CN115607499A - Ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening and application thereof - Google Patents

Abstract

The application relates to the field of cosmetics, and particularly discloses a ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening, which comprises the following components in parts by mass: 0.001-0.005 part of ginseng extract; 0.001-0.004 parts of ganoderma lucidum extract; 0.005-0.02 parts of asparagus extract; 0.001-0.004 parts of cubilose extract; acetyl hexapeptide-8.01-0.04 parts; dipeptide-2.0005-0.0010 part; oligopeptide-1.0002-0.0005 part; 0.0003-0.0005 part of palmitoyl tetrapeptide-7; 0.007-0.009 parts of preservative; 1.192-1.5953 parts of a humectant; 1.721-2.182 parts of solvent. This application has and makes cosmetics can reach multiple anti-wrinkle effect of tightening through multiple way.

Description

Ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening and application thereof

Technical Field

The application relates to the field of cosmetics, in particular to a ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and firming skin and application thereof.

Background

Wrinkles are one of the signs of skin aging, and are caused by the fact that the human body is affected by endogenous or exogenous factors, so that the supporting tissues and the structure of the skin are changed, the water retention capacity of the skin is lost, the arrangement sequence of elastic fibers and collagen is changed, the mechanical force and elasticity of the skin are reduced, depressions are formed, and finally wrinkles are formed. Many factors causing wrinkles, such as active oxygen, ultraviolet irradiation, free radicals, inflammation and the like, and damage caused by the factors gradually accumulates with the age, and finally wrinkles, looseness and aging of the skin are caused.

However, some cosmetics for resisting skin aging have single anti-aging action way and poor anti-aging effect in the market. Thus, there is still room for improvement.

Disclosure of Invention

In order to enable cosmetics to achieve multiple anti-wrinkle and firming effects through multiple ways, and meanwhile, the cosmetics also have excellent whitening and moisturizing effects and enable skin to be glossy and moist, the application provides the ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and firming and the application thereof.

In a first aspect, the application provides a ginseng ganoderma lucidum polypeptide composition for resisting wrinkles and tightening, which adopts the following technical scheme:

the ginseng and lucid ganoderma polypeptide composition for resisting wrinkles and tightening comprises the following components in parts by mass:

0.001-0.005 part of ginseng extract;

0.001-0.004 parts of Chinese ganoderma lucidum extract;

0.005-0.02 parts of asparagus extract;

0.001-0.004 parts of cubilose extract;

acetyl hexapeptide-8.01-0.04 parts;

dipeptide-2.0005-0.0010 weight portions;

oligopeptide-1.0002-0.0005 part;

0.0003-0.0005 part of palmitoyl tetrapeptide-7;

0.007-0.009 parts of preservative;

1.192-1.5953 parts of a humectant;

1.721-2.182 parts of solvent.

By adopting the technical scheme, the ginseng extract contains active ingredients such as ginsenoside, essential oil, ginseng acid, vitamins and the like, so that the ginseng extract can inhibit melanocytes from generating melanin, reduce melanin granules, and has the effects of delaying skin aging, increasing skin elasticity and reducing skin wrinkles.

Because the ganoderma contains active ingredients such as ganoderma lucidum polysaccharide, triterpenes, amino acid and the like, the ganoderma lucidum can regulate and control the expression of skin cell senescence genes, repair DNA, reduce the oxidative stress damage of keratinocytes, promote cell proliferation, increase the thickness of epidermis and dermis, improve the oxidation resistance of skin and have the effects of repairing and resisting aging.

The radix asparagi extract contains multiple glycosides such as glycoside, steroid glycoside, saponin, etc., and has effects of resisting oxidation, delaying aging, keeping moisture and moistening; the nidus Collocaliae extract can promote macrophage to release interleukin-1 to accelerate III type collagen synthesis, and has anti-wrinkle effect.

By adding acetyl hexapeptide-8, the acetyl hexapeptide-8 is a bioactive polypeptide, can inhibit the release of the nerve conduction element acetylcholine to reduce the contraction of muscles, can reduce the existing facial wrinkles and can effectively prevent the generation of new wrinkles. The dipeptide-2 can inhibit the conversion of angiotensin I into angiotensin II, thereby improving blood circulation, and the oligopeptide-1 is formed by combining wheat and soybean protein, can stimulate the synthesis of collagen, and has the effect of tightening skin. Palmitoyl tetrapeptide-7 can be used for tightening skin by stimulating laminin IV and collagen VII, reducing interleukin production, relieving inflammation, and preventing wrinkle and flabbiness.

Therefore, the ginseng extract, the Chinese ganoderma lucidum extract, the asparagus extract, the cubilose extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7 are mutually promoted and supported by a specific proportion, and the components and the proportion are cooperatively matched, so that multiple anti-wrinkle and firming effects are achieved through multiple ways, the ginseng ganoderma lucidum polypeptide composition has a good anti-wrinkle effect, and also has excellent whitening and moisturizing effects, and the skin is glossy and moist.

Preferably, the mass ratio of the ginseng extract, the ganoderma lucidum extract, the asparagus extract, the cubilose extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7 is 0.003:0.002:0.01:0.002:0.003:0.0009:0.00045:0.00045.

by adopting the technical scheme, the skin relaxation is further favorably reduced, the firmness is improved, and the wrinkles are removed, so that the anti-wrinkle and firmness effects of the ginseng-ganoderma lucidum polypeptide composition are further improved.

Preferably, the preservative is phenoxyethanol.

By adopting the technical scheme, active substances in the ginseng extract, the Chinese ganoderma extract, the asparagus extract and the cubilose extract are more stable and are not easy to lose activity, and the ginseng extract, the Chinese ganoderma extract, the asparagus extract and the cubilose extract are better matched with acetyl hexapeptide-8, dipeptide-2, oligopeptide-1 and palmitoyl tetrapeptide-7 in a synergistic manner, so that the anti-wrinkle and firming effects of the ginseng and ganoderma polypeptide composition are not easy to influence.

Preferably, the humectant is one or more of ethylhexyl glycerin, butanediol, glycerin, 1, 2-hexanediol, and sodium hyaluronate.

Preferably, the humectant is a compound of ethylhexyl glycerin, butanediol, glycerin, 1, 2-hexanediol and sodium hyaluronate.

Preferably, the mass ratio of the ethylhexyl glycerol to the butanediol to the glycerol to the 1, 2-hexanediol to the sodium hyaluronate is 0.0002:0.02:1.5:0.03:0.003.

by adopting the technical scheme, the ginseng ganoderma lucidum polypeptide composition has a good moisturizing effect, and further has multiple effects.

In a second aspect, the application provides an application of a ginseng ganoderma lucidum polypeptide composition for resisting wrinkles and tightening skin, which adopts the following technical scheme:

the application of the ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening bodies is characterized in that the ginseng and ganoderma lucidum polypeptide composition is added into cream, and the cream containing the ginseng and ganoderma lucidum polypeptide composition comprises the following components in percentage by mass:

phase A: 6-9% of caprylic/capric triglyceride; 6-8% of isononyl isononanoate; 5-7% of C12-15 alcohol benzoate; 4-6% of shea butter extract; 2-5% of polydimethylsiloxane; 1-3% of C20-24 alkyl polydimethylsiloxane; 1.5-2% of PEG-20 methyl glucose sesquistearate; 0.6 to 0.8 percent of PEG-100 stearate; 0.7-0.9% of glycerol stearate; cetostearyl alcohol 1.3-1.6%; 1-1.3% of methyl glucose sesquistearate; 0.2 to 0.4 percent of potassium lauryl phosphate; 0.6 to 0.8 percent of water; mineral oil 0.8-1.2%; 0.1 to 0.4 percent of methyl hydroxybenzoate;

phase B: 13-16% of dipropylene glycol; 8-10% of glycerol; acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.3-0.5%; 0.1-0.4% of acryloyl dimethyl ammonium taurate/VP copolymer; 0.01 to 0.03 percent of sodium hyaluronate; 1 to 1.3 percent of water; 0.6 to 0.9 percent of chitosan glycolate;

and C phase: 0.3 to 0.5 percent of triethanolamine;

phase D: 0.2 to 0.3 percent of water; 0.2 to 0.4 percent of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.1 to 0.2 percent of isohexadecane; polysorbate-80.05-0.08%;

phase E: 0.5 to 0.7 percent of water; 0.3-0.5% of glycerol; 0.004-0.006% of glycerol polyacrylate; 0.0004 to 0.0006 percent of methyl hydroxybenzoate; 0.0001-0.0003% of propyl hydroxybenzoate; 2-4% of ginseng and ganoderma lucidum polypeptide composition; 0.02-0.04% of caprylyl hydroximic acid; 0.03 to 0.05 percent of 1, 3-propylene glycol; 0.1 to 0.2 percent of butanediol; glycosphingolipids 0.03-0.06%; 0.4 to 0.5 percent of phenoxyethanol; 0.03-0.05% of ethylhexyl glycerol; 0.3 to 0.5 percent of essence;

the balance of water; the total mass percentage is 100 percent.

By adopting the technical scheme, the ginseng and ganoderma lucidum polypeptide composition is added into the cream, so that the cream has better effects of resisting wrinkles and tightening, has better whitening and moisturizing effects, and meets the requirements of people on multiple functions of cosmetics.

In a third aspect, the application provides an application of the ginseng ganoderma lucidum polypeptide composition for resisting wrinkles and tightening, and the following technical scheme is adopted:

the application of the ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening bodies is characterized in that the ginseng and ganoderma lucidum polypeptide composition is added into milk, and the milk containing the ginseng and ganoderma lucidum polypeptide composition comprises the following components in percentage by mass:

phase A: 2-4% of dioctyl carbonate; 1.5-2% of cyclopentasiloxane; 1-1.3% of cyclohexasiloxane; caprylic/capric triglyceride 1-3%; c14-22 alcohol 0.6-0.9%; 0.1-0.3% of C12-20 alkyl glucoside; 0.5-0.7% of polydimethylsiloxane; 0.1 to 0.3 percent of PEG-100 stearate; 0.2-0.4% of glycerol stearate; cetostearyl alcohol 0.2-0.5%; 0.1 to 0.4 percent of methyl hydroxybenzoate;

phase B: 2-4% of butanediol; 1-1.5% of propylene glycol; 0.1 to 0.3 percent of acryloyl dimethyl ammonium taurate/VP copolymer; 0.05 to 0.1 percent of allantoin; 0.06-0.09% of xanthan gum; 0.03-0.06% of EDTA disodium; 0.02-0.04% of sodium hyaluronate;

and C phase: 0.08 to 0.1 percent of water; 0.1-0.2% of sodium acrylate/acryloyl dimethyl sodium taurate copolymer; 0.05 to 0.07 percent of isohexadecane; polysorbate-80.02-0.03%;

phase D: 1-1.5% of propylene glycol; 1-2% of water; 1-1.5% of chitosan glycolate; polyglycerol-10.5-1%; raspberry ketone glucoside 0.3-0.6%; carnosine 0.4-0.7%; 0.3 to 0.5 percent of p-hydroxyacetophenone; 0.5 to 0.8 percent of 1, 2-hexanediol; 3-5% of ginseng and ganoderma lucidum polypeptide composition; 0.04 to 0.05 percent of phenoxyethanol; 0.004-0.006% of ethylhexyl glycerol; 0.1 to 0.2 percent of essence;

e phase: CI75470 0.0001-0.0002%; CI 19140.0001-0.0002%; CI 75100.0003-0.0005%;

the balance of water, the total mass percent is 100%.

By adopting the technical scheme, the ginseng and ganoderma lucidum polypeptide composition is added into milk, so that the milk has a good anti-wrinkle and firming effect, and also has good whitening and moisturizing effects.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the ginseng and ganoderma lucidum polypeptide composition has the advantages that ginseng extract, ganoderma lucidum extract, asparagus extract, cubilose extract, acetyl hexapeptide-8, dipeptide-2, oligopeptide-1 and palmitoyl tetrapeptide-7 in specific proportions are mutually promoted and supported, the components are matched with the proportions in a synergistic manner, multiple anti-wrinkle and tightening effects are achieved through multiple ways, the ginseng and ganoderma lucidum polypeptide composition has a good anti-wrinkle effect, excellent whitening and moisturizing effects are achieved, and skin is enabled to be glossy and moist.

2. By adopting phenoxyethanol with a specific proportion as a preservative, active substances in the ginseng extract, the ganoderma sinense extract, the asparagus extract and the cubilose extract are more stable and are not easy to lose activity, and are better cooperated with acetyl hexapeptide-8, dipeptide-2, oligopeptide-1 and palmitoyl tetrapeptide-7, so that the anti-wrinkle and firming effects of the ginseng-ganoderma lucidum polypeptide composition are not easy to be influenced.

3. By adding the ginseng and ganoderma lucidum polypeptide composition into cream or milk, the cream or milk has better effects of resisting wrinkles and tightening, and also has better whitening and moisturizing effects, so that the requirements of people on multiple functions of cosmetics are met.

Detailed Description

The present application will be described in further detail with reference to examples.

Examples 1 to 3

A ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening bodies comprises the following components:

a ginseng extract; ganoderma extract of China; an asparagus root extract; nidus Collocaliae extract; acetyl hexapeptide-8; a dipeptide-2; oligopeptide-1; palmitoyl tetrapeptide-7; a preservative; a humectant; a solvent;

wherein the antiseptic is phenoxyethanol; the humectant is selected from ethylhexyl glycerin, butanediol, glycerin, 1, 2-hexanediol, and sodium hyaluronate; the solvent is water.

A preparation method of ginseng and ganoderma lucidum polypeptide composition for resisting wrinkles and tightening comprises the steps of respectively adding ginseng extract, chinese ganoderma lucidum extract, asparagus extract, cubilose extract, acetyl hexapeptide-8, dipeptide-2, oligopeptide-1, palmitoyl tetrapeptide-7, preservative, humectant and solvent into a stirring kettle, and uniformly stirring at the rotating speed of 50r/min to obtain the ginseng and ganoderma lucidum polypeptide composition.

Comparative examples 1 to 2

The difference from example 3 is that: the dosage of the ginseng extract, the ganoderma lucidum extract, the asparagus extract, the cubilose extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7 are not within the protection range of the application.

The components and the amounts used in examples 1 to 3 and comparative examples 1 to 2 are shown in Table 1, and the units of the amounts used in Table 1 are kg.

TABLE 1

Comparative example 3

The difference from example 3 is that: equal amount of water was used instead of the ginseng extract and acetyl hexapeptide-8.

Comparative example 4

The difference from example 3 is that: equal amount of water is used to replace the ganoderma lucidum extract and oligopeptide-1.

Comparative example 5

The difference from example 3 is that: equal amounts of water were substituted for the Asparagus extract and palmitoyl tetrapeptide-7.

Comparative example 6

The difference from example 3 is that: equal amount of water was used to replace cubilose extract and dipeptide-2.

Experiment 1 inhibition ratio of Elastase

The inhibition rate of elastase was determined by spectrophotometry. Congo red-elastin is used as a substrate, after elastin is decomposed by enzyme, congo red falls off, and the content of Congo red in the solution is measured to characterize the enzyme activity. Respectively dissolving the ginseng and ganoderma lucidum polypeptide compositions prepared in examples 1-3 and comparative examples 1-6 in water to prepare test solutions with the concentration of 6 mg/mL; congo red-elastin and boric acid buffer solution were added to the Erlenmeyer flask, preheated in a 37 ℃ water bath for 10min, and then the above test solution and 0.2mg/mL elastase solution were added, as shown in Table 2.

TABLE 2

The reaction system is placed in a constant temperature water bath oscillator at 37 ℃ and shaken for 20min, 5.0ml of phosphate buffer solution with pH6.0 is immediately added for mixing and stopping the reaction, and the mixture is centrifuged for 15min at 5000 r/min. Accurately sucking 2.0mL of supernatant, adding 2.0mL of mixed solution of pH8.8 boric acid buffer solution and pH6.0 phosphate buffer solution, fully shaking and measuring the absorbance at 495nm, wherein the inhibition rate of the elastase is calculated as follows: the inhibition ratio of elastase = (1- (A1-A2)/(B1-B2)) × 100%.

The higher the inhibition rate of elastase, the better the anti-wrinkle effect of the ginseng ganoderma lucidum polypeptide composition.

EXPERIMENT 2 DPPH-FREE RADICAL CLEANING

In the experiment, referring to a cosmetic-free radical (DPPH) removal experimental method (T/SHRH 006-2018), the DPPH free radical removal rates (%) of the ginseng ganoderma lucidum polypeptide compositions prepared in the above examples 1-3 and comparative examples 1-6 are respectively detected, and the higher the DPPH free radical removal rate is, the better the anti-wrinkle effect of the ginseng ganoderma lucidum polypeptide composition is.

Experiment 3 promotion rate of collagen type III Synthesis

The ginseng-ganoderma lucidum polypeptide compositions prepared in the above examples 1-3 and comparative examples 1-6 were used as samples to be tested.

The detection method comprises the following steps: human fibroblasts cultured in 10-vol FBS-containing DMEM medium were seeded in a 24-well plate, and after cell attachment, medium exchange was performed using 0.25-vol FBS-containing DMEM medium and 250. Mu.M magnesium ascorbyl phosphate-containing DMEM medium, and the aforementioned test samples were added, respectively. After three days of culture, the culture medium supernatant was recovered, centrifuged, and the type III collagen in the resulting supernatant was measured.

Among them, the evaluation method of the collagen type III biosynthesis ability in the cell is to measure the content of the terminal peptide (Procollagen type III-peptide: PIII P for short) of the collagen type III secreted in the supernatant by using "RIA-gnostP III P (PIII P) measurement kit". And evaluating the strength of the anti-wrinkle effect of the sample through the synthesis promotion rate of the type III collagen. The calculation formula is as follows:

promotion rate of collagen type iii synthesis = (a-B)/B × 100%;

a: the amount of type III collagen produced after addition of the test sample;

b: amount of type iii collagen produced without addition of test sample.

The results of the above experiments are shown in Table 3.

TABLE 3

The results of comparison with example 3 according to comparative examples 3 to 6 in Table 3 were obtained, wherein no ginseng extract and acetyl hexapeptide-8 were added in comparative example 3, no Ganoderma sinense extract and oligopeptide-1 were added in comparative example 4, no asparagus extract and palmitoyl tetrapeptide-7 were added in comparative example 5, no cubilose extract and dipeptide-2 were added in comparative example 6, and the elastase inhibitory rate, DPPH radical scavenging rate, collagen type III synthesis promoting rate of the ginseng ganoderma polypeptide compositions of comparative examples 3 to 6 were lower than those of example 3. However, in example 3, the ginseng extract, the ganoderma sinense extract, the asparagus extract, the bird's nest extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7 are added at the same time, and the elastase inhibition rate, the DPPH free radical clearance rate and the collagen type III synthesis promotion rate of the ginseng ganoderma lucidum polypeptide composition are all higher than those of comparative examples 3-6, which shows that only by adopting the mutual synergistic cooperation of the ginseng extract, the ganoderma sinense extract, the asparagus extract, the bird's nest extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7, the ginseng ganoderma lucidum polypeptide composition can achieve multiple anti-wrinkle and firming effects through multiple ways.

According to the comparison of comparative examples 1-2 in table 3 with example 3, the amounts of ginseng extract, ganoderma sinense extract, asparagus extract, bird's nest extract, acetyl hexapeptide-8, dipeptide-2, oligopeptide-1 and palmitoyl tetrapeptide-7 in comparative examples 1-2 are not within the protection range of the present application, the inhibition rate of elastase, the clearance rate of DPPH free radicals and the synthesis promotion rate of type iii collagen of the ginseng ganoderma lucidum polypeptide composition are all lower than those in example 3, which shows that the effects of wrinkle resistance and firming can be achieved through various ways only when the amounts of ginseng extract, ganoderma sinense extract, asparagus extract, bird's nest extract, acetyl hexapeptide-8, dipeptide-2, oligopeptide-1 and palmitoyl tetrapeptide-7 are within the protection range of the present application, and the ginseng ganoderma lucidum polypeptide composition has better wrinkle resistance effect. The above effects cannot be achieved by any ratio.

Application example 1

A cream containing ginseng and ganoderma lucidum polypeptide composition comprises the following components in parts by mass:

phase A: 6kg of caprylic/capric triglyceride; 6kg of isononyl isononanoate; 5kg of C12-15 alcohol benzoate; 4kg of shea butter extract; 2kg of polydimethylsiloxane; 1kg of C20-24 alkyl polydimethylsiloxane; 1.5kg of PEG-20 methyl glucose sesquistearate; 0.6kg of PEG-100 stearate; 0.7kg of glycerol stearate; cetearyl alcohol 1.3kg; 1kg of methyl glucose sesquistearate; 0.2kg of potassium lauryl phosphate; 0.6kg of water; 0.8kg of mineral oil; 0.1kg of methyl hydroxybenzoate;

phase B: 42.626kg of water; 13kg of dipropylene glycol; 8kg of glycerol; 0.3kg of acrylic acid (ester)/C10-30 alkanol acrylate crosslinked polymer; 0.1kg of acryloyl dimethyl ammonium taurate/VP copolymer; 0.01kg of sodium hyaluronate; 0.6kg of chitosan glycolate;

and C phase: 0.3kg of triethanolamine;

phase D: 0.2kg of water; 0.2kg of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.1kg of isohexadecane; polysorbate-80.05kg;

phase E: 0.5kg of water; 0.3kg of glycerol; 0.004kg of glycerol polyacrylate; 0.0004kg of methylparaben; 0.0001kg propyl hydroxybenzoate; 2kg of ginseng and ganoderma lucidum polypeptide composition; 0.02kg of caprylyl hydroximic acid; 0.03kg of 1, 3-propylene glycol; 0.1kg of butanediol; glycosphingolipids 0.03kg; 0.4kg of phenoxyethanol; 0.03kg of ethylhexyl glycerol; 0.3kg of essence;

wherein, the ginseng ganoderma lucidum polypeptide composition prepared in the embodiment 3 is adopted.

A method for preparing cream containing ginseng and ganoderma lucidum polypeptide composition comprises the following steps:

s1: respectively putting the components in the phase A into an oil phase pot, heating to 80 ℃, uniformly stirring at the rotating speed of 30r/min, and preserving heat for 20min;

s2: putting the components in the phase B into a water phase pot respectively, heating to 85 ℃, stirring and dissolving at the rotating speed of 35r/min, and keeping the temperature for 30min;

s3: then sucking the materials in the water phase pot into an emulsifying pot heated to 85 ℃ in vacuum, sucking the materials in the oil phase pot again, stirring at 35r/min for 5min, homogenizing at 3000r/min for 5min, adding the components in the C phase, homogenizing in vacuum for 1min after stirring uniformly, preserving heat for 10min, and then starting to circularly cool;

s4: cooling to 50 deg.C, adding the components of phase D, homogenizing at low speed for 1min, and stirring;

s5: continuously cooling to 45 ℃, adding the components in the phase E, and uniformly stirring at the rotating speed of 25 r/min;

s6: and (4) reducing the temperature to 38 ℃, filtering and discharging after the inspection is qualified.

Application example 2

The difference from application example 1 is that: the dosage of each component is different;

a cream containing a ginseng and ganoderma lucidum polypeptide composition comprises the following components in parts by mass:

phase A: 9kg of caprylic/capric triglyceride; 8kg of isononyl isononanoate; 7kg of C12-15 alcohol benzoate; 6kg of shea butter extract; 5kg of polydimethylsiloxane; 3kg of C20-24 alkyl polydimethylsiloxane; 2kg of PEG-20 methyl glucose sesquistearate; 0.8kg of PEG-100 stearate; 0.9kg of glycerol stearate; cetearyl alcohol 1.6kg; 1.3kg of methyl glucose sesquistearate; 0.4kg of potassium lauryl phosphate; 0.8kg of water; 1.2kg of mineral oil; 0.4kg of methylparaben;

phase B: 16.683kg of water; 16kg of dipropylene glycol; 10kg of glycerol; 0.5kg of acrylic acid (ester)/C10-30 alkanol acrylate crosslinked polymer; 0.4kg of an acryloyl dimethyl ammonium taurate/VP copolymer; 0.03kg of sodium hyaluronate; 0.9kg of chitosan glycolate;

and C phase: 0.5kg of triethanolamine;

phase D: 0.3kg of water; 0.4kg of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.2kg of isohexadecane; polysorbate-80.08kg;

phase E: 0.7kg of water; 0.5kg of glycerol; 0.006kg of glycerol polyacrylate; 0.0006kg of methylparaben; 0.0003kg of propyl hydroxybenzoate; 4kg of ginseng and ganoderma lucidum polypeptide composition; 0.04kg of caprylyl hydroximic acid; 0.05kg of 1, 3-propylene glycol; 0.2kg of butanediol; glycosphingolipids 0.06kg; 0.5kg of phenoxyethanol; 0.05kg of ethylhexyl glycerol; 0.5kg of essence.

Wherein, the ginseng ganoderma lucidum polypeptide composition prepared in the embodiment 3 is adopted.

Blank control group 1

The difference from application example 2 is that: equal amount of water is used to replace the ginseng ganoderma polypeptide composition, i.e. the ginseng ganoderma polypeptide composition is not added.

Application example 3

A milk containing ginseng and ganoderma lucidum polypeptide composition comprises the following components in parts by mass:

phase A: 2kg of dioctyl carbonate; 1.5kg of cyclopentasiloxane; 1kg of cyclohexasiloxane; 1kg of caprylic/capric triglyceride; 0.6kg of C14-22 alcohol; 0.1kg of C12-20 alkyl glucoside; 0.5kg of polydimethylsiloxane; 0.1kg of PEG-100 stearate; 0.2kg of glycerol stearate; 0.2kg of cetearyl alcohol; 0.1kg of methyl hydroxybenzoate;

phase B: 81.045kg of water; 2kg of butanediol; 1kg of propylene glycol; 0.1kg of acryloyl dimethyl ammonium taurate/VP copolymer; allantoin 0.05kg; 0.06kg of xanthan gum; 0.03kg of EDTA disodium; 0.02kg of sodium hyaluronate;

and C phase: 0.08kg of water; 0.1kg of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.05kg of isohexadecane; polysorbate-80.02kg;

phase D: 1kg of propylene glycol; 1kg of water; 1kg of chitosan glycolate; polyglycerol-10.5kg; raspberry ketone glucoside 0.3kg; carnosine 0.4kg; 0.3kg of p-hydroxyacetophenone; 0.5kg of 1, 2-hexanediol; 3kg of ginseng and ganoderma lucidum polypeptide composition; 0.04kg of phenoxyethanol; 0.004kg of ethylhexyl glycerol; 0.1kg of essence;

phase E: CI 75470.0001 kg; CI 19140.0001 kg; CI 75100.0003 kg;

wherein, the ginseng ganoderma lucidum polypeptide composition prepared in the embodiment 3 is adopted.

A method for preparing milk containing ginseng and ganoderma lucidum polypeptide composition comprises the following steps:

s1: respectively putting the components in the phase A into an oil phase pot, heating to 80 ℃, uniformly stirring at the rotating speed of 35r/min, and preserving heat for 30min;

s2: uniformly mixing the components in the phase B in a water phase pot, adding water, heating to 85 ℃ while stirring, preserving heat for 30min, and uniformly stirring and dissolving;

s3: heating an emulsifying pot to 85 ℃, pumping the materials in a water phase pot into the emulsifying pot, pumping the oil phase materials while stirring, and homogenizing at 3000r/min for 5min; then adding the components in the phase C, homogenizing for 3min, stirring at the speed of 35r/min, and cooling;

s4: cooling to 45 ℃, respectively adding the components in the phase D, and uniformly stirring at the rotating speed of 40 r/min;

s5: slowly adding the components in the phase E, and toning to be consistent with the sample plate;

s6: cooling to 38 ℃, filtering and discharging after the inspection is qualified.

Application example 4

The difference from application example 3 is that: the dosage of each component is different;

a milk containing ginseng and ganoderma lucidum polypeptide composition comprises the following components in parts by mass:

phase A: 4kg of dioctyl carbonate; 2kg of cyclopentasiloxane; 1.3kg of cyclohexasiloxane; 3kg of caprylic/capric triglyceride; 0.9kg of C14-22 alcohol; 0.3kg of C12-20 alkyl glucoside; 0.7kg of polydimethylsiloxane; 0.3kg of PEG-100 stearate; 0.4kg of glycerol stearate; 0.5kg of cetearyl alcohol; 0.4kg of methylparaben;

phase B: 65.853kg of water; 4kg of butanediol; 1.5kg of propylene glycol; 0.3kg of an acryloyl dimethyl taurate/VP copolymer; 0.1kg of allantoin; 0.09kg of xanthan gum; 0.06kg of EDTA disodium; 0.04kg of sodium hyaluronate;

and C phase: 0.1kg of water; 0.2kg of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.07kg of isohexadecane; polysorbate-80.03kg;

phase D: 1.5kg of propylene glycol; 2kg of water; 1.5kg of chitosan glycolate; 10kg of polyglycerol; 0.6kg of raspberry ketone glucoside; carnosine 0.7kg; 0.5kg of p-hydroxyacetophenone; 0.8kg of 1, 2-hexanediol; 5kg of ginseng and ganoderma lucidum polypeptide composition; 0.05kg of phenoxyethanol; 0.006kg of ethylhexyl glycerol; 0.2kg of essence;

phase E: CI75470 0.0002kg; CI 19140.0002 kg; CI 75100.0005 kg;

wherein, the ginseng ganoderma lucidum polypeptide composition prepared in the embodiment 3 is adopted.

Blank control group 2

The difference from application example 3 is that: equal amount of water is used to replace the ginseng ganoderma lucidum polypeptide composition.

Experiment 4 test subject experience test

The test environment temperature is 20-22 ℃, the humidity is 40-60%, and real-time dynamic monitoring is carried out. The subjects were 18 to 45 years old, both male and female, and 30 subjects were numbered, and each subject applied the cream containing the ginseng ganoderma polypeptide composition prepared in examples 1 to 2 and the milk containing the ginseng ganoderma polypeptide composition prepared in examples 3 to 4 to the face and neck in the same manner.

Preparation before testing: any product (cosmetics or external medicines) cannot be used for 2-3 days before the test of the tested part, and water cannot be contacted for 1-3 hours. Before the test, the testee needs to uniformly clean the face and the neck and wipe the face and the neck clean with dry facial tissues. After cleaning, the subject's face and neck are marked with measurement areas. Before formal test, the people should sit still in a room meeting the standard for 30min, cannot drink water, and are placed in a test state to keep relaxed.

The testing steps are as follows: in the experiment, the face and neck were marked with 2cm × 2cm test areas, and the face and neck were marked with multiple areas at 1cm intervals. The multiple regions are uniformly distributed on the face and neck. The test subjects were respectively smeared with the cream containing the ginseng and ganoderma lucidum polypeptide composition prepared in practical examples 1-2, the milk containing the ginseng and ganoderma lucidum polypeptide composition prepared in practical examples 3-4 and blank control groups 1-2, and the test samples were expressed as (3.0)

0.1）mg/cm ² The latex finger cot is used for homogenizing the test productSpread into the test area and each sample was applied in the morning and evening, respectively, for a period of one week.

After application, 30 volunteers were scored for skin hydration, skin elasticity, wrinkle changes, and skin lightening changes (0-100 points), respectively.

The scoring criteria for each of the above items are shown in table 4:

TABLE 4

Whether the moisturizing effect of the cream or the milk is good or not can be obtained through the grading of the skin moisture of the subject; whether the anti-wrinkle effect of the cream or the milk is good or not can be obtained through the scoring of the wrinkle change of the skin and the skin elasticity of the subject; whether the whitening effect of the cream or the milk is good or not can be obtained by scoring the change of the skin lightening of the skin by the subject. The results are shown in Table 5.

TABLE 5

According to the comparison of application examples 1-2 in the table 5 with a blank control group 1, the ginseng and ganoderma lucidum polypeptide composition is added into the cream in the application examples 1-2, the wrinkle change and skin elasticity score are higher than that of the blank control group 1, and the water wetness score and skin brightening change score are higher than that of the blank control group 1, so that the ginseng and ganoderma lucidum polypeptide composition is added into the cream, the anti-wrinkle effect of the cream can be improved, and the moisturizing and whitening effects of the cream can be improved.

According to the comparison between the application examples 3-4 in the table 5 and the blank control group 2, the ginseng and ganoderma lucidum polypeptide composition is added into the milk in the application examples 3-4, the wrinkle change and the skin elasticity are respectively higher than those in the blank control group 1, and the water wettability and the skin brightness change are respectively higher than those in the blank control group 2, so that the addition of the ginseng and ganoderma lucidum polypeptide composition into the milk can improve the anti-wrinkle effect of the milk, and is beneficial to improving the moisturizing and whitening effects of the milk.

The above are preferred embodiments of the present application, and the scope of protection of the present application is not limited thereto, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (8)

1. The ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening is characterized by comprising the following components in parts by weight: the paint comprises the following components in parts by mass:

0.001-0.005 part of ginseng extract;

0.001-0.004 parts of ganoderma lucidum extract;

0.005-0.02 part of asparagus extract;

0.001-0.004 parts of cubilose extract;

acetyl hexapeptide-8.01-0.04 parts;

dipeptide-2.0005-0.0010 part;

oligopeptide-1.0002-0.0005 part;

palmitoyl tetrapeptide-7.0003-0.0005 parts;

0.007-0.009 parts of preservative;

1.192-1.5953 parts of a humectant;

1.721-2.182 parts of solvent.

2. The ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening according to claim 1, wherein the ginseng-ganoderma lucidum polypeptide composition comprises the following components in percentage by weight: the mass ratio of the ginseng extract, the Chinese ganoderma extract, the asparagus extract, the cubilose extract, the acetyl hexapeptide-8, the dipeptide-2, the oligopeptide-1 and the palmitoyl tetrapeptide-7 is 0.003:0.002:0.01:0.002:0.003:0.0009:0.00045:0.00045.

3. the ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening according to claim 1, wherein the ginseng-ganoderma lucidum polypeptide composition comprises the following components in percentage by weight: the preservative is phenoxyethanol.

4. The ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening according to claim 1, wherein the ginseng-ganoderma lucidum polypeptide composition comprises the following components in percentage by weight: the humectant is one or more of ethylhexyl glycerin, butanediol, glycerin, 1, 2-hexanediol and sodium hyaluronate.

5. The ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening according to claim 4, wherein the composition comprises the following components in percentage by weight: the humectant is a compound of ethylhexyl glycerin, butanediol, glycerin, 1, 2-hexanediol and sodium hyaluronate.

6. The ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening according to claim 5, wherein the ginseng-ganoderma lucidum polypeptide composition comprises the following components in percentage by weight: the mass ratio of the ethylhexyl glycerin to the butanediol to the glycerin to the 1, 2-hexanediol to the sodium hyaluronate is 0.0002:0.02:1.5:0.03:0.003.

7. the application of the ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening bodies is characterized in that: adding the ginseng ganoderma lucidum polypeptide composition according to any one of claims 1-6 into cream, wherein the cream containing the ginseng ganoderma lucidum polypeptide composition comprises the following components in percentage by mass:

phase A: 6-9% of caprylic/capric triglyceride; 6-8% of isononyl isononanoate; 5-7% of C12-15 alcohol benzoate; 4-6% of shea butter extract; 2-5% of polydimethylsiloxane; 1-3% of C20-24 alkyl polydimethylsiloxane; 1.5-2% of PEG-20 methyl glucose sesquistearate; 0.6 to 0.8 percent of PEG-100 stearate; 0.7-0.9% of glycerol stearate; cetostearyl alcohol 1.3-1.6%; 1-1.3% of methyl glucose sesquistearate; 0.2 to 0.4 percent of potassium lauryl phosphate; 0.6 to 0.8 percent of water; mineral oil 0.8-1.2%; 0.1 to 0.4 percent of methyl hydroxybenzoate;

phase B: 13-16% of dipropylene glycol; 8-10% of glycerol; acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.3-0.5%; 0.1-0.4% of acryloyl dimethyl ammonium taurate/VP copolymer; 0.01 to 0.03 percent of sodium hyaluronate; 1 to 1.3 percent of water; 0.6 to 0.9 percent of chitosan glycolate;

and C phase: 0.3 to 0.5 percent of triethanolamine;

phase D: 0.2 to 0.3 percent of water; 0.2 to 0.4 percent of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.1 to 0.2 percent of isohexadecane; polysorbate-80.05-0.08%;

phase E: 0.5 to 0.7 percent of water; 0.3-0.5% of glycerin; 0.004-0.006% of glycerol polyacrylate; 0.0004 to 0.0006 percent of methylparaben; 0.0001-0.0003% of propyl hydroxybenzoate; 2-4% of ginseng and ganoderma lucidum polypeptide composition; 0.02-0.04% of caprylyl hydroximic acid; 0.03-0.05% of 1, 3-propylene glycol; 0.1 to 0.2 percent of butanediol; glycosphingolipids 0.03-0.06%; 0.4 to 0.5 percent of phenoxyethanol; 0.03-0.05% of ethylhexyl glycerol; 0.3 to 0.5 percent of essence;

the balance of water; the total mass percentage is 100 percent.

8. The application of the ginseng-ganoderma lucidum polypeptide composition for resisting wrinkles and tightening bodies is characterized in that: adding the ginseng ganoderma polypeptide composition of any one of claims 1-6 to milk, wherein the milk containing the ginseng ganoderma polypeptide composition comprises the following components in percentage by mass:

phase A: 2-4% of dioctyl carbonate; 1.5-2% of cyclopentasiloxane; 1-1.3% of cyclohexasiloxane; caprylic/capric triglyceride 1-3%; c14-22 alcohol 0.6-0.9%; 0.1-0.3% of C12-20 alkyl glucoside; 0.5-0.7% of polydimethylsiloxane; 0.1 to 0.3 percent of PEG-100 stearate; 0.2-0.4% of glycerol stearate; cetostearyl alcohol 0.2-0.5%; 0.1 to 0.4 percent of methyl hydroxybenzoate;

phase B: 2-4% of butanediol; 1-1.5% of propylene glycol; 0.1-0.3% of acryloyl dimethyl ammonium taurate/VP copolymer; 0.05 to 0.1 percent of allantoin; 0.06-0.09% of xanthan gum; 0.03-0.06% of EDTA disodium; 0.02 to 0.04 percent of sodium hyaluronate;

and C phase: 0.08 to 0.1 percent of water; 0.1-0.2% of sodium acrylate/sodium acryloyldimethyl taurate copolymer; 0.05 to 0.07 percent of isohexadecane; polysorbate-80.02-0.03%;

phase D: 1-1.5% of propylene glycol; 1-2% of water; 1-1.5% of chitosan glycolate; polyglycerol-10.5-1%; raspberry ketone glucoside 0.3-0.6%; carnosine 0.4-0.7%; 0.3 to 0.5 percent of p-hydroxyacetophenone; 0.5 to 0.8 percent of 1, 2-hexanediol; 3-5% of ginseng and ganoderma lucidum polypeptide composition; 0.04 to 0.05 percent of phenoxyethanol; 0.004-0.006% of ethylhexyl glycerol; 0.1 to 0.2 percent of essence;

phase E: CI 75470.0.0001-0.0002%; CI 19140.0001-0.0002%; CI 75100.0003-0.0005%;

the balance of water, the total mass percent is 100%.