CN115279893A - 动物细胞的培养方法 - Google Patents

动物细胞的培养方法 Download PDF

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Publication number
CN115279893A
CN115279893A CN202180021549.7A CN202180021549A CN115279893A CN 115279893 A CN115279893 A CN 115279893A CN 202180021549 A CN202180021549 A CN 202180021549A CN 115279893 A CN115279893 A CN 115279893A
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China
Prior art keywords
medium
culture
active ingredient
methyl
cysteine
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Pending
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CN202180021549.7A
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English (en)
Chinese (zh)
Inventor
佐藤诚一
绯田由希
远藤裕太
松叶惠奈
辻千寻
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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Publication of CN115279893A publication Critical patent/CN115279893A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/04—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
    • C07D277/06—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202180021549.7A 2020-03-16 2021-03-15 动物细胞的培养方法 Pending CN115279893A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020045363 2020-03-16
JP2020-045363 2020-03-16
PCT/JP2021/010343 WO2021187411A1 (ja) 2020-03-16 2021-03-15 動物細胞の培養方法

Publications (1)

Publication Number Publication Date
CN115279893A true CN115279893A (zh) 2022-11-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180021549.7A Pending CN115279893A (zh) 2020-03-16 2021-03-15 动物细胞的培养方法

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Country Link
US (1) US20230044979A1 (https=)
EP (1) EP4123013A4 (https=)
JP (1) JPWO2021187411A1 (https=)
KR (1) KR20220154694A (https=)
CN (1) CN115279893A (https=)
WO (1) WO2021187411A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117165511A (zh) * 2023-08-15 2023-12-05 上海迈邦生物科技有限公司 一种细胞培养用的中性补料培养基及其制备方法和应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202540401A (zh) * 2023-11-24 2025-10-16 法商賽諾菲公司 合成四氫噻唑在細胞培養進料中作為半胱胺酸之遞送方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009227660A (ja) * 2008-02-28 2009-10-08 Ajinomoto Co Inc システイン誘導体
WO2009154014A1 (ja) * 2008-06-19 2009-12-23 日本理化学薬品株式会社 外用組成物
JP2012239432A (ja) * 2011-05-20 2012-12-10 Ajinomoto Co Inc 動物細胞培養用培地および該培地を用いた物質の製造方法
WO2017188082A1 (ja) * 2016-04-27 2017-11-02 味の素株式会社 培地添加剤

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5439849B2 (https=) 1974-03-08 1979-11-30
US20060110826A1 (en) * 2002-07-30 2006-05-25 Massachusetts Institute Of Technology Methods for perfusion and plating of primary hepatocytes and a medium therefore
KR101496795B1 (ko) * 2010-05-28 2015-03-02 아지노모토 가부시키가이샤 시스테인 유도체
EP2786741B1 (en) * 2011-11-30 2018-12-26 Ajinomoto Co., Inc. Whitening cosmetic
JP7057558B2 (ja) 2015-11-25 2022-04-20 味の素株式会社 フィブロイン様タンパク質の製造法
CN108884458A (zh) 2016-03-31 2018-11-23 味之素株式会社 丝心蛋白样蛋白质改造体及细胞培养方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009227660A (ja) * 2008-02-28 2009-10-08 Ajinomoto Co Inc システイン誘導体
WO2009154014A1 (ja) * 2008-06-19 2009-12-23 日本理化学薬品株式会社 外用組成物
JP2010001239A (ja) * 2008-06-19 2010-01-07 Nippon Rikagaku Yakuhin Kk 外用組成物
JP2012239432A (ja) * 2011-05-20 2012-12-10 Ajinomoto Co Inc 動物細胞培養用培地および該培地を用いた物質の製造方法
WO2017188082A1 (ja) * 2016-04-27 2017-11-02 味の素株式会社 培地添加剤

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KUSCHELEWSKI, J.ET AL.: "Antioxidant Effect of Thiazolidine Molecules in Cell Culture Media Improves Stability and Performance", BIOTECHNOLPROG, vol. 33, no. 3, 31 December 2017 (2017-12-31) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117165511A (zh) * 2023-08-15 2023-12-05 上海迈邦生物科技有限公司 一种细胞培养用的中性补料培养基及其制备方法和应用

Also Published As

Publication number Publication date
WO2021187411A1 (ja) 2021-09-23
JPWO2021187411A1 (https=) 2021-09-23
KR20220154694A (ko) 2022-11-22
US20230044979A1 (en) 2023-02-09
EP4123013A4 (en) 2024-08-21
EP4123013A1 (en) 2023-01-25

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Application publication date: 20221101