CN115516083A - 从人经血中获得宫内膜间充质干细胞的方法 - Google Patents

从人经血中获得宫内膜间充质干细胞的方法 Download PDF

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CN115516083A
CN115516083A CN202180025074.9A CN202180025074A CN115516083A CN 115516083 A CN115516083 A CN 115516083A CN 202180025074 A CN202180025074 A CN 202180025074A CN 115516083 A CN115516083 A CN 115516083A
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cell
cell sieve
mesenchymal stem
sieve
tissue
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金槿
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Shanghai Iwu Stem Cell Technology Co ltd
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Shanghai Iwu Stem Cell Technology Co ltd
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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Abstract

提供了一种从人经血中获得宫内膜间充质干细胞的方法和由此制备的宫内膜间充质干细胞，所述方法包括：1)将经血样本分级过细胞筛以分离组织碎片，所述细胞筛的孔径逐级变小，包括用于收集滤过物的滤过细胞筛和用于收集截留物的截留细胞筛，截留细胞筛的孔径小于滤过细胞筛；2)收集截留细胞筛上的截留物即组织碎片；3)在间充质干细胞培养基中培养组织碎片；4)收集贴壁细胞即为宫内膜间充质干细胞。

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PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN202180025074.9A 2020-04-03 2021-04-02 从人经血中获得宫内膜间充质干细胞的方法 Pending CN115516083A (zh)

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CN2020102590456 2020-04-03
CN202010259045 2020-04-03
PCT/CN2021/085202 WO2021197459A1 (zh) 2020-04-03 2021-04-02 从人经血中获得宫内膜间充质干细胞的方法

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CN115516083A true CN115516083A (zh) 2022-12-23

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US (1) US20230116841A1 (zh)
EP (1) EP4130249A4 (zh)
JP (1) JP7428823B2 (zh)
CN (1) CN115516083A (zh)
WO (1) WO2021197459A1 (zh)

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* Cited by examiner, † Cited by third party
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WO2023178465A1 (zh) * 2022-03-21 2023-09-28 士泽生物医药(上海)有限公司 一种制备经血源性间充质干细胞的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
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JP5564679B2 (ja) 2009-04-16 2014-07-30 公益財団法人ヒューマンサイエンス振興財団 心筋細胞分化誘導促進剤およびその使用方法
CN103966162B (zh) 2014-05-29 2016-07-06 成都清科生物科技有限公司 一种经血来源间充质干细胞分离方法
CN109154614B (zh) 2016-03-18 2022-01-28 四方控股公司 用于细胞分离的组合物、装置和方法
CN108034634B (zh) * 2017-12-19 2021-01-12 杭州易文赛生物技术有限公司 一种从经血中分离宫内膜间充质干细胞的方法
CN108998411A (zh) * 2018-08-15 2018-12-14 山西医科大学 人子宫内膜组织来源间充质干细胞的分离培养方法
EP3848451A4 (en) 2018-09-11 2021-10-27 Nissan Chemical Corporation SEPARATION DEVICE AND PROCESS FOR SEPARATING MATERIAL TO BE SEPARATED BY THE USER
CN109182263A (zh) * 2018-09-20 2019-01-11 浙江卫未生物医药科技有限公司 一种用茶皂素溶解月经血红细胞分离宫膜间充质干细胞的方法
CN109845727B (zh) * 2019-03-25 2021-11-23 西安医斯美生物科技有限公司 经血保存液及经血来源间充质干细胞冻存方法

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EP4130249A4 (en) 2024-05-08
US20230116841A1 (en) 2023-04-13
EP4130249A1 (en) 2023-02-08
JP2023519371A (ja) 2023-05-10
JP7428823B2 (ja) 2024-02-06
WO2021197459A1 (zh) 2021-10-07

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