopping heating the reaction vessel, and naturally cooling the stock solution A.
Step three: preparing a stock solution B: weighing sodium chloride diluent, lidocaine hydrochloride injection and pseudo-ginseng extract according to the amount, putting the sodium chloride diluent, the lidocaine hydrochloride injection and the pseudo-ginseng extract into a reaction vessel for mixing, adding deionized water in a small amount for multiple times, heating the reaction vessel to 40-60 ℃, completely fusing the raw materials and the deionized water to prepare stock solution B, stopping heating the reaction vessel, and naturally cooling the stock solution B.
Step four: mixing the stock solution A and the stock solution B, pouring the stock solution A and the stock solution B into an emulsifying pot under the aseptic environment of 25-35 ℃, stirring uniformly, and then adding an emulsifier to prepare a sufficient mixture of the stock solution A and the stock solution B.
Step five: adjusting the pH of the mixture: and (3) after the nutrient solution is kept stand for 1-2 hours, adding a pH regulator, regulating the pH value of the mixture to 7-8, and stirring for 15-20 min to obtain the nutrient solution.
Step six: putting the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution into a mixing container, heating the mixing container to 30-45 ℃, stirring the hydroxyapatite Dan Zhongkong microspheres and the nutrient solution for 20-40min to prepare a compound solution, and naturally cooling.
4. The collagen compound liquid with skin repairing and anti-aging effects as claimed in claim 1, wherein: the vitamin E is water-soluble vitamin.
5. The collagen compound liquid with skin repairing and anti-aging effects as claimed in claim 3, wherein: and the stock solution A in the second step is stored in a sterile environment at the temperature of 2-10 ℃.
6. The collagen compound liquid with skin repairing and anti-aging effects as claimed in claim 3, wherein: and the stock solution B in the third step is stored in a sterile environment at the temperature of 2-10 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211287897.1A CN115721587A (en) | 2022-10-20 | 2022-10-20 | Collagen compound liquid with skin repairing and anti-aging effects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211287897.1A CN115721587A (en) | 2022-10-20 | 2022-10-20 | Collagen compound liquid with skin repairing and anti-aging effects |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104961115A (en) * | 2015-06-24 | 2015-10-07 | 浙江理工大学 | Hollow hydroxyapatite microsphere and preparation method thereof |
| CN106420395A (en) * | 2016-11-15 | 2017-02-22 | 宁波希诺亚海洋生物科技有限公司 | Anti-allergy regenerative mask fluid |
| CN112168853A (en) * | 2019-07-05 | 2021-01-05 | 上海春芝堂生物制品有限公司 | Coenzyme Q10 pseudo-ginseng composite tablet with antioxidant function and preparation method thereof |
| CN114632056A (en) * | 2022-03-31 | 2022-06-17 | 苏州芳华美容医院有限公司 | Compound nutrient solution for facial anti-aging |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104961115A (en) * | 2015-06-24 | 2015-10-07 | 浙江理工大学 | Hollow hydroxyapatite microsphere and preparation method thereof |
| CN106420395A (en) * | 2016-11-15 | 2017-02-22 | 宁波希诺亚海洋生物科技有限公司 | Anti-allergy regenerative mask fluid |
| CN112168853A (en) * | 2019-07-05 | 2021-01-05 | 上海春芝堂生物制品有限公司 | Coenzyme Q10 pseudo-ginseng composite tablet with antioxidant function and preparation method thereof |
| CN114632056A (en) * | 2022-03-31 | 2022-06-17 | 苏州芳华美容医院有限公司 | Compound nutrient solution for facial anti-aging |
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