CN115485363A - 细胞悬液的制造方法和黏附细胞的制造方法 - Google Patents

细胞悬液的制造方法和黏附细胞的制造方法 Download PDF

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Publication number
CN115485363A
CN115485363A CN202080098424.XA CN202080098424A CN115485363A CN 115485363 A CN115485363 A CN 115485363A CN 202080098424 A CN202080098424 A CN 202080098424A CN 115485363 A CN115485363 A CN 115485363A
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cell suspension
adherent cells
cells
microcarriers
volume
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Chinese (zh)
Inventor
陈尚武
中川史子
多田靖彦
冈野定雅弘
佐藤优至
铃木悠午
中岛克彦
高桥亮介
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Resonac Holdings Corp
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Showa Denko KK
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202080098424.XA 2020-03-13 2020-12-22 细胞悬液的制造方法和黏附细胞的制造方法 Pending CN115485363A (zh)

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JP2020-043974 2020-03-13
JP2020043974 2020-03-13
PCT/JP2020/047981 WO2021181819A1 (ja) 2020-03-13 2020-12-22 細胞懸濁液の製造方法、及び、接着細胞の製造方法

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US (1) US20220315898A1 (enExample)
EP (1) EP3907278B8 (enExample)
JP (2) JP7767147B2 (enExample)
CN (1) CN115485363A (enExample)
WO (1) WO2021181819A1 (enExample)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
EP4230724A4 (en) * 2020-10-16 2024-05-22 FUJIFILM Corporation CULTURE MEDIUM FOR THE PRODUCTION OF EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE AND METHOD FOR THE PRODUCTION OF EXTRACELLULAR VESICLES
JP7160294B1 (ja) 2022-03-31 2022-10-25 ヒューマン・メタボローム・テクノロジーズ株式会社 脂質二重膜に覆われた微粒子又は細胞外小胞の回収方法および脂質二重膜に覆われた微粒子又は細胞外小胞を回収するための回収キット
WO2025183119A1 (ja) * 2024-02-29 2025-09-04 富士フイルム株式会社 間葉系幹細胞の製造方法
WO2025197916A1 (ja) * 2024-03-18 2025-09-25 大日本印刷株式会社 新規細胞培養方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151506A1 (en) * 2009-12-23 2011-06-23 Sanofi Pasteur Sa Process for Culturing Adherent Cells
CN102653728A (zh) * 2011-03-04 2012-09-05 北京清大天一科技有限公司 一种用生物反应器和微载体逐级放大培养动物细胞的方法
CN102827804A (zh) * 2012-08-30 2012-12-19 苏州市沃美生物技术有限公司 适用于Vero细胞微载体悬浮放大培养的培养基及方法
CN103614333A (zh) * 2013-11-15 2014-03-05 乔自林 一种用胶原微载体在反应器中扩大培养动物细胞的方法

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
DE102004049290A1 (de) * 2004-10-09 2006-04-20 Bayer Healthcare Ag Verfahren zur Herstellung von Virusmaterial
CN102014934B (zh) 2008-02-22 2014-08-20 新加坡科技研究局 间充质干细胞颗粒
JP2020043974A (ja) 2018-09-18 2020-03-26 京楽産業．株式会社 遊技機

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151506A1 (en) * 2009-12-23 2011-06-23 Sanofi Pasteur Sa Process for Culturing Adherent Cells
CN102653728A (zh) * 2011-03-04 2012-09-05 北京清大天一科技有限公司 一种用生物反应器和微载体逐级放大培养动物细胞的方法
CN102827804A (zh) * 2012-08-30 2012-12-19 苏州市沃美生物技术有限公司 适用于Vero细胞微载体悬浮放大培养的培养基及方法
CN103614333A (zh) * 2013-11-15 2014-03-05 乔自林 一种用胶原微载体在反应器中扩大培养动物细胞的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
林美玲等: "重组HSV—Ⅱ病毒疫苗的微载体悬浮培养生产工艺研究", 中国生物工程杂志, vol. 34, no. 3, 31 December 2014 (2014-12-31), pages 1 - 17 *

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US20220315898A1 (en) 2022-10-06
WO2021181819A1 (ja) 2021-09-16
EP3907278A4 (en) 2022-02-23
JPWO2021181819A1 (enExample) 2021-09-16
EP3907278A1 (en) 2021-11-10
EP3907278B1 (en) 2025-07-02
EP3907278B8 (en) 2025-08-27
JP7767147B2 (ja) 2025-11-11
JP2023134611A (ja) 2023-09-27

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