CN115669489B - Regulator for promoting effective accumulation of curculigoside and regulating method - Google Patents
Regulator for promoting effective accumulation of curculigoside and regulating method Download PDFInfo
- Publication number
- CN115669489B CN115669489B CN202211716441.2A CN202211716441A CN115669489B CN 115669489 B CN115669489 B CN 115669489B CN 202211716441 A CN202211716441 A CN 202211716441A CN 115669489 B CN115669489 B CN 115669489B
- Authority
- CN
- China
- Prior art keywords
- regulator
- curculigo orchioides
- roots
- irrigating
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- WUOIMLIHAHOCCR-UHFFFAOYSA-N curculigoside Natural products COc1ccc(OC2OC(CO)C(O)C(O)C2O)c(OC)c1C(=O)OCc3cc(O)ccc3OC4OC(CO)C(O)C(O)C4O WUOIMLIHAHOCCR-UHFFFAOYSA-N 0.000 title claims abstract description 36
- SJJRKHVKAXVFJQ-UHFFFAOYSA-N curculigoside I Natural products COC1=CC=CC(OC)=C1C(=O)OCC1=CC(O)=CC=C1OC1C(O)C(O)C(O)C(CO)O1 SJJRKHVKAXVFJQ-UHFFFAOYSA-N 0.000 title claims abstract description 36
- SJJRKHVKAXVFJQ-QKYBYQKWSA-N curculigoside a Chemical compound COC1=CC=CC(OC)=C1C(=O)OCC1=CC(O)=CC=C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 SJJRKHVKAXVFJQ-QKYBYQKWSA-N 0.000 title claims abstract description 36
- 238000009825 accumulation Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 230000001105 regulatory effect Effects 0.000 title abstract description 7
- 244000084791 Curculigo orchioides Species 0.000 claims abstract description 71
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 52
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims abstract description 50
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 26
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000012010 growth Effects 0.000 claims abstract description 20
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 19
- 235000004279 alanine Nutrition 0.000 claims abstract description 19
- 239000003245 coal Substances 0.000 claims abstract description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 25
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 16
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 16
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 12
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 12
- 229960001763 zinc sulfate Drugs 0.000 claims description 12
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 11
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims description 11
- 230000033228 biological regulation Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 20
- 229930000044 secondary metabolite Natural products 0.000 abstract description 9
- 239000005648 plant growth regulator Substances 0.000 abstract description 6
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 abstract description 2
- 229910001431 copper ion Inorganic materials 0.000 abstract description 2
- 230000002262 irrigation Effects 0.000 description 17
- 238000003973 irrigation Methods 0.000 description 17
- 241000196324 Embryophyta Species 0.000 description 16
- 230000035882 stress Effects 0.000 description 12
- 230000000638 stimulation Effects 0.000 description 11
- 239000003513 alkali Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 239000005712 elicitor Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 7
- 229910001385 heavy metal Inorganic materials 0.000 description 7
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 230000008641 drought stress Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 229930003944 flavone Natural products 0.000 description 5
- 150000002212 flavone derivatives Chemical class 0.000 description 5
- 235000011949 flavones Nutrition 0.000 description 5
- 150000008442 polyphenolic compounds Chemical class 0.000 description 5
- 235000013824 polyphenols Nutrition 0.000 description 5
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- XGVJWXAYKUHDOO-UHFFFAOYSA-N galanthidine Natural products C1CN2CC3=CC=4OCOC=4C=C3C3C2C1=CC(O)C3O XGVJWXAYKUHDOO-UHFFFAOYSA-N 0.000 description 4
- XGVJWXAYKUHDOO-DANNLKNASA-N lycorine Chemical compound C1CN2CC3=CC=4OCOC=4C=C3[C@H]3[C@H]2C1=C[C@H](O)[C@H]3O XGVJWXAYKUHDOO-DANNLKNASA-N 0.000 description 4
- KQAOMBGKIWRWNA-UHFFFAOYSA-N lycorine Natural products OC1C=C2CCN3C2C(C1O)c4cc5OCOc5cc34 KQAOMBGKIWRWNA-UHFFFAOYSA-N 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- PWVXXGRKLHYWKM-UHFFFAOYSA-N 5-[2-(benzenesulfonyl)ethyl]-3-[(1-methylpyrrolidin-2-yl)methyl]-1h-indole Chemical compound CN1CCCC1CC(C1=C2)=CNC1=CC=C2CCS(=O)(=O)C1=CC=CC=C1 PWVXXGRKLHYWKM-UHFFFAOYSA-N 0.000 description 3
- 241001633628 Lycoris Species 0.000 description 3
- 230000003042 antagnostic effect Effects 0.000 description 3
- 238000007405 data analysis Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241000234276 Curculigo Species 0.000 description 2
- 241000721047 Danaus plexippus Species 0.000 description 2
- 102000018997 Growth Hormone Human genes 0.000 description 2
- 108010051696 Growth Hormone Proteins 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 150000002338 glycosides Chemical class 0.000 description 2
- 239000000122 growth hormone Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 229930000223 plant secondary metabolite Natural products 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 description 1
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 241000234270 Amaryllidaceae Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 206010021118 Hypotonia Diseases 0.000 description 1
- 239000005985 Paclobutrazol Substances 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000031975 Yang Deficiency Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229960000355 copper sulfate Drugs 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000009867 er-xian decoction Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 229960001781 ferrous sulfate Drugs 0.000 description 1
- 208000017561 flaccidity Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- -1 meJA Chemical compound 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000000920 spermatogeneic effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Images
Abstract
The invention relates to the technical field of traditional Chinese medicinal material planting, and particularly discloses a regulator for promoting effective accumulation of curculigoside and a regulating method, wherein the regulating method comprises the following steps: (1) Irrigating the roots of the transplanted curculigo orchioides seedlings by using a regulator A at the 6 th month of the next year in the initial growth stage of the curculigo orchioides, and irrigating the roots by using a regulator B after 7-10 days; (2) Irrigating roots of the Curculigo orchioides in the initial full growth period of 8 months with the regulator C, and irrigating roots of the Curculigo orchioides with the regulator D after 7-10 days; (3) Irrigating the roots of the curculigo orchioides in the last growth stage of 10 months by using the regulator E, and irrigating the roots once again by using the regulator E after 7-10 days; (4) repeating the steps (1) to (3) every year until the coal is mined; according to the invention, the two plant growth regulators of abscisic acid and salicylic acid with appropriate concentrations, copper ions and zinc ions are adopted to stimulate the curculigo orchioides to generate secondary metabolites, and the alanine is matched to greatly promote the effective accumulation of the curculigo orchioides, so that the effective accumulation of the curculigo orchioides can be improved to more than 1.6 times every year.
Description
Technical Field
The invention relates to the technical field of planting of traditional Chinese medicinal materials, in particular to a regulator for promoting effective accumulation of curculigoside and a regulating method, which induce secondary metabolism accumulation in the planting process of the traditional Chinese medicinal materials.
Background
Curculigoorchis orchioides (Curculigoorchioides des Gaertn.) is a perennial herb of Curculigoorchis of Amaryllidaceae, has the effects of invigorating kidney yang, strengthening tendons and bones and dispelling cold-dampness by using underground rhizomes as a medicine, is mainly used for treating impotence and cold sperm, flaccidity of bones and muscles, cold pain of waist and knees, yang deficiency and cold diarrhea and other symptoms, is a health product represented by jin wine and spermatogenic capsules and a monarch medicine of a Chinese patent medicine, and is also a monarch medicine of famous classics 'Erxian decoction'.
The content of curculigoside is the most important standard of quality standard of curculigoside, wherein the standard is 0.1% in '2020 edition of Chinese pharmacopoeia', and the enterprise standard of many pharmaceutical enterprises reaches more than 0.15%. Meanwhile, the price of the curculigoside is greatly increased along with the increase of the content of the curculigoside. The curculigo orchioides in the current market is mainly divided into imported curculigo orchioides in south-east Asia countries, curculigo orchioides produced in Yunnan countries and curculigo orchioides produced in Sichuan countries, the market judges that the curculigoside content of the curculigo orchioides produced in Sichuan is generally the highest according to historical experience, the curculigoside produced in Sichuan is the second highest in Yunnan countries and the imported curculigo orchioides produced in Yunnan countries hardly meet pharmacopeia standards, so that the current market price is the highest in Yibin in Sichuan and the lowest in each production area in Yunnan countries, and the market price in Sichuan is 5-6 times of the imported product price from the current market, so that the curculigoside content in the production areas in Yunnan is increased, the pharmacopeia standards are met, and the significance in meeting market demands is achieved.
The generation of secondary metabolites of natural plants is influenced by environmental factors, such as drought, water accumulation, saline-alkali, heavy metals, illumination intensity, illumination duration and the like, while different plants, different organs of the same plant and different secondary metabolites may produce different results under different environmental factors. In the process of planting medicinal plants, elicitors are often adopted to induce the generation of plant secondary metabolites, and different elicitors, induction concentrations, action times, action parts and the like have specificity on different plants, different organs and different target substances, and are supposed to be related to the expression regulation and control of related enzyme genes. Currently, elicitors are mainly classified into microbial elicitors such as fungal spores, mycelia, homogenate, fungal culture solution and the like, abiotic elicitors such as physical elicitors such as temperature, drought, rays and the like, chemical elicitors such as heavy metal ions of copper, cadmium, zinc, iron, silver and the like, amino acids and the like, plant growth regulators such as ABA, meJA, SA, GA3, BR, CCC and the like. Different elicitors may also have interaction, may produce synergistic or antagonistic effects, or may not interact with each other.
In the plant planting process, because environmental factors have uncertainty and uncontrollable conditions, the biological inductor is often not used as a method for promoting the accumulation of secondary metabolites. In the heavy metal aspect, copper, iron, zinc and the like are often used as inducers, and other heavy metals such as cadmium, lead, nickel, cobalt and the like are often used as important detection indexes for whether the heavy metals exceed the standard or not in medicinal materials and foods, so the application is relatively less.
At present, the research on the correlation between the curculigo orchioides and curculigo orchioides is mainly focused on the research on curculigo orchioides in various production places, the loss of the curculigo orchioides is reduced by utilizing an optimal processing method, and the like, and no relevant documents are reported on the aspect of promoting the accumulation of the curculigo orchioides content in the planting process by an artificial mode.
Disclosure of Invention
The invention mainly aims to provide a regulator for promoting the effective accumulation of curculigoside and a regulating method thereof, so as to solve the problem of low content of curculigoside in a curculigoside medicinal material.
In order to achieve the above purpose, the invention provides the following technical scheme:
a regulation method for promoting effective accumulation of curculigoside comprises the following steps:
(1) Irrigating the roots of the transplanted curculigo orchioides seedlings by using a regulator A at the 6 th month of the next year in the initial growth stage of the curculigo orchioides, and irrigating the roots by using a regulator B after 7-10 days;
(2) Irrigating roots of the curculigo orchioides in the initial full growth period of 8 months by using a regulator C, and irrigating roots of the curculigo orchioides by using a regulator D after 7-10 days;
(3) Irrigating the roots of the curculigo orchioides in the later growth period of 10 months, and irrigating the roots of the curculigo orchioides once again by using the regulator E after 7-10 days;
(4) Repeating the steps (1) to (3) every year until the coal is mined;
the components of the regulators A, B, C, D and E comprise plant growth hormone and amino acid; the plant growth hormone comprises 5-20 mg/L of abscisic acid, 80-250 mg/L of naphthylacetic acid, 40-120 mg/L of salicylic acid or 0.1-0.3 mg/L of brassinolide, and the amino acid is alanine with the dosage of 2.0-3.0 g/L.
Further, the regulator A in the step (1) consists of 5-10 mg/L of abscisic acid, 2.0-3.0 g/L of alanine and 3-5 mg/L of copper sulfate.
Further, the regulator B in the step (1) consists of 80-120 mg/L of naphthylacetic acid, 40-60 mg/L of salicylic acid and 0.1-0.2 mg/L of brassinolide.
Further, the regulator C in the step (2) consists of 15-20 mg/L of abscisic acid, 2.0-3.0 g/L of alanine, 3-5 mg/L of copper sulfate and 10-15 mg/L of zinc sulfate.
Further, the regulator D in the step (2) consists of 200-250 mg/L of naphthylacetic acid, 80-100 mg/L of salicylic acid and 0.2-0.3 mg/L of brassinolide.
Further, the regulator E in the step (3) consists of 200-250 mg/L of naphthylacetic acid, 100-120 mg/L of salicylic acid, 2.0-3.0 g/L of alanine, 1-2 mg/L of copper sulfate and 25-30 mg/L of zinc sulfate.
Preferably, the dosage of the regulators A, B, C, D and E in the steps (1) to (3) is 200-250 kg per mu.
The invention has the beneficial effects that:
1. the curculigoside belongs to a secondary metabolite, the secondary metabolite is generated by adopting two plant growth regulators of abscisic acid and salicylic acid with proper concentration and copper ions and zinc ions to stimulate the curculigoside, and the effective accumulation of the curculigoside can be greatly promoted by matching alanine, so that the effective accumulation of the curculigoside can be improved to more than 1.6 times every year.
2. The invention can also promote the accumulation of two active ingredients of total polyphenol and total flavone.
3. The invention can not promote accumulation of toxic substance lycorine in the curculigo orchioides while promoting accumulation of curculigo orchioides glycoside, total polyphenol and total flavone.
4. Generally, when secondary metabolites are accumulated, the biomass reduction of nutritive organs of the curculigo orchioides is influenced, particularly, abscisic acid and salicylic acid are used under manual intervention, however, in the implementation process of the method, ABA is used for regulating and controlling the expression of related genes accumulated by curculigo orchioides, and NAA and BR are used for treating the curculigo orchioides for 7-10 days later, so that the influence of ABA on the growth of the curculigo orchioides is eliminated in time, and the yield which is influenced by the ABA is not influenced.
5. In the invention, naphthylacetic acid and brassinolide with proper concentrations are added at the terminal stage of growth of the curculigo orchioides, so that the curculigo orchioides which should be poured out in the last ten days of October to the last ten days of November is delayed to be poured out in the middle ten days of November to the middle ten days of December, the growth time of the curculigo orchioides is prolonged, on one hand, the growth time of a vegetative organ is prolonged, the accumulation of the biomass of each plant of the curculigo orchioides medicinal material is further improved, the yield of the medicinal material is finally improved, on the other hand, the accumulation time of the content of the curculigoside is further increased, and the content of the curculigoside can also be improved to a certain degree.
6. Drought stress, saline-alkali stress and temperature difference stimulation can promote the effective accumulation of the curculigoside, but the three stress modes have high artificial intervention cost, large difficulty in executing quantitative indexes and the like, simultaneously, the drought stress and the saline-alkali stress can also influence the yield of medicinal materials, and the saline-alkali stress can also destroy the ecological environment to a certain extent.
The plant secondary metabolite does not directly participate in the growth and development process of the plant, but plays an important role in improving the adaptability of the plant to the physical environment and the interspecific competitive power, resisting the attack of natural enemies, enhancing the disease resistance and the like, and most of the active ingredients of medicinal plants are derived from the secondary metabolite, namely the curculigoside in the invention, namely the secondary metabolite belonging to the curculigo orchioides.
Drawings
FIG. 1 is a field diagram of a planting test of the present invention;
FIG. 2 is a field diagram of biomass measurement of Curculigo orchioides medicinal material in example 1;
FIG. 3 is a field diagram of biomass measurement of Curculigo orchioides medicinal material in comparative example 1.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the application examples are only for the understanding of the present invention and should not be construed as the specific limitation of the present invention. Those not specifically mentioned herein are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
1. Transplanting the domesticated curculigo orchioides tissue culture seedlings under a photovoltaic panel of a curculigo orchioides planting area in Lincangyun county, and irrigating roots with a regulator A consisting of 5mg/L abscisic acid, 2.0g/L alanine and 3mg/L copper sulfate in ten o' clock before the next year and 6 months after transplantation, wherein the root irrigation amount per mu is 200kg.
2. After 7 days, before ten am, irrigating roots with regulator B composed of 80mg/L naphthylacetic acid, 40mg/L salicylic acid and 0.1mg/L brassinolide, wherein the irrigation amount per mu is 200kg.
3. At the beginning of 8 months, before ten am, the root is irrigated with a regulator C consisting of 15mg/L abscisic acid, 2.0g/L alanine, 3mg/L copper sulfate and 10mg/L zinc sulfate, and the root irrigation amount per mu is 200kg.
4. After 7 days, before ten am, the root is irrigated with a regulator D consisting of 200mg/L of naphthylacetic acid, 80mg/L of salicylic acid and 0.2mg/L of brassinolide, wherein the root irrigation amount is 200kg.
5. In 10 middle of the month, before ten am, the roots are irrigated with a regulator E consisting of 200mg/L of naphthylacetic acid, 100mg/L of salicylic acid, 2.0g/L of alanine, 1mg/L of copper sulfate and 25mg/L of zinc sulfate, and the root irrigation amount per mu is 200kg.
6. After 7 days, before ten am, the root is irrigated once again by using a regulator E consisting of 200mg/L naphthylacetic acid, 100mg/L salicylic acid, 2.0g/L alanine, 1mg/L copper sulfate and 25mg/L zinc sulfate, and the root irrigation amount per mu is 200kg.
7. Repeating the steps 1-6 every year after transplanting.
8. After 12 months in the third year after transplanting, collecting and digging a medicinal material of the curculigo orchioides (containing fibrous roots), detecting the content of the curculigo orchioides glycoside according to a content detection item under 2020 edition of Chinese pharmacopoeia, detecting total flavone and total polyphenol according to a college of Jiangxi traditional Chinese medicine (Guxian tablet quality standard research [ J ] in Jiangxi, 2006, 18 (2): 39-40) published by Yanjun and the like, and detecting the content of lycorine according to a high performance liquid chromatography fingerprint identification column under the item of lycoris serving as a medicinal material in the eighth phase of hong Kong traditional Chinese medicinal material standard.
Example 2
1. The domesticated common curculigo rhizome tissue culture seedlings are transplanted under a photovoltaic panel in a common curculigo rhizome planting area in Lincang Yun county, and the roots are irrigated with a regulator A consisting of 10mg/L abscisic acid, 3.0g/L alanine and 5mg/L copper sulfate in the middle of 6 months of the next year after the transplantation, and the root irrigation amount per mu is 250kg.
2. After 10 days, after five hours in the afternoon, the root is irrigated with a regulator B consisting of 120mg/L naphthylacetic acid, 60mg/L salicylic acid and 0.2mg/L brassinolide, and the irrigation quantity per mu is 250kg.
3. In 8 middle of the month, after five pm, the root is irrigated with a regulator C consisting of 20mg/L abscisic acid, 3.0g/L alanine, 5mg/L copper sulfate and 15mg/L zinc sulfate, and the root irrigation amount per mu is 250kg.
4. After 10 days, after five hours in the afternoon, the root is irrigated with a regulator D consisting of 250mg/L of naphthylacetic acid, 100mg/L of salicylic acid and 0.3mg/L of brassinolide, and the root irrigation amount per mu is 250kg.
5. In 10 Yueyuexing hours, after the afternoon, the root is irrigated with a regulator E consisting of 250mg/L of naphthylacetic acid, 120mg/L of salicylic acid, 3.0g/L of alanine, 2mg/L of copper sulfate and 30mg/L of zinc sulfate, and the root irrigation amount per mu is 250kg.
6. After 10 days, after five hours in the afternoon, the root is irrigated once again by using a regulator E consisting of 50mg/L naphthylacetic acid, 120mg/L salicylic acid, 3.0g/L alanine, 2mg/L copper sulfate and 30mg/L zinc sulfate, and the dosage of irrigating the root per mu is 250kg.
7. Repeating the steps 1-6 every year after transplanting.
8. After 12 months of seedling in the third year after transplanting, the medicinal material of the curculigo orchioides (containing fibrous roots) is picked and dug, the content of the curculigo orchioides is detected according to content detection items under the 2020 edition of Chinese pharmacopoeia, the total flavone and the total polyphenol are detected according to the statement of Jiangxi college of traditional Chinese medicine (quality standard research of the GUXIAN tablets [ J ] 2006, 18 (2): 39-40) published by the Yangjun et al, and the lycorine content is detected according to a high performance liquid chromatography fingerprint identification column under the lycoris item of the medicinal material in the eighth phase of hong Kong Chinese medicinal material standard.
Comparative example 1
1. And transplanting the domesticated curculigo orchioides tissue culture seedlings under a photovoltaic panel in Lincang county, and managing according to the conventional method.
2. After seedling falling in the third year after transplanting, collecting and digging a medicinal material of the curculigo orchioides (containing fibrous roots) in 12 months, detecting the content of the curculigoside according to content detection items under the 2020 edition of Chinese pharmacopoeia, detecting the total flavone and the total polyphenol according to the statement of Jiangxi Chinese medicine institute in the Guxian et al (quality standard research of the GUXIAN tablets [ J ] 2006, 18 (2): 39-40), and detecting the content of the lycorine according to a high performance liquid chromatography fingerprint identification spectrum column under the lycoris item of the medicinal material in the eighth phase of hong Kong Chinese medicinal material standard.
Experimental study
Test site: a planting area of the curculigo orchioides in Lincangyun county;
the method for detecting the curculigoside comprises the following steps: the content of curculigoside is detected by content detection item under 2020 edition of Chinese pharmacopoeia.
1. The invention carries out the influence of a physical inducer on curculigoside at the beginning of research
After domesticated seedlings of the tissue culture seedlings of the curculigo orchioides are transplanted, drought stress, salt and alkali stress, temperature difference stimulation, strong light stimulation and long-time sunlight stimulation are carried out in a growth vigorous period of 8 months, the curculigo orchioides are dug after 60 days of stress to measure the curculigoside content, each treatment quantity is 100 curculigo orchioides, the curculigo orchioides are planted by using flowerpots, and three times of each treatment are set.
(1) Comparison: stress treatment is not carried out, and conventional management is carried out under the photovoltaic panel.
(2) Drought stress: under the photovoltaic panel, the soil humidity is controlled to be about 45%.
(3) Saline-alkali stress: under a photovoltaic panel, a sodium chloride/sodium carbonate regulator (6 g/L:12 g/L) is poured once every 5 days, and the mu pouring amount is 200kg.
(4) Temperature difference stimulation: normally managing under a photovoltaic panel in the daytime, and moving the seedlings to a 10 ℃ constant-temperature incubator for dark culture at night.
(5) Strong light stimulation: the flowerpot is placed in an open place between the photovoltaic panels, and the sun is completely and directly irradiated.
(6) Long-day stimulation: and (4) placing the photovoltaic panel under the daytime for normal management, starting artificial light supplement at seven points at night, and supplementing light for four hours every day.
(7) Short-day stimulation: and moving the seedlings to a full dark condition at five pm, and moving the seedlings to a photovoltaic panel for normal management at nine pm.
Through curculigoside content determination and data statistical analysis, drought stress, saline-alkali stress and temperature difference stimulation can remarkably promote accumulation of curculigoside, wherein the dry stress and the saline-alkali stress are improved by about 1.5 times compared with a contrast, the accumulation of curculigoside cannot be promoted by prolonging or shortening the sunlight duration, and strong light stimulation cannot promote the accumulation of curculigoside but has strong inhibiting effect.
Meanwhile, drought stress, salt and alkali stress and strong light stimulation are found in the planting process, so that plants are thin and weak, rust disease is easy to occur, and the yield of medicinal materials is obviously reduced after the plants are dug.
2. Plant growth regulators were performed for single factor experiments.
After domesticated seedlings of the tissue culture seedlings of the curculigo orchioides are transplanted, NAA (naphthylacetic acid), 2,4-D, GA3 (gibberellin), PPP333 (paclobutrazol), CCC (chlormequat chloride), BR (brassinolide), KT, SA (salicylic acid), meJA (methyl jasmonate), ABA (abscisic acid) and 6-BA root irrigation tests with different concentrations are carried out in a growth vigorous period of 8 months, and the root irrigation is carried out once every 5 days, wherein the root irrigation amount per mu is 200kg.
Planting the plants under the photovoltaic panel by flowerpots every 100 plants, setting three times for each treatment, and using water spraying as a contrast.
After 60 days of treatment, digging underground rhizomes of the curculigo orchioides, and carrying out curculigo orchioides content measurement and data analysis, wherein the results show that NAA, BR, SA, ABA and MeJA have a certain promotion effect on accumulation of the curculigo orchioides, the ABA promotion effect with the concentration of 10-20 mg/L is optimal, the maximum increase is 1.2 times of that of a control, and SA, NAA, BR and MeJA with proper concentrations also have promotion effects but smaller increases, wherein the MeJA concentration is extremely difficult to control, the curculigo orchioides are extremely easy to die at a higher concentration, and no obvious effect is produced at a lower concentration.
However, whether ABA or SA is used, the leaf aging, the color graying, the green and the lusterless (similar to water shortage) are promoted to a great extent, and finally the substance accumulation of underground rhizomes is far lower than that of a control.
3. Performing a multi-factor, multi-level orthogonal test of plant growth regulators:
after domesticated seedlings of the tissue culture seedlings of the curculigo orchioides are transplanted, in a growth vigorous period of 8 months, NAA, BR, ABA and SA are used for carrying out combined tests with different concentrations, and the test design is shown in a table 2:
irrigating once every 5 days, wherein the root irrigation amount is 200 kg/mu every time, three times of treatment are set for each treatment, after 60 days of treatment, underground roots and stems of the curculigo orchioides are dug out, and the content determination and data analysis of the curculigo orchioides are carried out, and the results are shown in table 3:
as can be seen from Table 3, the effects of No. 2 and No. 3 are the best, but the effects of No. 2 and No. 3 affect the growth of Curculigo orchioides to different degrees, but the effects are obviously weaker than those of ABA and SA when single-factor experiments are carried out. It is demonstrated that both NAA and BR have antagonistic action on ABA in the growth potential of Curculigo orchioides.
As can be seen from Table 3, there is almost no difference between No. 5, no. 7, no. 9 and No. 1, and the four plant growth regulators in the single-factor test can promote the accumulation of Curculigoside to some extent, which indicates that NAA, BR and ABA have obvious antagonistic action on the accumulation of Curculigoside, with NAA being the most obvious.
4. Influence of heavy metal and amino acid on accumulation of curculigoside
After domesticated seedlings of the tissue culture seedlings of the curculigo orchioides are transplanted, root irrigation tests of four heavy metals of copper sulfate, ferrous sulfate, zinc sulfate and manganese sulfate and three amino acid regulators of tyrosine, alanine and phenylalanine with different concentrations are carried out in a vigorous growth period of 8 months, the roots are irrigated once every 5 days, and the root irrigation amount per mu is 200kg.
Planting the plants under the photovoltaic panel by using flowerpots every 100 plants, setting three times of treatment at the same time, and irrigating roots with the same amount of water as a contrast.
After the treatment for 60 days, digging out underground rhizomes of the curculigo orchioides, and carrying out curculigo orchioides content determination and data analysis, wherein the results show that the copper sulfate, the zinc sulfate and the alanine with proper concentrations can promote the accumulation of the curculigo orchioides, the tyrosine and the phenylalanine can not promote the accumulation of the curculigo orchioides, and the high-concentration ferrous sulfate and the manganese sulfate can not promote the accumulation of the curculigo orchioides but have obvious inhibition effect.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the foregoing description only for the purpose of illustrating the principles of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the invention as defined by the appended claims, specification, and equivalents thereof.
Claims (2)
1. A regulation method for promoting effective accumulation of curculigoside is characterized by comprising the following steps:
(1) Transplanting the rhizoma curculiginis seedlings, irrigating roots of the rhizoma curculiginis in the initial growth stage 6 months in the next year by using a regulator A, and irrigating roots of the rhizoma curculiginis by using a regulator B after 7-10 days;
(2) Irrigating roots of the curculigo orchioides in the initial full growth period of 8 months by using a regulator C, and irrigating roots of the curculigo orchioides by using a regulator D after 7-10 days;
(3) Irrigating the roots of the curculigo orchioides in the last growth stage of 10 months by using the regulator E, and irrigating the roots once again by using the regulator E after 7-10 days;
(4) Repeating the steps (1) to (3) every year until the coal is mined;
the regulator A consists of 5-10 mg/L abscisic acid, 2.0-3.0 g/L alanine and 3-5 mg/L copper sulfate;
the regulator B consists of 80-120 mg/L of naphthylacetic acid, 40-60 mg/L of salicylic acid and 0.1-0.2 mg/L of brassinolide;
the regulator C consists of 15-20 mg/L of abscisic acid, 2.0-3.0 g/L of alanine, 3-5 mg/L of copper sulfate and 10-15 mg/L of zinc sulfate;
the regulator D consists of 200-250 mg/L of naphthylacetic acid, 80-100 mg/L of salicylic acid and 0.2-0.3 mg/L of brassinolide;
the regulator E consists of 200-250 mg/L naphthylacetic acid, 100-120 mg/L salicylic acid, 2.0-3.0 g/L alanine, 1-2 mg/L copper sulfate and 25-30 mg/L zinc sulfate.
2. The method according to claim 1, wherein the amount of the regulators A, B, C, D, E in steps (1) to (3) is 200 to 250kg per acre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211716441.2A CN115669489B (en) | 2022-12-30 | 2022-12-30 | Regulator for promoting effective accumulation of curculigoside and regulating method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211716441.2A CN115669489B (en) | 2022-12-30 | 2022-12-30 | Regulator for promoting effective accumulation of curculigoside and regulating method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115669489A CN115669489A (en) | 2023-02-03 |
| CN115669489B true CN115669489B (en) | 2023-04-07 |
Family
ID=85057079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211716441.2A Active CN115669489B (en) | 2022-12-30 | 2022-12-30 | Regulator for promoting effective accumulation of curculigoside and regulating method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115669489B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117567201B (en) * | 2024-01-17 | 2024-03-15 | 云南省农业科学院药用植物研究所 | Method for improving domestication survival rate and growth rate of seedling of Xian Mao Zupei |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0731310A (en) * | 1993-07-20 | 1995-02-03 | Asahi Denka Kogyo Kk | Production method of seedling of curculigo plant |
| CN102123581A (en) * | 2008-06-16 | 2011-07-13 | 埃德温·亨利克斯·安东尼厄斯·霍尔曼 | Method for growing plants |
| CN102762103A (en) * | 2009-12-28 | 2012-10-31 | 加州大学评议会 | Use of a natural metabolite to increase crop production |
| WO2015125953A1 (en) * | 2014-02-24 | 2015-08-27 | 焼津水産化学工業株式会社 | Plant growth regulator, and plant growth regulation method |
| CN106305105A (en) * | 2016-08-25 | 2017-01-11 | 合肥市田然农业科技园有限公司 | Planting method of iron-rich summer spinach |
| CN108094109A (en) * | 2017-12-22 | 2018-06-01 | 广西乙木农业科技有限公司 | A kind of efficient cultivation method of Chinese cymbidium |
| CN108157074A (en) * | 2018-01-04 | 2018-06-15 | 云南农业大学 | A kind of implantation methods that thizoma curculiginis is promoted to yield positive results |
| CN109042016A (en) * | 2018-08-02 | 2018-12-21 | 紫云自治县紫香源农林科技有限责任公司 | A kind of interplanting method of bamboo and thizoma curculiginis |
| CN109258368A (en) * | 2018-11-26 | 2019-01-25 | 石家庄市农林科学研究院 | A kind of implantation methods of high yield shallot |
| CN115088723A (en) * | 2022-01-14 | 2022-09-23 | 云南省农业科学院药用植物研究所 | Plant growth regulator applied to paris polyphylla and using method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9591847B2 (en) * | 2012-10-29 | 2017-03-14 | Washington State University | Control of ripening and senescence in pre-harvest and post-harvest plants and plant materials by manipulating alternative oxidase activity |
| US20220064666A1 (en) * | 2020-08-28 | 2022-03-03 | California Institute Of Technology | Synthetic mammalian signaling circuits for robust cell population control |
-
2022
- 2022-12-30 CN CN202211716441.2A patent/CN115669489B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0731310A (en) * | 1993-07-20 | 1995-02-03 | Asahi Denka Kogyo Kk | Production method of seedling of curculigo plant |
| CN102123581A (en) * | 2008-06-16 | 2011-07-13 | 埃德温·亨利克斯·安东尼厄斯·霍尔曼 | Method for growing plants |
| CN102762103A (en) * | 2009-12-28 | 2012-10-31 | 加州大学评议会 | Use of a natural metabolite to increase crop production |
| WO2015125953A1 (en) * | 2014-02-24 | 2015-08-27 | 焼津水産化学工業株式会社 | Plant growth regulator, and plant growth regulation method |
| CN106305105A (en) * | 2016-08-25 | 2017-01-11 | 合肥市田然农业科技园有限公司 | Planting method of iron-rich summer spinach |
| CN108094109A (en) * | 2017-12-22 | 2018-06-01 | 广西乙木农业科技有限公司 | A kind of efficient cultivation method of Chinese cymbidium |
| CN108157074A (en) * | 2018-01-04 | 2018-06-15 | 云南农业大学 | A kind of implantation methods that thizoma curculiginis is promoted to yield positive results |
| CN109042016A (en) * | 2018-08-02 | 2018-12-21 | 紫云自治县紫香源农林科技有限责任公司 | A kind of interplanting method of bamboo and thizoma curculiginis |
| CN109258368A (en) * | 2018-11-26 | 2019-01-25 | 石家庄市农林科学研究院 | A kind of implantation methods of high yield shallot |
| CN115088723A (en) * | 2022-01-14 | 2022-09-23 | 云南省农业科学院药用植物研究所 | Plant growth regulator applied to paris polyphylla and using method thereof |
Non-Patent Citations (7)
| Title |
|---|
| 何冬梅等.中药微生态与中药道地性.《中国中药杂志》.2020,第45卷(第2期),第290-302页. * |
| 姚宗凡等.《常用中药种植技术》.金盾出版社,1989,(第2版),第14页. * |
| 朱伯华等.《现代种植业实用技术》.湖北科学技术出版社,2016,(第1版),第329页. * |
| 朱迪娜 ; 王磊 ; 王思彤 ; 张文生 ; .植物雌激素的研究进展.中草药.2018,(第07期),第228-233页. * |
| 郝庆秀等.不同产地三七栽培加工技术调查.《中国现代中药》.2014,第16卷(第2期),第123-129页. * |
| 鞠成国 ; 李媛媛 ; 王巍 ; 贾天柱 ; 艾雪 ; .仙茅不同炮制品对腺嘌呤致肾阳虚大鼠的作用机制分析.中国实验方剂学杂志.2014,(第16期),第34-41页. * |
| 鲜靖苹 ; 王勇 ; 马晖玲 ; 全永彪 ; .外源NO对镉胁迫下草地早熟禾幼苗根系生长及生理的影响.草原与草坪.2010,(第02期),第190-194页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115669489A (en) | 2023-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bouman et al. | Rice: feeding the billions | |
| Alam et al. | Status of homestead biodiversity in the offshore island of Bangladesh | |
| Weibin et al. | The production and research of fig (Ficus carica L.) in China | |
| CN104961545B (en) | A kind of raising salt-soda soil sugar grass emerges the preparation of quality | |
| CN115669489B (en) | Regulator for promoting effective accumulation of curculigoside and regulating method | |
| CN105367259A (en) | Novel digested-effluent organic liquid fertilizer and preparation method therefor | |
| CN103843636B (en) | A kind of organic high-calcium nutrient apple Two-way tune method | |
| CN103819250B (en) | The cultivating method of zinc-rich nutrient agent, its making method and rich zinc garlic bolt | |
| CN105052643A (en) | Organic rice cultivation method | |
| CN102763540B (en) | High-yield planting method for lavandula latifolia in sand land | |
| CN110036840A (en) | A kind of implantation methods of selenium-rich purple perilla | |
| CN104272964A (en) | Breeding method of andrographis paniculata | |
| Zaller | Competitive ability of Rumex obtusifolius against native grassland species: above-and belowground allocation of biomass and nutrients | |
| CN115583856B (en) | Compound growth regulating nutrient solution for improving quality of Ainsliaea fragrans champ and application thereof | |
| Ncise et al. | Interactive effects of light intensity and ph on growth parameters of a bulbous species (Tulbaghia violacea l.) in hydroponic cultivation and its antifungal activities | |
| CN102763547B (en) | Method for planting Lavandula latifolia on sand soil land parcels | |
| Reja et al. | Effect of gibberellic acid (GA3) on morpho-physiological traits and yield performance of chickpea (Cicer arietinum L.) | |
| CN105210620A (en) | A kind of mating system of Herba Andrographitis | |
| CN105130593A (en) | Sodium alginate oligosaccharide complex and applications of sodium alginate oligosaccharide complex in release of copper toxicity on crops | |
| CN109769446A (en) | Management method asparagus seed farm fertilising and irrigated | |
| Sitpayeva et al. | Bringing medicinal plants of the native flora of the northern Tien Shan into cultivation | |
| Khuankaew et al. | Effects of gibberellin application on growth and development of Curcuma alismatifolia Gagnep | |
| Lu et al. | Leymus chinensis tolerates mowing disturbance by maintaining photosynthesis in saline-alkali heterogeneous habitats | |
| CN109845601A (en) | Selenium-rich zinc shrimp rice | |
| CN107211709A (en) | A kind of breeding method of Snakegourd Fruit seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20230203 Assignee: Nujiang Angda Biotechnology Development Co.,Ltd. Assignor: INSTITUTE OF MEDICINAL PLANTS, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980054565 Denomination of invention: A regulator and regulatory method for promoting effective accumulation of Xianmao glycoside Granted publication date: 20230407 License type: Common License Record date: 20231228 |