CN115474562B - Artificial propagation method for indoor cement pond of whitebait - Google Patents
Artificial propagation method for indoor cement pond of whitebait Download PDFInfo
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- CN115474562B CN115474562B CN202210620474.0A CN202210620474A CN115474562B CN 115474562 B CN115474562 B CN 115474562B CN 202210620474 A CN202210620474 A CN 202210620474A CN 115474562 B CN115474562 B CN 115474562B
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- 239000004332 silver Substances 0.000 claims description 93
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 14
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- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 8
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- 229960001723 oxytocin Drugs 0.000 claims description 8
- 229930003270 Vitamin B Natural products 0.000 claims description 7
- 229930003427 Vitamin E Natural products 0.000 claims description 7
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 7
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- 231100000241 scar Toxicity 0.000 claims description 6
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- 210000001672 ovary Anatomy 0.000 claims 1
- 239000003488 releasing hormone Substances 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 16
- 230000001418 larval effect Effects 0.000 abstract 1
- 229940090044 injection Drugs 0.000 description 14
- 229910001220 stainless steel Inorganic materials 0.000 description 7
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- XNOPRXBHLZRZKH-MQYCRUOZSA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1C(CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-MQYCRUOZSA-N 0.000 description 6
- 229940082408 oxytocin injection Drugs 0.000 description 6
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- 239000005556 hormone Substances 0.000 description 5
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- 239000007788 liquid Substances 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the technical field of breeding of silver-drum fishes, in particular to an indoor cement pond artificial breeding method for silver-drum fishes, which comprises five steps of breeding fish selection, breeding fish cultivation, spawning induction of the breeding fish, fertilized egg hatching and larval fish cultivation, wherein the fertilization rate of the silver-drum fishes can be effectively improved by adopting a dry artificial insemination mode, and the spawning rate of the female silver-drum fishes can be effectively improved by manually injecting 15ug/kg luteinizing hormone LHRH and 1500IU/kg chorionic gonadotrophin HCG into the female silver-drum fishes, so that the artificial breeding cost is effectively reduced, and the large-scale artificial breeding is facilitated.
Description
Technical Field
The invention relates to the technical field of breeding of whitebait, in particular to an artificial breeding method of a whitebait indoor cement pond.
Background
The silver drum fish (multi-line butterfly fish) is a kind of wild salt fish, the maximum length of the fish can reach 40 cm, the fish is silvery white, a small amount of black spots are arranged on the body side, the fish has good ornamental value and high economic value, and in order to avoid damage to ecological environment caused by transitional fishing of the silver drum fish in the sea, artificial propagation of the silver drum fish is needed.
For this chinese patent publication No.: CN113661946a discloses an outdoor pond culture method for silver drum fish, comprising the following steps: (1) placing the initially hatched fries into an indoor cultivation pond for transitional cultivation (2) placing fries in a pond (3) placing fries into the pond 2d, and feeding the fries by mixing the branch horns and the copepods; when the body length of the fry is more than or equal to 10mm and 50-60 percent, the water level and the salinity are improved, low-protein microalgae powder is fed, and the water temperature is reduced at the cooling rate of 0.5 ℃/h during feeding; when the juvenile fish body length is more than or equal to 60mm, the water level and the salinity are increased, and the black soldier fly larva powder containing the compound vitamin is fed; when the length of the adult fish body is more than 60 percent and is more than or equal to 10cm, the fresh minced fish and the live shrimp are fed in a combined way, and the water level is increased; when the length of the adult fish body is more than or equal to 15cm, the water level is increased; the outdoor pond culture method for the silver drum fish can effectively reduce the chasing phenomenon and the morbidity of pond culture, and realize the outdoor artificial scale pond culture with high survival rate and low morbidity.
However, by adopting the method provided by the invention, although the survival rate of the silver drum fish can be improved, the spawning rate and the fertilization rate of the silver drum fish are not effectively improved, so that the artificial propagation cost of the silver drum fish is high, and the large-scale artificial culture is not facilitated.
Therefore, there is a need to design an artificial propagation method for a cement pond in a silver drum fish room to solve the above problems.
Disclosure of Invention
The invention aims to provide an artificial propagation method for a silver drum fish in an indoor cement pond, which aims to solve the problem that the spawning rate and the fertilization rate of the silver drum fish cannot be effectively improved when the silver drum fish is cultured in an outdoor pond, which is provided by the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: an artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
an artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver-drum fish with the length specification of 11-13 cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 17-19 cm in the ocean for 7-8 months as a female parent;
s2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation;
(2) Paving an inclined mud layer on the bottom of the female parent cement pond, wherein the thickness of the mud layer is 10cm;
(3) The female parent cement pool is provided with a water inlet and a water outlet, and a booster pump is arranged at the water inlet;
(4) The salinity of the two constant temperature cement ponds is controlled to be 24-29 per mill;
(5) Vitamin B and vitamin E are added into both constant temperature cement ponds;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) The larva fish is hatched out of the film and fed with the rotifer bait on the third day, and the larva fish is fed with the artemia bait on the tenth day.
Preferably, in the step S1 (1), the top end of the head of the male silver drum fish is wrinkled.
Preferably, in the step S1 (2), the top end of the head of the female silver drum fish is smooth.
Preferably, the temperature of the cement pond in the step S2 (1) is 18-22 ℃ in winter and 28-32 ℃ in summer.
Preferably, an aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L.
Preferably, the selection criteria of the male parent silver drum fish in the step S3 are no disease, no surface scars, bright color, rapid response, milky white semen extruded from the abdomen, and immediate dispersion after water entering.
Preferably, the female parent silver drum fish selection standard in the step (2) is that the abdomen is tapped and ovarian shake is seen.
Preferably, the oxytocin is injected twice, the first 15ug/kg luteinizing hormone releasing hormone LHRH and the second 1500IU/kg chorionic gonadotrophin HCG, the first and second time being separated by 18 hours.
Preferably, at the time of the second injection of the female parent, 7.5ug/kg luteinizing hormone releasing hormone LHRH, and 7500IU/kg chorionic gonadotrophin HCG are injected into the male parent.
Preferably, in the step S3 (3), dry insemination is performed.
Compared with the prior art, the invention has the beneficial effects that: according to the artificial propagation method for the indoor cement ponds of the whitebait, artificial insemination is adopted, and hormone is injected into the whitebait, so that the spawning rate and the fertilization rate of the whitebait can be effectively improved, the cost of artificial propagation is effectively reduced, and large-scale artificial culture is facilitated.
(1) The fertilization rate of the silver drum fish can be effectively improved by adopting a dry artificial insemination mode.
(2) According to the invention, 15ug/kg luteinizing hormone-releasing hormone LHRH and 1500IU/kg chorionic gonadotrophin HCG are manually injected into female silver drum fish, so that the spawning rate of female silver drum fish can be effectively improved.
Drawings
FIG. 1 is a table showing the relationship between insemination mode and fertilization rates in example 3 of the present invention;
FIG. 2 is a table showing the relationship between luteinizing hormone releasing hormone, chorionic gonadotrophin and fertilization rates in example 5 of the present invention;
FIG. 3 is a table showing the relationship between luteinizing hormone releasing hormone, chorionic gonadotrophin and oviposition rate in example 5 of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides an embodiment:
example 1
An artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver-drum fish with the length specification of 11-13 cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 17-19 cm in the ocean for 7-8 months as a female parent;
according to the actual propagation condition of the silver-drum fish in the ocean, the situation that the male silver-drum fish is mostly mature in the period of 6 months can be found, but the female silver-drum fish is only partially mature, the large-scale production requirement is not met, and the mature eggs of the female silver-drum fish in the period of 7-8 months are the most, so that the silver-drum fish in the period of 7-8 months is selected as the breeding fish.
S2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation, wherein the optimal month of reproduction of the silver drum fish is 7 or 8 months, and the maturity of the female silver drum fish is highest at the moment, and the female silver drum fish and the male silver drum fish are required to be cultivated separately due to the asynchronism of the male and female gonad development, so that natural reproduction of male and female fish can be reduced, the excitement of the female silver drum fish fingerling and the male silver drum fish fingerling in the reproduction period can be increased, and the spawning rate and the fertilization rate of the silver drum fish can be improved;
(2) And paving an inclined mud layer at the bottom of the female parent cement pond, wherein the thickness of the mud layer is 10cm, and providing a powerful egg particle attachment place for female silver drum fishes by paving the mud layer.
(3) The female parent cement pond is provided with a water inlet and a water outlet, and the water inlet is provided with a booster pump, so that the female whitebait needs dynamic water flow when spawning and the characteristics of the female whitebait can cause the female whitebait to ovulate in a countercurrent way, and the water inlet is provided with the booster pump so as to accelerate the water flow flowing into the cement pond to form dynamic water flow and provide ovulation conditions for the female whitebait;
(4) The salinity of the two constant temperature cement ponds is controlled to be 24-29 per mill;
(5) Vitamin B and vitamin E are added into both constant-temperature cement ponds, and by adding vitamin B and vitamin E into the baits of the male parent and female parent whitebait, the physique of the whitebait can be effectively improved, the gonad development of the male parent and female parent whitebait can be promoted, and the fertility of the whitebait can be effectively enhanced;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) The larva fish is hatched out of the film and fed with the rotifer bait on the third day, and the larva fish is fed with the artemia bait on the tenth day.
In the step S1, the top end of the head of the male silver drum fish is wrinkled.
And (2) smoothing the top end of the head of the female silver drum fish in the step S1.
The smoothness of the top end of the head of the silver drum fish is observed to rapidly distinguish female silver drum fish and male silver drum fish, so that the artificial propagation efficiency is improved.
In the step S2, the temperature of the cement pond (1) is 18-22 ℃ in winter and 28-32 ℃ in summer.
An aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L.
The selection standard of the male parent silver drum fish in the step S3 is that no disease exists, no surface scars exist on the surface, the color is bright, the reaction is rapid, the milky white semen flows out after the abdomen is extruded, and the milky white semen is immediately dispersed after water is introduced.
And (3) the female parent silver drum fish selection standard in the step (2) is that the abdomen is tapped and ovarian shake is seen.
The oxytocin injection was twice, the first 15ug/kg luteinizing hormone releasing hormone LHRH, the second 1500IU/kg chorionic gonadotrophin HCG, the first and second time intervals being 18 hours.
During the second injection of the female parent, 7.5ug/kg luteinizing hormone LHRH and 7500IU/kg chorionic gonadotrophin HCG are injected into the male parent, and the male parent silver drum fish dysplasia can be avoided during artificial propagation, and at the moment, the male parent silver drum fish seminiferous can be effectively accelerated by adopting a method of injecting the hormone into the male parent.
In the step S3, (3) dry insemination is adopted, female silver-drum fishes which are fully mature and about to ovulate or have started to ovulate are fished out, water drops on the body surface are wiped off by a towel, the hands are kept dry, the abdomen of the female silver-drum fishes is lightly extruded to enable eggs to flow out, the female silver-drum fishes are placed into a sterilized dry stainless steel basin which is prepared in advance, then the male silver-drum fishes are fished out and the body surface of the female silver-drum fishes is wiped off, moisture of a drain hole is extruded out, semen is poured into the dry stainless steel basin, stirring is carried out uniformly, then 20 perming of sea water is added into the dry stainless steel basin, stirring is carried out for 5 minutes to achieve the fertilization purpose of sperms and eggs of the silver-drum fishes, then the mixed liquid of sperms and eggs in the stainless steel basin is rinsed for a plurality of times until the mixed liquid is clear, and finally the mixed liquid is poured into a hatching pond to hatch.
Example 2
An artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver drum fish with the length specification of 12cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 18cm in the ocean for 7-8 months as a female parent;
s2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation;
(2) Paving an inclined mud layer on the bottom of the female parent cement pond, wherein the thickness of the mud layer is 15cm;
(3) The female parent cement pool is provided with a water inlet and a water outlet, and a booster pump is arranged at the water inlet;
(4) The salinity of the two constant-temperature cement ponds is controlled to be 27 per mill;
(5) Vitamin B and vitamin E are added into both constant temperature cement ponds;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) The larva fish is hatched out of the film and fed with the rotifer bait on the third day, and the larva fish is fed with the artemia bait on the tenth day.
In the step S1, the top end of the head of the male silver drum fish is wrinkled.
And (2) smoothing the top end of the head of the female silver drum fish in the step S1.
In the step S2, the temperature of the cement pond in the winter is 22 ℃ and the temperature in the summer is 32 ℃.
An aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L.
The selection standard of the male parent silver drum fish in the step S3 is that no disease exists, no surface scars exist on the surface, the color is bright, the reaction is rapid, the milky white semen flows out after the abdomen is extruded, and the milky white semen is immediately dispersed after water is introduced.
And (3) the female parent silver drum fish selection standard in the step (2) is that the abdomen is tapped and ovarian shake is seen.
The oxytocin injection was twice, the first 15ug/kg luteinizing hormone releasing hormone LHRH, the second 1500IU/kg chorionic gonadotrophin HCG, the first and second time intervals being 18 hours.
At the time of the second injection of the female parent 7.5ug/kg luteinizing hormone releasing hormone LHRH, and 7500IU/kg chorionic gonadotrophin HCG were injected into the male parent.
In the step S3, (3) semidry insemination is adopted, female silver-drum fishes which are fully mature and about to ovulate or have started to ovulate are fished out, water drops on the body surface are wiped by a towel, hands are kept dry, the abdomen of the female silver-drum fishes is gently squeezed to enable eggs to flow out, the female silver-drum fishes are placed into a sterilized dry stainless steel basin which is prepared in advance, male silver-drum fishes are fished out and wiped to dry the body surface of the male silver-drum fishes, water in a drain hole is squeezed out, semen is poured into a beaker containing 0.85% normal saline to be diluted, the semen is poured into the dry stainless steel basin to be uniformly stirred, and finally 20 permillage of seawater is added into the dry stainless steel basin to be stirred for 5 minutes.
Example 3
An artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver-drum fish with the length specification of 11-13 cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 17-19 cm in the ocean for 7-8 months as a female parent;
s2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation;
(2) Paving an inclined mud layer on the bottom of the female parent cement pond, wherein the thickness of the mud layer is 10cm;
(3) The female parent cement pool is provided with a water inlet and a water outlet, and a booster pump is arranged at the water inlet;
(4) The salinity of the two constant temperature cement ponds is controlled to be 24-29 per mill;
(5) Vitamin B and vitamin E are added into both constant temperature cement ponds;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) The larva fish is hatched out of the film and fed with the rotifer bait on the third day, and the larva fish is fed with the artemia bait on the tenth day.
In the step S1, the top end of the head of the male silver drum fish is wrinkled.
And (2) smoothing the top end of the head of the female silver drum fish in the step S1.
In the step S2, the temperature of the cement pond (1) is 18-22 ℃ in winter and 28-32 ℃ in summer.
An aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L.
The selection standard of the male parent silver drum fish in the step S3 is that no disease exists, no surface scars exist on the surface, the color is bright, the reaction is rapid, the milky white semen flows out after the abdomen is extruded, and the milky white semen is immediately dispersed after water is introduced.
And (3) the female parent silver drum fish selection standard in the step (2) is that the abdomen is tapped and ovarian shake is seen.
The oxytocin injection was twice, the first 15ug/kg luteinizing hormone releasing hormone LHRH, the second 1500IU/kg chorionic gonadotrophin HCG, the first and second time intervals being 18 hours.
At the time of the second injection of the female parent 7.5ug/kg luteinizing hormone releasing hormone LHRH, and 7500IU/kg chorionic gonadotrophin HCG were injected into the male parent.
In the step S3 (3), wet insemination is adopted, the roe and the semen are simultaneously extruded into a basin containing 20 per mill of seawater, and the mixture is stirred for 5 minutes.
According to the artificial propagation method of the indoor cement pond of the silver drum fish in the embodiments 1-3, different artificial insemination modes of the silver drum fish are carried out, and as can be seen from the accompanying figure 1 in the specification, the effect of adopting a dry fertilization mode on the silver drum fish is optimal, generally, the effective fertilization time of healthy ova is about 1 hour, the ova can be deactivated after leaving the protection of ovarian liquid, the sperms can be deactivated very quickly in water, the coincidence of the egg output and the emission of the sperms in time is the premise of ensuring that a plurality of ova can be fertilized, and under the condition that the female silver drum fish and the male silver drum fish are not fully synchronous in maturation and the insemination of the male silver drum fish can not be ensured immediately after the female silver drum fish spawns, the fertilization rate of the silver drum fish can be effectively improved by adopting the artificial insemination mode.
Example 4
An artificial propagation method of a silver drum fish indoor cement pond comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver-drum fish with the length specification of 11-13 cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 17-19 cm in the ocean for 7-8 months as a female parent;
s2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation;
(2) Paving an inclined mud layer on the bottom of the female parent cement pond, wherein the thickness of the mud layer is 10cm;
(3) The female parent cement pool is provided with a water inlet and a water outlet, and a booster pump is arranged at the water inlet;
(4) The salinity of the two constant temperature cement ponds is controlled to be 24-29 per mill;
(5) Vitamin B and vitamin E are added into both constant temperature cement ponds;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) The larva fish is hatched out of the film and fed with the rotifer bait on the third day, and the larva fish is fed with the artemia bait on the tenth day.
In the step S1, the top end of the head of the male silver drum fish is wrinkled.
And (2) smoothing the top end of the head of the female silver drum fish in the step S1.
In the step S2, the temperature of the cement pond (1) is 18-22 ℃ in winter and 28-32 ℃ in summer.
An aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L.
The selection standard of the male parent silver drum fish in the step S3 is that no disease exists, no surface scars exist on the surface, the color is bright, the reaction is rapid, the milky white semen flows out after the abdomen is extruded, and the milky white semen is immediately dispersed after water is introduced.
And (3) the female parent silver drum fish selection standard in the step (2) is that the abdomen is tapped and ovarian shake is seen.
The oxytocin injection was twice, the first injection of 20ug/kg luteinizing hormone releasing hormone LHRH and the second injection of 1500IU/kg chorionic gonadotrophin HCG, the first and second time intervals being 18 hours.
At the time of the second injection of the female parent 7.5ug/kg luteinizing hormone releasing hormone LHRH, and 7500IU/kg chorionic gonadotrophin HCG were injected into the male parent.
In the step S3, (3) dry insemination is adopted.
Example 4 differs from example 1 in that the oxytocin injection of example 3 was performed twice, the first injection of 20ug/kg luteinizing hormone releasing hormone LHRH and the second injection of 1500IU/kg chorionic gonadotropin HCG, the first and second time being separated by 18 hours.
Example 5
Example 5 differs from example 1 in that the oxytocin injection of example 4 was performed twice, the first injection of 10ug/kg luteinizing hormone releasing hormone LHRH and the second injection of 1000IU/kg chorionic gonadotropin HCG, the first and second time being separated by 18 hours.
According to the artificial propagation methods of the cement sheath in the silver-colored fish room of the above-mentioned examples 1, 4 and 5, the injection experiments of different hormone injection amounts of silver-colored fish were carried out, and it can be seen from FIG. 3 of the specification that when luteinizing hormone LHRH of 10 to 20ug/kg was injected for the first time, luteinizing hormone HCG of 1000 to 2000IU/kg was injected for the second time at 18 hours, the egg laying rate was 100%, when luteinizing hormone LHRH of 5 to 10ug/kg was injected for the first time, luteinizing hormone HCG of 500 to 1000IU/kg was injected for the second time at 18 hours, the egg laying rate was 75.37%, when luteinizing hormone LHRH of 1 to 5ug/kg was injected for the first time, luteinizing hormone HCG of 1 to 500IU/kg was injected for the second time at 18 hours, and the egg laying rate was only 20%, and the combination of different hormone dosages also had the difference, as can be seen from the attached figure 2 of the specification, the effect of injecting 15ug/kg of luteinizing hormone LHRH and 1500IU/kg of chorionic gonadotrophin HCG is best, the fertilization rate reaches 92.36%, the effect of injecting 17ug/kg of luteinizing hormone LHRH and 1500IU/kg of chorionic gonadotrophin HCG is reduced, the fertilization rate is 83.52%, by comprehensively comparing the induced spawning rates of hormones with different dosages, combinations and proportions, a stable and relatively economical induced spawning scheme is established, namely, under the condition of 28-30 ℃, 15ug/kg of luteinizing hormone LHRH is manually injected, after 18 hours, the fertilization rate of the silver drum fish reaches 100%, and the fertilization rate reaches more than 70% by adopting a dry artificial insemination mode, meanwhile, as the male silver-drum fish does not generally need artificial spawning promotion and maturation promotion, only when the second female silver-drum fish injection is carried out, if the male silver-drum fish is bad in development, half of the dosage of the female silver-drum fish can be properly injected to accelerate the semen discharge, and the success rate of the spawning promotion method reaches 95% through multiple production verification.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (1)
1. An artificial propagation method for a silver drum fish indoor cement pond is characterized by comprising the following steps of: the method comprises the following steps:
s1, breeding fish selection
(1) Selecting male silver-drum fish with the length specification of 11-13 cm in the ocean for 7-8 months as a male parent;
(2) Selecting female silver drum fish with the length specification of 17-19 cm in the ocean for 7-8 months as a female parent;
s2, breeding of the breeding fish
(1) Respectively placing the male parent and the female parent into two indoor cement ponds for cultivation;
(2) Paving an inclined mud layer on the bottom of the female parent cement pond, wherein the thickness of the mud layer is 10cm;
(3) The female parent cement pool is provided with a water inlet and a water outlet, and a booster pump is arranged at the water inlet;
(4) The salinity of the two constant temperature cement ponds is controlled to be 24-29 per mill;
(5) Vitamin B and vitamin E are added into both constant temperature cement ponds;
s3, spawning induction of the breeding fish
(1) Selecting silver drum fish with a body weight of more than 250g from a male parent cement pond;
(2) Selecting silver drum fish with a body weight of more than 650g from a female parent cement pond, and injecting oxytocin into the silver drum fish;
(3) Artificial insemination;
(4) Collecting fertilized eggs and sending the fertilized eggs into a hatching pond;
s4, hatching fertilized eggs
The salinity of the hatching pond is controlled at 21 per mill, the water temperature is 29 ℃, and the dissolved oxygen amount is 10mg/L;
s5, breeding of fries
(1) Controlling the temperature of the hatching pond to be 28 ℃, controlling the salinity to be 20 per mill, controlling the PH value to be 7.8, and controlling the dissolved oxygen to be 5mg/L;
(2) Feeding rotifer baits on the third day when the larvae hatch out of the film, and feeding artemia baits on the tenth day;
in the step S1, (1) the top end of the head of the male silver drum fish is wrinkled;
in the step S1, (2) the top end of the head of the female silver drum fish is smooth;
in the step S2, the temperature of the cement pond is 18-22 ℃ in winter and 28-32 ℃ in summer;
an aerator is arranged in the cement pool, and the oxygen content of the cement pool is controlled to be 5 mg/L-10 mg/L;
the selection standard of the male parent silver drum fish in the step S3 is that no disease exists, no surface scars exist on the surface, the color is bright, the reaction is rapid, the milky semen flows out after the abdomen is extruded, and the milky semen is immediately dispersed after water is introduced;
the female parent silver drum fish in the step S3 has the selection standard that the ovaries shake when the abdomen is tapped;
the oxytocin is injected twice, 15ug/kg luteinizing hormone is injected for releasing hormone LHRH for the first time, 1500IU/kg chorionic gonadotrophin HCG is injected for the second time, and the time interval between the first time and the second time is 18 hours; at the time of the second injection of the female parent, 7.5ug/kg luteinizing hormone releasing hormone LHRH, and 7500IU/kg chorionic gonadotrophin HCG are injected into the male parent;
in the step S3, (3) dry insemination is adopted.
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| KR20200092089A (en) * | 2019-01-24 | 2020-08-03 | 우영수 | Fish cultivation farm easy to feed |
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| CN113661946A (en) * | 2021-07-30 | 2021-11-19 | 陵水晨海种业有限公司 | Outdoor pond culture method for silver drum fish |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104663547A (en) * | 2015-03-11 | 2015-06-03 | 中国水产科学研究院长江水产研究所 | Percocypris scaled artificial propagation method |
| CN106172136A (en) * | 2016-07-26 | 2016-12-07 | 江苏中洋集团股份有限公司 | A kind of Puffer fish artificial fecundation method in winter |
| KR20190050435A (en) * | 2017-11-03 | 2019-05-13 | 부경대학교 산학협력단 | A method of farming of sablefis through regulation of density |
| KR20200092089A (en) * | 2019-01-24 | 2020-08-03 | 우영수 | Fish cultivation farm easy to feed |
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| CN113615608A (en) * | 2021-08-31 | 2021-11-09 | 陵水晨海种业有限公司 | Artificial breeding method of salangid |
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