CN115254834B - Magnetic particle cleaning device and method for immunoassay equipment - Google Patents
Magnetic particle cleaning device and method for immunoassay equipment Download PDFInfo
- Publication number
- CN115254834B CN115254834B CN202210922246.9A CN202210922246A CN115254834B CN 115254834 B CN115254834 B CN 115254834B CN 202210922246 A CN202210922246 A CN 202210922246A CN 115254834 B CN115254834 B CN 115254834B
- Authority
- CN
- China
- Prior art keywords
- magnetic
- magnetic block
- reaction container
- reaction
- combined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 194
- 238000004140 cleaning Methods 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000003018 immunoassay Methods 0.000 title claims description 22
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims abstract description 13
- 230000000630 rising effect Effects 0.000 claims description 8
- 230000008569 process Effects 0.000 abstract description 17
- 238000004458 analytical method Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 description 20
- 238000013459 approach Methods 0.000 description 18
- 239000007788 liquid Substances 0.000 description 11
- 239000002699 waste material Substances 0.000 description 6
- 238000005086 pumping Methods 0.000 description 5
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The application aims to provide a magnetic particle cleaning device and a cleaning method for immune analysis equipment, wherein the magnetic particle cleaning device comprises a reaction container, a first magnetic block, a second magnetic block and a third magnetic block; the bottom of the reaction vessel is conical; the first magnetic block is arranged on the side surface of the first side wall, the second magnetic block is arranged on one side of the second side wall, and the third magnetic block is arranged on the bottom side of the reaction container; the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a broken line track; the bottom of the reaction container is close to one side of the third magnetic block, the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and ascends in a fold line track, so that the combined magnetic particles shuttle in a reaction liquid in the reaction container in a fold line manner, and in the shuttle process in the reaction liquid, the surfaces of the combined magnetic particles and the objects to be detected which are mixed between the combined magnetic particles and are not combined with the magnetic particles are cleaned.
Description
Technical Field
The application relates to the technical field of immunoassay equipment, in particular to a magnetic particle cleaning device and a magnetic particle cleaning method for the immunoassay equipment.
Background
The immunoassay device is used for detection and analysis technology of various antigens, hapten, antibody, hormone and other analytes. The magnetic particle chemiluminescence immunoassay is a marked immunoassay technology for detecting trace amounts of an object to be detected, and the combined magnetic particles are a combination body of the magnetic particles and the object to be detected after reaction. The detection result accuracy is still affected when the detection is carried out on the detection object which is not combined with the magnetic particles, so that the detection accuracy is not affected by the detection object which is not combined with the magnetic particles, in particular, the detection object which is attached to the surfaces of the combined magnetic particles or is mixed between the combined magnetic particles and is not combined with the magnetic particles is cleaned, and the detection accuracy is prevented from being affected by the detection object which is not combined with the magnetic particles.
In the existing combined magnetic particle cleaning device, after a certain amount of liquid is mixed between an object to be tested and magnetic particles in a reaction container, the object to be tested reacts with the magnetic particles to form combined magnetic particles, the reaction container is moved to a magnet position, the magnet adsorbs and gathers the combined magnetic particles in the reaction container to one side of the inner wall of the container, the liquid in the reaction container is sucked and cleaned through a waste pumping needle, then cleaning liquid is filled after the reaction container leaves the magnet, the gathered combined magnetic particles are dispersed to a uniform state, the reaction container is moved to the magnet position on the same side or the same side, after the combined magnetic particles in the reaction container are adsorbed and gathered again, the liquid in the reaction container is sucked and cleaned again through the waste pumping needle, and the combined magnetic particles are cleaned after the actions are repeated for a plurality of times.
The cleaning device and the cleaning mode are easy to clean incompletely, especially the objects to be tested which are attached to the surfaces of the combined magnetic particles or clamped between the combined magnetic particles, and the residual objects to be tested can influence the subsequent detection precision; meanwhile, because of the processes of waste liquid pumping and cleaning liquid filling, on one hand, the liquid path system of the system is complicated, and in addition, waste liquid is easy to cause and pollute the environment.
In view of the above, there is a need for improvements in the existing cleaning apparatus and cleaning methods to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the technical problems, the application aims to provide a magnetic particle cleaning device and a cleaning method for immunoassay equipment, which can thoroughly clean combined magnetic particles through moving cooperation between a plurality of magnetic blocks and a reaction container.
In order to achieve the first object, the present application provides a magnetic particle cleaning apparatus for an immunoassay device, comprising a reaction vessel, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side face of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a broken line track, and the reaction container stops rising after rising to the highest position.
Preferably, the reaction vessel further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
and stopping lifting after the reaction container is lifted to the highest position, and moving and descending the reaction container to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container.
In order to achieve the first object, the present application further provides a magnetic particle cleaning apparatus for an immunoassay device, including a reaction vessel, a first magnetic block, and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side face of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the first magnetic blocks and the second magnetic blocks are respectively close to the first side wall and the second side wall of the reaction container in a staggered mode and descend in a broken line track, and the lowest descending position of the first magnetic blocks or the second magnetic blocks is close to the reaction container.
Preferably, the reaction vessel further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
when the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, and the third magnetic block moves to the bottom of the reaction container, so that one side of the third magnetic block is close to the bottom of the reaction container, and the combined magnetic particles are gathered at the bottom of the reaction container.
Preferably, the bottom of the reaction vessel is provided with a micro hole, and the top of the reaction vessel is provided with a pipette.
Preferably, the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or irregularly-shaped, and are formed by splicing a plurality of magnetic blocks respectively.
To achieve the second object, the present application provides a method for cleaning magnetic particles of an immunoassay device, comprising the steps of:
preparing a reaction solution comprising an object to be detected and magnetic particles in a reaction container, wherein the magnetic particles are combined with the object to be detected to form combined magnetic particles;
the reaction container swings between the first magnetic block and the second magnetic block which are relatively fixed and rises in a broken line track;
when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container.
In order to achieve the second object, the present application also provides a method for cleaning magnetic particles of an immunoassay device, comprising the steps of:
preparing a reaction solution comprising an object to be detected and magnetic particles in a reaction container, wherein the magnetic particles are combined with the object to be detected to form combined magnetic particles;
the first magnetic blocks and the second magnetic blocks which are arranged at two sides of the relatively fixed reaction container are staggered to be close to the side wall of the reaction container and descend in a broken line track;
after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, and the third magnetic block moves to the bottom of the reaction container, so that one side of the third magnetic block is close to the bottom of the reaction container, and the combined magnetic particles are gathered at the bottom of the reaction container.
Preferably, the bottom of the reaction container is conical, a micro hole is arranged at the bottom of the reaction container, and a liquid transfer device is arranged at the top of the reaction container.
Preferably, the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or special-shaped, and the first magnetic block, the second magnetic block and the third magnetic block are formed by splicing a plurality of two magnetic blocks respectively.
Through the technical scheme, the technical effects are as follows:
(1) The reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a fold line track, specifically, when the reaction container swings to the first magnetic block, the first side wall of the reaction container is attached to or close to the first magnetic block, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall, and when the reaction container swings to the second magnetic block, the second side wall of the reaction container is attached to or close to the second magnetic block, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall; in addition, the reaction container swings and rises along a broken line track, so that the combined magnetic particles shuttle along the broken line in the reaction liquid in the reaction container, and the surface of the combined magnetic particles and the objects which are mixed among the combined magnetic particles and are not combined with the magnetic particles are cleaned in the shuttle process in the reaction liquid, so that the objects which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
(2) The first magnetic blocks and the second magnetic blocks respectively approach the first side wall and the second side wall of the reaction container in a staggered manner and descend in a broken line track, and specifically, when the first magnetic blocks are attached to or approach the first outer wall of the reaction container, the first magnetic blocks are far away from the reaction container in a broken line after the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall; when the second magnetic block is attached to or close to the second outer wall of the reaction container, after the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall, the second magnetic block is far away from the reaction container in a fold line; the first magnetic block and the second magnetic block approach the first side wall and the second side wall of the reaction container in a staggered way according to the process and descend in a broken line track until the first magnetic block or the second magnetic block descends to the lowest part of the reaction container and approaches the reaction container; the first magnetic blocks and the second magnetic blocks are respectively close to the first side wall and the second side wall of the reaction container in a staggered manner and descend in a fold line track, so that the combined magnetic particles are subjected to fold line shuttle in the reaction liquid in the reaction container, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container in the shuttle process in the reaction liquid, gathering, dispersing and re-gathering processes can occur, and the shuttle process is in a fold line shuttle, so that the shuttle stroke of the combined magnetic particles is greatly increased, and the cleaning of the surfaces of the combined magnetic particles and the objects to be tested which are mixed between the combined magnetic particles and are not combined with the magnetic particles is realized, so that the objects to be tested which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present application, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the relative positions of the reaction vessel and the magnetic block according to the present application.
FIG. 2 is a schematic top view of the relative positions of the reaction vessel and the magnet.
FIG. 3 is a schematic front view showing the relative positions of the reaction vessel and the magnetic block according to the present application.
FIG. 4 is a schematic side view of the relative positions of the reaction vessel and the magnet.
FIG. 5 is a schematic view of the movement path of the reaction vessel according to the present application.
FIG. 6 is a schematic diagram of a moving track of a magnetic block according to the present application.
FIG. 7 is a schematic view of a magnetic block according to the present application.
FIG. 8 is a schematic diagram of the movement track of magnetic particles after the combination of the present application.
FIG. 9 is a flow chart of the cleaning method of the present application.
FIG. 10 is a flow chart of the cleaning method of the present application.
Wherein, 1, a reaction container; 11. a first outer wall; 12. a second outer wall; 14. a pipette; 14. splicing lines; 15. a micro hole; 2. a first magnetic block; 3. a second magnetic block; 4. and a third magnetic block.
Detailed Description
The technical solution of the present application will be described in detail with reference to the specific embodiments, and it should be understood that these embodiments are only for illustrating the present application and not for limiting the scope of the present application, and that various modifications equivalent to the present application will fall within the scope of the appended claims after reading the present application.
In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.
Example 1
One embodiment of the magnetic particle cleaning apparatus for an immunoassay device of the present application as shown in fig. 1 to 5.
As shown in fig. 1 to 5, the magnetic particle cleaning apparatus for an immunoassay device includes a reaction vessel 1, a first magnetic block 2, a second magnetic block 3, and a third magnetic block 4; the bottom of the reaction vessel 1 is conical, and the reaction vessel 1 comprises a first side wall 12 and a second side wall 12; the first magnetic block 2 is arranged on the side surface of the first side wall 11, and the second magnetic block 3 is arranged on one side of the second side wall 12; the reaction vessel 1 swings between the first magnetic block 2 and the second magnetic block 3 under the drive of the moving mechanism and rises in a broken line track, and the reaction vessel 1 stops rising after rising to the highest position.
Specifically, referring to fig. 1 to 5, in order to thoroughly wash away the to-be-detected object adsorbed on the surface of the combined magnetic particles or between the combined magnetic particles, at present, the combined magnetic particles are adsorbed or released to the cleaning solution through the approach and the separation of the same magnetic block and the reaction container, and the cleaning is realized by multiple waste liquid pumping and filling the cleaning solution, so that the cleaning efficiency is low, the defect that the moving range of the combined magnetic particles in the cleaning solution is small, the combined magnetic particles are not thoroughly cleaned in the cleaning solution, and the to-be-detected object is also partially adsorbed on the surface of the combined magnetic particles or clamped between the combined magnetic particles. In order to solve the above technical problem, the reaction vessel 1 swings between the first magnetic block 2 and the second magnetic block 3 under the driving of the moving mechanism and rises in a zigzag track, specifically, when the reaction vessel 1 swings to the first magnetic block 2, the first side wall 11 of the reaction vessel 1 is attached to or near the first magnetic block 2, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and when the reaction vessel 1 swings to the second magnetic block 3, the second side wall 12 of the reaction vessel 1 is attached to or near the second magnetic block 3, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12; in addition, the reaction vessel 1 swings and rises along a fold line track, so that the combined magnetic particles are shuttled along the fold line in the reaction liquid in the reaction vessel, and in the process of shuttled in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls at two sides of the reaction vessel 1, aggregation, dispersion and reaggregation processes can occur, and the shuttled track is shuttled along the fold line, as shown in fig. 8, the shuttled travel of the combined magnetic particles is greatly increased, so that the surfaces of the combined magnetic particles and the objects to be detected which are mixed between the combined magnetic particles and are not combined with the magnetic particles are cleaned, and the influence of the objects to be detected which are not combined with the magnetic particles on the subsequent detection precision is avoided.
It should be noted that, when the reaction vessel 1 rises to the highest position, the rising is stopped, the highest position means that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1, and the combined magnetic particles are already adsorbed to the bottommost part of the reaction vessel 1, and the combined magnetic particles can be taken out by extrusion or suction; the moving mechanism in this embodiment drives the reaction vessel 1 to swing and lift in a broken line, and the specific structure of the moving mechanism is not important in the present application, and the moving mechanism may be designed conventionally, for example, by a mechanical arm, a multi-degree-of-freedom guide rail, etc., so that the specific structure of the moving mechanism will not be described in detail herein, and will not affect the understanding of those skilled in the art.
As a preferred embodiment, referring to fig. 8, the bottom of the reaction vessel 1 is provided with a micro-hole 15, and the top of the reaction vessel 1 is provided with a pipette 13. Specifically, the pipette head of the pipette 13 is inserted into the inlet of the reaction vessel 1 and sealed so that the reaction liquid does not flow out from the micro-hole of the reaction vessel 1, and when the bonded magnetic particles are adsorbed to the conical head at the lowest end by the above-mentioned cleaning device, the pipette 13 is controlled to discharge the bonded magnetic particles at the lowest end in the reaction vessel 1 through the micro-hole, and the discharged bonded magnetic particles are to be measured.
Example 2
On the basis of embodiment 1, embodiment 2 further comprises a third magnetic block 4, wherein the third magnetic block 4 is disposed at the bottom side of the reaction container 1; when the reaction vessel 1 is lifted to the highest position, the lifting is stopped, the reaction vessel 1 moves down to the third magnetic block 4, and the bottom of the reaction vessel 1 is close to one side of the third magnetic block 4, so that the combined magnetic particles are gathered at the bottom of the reaction vessel 1, and the method is specifically shown in fig. 4.
Specifically, in example 1, although the bonded magnetic particles are adsorbed to the bottommost portion of the reaction vessel 1, since the bonded magnetic particles are aggregated to the side wall in view of the fact that the first magnetic block 2 or the second magnetic block 3 is located laterally of the reaction vessel 1, a small amount of the sample may be carried out when the bonded magnetic particles are extruded or taken out, and therefore, the bonded magnetic particles are completely and unbiased aggregated to the bottommost portion of the reaction vessel 1 by the third magnetic block 4 provided on the bottom side of the reaction vessel 1, and the sample is not carried out when the bonded magnetic particles are extruded or taken out.
As a preferred embodiment, the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are rectangular, circular or special-shaped, and the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are formed by splicing a plurality of magnetic blocks respectively. Specifically, no matter whether the shape of the magnetic block is round or rectangular, the combined magnetic particles are distributed on the inner wall corresponding to the magnetic block in a gathering way; referring to fig. 7, taking two magnet blocks for example, the splicing lines are respectively located in the middle of the first magnet block 2, the second magnet block 3 and the third magnet block 4. Specifically, for the spliced magnetic block, the magnetic field at the spliced line 14 is strongest, most of the combined magnetic particles are gathered at the spliced line 12, and the increased magnetic field is helpful to the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 3
In embodiment 1, the first magnetic block 2 and the second magnetic block 3 are relatively stationary, and the reaction vessel 1 swings upward in a zigzag shape, so that the combined magnetic particles are gathered at the bottom of the reaction vessel 1. The difference from embodiment 1 is that, referring to fig. 6, the reaction vessel 1 is relatively stationary, and the first magnetic blocks 2 and the second magnetic blocks 3 approach the first side wall 11 and the second side wall 12 of the reaction vessel 1, respectively, in a staggered manner and descend in a zigzag track; when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position and approaches the reaction container 1, the first magnetic block 2 or the second magnetic block 3 approaching the reaction container 1 is far away from the reaction container 1, so that the combined magnetic particles are gathered at the bottom of the reaction container 1.
Specifically, referring to fig. 1 to 4 and 6, in order to thoroughly wash away the to-be-detected object adsorbed on the surface of the combined magnetic particles or between the combined magnetic particles, at present, the combined magnetic particles are adsorbed or released to the cleaning solution through the approach and the separation of the same magnetic block and the reaction container, and the cleaning is realized by pumping waste liquid and filling the cleaning solution for multiple times, so that the cleaning efficiency is low, the defect that the moving range of the combined magnetic particles in the cleaning solution is small, the combined magnetic particles are not thoroughly cleaned in the cleaning solution, and the to-be-detected object is also partially adsorbed on the surface of the combined magnetic particles or clamped between the combined magnetic particles. In order to solve the above technical problem, the reaction vessel 1 is relatively stationary, and the first magnetic blocks 2 and the second magnetic blocks 3 respectively approach the first side wall 11 and the second side wall 12 of the reaction vessel 1 in a staggered manner and descend in a zigzag track; specifically, when the first magnetic block 2 is attached to or near the first outer wall 11 of the reaction container 1, after the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, the first magnetic block 2 is far away from the reaction container 1 in a fold line; when the second magnetic block 3 is attached to or near the second outer wall 12 of the reaction vessel 1, after the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12, the second magnetic block 3 is far away from the reaction vessel 1 in a fold line; the first magnetic block 2 and the second magnetic block 3 approach the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered way according to the process and descend in a broken line track until the first magnetic block 2 or the second magnetic block 3 descends to the lowest part of the reaction container and approaches the reaction container 1 and then horizontally moves away; in addition, the first magnetic blocks 2 and the second magnetic blocks 3 are respectively close to the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered manner and descend in a zigzag track, so that the combined magnetic particles are zigzag shuttled in the reaction liquid in the reaction container, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container 1 in the shuttling process in the reaction liquid, the gathering, dispersing and re-gathering processes can occur, and the zigzag shuttling is performed, and the shuttling track is shown in fig. 8, so that the shuttling travel of the combined magnetic particles is greatly increased, and the surface of the combined magnetic particles and the objects to be tested which are mixed among the combined magnetic particles and are not combined with the magnetic particles are cleaned, so that the objects to be tested which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
It should be noted that, when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel 1, the lowest position refers to that the bottom end of the first magnetic block 2 or the second magnetic block 3 is already close to the lowest end of the reaction vessel 1, and the combined magnetic particles are already adsorbed to the bottommost part of the reaction vessel 1 at this time, and the combined magnetic particles can be taken out by extrusion or suction; the moving structure of the first magnetic block 2 and the second magnetic block 3 in this embodiment is not an important point of the present application, and the moving structure may be a conventional design, for example, a moving mechanism such as a manipulator, a multi-degree-of-freedom rail, etc., so that the specific structure of the moving structure is not described herein, and the understanding of those skilled in the art is not affected.
The technical solution disclosed in embodiment 3 has the same parts as those in embodiment 1, please refer to embodiment 1 for description, and the description is omitted here.
Example 4
On the basis of embodiment 3, embodiment 4 further comprises a third magnetic block 4, wherein the third magnetic block 4 is disposed at the bottom side of the reaction container 1; when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position and approaches the reaction container 1, the first magnetic block 2 or the second magnetic block 3 approaching the reaction container 1 is far away from the reaction container 1, and the third magnetic block 4 moves to the bottom of the reaction container 1, so that one side of the third magnetic block 4 approaches the bottom of the reaction container 1, and the combined magnetic particles are gathered at the bottom of the reaction container 1.
Specifically, in example 3, although the bonded magnetic particles are adsorbed to the bottommost portion of the reaction vessel 1, since the bonded magnetic particles are aggregated to the side wall in view of the fact that the first magnetic block 2 or the second magnetic block 3 is located laterally of the reaction vessel 1, a small amount of the sample may be carried out when the bonded magnetic particles are extruded or taken out, and therefore, the bonded magnetic particles are completely and unbiased aggregated to the bottommost portion of the reaction vessel 1 by the third magnetic block 4 provided on the bottom side of the reaction vessel 1, and the sample is not carried out when the bonded magnetic particles are extruded or taken out.
As a preferred embodiment, the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are rectangular, circular or special-shaped, and the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are formed by splicing a plurality of magnetic blocks respectively. Specifically, no matter whether the shape of the magnetic block is round or rectangular, the combined magnetic particles are distributed on the inner wall corresponding to the magnetic block in a gathering way; referring to fig. 7, taking two magnet blocks for example, the splicing lines are respectively located in the middle of the first magnet block 2, the second magnet block 3 and the third magnet block 4. Specifically, for the spliced magnetic block, the magnetic field at the spliced line 14 is strongest, most of the combined magnetic particles are gathered at the spliced line 12, and the increased magnetic field is helpful to the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 5
An embodiment of the method for cleaning magnetic particles of an immunoassay device of the present application as shown in fig. 9.
A method for cleaning magnetic particles of an immunoassay device, see flowchart 9, comprising the steps of:
s1: a reaction solution containing the object to be tested and magnetic particles is arranged in a reaction container, and the magnetic particles and the object to be tested are combined to form combined magnetic particles. Specifically, the to-be-detected object is one of antigen, hapten, antibody, hormone and the like, the to-be-detected object reacts with the magnetic particles to form combined magnetic particles, the to-be-detected object which is not combined with the magnetic particles needs to be cleaned, the combined magnetic particles are the basis for subsequent detection, the combined magnetic particles need to be cleaned, and if the to-be-detected object is attached to the surface of the combined magnetic particles, the accuracy of subsequent detection is affected.
S2: the reaction container swings between the first magnetic block and the second magnetic block which are relatively fixed and rises in a fold line track. Specifically, the reaction container 1 swings between the first magnetic block 2 and the second magnetic block 3 under the drive of the moving mechanism and rises in a fold line track, specifically, when the reaction container 1 swings to the first magnetic block 2, the first side wall 11 of the reaction container 1 is attached to or close to the first magnetic block 2, the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, and when the reaction container 1 swings to the second magnetic block 3, the second side wall 12 of the reaction container 1 is attached to or close to the second magnetic block 3, and the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12; in addition, the reaction vessel 1 swings and rises along a fold line track, so that the combined magnetic particles are shuttled along the fold line in the reaction liquid in the reaction vessel, and in the process of shuttled in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls at two sides of the reaction vessel 1, aggregation, dispersion and reaggregation processes can occur, and the shuttled magnetic particles are shuttled along the fold line, so that the shuttle stroke of the combined magnetic particles is greatly increased, and the surface of the combined magnetic particles and the objects to be detected which are mixed among the combined magnetic particles and are not combined with the magnetic particles are cleaned, so that the objects to be detected which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
S3: when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container. Specifically, when the reaction vessel 1 is raised to the highest position, the raising is stopped, wherein the highest position means that the bottom end of the first magnetic block 2 or the second magnetic block 3 is close to the lowest end of the reaction vessel 1; the reaction vessel 1 is lowered and moved to the third magnetic block 4, so that the bottom of the reaction vessel 1 approaches one side of the third magnetic block 4, and the combined magnetic particles are collected at the bottom of the reaction vessel 1.
As a preferred embodiment, referring to fig. 8, the bottom of the reaction vessel 1 is tapered, the bottom of the reaction vessel 1 is provided with a micro-hole, and the top of the reaction vessel 1 is provided with a pipette 13. Specifically, the pipette head of the pipette 13 is inserted into the inlet of the reaction vessel 1 and sealed so that the reaction liquid does not flow out from the micro-hole of the reaction vessel 1, and when the bonded magnetic particles are adsorbed to the conical head at the lowest end by the above-mentioned cleaning device, the pipette 13 is controlled to discharge the bonded magnetic particles at the lowest end in the reaction vessel 1 through the micro-hole, and the discharged bonded magnetic particles are to be measured.
As a preferred embodiment, the shapes of the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are all rectangular, circular or special-shaped. Specifically, no matter the shape of the magnetic block is round or rectangular, the combined magnetic particles are distributed on the inner wall corresponding to the magnetic block in a gathering way.
As a preferred embodiment, referring to fig. 7, the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are respectively formed by splicing a plurality of magnetic blocks. Specifically, the splice lines 14 are respectively located at the middle parts of the first magnetic block, the second magnetic block and the third magnetic block, the magnetic field at the splice lines 14 of the spliced magnetic blocks is strongest, and taking two magnetic blocks for splicing as an example, most of the combined magnetic particles are gathered at the splice lines 12, and the increased magnetic field is helpful to the gathering speed of the combined magnetic particles, so that the cleaning efficiency is improved.
Example 6
In embodiment 5, the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 are relatively stationary, and the reaction vessel 1 swings upward in a zigzag shape, and finally moves down to the third magnetic block 4 and brings the bottom of the reaction vessel 1 close to one side of the third magnetic block 4, so that the combined magnetic particles are gathered at the bottom of the reaction vessel 1. The difference from example 5 is that, referring to fig. 10, the reaction vessel 1 is relatively stationary, and the first magnetic blocks 2 and the second magnetic blocks 3 approach the first side wall 11 and the second side wall 12 of the reaction vessel 1, respectively, alternately and descend in a zigzag track. The method specifically comprises the following steps:
s4: a reaction solution containing the object to be tested and magnetic particles is arranged in a reaction container, and the magnetic particles and the object to be tested are combined to form combined magnetic particles. Specifically, the to-be-detected object is one of antigen, hapten, antibody, hormone and the like, the to-be-detected object reacts with the magnetic particles to form combined magnetic particles, the to-be-detected object which is not combined with the magnetic particles needs to be cleaned, the combined magnetic particles are the basis for subsequent detection, the combined magnetic particles need to be cleaned, and if the to-be-detected object is attached to the surface of the combined magnetic particles, the accuracy of subsequent detection is affected.
S5: the first magnetic blocks and the second magnetic blocks arranged at two sides of the relatively fixed reaction container are staggered to be close to the side wall of the reaction container and descend in a broken line track. Specifically, when the first magnetic block 2 is attached to or near the first outer wall 11 of the reaction container 1, after the combined magnetic particles are adsorbed to the inner wall corresponding to the first side wall 11, the first magnetic block 2 is far away from the reaction container 1 in a fold line; when the second magnetic block 3 is attached to or near the second outer wall 12 of the reaction vessel 1, after the combined magnetic particles are adsorbed to the inner wall corresponding to the second side wall 12, the second magnetic block 3 is far away from the reaction vessel 1 in a fold line; the first magnetic block 2 and the second magnetic block 3 approach the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered way according to the process and descend in a broken line track until the first magnetic block 2 or the second magnetic block 3 descends to the lowest part of the reaction container and approaches the reaction container 1 and then horizontally moves away; in addition, the first magnetic blocks 2 and the second magnetic blocks 3 are respectively close to the first side wall 11 and the second side wall 12 of the reaction container 1 in a staggered manner and descend in a zigzag track, so that the combined magnetic particles are zigzag shuttled in the reaction liquid in the reaction container, and in the process of shuttling in the reaction liquid, the combined magnetic particles are repeatedly adsorbed and gathered on the inner walls of the two sides of the reaction container 1, the gathering, dispersing and re-gathering processes can occur, and the zigzag shuttling is performed, so that the shuttle stroke of the combined magnetic particles is greatly increased, and the surface of the combined magnetic particles and the objects to be tested which are mixed between the combined magnetic particles and are not combined with the magnetic particles are cleaned, so that the objects to be tested which are not combined with the magnetic particles are prevented from influencing the subsequent detection precision.
S6: after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, and the third magnetic block moves to the bottom of the reaction container, so that one side of the third magnetic block is close to the bottom of the reaction container, and the combined magnetic particles are gathered at the bottom of the reaction container. Specifically, when the first magnetic block 2 or the second magnetic block 3 descends to the lowest position of the reaction vessel 1, the lowest position herein means that the bottom end of the first magnetic block 2 or the second magnetic block 3 has come close to the lowest end of the reaction vessel 1; when one side of the third magnetic block 4 approaches the bottom of the reaction container 1, the combined magnetic particles are gathered at the bottom of the reaction container, so that preparation is made for transferring the combined magnetic particles subsequently.
It should be noted that, the moving structures of the first magnetic block 2, the second magnetic block 3 and the third magnetic block 4 in this embodiment are not important to the present application, and the moving structures may be conventional, for example, by using a mechanical arm, a multi-degree-of-freedom guide rail, etc., so that the specific structures of the moving structures will not be described herein, and will not affect the understanding of those skilled in the art.
The technical solution disclosed in embodiment 6 has the same parts as those in embodiment 5, please refer to embodiment 5, and the description is omitted here.
Claims (6)
1. The magnetic particle cleaning device for the immunoassay equipment is characterized by comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side face of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the reaction container swings between the first magnetic block and the second magnetic block under the drive of the moving mechanism and rises in a broken line track, and the reaction container stops rising after rising to the highest position;
the combined magnetic particles are shuttled in a broken line in the reaction liquid in the reaction container;
the reaction vessel further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
stopping rising after the reaction container rises to the highest position, and moving the reaction container to descend to the third magnetic block, enabling the bottom of the reaction container to be close to one side of the third magnetic block, and enabling the combined magnetic particles to be gathered at the bottom of the reaction container;
the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or special-shaped, and are formed by splicing a plurality of magnetic blocks respectively.
2. The magnetic particle cleaning device for the immunoassay equipment is characterized by comprising a reaction container, a first magnetic block and a second magnetic block;
the bottom of the reaction vessel is conical, and the reaction vessel comprises a first side wall and a second side wall;
the first magnetic block is arranged on the side face of the first side wall, and the second magnetic block is arranged on one side of the second side wall;
the first magnetic blocks and the second magnetic blocks are respectively close to the first side wall and the second side wall of the reaction container in a staggered manner and descend in a broken line track, and the lowest descending position of the first magnetic blocks or the second magnetic blocks is close to the reaction container;
the combined magnetic particles are shuttled in a broken line in the reaction liquid in the reaction container;
the reaction vessel further comprises a third magnetic block, wherein the third magnetic block is arranged at the bottom side of the reaction vessel;
when the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, and the third magnetic block moves to the bottom of the reaction container, so that one side of the third magnetic block is close to the bottom of the reaction container, and the combined magnetic particles are gathered at the bottom of the reaction container;
the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or special-shaped, and are formed by splicing a plurality of magnetic blocks respectively.
3. The magnetic particle cleaning apparatus for an immunoassay device according to any one of claims 1 to 2, wherein a micro-well is provided at the bottom of the reaction vessel, and a pipette is provided at the top of the reaction vessel.
4. A method for cleaning magnetic particles of an immunoassay device, comprising the steps of:
preparing a reaction solution comprising an object to be detected and magnetic particles in a reaction container, wherein the magnetic particles are combined with the object to be detected to form combined magnetic particles;
the reaction container swings between the first magnetic block and the second magnetic block which are relatively fixed and rises in a broken line track;
when the reaction container rises to the highest position, the reaction container descends and moves to the third magnetic block, so that the bottom of the reaction container is close to one side of the third magnetic block, and the combined magnetic particles are gathered at the bottom of the reaction container;
the combined magnetic particles are shuttled in a broken line in the reaction liquid in the reaction container;
the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or special-shaped, and are formed by splicing a plurality of magnetic blocks respectively.
5. A method for cleaning magnetic particles of an immunoassay device, comprising the steps of:
preparing a reaction solution comprising an object to be detected and magnetic particles in a reaction container, wherein the magnetic particles are combined with the object to be detected to form combined magnetic particles;
the first magnetic blocks and the second magnetic blocks which are arranged at two sides of the relatively fixed reaction container are staggered to be close to the side wall of the reaction container and descend in a broken line track;
after the first magnetic block or the second magnetic block descends to the lowest position and is close to the reaction container, the first magnetic block or the second magnetic block close to the reaction container is far away from the reaction container, and the third magnetic block moves to the bottom of the reaction container, so that one side of the third magnetic block is close to the bottom of the reaction container, and the combined magnetic particles are gathered at the bottom of the reaction container;
the combined magnetic particles are shuttled in a broken line in the reaction liquid in the reaction container;
the first magnetic block, the second magnetic block and the third magnetic block are rectangular, circular or special-shaped, and are formed by splicing a plurality of magnetic blocks respectively.
6. The method for cleaning magnetic particles of an immunoassay device according to claim 4 or 5, wherein the bottom of the reaction vessel is tapered, the bottom of the reaction vessel is provided with a micro-hole, and the top of the reaction vessel is provided with a pipette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210922246.9A CN115254834B (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and method for immunoassay equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210922246.9A CN115254834B (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and method for immunoassay equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115254834A CN115254834A (en) | 2022-11-01 |
| CN115254834B true CN115254834B (en) | 2023-10-27 |
Family
ID=83746835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210922246.9A Active CN115254834B (en) | 2022-08-02 | 2022-08-02 | Magnetic particle cleaning device and method for immunoassay equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115254834B (en) |
Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0580052A (en) * | 1991-09-20 | 1993-03-30 | Nippon Paint Co Ltd | Apparatus and method for measuring substance in vivo |
| WO1996026011A1 (en) * | 1995-02-21 | 1996-08-29 | Siddiqi Iqbal W | Apparatus and method for mixing and separation employing magnetic particles |
| TW200300365A (en) * | 2001-11-19 | 2003-06-01 | Chemagen Biopolymer Technologie Ag | Device and methods for treating magnetic particles |
| JP2006112824A (en) * | 2004-10-12 | 2006-04-27 | Terametsukusu Kk | B/f separation method in automatic immunoassay device |
| JP2010151738A (en) * | 2008-12-26 | 2010-07-08 | Tosoh Corp | Method and device for separating magnetic particle |
| CN102066934A (en) * | 2008-06-12 | 2011-05-18 | 株式会社日立高新技术 | Analyzer using magnetic particles |
| CN102466732A (en) * | 2010-11-18 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
| TW201311905A (en) * | 2011-09-14 | 2013-03-16 | Univ Kun Shan | Automatization mechanism for liquid chip cleaning and luminescence detection |
| JP2013088164A (en) * | 2011-10-14 | 2013-05-13 | Hitachi High-Technologies Corp | Automatic analyzer |
| CN103760373A (en) * | 2014-02-21 | 2014-04-30 | 中国科学院苏州生物医学工程技术研究所 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
| CN103861735A (en) * | 2014-03-19 | 2014-06-18 | 东南大学 | Magnetic separation device |
| CN203737677U (en) * | 2014-02-24 | 2014-07-30 | 湖南康博生物科技有限公司 | Magnetic bead swimming motility washing device for chemical luminescence immunity analyzer |
| CN107107021A (en) * | 2015-01-15 | 2017-08-29 | 株式会社岛津制作所 | The operating method of magnetic particle manipulation element and magnetic particle |
| CN107626702A (en) * | 2017-08-28 | 2018-01-26 | 重庆科斯迈生物科技有限公司 | Cup bar is to the double washing systems of suction and its washing methods |
| JP2019100976A (en) * | 2017-12-07 | 2019-06-24 | 株式会社日立ハイテクノロジーズ | Magnetic separation method and automatic analyzer |
| WO2020041922A1 (en) * | 2018-08-27 | 2020-03-05 | 深圳迎凯生物科技有限公司 | Immune analyzer and cleaning device therefor |
| CN211246938U (en) * | 2019-11-15 | 2020-08-14 | 南京泰斯德生物科技有限公司 | Magnetic separation device |
| CN111855995A (en) * | 2020-08-03 | 2020-10-30 | 宋世琦 | Incubation device |
| CN112067829A (en) * | 2019-06-10 | 2020-12-11 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleaning and separating device and method and chemiluminescence immunoassay analyzer |
| CN214585467U (en) * | 2021-03-31 | 2021-11-02 | 中科堃腾仪器有限公司 | Full-automatic high flux immunomagnetic bead separator |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090027998A1 (en) * | 2007-07-25 | 2009-01-29 | Abbott Laboratories | Magnetic mixer |
-
2022
- 2022-08-02 CN CN202210922246.9A patent/CN115254834B/en active Active
Patent Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0580052A (en) * | 1991-09-20 | 1993-03-30 | Nippon Paint Co Ltd | Apparatus and method for measuring substance in vivo |
| WO1996026011A1 (en) * | 1995-02-21 | 1996-08-29 | Siddiqi Iqbal W | Apparatus and method for mixing and separation employing magnetic particles |
| TW200300365A (en) * | 2001-11-19 | 2003-06-01 | Chemagen Biopolymer Technologie Ag | Device and methods for treating magnetic particles |
| JP2006112824A (en) * | 2004-10-12 | 2006-04-27 | Terametsukusu Kk | B/f separation method in automatic immunoassay device |
| CN102066934A (en) * | 2008-06-12 | 2011-05-18 | 株式会社日立高新技术 | Analyzer using magnetic particles |
| JP2010151738A (en) * | 2008-12-26 | 2010-07-08 | Tosoh Corp | Method and device for separating magnetic particle |
| CN102466732A (en) * | 2010-11-18 | 2012-05-23 | 南京神州英诺华医疗科技有限公司 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
| TW201311905A (en) * | 2011-09-14 | 2013-03-16 | Univ Kun Shan | Automatization mechanism for liquid chip cleaning and luminescence detection |
| JP2013088164A (en) * | 2011-10-14 | 2013-05-13 | Hitachi High-Technologies Corp | Automatic analyzer |
| CN103760373A (en) * | 2014-02-21 | 2014-04-30 | 中国科学院苏州生物医学工程技术研究所 | Cleaning device in fully-automatic chemiluminescence immunity analyzer |
| CN203737677U (en) * | 2014-02-24 | 2014-07-30 | 湖南康博生物科技有限公司 | Magnetic bead swimming motility washing device for chemical luminescence immunity analyzer |
| CN103861735A (en) * | 2014-03-19 | 2014-06-18 | 东南大学 | Magnetic separation device |
| CN107107021A (en) * | 2015-01-15 | 2017-08-29 | 株式会社岛津制作所 | The operating method of magnetic particle manipulation element and magnetic particle |
| CN107626702A (en) * | 2017-08-28 | 2018-01-26 | 重庆科斯迈生物科技有限公司 | Cup bar is to the double washing systems of suction and its washing methods |
| JP2019100976A (en) * | 2017-12-07 | 2019-06-24 | 株式会社日立ハイテクノロジーズ | Magnetic separation method and automatic analyzer |
| WO2020041922A1 (en) * | 2018-08-27 | 2020-03-05 | 深圳迎凯生物科技有限公司 | Immune analyzer and cleaning device therefor |
| CN112067829A (en) * | 2019-06-10 | 2020-12-11 | 成都深迈瑞医疗电子技术研究院有限公司 | Magnetic bead cleaning and separating device and method and chemiluminescence immunoassay analyzer |
| CN211246938U (en) * | 2019-11-15 | 2020-08-14 | 南京泰斯德生物科技有限公司 | Magnetic separation device |
| CN111855995A (en) * | 2020-08-03 | 2020-10-30 | 宋世琦 | Incubation device |
| CN214585467U (en) * | 2021-03-31 | 2021-11-02 | 中科堃腾仪器有限公司 | Full-automatic high flux immunomagnetic bead separator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115254834A (en) | 2022-11-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105974147B (en) | Blood group serology automatic detection all-in-one | |
| JP5795973B2 (en) | Tapered cuvette and method for collecting magnetic particles | |
| CN101529246B (en) | Assay apparatuses, methods and reagents | |
| JP2014130151A (en) | Assay apparatuses, methods and reagents | |
| US9545648B2 (en) | Magnetic particle washing apparatus and method | |
| CN107422138A (en) | A kind of rail mounted system for pretreating sample | |
| CN2847292Y (en) | Full automatic biochemical immunological analyzer | |
| CN115254834B (en) | Magnetic particle cleaning device and method for immunoassay equipment | |
| CN109799336B (en) | Dark box detection system of magnetic particle chemiluminescence immunoassay analyzer | |
| CN113820195B (en) | Multichannel parallel pretreatment device | |
| JP2014070915A (en) | Liquid sucking method, and solid phase washing method using the same | |
| JP2007212399A (en) | Automatic analyzer and sampling control method | |
| JP3853407B2 (en) | Automatic immunological analyzer | |
| CN218532130U (en) | ELISA plate cleaning device | |
| CN115327090A (en) | Platelet detection device | |
| CN105115949A (en) | Assay apparatuses, methods and reagents | |
| CN210907134U (en) | Micro-fluidic chip's belt cleaning device | |
| JPH0968536A (en) | Automatic analyzing device | |
| CN205844340U (en) | Blood group serology Aulomatizeted Detect all-in-one | |
| CN218629845U (en) | Automatic uncovering and sample adding device suitable for single-molecule POCT equipment and single-molecule POCT equipment comprising same | |
| US20230228685A1 (en) | Point-of-care testing (poct) fully-automatic chemiluminescence device based on multi-channel parallel pretreatment technology | |
| CN218445522U (en) | Platelet detection device | |
| JPS59120960A (en) | Automatic inserting and guiding method of suction nozzle | |
| JP2004223352A (en) | Cleaning apparatus | |
| CN217786685U (en) | Sample preparation device for cytokine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |