CN115466667B - Fungus microorganism detection sample liquid extraction device and method - Google Patents
Fungus microorganism detection sample liquid extraction device and method Download PDFInfo
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- CN115466667B CN115466667B CN202211294081.1A CN202211294081A CN115466667B CN 115466667 B CN115466667 B CN 115466667B CN 202211294081 A CN202211294081 A CN 202211294081A CN 115466667 B CN115466667 B CN 115466667B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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Abstract
The invention discloses a fungus microorganism detection sample liquid extraction device, which comprises a pipe body and a grinding and filtering mechanism arranged in the inner cavity of the pipe body, wherein the grinding and filtering mechanism comprises a rotating shaft rod which is vertically arranged in the pipe body and can rotate in a reciprocating manner under the external driving effect; the grinding mechanism is connected to the rotating shaft rod along the axial direction of the rotating shaft rod, the grinding mechanism comprises an extrusion seat and a grinding seat, the extrusion seat is in spiral connection with the rotating shaft rod, the grinding seat is fixedly connected with the rotating shaft rod, the extrusion seat moves back and forth along the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod so as to be close to or far away from the grinding seat, the grinding seat rotates synchronously along the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod, the filter screen is fixedly connected along the axial direction of the rotating shaft rod, liquid is ground in a single-rotation rotary extrusion grinding mode, residues after grinding are discharged from a slag discharge port under the action of centrifugal force, discharging is convenient, and meanwhile the content of the residues in sample liquid is further reduced under the filtering of the filter screen.
Description
Technical Field
The invention relates to the technical field of extraction devices, in particular to a device and a method for extracting fungus microorganism detection sample liquid.
Background
At present, in the field of biological medicine, fungus such as tremella, ganoderma lucidum, hericium erinaceus and the like are used in a large amount as raw materials of medicines because of the medicine property, before production and preparation are carried out, fungus is usually required to be detected and analyzed, components of the fungus are usually required to be detected and crushed, fungus samples are usually required to be ground and crushed during detection, sampling liquid is extracted and analyzed in an assay mode, the samples are crushed in a knife cutting mode, and the samples are ground and extracted in a grinding mode, so that the mode is troublesome to operate.
Although the filter screen is arranged in the extraction device to filter so as to reduce the possibility of blockage of the extraction tube, residues on the grinding seat and the filter screen of the device are not easy to clean, the whole device is required to be disassembled during cleaning, the grinding seat and the filter screen are disassembled and cleaned, frequent extraction detection is not facilitated, the detection efficiency is low, and the joint is not tight due to frequent disassembly and assembly, so that the phenomenon of liquid leakage is caused.
Disclosure of Invention
The invention aims to provide a fungus microorganism detection sample liquid extraction device and a fungus microorganism detection sample liquid extraction method, so as to solve the problems in the background technology.
In order to solve the technical problems, the invention specifically provides the following technical scheme:
the utility model provides a fungus microorganism detects sample liquid extraction element, includes the body, and installs grind filtering mechanism of body inner chamber, grind filtering mechanism includes:
the rotating shaft rod is vertically arranged in the pipe body and can rotate in a reciprocating manner under the external driving effect;
the grinding mechanism is connected to the rotating shaft rod along the axial direction of the rotating shaft rod and comprises an extrusion seat and a grinding seat, the extrusion seat is in spiral connection with the rotating shaft rod, the grinding seat is fixedly connected with the rotating shaft rod, the extrusion seat moves back and forth along the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod so as to be close to or far away from the grinding seat, and the grinding seat rotates synchronously along with the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod so as to grind fungus and throw fungus residues out through a pipeline in the pipe body under the centrifugal force action of the grinding seat;
the filter screen is fixedly connected along the axial direction of the rotating shaft rod, and the filter screen synchronously rotates along with the rotating shaft rod under the reciprocating rotation action of the rotating shaft rod so as to filter and separate sample liquid permeated in the grinding mechanism.
As a preferable scheme of the invention, the rotating shaft lever comprises a thread section and a smooth section, wherein the thread section is in threaded connection with the extrusion seat, and the smooth section is fixedly connected with the grinding seat and the filter screen;
two guide grooves are symmetrically formed in the side walls of the pipe body, located on two sides of the threaded section, guide blocks are arranged on the side walls of the extrusion seat and are in sliding connection with the guide grooves, and the extrusion seat moves along the guide grooves under the rotation action of the rotating shaft rod to be close to or far away from the grinding seat.
As a preferable scheme of the invention, the shapes of the extrusion seat and the grinding seat are the same, and the edges of the extrusion seat and the grinding seat are perpendicular to the side wall of the inner cavity of the pipe body;
the extrusion seat comprises a first funnel seat and a first circular ring seat, the circumferential outer side wall of the first funnel seat is fixedly connected with the first circular ring seat, the first funnel seat is in threaded connection with the threaded section, and a guide block mounted on the first circular ring seat reciprocates along the guide groove under the rotation action of the rotating shaft rod;
the grinding seat comprises a second funnel seat and a second circular ring seat, one end of the circumferential outer side wall of the second circular ring seat is fixedly connected with the second funnel seat, the other end of the second circular ring seat is inserted into a sliding groove on the side wall of the pipe body, and the second funnel seat is fixedly connected with the smooth section;
liquid guide holes are formed in the inner cavities of the second funnel seat and the second circular ring seat, so that grinding liquid in the grinding seat flows out of the liquid guide holes.
As a preferable scheme of the invention, the liquid guide holes are obliquely arranged, and the included angles between the liquid guide holes and the end surfaces of the second funnel seat and the second circular ring seat are acute angles.
As a preferable scheme of the invention, a feed inlet is arranged on the side wall of the pipe body, and the feed inlet is positioned between the grinding seat and the extrusion seat;
the bottom of the pipe body is of an arc-shaped structure which is sunken downwards, and a discharge hole is formed in the center of the arc-shaped structure, so that sample liquid separated from the filter screen is discharged from the discharge hole.
As a preferable scheme of the invention, a slag discharge port is formed in the side wall of the pipe body right above the grinding seat and the filter screen, and residues on the grinding seat and the filter screen are discharged from the slag discharge port under the action of centrifugal force of the rotating shaft rod.
As a preferable scheme of the invention, the filter screen is arranged in a funnel-shaped structure, and the filter screen is fixedly connected to the bottom end of the rotating shaft rod, so that residues are gathered at the edge of the filter screen under the action of centrifugal force of the filter screen.
As a preferable scheme of the invention, the end of the extrusion seat close to the grinding seat is provided with saw teeth 8, so that the extrusion seat can catch fungus microorganisms.
As a preferred embodiment of the present invention, a method for using the fungus microorganism detection sample liquid extraction device comprises the steps of:
s100, placing fungus from a feed inlet, after placing, starting an external driving mechanism, driving an extrusion seat to be close to a grinding seat through a rotating shaft rod, crushing fungus cells under the grinding action of the grinding seat, filtering out sample liquid under the separation of a filter screen, and collecting through a discharge port.
And S200, after sample liquid is collected, the external driving mechanism reversely rotates, and residues on the grinding seat and the filter screen are discharged from the slag discharge port through the centrifugal force of the rotating shaft rod.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, a single-rotation rotary extrusion grinding mode is adopted for grinding liquid, and residues after grinding are discharged through the slag discharge port under the action of centrifugal force, so that the whole device is not required to be dismantled, slag discharge is convenient and rapid, continuous extraction and detection are suitable, the detection efficiency is high, and the content of residues in sample liquid is further reduced under the filtration of the filter screen.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.
FIG. 1 is a front view of the overall structure of the device of the present invention;
FIG. 2 is a cross-sectional side view of the overall structure of the device provided by the invention;
reference numerals in the drawings are respectively as follows:
1. a tube body; 2. a rotating shaft; 3. a grinding mechanism; 4. a filter screen; 5. a feed inlet; 6. a discharge port; 7. a slag discharge port; 8. saw teeth;
21. a threaded section; 22. a smooth section; 23. a guide groove; 24. a guide block;
31. extruding a base; 32. a grinding seat; 33. a first funnel base; 34. a first circular ring seat; 35. a second funnel base; 36. a second circular ring seat; 37. and a liquid guide hole.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-2, a fungus microorganism detection sample liquid extraction device comprises a tube body 1 and a grinding and filtering mechanism arranged in the inner cavity of the tube body 1, wherein the grinding and filtering mechanism comprises:
the rotating shaft lever 2 is vertically arranged in the pipe body 1 and can rotate reciprocally under the external driving effect;
the grinding mechanism 3 is connected to the rotating shaft 2 along the axial direction of the rotating shaft 2, the grinding mechanism comprises an extrusion seat 31 and a grinding seat 32, the extrusion seat 31 is in spiral connection with the rotating shaft 2, the grinding seat 32 is fixedly connected with the rotating shaft 2, the extrusion seat 31 moves back and forth along the rotating shaft 2 under the reciprocating rotation action of the rotating shaft 2 so as to be close to or far away from the grinding seat 32, and the grinding seat 32 rotates synchronously along with the rotating shaft 2 under the reciprocating rotation action of the rotating shaft 2 so as to grind fungus and throw fungus residues out through a pipeline in the pipe body 1 under the centrifugal force action of the grinding seat 32;
the filter screen 4 is fixedly connected along the axial direction of the rotating shaft rod 2, and the filter screen 4 synchronously rotates along with the rotating shaft rod 2 under the reciprocating rotation action of the rotating shaft rod 2 so as to filter and separate sample liquid permeated in the grinding mechanism 3.
The device drives the grinding mechanism and the filtering mechanism to synchronously move through the single rotating shaft rod, and the residue is discharged outside the body under the centrifugal force action of the grinding mechanism and the filtering mechanism.
Wherein, it can understand that the external driving force of rotatory axostylus axostyle can be the motor, or for the twist grip, and the preferred mode is the motor, and degree of automation is high, can improve sample liquid extraction efficiency.
Specifically, as shown in fig. 2, the rotating shaft 2 includes a threaded section 21 and a smooth section 22, the threaded section 21 is in threaded connection with the extrusion seat 31, and the smooth section 22 is fixedly connected with the grinding seat 32 and the filter screen 4;
two guide grooves 23 are symmetrically formed in the side walls of the pipe body 1, located on two sides of the threaded section 21, guide blocks 24 which are slidably connected with the guide grooves 23 are arranged on the side walls of the extrusion seat 31, and the extrusion seat 31 moves along the guide grooves 23 under the rotation action of the rotating shaft rod 2 so as to be close to or far away from the grinding seat 32.
The extrusion seat 31 is driven to rotate and lift along the thread section 21 by the rotation of the rotating shaft rod 2, and the guide block 24 on the side wall of the extrusion seat 31 is matched and connected with the guide groove 23 on the pipe body 1, so that the extrusion seat 31 only slides up and down along the rotating shaft rod 2 under the rotation action of the rotating shaft rod 2, the grinding seat 32 moves synchronously with the rotating shaft rod 2, and fungus microorganisms are ground by the grinding seat 32.
The residue is removed and polished by centrifugal force, so that residue on the polishing pad 32 is reduced, and the polished polishing liquid is gathered.
Specifically, as shown in fig. 1-2, the shapes of the extrusion seat 31 and the grinding seat 32 are the same, and the edges of the extrusion seat 31 and the grinding seat 32 are perpendicular to the side wall of the inner cavity of the pipe body 1;
the extrusion seat 31 comprises a first funnel seat 33 and a first circular ring seat 34, the circumferential outer side wall of the first funnel seat 33 is fixedly connected with the first circular ring seat 34, the first funnel seat 33 is in threaded connection with the thread section 21, and the guide block 24 arranged on the first circular ring seat 34 reciprocates along the guide groove 23 under the rotation action of the rotating shaft lever 2;
the grinding seat 32 comprises a second funnel seat 35 and a second circular ring seat 36, one end of the circumferential outer side wall of the second circular ring seat 36 is fixedly connected with the second funnel seat 35, the other end of the second circular ring seat is inserted into a chute on the side wall of the pipe body 1, and the second funnel seat 35 is fixedly connected with the smooth section 22;
the inner cavities of the second funnel seat 35 and the second circular ring seat 36 are respectively provided with a liquid guide hole 37, so that the grinding liquid in the grinding seat 32 flows out from the liquid guide holes 37.
The extruding seat 31 is matched with the grinding seat 32 for extruding, the inclined second funnel seat 35 is convenient for fungus microorganisms to slide down, the fungus microorganisms on the second funnel seat 35 are gathered on the second circular ring seat 36 under the centrifugal force of the rotating shaft lever 2, and the fungus microorganisms are ground under the extrusion of the first funnel seat 33 and the first circular ring seat 34,
the first funnel seat 33 and the first ring seat 34 always squeeze the fungus in the descending process, so that the fungus is always squeezed on the grinding seat 32, and along with the rapid rotation of the grinding seat 32, the ground juice flows out along the liquid guide hole 37.
Since the pressing seat 31 presses the grinding seat 32 vertically downwards, the force applied by the pressing seat 31 also acts vertically on the fungus, and under the action of the vertical pressing force, the residues of the fungus may be pressed into the liquid guide hole 37, so as to reduce the possibility of blocking the liquid guide hole 37.
Specifically, as shown in fig. 1-2, the liquid guiding holes 37 are obliquely arranged, the included angles between the liquid guiding holes 37 and the end surfaces of the second funnel seat 35 and the second circular ring seat 36 are acute angles, and a certain included angle is formed between the inclined liquid guiding holes 37 and the vertical force applied by the extrusion seat 31, so that the possibility that fungus vertically enters the liquid guiding holes 37 is avoided, and the possibility of blocking the liquid guiding holes 37 is reduced.
Specifically, as shown in fig. 1-2, the side wall of the pipe body 1 is provided with a feed inlet 5, and the feed inlet 5 is positioned between the grinding seat 32 and the extrusion seat 31;
the bottom of body 1 is the circular-arc structure of undercut, and discharge gate 6 has been seted up to circular-arc structure's center department to make the sample liquid that separates in the filter screen 4 discharge from discharge gate 6, in can being convenient for fungus class microorganism's entering grinding machanism like this, the circular-arc structure body 1 of undercut has also been convenient for the emission of sample liquid.
Because the filter screen plays the effect of filtration, when filtering, can remain partial residue on the filter screen, in order to reduce from the influence of residue to the filter screen filtration.
Specifically, as shown in fig. 1-2, the filter screen 4 is arranged in a funnel-shaped structure, and the filter screen 4 is fixedly connected to the bottom end of the rotating shaft lever 2, so that residues are gathered at the edge of the filter screen 4 under the action of centrifugal force of the filter screen 4;
the slag discharging port 7 is formed in the side wall of the pipe body 1 right above the grinding seat 32 and the filter screen 4, residues on the grinding seat 32 and the filter screen 4 are discharged from the slag discharging port 7 under the centrifugal force action of the rotating shaft rod 2, and therefore the filter screen 4 is convenient to gather the residues at the auxiliary edge of the filter screen 4 under the action of the centrifugal force, and the residues are discharged through the slag discharging port 7.
Further, as shown in fig. 1-2, the extrusion seat 31 is disposed proximate to one end of the grinding seat 32, and is provided with saw teeth 8 to enable the extrusion seat 31 to grip fungus microorganisms, so that the fungus can be tightly attached to the extrusion seat 31, thereby ensuring the fixation of the fungus position, and further grinding under the rotation action of the grinding seat 32.
The application method of the fungus microorganism detection sample liquid extraction device comprises the following steps:
s100, placing fungus from a feed inlet, after placing, starting an external driving mechanism, driving an extrusion seat to be close to a grinding seat through a rotating shaft rod, crushing fungus cells under the grinding action of the grinding seat, filtering out sample liquid under the separation of a filter screen, and collecting through a discharge port.
And S200, after sample liquid is collected, the external driving mechanism reversely rotates, and residues on the grinding seat and the filter screen are discharged from the slag discharge port through the centrifugal force of the rotating shaft rod.
The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application, the scope of which is defined by the claims. Various modifications and equivalent arrangements may be made to the present application by those skilled in the art, which modifications and equivalents are also considered to be within the scope of the present application.
Claims (4)
1. The utility model provides a fungus microorganism detects sample liquid extraction element, its characterized in that includes body (1) and installs grinding filter mechanism in body (1) inner chamber, grinding filter mechanism includes:
the rotating shaft rod (2) is vertically arranged in the pipe body (1) and can rotate in a reciprocating manner under the external driving effect; the rotary shaft lever (2) comprises a threaded section (21) and a smooth section (22), the threaded section (21) is in threaded connection with the extrusion seat (31), and the smooth section (22) is fixedly connected with the grinding seat (32) and the filter screen (4);
two guide grooves (23) are symmetrically formed in the side walls of the pipe body (1) located on two sides of the threaded section (21), guide blocks (24) which are connected with the guide grooves (23) in a sliding mode are arranged on the side walls of the extrusion seat (31), and the extrusion seat (31) moves along the guide grooves (23) under the rotation action of the rotating shaft rod (2) to be close to or far away from the grinding seat (32);
the grinding mechanism (3) is connected to the rotating shaft (2) along the axial direction of the rotating shaft (2), the grinding mechanism (3) comprises an extrusion seat (31) and a grinding seat (32), the extrusion seat (31) is in spiral connection with the rotating shaft (2), the grinding seat (32) is fixedly connected with the rotating shaft (2), the extrusion seat (31) moves back and forth along the rotating shaft (2) under the reciprocating rotation action of the rotating shaft (2) so as to be close to or far away from the grinding seat (32), and the grinding seat (32) rotates synchronously along with the rotating shaft (2) under the reciprocating rotation action of the rotating shaft (2) so as to grind fungus and throw fungus residues out through a pipeline in the pipe body (1) under the centrifugal force action of the grinding seat (32);
the filter screen (4) is fixedly connected along the axial direction of the rotating shaft lever (2), and the filter screen (4) synchronously rotates along with the rotating shaft lever (2) under the reciprocating rotation action of the rotating shaft lever (2) so as to filter and separate sample liquid permeated in the grinding mechanism (3); the filter screen (4) is arranged in a funnel-shaped structure, and the filter screen (4) is fixedly connected to the bottom end of the rotating shaft lever (2) so that residues gather at the edge of the filter screen (4) under the action of centrifugal force of the filter screen (4);
the shapes of the extrusion seat (31) and the grinding seat (32) are the same, and the edges of the extrusion seat (31) and the grinding seat (32) are perpendicular to the side wall of the inner cavity of the pipe body (1);
the extrusion seat (31) comprises a first funnel seat (33) and a first circular ring seat (34), the circumferential outer side wall of the first funnel seat (33) is fixedly connected with the first circular ring seat (34), the first funnel seat (33) is in threaded connection with the threaded section (21), and a guide block (24) arranged on the first circular ring seat (34) reciprocates along the guide groove (23) under the rotation action of the rotating shaft lever (2);
the grinding seat (32) comprises a second funnel seat (35) and a second circular ring seat (36), one end of the circumferential outer side wall of the second circular ring seat (36) is fixedly connected with the second funnel seat (35), the other end of the second circular ring seat (36) is inserted into a chute on the side wall of the pipe body (1), and the second funnel seat (35) is fixedly connected with the smooth section (22);
liquid guide holes (37) are formed in the inner cavities of the second funnel seat (35) and the second circular ring seat (36) so that grinding liquid in the grinding seat (32) flows out of the liquid guide holes (37);
the side wall of the pipe body (1) is provided with a feed inlet (5), and the feed inlet (5) is positioned between the grinding seat (32) and the extrusion seat (31);
the bottom of the pipe body (1) is of a circular arc structure which is sunken downwards, and a discharge hole (6) is formed in the center of the circular arc structure, so that sample liquid separated from the filter screen (4) is discharged from the discharge hole (6);
slag discharging holes (7) are formed in the side wall of the pipe body (1) right above the grinding seat (32) and the filter screen (4), and residues on the grinding seat (32) and the filter screen (4) are discharged from the slag discharging holes (7) under the action of centrifugal force of the rotating shaft lever (2).
2. The fungus microorganism detection sample liquid extraction apparatus according to claim 1, wherein,
the liquid guide holes (37) are obliquely arranged, and the included angles between the liquid guide holes (37) and the end surfaces of the second funnel seat (35) and the second circular ring seat (36) are acute angles.
3. The fungus microorganism detection sample liquid extraction apparatus according to claim 1, wherein,
the extrusion seat (31) is close to one end of the grinding seat (32) and is provided with sawteeth (8), so that the extrusion seat (31) can be used for grabbing fungus microorganisms.
4. A method of using the fungus microorganism detection sample liquid extraction apparatus according to any one of claims 1 to 3, comprising the steps of:
s100, placing fungus from a feed inlet, starting an external driving mechanism after placing, driving an extrusion seat to be close to a grinding seat through a rotating shaft rod, crushing fungus cells under the grinding action of the grinding seat, filtering out sample liquid under the separation of a filter screen, and collecting through a discharge outlet;
and S200, after sample liquid is collected, the external driving mechanism reversely rotates, and residues on the grinding seat and the filter screen are discharged from the slag discharge port through the centrifugal force of the rotating shaft rod.
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| CN202211294081.1A CN115466667B (en) | 2022-10-21 | 2022-10-21 | Fungus microorganism detection sample liquid extraction device and method |
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| CN115466667B true CN115466667B (en) | 2023-08-04 |
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| CN108624485A (en) * | 2018-05-15 | 2018-10-09 | 曹艳茹 | A kind of mushroom microorganism detection sample liquid extraction element |
| CN108680728A (en) * | 2018-05-21 | 2018-10-19 | 西南石油大学 | A kind of content measurement rammell extruding grinding mechanism |
| CN210252543U (en) * | 2019-07-11 | 2020-04-07 | 江西田友生化有限公司 | Grinding and filtering device for herbicide production |
| CN210935330U (en) * | 2019-10-28 | 2020-07-07 | 贵阳铭鼎农业科技有限公司 | Feed grinding device |
| CN211989077U (en) * | 2019-12-26 | 2020-11-24 | 太原师范学院 | Protein extraction device |
| CN211636874U (en) * | 2019-12-31 | 2020-10-09 | 洞玛生物技术(深圳)有限公司 | Plant extract extraction element |
| CN111744589A (en) * | 2020-07-02 | 2020-10-09 | 许伟益 | Chinese-medicinal material processing is with grinding extraction element |
| CN213724958U (en) * | 2020-11-24 | 2021-07-20 | 重庆化工职业学院 | Traditional chinese medicine is smashed and is drawed integrative device |
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