JP7767147B2 - 細胞懸濁液の製造方法、及び、接着細胞の製造方法 - Google Patents

細胞懸濁液の製造方法、及び、接着細胞の製造方法

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JP7767147B2
JP7767147B2 JP2021538009A JP2021538009A JP7767147B2 JP 7767147 B2 JP7767147 B2 JP 7767147B2 JP 2021538009 A JP2021538009 A JP 2021538009A JP 2021538009 A JP2021538009 A JP 2021538009A JP 7767147 B2 JP7767147 B2 JP 7767147B2
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cell suspension
adherent cells
cells
microcarriers
stirring
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JPWO2021181819A1 (enExample
JPWO2021181819A5 (enExample
Inventor
尚武 陳
史子 中川
靖彦 多田
雅弘 岡野定
優至 佐藤
悠午 鈴木
克彦 中島
亮介 高橋
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ミナリスアドバンストセラピーズ株式会社
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Priority to JP2023113824A priority Critical patent/JP2023134611A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021538009A 2020-03-13 2020-12-22 細胞懸濁液の製造方法、及び、接着細胞の製造方法 Active JP7767147B2 (ja)

Priority Applications (1)

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JP2023113824A JP2023134611A (ja) 2020-03-13 2023-07-11 細胞懸濁液の製造方法、及び、接着細胞の製造方法

Applications Claiming Priority (3)

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JP2020043974 2020-03-13
JP2020043974 2020-03-13
PCT/JP2020/047981 WO2021181819A1 (ja) 2020-03-13 2020-12-22 細胞懸濁液の製造方法、及び、接着細胞の製造方法

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JPWO2021181819A1 JPWO2021181819A1 (enExample) 2021-09-16
JPWO2021181819A5 JPWO2021181819A5 (enExample) 2022-03-08
JP7767147B2 true JP7767147B2 (ja) 2025-11-11

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JP2023113824A Withdrawn JP2023134611A (ja) 2020-03-13 2023-07-11 細胞懸濁液の製造方法、及び、接着細胞の製造方法

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Country Status (5)

Country Link
US (1) US20220315898A1 (enExample)
EP (1) EP3907278B8 (enExample)
JP (2) JP7767147B2 (enExample)
CN (1) CN115485363A (enExample)
WO (1) WO2021181819A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4230724A4 (en) * 2020-10-16 2024-05-22 FUJIFILM Corporation CULTURE MEDIUM FOR THE PRODUCTION OF EXTRACELLULAR VESICLES, CULTURE MEDIUM KIT, ADDITIVE AND METHOD FOR THE PRODUCTION OF EXTRACELLULAR VESICLES
JP7160294B1 (ja) 2022-03-31 2022-10-25 ヒューマン・メタボローム・テクノロジーズ株式会社 脂質二重膜に覆われた微粒子又は細胞外小胞の回収方法および脂質二重膜に覆われた微粒子又は細胞外小胞を回収するための回収キット
WO2025183119A1 (ja) * 2024-02-29 2025-09-04 富士フイルム株式会社 間葉系幹細胞の製造方法
WO2025197916A1 (ja) * 2024-03-18 2025-09-25 大日本印刷株式会社 新規細胞培養方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004049290A1 (de) * 2004-10-09 2006-04-20 Bayer Healthcare Ag Verfahren zur Herstellung von Virusmaterial
CN102014934B (zh) 2008-02-22 2014-08-20 新加坡科技研究局 间充质干细胞颗粒
CN107460154A (zh) * 2009-12-23 2017-12-12 赛诺菲巴斯德有限公司 用于培养贴壁细胞的方法
CN102653728A (zh) * 2011-03-04 2012-09-05 北京清大天一科技有限公司 一种用生物反应器和微载体逐级放大培养动物细胞的方法
CN102827804B (zh) * 2012-08-30 2019-02-12 苏州市沃美生物技术有限公司 适用于Vero细胞微载体悬浮放大培养的培养基及方法
CN103614333A (zh) * 2013-11-15 2014-03-05 乔自林 一种用胶原微载体在反应器中扩大培养动物细胞的方法
JP2020043974A (ja) 2018-09-18 2020-03-26 京楽産業．株式会社 遊技機

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Publication number Publication date
US20220315898A1 (en) 2022-10-06
WO2021181819A1 (ja) 2021-09-16
EP3907278A4 (en) 2022-02-23
CN115485363A (zh) 2022-12-16
JPWO2021181819A1 (enExample) 2021-09-16
EP3907278A1 (en) 2021-11-10
EP3907278B1 (en) 2025-07-02
EP3907278B8 (en) 2025-08-27
JP2023134611A (ja) 2023-09-27

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