CN115466667A

CN115466667A - Device and method for extracting bacteria microorganism detection sample liquid

Info

Publication number: CN115466667A
Application number: CN202211294081.1A
Authority: CN
Inventors: 刘圣; 梁健; 贾承霖; 周旋
Original assignee: Zhonghuanlianxin Beijing Environmental Protection Co ltd
Current assignee: Zhonghuanlianxin Beijing Environmental Protection Co ltd
Priority date: 2022-10-21
Filing date: 2022-10-21
Publication date: 2022-12-13
Anticipated expiration: 2042-10-21
Also published as: CN115466667B

Abstract

The invention discloses a bacteria microorganism detection sample liquid extraction device, which comprises a tube body and a grinding and filtering mechanism arranged in the inner cavity of the tube body, wherein the grinding and filtering mechanism comprises a rotating shaft rod which is vertically arranged in the tube body and can rotate in a reciprocating manner under the action of external drive; grind on the rotatory axostylus axostyle is connected to the axial direction of mechanism edge rotatory axostylus axostyle, grind the mechanism including extrusion seat and grinding seat, extrusion seat and rotatory axostylus axostyle screwed connection, grind seat and rotatory axostylus axostyle looks rigid coupling, the extrusion seat is along rotatory axostylus axostyle reciprocating motion in order to be close to or keep away from the grinding seat under the reciprocating rotation effect of rotatory axostylus axostyle, it follows the synchronous rotation of rotatory axostylus axostyle to grind the seat under the reciprocating rotation effect of rotatory axostylus axostyle, the axial direction fixed connection of rotatory axostylus axostyle is followed to the filter screen, adopt the mode of the rotatory extrusion grinding of single commentaries on classics to grind out, thereby the residue discharge of row cinder notch under the effect of centrifugal force after just grinding is discharged, it is convenient to arrange the material, simultaneously under the filtration of filter screen further the content of residue in the sample liquid.

Description

Device and method for extracting bacteria microorganism detection sample liquid

Technical Field

The invention relates to the technical field of extraction devices, in particular to a device and a method for extracting a bacteria microorganism detection sample solution.

Background

At present, in the biological medicine field, the fungus is like tremella, glossy ganoderma, hedgehog fungus etc. because of it has the edible property, by using in a large number as the raw and other materials of medicine, before carrying out the production preparation, generally need carry out the detection and analysis to the fungus earlier, survey its composition, generally need grind the fungus class sample when detecting and smash, extract the appearance liquid and carry out assay analysis, traditional mode is that the mode that adopts the sword to cut is broken with the sample, the appearance liquid wherein is extracted with the sample grinding through the mode of grinding, it is comparatively troublesome to adopt this kind of mode operation.

Although there is also the possibility that the filter screen is arranged in the extraction device for filtering so as to reduce the blockage of the extraction pipe, the grinding seat of the device and the residues on the filter screen are not easy to clean, the whole device needs to be disassembled during cleaning, the grinding seat and the filter screen are disassembled and cleaned, frequent extraction and detection are not facilitated, the detection efficiency is low, and frequent disassembly and assembly easily cause the phenomenon of leakage due to the fact that the joint is not tightly attached, and therefore the device and the method for extracting the bacteria microorganism detection sample liquid are provided.

Disclosure of Invention

The invention aims to provide a device and a method for extracting a bacteria microorganism detection sample solution, which aim to solve the problems in the background technology.

In order to solve the technical problems, the invention specifically provides the following technical scheme:

the utility model provides a fungus class microorganism detects appearance liquid extraction element, includes the body and installs the grinding filter mechanism of body inner chamber, grinding filter mechanism includes:

the rotating shaft rod is vertically arranged in the pipe body and can rotate in a reciprocating manner under the action of external drive;

the grinding mechanism is connected to the rotating shaft rod along the axial direction of the rotating shaft rod and comprises an extrusion seat and a grinding seat, the extrusion seat is in threaded connection with the rotating shaft rod and is fixedly connected with the rotating shaft rod, the extrusion seat moves back and forth along the rotating shaft rod under the action of the reciprocating rotation of the rotating shaft rod so as to be close to or far away from the grinding seat, and the grinding seat rotates synchronously along the rotating shaft rod under the action of the reciprocating rotation of the rotating shaft rod so as to grind fungi and throw away fungi residues through a pipeline in the pipe body under the action of the centrifugal force of the grinding seat;

the filter screen is fixedly connected with the axial direction of the rotating shaft rod, and the filter screen synchronously rotates along with the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod so as to filter and separate sample liquid penetrating through the grinding mechanism.

As a preferred scheme of the invention, the rotating shaft rod comprises a threaded section and a smooth section, the threaded section is in threaded connection with the extrusion seat, and the smooth section is fixedly connected with the grinding seat and the filter screen;

the side walls of the pipe body, which are positioned on two sides of the threaded section, are symmetrically provided with two guide grooves, the side wall of the extrusion seat is provided with guide blocks which are in sliding connection with the guide grooves, and the extrusion seat moves along the guide grooves to be close to or far away from the grinding seat under the rotation action of the rotating shaft rod.

As a preferred scheme of the invention, the extrusion seat and the grinding seat have the same shape, and the edges of the extrusion seat and the grinding seat are both perpendicular to the inner cavity side wall of the tube body;

the extrusion seat comprises a first funnel seat and a first circular ring seat, the circumferential outer side wall of the first funnel seat is fixedly connected with the first circular ring seat, the first funnel seat is in threaded connection with the thread section, and a guide block arranged on the first circular ring seat reciprocates along the guide groove under the rotation action of the rotating shaft rod;

the grinding seat comprises a second funnel seat and a second circular ring seat, one end of the circumferential outer side wall of the second circular ring seat is fixedly connected with the second funnel seat, the other end of the second circular seat is inserted into a sliding groove in the side wall of the pipe body, and the second funnel seat is fixedly connected with the smooth section;

and liquid guide holes are formed in the inner cavities of the second funnel seat and the second circular ring seat, so that the grinding liquid in the grinding seat flows out from the liquid guide holes.

As a preferable scheme of the present invention, the liquid guiding hole is obliquely arranged, and an included angle between the liquid guiding hole and the end surfaces of the second funnel seat and the second annular seat is an acute angle.

As a preferred scheme of the invention, a feed inlet is formed in the side wall of the pipe body, and the feed inlet is positioned between the grinding seat and the extrusion seat;

the bottom of the pipe body is of a circular arc-shaped structure which is sunken mutually, and a discharge hole is formed in the center of the circular arc-shaped structure, so that sample liquid separated from the filter screen is discharged from the discharge hole.

As a preferable scheme of the present invention, a slag discharge port is provided on a side wall of the pipe body directly above the grinding seat and the filter screen, and residues on the grinding seat and the filter screen are discharged from the slag discharge port under the centrifugal force action of the rotating shaft rod.

As a preferred scheme of the present invention, the filter screen is arranged in a funnel-shaped structure, and the filter screen is fixedly connected to the bottom end of the rotating shaft rod, so that the residue is collected at the edge of the filter screen under the centrifugal force action of the filter screen.

As a preferable scheme of the invention, one end of the extrusion seat close to the grinding seat is provided with saw teeth 8, so that the extrusion seat can grasp bacterial microorganisms.

In a preferred embodiment of the present invention, a method for using the fungus microorganism detection sample liquid extraction device includes:

s100, fungus is placed into the filter screen from the feed inlet, after the fungus is placed into the filter screen, the outer driving mechanism is started, the extrusion seat is driven by the rotating shaft rod to be close to the grinding seat, fungus cells are crushed under the grinding effect of the grinding seat, sample liquid is filtered out under the separation of the filter screen, and the sample liquid is collected through the discharge outlet.

S200, after the sample liquid is collected, the external driving mechanism rotates reversely, and residues on the grinding seat and the filter screen are discharged from a residue discharge port through the centrifugal force of the rotating shaft rod.

Compared with the prior art, the invention has the following beneficial effects:

the liquid outlet device adopts a single-rotation rotary extrusion grinding mode to grind liquid, and ground residues are discharged from the residue discharge port under the action of centrifugal force, the whole device does not need to be dismantled, the residue discharge is convenient and quick, the device is suitable for continuous extraction and detection, the detection efficiency is high, and the content of the residues in the sample liquid is further reduced under the filtration of the filter screen.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary and that other implementation drawings may be derived from the provided drawings by those of ordinary skill in the art without inventive effort.

FIG. 1 is a front view of the whole structure of the device provided by the present invention;

FIG. 2 is a cross-sectional side view of the overall structure of the device provided by the present invention;

the reference numerals in the drawings denote the following, respectively:

1. a pipe body; 2. rotating the shaft lever; 3. a grinding mechanism; 4. a filter screen; 5. a feed inlet; 6. a discharge port; 7. a slag discharge port; 8. saw teeth;

21. a threaded section; 22. a smooth section; 23. a guide groove; 24. a guide block;

31. a pressing base; 32. a grinding seat; 33. a first funnel base; 34. a first annular seat; 35. a second funnel base; 36. a second annular seat; 37. and a liquid guide hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1-2, a fungus microorganism detection sample liquid extraction device, includes body 1 and installs the grinding filter mechanism at body 1 inner chamber, and grinding filter mechanism includes:

the rotating shaft lever 2 is vertically arranged in the pipe body 1 and can rotate in a reciprocating manner under the action of external drive;

the grinding mechanism 3 is connected to the rotating shaft rod 2 along the axial direction of the rotating shaft rod 2 and comprises an extrusion seat 31 and a grinding seat 32, the extrusion seat 31 is in threaded connection with the rotating shaft rod 2, the grinding seat 32 is fixedly connected with the rotating shaft rod 2, the extrusion seat 31 reciprocates along the rotating shaft rod 2 under the reciprocating rotation action of the rotating shaft rod 2 to be close to or far away from the grinding seat 32, the grinding seat 32 rotates synchronously along the rotating shaft rod 2 under the reciprocating rotation action of the rotating shaft rod 2 to grind fungi, and fungi residues are thrown out through a pipeline in the pipe body 1 under the centrifugal force action of the grinding seat 32;

the filter screen 4, along the axial direction fixed connection of rotating shaft 2, the filter screen 4 follows rotating shaft 2 synchronous rotation under rotating shaft 2's reciprocating rotation effect to the sample liquid that sees through filters the separation in messenger grinding mechanism 3.

The device grinds mechanism and filter mechanism simultaneous movement through single rotatory axostylus axostyle drive, and outside grinding the centrifugal force effect of mechanism and filter mechanism with the residue discharge pipe, for current extraction element, this device need not dismounting device and carries out the residue and handles, and grinds effectually.

Wherein, it can be understood that the outer drive power of rotatory axostylus axostyle can be the motor, or be the rotation handle, and preferred mode is the motor, and degree of automation is high, can improve sample liquid extraction efficiency.

Specifically, as shown in fig. 2, the rotating shaft 2 includes a threaded section 21 and a smooth section 22, the threaded section 21 is in threaded connection with the extrusion seat 31, and the smooth section 22 is fixedly connected with the grinding seat 32 and the filter screen 4;

two guide grooves 23 are symmetrically arranged on the side walls of the pipe body 1 on two sides of the threaded section 21, guide blocks 24 which are in sliding connection with the guide grooves 23 are arranged on the side walls of the extrusion seat 31, and the extrusion seat 31 moves along the guide grooves 23 to be close to or far away from the grinding seat 32 under the rotating action of the rotating shaft rod 2.

The rotation drive extrusion seat 31 through rotatory axostylus axostyle 2 rotates along screw thread section 21 and goes up and down, because the guide block 24 of extrusion seat 31 lateral wall is connected with the guide way 23 cooperation on the body 1, consequently under the effect of rotation of rotatory axostylus axostyle 2, extrusion seat 31 only can be along the slip from top to bottom of rotatory axostylus axostyle 2, and grinds seat 32 and rotatory axostylus axostyle 2 simultaneous movement, utilizes to grind seat 32 and grinds the fungus class microorganism.

The residue is discharged and polished by centrifugal force, so that the residue remaining on the polishing pad 32 is reduced and the polished slurry is collected.

Specifically, as shown in fig. 1-2, the extrusion seat 31 and the grinding seat 32 have the same shape, and the edges of the extrusion seat 31 and the grinding seat 32 are perpendicular to the inner cavity sidewall of the tube 1;

the extrusion seat 31 comprises a first funnel seat 33 and a first circular ring seat 34, the circumferential outer side wall of the first funnel seat 33 is fixedly connected with the first circular ring seat 34, the first funnel seat 33 is in threaded connection with the threaded section 21, and the guide block 24 arranged on the first circular ring seat 34 reciprocates along the guide groove 23 under the rotation action of the rotating shaft rod 2;

the grinding seat 32 comprises a second funnel seat 35 and a second circular seat 36, one end of the circumferential outer side wall of the second circular seat 36 is fixedly connected with the second funnel seat 35, the other end of the second circular seat is inserted into a sliding groove in the side wall of the pipe body 1, and the second funnel seat 35 is fixedly connected with the smooth section 22;

the inner cavities of the second funnel seat 35 and the second circular ring seat 36 are both provided with a liquid guiding hole 37, so that the grinding liquid in the grinding seat 32 flows out from the liquid guiding hole 37.

The extrusion seat 31 and the grinding seat 32 are extruded in a matching way, the inclined second funnel seat 35 facilitates the sliding of the bacteria microorganism, the bacteria microorganism on the second funnel seat 35 is gathered on the second circular seat 36 under the centrifugal force of the rotating shaft rod 2, and is ground under the extrusion of the first funnel seat 33 and the first circular seat 34,

wherein the fungus is extruded all the time to the in-process that descends to first funnel seat 33 and first ring seat 34 to make the fungus extrude all the time on grinding seat 32, with the fast revolution of grinding seat 32 in order to grind the fungus, the sap that grinds out flows out along drain hole 37.

Because the extrusion seat 31 vertically extrudes the grinding seat 32 downwards, the force applied by the extrusion seat 31 also vertically acts on fungi, and residues of the fungi can be extruded into the liquid guide hole 37 under the action of the vertical extrusion force, so as to reduce the possibility of blockage of the liquid guide hole 37.

Specifically, as shown in fig. 1-2, the liquid guiding holes 37 are obliquely arranged, the included angles between the liquid guiding holes 37 and the end surfaces of the second funnel seat 35 and the second circular ring seat 36 are acute angles, and a certain included angle is formed between the oblique liquid guiding holes 37 and the vertical force applied by the extrusion seat 31, so that the possibility that fungi vertically enter the liquid guiding holes 37 is avoided, and the possibility that the liquid guiding holes 37 are blocked is reduced.

Specifically, as shown in fig. 1-2, the side wall of the tube body 1 is provided with a feed port 5, and the feed port 5 is located between the grinding seat 32 and the extrusion seat 31;

the bottom of body 1 is the circular-arc structure of undercut, and discharge gate 6 has been seted up to circular-arc structure's center department to make the appearance liquid that separates in the filter screen 4 discharge from discharge gate 6, can be convenient for like this during entering into grinding mechanism of fungus class microorganism, the discharge of appearance liquid has also been convenient for to circular-arc structure body 1 of undercut.

Because the filter screen plays the effect of filtration, can remain some residues on the filter screen during filtration, in order to reduce from the influence that the residue filters to the filter screen.

Specifically, as shown in fig. 1-2, the filter screen 4 is arranged in a funnel-shaped structure, and the filter screen 4 is fixedly connected to the bottom end of the rotating shaft rod 2, so that the residue is collected at the edge of the filter screen 4 under the centrifugal force of the filter screen 4;

be located and be provided with row cinder notch 7 on grinding seat 32 and the body 1 lateral wall directly over filter screen 4, discharge from row cinder notch 7 with the residue that will grind on seat 32 and the filter screen 4 under the centrifugal force effect of rotatory axostylus axostyle 2, the filter screen 4 of being convenient for like this is at filter screen 4 secondary edge department with the residue gathering under the effect of centrifugal force, and then discharges the residue through row cinder notch 7.

Furthermore, as shown in fig. 1-2, the pressing seat 31 is disposed adjacent to one end of the grinding seat 32, and is provided with the saw teeth 8 to enable the pressing seat 31 to catch the fungi microorganisms, so that the fungi can be tightly attached to the pressing seat 31, the fungi position can be fixed, and the fungi can be ground under the rotation action of the grinding seat 32.

A using method of a bacterial microorganism detection sample liquid extracting device comprises the following steps:

s100, putting fungi into the feed inlet, starting an external driving mechanism after the fungi are put into the feed inlet, driving the extrusion seat to be close to the grinding seat through the rotating shaft rod, crushing fungi cells under the grinding action of the grinding seat, filtering out sample liquid under the separation of the filter screen, and collecting the sample liquid through the discharge outlet.

The above embodiments are only exemplary embodiments of the present application, and are not intended to limit the present application, and the protection scope of the present application is defined by the claims. Various modifications and equivalents may be made to the disclosure by those skilled in the art within the spirit and scope of the disclosure, and such modifications and equivalents should also be considered as falling within the scope of the disclosure.

Claims

1. The utility model provides a fungus class microorganism detects appearance liquid extraction element, its characterized in that includes body (1) and installs the grinding filtering mechanism of body (1) inner chamber, grinding filtering mechanism includes:

the rotating shaft rod (2) is vertically arranged in the pipe body (1) and can rotate in a reciprocating manner under the action of external drive;

the grinding mechanism (3) is connected to the rotating shaft rod (2) along the axial direction of the rotating shaft rod (2), the grinding mechanism (3) comprises a squeezing seat (31) and a grinding seat (32), the squeezing seat (31) is in threaded connection with the rotating shaft rod (2), the grinding seat (32) is fixedly connected with the rotating shaft rod (2), the squeezing seat (31) moves back and forth along the rotating shaft rod (2) under the action of the reciprocating rotation of the rotating shaft rod (2) to be close to or far away from the grinding seat (32), and the grinding seat (32) synchronously rotates along the rotating shaft rod (2) under the action of the reciprocating rotation of the rotating shaft rod (2) to grind fungi and throw out the fungi residues through a pipeline in the pipe body (1) under the action of the centrifugal force of the grinding seat (32);

the filter screen (4) is fixedly connected with the rotating shaft lever (2) in the axial direction, and the filter screen (4) is driven by the reciprocating rotation of the rotating shaft lever (2) to synchronously rotate along with the rotating shaft lever (2) so as to filter and separate sample liquid permeating through the grinding mechanism (3).

2. The apparatus according to claim 1, wherein the sample liquid for bacterial microorganism detection is extracted from the sample liquid,

the rotating shaft rod (2) comprises a threaded section (21) and a smooth section (22), the threaded section (21) is in threaded connection with the extrusion seat (31), and the smooth section (22) is fixedly connected with the grinding seat (32) and the filter screen (4);

two guide grooves (23) are symmetrically formed in the side wall, located on two sides of the threaded section (21), of the pipe body (1), guide blocks (24) in sliding connection with the guide grooves (23) are arranged on the side wall of the extrusion seat (31), and the extrusion seat (31) moves along the guide grooves (23) to be close to or far away from the grinding seat (32) under the rotating action of the rotating shaft rod (2).

3. The apparatus according to claim 1, wherein the sample solution for measuring bacterial microorganisms is extracted from the sample solution,

the extrusion seat (31) and the grinding seat (32) are in the same shape, and the edges of the extrusion seat (31) and the grinding seat (32) are vertical to the side wall of the inner cavity of the pipe body (1);

the extrusion seat (31) comprises a first funnel seat (33) and a first circular ring seat (34), the circumferential outer side wall of the first funnel seat (33) is fixedly connected with the first circular ring seat (34), the first funnel seat (33) is in threaded connection with the threaded section (21), and a guide block (24) arranged on the first circular ring seat (34) reciprocates along the guide groove (23) under the rotation action of the rotating shaft rod (2);

the grinding seat (32) comprises a second funnel seat (35) and a second circular ring seat (36), one end of the circumferential outer side wall of the second circular ring seat (36) is fixedly connected with the second funnel seat (35), the other end of the second circular seat () is inserted into a sliding groove in the side wall of the pipe body (1), and the second funnel seat (35) is fixedly connected with the smooth section (22);

and liquid guide holes (37) are formed in the inner cavities of the second funnel seat (35) and the second circular ring seat (36), so that the grinding liquid in the grinding seat (32) flows out of the liquid guide holes (37).

4. The apparatus according to claim 1, wherein the sample liquid for bacterial microorganism detection is extracted from the sample liquid,

the liquid guide holes (37) are obliquely arranged, and included angles between the liquid guide holes (37) and the end faces of the second funnel seat (35) and the second circular ring seat (36) are acute angles.

5. The apparatus according to claim 4, wherein the sample solution for measuring bacterial microorganisms is extracted from the sample solution,

a feed inlet (5) is formed in the side wall of the pipe body (1), and the feed inlet (5) is located between the grinding seat (32) and the extrusion seat (31);

the bottom of the pipe body (1) is of a circular arc-shaped structure which is mutually sunken, and a discharge hole (6) is formed in the center of the circular arc-shaped structure, so that sample liquid separated from the filter screen (4) is discharged from the discharge hole (6).

6. The apparatus for extracting a sample liquid for use in the detection of a bacterial microorganism according to claim 2,

and a slag discharge port (7) is formed in the side wall of the pipe body (1) right above the grinding seat (32) and the filter screen (4), and residues on the grinding seat (32) and the filter screen (4) are discharged from the slag discharge port (7) under the centrifugal force action of the rotating shaft rod (2).

7. The apparatus for extracting a sample liquid for use in the detection of a bacterial microorganism according to claim 2,

filter screen (4) are hopper-shaped structure setting, and filter screen (4) rigid coupling is in the bottom of rotatory axostylus axostyle (2) to make the residue be in the gathering is in under the centrifugal force effect of filter screen (4) the edge of filter screen (4) goes out.

8. The apparatus according to claim 3, wherein the sample liquid for bacterial microorganism detection is extracted from the sample liquid,

one end of the extrusion seat (31) close to the grinding seat (32) is provided with saw teeth (8) so that the extrusion seat (31) can catch bacteria microorganisms.

9. The method of using the apparatus for extracting a sample liquid for fungus detection according to any one of claims 1 to 8, comprising the steps of: