CN218345417U

CN218345417U - A counterpoint sampling mechanism for among nucleic acid extraction appearance

Info

Publication number: CN218345417U
Application number: CN202222498726.5U
Authority: CN
Inventors: 张吉涛; 王显付; 张超铝; 王雄
Original assignee: Shenzhen Huisong Technology Development Co ltd
Current assignee: Shenzhen Huisong Technology Development Co ltd
Priority date: 2022-09-21
Filing date: 2022-09-21
Publication date: 2023-01-20
Anticipated expiration: 2032-09-21

Abstract

The utility model provides a counterpoint sampling mechanism for in nucleic acid extraction appearance, relates to medical detection equipment technical field, solves that current equipment structural design is unreasonable, the sample is handled the less, deep hole board and bar magnet cover subassembly dismouting inconvenient, the technical insufficiency of inefficiency, and the technical scheme of adoption includes: the microscope carrier mechanism can move horizontally along the X axial direction and the Y axial direction, 6 deep hole plates which are in 3 rows and 2 columns and are 96 holes are detachably clamped on the microscope carrier mechanism, the extraction executing mechanism comprises a first carrier plate, a second carrier plate and a carrier plate driving mechanism which drives the first carrier plate and the second carrier plate to synchronously or relatively move in the Z axial direction, 6 groups of magnetic rod assemblies which are in 3 rows and 2 columns are arranged on the first carrier plate, and 6 groups of magnetic rod sleeve assemblies can be detachably arranged on the second carrier plate in a pulling and inserting mode. The utility model discloses can once only handle the sample liquid in the 6 deep hole boards, usable microscope carrier mechanism drives each deep hole board and exposes so that it goes out for drawing actuating mechanism is advanced in Y axle direction simultaneously, is convenient for change deep hole board and bar magnet cover, easy operation, convenience, high efficiency.

Description

A counterpoint sampling mechanism for among nucleic acid extraction appearance

Technical Field

The invention relates to the technical field of medical detection equipment, in particular to a para-position sampling mechanism used in a nucleic acid extractor.

Background

With the development of life sciences, more and more biological experiments require the extraction and detection of nucleic acids (including DNA and RNA) from ex vivo biological samples to aid in the rapid diagnosis of diseases. The magnetic bead method nucleic acid extraction can specifically and reversibly combine and release nucleic acid released in a sample to be extracted, so that nucleic acid separation and purification are performed. At present, the magnetic bead method for extracting nucleic acid generally comprises four main steps of cracking, combining, washing and eluting.

The main mechanism of the magnetic bead method nucleic acid extractor is a contraposition sampling mechanism which is used for matching and contraposition of a magnetic rod assembly, a magnetic rod sleeve assembly and a deep hole plate and carrying out linkage, and comprises a platform mechanism which is used for positioning and clamping the deep hole plate and driving the deep hole plate to move horizontally, and an extraction executing mechanism which is used for linkage control of the magnetic rod sleeve assembly and the magnetic rod assembly in the Z-axis direction to move and complete extraction of nucleic acid in a sample liquid of the deep hole plate by matching with the platform mechanism.

Nucleic acid extractors typically employ 32-channel or 96-channel designs for small sample extraction or large sample extraction, respectively. At present, most of 96-channel nucleic acid extractors are not reasonable in structural design of a para-position sampling mechanism, and usually adopt a structural design of a rotary carrier or a fixed carrier, so that although the number of deep-hole plates for single operation can be increased to a certain extent, and the sample amount for single extraction is increased, the sample processing capacity and efficiency are limited, and the instrument is not suitable for being used in large-scale nucleic acid sampling detection.

Secondly, its action scope of microscope carrier mechanism of the instrument is limited to inside the operation cavity of extraction appearance casing for most existing nucleic acid extraction appearance, thereby can only drive deep hole board X axial displacement or pivoting and draw bar magnet cover subassembly and bar magnet subassembly counterpoint on the actuating mechanism, and draw the spacing between actuating mechanism and microscope carrier mechanism again limited, the dismouting that leads to deep hole board, bar magnet cover subassembly when using is comparatively inconvenient, has influenced and has drawed operating efficiency, is not suitable for using in large-scale nucleic acid sampling detection.

In addition, the existing nucleic acid extraction instruments mostly adopt 32-channel or 96-channel designs for small-batch and large-batch nucleic acid extraction respectively, the positions and the arrangement modes of the positioning and the installation of the deep-hole plates are different, and the adaptive magnetic rod sleeves are generally designed into independent 8-row, 16-row or 24-row structures. If a nucleic acid extractor with 96 channels is adopted to extract a small amount of samples, the utilization rate of the adaptive magnetic rod sleeves in 16 or 24 rows is low, and the magnetic rod sleeves are used as disposable consumables, so that if the magnetic rod sleeves cannot be used fully, resources are wasted, and the cost of nucleic acid extraction is increased. In addition, the magnet rod sleeves with the structures of 16 rows or 24 rows and the like are longer than the magnet rod sleeves with the structures of 8 rows in total length, so that the magnet rod sleeves are easy to be bent and deformed during insertion and use, and are difficult to assemble and disassemble.

Therefore, it is necessary to improve the structure of the para-position sampling mechanism of the existing nucleic acid extractor to overcome the above problems.

Disclosure of Invention

In summary, the present invention aims to solve the technical deficiencies of the existing alignment sampling mechanism for nucleic acid extraction apparatus, such as unreasonable structural design, small number of processed samples, low efficiency, inconvenient assembly and disassembly of the deep-well plate and magnetic rod sleeve assembly, and low efficiency of nucleic acid extraction, and provide an alignment sampling mechanism for nucleic acid extraction apparatus, which can simultaneously extract nucleic acids from 6 deep-well plate sample solutions in a single operation, and is more convenient to assemble and disassemble the deep-well plate and magnetic rod sleeve assembly.

In order to solve the technical defects provided by the invention, the adopted technical scheme is that the para-position sampling mechanism for the nucleic acid extractor is characterized by comprising the following components:

the carrying platform mechanism can move on a horizontal plane along the X axial direction and the Y axial direction, 6 clamping grooves which are in 3-row and 2-row layout are arranged on the carrying platform mechanism in a matrix manner, a deep hole plate is detachably clamped on each clamping groove, and plate holes which are in 96-hole and 8-row and 12-row layout are arranged on each deep hole plate in a matrix manner;

draw actuating mechanism, locate microscope carrier mechanism's top, including:

the first carrier plate is correspondingly and movably arranged above each deep hole plate through a bracket component, and 6 groups of magnetic rod components which are respectively matched with each deep hole plate and are arranged in a matrix manner and are in 3 rows and 2 columns;

the second carrier plate is movably connected to the support assembly and correspondingly movably arranged below the first carrier plate, and 6 groups of magnetic rod sleeve assemblies which respectively correspond to the magnetic rod assemblies and can be movably sleeved outside the magnetic rod assemblies are arranged on the second carrier plate in a pluggable and detachable manner;

and the support plate driving mechanism is in linkage connection with the second support plate and the first support plate and is used for driving the second support plate and the first support plate to synchronously or relatively move up and down in the Z-axis direction so as to control the magnetic bar sleeve assembly to be sleeved with or separated from the magnetic bar assembly.

Furthermore, each group of magnetic rod assemblies comprises 16 magnetic rods which are arranged in 8 rows and 2 rows, and the distance between the two rows of magnetic rods of each group of magnetic rod assemblies is matched with the total width of 4 rows of plate holes on the deep hole plate; each group of magnetic rod sleeve components comprises 2 parallel magnetic rod sleeves which can be spliced in a cascade mode end to end and are arranged in 8 rows.

Furthermore, 6 groups of magnetic rod through hole groups which are arranged in 3 rows and 2 rows and are respectively matched with the magnetic rod assemblies for vertical penetration of the magnetic rods are arranged on the body of the second carrier plate in a penetrating manner, and each group of magnetic rod through hole groups comprises 8 parallel through holes; the bottom of the second carrier plate is provided with 4 clamping through grooves corresponding to the 8-row through holes in the same row respectively, and the front end of each clamping through groove is provided with a drawing and inserting hole positioned at the front end of the second carrier plate; the magnetic rod sleeves can be sequentially inserted into the same card through groove through the drawing and inserting port, positioned to the corresponding through holes of the 8-row connector and realize cascade connection, and can be drawn out through the drawing and inserting port at one time.

Furthermore, every the joint lead to all be equipped with three groups and correspond respectively and be in the same three of arranging the elasticity of 8 antithetical couplets within a definite range of via hole section and push up the pearl structure, every the bar magnet sheathe in all be equipped with can with elasticity pushes up the positioning channel groove that pearl structure cooperation joint or break away from.

Further, the magnetic rod sleeve comprises:

the base plate is of a horizontally and longitudinally extending plate-shaped structure, and 8 sleeves with upper openings on the body are vertically arranged on the base plate at equal intervals;

the cascade ring is of a closed ring structure and is fixedly arranged at the front end of the substrate;

and the three-end buckle is a clamping groove combined buckle structure with an opening at the rear end, is fixedly arranged at the tail end of the substrate and can be matched and clamped with the cascade ring.

Furthermore, each clamping groove is detachably connected with four heating modules which are respectively positioned below the plate holes in the 1 st row, the 6 th row, the 7 th row and the 12 th row on the deep hole plate and used for heating the specified plate holes.

Further, the stage mechanism includes:

the bracket is movably arranged on the bottom plate of the extraction instrument through a Y-axis slideway component, and the front end of the bracket is provided with a handle which can be manually pushed and pulled to push the bracket in or pull the bracket out;

the carrying platform is movably connected to the bracket through an X-axis slide way assembly, and the clamping grooves are arranged on the upper surface of the carrying platform in a matrix manner;

and the platform driving motor is fixedly arranged on the bracket, is in transmission connection with the platform through a belt transmission assembly and is used for driving the platform to move left and right in the X-axis direction.

Furthermore, the bracket is provided with a stable wheel which can be rotatably pressed on the bottom plate of the extraction instrument and is used for stabilizing the movement of the bracket in the Y-axis direction.

The beneficial effects of the invention are as follows:

1. the platform mechanism can move along the X axis direction and the Y axis direction on a horizontal plane, and 6 deep-hole plates arranged on the platform mechanism can be driven by the platform mechanism to move back and forth along the Y axis during actual use, so that each deep-hole plate can be exposed relative to each magnetic rod sleeve component and each magnetic rod component of the extraction executing mechanism, the disassembly and assembly operation of each deep-hole plate and each magnetic rod sleeve is convenient, and the plate changing operation of the deep-hole plate and the magnetic rod sleeve replacement efficiency during large-scale nucleic acid sampling detection are obviously improved. Moreover, the clamping grooves on the carrier mechanism are clamped with 6 deep-hole plates according to a layout mode of 3 rows and 2 columns, each deep-hole plate adopts a structural design of 8 rows and 12 columns of 96 holes, the layouts of the magnetic rod assemblies and the magnetic rod sleeve assemblies are matched with the layout of the clamping grooves, and sample liquid in the 6 deep-hole plates can be simultaneously processed at one time in actual use, so that the sample processing quantity in single operation is obviously improved, the utilization rate of the nucleic acid extractor in unit time is favorably improved, the processing efficiency is improved, and the device is suitable for being used in large-scale nucleic acid sampling detection to improve the extraction efficiency. In addition, the carrying platform mechanism can be matched with the magnetic rod sleeve assembly and the magnetic rod assembly to move left and right along the X axis, so that the carrying platform mechanism is matched with the carrying plate driving mechanism to realize accurate and efficient alignment extraction operation between the magnetic rod and 12 rows of plate holes of each deep hole plate, the linkage efficiency of extraction execution actions is high, the alignment sampling accuracy is high, and the efficiency of nucleic acid extraction is further improved.

2. According to the invention, the 8-row through holes in the same row are connected in series through the 4 clamping through grooves on the second carrier plate, so that the magnetic rod sleeves of the 8-row structure which can be assembled in an end-to-end cascading manner can be positioned and assembled into the clamping through grooves one by one through the drawing and inserting ports and are respectively aligned with the 8-row through holes of the corresponding row number, the design not only facilitates the assembly and disassembly of the magnetic rod sleeves, but also can avoid the overlong structures such as 16-row or 24-row integrated magnetic rod sleeves, and the problems of inconvenient assembly and disassembly, difficulty in assembly and the like caused by the overlong whole magnetic rod sleeves in the using process are avoided. When the extractor is used for extracting a small amount of sample nucleic acid, the corresponding nucleic acid extraction action can be completed only through the independent 8-row magnetic rod sleeves which can be spliced in an end-to-end cascade manner without installing overlong magnetic rod sleeves, the utilization rate of the magnetic rod sleeves is higher, the resource waste is avoided, and the nucleic acid extraction cost is saved.

3. The magnetic rod sleeve realizes the cascade assembly of the head and the tail of each magnetic rod sleeve through the three-end buckle and the cascade ring, has simple structure, low manufacturing cost and more convenient cascade operation, and is suitable for being used in large-scale nucleic acid sampling detection.

4. The four heating modules detachably arranged on each clamping groove of the carrier mechanism are respectively used for heating the plate holes in the 1 st, 6 th, 7 th and 12 th rows of each deep hole plate, so that the deep hole plate can be conveniently disassembled, assembled and replaced, later maintenance is more convenient, and the sample liquid in the specified four rows of plate holes on the same deep hole plate can be preheated and insulated through the four heating modules, so that the effect and the efficiency of preheating and insulating treatment of the sample liquid in the nucleic acid extraction process are improved, and the efficiency of nucleic acid extraction in single operation is further improved.

5. According to the carrier mechanism of the extraction instrument, the Y-axis movement guide of the bracket is realized through the Y-axis slide way assembly, and a user can manually pull the handle to pull out the bracket and the carrier together in practical use, so that the deep hole plate is convenient to disassemble and assemble and the magnetic rod sleeve is convenient to replace, the use is more convenient, and the efficiency of large-batch nucleic acid extraction operation is obviously improved. The carrying platform is movably connected to the bracket through the X-axis slide way assembly, and moves left and right in the X-axis direction under the control of the carrying platform driving motor and the belt transmission assembly, so that the carrying platform driving mechanism is matched to realize cascade driving control on the deep hole plate, the magnetic rod assembly and the magnetic rod sleeve assembly, and further the nucleic acid extraction operation is completed.

6. The stabilizing wheel arranged on the bracket can be rotatably pressed on the bottom plate of the extraction instrument, so that the stability of the bracket when the bracket is manually pulled by a user to move back and forth along the Y-axis direction is ensured, the push-pull operation of the carrying platform is smoother and more stable, and the assembly, disassembly and replacement efficiency of the deep hole plate is favorably improved.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic diagram of the overall structure of the stage mechanism and the deep hole plate of the present invention;

FIG. 3 is a schematic view of the overall structure of the extraction actuator according to the present invention;

FIG. 4 is a schematic view of an overall connection structure of the bar magnet sleeve assembly and the second carrier plate according to the present invention;

FIG. 5 is a schematic view of a bottom structure of a second carrier according to the present invention;

FIG. 6 is a schematic view of a connection structure of the bar magnet sleeve assembly and the card through slot according to the present invention;

FIG. 7 is a schematic view of a magnetic rod sleeve structure according to the present invention;

FIG. 8 is a schematic diagram of the overall rear side structure of the stage mechanism of the present invention;

fig. 9 is a schematic view of the overall bottom structure of the stage mechanism according to the present invention.

In the figure: 1. the system comprises a carrier mechanism, 11 brackets, 111 handles, 12 carriers, 121 clamping grooves, 13Y axial slide assemblies, 14X axial slide assemblies, 15 fixing supports, 16 carrier driving motors, 17 belt transmission assemblies, 2 extraction actuators, 21 first carrier plates, 22 second carrier plates, 221 magnetic rod through hole groups, 222 clamping through grooves, 223 drawing sockets, 23 carrier plate driving mechanisms, 3 deep hole plates, 31 plate holes, 4 bracket assemblies, 5 magnetic rod assemblies, 51 magnetic rods, 6 magnetic rod sleeve assemblies, 61 magnetic rod sleeves, 611 base plates, 612 cascade rings, 613 three-end buckles, 614 sleeves, 615 positioning clamping grooves, 7 heating modules, 8 base plates and 9 stabilizing wheels.

Detailed Description

The structure of the invention will be further described with reference to the accompanying drawings and preferred embodiments of the invention.

Referring to fig. 1 to 3, the alignment sampling mechanism for a nucleic acid extractor of the present invention comprises a stage mechanism 1 disposed in a housing of the extractor, and an extraction actuator 2 correspondingly disposed above the stage mechanism 1.

Specifically, the carrier mechanism 1 is movable along the X-axis and the Y-axis on a horizontal plane, and is provided with 6 locking grooves 121 (as shown in fig. 8) arranged in 3 rows and 2 columns in a matrix manner, each locking groove 121 is detachably locked with a deep hole plate 3, and each deep hole plate 3 is provided with plate holes 31 arranged in 8 rows and 12 columns in a matrix manner, and 96 holes are arranged in the deep hole plate 3.

Specifically, draw actuating mechanism 2 including corresponding the activity through bracket component 4 and locate the first carrier plate 21 of each deep hole board 3 top, swing joint be in bracket component 4 on and correspond the activity be in the second carrier plate 22 of first carrier plate 21 below, and with second carrier plate 22 and first carrier plate 21 linkage connect, be used for ordering about first carrier plate 21 and second carrier plate 22 in the Z axial synchronous or relative carrier plate actuating mechanism 23 who reciprocates.

Specifically, the bottom of the first carrier 21 is arranged in a matrix form with 6 sets of magnetic rod assemblies 5 which are respectively matched with the deep hole plates 3 and arranged in 3 rows and 2 columns, and in match with each other, the second carrier 22 is arranged with 6 sets of magnetic rod sleeve assemblies 6 which respectively correspond to the magnetic rod assemblies 5 and can be movably sleeved outside the magnetic rod assemblies 5 in an inserting, dismounting and mounting manner.

In practical use, the first carrier plate 21 and the second carrier plate 22 are driven by the carrier plate driving mechanism 23 to move in the Z-axis direction, so as to control the magnetic rod sleeve assembly 6 to be sleeved on or separated from the magnetic rod assembly 5 in a linkage manner. The carrier mechanism 1 drives the 6 deep-hole plates 3 clamped on the clamping grooves 121 to horizontally move back and forth or move left and right, so that the magnetic rod sleeve assemblies 6 and the magnetic rod assemblies 5 are in linkage control to complete the alignment sampling operation of each group of magnetic rods and each deep-hole plate 3.

After the technical scheme is adopted, the invention has the following beneficial effects.

Firstly, the stage mechanism 1 of the invention can move along the X-axis direction and the Y-axis direction on the horizontal plane, and in practical use, the stage mechanism 1 can drive the 6 deep-hole plates 3 arranged on the stage mechanism to move back and forth along the Y-axis, so that each deep-hole plate 3 can be exposed in front relative to each magnetic rod sleeve assembly 6 and magnetic rod assembly 5 of the extraction executing mechanism 2, thus facilitating the disassembly and assembly of the deep-hole plates 3 and the replacement operation of the magnetic rod sleeve assemblies 6, and obviously improving the efficiency of the plate replacement operation of the deep-hole plates 3 and the replacement operation of the magnetic rod sleeve assemblies 6 during large-scale nucleic acid sampling detection.

Moreover, the 6 deep-hole plates 3 are clamped in the clamping grooves 121 on the carrier mechanism 1 according to a layout mode of 3 rows and 2 rows, each deep-hole plate 3 is designed by adopting a structure of 8 rows and 12 rows of 96 holes, the layout of the magnetic rod assembly 5 and the magnetic rod sleeve assembly 6 is matched with the layout of the clamping grooves 121, and sample liquid in the 6 deep-hole plates 3 can be simultaneously processed at one time in practical use, so that the sample processing quantity in single operation is obviously improved, the utilization rate of the nucleic acid extractor in unit time is favorably improved, the processing efficiency is improved, and the device is suitable for being used in large-scale nucleic acid sampling detection to improve the extraction efficiency.

In addition, the carrying platform mechanism 1 can be matched with the magnetic rod sleeve assembly 6 and the magnetic rod assembly 5 to move left and right along the X-axis direction, so that the carrying plate driving mechanism 23 is matched to realize accurate and efficient contraposition extraction operation between the magnetic rod and the 12-column plate holes 31 of each deep-hole plate 3, the linkage efficiency of extraction execution actions is high, the contraposition sampling accuracy is high, and the efficiency of nucleic acid extraction is further improved.

The technical solution adopted for further improvement to solve the technical problems proposed by the present invention also includes the following technical features.

Further, referring to fig. 3, each set of the magnetic rod assemblies 5 of the present invention includes 16 total magnetic rods 51 in 8 rows and 2 columns, and the distance between two rows of the magnetic rods 51 of each set of the magnetic rod assemblies 5 matches with the total width of 4 rows of the plate holes on the deep hole plate 3.

And each group of magnetic rod sleeve assemblies 6 comprises 2 parallel magnetic rod sleeves 61 which are spliced in a cascade mode from head to tail and are arranged in 8 rows in a cascade mode.

Further, as shown in fig. 4 to 6, 6 sets of bar magnet through hole sets 221 which are arranged in 3 rows and 2 rows and are respectively matched with the bar magnet assemblies 5 for the vertical penetration of the bar magnets 51 are penetratingly formed on the body of the second carrier 22, and each set of bar magnet through hole sets 221 includes 8 rows of parallel through holes of 2 rows.

Specifically, the bottom of second carrier 22 is provided with 4 longitudinally extending clamping through grooves 222 corresponding to the 8-row through holes in the same row, and the front end of each clamping through groove 222 is provided with a drawing and inserting port 223 located at the front end of second carrier 22.

In practical use, the magnetic rod sleeves 61 can be sequentially inserted into the same clamping through groove 222 through the drawing socket 223, positioned to the corresponding 8-row through holes and cascaded, and can be drawn out through the drawing socket 223 at one time.

After the technical scheme is adopted, 8-row through holes in the same column are connected in series through 4 clamping through grooves 222 on the second carrier plate 22, so that the magnetic rod sleeves 61 of the 8-row structure which can be assembled in a head-to-tail cascade manner can be positioned and assembled into the clamping through grooves 222 one by one through the inserting holes 223 and aligned with the 8-row through holes of the corresponding row number respectively, the design not only facilitates the disassembly and assembly of the magnetic rod sleeves 61, but also can avoid the overlong structures such as 16-row or 24-row integrated magnetic rod sleeves 61, and the problems of inconvenience in disassembly and assembly, difficulty in disassembly and the like caused by the overlong whole magnetic rod sleeves 61 in the using process are avoided.

Moreover, when a 96-channel extractor with the structure is used for extracting a small amount of sample nucleic acid, the integrated magnetic rod sleeves of 16 or 23 rows do not need to be installed too long, corresponding nucleic acid extraction actions can be completed only through the independent magnetic rod sleeves 61 of 8 rows which can be spliced in an end-to-end cascading manner, the utilization rate of the magnetic rod sleeves 61 is higher, resource waste is avoided, and the nucleic acid extraction cost is saved.

Further, referring to fig. 7, the magnetic rod sleeve 61 of the present invention includes a base plate 611, a cascade ring 612, and a three-terminal buckle 613.

Specifically, the base plate 611 is a horizontally and longitudinally extending plate-shaped structure, and 8 sleeves 614 with upper openings on the body are vertically arranged on the base plate at equal intervals. The cascade ring 612 is a closed ring structure and is fixedly disposed at the front end of the substrate 611. The three-end fastener 613 is a slot-combined fastener structure with an opening at the rear end, is fixedly arranged at the tail end of the substrate 611, and can be longitudinally matched and fastened with the cascade ring 612.

Further, as shown in the figure, three sets of elastic bead-pushing structures (not shown in the figure) respectively corresponding to three 8-row through hole regions in the same row are disposed on each of the card-connecting through grooves 222, and positioning slots 615 capable of being engaged with or disengaged from the elastic bead-pushing structures are disposed on two sides of the front portion of the base plate 611 of each magnetic rod sleeve 61.

When the magnetic rod sleeve assembly 6 is actually disassembled and assembled, the front end of an 8-row magnetic rod sleeve 61 with a cascading ring 612 is inserted into the clamping through groove 222 through the drawing socket 223, when the positioning clamping groove 615 on the magnetic rod sleeve 61 is matched and clamped at the elastic top bead structure corresponding to the row of the first row of 8-row through holes, the positioning clamping of the magnetic rod sleeve 61 is completed, at this time, the three-end buckle 613 part at the tail end of the magnetic rod sleeve 61 is positioned at the drawing socket 223, and then the sample liquid on the first row of deep hole plate 3 can be processed through the extraction instrument, so that the extraction operation of a small amount of sample nucleic acid as a 32-channel extraction instrument is realized, and the integrated magnetic rod sleeve of 16 rows or 24 rows does not need to be wasted.

In addition, when samples of two rows or three rows of deep hole plates 3 need to be extracted simultaneously, the cascade ring 612 at the head end of the second 8-row magnetic rod sleeve 61 can be correspondingly clamped to the three-end buckle 613 at the tail end of the previous magnetic rod sleeve 61 at the extraction and insertion port 223, then the two magnetic rod sleeves after cascade assembly are pushed forwards along the clamping through groove 222 by force until the positioning clamping groove 615 on the second magnetic rod sleeve 61 is matched and clamped at the elastic top bead structure of the corresponding row of the first 8-row through holes, so that the positioning clamping of the magnetic rod sleeve 61 is completed, and the two rows of deep hole plate 3 sample liquids are extracted and processed simultaneously. Or, the cascade ring 612 at the head end of the third 8-row magnetic rod sleeve 61 can be correspondingly clamped to the three-end buckle 613 at the tail end of the second magnetic rod sleeve 61 at the extraction and insertion port 223, and then the three magnetic rod sleeves after cascade assembly are forcibly pushed forward along the clamping through groove 222 until the positioning clamping groove 615 on the third magnetic rod sleeve 61 is matched and clamped at the elastic top bead structure corresponding to the first row 8-row through hole, so that the positioning clamping of the magnetic rod sleeve 61 is completed, and the sample liquid of the three-row deep hole plate 3 can be extracted and processed at the same time.

By adopting the technical scheme, the magnetic rod sleeves 61 can realize the end-to-end cascade assembly through the three-end buckle 613 and the cascade ring 612, have simple structure, low manufacturing cost and more convenient cascade operation, and are suitable for large-scale nucleic acid sampling detection.

Moreover, the elastic supporting bead structure and the positioning clamping groove 615 are adopted to match the position of the positioning magnetic rod sleeve 61, the structure is simple, the alignment clamping effect is good, the pulling and inserting movement of the magnetic rod sleeve 61 is facilitated, and the pulling, inserting and dismounting efficiency and the positioning clamping precision of the magnetic rod sleeve 61 are improved.

Further, as shown in fig. 8, each of the clamping grooves 121 of the present invention is detachably connected with four heating modules 7 respectively corresponding to the positions below the plate holes 31 of the 1 st row, the 6 th row, the 7 th row and the 12 th row on the deep hole plate 3 and used for heating the specified plate holes 31.

According to the invention, each heating module 7 on the clamping groove 121 can be conveniently disassembled and replaced, the later maintenance is more convenient, and the sample liquid in the specified four rows of plate holes 31 on the same deep hole plate 3 can be preheated and insulated through the four heating modules 7, so that the effect and efficiency of sample liquid preheating and insulation treatment in the nucleic acid extraction process are improved, and the efficiency of nucleic acid extraction in single operation of the invention is further improved.

Further, as shown in fig. 8 to 9, stage mechanism 1 of the present invention includes a carrier 11, a stage 12, and a stage driving device.

Specifically, the bracket 11 is a rectangular frame structure, is movably arranged on the bottom plate 8 of the extraction instrument through a Y-axis slide way assembly 13, and is provided with a handle 111 at the front end thereof, which can be manually pushed and pulled to push the extraction instrument in or out;

specifically, the carrier 12 is a rectangular horizontal plate structure, and is movably connected to the bracket 11 through an X-axis slide assembly 14, and the card slots 121 are arranged on the upper surface of the carrier 12 in a matrix manner.

Specifically, the stage driving device includes a stage driving motor 16 horizontally and longitudinally fixedly connected to the left position of the rear end face of the bracket 11 through a fixing support 15, and a rotation shaft of the stage driving motor 16 is in transmission connection with the stage 12 through a belt transmission assembly 17, and is used for driving the stage 12 to move left and right in the X axis direction.

In practical use, the bracket 11 is pulled by a user's hand to slide back and forth on a horizontal plane under the limiting action of the Y-axis slide assembly 13, and drives the carrier 12 to move synchronously under the connecting action of the X-axis slide assembly 14, so that the carrier 12 is pulled out to conveniently disassemble and assemble the deep hole plate 3 or the magnetic rod sleeve assembly 6. The carrier 12 is driven by the carrier driving motor 16 and the belt transmission assembly 17 to move horizontally and leftwards and rightwards in the X axis direction under the limiting effect of the X axis slide assembly 14, so that the deep hole plates 3 on the carriers 12 are controlled to move leftwards and rightwards relative to the magnetic rod sleeve assembly 6 and the magnetic rod assembly 5, and the collection and transfer of magnetic beads in the sample liquid are further realized.

After the technical scheme is adopted, the carrier mechanism 1 of the extraction instrument realizes the Y-axis movement guide of the bracket 11 through the Y-axis slide way assembly 13, and a user can manually pull the handle 111 to pull the bracket 11 and the carrier 12 outwards in actual use, so that the deep hole plate 3 is convenient to disassemble and assemble, the use is more convenient, and the efficiency of large-batch nucleic acid extraction operation is obviously improved. The carrying platform 12 is movably connected to the bracket 11 through an X-axis slide way assembly 14 and moves left and right in the X-axis direction under the control of a carrying platform driving motor 16 and a belt transmission assembly 17, so that cascade drive control of the deep hole plate 3, the magnetic rod assembly 5 and the magnetic rod sleeve assembly 6 is realized by matching with a carrying plate driving mechanism 23, nucleic acid extraction operation is further completed, the coordination of the cascade drive control is better, the driving efficiency is higher, and the efficiency of the nucleic acid extraction operation is obviously improved.

Further, referring to fig. 8, the bracket 11 of the present invention is provided with a stabilizer wheel 9 which is rotatably pressed against the bottom plate 8 of the extraction instrument and is used for stabilizing the movement of the bracket 11 in the Y-axis direction.

Specifically, the steady wheel 9 is horizontally and fixedly connected to the rear end face of the bracket 11 and is rotated to abut against the bottom plate 8 of the extraction instrument, so that stability of a user in manually pushing and pulling the handle 111 and driving the bracket 11 and the carrying platform 12 to move back and forth horizontally is guaranteed, pushing and pulling operations of the carrying platform 12 are smoother and more stable, and the deep hole plate 3 can be dismounted and replaced more efficiently.

The above examples are only for the purpose of clarifying the technical solution of the present invention, and are not intended to limit the embodiments of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention, and it is intended that all such modifications and alterations be considered as within the scope of this invention.

Claims

1. A counterpoint sampling mechanism for among nucleic acid extraction appearance which characterized in that includes:

extract actuating mechanism, locate the top of microscope carrier mechanism, including:

2. The alignment sampling mechanism of claim 1, wherein each set of magnetic rod assembly comprises 16 total magnetic rods in 8 rows and 2 columns, and the distance between two rows of magnetic rods in each set of magnetic rod assembly matches with the total width of 4 plate holes on the deep-well plate; each group of magnetic rod sleeve components comprises 2 parallel magnetic rod sleeves which can be spliced in a cascade mode end to end and are arranged in 8 rows.

3. The alignment sampling mechanism of claim 2, wherein 6 sets of magnetic rod through-hole sets are formed through the body of the second carrier, wherein the magnetic rod through-hole sets are arranged in 3 rows and 2 columns and are respectively matched with the magnetic rod assemblies for the vertical penetration of the magnetic rods, and each set of magnetic rod through-hole sets comprises 2 parallel 8 rows of through-holes; the bottom of the second carrier plate is provided with 4 clamping through grooves corresponding to the 8-row through holes in the same row respectively, and the front end of each clamping through groove is provided with a drawing and inserting hole positioned at the front end of the second carrier plate; the magnetic rod sleeves can be sequentially inserted into the same card through groove through the drawing and inserting port, positioned to the corresponding through holes of the 8-row connector and realize cascade connection, and can be drawn out through the drawing and inserting port at one time.

4. The alignment sampling mechanism of claim 3, wherein each of the latch through grooves has three sets of elastic support bead structures corresponding to three of the 8-row through hole regions in the same row, and each of the magnetic rod sleeves has a positioning slot for engaging with or disengaging from the elastic support bead structures.

5. The apparatus as claimed in claim 2, wherein the magnetic rod sleeve comprises:

6. The alignment sampling mechanism of claim 1, wherein each of the positioning grooves is detachably connected with four heating modules for heating the designated plate holes under the plate holes of the deep-well plate in the 1 st, 6 th, 7 th and 12 th rows.

7. The alignment sampling mechanism of claim 1, wherein the stage mechanism comprises:

and the carrying platform driving motor is fixedly arranged on the bracket, is in transmission connection with the carrying platform through a belt transmission assembly and is used for driving the carrying platform to move left and right in the X-axis direction.

8. The aligning and sampling mechanism of claim 7, wherein the bracket has a stabilizing wheel which is rotatably pressed against the bottom plate of the extractor for stabilizing the movement of the bracket in the Y-axis direction.