CN115279887A - 高活性nk细胞的处理方法 - Google Patents

高活性nk细胞的处理方法 Download PDF

Info

Publication number
CN115279887A
CN115279887A CN202180018428.7A CN202180018428A CN115279887A CN 115279887 A CN115279887 A CN 115279887A CN 202180018428 A CN202180018428 A CN 202180018428A CN 115279887 A CN115279887 A CN 115279887A
Authority
CN
China
Prior art keywords
cells
activity
hours
medium
frozen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180018428.7A
Other languages
English (en)
Chinese (zh)
Inventor
原田结
米满吉和
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gaia Biomedicine Inc
Original Assignee
Gaia Biomedicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gaia Biomedicine Inc filed Critical Gaia Biomedicine Inc
Publication of CN115279887A publication Critical patent/CN115279887A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/12—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Dispersion Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
CN202180018428.7A 2020-03-02 2021-03-02 高活性nk细胞的处理方法 Pending CN115279887A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020035297A JP7793284B2 (ja) 2020-03-02 2020-03-02 高活性ｎｋ細胞の処理方法
JP2020-035297 2020-03-02
PCT/JP2021/007863 WO2021177279A1 (ja) 2020-03-02 2021-03-02 高活性ｎｋ細胞の処理方法

Publications (1)

Publication Number Publication Date
CN115279887A true CN115279887A (zh) 2022-11-01

Family

ID=77614337

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180018428.7A Pending CN115279887A (zh) 2020-03-02 2021-03-02 高活性nk细胞的处理方法

Country Status (8)

Country Link
US (1) US20230109717A1 (https=)
EP (1) EP4151717A4 (https=)
JP (2) JP7793284B2 (https=)
KR (1) KR20220148233A (https=)
CN (1) CN115279887A (https=)
AU (1) AU2021230860A1 (https=)
TW (1) TW202140770A (https=)
WO (1) WO2021177279A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2023038037A1 (https=) * 2021-09-08 2023-03-16
TW202413411A (zh) 2022-08-30 2024-04-01 日商蓋亞生物製藥有限公司 單抗體(monobody)及NK細胞
KR20250161543A (ko) 2023-03-15 2025-11-17 가부시키가이샤 가이아바이오메디신 세포를 현탁하기 위한 액 및 그 이용
KR102940135B1 (ko) 2024-01-05 2026-03-16 인하대학교 산학협력단 고삼투 및 진동 자극에 노출된 배양보조세포를 통한 nk 세포의 효과적인 활성화 및 이의 용도
CN121294344A (zh) * 2024-06-20 2026-01-09 杭州百瑞竞康生物技术有限公司 Nk细胞无血清培养基

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103097520A (zh) * 2010-07-13 2013-05-08 人类起源公司 产生自然杀伤细胞的方法
CN109310710A (zh) * 2015-10-15 2019-02-05 细胞结构公司 自然杀伤细胞和ilc3细胞及其用途
CN109788752A (zh) * 2016-07-22 2019-05-21 组织测试技术有限公司 用糖脂增强细胞冷冻保存

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4385158B2 (ja) * 2000-12-04 2009-12-16 株式会社リンフォテック 細胞の保存液および該保存液を用いた細胞の保存方法
JP4947948B2 (ja) 2004-10-12 2012-06-06 ニプロ株式会社 細胞保存液
EP2468850A4 (en) 2009-08-19 2013-02-20 Takara Bio Inc CELL PRESERVATION PROCESS
WO2014083133A1 (en) * 2012-11-29 2014-06-05 Cellectis Sa Improved methods for producing mammalian pluripotent stem cell-derived endodermal cells
WO2016122014A1 (ko) * 2015-01-27 2016-08-04 한국생명공학연구원 자연살해세포의 대량생산 방법 및 상기 방법으로 수득된 자연살해세포의 항암제로서의 용도
SG11201909856XA (en) * 2017-05-04 2019-11-28 Mesoblast Int Sarl Mesenchymal lineage precursor or stem cells with enhanced immunosuppression
JP6647240B2 (ja) 2017-05-12 2020-02-14 米満　吉和 高活性ｎｋ細胞、およびその利用
JP2019024418A (ja) * 2017-07-31 2019-02-21 公立大学法人大阪府立大学 選択分離用培地
JP6543375B1 (ja) 2018-03-27 2019-07-10 株式会社ガイアバイオメディシン ケモカインレセプターと細胞接着分子を発現するｃｄ３陰性細胞の集団、およびその利用
CN114615886A (zh) * 2019-08-29 2022-06-10 得克萨斯大学体系董事会 细胞冷冻保存培养基

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103097520A (zh) * 2010-07-13 2013-05-08 人类起源公司 产生自然杀伤细胞的方法
CN109310710A (zh) * 2015-10-15 2019-02-05 细胞结构公司 自然杀伤细胞和ilc3细胞及其用途
CN109788752A (zh) * 2016-07-22 2019-05-21 组织测试技术有限公司 用糖脂增强细胞冷冻保存

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAN ZHAO等: "Endoplasmic reticulum stress inhibition is a valid therapeutic strategy in vitrifying oocytes", CRYOBIOLOGY, vol. 20, no. 1, pages 371 - 987 *

Also Published As

Publication number Publication date
EP4151717A4 (en) 2024-04-17
JP7793284B2 (ja) 2026-01-05
AU2021230860A1 (en) 2022-09-22
TW202140770A (zh) 2021-11-01
EP4151717A1 (en) 2023-03-22
WO2021177279A1 (ja) 2021-09-10
KR20220148233A (ko) 2022-11-04
JP2021136883A (ja) 2021-09-16
US20230109717A1 (en) 2023-04-13
JP2023126695A (ja) 2023-09-07

Similar Documents

Publication Publication Date Title
CN115279887A (zh) 高活性nk细胞的处理方法
Yuan et al. Cryopreservation of human mesenchymal stromal cells expressing TRAIL for human anti-cancer therapy
CN102597223B (zh) 生产天然杀伤细胞的方法
Zhang et al. Mesenchymal stem cells derived extracellular vesicles alleviate traumatic hemorrhagic shock induced hepatic injury via IL-10/PTPN22-mediated M2 Kupffer cell polarization
AU2018346818B2 (en) Expansion and use of expanded NK cell fractions
CN108220235A (zh) 一种体外活化扩增人自然杀伤（nk）细胞的方法及其专用培养体系
CN113557295A (zh) 哺乳动物细胞的保存液
WO2015076717A2 (en) Mscs in the treatment of cardiac disorders
Plantinga et al. Clinical grade production of wilms’ tumor-1 loaded cord blood-derived dendritic cells to prevent relapse in pediatric AML after cord blood transplantation
JP2024519222A (ja) ＮＫ細胞の凍結保存用組成物及びこれを含む凍結保存剤形｛ＮＫ ｃｅｌｌ ｃｒｙｏｐｒｅｓｅｒｖａｔｉｏｎ ｃｏｍｐｏｓｉｔｉｏｎ ａｎｄ ｃｒｙｏｐｒｅｓｅｒｖａｔｉｏｎ ｆｏｒｍｕｌａｔｉｏｎ ｃｏｍｐｒｉｓｉｎｇ ｔｈｅ ｓａｍｅ｝
CN118103492A (zh) 细胞的处理方法
HK40083554A (en) Method for treating highly active nk cells
CN107779432A (zh) 一种car‑t细胞制剂及低温保存介质
KR20250161543A (ko) 세포를 현탁하기 위한 액 및 그 이용
HK40084673A (en) Method for treating highly active nk cells
Córdoba-Espejo et al. GMP-compliant manufacturing of allogeneic peripheral blood CAR-NK cells for the treatment of acute myeloid leukemia
US20250255906A1 (en) Formulations and methods for cellular therapies involving chimeric antigen receptors
HK40106614A (zh) 细胞的处理方法
HK40108875A (en) Method for treating cells
US20220233676A1 (en) Formulations of activating antigen carriers
WO2026096743A1 (en) Methods for gamma delta t cells manufacturing
WO2024233960A2 (en) Compositions comprising and methods of using dendritic cell exosomes
HK40005831A (zh) 扩增自然杀手细胞部份的扩增方法及用途
LT6263B (lt) Dendritinių ląstelių (dl) gavimo iš periferinio kraujo, kaulų čiulpų arba aferezės produkto būdas ir jų panaudojimas

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40083554

Country of ref document: HK