CN115336558A - Device beneficial to observing and measuring biological indexes of insects and feeding and using method - Google Patents
Device beneficial to observing and measuring biological indexes of insects and feeding and using method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a device for facilitating observation and measurement of biological indexes of insects and feeding and a using method thereof, and relates to the technical field of insect research. Comprises a main body of the insect breeding bin; the lifting gauze device comprises a side gauze lifting mechanism, a separating gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separating gauze lifting mechanism are connected to the insect breeding bin main body; the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, wherein the temperature and humidity sensing chip is installed inside the insect breeding cabin body, and the display screen is installed on the outer side of the insect breeding cabin body and is electrically connected with the temperature and humidity sensing chip; the storage battery is electrically connected with the temperature and humidity reading device. The invention can meet the feeding observation of two kinds of test insects and can freely adjust the feeding space.
Description
Technical Field
The invention relates to the technical field of insect research, in particular to a device for facilitating observation and measurement of biological indexes of insects and feeding and a using method thereof.
Background
Insects are a wide variety of good biological research materials. The insects are used as research objects, and artificial feeding and observation of the insects can not be avoided. However, because some insects have smaller body types and the r strategy usually results in a larger number of insect populations and serious generation overlapping phenomenon, the experimental observation and feeding of a single or a small number of individuals are not facilitated. Therefore, the feeding and observation of the insects need to adopt a special device, and the design of the device is in accordance with the living habit of the insects.
Theoretical and experimental studies on inter-species competition of insects have been rapidly developed in recent years, and have become a new hot spot in the study of insect ecology. An increasing number of systematic studies support the principle of competitive exclusion and the gauss theory and confirm that competitive replacement is a frequently occurring phenomenon in arthropods of the class insecta. Competitive substitution often has serious negative effects on the environment and economy, and many established competitive substitutions are caused by the introduction of species or invasion of foreign species, such as the phenomenon of competitive substitution of bemisia tabaci MED cryptovariety and bemisia tabaci mema 1 cryptovariety for indigenous species of bemisia tabaci. In recent years, with the increasing frequency of economic trade activities and global climate change, the invasion of foreign species is gradually increased, and thus the frequency of competitive substitution is increased. Competitive substitution cases are also common, with serious economic and ecological impacts. Therefore, it is important to study the invasion mechanism and competitive substitution mechanism of foreign species.
At present, the kind of insect raising equipment is diversified, for example, insect raising jars, insect raising boxes, large-scale insect raising cages and the like, and different kinds of insects can be raised and propagated by utilizing the insect raising equipment, but most of the insect raising equipment are complex in structure and difficult to operate, are only suitable for raising the insects, are not beneficial to observing and measuring the insects, and are often influenced by the escape of raising test insects, the accuracy of test progress and test data, and further the index measurement of various later-stage tests. Most of the insect cages are fed by 120-mesh gauze cages, so that a certain escape prevention effect is achieved, but the phenomenon of escape to different degrees still exists in the actual feeding work, and the problem that test data cannot be counted is caused; therefore, the results of a large amount of feeding work are not ideal, and even the phenomena of feeding midway and test failure occur. Most of the prior insect cages on the market are fixed, the size of the living space of the insects is fixed, and only one space is provided, so that the insect cages are not beneficial to observing the indexes of two tested insects competing in the same space, and are also not beneficial to observing the quantity change of insect populations in different spaces; simultaneously because of can not changing the inside volume of insect feeding cage and can't satisfy and raise the insect of different quantity, when the worm volume is less, the relatively great space of insect feeding cage makes the polypide comparatively disperse, inconvenient observation.
Therefore, a device which can separate and respectively feed and observe two kinds of insects at the same time and is beneficial to observing, measuring biological indexes of the insects and feeding the insects is needed to be designed.
This device convenient operation, easily observe, reduce intensity of labour's small-size insect biology index device, solved current insect feeding device and can not satisfy two kinds of examination worms and raise the problem of observing and can freely adjust different space sizes simultaneously.
Disclosure of Invention
In order to solve the technical problems, the invention adopts the following technical scheme:
do benefit to the device that surveys insect biological index and raise of observation, include:
a main insect-raising cabin body;
the lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect breeding bin main body, the bottom copper gauze is respectively connected with the side gauze lifting mechanism and the bottom end of the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze;
the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, the temperature and humidity sensing chip is mounted inside the insect breeding cabin body, and the display screen is mounted on the outer side of the insect breeding cabin body and is electrically connected with the temperature and humidity sensing chip;
and the storage battery is electrically connected with the temperature and humidity reading device.
Further, be provided with two cavities in the insect-raising storehouse main part, all be provided with in every cavity separate gauze elevating system, separate gauze elevating system including separating gauze lift spool and separating the gauze, separate gauze lift spool with it all is provided with two, two to separate gauze lift spool parallel arrangement, and two separate gauze lift spool difference swivelling joint in the top of the adjacent lateral wall of two cavities, two separate the gauze and connect respectively in two separate on the gauze lift spool.
Further, side gauze elevating system includes side gauze lift spool and side gauze, side gauze lift spool is provided with six groups, six groups side gauze lift spool is swivelling joint respectively in two on the cavity does not set up the inner wall of separating gauze elevating system, six groups side gauze lift spool all is connected with side gauze.
Further, still include host plant fixing device, host plant fixing device includes fixation clamp, flexible piece and the door that draws in storehouse, the door that draws in storehouse connect in raise insect storehouse main part top, be provided with the pore on the door that draws in storehouse, the fixation clamp connect in raise insect storehouse main part is kept away from on the outer wall of the door one side that draws in storehouse, flexible piece sliding connection in on the fixation clamp, flexible piece runs through to insert raise insect storehouse main part and extend to raise insect inside the storehouse main part.
Further, still include nutrition device, nutrition device includes nutrition liquid pipe, nutrition liquid pipe connect in raise insects storehouse main part lateral wall is last and be located draw storehouse door downside that contracts.
Further, still including putting the worm device, put the worm device including putting wormhole and plastics stopper, put the wormhole set up in on the lateral wall of insect feeding storehouse main part, the plastics stopper with put wormhole dismantlement and be connected.
Further, still put the device of getting including the fodder, the fodder is put the device of getting and is included fodder door and fodder dish, fodder door with insect feeding storehouse main part is articulated, the fodder dish sets up inside insect feeding storehouse main part.
Further, still include ventilative device, ventilative device including evenly set up in the bleeder vent at insect feeding storehouse main part top.
Further, the insect raising cabin main part adopts transparent glass material, insect raising cabin main part outer wall is provided with the scale mark.
The use method of the device which is beneficial to observing, measuring and raising the biological indexes of the insects comprises the following steps:
the method comprises the following steps: firstly, opening a telescopic bin door, putting the taken host plant into the insect breeding bin main body, unfolding the blades of the host plant, and properly adjusting the length of the telescopic sheet on the fixing clamp according to the size of the blades to enable the telescopic sheet to clamp and fix the blade tips of the host plant blades on the top of the insect breeding bin main body;
step two: the leaf stalks of the host plants extend out of the drawing and shrinking bin door, the drawing and shrinking bin door is put down, and the leaf stalks of the host plants penetrate through a pore passage in the middle of the lower edge of the drawing and shrinking bin door and are tightly attached by absorbent cotton;
step three: placing the leafstalk of the host plant into a nutrient solution tube, and pouring the prepared nutrient solution into the nutrient solution tube;
step four: opening a feed bin door of the feed placing and taking device, placing the prepared feed in a feed disc, placing the feed on copper yarns at the bottom of the insect breeding bin main body from the feed disc, and closing the feed bin door after all the feed is placed;
step five: punching an insect opening device, pulling up the plastic plug on the insect placing hole, adjusting the pipe orifice of the insect suction pipe to enable the pipe orifice of the insect suction pipe to correspond to the insect placing hole of the insect placing device, tapping the insect suction pipe to drive the insects in the insect suction pipe to the insect breeding device, and quickly plugging the plastic plug after all tested insects are placed in the device;
step six: fixing the gauze in a set space, so that the tested insects can conveniently stay on the leaves as soon as possible, and adjusting the scale of the gauze according to the requirement of the test when the tested insects are stable on the host plant;
step seven: if two kinds of test insects are bred and observed, the separation gauze separated in the middle of the insect breeding cabin main body is controlled to be lifted, and the separation gauze and other gauzes are adjusted to appropriate scales;
step eight: observing the temperature and humidity values of the hygrothermograph;
step nine: adding a proper amount of nutrient solution according to the reduced amount of the nutrient solution in the nutrient solution tube;
step ten: and starting a new round of test insect feeding after the steps are completed.
The invention has the beneficial effects that:
the device which is beneficial to observing, measuring and feeding the biological indexes of the insects and is provided by the invention has the advantages of simple and practical structure and high repeatability; the lifting of each side wall gauze and the bottom copper gauze in the device is controlled by a motor to change the size of a space, and the separation of the gauzes can also separate the space into two parts by adjusting the lifting height, so that the feeding and the observation of two types of test insects can be realized simultaneously. If the middle gauze is lifted to the top of the insect feeder, the insect feeding chamber becomes an independent space, the lifting gauze is fixed on a specified scale line, and the two insects are placed in the fixed space size, so that the indexes of the biological characteristics of the two insects and the competition relationship of the insects in different spaces can be observed conveniently; the nutrient solution pipe positioned on the outer wall is convenient for adding host plant nutrient solution, so that the freshness of the leaves is maintained, the replacement of the leaves is reduced, and the operation is simple and convenient; the temperature and humidity reading device can observe the temperature and the humidity in the test insect living space at any time, can be properly adjusted according to the life habits of the test insects, and can also know indexes such as the growth and development periods of the test insects under different temperature and humidity conditions.
Drawings
FIG. 1 is a schematic view of the device for observing, measuring and raising biological indicators of insects according to the present invention.
FIG. 2 is a schematic view of a nutrition liquid tube of the device for observing, measuring and raising biological indicators of insects according to the present invention.
FIG. 3 is a schematic view of the combination of the nutrient solution tube and the retractable door of the device for observing, measuring and raising biological indicators of insects according to the present invention.
FIG. 4 is a schematic diagram of the lifting switch of the device for observing, measuring and raising biological indicators of insects according to the present invention.
FIG. 5 is a schematic diagram of a humidity-temperature meter for observing, measuring and raising biological indicators of insects according to the present invention.
FIG. 6 is a schematic view of the rolled up state of the separation gauze lifting reel of the device for observing, measuring and raising biological indexes of insects.
FIG. 7 is a schematic view of the device for observing, measuring and raising biological indicators of insects according to the present invention, showing the state of the separated gauze lifting reel partially laid down.
FIG. 8 is a schematic view of the fixing clip of the device for observing, measuring and raising biological indicators of insects according to the present invention.
Fig. 9 is a schematic diagram of the survival rate of bemisia tabaci with different numbers in different space sizes in one day in the third example.
Fig. 10 is a schematic diagram of the survival rate of bemisia tabaci in different numbers in different space sizes within three days in example three.
Fig. 11 is a schematic diagram of the survival rate of bemisia tabaci with different numbers in different space sizes within seven days in the third embodiment.
Wherein, in the figure:
1-insect breeding cabin main body; 2-nutrient solution tube; 3-fixing the clamp; 4-a retractable bin gate; 5-a pore channel; 6-insect placing holes; 7-feed bin gate; 8-side screen yarn; 9-a lifting switch; 10-side screen lifting reel; 11-a display screen; 12-air holes; 13-separation gauze lifting reel, 14-separation gauze and 15-expansion sheet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 11 in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
The embodiment of the invention provides a device beneficial to observing, measuring and raising biological indexes of insects in combination with the embodiments of fig. 1-8, which comprises an insect raising cabin body 1, a lifting gauze device, a temperature and humidity reading device and a storage battery, wherein the lifting gauze device, the temperature and humidity reading device and the storage battery are connected to the insect raising cabin body 1, and the storage battery is electrically connected with the temperature and humidity reading device.
The lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and a bottom copper gauze, the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect breeding bin main body 1, the bottom copper gauze is respectively connected with the side gauze lifting mechanism and the bottom of the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze.
Be provided with two cavities in the insect-raising storehouse main part, all be provided with in every cavity separate gauze elevating system, separate gauze elevating system including separating gauze lift spool 13 and separating gauze 14, separate gauze lift spool 13 with separate gauze 14 and all be provided with two, two separate gauze lift spool 13 parallel arrangement, and two separate gauze lift spool 13 difference swivelling joint in the top of the adjacent lateral wall of two cavities, two separate gauze 14 connect respectively in two separate on the gauze lift spool 13.
Side gauze elevating system includes side gauze lift spool 10 and side gauze 8, side gauze 8 lift spool 10 is provided with six groups, six groups side gauze 8 lift spool 10 respectively swivelling joint in two the cavity does not set up the inner wall top of separating gauze elevating system, six groups side gauze 8 lift spool 10 all is connected with side gauze 8, makes it drive and separates gauze lift spool 13 and rotate, realizes separating the lift of gauze 14.
In the embodiment, the side gauze 8 and the separation gauze 14 both adopt 150-mesh gauze, so that small insects can not drill out, and the safety and the reliability are realized; the bottom copper yarn adopts 150-mesh copper yarn, is firm and reliable, and can hold up feed and a feed tray for feeding insects.
As optimization, in this embodiment the side gauze lifting reel 10 and the separation gauze lifting reel 13 adopt motor drive's mode, are connected each motor with the battery electricity respectively, and the battery provides the electric energy for each motor, and each motor all is provided with the lifting switch 9 of control motor operation, lifting switch 9 connect in the insect feeding storehouse main part 1 outside and with corresponding motor electricity be connected, improve the safety in utilization performance of motor.
Furthermore, the lifting switch 9 is made of waterproof materials, so that the failure of the lifting switch 9 caused by a field humid environment is avoided.
In other embodiments, the side screen lifting reel 10 and the partition screen lifting reel 13 are driven to rotate manually.
The temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen 11, the temperature and humidity sensing chip is mounted inside the insect breeding cabin main body 1, an insulating sheet is arranged on the temperature and humidity sensing chip, the influence of potential hazards such as electric leakage on insect testing in the insect breeding cabin main body is avoided, and the display screen 11 is mounted on the outer side of the insect breeding cabin main body 1 and is electrically connected with the temperature and humidity sensing chip; the setting up of display screen makes can direct display degree on the screen, conveniently reads the temperature and the humidity in the insect feeding storehouse main part more, observes the temperature and the air humidity condition in the examination worm life space at any time to can carry out appropriate physical adjustment according to examination worm life habit and experimental needs.
Furthermore, the device beneficial to observing, measuring and feeding biological indexes of insects further comprises a host plant fixing device and a nutrition device, wherein the host plant fixing device comprises a fixing clamp 3, a telescopic sheet 15 and a pull-in bin door 4, the pull-in bin door 4 is connected to the top end of the insect feeding bin main body 1, a hole passage 5 is formed in the pull-in bin door 4, the fixing clamp 3 is connected to the outer wall of the insect feeding bin main body 1 on one side far away from the pull-in bin door 4, the telescopic sheet 15 is connected to the fixing clamp 3 in a sliding mode, and the telescopic sheet 15 penetrates through the insect feeding bin main body 1 and extends into the insect feeding bin main body 1; the nutrition device comprises a nutrition liquid pipe 2, and the nutrition liquid pipe 2 is connected to the side wall of the insect feeding cabin body 1 and is positioned at the lower side of the pull-and-shrink cabin door 4; the petiole of the host plant passes through the pore passage and extends into the nutrient solution pipe, so that the soilless culture of the host plant is realized.
In the embodiment, two groups of host plant fixing devices are arranged and are respectively positioned in two spaces formed by separation of the separation gauze, the telescopic pieces 15 on the fixing clamps can stretch into the insect breeding chamber and are used for fixing the blade tips of the host plants, the maximum length of each telescopic piece 15 is 15cm, and the telescopic pieces 15 can be stretched and fixed according to the sizes of the host plant blades, so that the host plant blades are flatly laid on the top of the insect breeding device, the fixation of the host plant blades with various sizes is met, and the insect testing, feeding and oviposition are facilitated; the nutrient solution tube adopts a semi-cylindrical test tube which has the diameter of 20mm and is made of transparent plastic, liquid volume scales are arranged on the semi-cylindrical test tube, the maximum scales are 10ml, the amount of prepared nutrient solution is better controlled by setting the scales, the amount of prepared nutrient solution can be determined according to the scales on the nutrient solution tube, and the waste is reduced; the hole channel in the middle position of the lower edge of the shrinking cabin door right above the nutrient solution pipe is semicircular, the diameter of the hole channel is 10mm, enough space is reserved for the leaf stalk of the host plant, the leaf stalk of the host plant is not damaged, the survival time of the host plant is prolonged, and the fixation of the leaf stalk of the host plant can be carried out by matching absorbent cotton.
The insect feeding device comprises an insect feeding hole 6 and a plastic plug, wherein the insect feeding hole 6 is formed in the side wall of the insect feeding bin main body 1, and the plastic plug is detachably connected with the insect feeding hole 6; in this embodiment, the diameter of putting the wormhole is 20mm, also has the hole with the same size of putting the wormhole on the gauze that corresponds with putting the wormhole, has the rubber circle around the hole, makes the sealing performance of plastic plug better, avoids the examination worm to escape.
Further, the insect feeding device comprises a feed placing and taking device, wherein the feed placing and taking device comprises a feed bin door 7 and a feed plate, the feed bin door 7 is hinged with the insect feeding bin main body 1, and the feed plate is arranged inside the insect feeding bin main body 1; the feed bin door 7 is 10cm long and 3cm high in the embodiment, the handle is arranged in the middle of the feed bin door 7, the feed taking and placing device can meet the requirements of different test insects on different feeds, artificial feeds can be placed in addition to natural feeds capable of placing host plants, and the most suitable feed can be provided for the test insects.
As the optimization, still include ventilative device, ventilative device including evenly set up in the bleeder vent 12 at 1 top in insect feeding storehouse main part, in this embodiment the diameter of bleeder vent is 1mm, is provided with four rows of circular shape bleeder vents 12 altogether, and every row includes 10 circular shape bleeder vents 12, and every row of bleeder vent interval is 5cm, and every row of bleeder vent interval is 3cm, increases the taking a breath in the insect feeding storehouse main part through setting up of a plurality of bleeder vents.
Insect feeding storehouse main part 1 adopts the transparent glass material to make, insect feeding storehouse main part 1 outer wall is provided with the scale mark, and the setting of scale mark can more definitely control the life space that highly changes the examination worm of the lift of gauze to satisfy the observation to examination worm competitive relation in the different spaces.
The device which is beneficial to observing, measuring and feeding the biological indexes of the insects and is provided by the invention has the advantages of simple and practical structure and high repeatability; the lifting of each side wall gauze and the bottom copper gauze in the device is controlled by a motor to change the size of a space, and the separation of the gauzes can also separate the space into two parts by adjusting the lifting height, so that the feeding and the observation of two types of test insects can be realized simultaneously. If the middle gauze is lifted to the top of the insect feeder, the insect feeding chamber becomes an independent space, the lifting gauze is fixed on a specified scale line, and the two insects are placed in the fixed space size, so that the indexes of the biological characteristics of the two insects and the competition relationship of the insects in different spaces can be observed conveniently; the nutrient solution pipe positioned on the outer wall is convenient for adding host plant nutrient solution, so that the freshness of the leaves is maintained, the replacement of the leaves is reduced, and the operation is simple and convenient; the temperature and humidity reading device can observe the temperature and the humidity in the test insect living space at any time, can be properly adjusted according to the life habits of the test insects, and can also know indexes such as the growth and development periods of the test insects under different temperature and humidity conditions.
Example two
The use method of the device which is beneficial to observing, measuring and raising the biological indexes of the insects comprises the following steps:
the method comprises the following steps: firstly, opening a telescopic bin door, putting the taken host plant into the insect breeding bin main body, unfolding the blades of the host plant, and properly adjusting the length of the telescopic sheet on the fixing clamp according to the size of the blades to enable the telescopic sheet to clamp and fix the blade tips of the host plant blades on the top of the insect breeding bin main body;
step two: the leaf stalks of the host plants extend out of the drawing and shrinking bin door, the drawing and shrinking bin door is put down, and the leaf stalks of the host plants penetrate through a pore passage in the middle of the lower edge of the drawing and shrinking bin door and are tightly attached by absorbent cotton;
step three: placing the leafstalk of the host plant into a nutrient solution tube, and pouring the prepared nutrient solution into the nutrient solution tube;
step four: pulling open the feed bin door of the feed placing and taking device, placing the prepared feed in a feed tray, placing the feed tray on the copper yarn at the bottom of the insect feeding bin main body, and closing the feed bin door after all the feed trays are placed;
step five: punching an insect opening device, plugging up the plastic plug on the insect placing hole, adjusting the pipe orifice of the insect suction pipe to enable the pipe orifice of the insect suction pipe to correspond to the insect placing hole of the insect placing device, tapping the insect suction pipe to drive insects in the insect suction pipe to the insect breeding device, and quickly plugging the plastic plug after all tested insects are placed in the device;
step six: fixing the gauze in a set space, so that the tested insects can conveniently stay on the leaves as soon as possible, and adjusting the scale of the gauze according to the requirement of the test when the tested insects are stable on the host plant;
step seven: if two kinds of test insects are bred and observed, the separation gauze separated in the middle of the insect breeding cabin main body is controlled to be lifted, and the separation gauze and other gauzes are adjusted to appropriate scales;
step eight: observing the temperature and humidity values of the hygrothermograph;
step nine: adding a proper amount of nutrient solution according to the reduced amount of the nutrient solution in the nutrient solution tube;
step ten: and after the steps are finished, starting a new round of test insect feeding.
EXAMPLE III
The present embodiment is tested by using a device which is beneficial to observing, measuring biological indexes of insects and raising insects.
1. Selecting a test insect source and performing test operation:
in the experiment, sample plots planted with different crops are selected for observation feeding experiments, and a device which is favorable for observing and measuring biological indexes of insects and feeding, a self-sealing bag and an insect cage are adopted for observation feeding.
(1) Comparison of survival time for different numbers of insects raised on different host plants
TABLE 1 insects on different host plants are reared in different numbers for survival times
Note: the survival time values in the table are mean ± sem; the different lower case letters in the same row indicate that the same insect under different host plants survived significantly different times under different devices. (Tukey HSD test, P < 0.05)
The survival time of the bemisia tabaci on different host plants in different numbers is shown in table 1, and the bemisia tabaci was collected from tomatoes, the bemisia tabaci collected from cucumbers, and the bemisia tabaci collected from eggplants in the same number of 20. 20 tobacco whiteflies collected from tomatoes are bred and observed by the invention, the survival time is 19.67d, the self-sealing bag is 3.67d, and the insect rearing cage is 9d (F) 2,8 =380.2624, p = 0.0001). The survival time of 20 tobacco whiteflies collected from cucumbers and bred and observed by the method is 18.67d, the self-sealing bag is 3.33d, and the insect cage is 9.33d (F) 2,8 =187.4160, p = 0.0001). The survival time of 20 tobacco whiteflies collected from eggplants is 19.67d, the self-sealing bag is 4d, and the insect cage is 9.67d (F) 2,8 =313.4194, p = 0.0001). The survival time of the tomato after 40 heads of bemisia tabaci are collected until the tomato is bred for 17.67d, the self-sealing bag is 2.67d, and the insect cage is 6.00d (F) 2,8 =145.3110, p = 0.0001). The survival time of the cucumber after collecting 40 bemisia tabaci is 16.67d, the self-sealing bag is 2.33d and the insect cage is 5.67d (F) 2,8 =304.7616, p = 0.0001). Collecting 40 tobacco whiteflies from eggplants to feeding of the inventionThe survival time is 17.33d, the self-sealing bag is 2.00d, and the insect cage is 5.33d (F) 2,8 =366.6442, p = 0.0001). The survival time from the collection of 60 bemisia tabaci on tomatoes to the feeding of the invention is 15.67d, the self-sealing bag is 1.67d, and the insect cage is 2.33d (F) 2,8 =722.2215, p = 0.0001). The survival time of the cucumber after collecting 60 Bemisia tabaci is 15.33d, the self-sealing bag is 1.33d, and the insect cage is 2.67d (F) 2,8 =309.4408, p = 0.0001). The survival time of the eggplant after 60 Bemisia tabaci are collected until the eggplant is bred for 15.00d, the self-sealing bag is 2.00d, and the insect cage is 2.33d (F) 2,8 =288.9082, p = 0.0001). The survival time of the tomato after 80 heads of bemisia tabaci are collected until the tomato is bred for 11.67d, the self-sealing bag is 0.667d, and the insect cage is 1.33d (F) 2,8 =418.9303, p = 0.0001). The survival time of the cucumber after 80 heads of bemisia tabaci are collected until the cucumber is bred for 12.33d, the self-sealing bag is 0.333d, and the insect cage is 1.00d (F) 2,8 =146.1670, p = 0.0001). The survival time of the invention from the collection of 80 bemisia tabaci from eggplants to the breeding of the invention is 12.67d, the self-sealing bag is 0.667d, and the insect cage is 1.33d (F) 2,8 =211.4084, p = 0.0001). It can be seen from the table that the survival time of the insects collected from different plants in the self-sealing bag and the insect cage of the invention is obviously different, the survival time in the breeding of the insects of the invention is greatly prolonged compared with the survival time in the self-sealing bag and the insect cage of the invention, and the survival time of the insects bred of the invention is greatly increased along with the reduction of the number of the bred insects.
Note: the F value is the statistic of the F test, namely the ratio of the sum of squared deviations between groups and in groups to the degree of freedom, the significance is the significance level corresponding to the F statistic, 0.001 indicates that the original hypothesis is rejected, namely the significant difference exists between different levels of single factors, F (the degree of freedom between groups and the degree of freedom in groups) = specific numerical value, in the analysis of variance of the single factors, the degree of freedom between groups is r-1, the degree of freedom in groups is n-r, wherein r is the grouping level number of the factors, and n is the total number of samples. The P value is also called to test whether the P value is the strength of the original hypothesis, the principle of the P value is that the total hypothesis is the original hypothesis, a sample is extracted, under the original hypothesis, the probability value of the sample is the probability value of the small probability event rejecting the original hypothesis according to the principle that the small probability event is unlikely to occur in one experiment, the smaller the value is, the more unlikely the original hypothesis is, and the significance level of the original hypothesis can be rejected.
(2) Comparison of survival rates of different quantities of Bemisia tabaci in different spatial dimensions
In this example, the survival rates of different numbers of bemisia tabaci were observed when the bemisia tabaci was fed in different spaces on the same day, the survival rate of different numbers of bemisia tabaci in different space sizes (1 day) was observed on the first day as shown in fig. 9, and the space of the device was 300cm 3 The survival rate of 20 tobacco whiteflies bred is 86.7 percent, the survival rate of 40 tobacco whiteflies bred is 68.3 percent, and the survival rate of 60 tobacco whiteflies bred is 51.1 percent (F) 2,8 =238.9252, P = 0.0285). When the space of the device is 450cm 3 In the meantime, the survival rate of 20 heads of the tobacco whiteflies bred is 93.3 percent, the survival rate of 40 heads of the tobacco whiteflies bred is 78.3 percent, and the survival rate of 60 heads of the tobacco whiteflies bred is 62.8 percent (F) 2,8 =94.7709,p = 0.0285). When the space of the device is 600cm 3 In the meantime, the survival rate of 20-head tobacco whitefly fed is 96.7%, the survival rate of 40-head tobacco whitefly fed is 88.3%, and the survival rate of 60-head tobacco fed is 75.0% (F) 2,8 =21.8655, p = 0.0020). On the third day, as shown in FIG. 10, the survival rate of bemisia tabaci in different sizes of space (3 days) was determined when the space of the device was 300cm 3 In this case, the survival rate of 20 heads of the tobacco whitefly fed is 73.3%, the survival rate of 40 heads of the tobacco whitefly fed is 57.5%, and the survival rate of 60 heads of the tobacco whitefly fed is 42.2% (F) 2,8 =1.2634, p = 0.3838). When the space of the device is 450cm 3 In this case, the survival rate of 20 heads of the tobacco whiteflies bred was 90.0%, the survival rate of 40 heads bred was 66.7%, and the survival rate of 60 heads bred was 52.2% (F) 2,8 =113.2979, P = 0.0001). When the space of the device is 600cm 3 In this case, the survival rate of 20 heads of the tobacco whitefly fed is 93.3%, the survival rate of 40 heads of the tobacco whitefly fed is 81.7%, and the survival rate of 60 heads of the tobacco whitefly fed is 64.4% (F) 2,8 =40.1385, p = 0.0004). On day seven, as shown in FIG. 11, the survival rate of bemisia tabaci in different space sizes (7 days) was determined when the space of the device was 300cm 3 The survival rate of 20 tobacco whiteflies bred is 65.0 percent, the survival rate of 40 tobacco whiteflies bred is 45.0 percent, and the survival rate of 60 tobacco whiteflies bred is 31.1 percent (F) 2,8 =14.0498, p = 0.0001). When the space of the device is 450cm 3 When the temperature of the water is higher than the set temperature,the survival rate of 20 tobacco whiteflies bred is 80.0 percent, the survival rate of 40 tobacco whiteflies bred is 60.0 percent, and the survival rate of 60 tobacco whiteflies bred is 42.2 percent (F) 2,8 =113.0117, P = 0.0003). When the space of the device is 600cm 3 In the meantime, the survival rate of 20 heads of the tobacco whiteflies bred is 83.3 percent, the survival rate of 40 heads of the tobacco whiteflies bred is 70.8 percent, and the survival rate of 60 heads of the tobacco whiteflies bred is 51.1 percent (F) 2,8 =102.0596, p = 0.0001). It can be seen from the figure that the survival rates of different numbers of insects in different spaces of the invention are obviously different, the survival rate of the insects raised and observed in the invention is reduced along with the increase of the insect quantity in the unchanged space, and the survival rate of the insects in the invention is improved more obviously along with the increase of the living space of the insects.
In the present embodiment, the numerical values in the graphs of fig. 9, 10, and 11 are the mean value ± sem; the different lower case letters on the graph indicate that the survival rate of different numbers of bemisia tabaci collected on the same host plant was significantly different at different spatial sizes on the same day (Tukey HSD test, P < 0.05).
The experiment of this embodiment shows, do benefit to the observation survey insect biological index and raise the device and have very obvious advantage in specific experiment and assurance insect survival rate, raise different worm volume survival rates in different spaces different, so can guarantee the survival rate of insect through the most suitable space size of worm volume regulation of raising, utilize valve bag and insect feeding cage to raise observation insect, the survival rate of insect is less than the survival rate that adopts and does benefit to observation survey insect biological index and raising the device far away.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. Do benefit to device that survey insect biological index and raise of observation, its characterized in that includes:
a main insect feeding cabin body;
the lifting gauze device comprises a side gauze lifting mechanism, a separation gauze lifting mechanism and bottom copper gauze, wherein the side gauze lifting mechanism and the separation gauze lifting mechanism are connected to the insect breeding bin main body, the bottom copper gauze is respectively connected with the side gauze lifting mechanism and the bottom end of the separation gauze lifting mechanism, the side gauze lifting mechanism is used for adjusting the height of the side gauze, and the separation gauze lifting mechanism is used for adjusting the height of the separation gauze;
the temperature and humidity reading device comprises a temperature and humidity sensing chip and a display screen, the temperature and humidity sensing chip is mounted inside the insect breeding cabin body, and the display screen is mounted on the outer side of the insect breeding cabin body and is electrically connected with the temperature and humidity sensing chip;
and the storage battery is electrically connected with the temperature and humidity reading device.
2. The device for facilitating observation, determination and feeding of biological indexes of insects according to claim 1, wherein two chambers are arranged in the insect feeding chamber main body, the separation gauze lifting mechanism is arranged in each chamber, the separation gauze lifting mechanism comprises a separation gauze lifting scroll and a separation gauze, the separation gauze lifting scroll and the separation gauze are arranged in two, the two separation gauze lifting scrolls are arranged in parallel, the two separation gauze lifting scrolls are respectively and rotatably connected to the top ends of the adjacent side walls of the two chambers, and the two separation gauzes are respectively connected to the two separation gauze lifting scrolls.
3. The device for facilitating observation, determination and feeding of biological indicators of insects according to claim 2, wherein the side gauze lifting mechanism comprises side gauze lifting reels and side gauze, the side gauze lifting reels are provided with six groups, the six groups of side gauze lifting reels are respectively connected on the inner walls of the two chambers without the separation gauze lifting mechanism in a rotating manner, and the six groups of side gauze lifting reels are connected with the side gauze.
4. The device for facilitating observation, measurement and feeding of insect biological indexes according to claim 1, further comprising a host plant fixing device, wherein the host plant fixing device comprises a fixing clip, a telescopic piece and a telescopic door, the telescopic door is connected to the top end of the insect feeding chamber main body, a hole is formed in the telescopic door, the fixing clip is connected to the outer wall of one side, away from the telescopic door, of the insect feeding chamber main body, the telescopic piece is connected to the fixing clip in a sliding mode, penetrates through the insect feeding chamber main body and extends to the inside of the insect feeding chamber main body.
5. The apparatus for facilitating observation, measurement and feeding of biological indicators of insects as claimed in claim 4, further comprising a nutrition device, said nutrition device comprising a nutrition liquid tube, said nutrition liquid tube is connected to the side wall of said insect feeding chamber body and located under the door of said stretching chamber.
6. The device for facilitating observation, determination and feeding of insect biological indexes according to claim 1, further comprising an insect placing device, wherein the insect placing device comprises an insect placing hole and a plastic plug, the insect placing hole is formed in a side wall of the insect feeding chamber main body, and the plastic plug is detachably connected with the insect placing hole.
7. The device for facilitating observation, measurement and feeding of insect biological indexes according to claim 1, further comprising a feed placing and taking device, wherein the feed placing and taking device comprises a feed bin door and a feed disc, the feed bin door is hinged with the insect feeding bin main body, and the feed disc is arranged inside the insect feeding bin main body.
8. The device for facilitating observation, measurement and feeding of biological indicators of insects as claimed in claim 1, further comprising a ventilation device, wherein the ventilation device comprises ventilation holes uniformly arranged on the top of the main body of the insect feeding chamber.
9. The device for facilitating observation, measurement and feeding of insect biological indexes according to claim 1, wherein the insect feeding chamber body is made of transparent glass material, and the outer wall of the insect feeding chamber body is provided with scale marks.
10. The use method of the device which is beneficial to observing, measuring and raising the biological indexes of the insects comprises the following steps:
the method comprises the following steps: firstly, opening a telescopic bin door, putting the taken host plant into the insect breeding bin main body, unfolding the blades of the host plant, and properly adjusting the length of the telescopic sheet on the fixing clamp according to the size of the blades to enable the telescopic sheet to clamp and fix the blade tips of the host plant blades on the top of the insect breeding bin main body;
step two: stretching the leafstalk of the host plant out of the pull-shrink bin door, putting down the pull-shrink bin door, and enabling the leafstalk of the host plant to pass through a pore passage in the middle of the lower edge of the pull-shrink bin door and be tightly attached by absorbent cotton;
step three: placing the leafstalk of the host plant into a nutrient solution tube, and pouring the prepared nutrient solution into the nutrient solution tube;
step four: pulling open the feed bin door of the feed placing and taking device, placing the prepared feed in a feed tray, placing the feed tray on the copper yarn at the bottom of the insect feeding bin main body, and closing the feed bin door after all the feed trays are placed;
step five: punching an insect opening device, plugging up the plastic plug on the insect placing hole, adjusting the pipe orifice of the insect suction pipe to enable the pipe orifice of the insect suction pipe to correspond to the insect placing hole of the insect placing device, tapping the insect suction pipe to drive insects in the insect suction pipe to the insect breeding device, and quickly plugging the plastic plug after all tested insects are placed in the device;
step six: fixing the gauze in a set space, so that the tested insects can conveniently stay on the leaves as soon as possible, and adjusting the scale of the gauze according to the requirement of the test when the tested insects are stable on the host plant;
step seven: if two kinds of test insects are bred and observed, the separation gauze separated in the middle of the insect breeding cabin main body is controlled to be lifted, and the separation gauze and other gauzes are adjusted to appropriate scales;
step eight: observing the temperature and humidity values of the hygrothermograph;
step nine: adding a proper amount of nutrient solution according to the reduced amount of the nutrient solution in the nutrient solution tube;
step ten: and after the steps are finished, starting a new round of test insect feeding.
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