CN115428661A - Application of paclobutrazol and dwarfing seedling raising method of vervain willow leaves - Google Patents

Application of paclobutrazol and dwarfing seedling raising method of vervain willow leaves Download PDF

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Publication number
CN115428661A
CN115428661A CN202211029606.9A CN202211029606A CN115428661A CN 115428661 A CN115428661 A CN 115428661A CN 202211029606 A CN202211029606 A CN 202211029606A CN 115428661 A CN115428661 A CN 115428661A
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vervain
paclobutrazol
seedling
fertilizer
dwarfing
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Inventor
詹瑞琪
陈延
杨亚新
杨晨希
刘思洋
郭锦坚
郑艺平
颜呈诚
赵俊
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Tangchao Horticulture Technology Xiamen Co ltd
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Tangchao Horticulture Technology Xiamen Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60Flowers; Ornamental plants

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses application of paclobutrazol and a method for dwarfing and growing seedlings of vervain willow leaves. After the paclobutrazol is used in the seedling stage, the stems of the vervain willow leaves are strong, the height is reduced, the lodging resistance is enhanced, a root system with better root lumps can be formed, and the flowering is not influenced in the later stage.

Description

Application of paclobutrazol and dwarfing seedling raising method of vervain willow leaves
Technical Field
The invention relates to the field of plant breeding, in particular to a seedling raising method of vervain.
Background
Verbena salicifolia (Verbena bonariensis) is a perennial herb of the genus Verbena of the family Verbenaceae, native to south America. The verbena salicifolia is a warm flower favored by long-day sunshine, the growth state of the verbena salicifolia is optimal under the full-day condition, the verbena salicifolia can bloom all the year round, the flowering period is long, and the ornamental value is high.
The verbena salicifolia is mostly applied to the flower environment as background materials or large-piece planting to build landscape effect, but the problems that the verbena salicifolia is easy to grow excessively in a plug seedling stage, leaves are easy to grow Huang Huaban and the like exist, so that seedlings are easy to fall down in the later period, and the ornamental value is poor.
The prior art is as follows:
common growth regulators mainly comprise chlormequat chloride, biejiu, ethephon and the like, and are mostly used on flowers such as chrysanthemum, poinsettia, petunia and the like. At present, vervain is usually dwarfed in the production of vervain, but the effect is not good. The paclobutrazol is used as a growth regulator for promoting plant dwarfing, compact plant type and tree crown enlargement, and has no research in plug seedling of vervain willow because the paclobutrazol is easy to cause phytotoxicity and flowering delay in production; meanwhile, at present, there is no systematic research on dwarfing cultivation management matched with vervain willow leaves.
Disclosure of Invention
The invention aims to provide a seedling raising method which enables the stems of the verbena officinalis containing willow leaves to be thick and strong, reduces the height, enhances the lodging resistance, can form a root system with better root lumps and does not influence flowering in the later period.
To achieve the above objects, the present invention provides the use of paclobutrazol as a dwarfing agent for vervain willow leaves.
Further, the paclobutrazol is used at a concentration of 10-20ppm.
The invention also provides a dwarfing seedling raising method of vervain, which is characterized in that paclobutrazol is used as a dwarfing agent, and the using concentration is 10-20ppm.
Further, paclobutrazol is used at least twice in seedling raising; preferably, the seed is used for the first time at the 14 th day of the seeding of the vervain; the second time is used when the verbena salicifolia is sowed on the 21 st day.
Further, paclobutrazol is used in the form of spray.
Further, there is also included management of,
the vervain is sown without covering vermiculite, RO water is sprayed on the substrate for 2-3 times per day during the germination period, and the illumination intensity is controlled to be 3000-5000lux at this stage;
the seedling temperature of the vervain is 31-40 ℃ in the day period, 24-28 ℃ in the night period, the humidity is 50-70%, and the illumination intensity is controlled to be 20000-30000lux; spraying fertilizer is adopted in the seedling stage, and the spraying fertilizer is applied once every 2 to 3 days: spraying the first fertilizer for 2 times, spraying the second fertilizer for 1 time, and keeping the concentration of the second fertilizer unchanged after gradually increasing the concentration from 50ppm to 150ppm; wherein the nitrogen-phosphorus-potassium content ratio of the first fertilizer is 20:10:20, containing 0.02% of trace elements, 0.12% of iron chelate, 0.01% of molybdenum, 0.015% of zinc chelate and 0.16% of sodium; the content ratio of nitrogen, phosphorus and potassium in the second fertilizer is 15:5:15, 5% of calcium, 2% of magnesium, 0.0187% of trace elements such as boron, 0.0187% of copper, 0.0187% of chelated iron, 0.075% of manganese, 0.0375%, 0.0075% of molybdenum, 0.0375% of zinc and 0.16% of sodium.
And (3) performing disease control on the vervain at the seedling later stage: spraying the mixed medicament once, wherein the mixed medicament contains equal mass of biotins, and has effects of promoting growth and inhibiting growth of bacteria, and diluting with water 1500 times before spraying.
Further, the spray fertilization adopted in the seedling stage is to use the first fertilizer for 2 times and then alternately use the second fertilizer for 1 time, the first fertilization concentration is 50ppm, the second fertilization concentration is increased to 100ppm, the third fertilization concentration is increased to 150ppm, and then the fertilization concentration in the seedling stage is kept unchanged at 150 ppm.
The dwarfing agent is used in different periods, and proper environment control and cultivation management are combined, so that the problem of late seedling lodging caused by overhigh plant height during seedling delivery can be solved; the dwarf cultivation method provided can solve the problem of leaf yellowing in the late stage of seedlings. Also solves the problems of easy phytotoxicity and delayed flowering in production, and achieves the effects of dwarfing but not delayed flowering.
Drawings
FIG. 1 is a graph showing the comparison of plant heights before shipment in the different treatments of example 1.
FIG. 2 is a diagram showing the state of seedlings before shipment in the different treatments of example 1.
Figures 3-4 are graphs comparing flowering for the clear water treatment and the paclobutrazol treatments of example 1 at different concentrations.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Flowers used in the following examples are No. 8: the content ratio of nitrogen, phosphorus and potassium is 20:10:20, wherein the TE fertilizer comprises 0.02 percent of boron, 0.12 percent of chelated iron, 0.01 percent of molybdenum, 0.015 percent of chelated zinc and 0.16 percent of sodium.
Flower number 23: the content ratio of nitrogen, phosphorus and potassium is 15:5:15, 5 percent of calcium and 2 percent of magnesium, wherein the TE fertilizer comprises 0.0187 percent of boron, 0.0187 percent of copper, 0.075 percent of chelate iron, 0.0375 percent of manganese, 0.0075 percent of molybdenum and 0.0375 percent of zinc.
The flowers No. 8 and No. 23 of the following examples may be replaced with the same or similar amounts of fertilizer.
Example 1:
the test variety is vervain, the seedling stage is from 5 months 27 days to 6 months 23 days in 2020, and 100 plants are treated in each group.
Sowing in greenhouse at 27 days 5 and 5 months in 2020, controlling illumination at 3000-5000lux during sowing, and spraying RO water for 2-3 times per day during germination period. The day temperature of the seedling stage is 31-40 ℃, the night temperature is 24-28 ℃, the humidity is 50-70%, and the illumination intensity is controlled at 20000-30000lux. The amount of the sprayed fertilizer applied flowers is 50ppm 8 at 6 th day, the amount of the sprayed fertilizer applied flowers is 100ppm 8 at the morning of 9 th 6 th day, and the amount of the sprayed fertilizer applied flowers is 150ppm 23 at 12 th day; the spray fertilization of 6 months and 15 days is more than 8 and No. 150ppm, the spray fertilization of 6 months and 18 days is more than 8 and No. 150ppm, and each plant is sprayed and fertilized for 10 seconds. Spraying paclobutrazol at different concentrations of 10, 20 and 30ppm for the first time in the evening of 6 months and 9 days (treating 100 strains respectively at different concentrations); the same treatment was applied for a second spray on day 16/6. Disease control is carried out in 20 days after 6 months, and the mixed solution is sprayed with the biotins, the shigao and the fast-curing agent (the mixed solution with the dilution multiple of each component being 1500 times). The plant height was investigated at 23 days 6 months while observing the seedling state and taking photographs. The pot was filled in 6/month and 27/month in 2020, and the full bloom period of each treatment was observed in 10/8/month.
Control experiment:
the treatment was as above except that paclobutrazol was replaced with clear water, butyrohydrazide (at concentrations 1666ppm, 3333ppm, 5000ppm, respectively), chlormequat chloride (at concentrations 333ppm, 666ppm, 1000ppm, respectively), and mepiquat chloride (at concentrations 100ppm, 150ppm, 200ppm, respectively).
In the same period, clear water, butyryl hydrazine (respectively adopting the concentrations of 1666ppm, 3333ppm and 5000 ppm), chlormequat chloride (respectively adopting the concentrations of 333ppm, 666ppm and 1000 ppm) and mepiquat chloride (respectively adopting the concentrations of 100ppm, 150ppm and 200 ppm) are adopted as controls for 3 growth regulators with different concentrations, and 100 strains of each growth regulator are treated at each concentration. And (4) processing the flower in the pot by clean water in 6-month and 27-day 2020, and observing the initial flowering phase of the clean water processing. The plant heights were measured before shipment of seedlings at 23 days 6/2020 using different concentrations of growth regulators of example 1, and the results are shown in fig. 1 and 2. Wherein FIG. 1 is a comparison graph of plant heights before shipment of different treatments. FIG. 2 is a diagram of the status of seedlings before shipment of different treatments, wherein from left to right are control, butyryl hydrazine 1666ppm, 3333ppm, 5000ppm, chlormequat chloride 333ppm, 666ppm, 1000ppm, paclobutrazol 10ppm, 20ppm, 30ppm, mepiquat chloride 100ppm, 150ppm, 200ppm.
It can be seen that the underground parts of the plants of each treatment group all grow well, and the root systems of the underground parts can be better piled. The seedling conditions of the overground part can show that the height of the clear water plant is 7cm, and the plant heights of chlormequat chloride with various concentrations, 100ppm and 200ppm are higher than that of the clear water plant. And 3333ppm and 5000ppm of butyryl hydrazine, 10ppm, 20ppm and 30ppm of paclobutrazol are used for controlling the plant height below 5.00 cm. Wherein, the internodes of the first pair and the second pair of true leaves of the plants treated by the butyryl hydrazine are both larger than 2cm, and the internodes of the first pair and the second pair of true leaves of the plants treated by the paclobutrazol in each concentration are both smaller than or equal to 2cm. And the leaves of the groups treated by 10ppm and 20ppm of paclobutrazol are dark green, and the leaves are not curled; and the paclobutrazol treatment group with 30ppm of paclobutrazol has phytotoxicity and curled leaves. In addition, compared with the leaves of plants in the control clear water, the leaves of the groups treated by paclobutrazol 10ppm and paclobutrazol 20ppm are dark green and have no yellowing phenomenon. The comprehensive performance of the hole tray seedlings treated by 10ppm and 20ppm of paclobutrazol is superior to that of clear water, butyryl hydrazine, chlormequat chloride and mepiquat chloride at various concentrations.
2020.08.10 observing that paclobutrazol treatment at 10ppm, 20ppm, 30ppm full bloom stage and clear water are substantially the same, see figures 3-4 for comparison of flowering for clear water treatment and different concentrations of paclobutrazol treatment. From right to left, the first column is the clear water control, then the paclobutrazol is 10ppm, 20ppm and 30ppm, and as can be seen from the figure, the plant treated by the clear water is the highest in height and is not dwarfed; paclobutrazol treatments at 10, 20, 30ppm had different dwarfing effects and flowering was the same as in clear water treatment. The above examples show that the use of paclobutrazol does not affect flowering. And the plants of the groups treated by the paclobutrazol are upright after the clear water falls down in 29 days after 8 months, and the plants of the groups treated by the paclobutrazol are 10ppm, 20ppm and 30 ppm.
The high-quality dwarf verbena officinalis plug seedling can be obtained by treating the verbena officinalis pithy formula with 10ppm of dwarfing agent and 20ppm of paclobutrazol at an interval of 7 days for two times in a light purple seedling stage and combining cultivation management measures such as seedling stage environment control, water fertilizer and the like, and the specific expression is that the plant stem is thick and strong, the height is reduced, the plant height is 4-5cm, and the lodging resistance is improved; the root system can form better root lumps, and the plants are easy to pull out from the plug tray after being slightly wet; and the later period does not influence the flowering.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention.

Claims (7)

1. Use of paclobutrazol as dwarfing agent for vervain willow leaf.
2. The use as claimed in claim 1, wherein paclobutrazol is used in a concentration of 10-20ppm.
3. A dwarfing seedling raising method for vervain is characterized in that paclobutrazol is used as a dwarfing agent, and the using concentration is 10-20ppm.
4. A method of dwarfing a seedling of vervain according to claim 3, characterized in that paclobutrazol is used at least twice in seedling raising; preferably, the seed is used for the first time at the 14 th day of the seeding of the vervain; the second time is used when the verbena salicifolia is sowed on the 21 st day.
5. A method for dwarfing and raising seedlings of vervain as claimed in claim 4, characterized by using paclobutrazol in the form of spray.
6. The method for dwarfing and raising seedlings of vervain, according to claim 3, characterized by comprising the following steps of management,
the vervain is sown without covering vermiculite, RO water is sprayed on the substrate for 2-3 times per day during the germination period, and the illumination intensity is controlled to be 3000-5000lux at this stage;
the seedling temperature of the vervain is 31-40 ℃ in the day period, 24-28 ℃ in the night period, the humidity is 50-70%, and the illumination intensity is controlled to be 20000-30000lux; spraying and fertilizing are adopted in the seedling stage, and spraying and fertilizing are carried out once every 2-3 days: spraying the first fertilizer for 2 times, spraying the second fertilizer for 1 time, and keeping the concentration of the second fertilizer unchanged after gradually increasing from 50ppm to 150ppm; wherein the nitrogen-phosphorus-potassium content ratio of the first fertilizer is 20:10:20, the contents of trace elements are 0.02 percent of boron, 0.12 percent of iron chelate, 0.01 percent of molybdenum, 0.015 percent of zinc chelate and 0.16 percent of sodium; the content ratio of nitrogen, phosphorus and potassium in the second fertilizer is 15:5:15, 5% of calcium, 2% of magnesium, 0.0187% of trace elements such as boron, 0.0187% of copper, 0.075% of chelated iron, 0.0375% of manganese, 0.0075% of molybdenum, 0.0375% of zinc and 0.16% of sodium;
and (3) performing disease control on the vervain at the seedling later stage: spraying the mixed medicament once, wherein the mixed medicament contains the raw bacterin with equal mass, high effect and fast effect, and is diluted by 1500 times by water before spraying.
7. A dwarf seedling method as claimed in claim 6, wherein the spray fertilization is carried out in the seedling stage by using the first fertilizer 2 times and then alternately using the second fertilizer 1 time, the first fertilization concentration is 50ppm, the second fertilization concentration is increased to 100ppm, the third fertilization concentration is increased to 150ppm, and then the fertilization concentration in the seedling stage is kept unchanged at 150 ppm.
CN202211029606.9A 2022-08-25 2022-08-25 Application of paclobutrazol and dwarfing seedling raising method of vervain willow leaves Pending CN115428661A (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
US5500404A (en) * 1993-01-28 1996-03-19 Japan Tobacco Inc. Method for dwarfing lilies and lily bulbs obtained from the dwarfing treatment
JP2005110589A (en) * 2003-10-08 2005-04-28 Ohbayashi Corp Method for growing turf grass
CN101406125A (en) * 2007-10-12 2009-04-15 会同县紫珠产业合作社 Artificial quick cultivation method of Chinese medicine plant Callicarpa kwangtungensis seedling
CN109793003A (en) * 2019-02-14 2019-05-24 江西省农业科学院土壤肥料与资源环境研究所 Sesame downgrades strong bacillum and its application technology

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5500404A (en) * 1993-01-28 1996-03-19 Japan Tobacco Inc. Method for dwarfing lilies and lily bulbs obtained from the dwarfing treatment
JP2005110589A (en) * 2003-10-08 2005-04-28 Ohbayashi Corp Method for growing turf grass
CN101406125A (en) * 2007-10-12 2009-04-15 会同县紫珠产业合作社 Artificial quick cultivation method of Chinese medicine plant Callicarpa kwangtungensis seedling
CN109793003A (en) * 2019-02-14 2019-05-24 江西省农业科学院土壤肥料与资源环境研究所 Sesame downgrades strong bacillum and its application technology

Non-Patent Citations (4)

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樊艳明: "《柳叶马鞭草栽培技术》", 《现代农村科技》, no. 16, pages 40 - 97 *
邓冬梅;李绍才;孙海龙;: "多效唑对不同立地条件下黄荆生长及生理特征的影响", 西北植物学报, no. 05 *
郭翠娥: "《柳叶马鞭草花海种植与养护》", 《中国花卉园艺》, no. 12, pages 40 - 41 *
郭翠娥;杨鹏;: "柳叶马鞭草花海种植与养护", 中国花卉园艺, no. 12, pages 40 - 41 *

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