CN115671206B - Traditional Chinese medicine peptide oral liquid for relieving fatigue and preparation method thereof - Google Patents
Traditional Chinese medicine peptide oral liquid for relieving fatigue and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of traditional Chinese medicine preparation, and in particular discloses a traditional Chinese medicine peptide oral liquid for relieving fatigue and a preparation method thereof. The traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following raw material components in parts by weight: 15-25 parts of American ginseng, 10-20 parts of rhodiola rosea, 10-15 parts of centella asiatica, 5-10 parts of black mulberry, 4-8 parts of morinda officinalis, 2-6 parts of passion flower, 2-4 parts of peach gum, 5-10 parts of fragrant solomonseal rhizome, 8-12 parts of bighead atractylodes rhizome and 15-30 parts of red date. The lactobacillus rhamnosus and the bifidobacterium longum are adopted to ferment the traditional Chinese medicine components twice, so that the nutrition components and the active components in the traditional Chinese medicine components are fully released, a large amount of active peptide components are generated in the fermentation process, the absorption and the utilization of the active components in the traditional Chinese medicine are facilitated, the intestinal burden of a human body is relieved, the fatigue relieving effect of the formula is improved, in addition, the prepared traditional Chinese medicine peptide oral liquid has good taste, the crowd compliance is improved, and the market prospect is wide.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a traditional Chinese medicine peptide oral liquid for relieving fatigue and a preparation method thereof.
Background
Fatigue, a physiological phenomenon, means that the physiological process of the body cannot last for its function at a certain level and/or cannot maintain a predetermined exercise intensity. With the acceleration of modern life rhythm and the increase of working pressure, the bodies of many people are in sub-health state, namely, the bodies are free from organic lesions, but functional disorders, such as fatigue, poor spirit, low immunity, easy to cause common diseases, natural aging acceleration and other symptoms, are in the early stages of cardiovascular and cerebrovascular diseases or other chronic diseases, are generated. If the sub-health state is not improved in time, the problems of abnormal regulation of the nervous system and the endocrine system of the organism and the like can be caused, and finally, organic lesions are generated.
Along with the continuous improvement of living standard, people's health consciousness and health preserving concept are stronger and stronger, and the disease prevention is changed from the past disease treatment to the disease prevention so as to achieve the purpose of obtaining healthy body. According to data statistics, more than half of people in China are in the state of chronic fatigue syndrome, and about 70% of people in urban population are occupied. Therefore, it is necessary to provide a medicine or health food effective in relieving fatigue. However, since fatigue itself is not and does not directly cause a serious disease in the relevant population, the necessity or meaning of treatment by a drug is not great from the viewpoint of pharmaceutical economics, and there are some adverse effects of the drug itself more or less. Therefore, in recent years, a health food for relieving fatigue has been attracting attention of researchers. Although there are many health foods for relieving fatigue on the market, these health foods generally have the defects of insignificant effect or single effect. Therefore, it is necessary to develop a safe and effective health food for relieving fatigue.
Disclosure of Invention
Aiming at the technical problems that the existing health care product for relieving fatigue is single in anti-fatigue effect and unobvious, the invention provides the traditional Chinese medicine peptide oral liquid for relieving fatigue and the preparation method thereof.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following raw material components in parts by weight: 15-25 parts of American ginseng, 10-20 parts of rhodiola rosea, 10-15 parts of centella asiatica, 5-10 parts of black mulberry, 4-8 parts of morinda officinalis, 2-6 parts of passion flower, 2-4 parts of peach gum, 5-10 parts of fragrant solomonseal rhizome, 8-12 parts of bighead atractylodes rhizome and 15-30 parts of red date.
Preferably, the traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following raw material components in parts by weight: 16-22 parts of American ginseng, 13-17 parts of rhodiola rosea, 12-14 parts of centella asiatica, 7-9 parts of black mulberry, 5-7 parts of morinda officinalis, 3-6 parts of passion flower, 3-4 parts of peach gum, 6-8 parts of fragrant solomonseal rhizome, 9-11 parts of bighead atractylodes rhizome and 20-28 parts of red date.
Further preferably, the traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following raw material components in parts by weight: 20 parts of American ginseng, 15 parts of rhodiola rosea, 13 parts of centella asiatica, 8 parts of black mulberry, 6 parts of morinda officinalis, 5 parts of passion flower, 3 parts of peach gum, 7 parts of polygonatum odoratum, 10 parts of bighead atractylodes rhizome and 25 parts of red date.
The traditional Chinese medicine peptide oral liquid is also prepared by a preferred preparation method, and the specific preparation method comprises the sequential steps of decocting in water, fermenting lactobacillus rhamnosus, fermenting bifidobacterium longum, sterilizing and filling. Experiments of the inventor find that the oral liquid prepared by fermenting lactobacillus rhamnosus and then fermenting bifidobacterium longum has better anti-fatigue effect, so that the oral liquid has the following advantages: further preferably, the traditional Chinese medicine peptide oral liquid for relieving fatigue is characterized by comprising the steps of fermenting water decoction liquid by lactobacillus rhamnosus and then fermenting by bifidobacterium longum.
The invention also provides a preparation method of the traditional Chinese medicine peptide oral liquid for relieving fatigue, which comprises the following steps:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water, uniformly mixing, and decocting to obtain decoction;
step two, adding the decoction into a fermentation tank, inoculating lactobacillus rhamnosus liquid, and performing primary fermentation to obtain primary fermentation liquid;
inoculating bifidobacterium longum liquid into the primary fermentation liquid, performing secondary fermentation, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue.
According to the preparation method of the traditional Chinese medicine peptide oral liquid for relieving fatigue, provided by the invention, the lactobacillus rhamnosus and the bifidobacterium longum are adopted to ferment each traditional Chinese medicine component, so that the nutrition components and the active components in each traditional Chinese medicine component are fully released, a large amount of active peptide components are generated in the fermentation process, the absorption and the utilization of the active components in the traditional Chinese medicine are facilitated, the intestinal burden of a human body is relieved, and the fatigue relieving effect of the formula is improved; in addition, the lactobacillus rhamnosus and the bifidobacterium longum are fermented for two times, so that the taste of the fermentation liquid can be obviously improved, the prepared traditional Chinese medicine peptide oral liquid has good taste, the compliance of people is improved, and the market prospect is wide.
Preferably, in the first step, the addition amount of the purified water is 8-10 times of the total weight of each raw material.
Preferably, in the first step, the temperature of the decoction is 90-95 ℃ and the decoction time is 6-8h.
The preferred decoction step helps to disrupt the cell wall and promote digestion of intracellular proteins, thereby helping to shorten the subsequent fermentation time.
Preferably, in the second step, the inoculation amount of the lactobacillus rhamnosus liquid is 4% -6% (v/v).
Preferably, in the second step, the temperature of the primary fermentation is 25-30 ℃ and the fermentation time is 1-2d.
Preferably, in the third step, the inoculation amount of the bifidobacterium longum liquid is 2% -3% (v/v).
Preferably, in the third step, the temperature of the secondary fermentation is 25-30 ℃ and the fermentation time is 3-4d.
The lactobacillus rhamnosus activating bacterial liquid and the bifidobacterium longum activating bacterial liquid can be obtained according to a conventional activating method.
According to one specific embodiment of the invention, the lactobacillus rhamnosus liquid can be prepared by the following steps: inoculating the refrigerated lactobacillus rhamnosus to liquid MRS culture medium, and anaerobically culturing at 30-40deg.C for 18-24 hr at 180rpm/min to obtain lactobacillus rhamnosus liquid.
Liquid MRS medium: 10.0g/L casein peptone, 10.0g/L beef extract, 5.0g/L yeast extract, 20.0g/L glucose, 2.0g/L dipotassium hydrogen phosphate, 1.0g/L Tween 80, 2.0g/L tri-ammonium citrate, 5.0g/L sodium acetate, 0.1g/L magnesium sulfate, 0.05g/L manganese sulfate, pH 6.5 and sterilizing at 121 ℃ for 20min.
According to a specific embodiment of the present invention, the bifidobacterium longum liquid may be prepared by the following steps:
inoculating the stored Bifidobacterium longum to BBL agar culture medium, and anaerobic culturing at 35-37deg.C for 72-75 hr at 180rpm/min to obtain Bifidobacterium longum liquid.
BBL agar medium: 15g/L peptone, 20g/L glucose, 2g/L yeast extract, 0.5g/L soluble starch, 5g/L sodium chloride, 0.5 g/LL-cysteine, 5g/L tomato extract, 2g/L liver extract, 20g/L agar, pH7.0; mixing above materials with Tween 80 and distilled water (70 g:1ml:1000 ml), and sterilizing at 115deg.C for 20min.
The recommended dosage of the traditional Chinese medicine peptide oral liquid for relieving fatigue is 60 mL/person/d.
Compared with the prior art, the traditional Chinese medicine peptide oral liquid for relieving fatigue provided by the invention has the advantages that the traditional Chinese medicine peptide oral liquid for relieving fatigue is prepared according to various causes of fatigue, 10 traditional Chinese medicines such as American ginseng, rhodiola rosea, centella asiatica and the like are combined according to specific content, so that the total prescription mainly has the effects of replenishing essence, nourishing yin and blood, replenishing but not greasy, and dredging but not deficiency, thereby achieving the effects of harmonizing yin and yang, tonifying five viscera, and nourishing essence, qi and spirit together, has the effect of relieving fatigue together, and has obvious effects of preventing fatigue syndrome and keeping the human body vigorous and active; the raw materials used in the product provided by the invention are all medicinal and edible raw materials, so that the product is safe and reliable, and is suitable for various crowds, especially sub-healthy human bodies, to take for a long time.
The traditional Chinese medicine composition for relieving fatigue provided by the invention has reasonable formula, combines various medicines, has the effects of tonifying qi and kidney, nourishing yin and blood, replenishing essence and marrow, consolidating primordial qi and the like, can be extracted to obtain more active ingredients through twice fermentation of specific strains, and can be rapidly absorbed and utilized by human bodies, so that the effects of rapidly relieving fatigue, listlessness and weakness are achieved, and the traditional Chinese medicine composition has the advantages of good taste and flavor, convenience in carrying and wide market prospect.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The raw materials used in the examples and comparative examples of the present application were as follows:
american ginseng, rhodiola rosea, centella asiatica, black mulberry, morinda officinalis, passion flower, peach gum, polygonatum, bighead atractylodes rhizome and red date are all produced in China.
| Medicinal material name | Production area |
| American ginseng | Shandong Wendeng |
| Rhodiola root | Xinjiang Altai |
| Centella asiatica | Shanxi Shanlou |
| Black mulberry | Shandong Dezhou |
| Radix Morindae officinalis | Guangdong Deqing |
| Passiflora edulis (Passiflora edulis) | China Guangxi |
| Peach gum | Hubei clock auspicious |
| Polygonatum odoratum (L.) Ohwi | Cangzhou Hebei |
| Atractylodis rhizoma | Zhejiang Xinchang |
| Red date | Cangzhou Hebei |
Lactobacillus rhamnosus fluid and bifidobacterium longum fluid are prepared by the method of the previous examples, wherein the thermostatic shaker selects a vertical thermostatic shaker HNY-1102C.
Example 1
The embodiment provides a traditional Chinese medicine peptide oral liquid for relieving fatigue, which comprises the following raw material components in parts by weight: 20 parts of American ginseng, 15 parts of rhodiola rosea, 13 parts of centella asiatica, 8 parts of black mulberry, 6 parts of morinda officinalis, 5 parts of passion flower, 3 parts of peach gum, 7 parts of polygonatum odoratum, 10 parts of bighead atractylodes rhizome and 25 parts of red date.
The preparation method of the traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following steps:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water with the weight being 9 times of the total weight of the raw materials, uniformly mixing, and decocting at 93 ℃ for 7 hours to obtain decoction;
step two, adding the decoction into a fermentation tank, inoculating lactobacillus rhamnosus liquid, wherein the inoculum size of the lactobacillus rhamnosus liquid is 5% (v/v), and the thallus concentration of the lactobacillus rhamnosus after inoculation is 1.5X10% 7 cfu/mL, fermenting at 28 ℃ for 1.5d to obtain primary fermentation liquor;
step three, inoculating Bifidobacterium longum liquid into the primary fermentation liquid, wherein the inoculum size of the Bifidobacterium longum liquid is 2.5% (v/v), and the thallus concentration of the Bifidobacterium longum after inoculation is 1.2X10% 7 cfu/mL, fermenting at 28 ℃ for 3.5d, filtering, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue.
Example 2
The embodiment provides a traditional Chinese medicine peptide oral liquid for relieving fatigue, which comprises the following raw material components in parts by weight: 15 parts of American ginseng, 20 parts of rhodiola rosea, 10 parts of centella asiatica, 5 parts of black mulberry, 8 parts of morinda officinalis, 2 parts of passion flower, 4 parts of peach gum, 10 parts of polygonatum odoratum, 8 parts of bighead atractylodes rhizome and 30 parts of red date.
The preparation method of the traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following steps:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water with an amount which is 8 times of the total weight of the raw materials, uniformly mixing, and decocting at 90 ℃ for 8 hours to obtain decoction;
step two, adding the decoction into a fermentation tank, inoculating lactobacillus rhamnosus liquid, wherein the inoculum size of the lactobacillus rhamnosus liquid is 4% (v/v), and the thallus concentration of the lactobacillus rhamnosus after inoculation is 1.6X10% 6 cfu/mL, fermenting at 25 ℃ for 2d to obtain primary fermentation liquor;
step three, inoculating Bifidobacterium longum liquid into the primary fermentation liquid, wherein the inoculum size of the Bifidobacterium longum liquid is 3% (v/v), and the thallus concentration of the Bifidobacterium longum after inoculation is 2.0X10% 7 cfu/mL, fermenting at 25 ℃ for 4d, filtering, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue.
Example 3
The embodiment provides a traditional Chinese medicine peptide oral liquid for relieving fatigue, which comprises the following raw material components in parts by weight: 25 parts of American ginseng, 10 parts of rhodiola rosea, 15 parts of centella asiatica, 10 parts of black mulberry, 4 parts of morinda officinalis, 6 parts of passion flower, 2 parts of peach gum, 5 parts of polygonatum odoratum, 12 parts of bighead atractylodes rhizome and 15 parts of red date.
The preparation method of the traditional Chinese medicine peptide oral liquid for relieving fatigue comprises the following steps:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water with the weight being 10 times of the total weight of the raw materials, uniformly mixing, and decocting at 95 ℃ for 6 hours to obtain decoction;
step two, adding the decoction into a fermentation tank, inoculating lactobacillus rhamnosus liquid, wherein the inoculum size of the lactobacillus rhamnosus liquid is 6% (v/v), and the thallus concentration of the lactobacillus rhamnosus after inoculation is 1.3X10% 7 cfu/mL, fermenting at 30 ℃ for 1d to obtain primary fermentation liquor;
step three, inoculating Bifidobacterium longum liquid with the inoculum size of 2% (v/v) into the primary fermentation liquid, and inoculatingThe cell concentration of the post-longum bifidobacterium was 2.5X10 6 cfu/mL, fermenting at 30 ℃ for 3d, filtering, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue.
The lactobacillus rhamnosus solution in the above examples 1-3 can be prepared by the following steps: inoculating the refrigerated lactobacillus rhamnosus to a liquid MRS culture medium, and performing anaerobic culture at a constant temperature of 180rpm/min and a temperature of 35 ℃ for 24 hours to obtain lactobacillus rhamnosus liquid.
Liquid MRS medium: 10.0g/L casein peptone, 10.0g/L beef extract, 5.0g/L yeast extract, 20.0g/L glucose, 2.0g/L dipotassium hydrogen phosphate, 1.0g/L Tween 80, 2.0g/L tri-ammonium citrate, 5.0g/L sodium acetate, 0.1g/L magnesium sulfate, 0.05g/L manganese sulfate, pH 6.5 and sterilizing at 121 ℃ for 20min.
The bifidobacterium longum liquid can be prepared by the following steps:
the bifidobacterium longum liquid can be prepared by the following steps:
inoculating the stored Bifidobacterium longum to BBL agar culture medium, and anaerobic culturing at 35-37deg.C for 72-75 hr at 180rpm/min to obtain Bifidobacterium longum liquid.
BBL agar medium: 15g/L peptone, 20g/L glucose, 2g/L yeast extract, 0.5g/L soluble starch, 5g/L sodium chloride, 0.5 g/LL-cysteine, 5g/L tomato extract, 2g/L liver extract, 20g/L agar, pH7.0; mixing the above materials with Tween 80 and distilled water (wherein the ratio of materials is 70g:1ml:1000 ml), and sterilizing at 115deg.C for 20min.
Comparative example 1
The comparative example provides a traditional Chinese medicine peptide oral liquid for relieving fatigue, the raw material proportion and the preparation method are the same as those of the example 1, except that centella asiatica in the example 1 is replaced by the same amount of rhodiola rosea, and the bighead atractylodes rhizome is replaced by the same amount of American ginseng, and the specific components are as follows:
30 parts of American ginseng, 28 parts of rhodiola rosea, 8 parts of black mulberry, 6 parts of morinda officinalis, 5 parts of passion flower, 3 parts of peach gum, 7 parts of fragrant solomonseal rhizome and 25 parts of red date.
The preparation process was exactly the same as in example 1.
Comparative example 2
The comparative example provides a traditional Chinese medicine peptide oral liquid for relieving fatigue, the raw material proportion is the same as that of the example 1, the preparation method in the example 1 is replaced by lactobacillus rhamnosus and bifidobacterium longum for simultaneous fermentation, and the specific steps are as follows:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water with the weight being 9 times of the total weight of the raw materials, uniformly mixing, and decocting at 93 ℃ for 7 hours to obtain decoction;
step two, adding the decoction into a fermentation tank, and inoculating lactobacillus rhamnosus liquid and bifidobacterium longum; wherein the inoculum size of the lactobacillus rhamnosus liquid is 5% (v/v), the inoculum size of the bifidobacterium longum liquid is 2.5% (v/v), fermenting for 5d at 28 ℃, filtering, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue.
Fatigue relieving function test
Test object:
the test subjects were 15 month-old Kunming mice, each half of a male and female, and were divided into example 1 group, comparative example 2 group and negative control group, wherein the example 1 group, comparative example 1 group and comparative example 2 group were respectively divided into 3 test groups, one test group was a weight-bearing swimming test, the second test group was a serum urea test, and the third test group was a liver glycogen test. Each test group had 10 mice, and the example 1 group had three dose groups (1 low dose group, 1 medium dose group, 1 high dose group).
Test sample:
the products prepared in the embodiment 1 are designed into a low dose group (0.42 mL/Kg), a medium dose group (0.83 mL/Kg) and a high dose group (2.50 mL/Kg), the doses of the products are 5 times, 10 times and 30 times of the recommended amount of a human body respectively, and the products are subjected to a gastric lavage method once a day; comparative example 1 uses the product of comparative example 1 of the present invention, comparative example 2 uses the product of comparative example 2 of the present invention, and the dosages of the comparative example 1 and the comparative example 2 are: low dose (0.42 mL/Kg), medium dose (0.83 mL/Kg) and high dose (2.50 mL/Kg); the negative control group was given an equal volume of distilled water and was continuously gavaged for 30 days.
Test method and results:
1. weight bearing swimming test
After the last administration of the test object for 30min, the mice were put in a swimming box to swim with a water depth of about 35cm and a water temperature (25 soil 1) DEG C, and the roots of the rat tails were loaded with 5% of the weight of the lead skin. The time from the start of the swimming to death of the mice was recorded as the swimming time (min) of the mice. And the body weight of the mice was measured before the start of the test and after the end of the test.
TABLE 1
| Group of | Initial body weight (g) | Ending weight (g) | Swimming time (min) |
| Negative control group | 19.3±1.2 | 40.5±1.8 | 19.52±4.85 |
| Example 1 Low dose group | 19.5±0.9 | 41.3±2.1 | 23.15±6.27 |
| Dose group in example 1 | 19.2±1.1 | 40.7±1.9 | 33.67±5.84 * |
| Example 1 high dose group | 19.4±1.0 | 41.2±2.5 | 36.23±6.51 * |
| Comparative example 1 Low dose group | 19.1±1.0 | 41.1±2.0 | 22.35±5.12 |
| Dose group in comparative example 1 | 19.2±0.9 | 41.6±1.5 | 26.35±4.27 |
| Comparative example 1 high dose group | 19.0±1.2 | 40.7±1.9 | 27.27±4.27 |
| Comparative example 2 Low dose group | 19.2±1.4 | 41.4±1.2 | 24.75±3.61 |
| Dose group in comparative example 2 | 19.3±0.8 | 41.3±0.9 | 28.75±3.61 |
| Comparative example 2 high dose group | 19.4±0.9 | 40.8±1.1 | 31.75±5.31 * |
Note that: ratio to blank, x represents P <0.05.
From the above results, it can be seen that the mice in each dose group have no significant difference (P > 0.05) in weight and weight gain at each period compared with the negative control group; the mean swimming time of the mice in each dose group was prolonged compared with that of the negative control group, and the medium dose group and the high dose group of example 1 were significantly different from those of the negative control group (P < 0.05). The statistical results of the combination of example 1, comparative example 1 and comparative example 2 show that the sample of example 1 has an effect of significantly prolonging the load swimming time of mice compared with other samples.
2. Serum urea nitrogen and lactic acid content determination
After the last administration for 30min, the mice swim for 90min in water with the temperature of 30 ℃ without load, draw out eyeballs for blood sampling after resting for 60min, place the eyeballs in a refrigerator with the temperature of 4 ℃ for 3h, centrifuge for 15min at 2000r/min after blood coagulation, and take serum to measure urea nitrogen.
TABLE 2
Note that: * P<0.05, with a significant difference compared to the blank group; ** P<0.01, with a very significant difference compared to the blank.
From the above results, it can be seen that the mice in each dose group had no significant difference (P > 0.05) in weight and weight gain at each stage compared with the negative control group. Serum urea levels of mice in the medium and high dose groups were very significantly different (P < 0.01) compared to the negative control group. The statistical results of the combination of example 1, comparative example 1 and comparative example 2 show that the samples of the examples can delay the occurrence of fatigue or accelerate the rapid elimination of fatigue.
3. Determination of liver glycogen
After the last administration for 30min, the mice are sacrificed, the livers are rinsed by normal saline and then sucked by filter paper, tissue homogenate is taken, centrifugation is carried out for 15min at 3000r/min, supernatant is taken, and the content of liver glycogen is measured.
TABLE 3 Table 3
| Group of | Initial weight (g) | Final weight (g) | Liver glycogen (mg/g) |
| Negative control group | 19.7±0.7 | 40.5±1.9 | 17.63±3.95 |
| Example 1 Low dose group | 20.4±1.2 | 39.5±2.3 | 19.12±4.23 |
| Dose group in example 1 | 19.9±1.3 | 40.3±2.2 | 25.83±3.51 ** |
| Example 1 high dose group | 20.2±1.1 | 39.9±2.4 | 26.12±2.76 ** |
| Comparative example 1 Low dose group | 20.0±1.3 | 40.9±2.3 | 18.42±4.11 |
| Dose group in comparative example 1 | 19.8±1.0 | 41.2±1.4 | 19.02±4.13 |
| Comparative example 1 high dose group | 20.1±0.9 | 40.8±1.9 | 20.34±3.75 |
| Comparative example 2 Low dose group | 20.5±1.3 | 39.7±2.2 | 18.97±5.10 |
| Dose group in comparative example 2 | 19.8±1.2 | 41.0±1.7 | 20.52±4.23 |
| Comparative example 2 high dose group | 21.2±1.4 | 40.5±2.1 | 22.18±3.73 * |
Note that: * P<0.05, with a significant difference compared to the blank group; ** P<0.01, with a very significant difference compared to the blank.
From the above results, it can be seen that the mice in each dose group have no significant difference (P > 0.05) in weight and weight gain at each period compared with the negative control group; the liver glycogen of the mice in each dosage group is higher than that in the negative control group, and compared with the negative control group of the liver glycogen content of the mice in the high and medium dosage groups, the liver glycogen content of the mice in the high and medium dosage groups has extremely significant difference (P < 0.01). The statistical results of the combination of example 1, comparative example 1 and comparative example 2 show that the samples of the examples can increase the liver glycogen storage of mice, improve the endurance of the mice and relieve the occurrence of physical fatigue.
In conclusion, the traditional Chinese medicine peptide oral liquid provided by the invention has obvious anti-fatigue efficacy for sub-healthy people with low immunity, easy fatigue and weak and multiple diseases; the food preservative is not used in the product, and the raw materials are all medicinal and edible raw materials, so that the food is safe and reliable and is suitable for long-term eating.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, or alternatives falling within the spirit and principles of the invention.
Claims (5)
1. A traditional Chinese medicine peptide oral liquid for relieving fatigue is characterized by being prepared from the following raw material components in parts by weight: 15-25 parts of American ginseng, 10-20 parts of rhodiola rosea, 10-15 parts of centella asiatica, 5-10 parts of black mulberry, 4-8 parts of morinda officinalis, 2-6 parts of passion fruit, 2-4 parts of peach gum, 5-10 parts of fragrant solomonseal rhizome, 8-12 parts of bighead atractylodes rhizome and 15-30 parts of red date;
the preparation method of the traditional Chinese medicine peptide oral liquid comprises the following steps:
firstly, weighing raw material components according to a designed proportion, crushing the weighed raw materials, adding the crushed raw materials into purified water, uniformly mixing, and decocting to obtain decoction;
step two, adding the decoction into a fermentation tank, inoculating lactobacillus rhamnosus liquid, and performing primary fermentation to obtain primary fermentation liquid;
inoculating bifidobacterium longum liquid into the primary fermentation liquid, performing secondary fermentation, sterilizing and filling to obtain the traditional Chinese medicine peptide oral liquid for relieving fatigue;
wherein in the first step, the adding amount of the purified water is 8-10 times of the total weight of the raw materials, the decocting temperature is 90-95 ℃ and the decocting time is 6-8h;
in the second step, the temperature of the primary fermentation is 25-30 ℃ and the fermentation time is 1-2d;
in the third step, the temperature of the secondary fermentation is 25-30 ℃ and the fermentation time is 3-4d.
2. The traditional Chinese medicine peptide oral liquid for relieving fatigue according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 16-22 parts of American ginseng, 13-17 parts of rhodiola rosea, 12-14 parts of centella asiatica, 7-9 parts of black mulberry, 5-7 parts of morinda officinalis, 3-6 parts of passion flower, 3-4 parts of peach gum, 6-8 parts of fragrant solomonseal rhizome, 9-11 parts of bighead atractylodes rhizome and 20-28 parts of red date.
3. The traditional Chinese medicine peptide oral liquid for relieving fatigue according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 20 parts of American ginseng, 15 parts of rhodiola rosea, 13 parts of centella asiatica, 8 parts of black mulberry, 6 parts of morinda officinalis, 5 parts of passion flower, 3 parts of peach gum, 7 parts of polygonatum odoratum, 10 parts of bighead atractylodes rhizome and 25 parts of red date.
4. The oral liquid according to any one of claims 1 to 3, wherein in the second step, the lactobacillus rhamnosus activating bacteria solution is inoculated in an amount of 4% -6% (v/v).
5. The oral liquid according to any one of claims 1 to 3, wherein in the third step, the inoculation amount of the bifidobacterium longum activating bacteria liquid is 2% -3% (v/v).
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757442A (en) * | 2010-03-19 | 2010-06-30 | 磐安县诸源中药材科技开发有限公司 | Anti-fatigue Chinese medicinal composition and lagehead atractylodes paste |
| CN102451260A (en) * | 2010-10-21 | 2012-05-16 | 海南医学院 | Traditional Chinese medicine composition with function of alleviating fatigue and preparation method thereof |
| CN106309506A (en) * | 2016-09-23 | 2017-01-11 | 泰安大凡神农制药有限公司 | Probiotic composition with menstruation regulating function and application thereof |
| CN109717340A (en) * | 2019-03-01 | 2019-05-07 | 江南大学 | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101757442A (en) * | 2010-03-19 | 2010-06-30 | 磐安县诸源中药材科技开发有限公司 | Anti-fatigue Chinese medicinal composition and lagehead atractylodes paste |
| CN102451260A (en) * | 2010-10-21 | 2012-05-16 | 海南医学院 | Traditional Chinese medicine composition with function of alleviating fatigue and preparation method thereof |
| CN106309506A (en) * | 2016-09-23 | 2017-01-11 | 泰安大凡神农制药有限公司 | Probiotic composition with menstruation regulating function and application thereof |
| CN109717340A (en) * | 2019-03-01 | 2019-05-07 | 江南大学 | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis |
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