Fungus class microorganism detects appearance liquid extraction element
Technical Field
The invention discloses a sample liquid extraction device for detecting bacteria microorganisms, relates to a device used in bioengineering and used for extracting sample liquid when detecting the bacteria microorganisms, and belongs to the field of bioengineering. In particular to a device for extracting a bacterial microorganism sample liquid by matching an inner rotary disc and an outer rotary disc.
Background
At present, in the biological medicine field, the fungus is like the tremella, glossy ganoderma, hedgehog fungus etc. because of it has the edible property, by using in a large number as the raw and other materials of medicine, before carrying out the production preparation, generally need carry out the detection and analysis to the fungus earlier, survey its composition, generally need grind the fungus class sample when detecting and smash, extract the appearance liquid and carry out assay analysis, traditional mode is that the mode that adopts the sword to cut is broken with the sample, the appearance liquid wherein is extracted with the sample grinding through the mode of grinding, it is comparatively troublesome to adopt this kind of mode operation, when the detection volume is great, efficiency is lower.
The utility model discloses a No. CN201803870U, an ultrasonic crusher reposition of redundant personnel sampler is disclosed, including sample cup and the last sealed lid of the sealed hookup of sample cup, run through the reposition of redundant personnel sampling tube that sets up slightly on last sealed lid, it is sealed between reposition of redundant personnel sampling tube and the last sealed lid, reposition of redundant personnel sampling tube suitable for reading is provided with the flow divider, the reposition of redundant personnel sampler interface that sets up on another interface hookup ultrasonic crusher's the back flow of flow divider, when adopting the device to carry out fungus class detection sampling, sample liquid mixes together with fungus class crushing thing, make the sampling tube jam easily, need filter the crushing thing and then draw sample liquid, influence detection efficiency, excellent in use effect.
Disclosure of Invention
In order to improve the situation, the invention provides a device for extracting a bacteria microorganism detection sample liquid, which is a device for extracting a bacteria microorganism sample liquid through the matching of an inner rotary disc and an outer rotary disc. Can effectively improve the extraction efficiency of the sample liquid of the bacteria microorganism samples.
The invention relates to a device for extracting a bacteria microorganism detection sample solution, which is realized by the following steps: the invention relates to a bacteria microorganism detection sample liquid extraction device which comprises a main body device and an extraction device, wherein the main body device comprises a feeding hopper, a connecting cylinder, a discharging hopper, an end cover, a connecting sleeve, a main body cylinder and a guide sleeve, the bottom end of the main body cylinder is of an open structure, the top end of the connecting cylinder is connected with the bottom end of the main body cylinder through the connecting sleeve, the top end of the connecting cylinder is of an open structure and is communicated with the main body cylinder, the bottom end of the connecting cylinder is of an open structure, the discharging hopper is arranged at the bottom end of the connecting cylinder and is communicated with the connecting cylinder, the end cover is arranged on the discharging hopper, a guide groove is formed in the inner wall of the main body cylinder, the guide groove is spiral, a feeding hole is formed in the cylinder wall of the main body cylinder, the diameter of the feeding hole is gradually increased from an inlet to an outlet, the feeding hopper is arranged on the outer wall of the main body cylinder and is communicated with the feeding hole, the guide sleeve is arranged in the middle of the top end of the main body cylinder, the extraction device is composed of a handle, The device comprises a push rod, an outer rotating disc, a grinding plate, a filter screen, limiting blocks, rotating blocks, a fixing sleeve, a compression spring, a limiting plate, a supporting sleeve, a fixing bearing, limiting rods, an inner rotating disc, a twist rod, a sliding groove and a spiral groove, wherein the outer rotating disc is arranged in a main body cylinder, the outer side of the outer rotating disc is communicated with the inner wall of the main body cylinder, the rotating blocks are arranged on the outer side of the outer rotating disc and are positioned in a guide groove on the inner wall of the main body cylinder, the bottom of the outer rotating disc is an arc-shaped surface, the height of the outer rotating disc is gradually reduced from the middle to the edge, the bottom of the outer rotating disc is provided with the spiral groove, the outer rotating disc is provided with a placing groove, the wall of the placing groove is provided with the sliding groove, the inner rotating disc is arranged in the main body cylinder and corresponds to the placing groove, one end of each of the two limiting rods is arranged at the top of the inner rotating disc, the other end of each limiting rod is arranged in the sliding groove, each limiting rod is of an L-shaped structure, the bottom of the inner rotating disc is an arc-shaped surface, and the height of the inner rotating disc is gradually increased from the middle to the edge, the bottom of the inner rotary disk is provided with a plurality of teeth, the cross section of each tooth is of a triangular structure, the outer rotary disk is provided with a through hole and communicated with a placing groove, a supporting sleeve is arranged at the top of the outer rotary disk and corresponds to the through hole on the outer rotary disk, one end of a push rod penetrates through a guide sleeve and is arranged in the supporting sleeve, a fixed bearing is arranged between the push rod and the supporting sleeve, one end of the push rod is not contacted with the top of the outer rotary disk, a handle is arranged at the other end of the push rod, a rotating groove is arranged on the push rod, a fixed sleeve is arranged on the wall of the rotating groove, the inner wall of the fixed sleeve is provided with threads, the spiral direction is opposite to that of a guide groove on the inner wall of a main body cylinder, one end of a twist rod is arranged in the placing groove and is connected with the top of the outer rotary disk, the other end of the twist rod sequentially penetrates through the through hole on the outer rotary disk and the fixed sleeve and is arranged in the rotating groove, a limiting plate is arranged at the other end of the twist rod and is connected with the top of the rotating groove through a compression spring, the lapping plate is arranged in the connecting cylinder, the lapping plate is of a circular plate-shaped structure, the outer side and the inner wall of the connecting cylinder are attached to each other, the limiting block is arranged on the inner wall of the connecting cylinder and is in contact with the bottom of the lapping plate, the limiting block is of an annular block-shaped structure, a plurality of liquid guide holes are formed in the lapping plate, the inlet of each liquid guide hole is large and the outlet of each liquid guide hole is small, the top of the lapping plate is an arc-shaped surface, the height of the lapping plate is gradually increased from the middle to the edge, two filter screens are arranged on the inner wall of the connecting cylinder respectively, the two filter screens are distributed up and down and are respectively located below the lapping plate, and the mesh of the filter screen on the upper side is larger than the mesh of the filter screen on the lower side.
When the sample liquid is extracted for detecting bacteria microorganisms, firstly, a bacteria microorganism sample is placed into the main body cylinder through the feeding hopper and is positioned between the grinding plate and the outer rotary disc, then the handle is pressed downwards, the handle drives the push rod to move downwards, the push rod drives the outer rotary disc to move downwards, the outer rotary disc drives the rotating block to move downwards along the spiral guide groove, further the outer rotary disc moves downwards and rotates, the spiral groove at the bottom of the outer rotary disc pushes the bacteria microorganism sample to the middle part in the rotating process, at the moment, the inner rotary disc moves downwards and rotates along with the outer rotary disc, but the inner rotary disc does not rotate, when the outer rotary disc is extruded to the bacteria microorganism sample, the inner rotary disc is pushed upwards by the bacteria microorganism sample, the inner rotary disc moves towards the placing groove, the inner rotary disc drives the twist rod to move upwards, the compression spring is compressed, and the twist rod is matched with the fixing sleeve, the twist rod rotates in the upward moving process and drives the inner rotary disc to rotate, the rotating direction of the inner rotary disc is opposite to that of the outer rotary disc, the handle is pulled in a reciprocating mode, the bacteria microorganism samples in the main body cylinder are crushed through the matching of the inner rotary disc and the outer rotary disc, sample liquid generated by crushing flows into the connecting cylinder through a plurality of liquid guide holes in the grinding plate, and then enters the discharge hopper after being filtered step by step through the two filter screens;
the diameter of the feed port is gradually increased from the inlet to the outlet, so that a sample in the feed port can conveniently enter the main body cylinder, and the excessive blockage of the sample in the feed port is prevented;
the spiral groove is formed in the bottom of the outer rotary disc, so that a sample can be pushed towards the middle in the rotating process of the outer rotary disc, the inner rotary disc is convenient to crush, and the sample liquid extraction efficiency is improved;
the bottom of the outer rotary disc is an arc-shaped surface, and the height of the outer rotary disc is gradually reduced from the middle to the edge, so that a fungus microorganism sample can be extruded to the middle, and the inner rotary disc can be conveniently crushed;
the design that a plurality of teeth are arranged at the bottom of the inner rotary disc can assist the inner rotary disc to crush the bacteria microorganism samples in the rotating process of the inner rotary disc, so that the sample liquid extraction efficiency is improved;
due to the design that one end of the push rod is not in contact with the top of the outer turntable, the push rod is not affected and does not rotate when the outer turntable rotates;
the inner wall of the fixed sleeve is provided with threads, and the spiral direction of the fixed sleeve is opposite to that of the guide groove on the inner wall of the main body cylinder, so that when the inner rotary disc rotates, the rotating direction of the inner rotary disc is opposite to that of the outer rotary disc, and a sample is crushed through the matching of the inner rotary disc and the outer rotary disc;
the design that the inlet of the liquid guide hole is large and the outlet of the liquid guide hole is small is convenient for sample liquid in the main body cylinder to enter the liquid guide hole;
the top of the grinding plate is an arc-shaped surface, and the height of the grinding plate is gradually increased from the middle to the edge, so that a bacterial microorganism sample can be leaned against the middle as much as possible, and the crushing of the inner rotary disc is facilitated;
the meshes of the upper filter screen are larger than those of the lower filter screen, so that the sample liquid can be filtered step by step, and the filtering effect is improved;
the design that the bacteria microorganism samples are crushed by the matching of the handle and the outer rotary disc and the matching of the twist rod and the inner rotary disc only needs to pull the handle back and forth, so that the efficiency of extracting the sample liquid can be effectively improved;
the grinding plate is matched with the filter screen, and the design of filtering the sample liquid generated by crushing can directly filter the sample liquid generated by crushing, filter crushed samples in the sample liquid and prevent the sampling tube from being blocked;
when the inner rotary disc is contacted with a sample, the inner rotary disc starts to rotate, so that unnecessary rotation can be avoided;
the purpose of extracting the sample liquid when detecting the bacteria microorganism used in bioengineering is achieved.
Has the beneficial effects.
The structure is simple, and the device is convenient and practical.
Secondly, the price is low, and the popularization is easy.
And thirdly, the extraction efficiency of the sample liquid of the bacteria microorganism sample can be effectively improved.
Drawings
FIG. 1 is a schematic structural view of a sample liquid extraction device for detecting bacterial microorganisms according to the present invention.
FIG. 2 is a schematic structural view of a supporting sleeve of the bacteria microorganism detection sample liquid extracting device of the present invention.
FIG. 3 is a perspective view of a fixing sleeve of the device for extracting a bacterial microorganism test sample according to the present invention.
In the attached drawings
Wherein the parts are: handle (1), push rod (2), outward turning disk (3), go into hopper (4), lapping plate (5), connecting cylinder (6), go out hopper (7), end cover (8), filter screen (9), stopper (10), adapter sleeve (11), main part section of thick bamboo (12), uide bushing (13), turning block (14), fixed cover (15), compression spring (16), limiting plate (17), support cover (18), fixing bearing (19), gag lever post (20), inner dial (21), fried dough twist pole (22), sliding tray (23), helicla flute (24).
The specific implementation mode is as follows:
the invention relates to a bacteria microorganism detection sample liquid extraction device, which is realized by comprising a main body device and an extraction device, wherein the main body device comprises a feeding hopper (4), a connecting cylinder (6), a discharging hopper (7), an end cover (8), a connecting sleeve (11), a main body cylinder (12) and a guide sleeve (13), the bottom end of the main body cylinder (12) is of an open structure, the top end of the connecting cylinder (6) is connected with the bottom end of the main body cylinder (12) through the connecting sleeve (11), the top end of the connecting cylinder (6) is of an open structure and is communicated with the main body cylinder (12), the bottom end of the connecting cylinder (6) is of an open structure, the discharging hopper (7) is arranged at the bottom end of the connecting cylinder (6) and is communicated with the connecting cylinder (6), the end cover (8) is arranged on the discharging hopper (7), a guide groove is formed in the inner wall of the main body cylinder (12), the guide groove is spiral, and a feeding hole is formed in the wall of the main body cylinder (12), the diameter of the feed inlet is gradually increased from an inlet to an outlet, the feeding hopper (4) is arranged on the outer wall of the main body cylinder (12) and communicated with the feed inlet, the guide sleeve (13) is arranged in the middle of the top end of the main body cylinder (12), the extraction device is composed of a handle (1), a push rod (2), an outer rotary disc (3), a grinding plate (5), a filter screen (9), a limiting block (10), a rotating block (14), a fixing sleeve (15), a compression spring (16), a limiting plate (17), a supporting sleeve (18), a fixing bearing (19), a limiting rod (20), an inner rotary disc (21), a twist rod (22), a sliding groove (23) and a spiral groove (24), the outer rotary disc (3) is arranged in the main body cylinder (12), the outer side of the outer rotary disc (3) is communicated with the inner wall of the main body cylinder (12), the rotating block (14) is arranged on the outer side of the outer rotary disc (3) and is positioned in the guide groove on the inner wall of the main body cylinder (12), the bottom of the outer rotating disc (3) is an arc-shaped surface, the height of the outer rotating disc is gradually reduced from the middle to the edge, the bottom of the outer rotating disc (3) is provided with a spiral groove (24), the outer rotating disc (3) is provided with a placing groove, the wall of the placing groove is provided with a sliding groove (23), the inner rotating disc (21) is arranged in the main body cylinder (12) and corresponds to the placing groove, one end of each of two limiting rods (20) is respectively arranged at the top of the inner rotating disc (21), the other end of each of the two limiting rods is respectively arranged in the sliding groove (23), each of the limiting rods (20) is of an L-shaped structure, the bottom of the inner rotating disc (21) is an arc-shaped surface, the height of each of the two limiting rods is gradually increased from the middle to the edge, the bottom of the inner rotating disc (21) is provided with a plurality of teeth, the cross sections of the teeth are of a triangular structure, the outer rotating disc (3) is provided with a through hole and communicated with the placing groove, a support sleeve (18) is arranged at the top of the outer rotating disc (3) and corresponds to the through hole on the outer rotating disc (3), one end of a push rod (2) penetrates through a guide sleeve (13) and is arranged in a support sleeve (18), a fixed bearing (19) is arranged between the push rod (2) and the support sleeve (18), one end of the push rod (2) is not contacted with the top of an outer rotating disc (3), a handle (1) is arranged at the other end of the push rod (2), a rotating groove is formed in the push rod (2), a fixed sleeve (15) is arranged on the wall of the rotating groove, threads are arranged on the inner wall of the fixed sleeve (15), the spiral direction is opposite to the spiral direction of the guide groove on the inner wall of a main body cylinder (12), one end of a twist rod (22) is arranged in a placing groove and is connected with the top of the outer rotating disc (3), the other end of the twist rod (22) sequentially penetrates through a through hole in the outer rotating disc (3), the fixed sleeve (15) is arranged in the rotating groove, a limiting plate (17) is arranged at the other end of the twist rod (22) and is connected with the top of the rotating groove through a compression spring (16), grinding plate (5) are arranged in connecting cylinder (6), grinding plate (5) are circular platelike structure, and the outside laminates with connecting cylinder (6) inner wall mutually, and stopper (10) are arranged in on connecting cylinder (6) inner wall, and contact with grinding plate (5) bottom, stopper (10) are annular massive structure, it has a plurality of drain holes to open on grinding plate (5), the import in drain hole is big, the export is little, grinding plate (5) top is the arcwall face, and highly increases gradually to the edge from the centre, and on connecting cylinder (6) inner wall was arranged respectively in to two filter screen (9), two filter screen (9) distribute from top to bottom, and be located the below of grinding plate (5) respectively, filter screen (9) mesh that leans on is greater than filter screen (9) mesh that leans on down.
When the sample liquid is used for extracting bacteria microorganism, firstly, a bacteria microorganism sample is placed into the main body cylinder (12) through the feeding hopper (4) and is positioned between the grinding plate (5) and the outer rotary disc (3), then the handle (1) is pressed downwards, the handle (1) drives the push rod (2) to move downwards, the push rod (2) drives the outer rotary disc (3) to move downwards, the outer rotary disc (3) drives the rotating block (14) to move downwards along the spiral guide groove, further, the outer rotary disc (3) moves downwards and rotates, the spiral groove (24) at the bottom of the outer rotary disc (3) pushes the bacteria microorganism sample to the middle part in the rotating process, at the moment, the inner rotary disc (21) moves downwards and rotates along with the outer rotary disc (3), but the inner rotary disc (21) does not rotate, when the outer rotary disc (3) extrudes the bacteria microorganism sample, the microorganism sample in the inner rotary disc (21) is pushed upwards, the inner rotary disc (21) moves towards the placing groove, the inner rotary disc (21) drives the twist rod (22) to move upwards, the compression spring (16) is compressed, the twist rod (22) is matched with the fixing sleeve (15), the twist rod (22) moves upwards to rotate and drive the inner rotary disc (21) to rotate, the rotating direction of the inner rotary disc (21) is opposite to that of the outer rotary disc (3), the handle (1) is pulled in a reciprocating mode, bacterial microorganism samples in the main body cylinder (12) are crushed through the matching of the inner rotary disc (21) and the outer rotary disc (3), sample liquid generated by crushing flows into the connecting cylinder (6) through a plurality of liquid guide holes in the grinding plate (5), and then is filtered step by step through the two filter screens (9) and then enters the discharge hopper (7);
the diameter of the feed inlet is gradually increased from the inlet to the outlet, so that a sample in the feed inlet can conveniently enter the main body cylinder (12), and the excessive blockage of the sample in the feed inlet is prevented;
the bottom of the outer rotary disc (3) is provided with a spiral groove (24), so that a sample can be pushed towards the middle in the rotating process of the outer rotary disc (3), the inner rotary disc (21) can be conveniently crushed, and the sample liquid extraction efficiency is improved;
the bottom of the outer rotary disc (3) is an arc-shaped surface, and the height of the outer rotary disc is gradually reduced from the middle to the edge, so that a fungus microorganism sample can be extruded to the middle, and the inner rotary disc (21) can be conveniently crushed;
the bottom of the inner rotary disc (21) is provided with a plurality of teeth, so that the inner rotary disc (21) can be assisted to crush the bacteria microorganism samples in the rotating process of the inner rotary disc (21), and the sample liquid extraction efficiency is improved;
due to the design that one end of the push rod (2) is not in contact with the top of the outer turntable (3), when the outer turntable (3) rotates, the push rod (2) is not affected, and the push rod (2) does not rotate;
the inner wall of the fixed sleeve (15) is provided with threads, and the design that the spiral direction is opposite to the spiral direction of the guide groove on the inner wall of the main body cylinder (12) enables the rotating direction of the inner rotary disk (21) to be opposite to that of the outer rotary disk (3) when the inner rotary disk (21) rotates, so that a sample is crushed through the matching of the inner rotary disk (21) and the outer rotary disk (3);
the design that the inlet of the liquid guide hole is large and the outlet of the liquid guide hole is small is convenient for sample liquid in the main body cylinder (12) to enter the liquid guide hole;
the top of the grinding plate (5) is an arc-shaped surface, and the height of the grinding plate is gradually increased from the middle to the edge, so that a fungus microorganism sample can be leaned against the middle as much as possible, and the inner rotary disc (21) can be conveniently crushed;
the meshes of the upper filter screen (9) are larger than those of the lower filter screen (9), so that the sample liquid can be filtered step by step, and the filtering effect is improved;
the design that the bacteria microorganism samples are crushed through the matching of the handle (1) and the outer rotary disc (3) and the matching of the twist rod (22) and the inner rotary disc (21) is realized, and only the handle (1) needs to be pulled back and forth, so that the efficiency of extracting the sample liquid can be effectively improved;
the grinding plate (5) is matched with the filter screen (9) to filter the sample liquid generated by crushing, so that the sample liquid generated by crushing can be directly filtered, crushed samples in the sample liquid are filtered, and the sampling tube is prevented from being blocked;
when the inner rotary disc (21) is contacted with a sample, the design of self-rotation is started, and unnecessary rotation can be avoided;
the purpose of extracting the sample liquid when detecting the bacteria microorganism used in bioengineering is achieved.