CN115336558B - Device facilitating observation and measurement of insect biological indexes and feeding and use method - Google Patents
Device facilitating observation and measurement of insect biological indexes and feeding and use method Download PDFInfo
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- CN115336558B CN115336558B CN202211009571.2A CN202211009571A CN115336558B CN 115336558 B CN115336558 B CN 115336558B CN 202211009571 A CN202211009571 A CN 202211009571A CN 115336558 B CN115336558 B CN 115336558B
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- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The invention discloses a device and a using method for facilitating observation and measurement of insect biological indexes and feeding, and relates to the technical field of insect research. Comprises a main body of an insect breeding bin; the lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect-raising bin main body, the bottom copper gauze is respectively connected with the bottom ends of the side gauze lifting mechanism and the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze; the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, wherein the temperature and humidity sensing chip is arranged in the insect breeding bin main body, and the display screen is arranged on the outer side of the insect breeding bin main body and is electrically connected with the temperature and humidity sensing chip; the storage battery is electrically connected with the temperature and humidity reading device. The invention can meet the feeding observation of two species of test insects, and can freely adjust the size of the feeding space.
Description
Technical Field
The invention relates to the technical field of insect research, in particular to a device and a using method for facilitating observation and measurement of insect biological indexes and feeding.
Background
Insects are a good biological research material and are of a wide variety. The insects are used as research objects, and the artificial feeding and observation of the insects are not separated. However, due to the fact that part of insects are smaller in size, the r strategy often causes that the number of insect populations is larger and the generation overlapping phenomenon is serious, so that experimental observation and feeding of single or small numbers of individuals are not facilitated. Therefore, special devices are needed for raising and observing insects, and the design of the devices is in line with the life habits of the insects.
In recent years, the theory and experimental research on competition among insect species is rapid, and the theory and experimental research on competition among insect species is a new hot spot in the ecological research of insects. More and more systematic studies support the principle of competitive exclusion and the Gause theory and confirm that competitive substitution is a frequent occurrence in arthropods of the class insect. Competing substitutions tend to have serious adverse effects on the environment and economy, and many established competing substitutions are due to the invasion of introduced species or foreign species, such as the competing substitution phenomena of bemisia tabaci MED cryptospecies and bemisia tabaci MEAM1 cryptospecies for indigenous species bemisia tabaci. In recent years, with the increasing frequency of economic trade activities and the changing global climate, invasion of foreign species is gradually increased, thereby increasing the frequency of occurrence of competitive substitution. Cases of competing substitutions are also common, with serious economic and ecological impact. Therefore, research on invasion mechanisms of foreign species and mechanisms of competing substitution are particularly important.
At present, the kinds of insect raising equipment are diversified, such as insect raising jar, insect raising box, large-scale insect raising cage etc., can realize raising and propagating different kinds of insects with above insect raising equipment, but most insect raising equipment structure is complicated, is difficult to operate, is only suitable for raising the insect to be unfavorable for observing the insect and survey the going on of experiments such as survey, can appear often because raising the escape of examination worm influences experimental progress and experimental data's accuracy, and then influence the index survey of various experiments in later stage. Most insect breeding cages are bred by adopting 120-mesh gauze cages, so that a certain escape prevention effect is achieved, but the phenomenon of escape of different degrees is found in actual breeding work, and the problem that test data cannot be counted is further solved; therefore, the result of a large amount of feeding work is not ideal, and even the phenomenon of feeding in the middle and test failure occurs. The sizes of the insect breeding cages in the market at present are mostly fixed, the space of the life of insects is fixed, and only one space exists, so that the insect breeding cages are not beneficial to observing indexes of competition of two insects in the same space, and are also not beneficial to observing the quantity change of insect populations in different spaces; meanwhile, the internal volume of the insect breeding cage cannot be changed, so that different numbers of insects cannot be bred, and when the insect amount is small, the insect breeding cage is relatively large in space, so that insect bodies are scattered, and observation is inconvenient.
Therefore, it is necessary to design a device which can separate and separately feed and observe two insects at the same time, and the size of the feeding space is adjustable, thereby being beneficial to observing and measuring biological indexes of insects and feeding.
The device is convenient to operate, is easy to observe, reduces the labor intensity, and solves the problems that the existing insect breeding device can not meet the requirements of two insect test breeding observations at the same time and can freely adjust different space sizes.
Disclosure of Invention
In order to solve the technical problems, the invention adopts the following technical scheme:
device that does benefit to observation survey insect biological index and raise includes:
a pest-culturing cabin main body;
the lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect-raising bin main body, the bottom copper gauze is respectively connected with the bottom ends of the side gauze lifting mechanism and the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze;
the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, wherein the temperature and humidity sensing chip is arranged inside the insect breeding bin main body, and the display screen is arranged outside the insect breeding bin main body and is electrically connected with the temperature and humidity sensing chip;
and the storage battery is electrically connected with the temperature and humidity reading device.
Further, be provided with two cavities in the worm raising bin main part, all be provided with in every cavity separate the gauze elevating system, separate the gauze elevating system including separate gauze lifting reel and separate the gauze, separate the gauze lifting reel with separate the gauze all be provided with two, two separate gauze lifting reel parallel arrangement, and two separate the gauze lifting reel respectively swivelling joint in the top of two adjacent lateral walls of cavity, two separate the gauze respectively connect in two separate on the gauze lifting reel.
Further, the side gauze lifting mechanism comprises side gauze lifting reels and side gauze, the side gauze lifting reels are provided with six groups, the six groups of side gauze lifting reels are respectively and rotatably connected to the inner walls of the two chambers, which are not provided with separation gauze lifting mechanisms, and the six groups of side gauze lifting reels are respectively connected with side gauze.
Further, still include host plant fixing device, host plant fixing device includes fixation clamp, expansion sheet and draws the bin gate that contracts, draw the bin gate connect in the insect rearing bin main part top that contracts, draw and be provided with the pore on the bin gate that contracts, the fixation clamp connect in the insect rearing bin main part keep away from draw on the outer wall of bin gate one side that contracts, expansion sheet sliding connection in on the fixation clamp, the expansion sheet runs through and alternates insect rearing bin main part and extend to inside the insect rearing bin main part.
Further, the insect breeding bin also comprises a nutrition device, wherein the nutrition device comprises a nutrition liquid pipe, and the nutrition liquid pipe is connected to the side wall of the insect breeding bin main body and is positioned at the lower side of the drawing bin door.
Further, the insect discharging device comprises an insect discharging hole and a plastic plug, wherein the insect discharging hole is formed in the side wall of the insect culturing bin main body, and the plastic plug is detachably connected with the insect discharging hole.
Further, still include the fodder and put and get the device, the fodder is put and is got the device and include fodder bin gate and fodder dish, the fodder bin gate with the worm storehouse main part is articulated, the fodder dish sets up in the worm storehouse main part inside.
Further, the insect breeding bin also comprises a ventilation device, wherein the ventilation device comprises ventilation holes uniformly formed in the top of the insect breeding bin main body.
Further, the insect breeding bin main body is made of transparent glass materials, and scale marks are arranged on the outer wall of the insect breeding bin main body.
The application method of the device for observing and measuring insect biological indexes and raising is beneficial to the observation and measurement, and comprises the following steps:
step one: firstly, opening a retractable bin gate, putting the picked host plants into a main body of the insect breeding bin, expanding blades of the host plants, and properly adjusting the length of the telescopic piece on the fixing clamp according to the size of the blades to enable the telescopic piece to clamp and fix the blade tips of the blades of the host plants on the top of the main body of the insect breeding bin;
step two: the leaf stalks of the host plants extend out of the drawing and shrinking bin gate, the drawing and shrinking bin gate is put down, so that the leaf stalks of the host plants pass through the pore canal in the middle of the lower edge of the drawing and shrinking bin gate and are tightly attached by absorbent cotton;
step three: the petioles of the host plants are put into a nutrient solution pipe, and the prepared nutrient solution is poured into the nutrient solution pipe;
step four: pulling a feed bin door of the feed placing and taking device open, placing prepared feed in a feed tray, placing the prepared feed from the feed tray onto copper yarns at the bottom of the insect raising bin main body, and closing the feed bin door after all the feed is placed;
step five: opening the insect-killing device, pulling up the plastic plug on the insect-killing hole, adjusting the mouth of the insect-killing tube to enable the mouth of the insect-killing tube to correspond to the insect-killing hole of the insect-killing device, tapping the insect-killing tube, driving insects in the insect-killing tube into the insect-killing device, and quickly plugging the plastic plug after all insects to be tested are put into the device;
step six: the gauze is fixed in a set space, so that the test insects can stay on the blades as soon as possible, and when the test insects are stabilized on the host plants, the scale of the gauze is adjusted according to the test requirements;
step seven: if two kinds of insects are bred and observed, the partition gauze which is separated in the middle of the insect breeding bin main body is subjected to lifting control, and is adjusted to a proper scale together with other gauze;
step eight: observing the temperature and humidity values of the hygrothermograph;
step nine: adding a proper amount of nutrient solution according to the reduction amount of the nutrient solution in the nutrient solution pipe;
step ten: and after the steps are finished, starting a new round of insect test feeding.
The invention has the beneficial effects that:
the device which is beneficial to observing and measuring the insect biological index and raising has simple and practical structure and high repeatability; the space of each side wall gauze and the bottom copper gauze in the device is changed by controlling the lifting of the side wall gauze and the bottom copper gauze by a motor, and the space is divided into two parts by adjusting the lifting height to realize the feeding and the observation of two insects at the same time. If the middle gauze is lifted to the top of the insect-raising device, the insect-raising chamber becomes an independent space, the lifting gauze is fixed on the appointed scale mark, and two insects are placed in the fixed space, so that indexes of biological characteristics of the two insects and competition relations of the insects in different spaces can be conveniently observed; the nutrient solution pipe on the outer wall is convenient for adding host plant nutrient solution, maintains the freshness of the leaves, reduces the replacement of the leaves, and is simple and convenient to operate; the temperature and humidity reading device can observe the temperature and humidity in the life space of the test insects at any time, can properly adjust according to the life habit of the test insects, and can know indexes such as the growth and development calendar of the test insects under different temperature and humidity conditions.
Drawings
FIG. 1 is a schematic diagram of an apparatus for facilitating observation and measurement of insect biological indicators and feeding according to the present invention.
FIG. 2 is a schematic diagram of a nutrient solution tube of the device for observing and measuring biological indexes of insects and feeding according to the invention.
FIG. 3 is a schematic diagram showing the cooperation of nutrient solution tubes and a retractable bin gate of the device for observing and measuring biological indexes of insects and feeding according to the invention.
FIG. 4 is a schematic view of a lifting switch of the device for observing and measuring biological indexes of insects and raising the insects.
FIG. 5 is a schematic view of a wet thermometer of the apparatus of the present invention for facilitating the observation and measurement of insect biological indicators and feeding.
FIG. 6 is a schematic view showing the rolling state of the lifting reel of the separating gauze of the device for observing and measuring insect biological indexes and feeding according to the present invention.
FIG. 7 is a schematic view showing a partially lowered state of the spacer screen lifting reel of the device for observing and measuring biological indexes of insects and raising the insects according to the present invention.
FIG. 8 is a schematic view of a mounting clip for a device for facilitating the observation and measurement of biological indicators and feeding of insects in accordance with the present invention.
Fig. 9 is a graph showing the survival rate of various number of bemisia tabaci in different space sizes during one day in the third example.
Fig. 10 is a graph showing the survival rate of varying numbers of bemisia tabaci in varying space sizes over three days in example three.
Fig. 11 is a graph showing the survival rate of varying numbers of bemisia tabaci in varying space sizes over seven days in example three.
Wherein, in the figure:
1-a main body of the insect breeding bin; 2-a nutrient solution tube; 3-fixing clips; 4-pulling and shrinking bin gate; 5-pore canal; 6-a worm hole; 7-a feed bin gate; 8-side gauze; 9-lifting switch; 10-side gauze lifting reel; 11-a display screen; 12-ventilation holes; 13-separating gauze lifting reel, 14-separating gauze and 15-telescopic sheet.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 11 of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The device for observing and measuring insect biological indexes and raising is provided by combining with fig. 1-8, and comprises an insect raising bin main body 1, a lifting gauze device, a temperature and humidity reading device and a storage battery, wherein the lifting gauze device, the temperature and humidity reading device and the storage battery are all connected to the insect raising bin main body 1, and the storage battery is electrically connected with the temperature and humidity reading device.
The lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect-raising bin main body 1, the bottom copper gauze is respectively connected with the side gauze lifting mechanism and the bottom end of the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze.
The utility model discloses a worm raising bin, including breeding worm storehouse main part, be provided with two cavities in the worm storehouse main part, all be provided with in every cavity separate gauze elevating system, separate gauze elevating system including separate gauze elevating spool 13 and separate gauze 14, separate gauze elevating spool 13 with separate gauze 14 all is provided with two, two separate gauze elevating spool 13 parallel arrangement, and two separate gauze elevating spool 13 swivelling joint respectively in the top of two cavity adjacent lateral walls, two separate gauze 14 connect respectively on two separate gauze elevating spool 13.
The side gauze lifting mechanism comprises a side gauze lifting scroll 10 and side gauze 8, the side gauze 8 lifting scroll 10 is provided with six groups, the six groups of side gauze 8 lifting scroll 10 are respectively and rotatably connected with the two inner wall top ends of the chamber, which are not provided with the separation gauze lifting mechanism, the six groups of side gauze 8 lifting scroll 10 are respectively connected with the side gauze 8, so that the side gauze 8 lifting scroll 10 drives the separation gauze lifting scroll 13 to rotate, and the lifting of the separation gauze 14 is realized.
In the embodiment, the side gauze 8 and the separation gauze 14 are both 150-mesh gauze, so that small insects cannot drill out, and the method is safe and reliable; the copper yarn at the bottom adopts 150 meshes of copper yarn, is firm and reliable, and can support the feed and feed tray for feeding insects.
As an optimization, in this embodiment, the side gauze lifting reel 10 and the partition gauze lifting reel 13 adopt a motor driving mode, each motor is electrically connected with a storage battery, the storage battery provides electric energy for each motor, each motor is provided with a lifting switch 9 for controlling the motor to operate, and the lifting switch 9 is connected to the outer side of the insect raising bin main body 1 and electrically connected with the corresponding motor, so that the use safety performance of the motor is improved.
Furthermore, the lifting switch 9 is made of waterproof materials, so that the lifting switch 9 is prevented from malfunctioning due to a field humid environment.
In other embodiments, the side screen lifting reels 10 and the spacer screen lifting reels 13 are rotated by manual actuation.
The temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen 11, wherein the temperature and humidity sensing chip is arranged inside the insect breeding bin main body 1, an insulating sheet is arranged on the temperature and humidity sensing chip, the influence of potential hazards such as electric leakage on test insects in the insect breeding bin main body is avoided, and the display screen 11 is arranged outside the insect breeding bin main body 1 and is electrically connected with the temperature and humidity sensing chip; the setting of display screen makes can be on the screen direct display number of degrees, more conveniently reads temperature and humidity in the worm storehouse main part, observes temperature and air humidity condition in the test worm living space at any time to according to test worm life habit and experimental needs can carry out suitable physical regulation.
Further, the device beneficial to observing and measuring insect biological indexes and feeding further comprises a host plant fixing device and a nutrition device, wherein the host plant fixing device comprises a fixing clamp 3, a telescopic piece 15 and a telescopic bin door 4, the telescopic bin door 4 is connected to the top end of the insect feeding bin main body 1, a duct 5 is arranged on the telescopic bin door 4, the fixing clamp 3 is connected to the outer wall of one side, away from the telescopic bin door 4, of the insect feeding bin main body 1, the telescopic piece 15 is connected to the fixing clamp 3 in a sliding manner, and the telescopic piece 15 penetrates through the insect feeding bin main body 1 and extends into the insect feeding bin main body 1; the nutrition device comprises a nutrition liquid pipe 2, wherein the nutrition liquid pipe 2 is connected to the side wall of the insect rearing bin main body 1 and is positioned at the lower side of the drawing bin door 4; the petioles of the host plants pass through the pore canal and extend into the nutrient solution pipe, so that soilless culture of the host plants is realized.
In this embodiment, the two sets of the host plant fixing devices are respectively located in two spaces formed by separating the partition gauze, the telescopic piece 15 on the fixing clamp can extend into the insect-cultivating room for fixing the leaf tips of the host plants, the maximum length of the telescopic piece 15 is 15cm, the telescopic piece 15 can be stretched and fixed according to the sizes of the leaves of the host plants, so that the leaves of the host plants are flatly paved on the top of the insect-cultivating device, the fixing of the leaves of the host plants with various sizes is satisfied, and the insect-cultivating and egg-laying are facilitated; the nutrient solution tube adopts a semi-cylindrical test tube with the diameter of 20mm and made of transparent plastic, the liquid volume scale is arranged on the semi-cylindrical test tube, the maximum scale is 10ml, the setting of the scale better controls the preparation amount of the nutrient solution, the preparation amount of the nutrient solution can be determined according to the scale on the nutrient solution tube, and the waste is reduced; the middle position of the lower edge of the drawing and shrinking bin gate right above the nutrient solution pipe is semicircular, the diameter of the pore canal is 10mm, enough space is reserved for the leaf stalks of the host plants, the leaf stalks of the host plants are not damaged, the survival time of the host plants is prolonged, and the leaf stalks of the host plants can be fixed by matching absorbent cotton.
Further, the insect discharging device comprises an insect discharging hole 6 and a plastic plug, wherein the insect discharging hole 6 is arranged on the side wall of the insect breeding bin main body 1, and the plastic plug is detachably connected with the insect discharging hole 6; in the embodiment, the diameter of the worm discharge hole is 20mm, the gauze corresponding to the worm discharge hole is also provided with holes with the same size as the worm discharge hole, and rubber rings are arranged around the holes, so that the sealing performance of the plastic plug is better, and the escape of test insects is avoided.
Further, the device also comprises a feed placing and taking device, wherein the feed placing and taking device comprises a feed bin gate 7 and a feed tray, the feed bin gate 7 is hinged with the insect breeding bin main body 1, and the feed tray is arranged in the insect breeding bin main body 1; in this embodiment, the feed bin gate 7 is 10cm long and 3cm high, and the handle is arranged in the middle of the feed bin gate 7, so that the setting of the feed taking and placing device can meet the requirements of different test insects on different feeds, and not only can natural feeds of host plants be placed, but also artificial feeds can be placed, so that most suitable feeds can be provided for test insects.
As the optimization, still include ventilation unit, ventilation unit including evenly set up in the bleeder vent 12 at worm storehouse main part 1 top, the diameter of bleeder vent is 1mm in this embodiment, is provided with four row circular shape bleeder vents 12 altogether, and every row includes 10 circular shape bleeder vents 12, every row bleeder vent interval 5cm, every row bleeder vent interval 3cm, increases the ventilation in the worm storehouse main part through the setting of a plurality of bleeder vents.
The insect breeding bin body 1 is made of transparent glass materials, scale marks are arranged on the outer wall of the insect breeding bin body 1, the height of lifting of the gauze can be controlled more clearly through the arrangement of the scale marks, the survival space of the insects is changed, and accordingly observation of the competition relationship of the insects in different spaces is met.
The device which is beneficial to observing and measuring the insect biological index and raising has simple and practical structure and high repeatability; the space of each side wall gauze and the bottom copper gauze in the device is changed by controlling the lifting of the side wall gauze and the bottom copper gauze by a motor, and the space is divided into two parts by adjusting the lifting height to realize the feeding and the observation of two insects at the same time. If the middle gauze is lifted to the top of the insect-raising device, the insect-raising chamber becomes an independent space, the lifting gauze is fixed on the appointed scale mark, and two insects are placed in the fixed space, so that indexes of biological characteristics of the two insects and competition relations of the insects in different spaces can be conveniently observed; the nutrient solution pipe on the outer wall is convenient for adding host plant nutrient solution, maintains the freshness of the leaves, reduces the replacement of the leaves, and is simple and convenient to operate; the temperature and humidity reading device can observe the temperature and humidity in the life space of the test insects at any time, can properly adjust according to the life habit of the test insects, and can know indexes such as the growth and development calendar of the test insects under different temperature and humidity conditions.
Example two
The application method of the device for observing and measuring insect biological indexes and raising is beneficial to the observation and measurement, and comprises the following steps:
step one: firstly, opening a retractable bin gate, putting the picked host plants into a main body of the insect breeding bin, expanding blades of the host plants, and properly adjusting the length of the telescopic piece on the fixing clamp according to the size of the blades to enable the telescopic piece to clamp and fix the blade tips of the blades of the host plants on the top of the main body of the insect breeding bin;
step two: the leaf stalks of the host plants extend out of the drawing and shrinking bin gate, the drawing and shrinking bin gate is put down, so that the leaf stalks of the host plants pass through the pore canal in the middle of the lower edge of the drawing and shrinking bin gate and are tightly attached by absorbent cotton;
step three: the petioles of the host plants are put into a nutrient solution pipe, and the prepared nutrient solution is poured into the nutrient solution pipe;
step four: pulling a feed bin door of the feed placing and taking device open, placing prepared feed in a feed tray, placing the prepared feed from the feed tray onto copper yarns at the bottom of the insect raising bin main body, and closing the feed bin door after all the feed is placed;
step five: opening the insect-killing device, pulling up the plastic plug on the insect-killing hole, adjusting the mouth of the insect-killing tube to enable the mouth of the insect-killing tube to correspond to the insect-killing hole of the insect-killing device, tapping the insect-killing tube, driving insects in the insect-killing tube into the insect-killing device, and quickly plugging the plastic plug after all insects to be tested are put into the device;
step six: the gauze is fixed in a set space, so that the test insects can stay on the blades as soon as possible, and when the test insects are stabilized on the host plants, the scale of the gauze is adjusted according to the test requirements;
step seven: if two kinds of insects are bred and observed, the partition gauze which is separated in the middle of the insect breeding bin main body is subjected to lifting control, and is adjusted to a proper scale together with other gauze;
step eight: observing the temperature and humidity values of the hygrothermograph;
step nine: adding a proper amount of nutrient solution according to the reduction amount of the nutrient solution in the nutrient solution pipe;
step ten: and after the steps are finished, starting a new round of insect test feeding.
Example III
The present example uses a device that facilitates the observation and measurement of insect biological indicators and feeding.
1. Selecting a tested insect source and testing operation:
the test selects the sample land planted with different crops for observation and feeding test, adopts the device which is favorable for observation and measurement of insect biological indexes and feeding, and adopts the self-sealing bag and the insect rearing cage for observation and feeding.
(1) Comparison of the survival time of different numbers of insects on different host plants
TABLE 1 different numbers of insects on different host plants for survival time
Note that: the survival time values in the table are mean ± standard error; the same column of different lowercase letters indicates that the same insect under different host plants has a significant difference in survival time under different devices. (Tukey HSD test, P < 0.05)
The survival times of the bemisia tabaci on different host plants raised in different numbers are shown in table 1, and bemisia tabaci collected from tomatoes, bemisia tabaci collected from cucumbers, bemisia tabaci collected from eggplants were raised in the same 20 head numbers. 20 bemisia tabaci are collected from tomatoes, the survival time is 19.67d, the self-sealing bag is 3.67d, and the insect breeding cage 9d (F) 2,8 =380.2624, p=0.0001). 20 bemisia tabaci collected from cucumber, the survival time period of the bemisia tabaci is 18.67d, the self-sealing bag is 3.33d, and the insect cage is 9.33d (F) 2,8 187.4160, p=0.0001). 20 bemisia tabaci are collected from eggplants, the invention is used for feeding and survival time length is 19.67d, self-sealing bag 4d and insect breeding cage 9.67d (F 2,8 =313.4194, p=0.0001). The survival time from the collection of 40 bemisia tabaci on tomatoes to the feeding of the invention is 17.67 days, the self-sealing bag is 2.67 days, the insect breeding cage is 6.00 days (F) 2,8 =145.3110, p=0.0001). Collecting 40 bemisia tabaci from cucumber, and keeping the bemisia tabaci for 16.67 days until the breeding survival time of the invention, wherein the self-sealing bag is 2.33 days, the insect breeding cage is 5.67 days (F 2,8 =304.7616, p=0.0001). Collecting 40 bemisia tabaci from eggplant until the survival time of the breeding of the invention is 17.33 days, the self-sealing bag is 2.00 days, the insect breeding cage is 5.33 days (F 2,8 =366.6442, p=0.0001). 60 bemisia tabaci are collected from tomatoes until the breeding survival time of the invention is 15.67d, the self-sealing bag is 1.67d, and the insect breeding cage is 2.d (F) 2,8 =722.2215, p=0.0001). 60 bemisia tabaci are collected from cucumbers, the survival time is 15.33 days from the feeding of the invention, the self-sealing bag is 1.33 days, the insect breeding cage is 2.67 days (F) 2,8 =309.4408, p=0.0001). 60 bemisia tabaci are collected from eggplants, the survival time from the feeding of the invention is 15.00 days, the self-sealing bag is 2.00 days, the insect breeding cage is 2.33 days (F) 2,8 288.9082, p=0.0001). The survival time from the collection of 80 bemisia tabaci from tomato to the feeding of the invention is 11.67 days, the self-sealing bag is 0.667 days, the insect breeding cage is 1.33 days (F) 2,8 ＝418.9303, p=0.0001). The survival time from the collection of 80 bemisia tabaci from cucumber to the feeding of the invention is 12.33 days, the self-sealing bag is 0.333d, the insect breeding cage is 1.00d (F) 2,8 =146.1670, p=0.0001). The survival time from the collection of 80 bemisia tabaci from the eggplant to the feeding of the invention is 12.67d, the self-sealing bag is 0.667d, the insect breeding cage is 1.33d (F) 2,8 =211.4084, p=0.0001). From the table, it can be seen that the difference of the survival time of the insects collected from different plants in the invention, the self-sealing bags and the insect raising cages is remarkable, the survival time of the insects in the invention is greatly prolonged compared with that of the self-sealing bags and the insect raising cages, and the survival time of the insects in the invention is greatly prolonged along with the reduction of the number of the insects in the invention.
Note that: the F value is the statistic of F test, that is, the ratio of sum of squares of dispersion and degree of freedom between groups and within groups, the significance is the significance level corresponding to the F statistic, and 0.001 indicates that the original assumption is rejected, that is, there is a significant difference between different levels of single factors, F (degree of freedom between groups, degree of freedom within groups) =specific value, in single factor analysis of variance, degree of freedom between groups is r-1, degree of freedom within groups is n-r, where r is the grouping level number of factors, and n is the total sample number. The P value is also called to check whether the P value is the intensity of the original hypothesis, the principle of the P value is that the overall hypothesis is the original hypothesis, a sample is extracted, under the condition of the original hypothesis, the probability value of the sample is the probability value of the small probability event rejecting the original hypothesis according to the principle that the small probability event cannot occur in one experiment, and the smaller the value is, the more unlikely the original hypothesis is, and the significance level of the original hypothesis can be also rejected.
(2) Survival rate comparison of different number of Bemisia tabaci in different space sizes
The present example shows the survival rate of different number of bemisia tabaci raised and observed in different spaces on the same day, and the first day, as shown in FIG. 9, is the survival rate of different number of bemisia tabaci in different space sizes (1 day), when the space of the apparatus is 300cm 3 When 20 bemisia tabaci is raised, the survival rate is 86.7%, 40 is raised, the survival rate is 68.3%, and 60 is raised, the survival rate is 51.1% (F) 2,8 = 238.9252, p=0.0285). When the space of the device is 450cm 3 When raising 20 heads of tobacco powderThe survival rate of the lice was 93.3%, the survival rate of the 40 heads raised was 78.3%, the survival rate of the 60 heads raised was 62.8% (F) 2,8 =94.7709, p=0.0285). When the space of the device is 600cm 3 When 20 bemisia tabaci was bred, the survival rate was 96.7%, 40 was 88.3%, and 60 was 75.0% (F) 2,8 =21.8655, p=0.0020). On the third day, as shown in FIG. 10, the survival rate (3 days) of different number of bemisia tabaci in different space size was 300cm when the space of the device was 300cm 3 When 20 bemisia tabaci was bred, the survival rate was 73.3%, 40 was 57.5%, 60 was 42.2% (F) 2,8 =1.2634, p= 0.3838). When the space of the device is 450cm 3 When 20 bemisia tabaci is raised, the survival rate is 90.0%, 40 is raised, the survival rate is 66.7%, and 60 is raised, the survival rate is 52.2% (F) 2,8 = 113.2979, p=0.0001). When the space of the device is 600cm 3 When 20 bemisia tabaci is raised, the survival rate is 93.3%, the survival rate is 81.7% for 40, and the survival rate is 64.4% (F) 2,8 =40.1385, p=0.0004). On the seventh day, as shown in FIG. 11, the survival rate (7 days) of different number of bemisia tabaci in different space size was 300cm in the present apparatus 3 When 20 bemisia tabaci is raised, the survival rate is 65.0%, the survival rate is 45.0%, and the survival rate is 31.1% (F) 2,8 =14.0498, p=0.0001). When the space of the device is 450cm 3 When 20 bemisia tabaci is raised, the survival rate is 80.0%, the survival rate is 60.0% for 40, and the survival rate is 42.2% (F) 2,8 = 113.0117, p=0.0003). When the space of the device is 600cm 3 When 20 bemisia tabaci was bred, the survival rate was 83.3%, 40 was bred, the survival rate was 70.8%, and 60 was bred, the survival rate was 51.1% (F) 2,8 =102.0596, p=0.0001). The figure shows that the different numbers of insects have obvious difference of the survival rate in different spaces of the invention, the space is unchanged, the survival rate is reduced along with the increase of the insect quantity, and the survival rate of the insects in the invention is obviously improved along with the increase of the survival space of the insects.
In this embodiment, the values in the graphs of fig. 9, 10 and 11 are mean ± standard error; different lowercase letters on the panels indicate that different numbers of bemisia tabaci harvested on the same host plant have significant differences in survival at different spatial sizes on the same day (Tukey HSD test, P < 0.05).
Experiments in this embodiment show that the device for observing and measuring insect biological indexes and raising the insects has very obvious advantages in specific experiments and ensuring insect survival rate, and different insect quantity survival rates are raised in different spaces, so that the survival rate of the insects can be ensured by adjusting the most suitable space size of the raised insect quantity, and the survival rate of the insects is far lower than that of the device for observing and measuring the insect biological indexes and raising the insects by utilizing the self-sealing bags and the insect raising cages.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the embodiments described above will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (3)
1. Device that does benefit to observation survey insect biological index and raise, characterized in that includes:
a pest-culturing cabin main body;
the lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect-raising bin main body, the bottom copper gauze is respectively connected with the bottom ends of the side gauze lifting mechanism and the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze;
the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, wherein the temperature and humidity sensing chip is arranged inside the insect breeding bin main body, and the display screen is arranged outside the insect breeding bin main body and is electrically connected with the temperature and humidity sensing chip;
the storage battery is electrically connected with the temperature and humidity reading device;
the insect breeding bin comprises an insect breeding bin body, wherein two chambers are arranged in the insect breeding bin body, each chamber is internally provided with a separation gauze lifting mechanism, each separation gauze lifting mechanism comprises a separation gauze lifting scroll and a separation gauze, two separation gauze lifting scroll are arranged in parallel, the two separation gauze lifting scroll are respectively and rotatably connected to the top ends of adjacent side walls of the two chambers, and the two separation gauze lifting scroll are respectively connected to the two separation gauze lifting scroll;
the side gauze lifting mechanism comprises side gauze lifting reels and side gauze, six groups of side gauze lifting reels are arranged, the six groups of side gauze lifting reels are respectively and rotatably connected to the inner walls of the two chambers, on which no partition gauze lifting mechanism is arranged, and the six groups of side gauze lifting reels are connected with side gauze;
the insect breeding bin comprises an insect breeding bin body, and is characterized by further comprising a host plant fixing device, wherein the host plant fixing device comprises a fixing clamp, a telescopic piece and a telescopic bin door, the telescopic bin door is connected to the top end of the insect breeding bin body, a pore canal is formed in the telescopic bin door, the fixing clamp is connected to the outer wall of one side, far away from the telescopic bin door, of the insect breeding bin body, the telescopic piece is connected to the fixing clamp in a sliding manner, and the telescopic piece penetrates through the insect breeding bin body and extends into the insect breeding bin body;
the nutrition device comprises a nutrition liquid pipe which is connected to the side wall of the insect rearing bin main body and is positioned at the lower side of the drawing bin door;
the insect placing device comprises an insect placing hole and a plastic plug, wherein the insect placing hole is formed in the side wall of the insect breeding bin main body, and the plastic plug is detachably connected with the insect placing hole;
the device comprises a feed bin door and a feed tray, wherein the feed bin door is hinged with the insect breeding bin main body, and the feed tray is arranged in the insect breeding bin main body;
the application method of the device for observing and measuring insect biological indexes and raising is beneficial to the observation and measurement, and comprises the following steps:
step one: firstly, opening a retractable bin gate, putting the picked host plants into a main body of the insect breeding bin, expanding blades of the host plants, and properly adjusting the length of the telescopic piece on the fixing clamp according to the size of the blades to enable the telescopic piece to clamp and fix the blade tips of the blades of the host plants on the top of the main body of the insect breeding bin;
step two: the leaf stalks of the host plants extend out of the drawing and shrinking bin gate, the drawing and shrinking bin gate is put down, so that the leaf stalks of the host plants pass through the pore canal in the middle of the lower edge of the drawing and shrinking bin gate and are tightly attached by absorbent cotton;
step three: the petioles of the host plants are put into a nutrient solution pipe, and the prepared nutrient solution is poured into the nutrient solution pipe;
step four: pulling a feed bin door of the feed placing and taking device open, placing prepared feed in a feed tray, placing the prepared feed from the feed tray onto copper yarns at the bottom of the insect raising bin main body, and closing the feed bin door after all the feed is placed;
step five: opening the insect-killing device, pulling up the plastic plug on the insect-killing hole, adjusting the mouth of the insect-killing tube to enable the mouth of the insect-killing tube to correspond to the insect-killing hole of the insect-killing device, tapping the insect-killing tube, driving insects in the insect-killing tube into the insect-killing device, and quickly plugging the plastic plug after all insects to be tested are put into the device;
step six: the gauze is fixed in a set space, so that the test insects can stay on the blades as soon as possible, and when the test insects are stabilized on the host plants, the scale of the gauze is adjusted according to the test requirements;
step seven: if two kinds of insects are bred and observed, the partition gauze which is separated in the middle of the insect breeding bin main body is subjected to lifting control, and is adjusted to a proper scale together with other gauze;
step eight: observing the temperature and humidity values of the temperature and humidity reading device;
step nine: adding a proper amount of nutrient solution according to the reduction amount of the nutrient solution in the nutrient solution pipe;
step ten: and after the steps are finished, starting a new round of insect test feeding.
2. The device for facilitating observation and measurement of biological indicators and feeding of insects according to claim 1, further comprising a ventilation device, wherein the ventilation device comprises ventilation holes uniformly formed in the top of the insect rearing bin body.
3. The device for facilitating observation and measurement of insect biological indexes and feeding according to claim 1, wherein the insect breeding bin main body is made of transparent glass materials, and graduation marks are arranged on the outer wall of the insect breeding bin main body.
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| CN105828051A (en) * | 2016-05-30 | 2016-08-03 | 中国农业科学院植物保护研究所 | Real-time small insect behavior monitoring system and application thereof |
| CN108902056A (en) * | 2018-07-09 | 2018-11-30 | 云南马蜂窝农业科技有限公司 | A kind of wasp cultivation bee canopy can change spatial area |
| CN208572919U (en) * | 2018-07-25 | 2019-03-05 | 中国热带农业科学院环境与植物保护研究所 | A kind of simple dependent insect cage for field live plant |
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| WO2021214375A1 (en) * | 2020-04-24 | 2021-10-28 | Entocube Oy | Method and system for rearing insects |
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| CN210900989U (en) * | 2019-09-10 | 2020-07-03 | 山东临沂烟草有限公司 | Armoracia chinensis breeding device |
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