CN115254834A - Magnetic particle cleaning device and cleaning method for immunoassay device - Google Patents
Magnetic particle cleaning device and cleaning method for immunoassay device Download PDFInfo
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- CN115254834A CN115254834A CN202210922246.9A CN202210922246A CN115254834A CN 115254834 A CN115254834 A CN 115254834A CN 202210922246 A CN202210922246 A CN 202210922246A CN 115254834 A CN115254834 A CN 115254834A
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- 239000006249 magnetic particle Substances 0.000 title claims abstract description 185
- 238000004140 cleaning Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000003018 immunoassay Methods 0.000 title claims abstract description 24
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 238000005406 washing Methods 0.000 claims description 9
- 230000000630 rising effect Effects 0.000 claims description 8
- 230000001788 irregular Effects 0.000 claims description 7
- 230000001900 immune effect Effects 0.000 claims 1
- 239000011859 microparticle Substances 0.000 claims 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 15
- 238000001514 detection method Methods 0.000 description 15
- 238000013459 approach Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- 239000012491 analyte Substances 0.000 description 8
- 239000010419 fine particle Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000003068 static effect Effects 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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Abstract
The invention aims to provide a magnetic particle cleaning device and a cleaning method for immunoassay equipment, which comprise a reaction container, a first magnetic block, a second magnetic block and a third magnetic block; the bottom of the reaction vessel is conical; the first magnetic block is arranged on the side surface of the first side wall, the second magnetic block is arranged on one side of the second side wall, and the third magnetic block is arranged on the bottom side of the reaction container; the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a broken line track; and the bottom of the reaction container is close to one side of the third magnetic block, the reaction container is driven by the moving mechanism to swing between the first magnetic block and the second magnetic block and ascend in a broken line track, the combined magnetic particles shuttle in a reaction liquid in the reaction container in a broken line manner, and the surfaces of the combined magnetic particles and the objects to be tested which are not combined with the magnetic particles and are mixed between the combined magnetic particles are cleaned in the process of shuttling in the reaction liquid.
Description
Technical Field
The invention relates to the technical field of immunoassay equipment, in particular to a magnetic particle cleaning device and a cleaning method for immunoassay equipment.
Background
The immunoassay device is used for detection and analysis techniques of analytes such as various antigens, haptens, antibodies, hormones and the like. The magnetic particle chemiluminescence immune analysis is a labeling immune determination technology for detecting trace substance to be detected, and the combined magnetic particle is a combination body of the magnetic particle and the substance to be detected after reaction. The object to be detected which is not combined with the magnetic particles in a reaction manner still affects the accuracy of the detection result in the subsequent detection, and therefore, the object to be detected which is not combined with the magnetic particles in a reaction manner is cleaned, specifically, the object to be detected which is attached to the surface of the combined magnetic particles or is sandwiched between the combined magnetic particles and is not combined with the magnetic particles in a reaction manner is cleaned, so that the object to be detected which is not combined with the magnetic particles in a reaction manner is cleaned, and the influence of the object to be detected which is not combined with the magnetic particles in a reaction manner on the detection accuracy is prevented.
In the existing combined magnetic particle cleaning device, after a certain amount of mixed liquid of an object to be detected and magnetic particles is added into a reaction container, the object to be detected and the magnetic particles react to form combined magnetic particles, the reaction container is moved to a magnet position, the magnet adsorbs and gathers the combined magnetic particles in the reaction container to one side of the inner wall of the container, the liquid in the reaction container is completely sucked through a waste suction needle, then, cleaning liquid is added after the reaction container leaves the magnet, the gathered combined magnetic particles are dispersed to a uniform mixing state, the reaction container is moved to the same or same side magnet position, the combined magnetic particles in the reaction container are adsorbed and gathered again, the liquid in the reaction container is completely sucked through the waste suction needle, and after the actions are repeated for multiple times, the combined magnetic particle cleaning is completed.
The cleaning device and the cleaning method are easy to clean incompletely, and especially, residual objects to be detected attached to the surfaces of the combined magnetic particles or clamped among the combined magnetic particles influence the subsequent detection precision; meanwhile, the processes of extracting waste liquid and filling cleaning liquid are adopted, so that a liquid path system of the system is complicated, and waste liquid and environment pollution are easily caused.
In view of the above, it is necessary to improve the conventional cleaning apparatus and cleaning method to solve the above technical problems.
Disclosure of Invention
In order to solve the above technical problems, an object of the present invention is to provide a magnetic particle cleaning apparatus and a cleaning method for an immunoassay device, which can clean the combined magnetic particles more thoroughly by moving and matching a plurality of magnetic blocks and a reaction container.
In order to achieve the first object, the present application provides a magnetic particle cleaning device for an immunoassay device, comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side surface of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the reaction vessel is driven by the moving mechanism to swing between the first magnetic block and the second magnetic block and ascend in a broken line track, and the reaction vessel stops ascending after ascending to the highest position.
Preferably, the reactor further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
and when the reaction container rises to the highest position, stopping rising, moving the reaction container to the third magnetic block, enabling the bottom of the reaction container to be close to one side of the third magnetic block, and enabling the combined magnetic particles to be gathered at the bottom of the reaction container.
In order to achieve the above first object, the present application further provides a magnetic particle cleaning device for an immunoassay device, comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side surface of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the first magnetic block and the second magnetic block are respectively close to the first side wall and the second side wall of the reaction container in a staggered mode and descend in a broken line track, and the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container.
Preferably, the reactor further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
when the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, the third magnetic block moves to the bottom of the reaction container, one side of the third magnetic block is close to the bottom of the reaction container, and combined magnetic particles are gathered at the bottom of the reaction container.
Preferably, the reaction vessel is provided with a micro-hole at the bottom and a pipette at the top.
Preferably, the first magnetic block, the second magnetic block and the third magnetic block are all rectangular, circular or irregular in shape, and are formed by splicing a plurality of magnetic blocks respectively.
In order to achieve the second object, the present application provides a magnetic particle cleaning method for an immunoassay device, comprising the steps of:
a reaction liquid containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
the reaction vessel swings between the first magnetic block and the second magnetic block which are relatively fixed and rises in a broken line track;
and when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container.
In order to achieve the second object, the present application also provides a magnetic particle cleaning method for an immunoassay device, comprising the steps of:
a reaction solution containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
the first magnetic block and the second magnetic block which are arranged on two sides of the relatively fixed reaction container are close to the side wall of the reaction container in a staggered mode and descend in a broken line track;
after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, the third magnetic block moves to the bottom of the reaction container, one side of the third magnetic block is close to the bottom of the reaction container, and combined magnetic particles are gathered at the bottom of the reaction container.
Preferably, the bottom of the reaction vessel is conical, the bottom of the reaction vessel is provided with a micro-hole, and the top of the reaction vessel is provided with a pipette.
Preferably, the first magnetic block, the second magnetic block and the third magnetic block are all rectangular, circular or irregular in shape, and the first magnetic block, the second magnetic block and the third magnetic block are formed by splicing a plurality of two magnetic blocks respectively.
Through the technical scheme, the technical effects are as follows:
(1) The reaction container swings between the first magnetic block and the second magnetic block under the driving of the moving mechanism and rises in a broken line track, specifically, when the reaction container swings to the first magnetic block, a first side wall of the reaction container is attached to or close to the first magnetic block, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall, when the reaction container swings to the second magnetic block, a second side wall of the reaction container is attached to or close to the second magnetic block, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall; in addition, the reaction container rises along a broken line track while swinging, so that the combined magnetic particles shuttle in a reaction liquid in the reaction container in a broken line manner, and in the process of shuttling in the reaction liquid, the surfaces of the combined magnetic particles and the objects to be detected which are not combined with the magnetic particles and are mixed among the combined magnetic particles are cleaned, so that the objects to be detected which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
(2) The first magnetic block and the second magnetic block are respectively close to the first side wall and the second side wall of the reaction container in a staggered manner and descend in a broken line track, and specifically, when the first magnetic block is attached to or close to the first outer wall of the reaction container, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall, and then the first magnetic block is far away from the reaction container in a broken line manner; when the second magnetic block is attached to or close to the second outer wall of the reaction vessel, the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall, and the second magnetic block is far away from the reaction vessel in a folding line; the first magnetic block and the second magnetic block approach the first side wall and the second side wall of the reaction container in a staggered manner according to the process and descend in a broken line track until the first magnetic block or the second magnetic block descends to the lowest position of the reaction container and approaches the reaction container; the first magnetic block and the second magnetic block are respectively close to the first side wall and the second side wall of the reaction container in a staggered manner and descend in a broken line track, so that the combined magnetic particles shuttle in a reaction liquid in the reaction container in a broken line manner, and in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container, so that the gathering, dispersing and reaggregating processes can occur, and the shuttle in a broken line manner is adopted, so that the shuttling stroke of the combined magnetic particles is greatly increased, the surfaces of the combined magnetic particles and objects to be detected which are mixed between the combined magnetic particles and are not combined with the magnetic particles are cleaned, and the subsequent detection precision is prevented from being influenced by the objects to be detected which are not combined with the magnetic particles.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic perspective view of the reaction vessel of the present invention in a position opposite to a magnetic block.
FIG. 2 is a schematic top view of the reactor vessel of the present invention relative to a magnet.
FIG. 3 is a schematic front view showing the relative positions of the magnetic blocks and the reaction vessel according to the present invention.
FIG. 4 is a schematic side view of the relative position of the magnetic block and the reaction vessel of the present invention.
FIG. 5 is a schematic diagram showing the movement locus of the reaction vessel of the present invention.
FIG. 6 is a schematic diagram of the moving track of the magnetic block of the present invention.
FIG. 7 is a schematic diagram of a magnetic block of the present invention.
FIG. 8 is a schematic diagram of the movement trace of the magnetic particles after the combination of the present invention.
FIG. 9 is a flow chart of the cleaning method of the present invention.
FIG. 10 is a flow chart of the cleaning method of the present invention.
Wherein, 1, a reaction vessel; 11. a first outer wall; 12. a second outer wall; 14. a pipettor; 14. splicing lines; 15. a micro-hole; 2. a first magnetic block; 3. a second magnetic block; 4. and a third magnetic block.
Detailed Description
The technical solutions of the present invention will be described in detail with reference to specific examples, which should be understood as illustrative only and not as limiting the scope of the invention, and various equivalent modifications of the invention will fall within the scope of the appended claims of the present application after reading the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Example 1
Fig. 1 to 5 show an embodiment of a magnetic particle washing apparatus for an immunoassay device according to the present invention.
As shown in fig. 1 to 5, the magnetic particle cleaning device for an immunoassay device includes a reaction vessel 1, a first magnetic block 2, a second magnetic block 3, and a third magnetic block 4; the bottom of the reaction vessel 1 is conical, and the reaction vessel 1 comprises a first side wall 12 and a second side wall 12; the first magnetic block 2 is arranged on the side surface of the first side wall 11, and the second magnetic block 3 is arranged on one side of the second side wall 12; the reaction container 1 swings between the first magnetic block 2 and the second magnetic block 3 under the drive of the moving mechanism and rises in a broken line track, and when the reaction container 1 rises to the highest position, the reaction container stops rising.
Specifically, referring to fig. 1 to 5, in order to thoroughly clean the object to be measured adsorbed on the surface of the combined magnetic particles or between the combined magnetic particles, at present, the combined magnetic particles are adsorbed or released to the cleaning solution by approaching and separating the same magnetic block from the reaction container, and the cleaning solution is pumped and filled for many times, so that the cleaning efficiency is low, the movement range of the combined magnetic particles in the cleaning solution is small, the combined magnetic particles are not thoroughly cleaned in the cleaning solution, and the object to be measured is also partially adsorbed on the surface of the combined magnetic particles or is sandwiched between the combined magnetic particles. In order to solve the technical problem, the reaction vessel 1 is driven by a moving mechanism to swing between the first magnetic block 2 and the second magnetic block 3 and ascend in a zigzag track, specifically, when the reaction vessel 1 swings to the first magnetic block 2, the first side wall 11 of the reaction vessel 1 is attached to or close to the first magnetic block 2, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and when the reaction vessel 1 swings to the second magnetic block 3, the second side wall 12 of the reaction vessel 1 is attached to or close to the second magnetic block 3, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12; in addition, the reaction container 1 rises along a broken-line track while swinging, so that the combined magnetic particles shuttle along a broken line in a reaction liquid in the reaction container, in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of two sides of the reaction container 1, the processes of gathering, dispersing and re-gathering can occur, the shuttling track refers to fig. 8, the shuttling stroke of the combined magnetic particles is greatly increased, and therefore the cleaning of the surfaces of the combined magnetic particles and the objects to be measured, which are not combined with the magnetic particles and are mixed between the combined magnetic particles, is realized, and the influence of the objects to be measured, which are not combined with the magnetic particles, on the subsequent detection precision is avoided.
It should be noted that, after the reaction vessel 1 rises to the highest position, the reaction vessel stops rising, where the highest position refers to that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1, and at this time, the combined magnetic particles are already adsorbed to the lowest part of the reaction vessel 1, and the combined magnetic particles can be taken out in an extruding or sucking manner; the moving mechanism in this embodiment drives the reaction vessel 1 to swing and rise in a zigzag manner, and since the specific structure of the moving mechanism is not the key point of the present invention, the moving mechanism may be designed conventionally, for example, by using a manipulator, a multi-degree-of-freedom guide rail, and other moving mechanisms, the specific structure of the moving mechanism is not described in detail herein, and the understanding of those skilled in the art is not affected.
Referring to fig. 8, as a preferred embodiment, a micro-hole 15 is disposed at the bottom of the reaction vessel 1, and a pipette 13 is disposed at the top of the reaction vessel 1. Specifically, the pipette tip of the pipette 13 is inserted into the inlet of the reaction vessel 1 and sealed so that the reaction solution does not flow out of the micro-holes of the reaction vessel 1, and when the bonded magnetic fine particles are adsorbed to the lowest conical tip by the washing device, the pipette 13 is controlled to discharge the lowest bonded magnetic fine particles in the reaction vessel 1 through the micro-holes, and the discharged bonded magnetic fine particles are measured.
Example 2
On the basis of the embodiment 1, the embodiment 2 further comprises a third magnetic block 4, wherein the third magnetic block 4 is arranged at the bottom side of the reaction vessel 1; when the reaction vessel 1 rises to the highest position, the reaction vessel 1 stops rising, the reaction vessel 1 moves and descends to the third magnetic block 4, the bottom of the reaction vessel 1 is close to one side of the third magnetic block 4, and the combined magnetic particles are gathered at the bottom of the reaction vessel 1, as shown in fig. 4 specifically.
Specifically, in embodiment 1, although the bonded magnetic particles are adsorbed to the lowermost part of the reaction vessel 1, since the first magnetic block 2 or the second magnetic block 3 is located on the side of the reaction vessel 1, the bonded magnetic particles may be aggregated to the side wall, and a small amount of the sample may be taken out when the bonded magnetic particles are pushed out or taken out, the bonded magnetic particles are completely and unbiased and aggregated to the lowermost part of the reaction vessel 1 by the third magnetic block 4 provided on the bottom side of the reaction vessel 1, and the sample may not be taken out when the bonded magnetic particles are pushed out or taken out.
As a preferred embodiment, the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 are all rectangular, circular, or irregular in shape, and the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 are respectively formed by splicing a plurality of magnetic blocks. Specifically, no matter the shape of the magnetic block is round or rectangular, the combined magnetic particles will be gathered and distributed on the corresponding inner wall of the magnetic block; referring to fig. 7, taking the splicing of two magnetic blocks as an example, the splicing lines are respectively located in the middle of the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4. Specifically, for the spliced magnetic block, the magnetic field at the splicing line 14 is strongest, most of the combined magnetic particles are gathered at the splicing line 12, and the increased magnetic field is helpful for the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 3
In embodiment 1, the first magnetic block 2 and the second magnetic block 3 are relatively static, and the reaction vessel 1 rises and swings in a zigzag manner, so that the combined magnetic particles are gathered at the bottom of the reaction vessel 1. The difference from the embodiment 1 is that, referring to fig. 6, the reaction vessel 1 is relatively static, and the first magnetic block 2 and the second magnetic block 3 respectively approach the first side wall 11 and the second side wall 12 of the reaction vessel 1 in a staggered manner and descend in a zigzag track; when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position and is close to the reaction vessel 1, the first magnetic block 2 or the second magnetic block 3 close to the reaction vessel 1 is far away from the reaction vessel 1, so that the combined magnetic particles are gathered at the bottom of the reaction vessel 1.
Specifically, referring to fig. 1 to 4 and 6, in order to thoroughly clean the object to be measured adsorbed on the surface of the combined magnetic particles or between the combined magnetic particles, at present, the combined magnetic particles are adsorbed or released to the cleaning solution by approaching and departing the same magnetic block and the reaction container, and the cleaning is realized by pumping waste liquid and filling the cleaning solution for many times, which has the defects of low cleaning efficiency, small moving range of the combined magnetic particles in the cleaning solution, resulting in incomplete cleaning of the combined magnetic particles in the cleaning solution, and the object to be measured may be partially adsorbed on the surface of the combined magnetic particles or sandwiched between the combined magnetic particles. In order to solve the technical problem, the reaction vessel 1 is relatively static, and the first magnetic block 2 and the second magnetic block 3 respectively approach the first side wall 11 and the second side wall 12 of the reaction vessel 1 in a staggered manner and descend in a zigzag track; specifically, when the first magnetic block 2 is attached to or close to the first outer wall 11 of the reaction vessel 1, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and then the first magnetic block 2 is away from the reaction vessel 1 in a folding line; when the second magnetic block 3 is attached to or close to the second outer wall 12 of the reaction vessel 1, the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12, and the second magnetic block 3 is far away from the reaction vessel 1 in a folding line; the first magnetic block 2 and the second magnetic block 3 alternately approach to the first side wall 11 and the second side wall 12 of the reaction vessel 1 according to the process and descend in a broken line track until the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel and approaches to the reaction vessel 1, and then horizontally moves away; in addition, the first magnetic block 2 and the second magnetic block 3 respectively approach the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered manner and descend in a broken line track, so that the combined magnetic particles shuttle in a reaction liquid in the reaction container in a broken line manner, in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container 1, the process of gathering, dispersing and re-gathering can occur, the shuttling track is in a broken line manner, and the shuttling track is shown in fig. 8, so that the shuttling stroke of the combined magnetic particles is greatly increased, the surfaces of the combined magnetic particles and the objects to be detected which are mixed between the combined magnetic particles and are not combined with the magnetic particles are cleaned, and the subsequent detection precision is not influenced by the objects to be detected which are not combined with the magnetic particles.
It should be noted that, when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel 1, where the lowest position means that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1, the combined magnetic particles are already adsorbed to the lowest part of the reaction vessel 1, and the combined magnetic particles can be taken out in an extruding or sucking manner; the moving structures of the first magnetic block 2 and the second magnetic block 3 in this embodiment are not important in the present invention, and the moving structures may be designed conventionally, for example, through a manipulator, a multi-degree-of-freedom guide rail, and other moving mechanisms, and the specific structures of the moving structures are not described in detail here, which does not affect understanding of those skilled in the art.
The technical solution disclosed in embodiment 3 has the same components as those in embodiment 1, please refer to embodiment 1, and will not be described herein again.
Example 4
On the basis of the embodiment 3, the embodiment 4 further comprises a third magnetic block 4, wherein the third magnetic block 4 is arranged at the bottom side of the reaction vessel 1; when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position and is close to the reaction vessel 1, the first magnetic block 2 or the second magnetic block 3 close to the reaction vessel 1 is far away from the reaction vessel 1, the third magnetic block 4 moves to the bottom of the reaction vessel 1, one side of the third magnetic block 4 is close to the bottom of the reaction vessel 1, and combined magnetic particles are gathered at the bottom of the reaction vessel 1.
Specifically, in example 3, although the bonded magnetic particles are adsorbed to the bottommost portion of the reaction vessel 1, since the first magnetic block 2 or the second magnetic block 3 is located on the side of the reaction vessel 1, the bonded magnetic particles may be in a state of being aggregated to the side wall, and a small amount of the sample may be taken out when the bonded magnetic particles are extruded or taken out, the bonded magnetic particles are completely and unbiased accumulated in the bottommost portion of the reaction vessel 1 by the third magnetic block 4 provided on the bottom side of the reaction vessel 1, and the sample is not taken out when the bonded magnetic particles are extruded or taken out.
As a preferred embodiment, the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 are all rectangular, circular, or irregular in shape, and the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 are respectively formed by splicing a plurality of magnetic blocks. Specifically, no matter the shape of the magnetic block is circular or rectangular, the combined magnetic particles are gathered and distributed on the corresponding inner wall of the magnetic block; referring to fig. 7, for example, two magnetic blocks are spliced, and the splicing lines are respectively located in the middle of the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4. Specifically, for the spliced magnetic block, the magnetic field at the splicing line 14 is strongest, most of the combined magnetic particles are gathered at the splicing line 12, and the increased magnetic field is helpful for the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 5
FIG. 9 shows a method for washing magnetic particles in an immunoassay device according to an embodiment of the present invention.
A method for magnetic particle washing of an immunoassay device, see flow diagram 9, comprising the steps of:
s1: a reaction solution containing an object to be measured and magnetic particles is disposed in a reaction vessel, and the magnetic particles are bonded to the object to be measured to form bonded magnetic particles. Specifically, the analyte is one of an antigen, a hapten, an antibody, a hormone and the like, the analyte reacts with the magnetic particles to form combined magnetic particles, the analyte which is not combined with the magnetic particles needs to be cleaned, the combined magnetic particles are the basis for subsequent detection, the combined magnetic particles need to be cleaned, and if the analyte is attached to the surface of the combined magnetic particles, the accuracy of the subsequent detection is affected.
S2: the reaction vessel swings between the first magnetic block and the second magnetic block which are fixed relatively and rises in a zigzag track. Specifically, the reaction vessel 1 is driven by the moving mechanism to swing between the first magnetic block 2 and the second magnetic block 3 and ascend in a zigzag track, specifically, when the reaction vessel 1 swings to the first magnetic block 2, the first side wall 11 of the reaction vessel 1 is attached to or close to the first magnetic block 2, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and when the reaction vessel 1 swings to the second magnetic block 3, the second side wall 12 of the reaction vessel 1 is attached to or close to the second magnetic block 3, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12; in addition, the reaction container 1 rises along a broken line track while swinging, so that the combined magnetic particles shuttle in a broken line in a reaction liquid in the reaction container, and in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of two sides of the reaction container 1, so that the processes of gathering, dispersing and re-gathering can occur, and the shuttle in a broken line is adopted, so that the shuttling stroke of the combined magnetic particles is greatly increased, the cleaning of the surfaces of the combined magnetic particles and the objects to be detected which are mixed between the combined magnetic particles and are not combined with the magnetic particles is realized, and the influence of the objects to be detected which are not combined with the magnetic particles on the subsequent detection precision is avoided.
S3: and when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container. Specifically, after the reaction vessel 1 rises to the highest position, the reaction vessel stops rising, where the highest position means that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1; the reaction vessel 1 descends and moves to the third magnetic block 4, the bottom of the reaction vessel 1 is close to one side of the third magnetic block 4, and the combined magnetic particles are gathered at the bottom of the reaction vessel 1.
Referring to fig. 8, the bottom of the reaction vessel 1 is tapered, the bottom of the reaction vessel 1 is provided with a micro-hole, and the top of the reaction vessel 1 is provided with a pipette 13. Specifically, the pipette tip of the pipette 13 is inserted into the inlet of the reaction vessel 1 and sealed so that the reaction solution does not flow out of the micro-hole of the reaction vessel 1, and when the bonded magnetic fine particles are adsorbed to the lowest conical tip by the washing device, the pipette 13 is controlled to discharge the lowest bonded magnetic fine particles in the reaction vessel 1 through the micro-hole, and the discharged bonded magnetic fine particles are measured.
As a preferred embodiment, the shapes of the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are all rectangular, circular or irregular. Specifically, no matter the shape of the magnetic block is circular or rectangular, the combined magnetic particles will be gathered and distributed on the corresponding inner wall of the magnetic block.
As a preferred embodiment, referring to fig. 7, the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 are respectively formed by splicing a plurality of magnetic blocks. Specifically, the splicing line 14 is respectively located in the middle of the first magnetic block, the second magnetic block and the third magnetic block, the magnetic field at the splicing line 14 of the spliced magnetic blocks is strongest, for example, when the two magnetic blocks are spliced, most of the combined magnetic particles are gathered at the splicing line 12, and the increased magnetic field is helpful for the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 6
In embodiment 5, the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are relatively static, while the reaction vessel 1 ascends and swings in a zigzag manner, and finally moves and descends to the third magnetic block 4, so that the bottom of the reaction vessel 1 is close to one side of the third magnetic block 4, and the combined magnetic particles are gathered at the bottom of the reaction vessel 1. The difference from the embodiment 5 is that, referring to fig. 10, the reaction vessel 1 is relatively static, and the first magnetic block 2 and the second magnetic block 3 alternately approach the first side wall 11 and the second side wall 12 of the reaction vessel 1 and descend in a zigzag track respectively. The method specifically comprises the following steps:
s4: a reaction solution containing an object to be measured and magnetic particles is disposed in a reaction vessel, and the magnetic particles are bonded to the object to be measured to form bonded magnetic particles. Specifically, the analyte is one of an antigen, a hapten, an antibody, a hormone and the like, the analyte reacts with the magnetic particles to form combined magnetic particles, the analyte which is not combined with the magnetic particles needs to be cleaned, the combined magnetic particles are the basis for subsequent detection, the combined magnetic particles need to be cleaned, and if the analyte is attached to the surface of the combined magnetic particles, the accuracy of the subsequent detection is affected.
S5: the first magnetic block and the second magnetic block which are arranged on two sides of the relatively fixed reaction container are close to the side wall of the reaction container in a staggered mode and descend in a broken line track. Specifically, when the first magnetic block 2 is attached to or close to the first outer wall 11 of the reaction vessel 1, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and then the first magnetic block 2 is away from the reaction vessel 1 in a folding line; when the second magnetic block 3 is attached to or close to the second outer wall 12 of the reaction vessel 1, the combined magnetic particles are adsorbed to the corresponding inner wall of the second side wall 12, and the second magnetic block 3 is far away from the reaction vessel 1 in a folding line; the first magnetic block 2 and the second magnetic block 3 alternately approach to the first side wall 11 and the second side wall 12 of the reaction vessel 1 according to the process and descend in a broken line track until the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel and approaches to the reaction vessel 1, and then horizontally moves away; in addition, the first magnetic block 2 and the second magnetic block 3 respectively approach the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered manner and descend in a broken line track, so that the combined magnetic particles shuttle in a reaction liquid in the reaction container in a broken line manner, and in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container 1, so that the gathering, dispersing and reaggregating processes can occur, and the shuttle in a broken line manner is adopted, so that the shuttling stroke of the combined magnetic particles is greatly increased, the surfaces of the combined magnetic particles and an object to be detected which is not combined with the magnetic particles and is mixed between the combined magnetic particles are cleaned, and the subsequent detection precision is prevented from being influenced by the object to be detected which is not combined with the magnetic particles.
S6: after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, the third magnetic block moves to the bottom of the reaction container, one side of the third magnetic block is close to the bottom of the reaction container, and combined magnetic particles are gathered at the bottom of the reaction container. Specifically, when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel 1, the lowest position here means that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1; when one side of the third magnetic block 4 is close to the bottom of the reaction vessel 1, the combined magnetic particles are gathered at the bottom of the reaction vessel, so that preparation is made for subsequent transfer of the combined magnetic particles.
It should be noted that the moving structures of the first magnetic block 2, the second magnetic block 3, and the third magnetic block 4 in this embodiment are not important in the present invention, and the moving structures may be designed conventionally, for example, by using a manipulator, a multi-degree-of-freedom guide rail, and other moving mechanisms, and the specific structures of the moving structures are not described in detail here, which does not affect understanding of those skilled in the art.
The technical solution disclosed in embodiment 6 has the same components as those in embodiment 5, please refer to embodiment 5, and will not be described herein again.
Claims (10)
1. The magnetic particle cleaning device for the immunoassay equipment is characterized by comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side surface of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a broken line track, and stops rising when rising to the highest position.
2. The magnetic particle washing device for an immunoassay apparatus according to claim 1, further comprising a third magnetic block disposed at a bottom side of the reaction vessel;
and when the reaction container rises to the highest position, stopping rising, moving the reaction container to the third magnetic block, enabling the bottom of the reaction container to be close to one side of the third magnetic block, and enabling the combined magnetic particles to be gathered at the bottom of the reaction container.
3. The magnetic particle cleaning device for the immunoassay equipment is characterized by comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side surface of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the first magnetic block and the second magnetic block are respectively close to the first side wall and the second side wall of the reaction container in a staggered mode and descend in a broken line track, and the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container.
4. The magnetic particle washing apparatus for an immunoassay device according to claim 3, further comprising a third magnetic block disposed at a bottom side of the reaction vessel;
when the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, the third magnetic block moves to the bottom of the reaction container, one side of the third magnetic block is close to the bottom of the reaction container, and combined magnetic particles are gathered at the bottom of the reaction container.
5. The magnetic particle washing apparatus for an immunological analytical device according to any one of claims 1 to 4, wherein the reaction vessel is provided with a micro-well at a bottom thereof and a pipette at a top thereof.
6. The magnetic particle cleaning device for the immunoassay device as defined in claim 2 or 4, wherein the first magnetic block, the second magnetic block and the third magnetic block are all rectangular, circular or irregular in shape, and the first magnetic block, the second magnetic block and the third magnetic block are respectively formed by splicing a plurality of magnetic blocks.
7. The magnetic particle cleaning method of the immunoassay device is characterized by comprising the following steps:
a reaction solution containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
the reaction vessel swings between the first magnetic block and the second magnetic block which are relatively fixed and rises in a broken line track;
and when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container.
8. The magnetic particle cleaning method of the immunoassay device is characterized by comprising the following steps:
a reaction solution containing an object to be detected and magnetic particles is configured in a reaction container, and the magnetic particles and the object to be detected are combined to form combined magnetic particles;
the first magnetic block and the second magnetic block which are arranged at two sides of the relatively fixed reaction vessel are close to the side wall of the reaction vessel in a staggered manner and descend in a broken line track;
after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, the third magnetic block moves to the bottom of the reaction container, one side of the third magnetic block is close to the bottom of the reaction container, and combined magnetic particles are gathered at the bottom of the reaction container.
9. The method for washing magnetic microparticles in an immunoassay device according to claim 7 or 8, wherein the bottom of the reaction vessel is tapered, the bottom of the reaction vessel is provided with a micro well, and the top of the reaction vessel is provided with a pipette.
10. The method for cleaning magnetic particles of immunoassay equipment as defined in claim 8, wherein the first magnetic block, the second magnetic block and the third magnetic block are all rectangular, circular or irregular in shape, and the first magnetic block, the second magnetic block and the third magnetic block are respectively formed by splicing a plurality of magnetic blocks.
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