CN115404143A - Full-automatic nucleic acid extraction appearance - Google Patents
Full-automatic nucleic acid extraction appearance Download PDFInfo
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- CN115404143A CN115404143A CN202211150177.0A CN202211150177A CN115404143A CN 115404143 A CN115404143 A CN 115404143A CN 202211150177 A CN202211150177 A CN 202211150177A CN 115404143 A CN115404143 A CN 115404143A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 69
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 69
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 69
- 238000000605 extraction Methods 0.000 title claims abstract description 61
- 230000005540 biological transmission Effects 0.000 claims abstract description 102
- 230000007246 mechanism Effects 0.000 claims abstract description 92
- 230000000712 assembly Effects 0.000 claims description 26
- 238000000429 assembly Methods 0.000 claims description 26
- 230000033001 locomotion Effects 0.000 claims description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 8
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 230000003139 buffering effect Effects 0.000 claims description 3
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 18
- 238000013461 design Methods 0.000 description 16
- 239000007788 liquid Substances 0.000 description 15
- 239000011324 bead Substances 0.000 description 10
- 230000009286 beneficial effect Effects 0.000 description 7
- 238000012423 maintenance Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 3
- 230000003044 adaptive effect Effects 0.000 description 2
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- 239000012472 biological sample Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
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- 238000005336 cracking Methods 0.000 description 1
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- 230000002195 synergetic effect Effects 0.000 description 1
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The utility model provides a full-automatic nucleic acid extraction appearance, relates to medical detection equipment technical field, solves that current equipment structure is complicated, the fault rate is high, the technique that extraction efficiency is low is not enough, and the technical scheme of adoption includes: the extraction executing mechanism is arranged on the carrying platform mechanism and comprises a top plate, a first carrier plate provided with a plurality of magnetic bar components, a second carrier plate provided with a plurality of magnetic bar sleeve components, a first driving motor driving the first carrier plate and the second carrier plate to synchronously move in a Z-axis direction through a first belt transmission mechanism, a second driving motor driving the second carrier plate to move relative to the first carrier plate through a screw rod transmission mechanism and a control module controlling the operation of the extraction instrument. According to the invention, the first support plate and the second support plate are subjected to linkage control through the first driving motor and the second driving motor, the sleeve connection or separation between the magnetic rod sleeve assembly and the magnetic rod assembly is realized through linkage control in the Z-axis direction, nucleic acid extraction is completed by matching with the carrier mechanism, and the device has the advantages of simple structure, stable operation, high efficiency and reliability.
Description
Technical Field
The invention relates to the technical field of medical detection equipment, in particular to a full-automatic nucleic acid extractor for extracting nucleic acid components in an in-vitro biological sample.
Background
With the development of life sciences, more and more biological experiments require the extraction and detection of nucleic acids (including DNA and RNA) in ex vivo biological samples to aid in the rapid diagnosis of disease. The magnetic bead method nucleic acid extraction can specifically and reversibly combine and release nucleic acid released in a sample to be extracted, so that nucleic acid separation and purification are performed. At present, the magnetic bead method for extracting nucleic acid generally comprises four main steps of cracking, combining, washing and eluting.
The basic structure of the magnetic bead method nucleic acid extractor comprises a stage mechanism for positioning and clamping the deep hole plate, and an extraction executing mechanism which is matched with the stage mechanism to finish extraction of nucleic acid in sample liquid in the deep hole plate. The extraction actuating mechanism is connected with a magnetic rod assembly matched with the deep hole plate through a first support plate, the second support plate is detachably connected with a magnetic rod sleeve assembly which is disposable and can protect the magnetic rod assembly from being in direct contact with sample liquid, the actuating driving mechanism drives the first support plate and the second support plate to move synchronously or relatively in the Z-axis direction so as to drive the magnetic rod sleeve assembly to vertically sleeve the magnetic rod or separate the magnetic rod, and the collection or release of magnetic beads in the sample liquid is realized.
At present, the execution driving mechanisms of most nucleic acid extracting instruments respectively adopt two independent sets of belt transmission mechanisms to independently drive the movement of the first carrier plate and the second carrier plate respectively, although the design can realize the cascade control of the synchronous or relative movement of the two carrier plates, the two sets of belt transmission mechanisms used by the execution driving mechanisms lead to more complex structure and higher cost, are not beneficial to the intensive and miniaturized design of the extracting instrument, have high failure rate and are inconvenient to maintain in the later period.
Secondly, the carrier mechanism of most existing nucleic acid extractors cannot move, which requires that the extraction actuator can realize X-axis movement while driving the first carrier plate and the second carrier plate to do Z-axis movement, so that the structural design of the actuator of the extraction actuator is more complicated, the volume ratio is larger, the linkage efficiency is low, and the nucleic acid extraction efficiency is affected.
Some microscope carrier mechanisms of nucleic acid extractors adopt a rotary structure design, and can drive each deep hole plate to rotate in a circumferential mode so as to correspondingly extract the magnetic rod assemblies and the magnetic rod sleeve assemblies on the actuating mechanism one by one. Or the carrier mechanisms of some nucleic acid extractors can only drive the deep hole plate to move in the X-axis direction, the action ranges of the carrier mechanisms of the two nucleic acid extractors are limited in the operation cavity of the casing, the reserved operable space between the extraction executing mechanism and the carrier mechanisms is limited, the disassembly and assembly operation of the deep hole plate is inconvenient in actual use, and the extraction efficiency is influenced during large-scale detection.
In addition, most of the existing nucleic acid extractors adopt 32-channel or 96-channel designs for extracting small-batch and large-batch nucleic acids, the positions and arrangement modes of positioning and mounting of deep-hole plates are different, and the adaptive magnetic rod sleeves are generally designed into structures such as independent 8-row, 16-row, 24-row or 96-hole magnetic rod sleeves and the like which are replaced by the whole plate. When a small amount of samples are extracted by adopting a 96-channel nucleic acid extractor, the utilization rate of the adaptive magnetic rod sleeves in 16 rows, 24 rows or 96 holes on the whole plate is low, and the magnetic rod sleeves are used as disposable consumables, so that if the magnetic rod sleeves cannot be used fully, resources are wasted, and the cost of nucleic acid extraction is increased. In addition, the magnet rod sleeves with the structures of 16 rows or 24 rows and the like are longer than the magnet rod sleeves with the structures of 8 rows in total length, so that the magnet rod sleeves are easy to be bent and deformed during insertion and use, and are difficult to assemble and disassemble.
Therefore, there is a need for improvement of the structure of the existing magnetic bead method nucleic acid extractor to overcome the above problems.
Disclosure of Invention
In conclusion, the invention aims to solve the technical defects of unreasonable structural design, complex execution driving structure, large volume ratio, low linkage efficiency, high failure rate, inconvenient assembly and disassembly of a deep hole plate, inconvenient maintenance and low extraction efficiency of the conventional full-automatic nucleic acid extractor, and provides the improved full-automatic nucleic acid extractor which has simpler and more reasonable structural design, high linkage efficiency, more convenient assembly and disassembly of the deep hole plate and a magnetic rod sleeve, lower use cost and lower maintenance cost.
In order to solve the technical defects provided by the invention, the adopted technical scheme is that the full-automatic nucleic acid extraction instrument comprises a machine shell, and is characterized in that:
the carrying platform mechanism is detachably and fixedly provided with a plurality of deep hole plates and is used for driving the deep hole plates to move in the X-axis direction and the Y-axis direction on a horizontal plane;
extract actuating mechanism, correspond to locate the top of microscope carrier mechanism, including:
the top plate is correspondingly and fixedly arranged at the upper part in the machine shell through a plurality of guide rod stand columns which are vertically arranged;
the first carrier plate is connected to the guide rod stand column in a sliding mode and is correspondingly movably positioned below the top plate, and a plurality of groups of magnetic rod assemblies which are respectively matched with the deep hole plates for use are vertically and fixedly arranged at the bottom of the first carrier plate;
the second carrier plate is connected to the guide rod stand column in a sliding mode and correspondingly movably arranged below the first carrier plate, and a plurality of groups of magnetic rod sleeve assemblies which respectively correspond to the magnetic rod assemblies and can be movably sleeved outside the magnetic rod assemblies are arranged at the bottom of the second carrier plate in a removable and detachable mode;
the first driving motor is fixedly arranged on the top plate, is in transmission connection with the first carrier plate through a first belt transmission mechanism and is used for driving the first carrier plate to drive the magnetic bar assembly to move in the Z-axis direction along the guide rod upright post;
the second driving motor is fixedly arranged on the first carrier plate, is in transmission connection with the second carrier plate through a screw rod transmission mechanism and is used for driving the second carrier plate to drive the magnetic bar sleeve assembly to move in the Z-axis direction along the guide rod upright post;
and the control module is respectively electrically connected with the second driving motor, the first driving motor and the power source of the carrier mechanism and is used for controlling the movement of the deep hole plate, the magnetic rod assembly and the magnetic rod sleeve assembly in the X-axis direction and the Z-axis direction in a linkage manner so as to finish the nucleic acid extraction action.
Further, the first belt transmission mechanism comprises:
the first driving wheel is fixedly arranged on a rotating shaft of the first driving motor;
the transmission rod is horizontally and rotatably connected to the top plate through first bearing seats arranged at two ends of the transmission rod and is parallel to a rotating shaft of the first driving motor, a first main transmission wheel is fixedly arranged at one end of the transmission rod corresponding to the first driving wheel, and a first auxiliary transmission wheel A is fixedly arranged at two ends of the transmission rod respectively;
the two fixed vertical frames are vertically and fixedly arranged at the lower parts of the two sides of the top plate correspondingly, and a first auxiliary driving wheel B correspondingly positioned below the first auxiliary driving wheel A is rotatably arranged at the lower part of each fixed vertical frame;
the first main transmission belt is horizontally sleeved on the first driving wheel and the first main transmission wheel;
the two first auxiliary transmission belts are vertically sleeved on the first auxiliary transmission wheel B and the first auxiliary transmission wheel A which are on the same side respectively;
and the two first fixing pieces are fixedly arranged on two sides of the first carrier plate respectively and are fixedly connected with the first auxiliary transmission belt on the same side in a detachable mode.
Furthermore, the second driving motor is vertically and downwardly fixedly arranged at the middle position of the upper surface of the first carrier plate, a rotating shaft of the second driving motor penetrates through the first carrier plate and extends out of one side of the second carrier plate, and a clearance hole for the second driving motor to vertically penetrate is formed in the middle position of the top plate.
Further, the screw rod transmission mechanism comprises:
the screw rod is vertically and fixedly connected to a rotating shaft of the second driving motor;
and the worm wheel is fixedly arranged at the middle position of the second carrier plate and is in threaded connection with the screw rod.
Further, the stage mechanism includes:
the bracket is movably arranged on the bottom plate of the shell through a Y-axis slide way assembly, and the front end of the bracket is provided with a handle which can be manually pushed and pulled so as to push or pull the bracket in or out;
the carrying platform is movably connected to the bracket through an X-axis slide way assembly, a plurality of clamping grooves for fixedly clamping the deep hole plates are arranged on the carrying platform, and four heating modules which are respectively and electrically connected with the control module and used for heating specified plate holes of each deep hole plate are detachably connected to each clamping groove;
and the action end of the stage driving device is connected with the stage, and the stage is driven to do X-axis motion in the horizontal plane under the control of the control module.
Furthermore, the number of the clamping grooves is 6, and the clamping grooves are in a layout of 3 rows and 2 columns; the deep hole plate on each clamping groove is provided with 96 plate holes which are arranged in 8 rows and 12 rows; bar magnet subassembly and bar magnet cover subassembly all have 6 groups and correspond the activity and be in the top of each deep hole board, the two all is 3 rows of 2 layouts and the activity corresponds from top to bottom, every group bar magnet subassembly all includes 16 and is 8 rows of 2 bar magnets of overall arrangement, every group bar magnet cover subassembly all includes 2 can cascade from head to tail assemble, 8 ally oneself with the parallel bar magnet cover of arranging.
Further, the plate holes of the 1 st, 6 th, 7 th and 12 th columns on the deep hole plate correspond to the heating modules respectively.
Furthermore, a plurality of groups of magnetic rod through hole groups which are respectively matched with the magnetic rod assemblies for vertical insertion are arranged on the body of the second carrier plate in a penetrating manner, a plurality of clamping through grooves which are respectively corresponding to the magnetic rod through hole groups in the same row are arranged at the bottom of the second carrier plate, and a drawing and inserting port positioned at the front end of the second carrier plate is arranged at the front end of each clamping through groove; the magnetic rod sleeves of the magnetic rod sleeve assembly can be spliced in a cascade mode end to end, can be sequentially positioned and assembled into the clamping through groove through the drawing and inserting port to realize cascade splicing, and are respectively aligned with the corresponding magnetic rod through hole groups.
Further, the casing is internally provided with:
the ultraviolet disinfection lamp is detachably arranged on the rear side above the carrier mechanism and is electrically connected with the control module to perform ultraviolet disinfection on the extractor after extracting nucleic acid.
Furthermore, a plurality of tension springs for buffering and stabilizing are connected between the top plate and the second carrier plate.
The invention has the beneficial effects that:
1. the extraction actuator of the invention fixes a magnetic rod assembly through a first carrier plate and fixes a magnetic rod sleeve assembly through a second carrier plate, the first carrier plate and the second carrier plate realize linkage through a second driving motor and a screw rod driving mechanism, under the control of a control module, the first carrier plate and the second carrier plate are synchronously driven by the first driving motor and the first belt driving mechanism to drive the magnetic rod assembly and the magnetic rod sleeve assembly to synchronously move in a Z-axial direction, and the second carrier plate is driven by the second driving motor and the screw rod driving mechanism to relatively move in the Z-axial direction relative to the first carrier plate so as to drive the magnetic rod sleeve assembly to be sleeved with the magnetic rod assembly or separated from the magnetic rod assembly, so that the collection or release of magnetic beads (specifically attached with nucleic acid to be extracted) in sample liquid is completed by matching with a deep hole plate in an extractor. Compared with the prior equipment which adopts two independent belt transmission mechanisms to respectively drive the first carrier plate and the second carrier plate to move, the execution driving mechanism realizes the cascade driving control of the magnetic rod assembly and the magnetic rod sleeve assembly through a simple linkage control structure, has simpler structure, more reasonable transmission layout, higher driving reliability and smaller volume ratio compared with the prior equipment, is beneficial to the integral intensive and miniaturized design of an extraction instrument, and is convenient for later maintenance, and the design of the cascade driving control structure ensures that the synergistic efficiency of the Z axial movement of the first carrier plate and the second carrier plate is higher, thereby being beneficial to improving the nucleic acid extraction efficiency.
2. According to the first belt transmission mechanism of the extraction instrument, the driving force of the first driving motor is evenly distributed to the two ends of the transmission rod by utilizing the horizontally arranged transmission rod, the first main transmission wheel, the first driving wheel and the first main transmission belt, the first carrier plate is driven to move in the Z axial direction along the guide rod stand column by utilizing the first auxiliary transmission wheels A at the two ends of the transmission rod, the first auxiliary transmission wheels B at the lower part of the fixed stand, the first auxiliary transmission belts and the first fixing pieces fixedly arranged at the two sides of the first carrier plate, so that the power distribution is more uniform, the transmission stability is better, the driving efficiency is higher, and the accuracy, the stability and the reliability of the magnetic rod assembly and the magnetic rod sleeve assembly for synchronously inserting the deep hole plate in the Z axial direction are obviously improved.
3. According to the carrier mechanism of the extraction instrument, the Y-axis movement guide of the bracket is realized through the Y-axis slide way assembly, and a user can manually pull the handle to pull the bracket and the carrier out of the machine shell together in practical use, so that the deep hole plate is convenient to disassemble and assemble, the use is more convenient, and the efficiency of large-batch nucleic acid extraction operation is obviously improved. The carrying platform is movably connected to the bracket through the X-axis slide way assembly and is driven to move in the X-axis direction through the carrying platform driving device under the control of the control module, so that the cascade drive control of the deep hole plate, the magnetic rod assembly and the magnetic rod sleeve assembly is realized by matching the first driving motor, the first belt transmission mechanism, the second driving motor and the screw rod transmission mechanism of the extraction executing mechanism, the nucleic acid extraction operation is further completed, the coordination of the cascade drive control is better, the driving efficiency is higher, and the efficiency of the nucleic acid extraction operation is obviously improved. In addition, four heating modules are detachably connected to each clamping groove of the carrying platform, so that the carrying platform can be conveniently disassembled, assembled and replaced, the maintenance and the protection are more convenient, and the sample liquid in four rows of specified plate holes on the same deep hole plate can be preheated and insulated through the four heating modules, so that the effect and the efficiency of preheating and insulating the sample liquid in the nucleic acid extraction process are improved.
4. The clamping grooves on the carrier are arranged according to a layout mode of 3 rows and 2 columns, each deep hole plate adopts a structural design of 8 rows and 12 columns of 96 holes, the layout of the magnetic rod assembly and the magnetic rod sleeve assembly is matched with the layout of the clamping grooves, a plurality of sample liquids can be simultaneously processed at one time in actual use, and the device is suitable for large-scale nucleic acid sampling detection and has higher efficiency.
5. According to the invention, the magnetic rod through hole groups on the same row are connected in series through the clamping through groove on the second carrier plate, so that the magnetic rod sleeves of an 8-row structure which can be assembled in an end-to-end cascade manner can be positioned and assembled into the clamping through groove one by one through the drawing and inserting openings and are respectively aligned with the magnetic rod through hole groups of corresponding rows, and by the design, the problems of inconvenience in assembly and disassembly, difficulty in assembly and disassembly and the like caused by the fact that the magnetic rod sleeves are integrally overlong in the using process are avoided. When the extractor is used for extracting a small amount of sample nucleic acid, a whole plate of 96-hole magnetic rod sleeves or multi-row type overlong magnetic rod sleeves are not required to be installed, corresponding nucleic acid extraction actions can be completed only through the independent 8-row magnetic rod sleeves which can be spliced in a head-to-tail cascading manner, the utilization rate of the magnetic rod sleeves is higher, resource waste is avoided, and the nucleic acid extraction cost is saved.
6. The ultraviolet disinfection lamp is arranged in the machine shell, and after the ultraviolet disinfection lamp is used, automatic disinfection and sterilization treatment in the machine shell operation cavity can be realized under the control of the control module, so that the interference of biological pollution on subsequent extraction operation is avoided.
7. A plurality of tension springs are connected between the top plate and the second carrier plate, Z-axial reaction force borne by the second carrier plate can be partially counteracted through the tension springs, the action stability of the second carrier plate is further improved, the stability and reliability of the magnetic rod sleeve assembly and the magnetic rod assembly in matching collection or release of magnetic beads in sample liquid are ensured, and the nucleic acid extraction effect of the extractor is ensured.
Drawings
FIG. 1 is a schematic view of the overall structure of the extractor of the present invention;
FIG. 2 is a schematic diagram of the internal structure of the extractor of the present invention;
FIG. 3 is a schematic diagram of an extraction actuator according to the present invention;
FIG. 4 is a rear side view of the stage mechanism of the present invention;
FIG. 5 is a schematic view of the bottom structure of the stage mechanism of the present invention;
FIG. 6 is a schematic view of an overall connection structure of the bar magnet sleeve assembly and the second carrier plate according to the present invention;
FIG. 7 is a schematic bottom structure view of a second carrier according to the present invention;
FIG. 8 is a schematic view of a connection structure of the bar magnet sleeve assembly and the card through slot according to the present invention;
FIG. 9 is a schematic view of an assembled structure of the ultraviolet disinfection lamp of the present invention;
FIG. 10 is a schematic view of the internal structure of the extractor of the present invention.
In the figure: 1. a housing, 11, a display screen, 12, an operation door, 13, a bottom plate, 14, a rear side wall, 2, a stage mechanism, 21, a bracket, 22, a stage, 221, a clamping groove, 222, a heating module, 23, a stage driving device, 231, a third driving motor, 232, a second driven wheel, 233, a second driving belt, 234, a second fixing member, 235, a fixing support, 24, Y-axis slide assembly, 25, a handle, 26, X-axis slide assembly, 3, an extraction actuator, 31, a top plate, 311, a clearance opening, 32, a first carrier plate, 321, a first sliding sleeve, 322, a magnetic rod assembly, 33, a second carrier plate, 331, a second sliding sleeve, 332, a magnetic rod sleeve assembly, 333, a magnetic rod through hole group, 334, a card through groove, 335, a drawing socket, 34, a first driving motor, 35, a second driving motor, 36, a control module, 37, a guide rod upright post, 38, a first belt transmission mechanism, 381, a first driving wheel, 382, a transmission rod, 383, a first main transmission wheel, 384, a first auxiliary transmission wheel A,385, a fixed vertical frame, 386, a first auxiliary transmission wheel B,387, a first main transmission belt, 388, a first auxiliary transmission belt, 389, a first fixed piece, 390, a first bearing seat, 39, a screw rod transmission mechanism, 391, a screw rod, 392 a worm wheel, 4, a deep hole plate, 5, an ultraviolet disinfection lamp and 6, a tension spring.
Detailed Description
The structure of the present invention will be further described with reference to the accompanying drawings and preferred embodiments of the present invention.
Referring to fig. 1 to 2, the full-automatic nucleic acid extractor of the present invention comprises a casing 1, a display screen 11 is disposed on the right side of the front end of the upper portion of the casing 1, an operation door 12 capable of sealing an operation chamber of the casing 1 is disposed on the front portion of the casing 1, a stage mechanism 2 disposed at the bottom of the operation chamber of the casing 1, and an extraction actuator 3 disposed above the stage mechanism 2 are disposed in the casing 1.
Specifically, the extraction actuator 3 includes a top plate 31, a first carrier plate 32, a second carrier plate 33, a first driving motor 34, and a second driving motor 35, and a control module 36 (as shown in fig. 9) for controlling the operation of the extraction apparatus of the present invention is further fixedly disposed at the rear side of the operation chamber in the housing 1.
Specifically, a total of 6 deep-hole plates 4 arranged in 3 rows and 2 columns are detachably fixed and arranged on the stage mechanism 2, and the stage mechanism 2 can drive the deep-hole plates 4 to move on the horizontal plane in the X-axis direction and the Y-axis direction in actual use.
Specifically, the top plate 31 is a horizontal rectangular plate-shaped structure, four guide rod vertical posts 37 are vertically and fixedly connected to the bottom plate 13 of the case 1, the guide rod vertical posts are respectively and correspondingly located at two sides of the stage mechanism 2, and the upper ends of the guide rod vertical posts are respectively and fixedly connected to four corners of the top plate 31, and the top plate 31 is correspondingly and fixedly located above the stage mechanism 2 and located at the upper portion inside the case 1 through the four guide rod vertical posts 37.
Specifically, the first carrier plate 32 is a horizontal rectangular plate-shaped structure, four corners of the first carrier plate are respectively and fixedly connected with a first sliding sleeve 321 vertically, and the first carrier plate 32 is horizontally and slidably connected to the guide rod upright 37 through the four first sliding sleeves 321 and is correspondingly and movably located below the top plate 31. 6 groups of magnetic rod assemblies 322 which are respectively matched with 6 deep hole plates 4 on the carrier platform are vertically and fixedly arranged at the bottom of the first carrier plate 32, and the magnetic rod assemblies 322 of the 6 groups are also in a 3-row 2-column layout.
Specifically, the second carrier plate 33 is a horizontal rectangular plate-shaped structure, four corners of the second carrier plate are respectively and fixedly connected with a second sliding sleeve 331 vertically, and the second carrier plate 33 is horizontally and slidably connected to the guide rod upright 37 through the four second sliding sleeves 331 and is correspondingly and movably located below the first carrier plate 32. The bottom of the second carrier 33 is provided with 6 sets of bar magnet sleeve assemblies 332 that respectively correspond to the bar magnet assemblies 322 and are movably sleeved outside the bar magnet assemblies 322, and the 6 sets of bar magnet sleeve assemblies 332 are also arranged in 3 rows and 2 rows.
Specifically, the first driving motor 34 is horizontally and transversely fixed at a position on the right rear side of the upper surface of the top plate 31, and a rotating shaft thereof is in transmission connection with the first carrier plate 32 through the first belt transmission mechanism 38, so as to drive the first carrier plate 32 to drive the magnetic rod assembly 322 to move in the Z-axis direction along the guide rod upright 37.
Specifically, the second driving motor 35 is vertically and downwardly fixedly connected to the middle position of the first carrier 32, and the rotating shaft thereof penetrates through the first carrier 32 and is in transmission connection with the second carrier 33 through the screw transmission mechanism 39, so as to drive the second carrier 33 to drive the magnetic rod sleeve assembly 332 to move in the Z-axis direction along the guide rod upright 37.
Specifically, the control module 36 is electrically connected to the display screen 11, the second driving motor 35, the first driving motor 34 and the power source of the stage mechanism 2, and is configured to control the movement of the deep hole plate 4, the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 in the X-axis direction and the Z-axis direction in a linkage manner to complete the nucleic acid extraction operation, and display information such as the operation state of the extractor through the display screen 11.
The carrier mechanism 2 of the extraction instrument can be provided with 6 deep hole plates 4 which are arranged in 3 rows and 2 columns at one time, and the layout of the magnetic rod assembly 322 on the first carrier plate 32 and the magnetic rod sleeve assembly 332 on the second carrier plate 33 is matched with the layout of the deep hole plates 4.
In an initial state, the first driving motor 34 lifts the first carrier plate 32 upwards to an upper movement limit position through the first belt transmission mechanism 38, and simultaneously the second driving motor 35 drives the second carrier plate 33 downwards to be far away from the first carrier plate 32 through the screw transmission mechanism 39, and separates the lower end of the magnetic rod assembly 322 from the second carrier plate 33, so as to conveniently disassemble and assemble the magnetic rod sleeve assembly 332, after the magnetic rod sleeve assembly 332 is completely installed, the second driving motor 35 drives the second carrier plate 33 upwards to be close to the first carrier plate 32 through the screw transmission mechanism 39, and the assembled magnetic rod sleeve assembly 332 is correspondingly inserted into the corresponding magnetic rod assembly 322.
After the deep hole plate 4 is installed, the carrier mechanism 2 drives the deep hole plate 4 to reset, and then the first driving motor 34 controls the first carrier plate 32 and the second carrier plate 33 to synchronously move downwards in the Z-axis direction through the first belt transmission mechanism 38, so as to drive the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 sleeved outside the magnetic rod assembly to correspondingly stretch into the plate hole for containing the sample liquid of the corresponding deep hole plate 4 downwards, and the magnetic bead with the specificity adsorbed nucleic acid in the sample liquid is adsorbed on the magnetic rod sleeve assembly 332 by utilizing the magnetic attraction effect. After the magnetic rod assembly 322 adsorbs magnetic beads with nucleic acids via the magnetic rod sleeve assembly 332, the control module 36 controls the first driving motor 34, the second driving motor 35 and the power source of the stage mechanism 2 to operate in an X-axis direction and a Y-axis direction in a linkage manner, so as to cooperatively control the movement of the magnetic rod assembly 322, the magnetic rod sleeve assembly 332 and the deep hole plate 4 to complete nucleic acid extraction.
After the technical scheme is adopted, the invention has the following beneficial effects.
The extraction actuator 3 of the extraction apparatus of the present invention fixes the magnetic rod assembly 322 through the first carrier 32, fixes the magnetic rod sleeve assembly 332 through the second carrier 33, the first carrier 32 and the second carrier 33 are linked through the second driving motor 35 and the lead screw driving mechanism 39, under the control of the control module 36, the first carrier 32 and the second carrier 33 are synchronously driven by the first driving motor 34 and the first belt driving mechanism 38 to drive the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 to perform Z-axial synchronous movement, and the second carrier 33 is driven by the second driving motor 35 and the lead screw driving mechanism 39 to perform Z-axial relative movement with respect to the first carrier 32 to drive the magnetic rod sleeve assembly 332 to sleeve the magnetic rod assembly 322 or separate from the magnetic rod assembly 322, so as to complete the extraction of nucleic acid in the sample liquid by cooperating with the carrier mechanism 2. The execution driving mechanism realizes the cascade driving control of the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 through the first driving motor 34, the first belt transmission mechanism 38, the second driving motor 35 and the screw rod transmission mechanism 39, and compared with the existing equipment which adopts two independent belt transmission mechanisms to respectively drive and control the first carrier plate 32 and the second carrier plate 33, the execution driving mechanism has the advantages of simpler structure, more reasonable driving layout, higher driving reliability and smaller volume ratio, is beneficial to the integral intensive and miniaturized design of an extraction instrument, and is convenient for later maintenance, and the design of the cascade driving control structure ensures that the cooperative efficiency of the axial movement of the first carrier plate 32 and the second carrier plate 33 is higher, thereby being beneficial to improving the nucleic acid extraction efficiency.
The present invention is achieved by a further improved technical means for solving the technical problems set forth above, and further includes the following technical features.
Further, referring to FIGS. 2 and 3, the first belt driving mechanism 38 of the nucleic acid isolation apparatus of the present invention includes a first driving pulley 381, a driving lever 382, a first driving pulley 383, a first auxiliary driving pulley A384, a fixing stand 385, a first auxiliary driving pulley B386, a first driving belt 387, a first auxiliary driving belt 388, and a first fixing member 389.
Specifically, the first driving wheel 381 is coaxially and fixedly arranged on a rotating shaft of the first driving motor 34, the transmission rod 382 is horizontally and transversely rotatably connected to the top plate 31 through first bearing seats 390 which are arranged at two ends of the transmission rod and respectively fixedly connected to two sides of the upper portion of the top plate 31, and the transmission rod 382 is parallel to and located at the front side of the rotating shaft of the first driving motor 34.
Specifically, the first main driving wheel 383 is coaxially and fixedly arranged at the right end of the driving rod 382 corresponding to the first driving wheel 381, and the two first auxiliary driving wheels a384 are respectively and coaxially and fixedly arranged at the left end and the right end of the driving rod 382.
Specifically, two fixed stands 385 are vertically fixed on the bottom plate 13 of the housing 1 and are respectively located at the middle positions of two sides of the stage mechanism 2, and two first auxiliary driving wheels B386 are respectively rotatably connected to the lower portions of the two fixed stands 385 and are respectively located below the first auxiliary driving wheels a384 at the same side.
Specifically, the first driving belt 387 has one belt, and is horizontally sleeved on the first driving pulley 381 and the first driving wheel 383 to transmit the driving force of the first driving motor 34 to the driving rod 382.
Specifically, two first auxiliary transmission belts 388 are respectively vertically sleeved on the first auxiliary transmission wheel B386 and the first auxiliary transmission wheel a384 which are on the same side, so as to be driven by the transmission rod 382 to rotate around the first auxiliary transmission wheel a384 and the first auxiliary transmission wheel B386 in the Z-axis direction under the driving of the first driving motor 34.
Specifically, two first fixing members 389 are respectively and fixedly disposed on two sides of the first carrier plate 32, and the two first fixing members 389 are respectively and detachably and fixedly connected to the first auxiliary transmission belt 388 on the same side.
In practical use, the rotation shaft of the first driving motor 34 drives the transmission rod 382 to horizontally and transversely rotate through the first driving wheel 381, the first driving wheel 383 and the first driving belt 387, and the first carrier plate 32 is driven to vertically move in the Z-axis direction along the guide rod upright 37 through the first secondary driving wheel a384 at two ends of the transmission rod 382, the first secondary driving belt 388 vertically sleeved on the first secondary driving wheel B386 and the first fixing member 389 in the rotation process.
The first belt transmission mechanism 38 of the extraction instrument evenly distributes the driving force of the first driving motor 34 to the two ends of the transmission rod 382 by using the horizontally arranged transmission rod 382, the first main transmission wheel 383, the first driving wheel 381 and the first main transmission belt 387, and drives the first carrier plate 32 to move in the Z-axis direction along the guide rod upright post 37 by using the first auxiliary transmission wheel a384 at the two ends of the transmission rod 382, the first auxiliary transmission wheel B386 at the lower part of the fixed upright 385, the first auxiliary transmission belt 388 and the first fixing part 389 fixedly arranged at the two sides of the first carrier plate 32, so that the power distribution is more uniform, the transmission stability is better, the driving efficiency is higher, and the accuracy, the stability and the reliability of the plate holes of the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 synchronously inserted in the Z-axis direction deep hole plate 4 are obviously improved.
In addition, the first belt transmission mechanism 38 of the present invention has a simple structure, a compact layout, a small space occupation ratio, and a smooth and efficient drive.
Preferably, the upper ends of the fixing stands 385 are respectively and fixedly connected with the side edges of the top plate 31, so that the top plate 31 is further fixed by matching with the guide rod posts 37, and the reliability and stability of the connection of the top plate 31, the first carrier plate 32 and the second carrier plate 33 with the cabinet 1 are ensured.
Further, referring to fig. 2 and 3, a second driving motor 35 of the present invention is vertically and downwardly fixed on a middle position of an upper surface of the first carrier plate 32, a rotating shaft thereof passes through the first carrier plate 32 and extends out to one side of the second carrier plate 33, and a clearance 311 for the second driving motor 35 to vertically pass through is formed on a middle position of the top plate 31.
Further, referring to fig. 3, the screw rod transmission mechanism 39 of the present invention includes a screw rod 391 and a worm wheel 392, wherein the screw rod 391 is vertically and axially fixedly connected to the rotating shaft of the second driving motor 35, and the worm wheel 392 is fixedly connected to the middle position of the second carrier plate 33 and is in threaded connection with the screw rod 391.
In practical use, the second driving motor 35 drives the screw rod 391 to rotate forward or backward, and further drives the worm wheel 392 screwed thereon to drive the second carrier plate 33 to vertically move up and down along the guide rod upright 37.
The second driving motor 35 of the present invention is disposed at the middle position of the first carrier 32, and can drive the second carrier 33 to vertically move up and down through the screw rod 391 connected to the rotating shaft thereof and the worm wheel 392 screwed on the screw rod 391, and the stress point of the second carrier 33 is at the middle position thereof, and the stability of the second carrier sliding up and down along the guide rod upright 37 can be ensured by matching with the second sliding sleeve 331 at the four corners thereof, and the present invention has the advantages of simple structure, stable operation, high driving precision and high efficiency. In addition, the clearance opening 311 on the top plate 31 can be used for the second driving motor 35 to pass through during use, so that the top plate 31 can be prevented from pressing against the second driving motor 35 when the first carrier plate 32 moves to the right position.
Further, as shown in fig. 4 to 5, stage mechanism 2 of the present invention includes a carrier 21, a stage 22, and a stage driving device 23.
Specifically, the bracket 21 is a rectangular frame structure, and is movably connected to the bottom plate 13 of the housing 1 through a Y-axis slide assembly 24 in a horizontal, front and rear direction, and a handle 25 is provided at the front end of the bracket 21 for a user to manually push and pull the bracket 21 in or out.
Specifically, the carrier 22 is a rectangular horizontal plate-shaped structure, and is movably connected to the bracket 21 through an X-axis slide assembly 26 in a horizontal left-right moving manner, 6 clamping grooves 221 which are arranged in 3 rows and 2 columns and used for fixing and clamping the deep hole plate 4 are arranged on the upper surface of the carrier 22, and four heating modules 222 which are respectively electrically connected with the control module 36 and used for heating specified plate holes of the deep hole plate 4 are detachably connected to each clamping groove 221.
Specifically, the stage driving device 23 includes a third driving motor 231, a second driving wheel (not shown in the figure), a second driven wheel 232, a second transmission belt 233 and a second fixing member 234, the third driving motor 231 is electrically connected to the control module 36, and is horizontally and longitudinally fixed to the left side of the rear end surface of the bracket 21 through a fixing support 235, the second driving wheel is coaxially and fixedly connected to the rotating shaft of the third driving motor 231 and is located in the cavity of the fixing support 235, the second driven wheel 232 is rotatably connected to the right side of the middle of the rear end surface of the bracket 21 and horizontally corresponds to the second driving wheel through the cavity of the fixing support 235, the second transmission belt 233 is horizontally and horizontally sleeved on the second driving wheel and the second driven wheel 232, and the second fixing member 234 is fixedly connected to the rear end surface of the stage 22 and detachably and fixedly connected to the second transmission belt 233.
In actual use, the control module 36 controls the rotation shaft of the third driving motor 231 to rotate, so that the second driving wheel, the second driven wheel 232 and the second transmission belt 233 drive the second fixing member 234 to drive the carrier 22 to move left and right along the X axis direction, thereby adjusting the positions of the holes in the array of the deep hole plate 4 relative to the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332.
The carrier mechanism 2 of the extractor realizes the Y-axis movement guide of the bracket 21 through the Y-axis slide assembly 24, and a user can manually pull the handle 25 to horizontally and longitudinally pull the bracket 21 and the carrier 22 out of the machine shell 1 together in practical use, so that the deep hole plate 4 is convenient to disassemble and assemble, the use is more convenient, and the efficiency of large-batch nucleic acid extraction operation is obviously improved. The carrier 22 is movably connected to the bracket 21 through the X-axis slide assembly 26, and is driven to move in the X-axis direction by the carrier driving device 23 under the control of the control module 36, so that the cascade drive control of the deep hole plate 4, the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 is realized by the first driving motor 34, the first belt transmission mechanism 38, the second driving motor 35 and the lead screw 391 transmission mechanism 39 of the extraction executing mechanism 3, and the nucleic acid extraction operation is further completed. In addition, four heating modules 222 are detachably connected to each clamping groove 221 of the carrying platform 22, the heating modules 222 can be conveniently disassembled, assembled and replaced, maintenance and preservation are more convenient, and the sample liquid in the specified plate holes on the same deep hole plate 4 can be preheated and preserved through the four heating modules 222, so that the effect and efficiency of sample liquid preheating and preserving treatment in the nucleic acid extraction process are improved.
Further, referring to fig. 2 to 9, there are 6 clamping grooves 221 on the carrier 22 of the present invention, which are arranged in 3 rows and 2 columns, and the deep hole plate 4 on each clamping groove 221 is provided with 96 plate holes arranged in 8 rows and 12 columns. In cooperation, each of the magnetic rod assemblies 322 and the magnetic rod sleeve assemblies 332 of the present invention is provided with 6 sets and is correspondingly movably positioned above each deep hole plate 4, each of the magnetic rod assemblies 322 and the magnetic rod sleeve assemblies 332 are in 3 rows and 2 rows and are movably corresponding up and down, each of the magnetic rod assemblies 322 includes 16 magnetic rods which are in 8 rows and 2 rows and are matched with the deep hole plate 4 with 96-hole structural design, and each of the magnetic rod sleeve assemblies 332 of the present invention includes 2 parallel magnetic rod sleeves which are assembled in a head-to-tail cascade manner and are in 8 rows.
Further, the plate holes of the 1 st, 6 th, 7 th and 12 th rows in the deep hole plate 4 according to the present invention correspond to the heating modules 222, respectively.
The clamping grooves 221 on the carrier 22 are arranged according to a layout mode of 3 rows and 2 columns, each deep hole plate 4 adopts a structural design of 8 rows and 12 columns of 96 holes, the layout of the magnetic rod assembly 322 and the magnetic rod sleeve assembly 332 is matched with the layout of the clamping grooves 221, a plurality of sample liquids can be simultaneously processed at one time in actual use, and the device is suitable for large-scale nucleic acid sampling detection and has higher nucleic acid extraction efficiency.
Further, as shown in fig. 6 to 8, the body of the second carrier plate 33 of the present invention is penetratingly provided with 6 groups of magnetic rod through hole groups 333 respectively matching with the magnetic rod assemblies 322 for vertical insertion, the magnetic rod through hole groups 333 are also arranged in 3 rows and 2 rows, each group of magnetic rod through hole groups 333 includes two parallel 8-hole magnetic rod through holes respectively matching with 16 magnetic rods on the corresponding magnetic rod assemblies 322. The bottom of the second carrier 33 is longitudinally provided with 4 clamping through grooves 334 corresponding to the through hole groups 333 of the magnetic rods in the same row, and each of the front ends of the clamping through grooves 334 is provided with a drawing socket 335 at the front end of the second carrier 33.
Specifically, the magnetic rod sleeves of the magnetic rod sleeve assemblies 332 can be assembled in a cascade manner end to end, and can be positioned and assembled into the clamping through groove 334 sequentially through the drawing and inserting port 335 to realize the cascade assembly and respectively align with the corresponding magnetic rod through hole group 333.
In actual use, an 8-row magnetic rod sleeve can be pushed into the front section of the card through groove 334 from the extraction and insertion opening 335 to be aligned with the magnetic rod through hole group 333 in the first row; the second 8-row magnetic rod sleeve can be pushed into the same clamping through groove 334 through the drawing and inserting port 335, the previous magnetic rod sleeve is pushed to enable the two to be spliced into a whole in an end-to-end cascade manner, and the previous magnetic rod sleeve is pushed to enable the previous magnetic rod sleeve to move backwards along the clamping through groove 334 until the previous magnetic rod sleeve is aligned with the magnetic rod through hole group 333 of the second row; the third 8-row magnetic rod sleeve can be pushed into the same clamping through groove 334 through the drawing and inserting port 335, the first two magnetic rod sleeves are pushed to enable the three to be spliced into a whole in an end-to-end cascading mode, the first two magnetic rod sleeves are pushed to enable the two magnetic rod sleeves to continuously move backwards along the clamping through groove 334 until the magnetic rod sleeve inserted last is aligned with the magnetic rod through hole group 333 of the first row, and therefore the magnetic rod sleeve assembling is completed.
After the magnetic rod sleeve is used, three magnetic rod sleeves which are assembled in a cascade mode can be pulled out of the clamping through groove 334 together through the drawing-out socket 335 at one time, so that the magnetic rod sleeves are more convenient and labor-saving to disassemble.
According to the invention, the plurality of magnetic rod through hole groups 333 in the same row are connected in series on the second carrier plate 33 through the clamping through groove 334, so that the magnetic rod sleeves of an 8-row structure which can be assembled in an end-to-end cascade manner can be positioned and assembled into the clamping through groove 334 one by one through the drawing-inserting holes 335 and are respectively aligned with the magnetic rod through hole groups 333 with corresponding row number, and the design avoids the problem that the magnetic rod sleeves are designed into an integrated whole plate 96-hole magnetic rod sleeve or a multi-row 16-row or 24-row overlong structure, and the like, and avoids the problems of inconvenient assembly and disassembly, difficult assembly and disassembly and the like caused by the overlong whole multi-row magnetic rod sleeve in the use process. When the extractor is used for extracting a small amount of sample nucleic acid, the corresponding nucleic acid extraction action can be completed only through the independent 8-row magnetic rod sleeves which can be spliced in an end-to-end cascade manner without installing overlong magnetic rod sleeves, and the whole plate with 96 holes or multiple rows of magnetic rod sleeves does not need to be assembled.
Further, referring to fig. 9, an ultraviolet disinfection lamp 5 is further disposed in the cabinet 1 of the present invention.
Specifically, the ultraviolet disinfection lamp 5 is detachably horizontally and transversely fixed on the rear side wall 14 of the operation chamber of the machine case 1 and is located on the rear side above the stage mechanism 2, and the ultraviolet disinfection lamp 5 is electrically connected with the control module 36 to perform ultraviolet disinfection and killing on the inside of the operation chamber of the extraction instrument after nucleic acid is extracted.
The ultraviolet disinfection lamp 5 is arranged in the machine shell 1, after the machine shell is used, the automatic disinfection and killing treatment in the operation cavity of the machine shell 1 can be realized under the control of the control module 36, and the interference of biological pollution to the subsequent extraction operation is avoided. In addition, the ultraviolet disinfection lamp 5 is fixedly connected to the rear side wall 14 of the operation chamber of the machine shell 1 in a detachable mode, so that the disassembly, the assembly and the replacement are convenient, and the later maintenance is convenient.
Further, referring to fig. 10, four tension springs 6 for buffering and stabilizing are elastically connected between the top plate 31 and two sides of the second carrier plate 33.
In the invention, the Z-axis reaction force applied to the second carrier plate 33 can be partially offset between the top plate 31 and the second carrier plate 33 through the additionally arranged tension spring 6, so that the action stability of the second carrier plate 33 is further improved, the stability and reliability of the magnetic rod sleeve assembly 332 and the magnetic rod assembly 322 for collecting or releasing magnetic beads in sample liquid in a matching manner are ensured, and the nucleic acid extraction effect of the extractor is ensured.
The above examples are only for the purpose of clarifying the technical solution of the present invention, and are not intended to limit the embodiments of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention, and it is intended that all such modifications and alterations be considered as within the scope of this invention.
Claims (10)
1. The utility model provides a full-automatic nucleic acid extraction appearance, includes the casing, its characterized in that, be equipped with in the casing:
the carrying platform mechanism is detachably and fixedly provided with a plurality of deep hole plates and is used for driving the deep hole plates to move in the X-axis direction and the Y-axis direction on a horizontal plane;
extract actuating mechanism, correspond to locate the top of microscope carrier mechanism, including:
the top plate is correspondingly and fixedly arranged at the upper part in the machine shell through a plurality of guide rod stand columns which are vertically arranged;
the first carrier plate is connected to the guide rod stand column in a sliding mode and is correspondingly movably positioned below the top plate, and a plurality of groups of magnetic rod assemblies which are respectively matched with the deep hole plates for use are vertically and fixedly arranged at the bottom of the first carrier plate;
the second carrier plate is connected to the guide rod stand column in a sliding mode and correspondingly movably arranged below the first carrier plate, and a plurality of groups of magnetic rod sleeve assemblies which respectively correspond to the magnetic rod assemblies and can be movably sleeved outside the magnetic rod assemblies are arranged at the bottom of the second carrier plate in a removable and detachable mode;
the first driving motor is fixedly arranged on the top plate, is in transmission connection with the first carrier plate through a first belt transmission mechanism and is used for driving the first carrier plate to drive the magnetic bar assembly to move in the Z-axis direction along the guide rod upright post;
the second driving motor is fixedly arranged on the first carrier plate, is in transmission connection with the second carrier plate through a screw rod transmission mechanism and is used for driving the second carrier plate to drive the magnetic rod sleeve assembly to move in the Z-axis direction along the guide rod upright post;
and the control module is respectively electrically connected with the second driving motor, the first driving motor and the power source of the carrier mechanism and is used for controlling the movement of the deep hole plate, the magnetic rod assembly and the magnetic rod sleeve assembly in the X-axis direction and the Z-axis direction in a linkage manner so as to finish the nucleic acid extraction action.
2. The automatic nucleic acid extractor of claim 1, wherein the first belt transmission mechanism comprises:
the first driving wheel is fixedly arranged on a rotating shaft of the first driving motor;
the transmission rod is horizontally and rotatably connected to the top plate through first bearing seats arranged at two ends of the transmission rod and is parallel to a rotating shaft of the first driving motor, a first main transmission wheel is fixedly arranged at one end of the transmission rod corresponding to the first driving wheel, and a first auxiliary transmission wheel A is fixedly arranged at two ends of the transmission rod respectively;
the two fixed vertical frames are vertically and fixedly arranged at the lower parts of the two sides of the top plate correspondingly, and a first auxiliary driving wheel B correspondingly positioned below the first auxiliary driving wheel A is rotatably arranged at the lower part of each fixed vertical frame;
the first main transmission belt is horizontally sleeved on the first driving wheel and the first main transmission wheel;
the two first auxiliary transmission belts are vertically sleeved on the first auxiliary transmission wheel B and the first auxiliary transmission wheel A which are on the same side respectively;
and the two first fixing pieces are fixedly arranged on two sides of the first carrier plate respectively and are fixedly connected with the first auxiliary transmission belt on the same side in a detachable mode.
3. The automatic nucleic acid extractor of claim 1, wherein the second driving motor is vertically and downwardly fixed to a middle position of the upper surface of the first carrier, the rotating shaft of the second driving motor extends out to one side of the second carrier through the first carrier, and a clearance for the second driving motor to vertically pass through is formed in a middle position of the top plate.
4. The automatic nucleic acid extractor of claim 3, wherein the screw driving mechanism comprises:
the screw rod is vertically and fixedly connected to a rotating shaft of the second driving motor;
and the worm gear is fixedly arranged at the middle position of the second carrier plate and is in threaded connection with the screw rod.
5. The automatic nucleic acid extractor of claim 1, wherein the stage mechanism comprises:
the bracket is movably arranged on the bottom plate of the shell through a Y-axis slide way assembly, and the front end of the bracket is provided with a handle which can be manually pushed and pulled so as to push or pull the bracket in or out;
the carrying platform is movably connected to the bracket through an X-axis slideway component, a plurality of clamping grooves for fixedly clamping the deep hole plates are arranged on the carrying platform, and each clamping groove is detachably connected with four heating modules which are respectively and electrically connected with the control module and used for heating specified plate holes of each deep hole plate;
and the actuating end of the stage driving device is connected with the stage, and the stage is driven to do X-axis movement in the horizontal plane under the control of the control module.
6. The automatic nucleic acid extracting instrument according to claim 5, wherein the number of the clamping grooves is 6, and the arrangement is 3 rows and 2 columns; the deep hole plate on each clamping groove is provided with 96 plate holes which are distributed in 8 rows and 12 rows; bar magnet subassembly and bar magnet cover subassembly all have 6 groups and correspond the activity and be in the top of each deep hole board, the two all is 3 rows of 2 row overall arrangements and the activity corresponds from top to bottom, every group bar magnet subassembly all includes 16 and is the bar magnet of 8 rows of 2 row overall arrangements, every group bar magnet cover subassembly all includes 2 can cascade assembly, 8 parallel bar magnet cover of arranging of linking up.
7. The automatic nucleic acid analyzer of claim 6, wherein the wells of the 1 st, 6 th, 7 th and 12 th rows on the deep-well plate correspond to the heating module, respectively.
8. The automatic nucleic acid extractor of claim 1, wherein the second carrier has a plurality of magnetic rod through holes penetrating through the body, the magnetic rod through holes are respectively matched with the magnetic rod assembly for vertical insertion, the bottom of the second carrier has a plurality of clamping through grooves corresponding to the magnetic rod through holes in the same row, and the front end of each clamping through groove has a plug-in hole at the front end of the second carrier; the magnetic rod sleeves of the magnetic rod sleeve assembly can be spliced in a cascade mode end to end, can be sequentially positioned and assembled into the clamping through groove through the drawing and inserting port to realize cascade splicing, and are respectively aligned with the corresponding magnetic rod through hole groups.
9. The automatic nucleic acid extractor of claim 1, wherein said housing further comprises:
the ultraviolet disinfection lamp is detachably arranged on the rear side above the carrier mechanism and is electrically connected with the control module to perform ultraviolet disinfection on the extractor after extracting nucleic acid.
10. The automatic nucleic acid extractor of claim 1, wherein a plurality of tension springs for buffering and stabilizing are connected between the top plate and the second carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211150177.0A CN115404143A (en) | 2022-09-21 | 2022-09-21 | Full-automatic nucleic acid extraction appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211150177.0A CN115404143A (en) | 2022-09-21 | 2022-09-21 | Full-automatic nucleic acid extraction appearance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115404143A true CN115404143A (en) | 2022-11-29 |
Family
ID=84166799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211150177.0A Pending CN115404143A (en) | 2022-09-21 | 2022-09-21 | Full-automatic nucleic acid extraction appearance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115404143A (en) |
-
2022
- 2022-09-21 CN CN202211150177.0A patent/CN115404143A/en active Pending
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