CN102466732A - Method for precisely absorbing micro magnetic particle with automatic analyzer - Google Patents
Method for precisely absorbing micro magnetic particle with automatic analyzer Download PDFInfo
- Publication number
- CN102466732A CN102466732A CN2010105489478A CN201010548947A CN102466732A CN 102466732 A CN102466732 A CN 102466732A CN 2010105489478 A CN2010105489478 A CN 2010105489478A CN 201010548947 A CN201010548947 A CN 201010548947A CN 102466732 A CN102466732 A CN 102466732A
- Authority
- CN
- China
- Prior art keywords
- magnetic particle
- little magnetic
- container
- magnetic particles
- little
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to a method for precisely absorbing micro magnetic particles with an automatic analyzer. The method comprises the following steps that a magnetic force device is adopted, and micro magnetic particles are uniformly and centrally distributed on the bottom of a container with the aid of particle setting, so that the automatic analyzer can automatically and precisely quantitatively absorb micro magnetic particles. The working process of the method is that a magnetic force generator arranged on the bottom of a container with micro magnetic particles is turned on so as to absorb micro magnetic particles to be centralized on the bottom of the container to form uniform distribution, then the magnetic force generator is turned off, and a sampling probe is inserted into the micro magnetic particle centralizing area on the bottom of the container to quantitatively absorb micro magnetic particles. Before the sampling probe absorbs micro magnetic particles again, the magnetic force generator is turned on again, so as to ensure that micro magnetic particles centralize on the bottom of the container to form uniform distribution, and ensure that the sampling probe can precisely and quantitatively absorb magnetic particles each time.
Description
One, technical field
(or claim: magnetic nanoparticle) application is more and more universal in nucleic acid extraction, check and analysis and immunology detection, and the present invention is a kind of technology of these molecules quantitatively being taked by instrument in the self-reacting device of little magnetic particle or other molecule that is applicable to for recent little magnetic particle.
Two, background technology
Present full-automatic detecting instrument applied more and more in laboratories such as medical science, molecular biology, immunology by little magnetic particle.And little magnetic particle (or similarly molecule) is generally deposited in the liquid because proportion or other influence of particle, usually make this slightly the magnetic particle in liquid, become uneven distribution.Added in a subtle way magnetic particle (or similarly molecule) in the testing process amount accurately by making; Before full-automatic appearance is got little magnetic particle; Instrument often designs evenly mixing device little magnetic particle is carried out mixing, realizes that little magnetic uniform particles distributes in the container, thus little magnetic amounts of particles that basic assurance is taked at every turn; Consistent with the requirement of expection, to guarantee that testing result is accurately and reliably.Instrument adopts the method for mixing to realize that little magnetic uniform particles distributes in the container, corresponding construction more greatly, complicated, failure rate is high, improve structural cost, and needs the long period mixing could realize little magnetic uniform particles distribution in the container.
Three, summary of the invention
Content of the present invention is that the Vessel Design of adorning little magnetic particle is become the bottom is taper, and the magnetic means that a gate-controlled switch (or movably magnet) is set under little magnetic container for granule is being housed.Before sampling probe is taked little magnetic particle, open gate-controlled switch (or move to container bottom with magnet), little magnetic particle is concentrated in the conical base of container.When the sampling syringe needle is assigned like the awl container bottom sampling, close gate-controlled switch (or move apart container bottom with magnet).Can overfulfil a production target a ripple or a photoelectric detector be installed at the container side in addition; This detecting device judges that through the decline that detects interface on little magnetic particle container bottom remains the quantity or the uniformity coefficient of little magnetic particle; The interface drops to a certain degree on little magnetic particle; Then stop to draw little magnetic particle, with little magnetic grain amount and the needed basically identical of guaranteeing that sampling probe is got.
Four, description of drawings
Fig. 1 is switch following time in off position, the magnetic attraction that little magnetic particle is produced by magnetic means and fall to container bottom; Fig. 2 is a switch in opening following time, and sampling probe is taked little magnetic particle of container bottom; Fig. 3 shows is moving magnet when placing container bottom, the magnetic attraction that little magnetic particle is produced by magnetic means and fall to container bottom; Fig. 4 shows is moving magnet when moving apart container bottom, and sampling probe is taked little magnetic particle of container bottom.In the above accompanying drawing, 1: sampling probe; 2: the container that little magnetic particle is housed; 3, the little magnetic particle of container bottom that little magnetic particle is housed is concentrated even distributive province; 4: ultrasonic detector, 5: the magnetic means of gate-controlled switch.
Five, specific embodiment
Before sampling probe is taked little magnetic particle; The gate-controlled switch of magnetic means is opened (or move to container bottom with magnet); Little magnetic particle is concentrated in the conical base of container; Close gate-controlled switch (or move apart container bottom with magnet), the syringe needle of then taking a sample is assigned the container bottom sampling and is goed deep into little magnetic particle and concentrate and draw little magnetic particle in the homogeneity range.The ultrasound wave of container side or photoelectric detector are through the decline at interface on the little magnetic particle of monitoring, to judge the quantity or the uniformity coefficient of the little magnetic particle of residue.
Claims (3)
1. method that the little magnetic particle that is applicable to fully-automatic analyzer is accurately taked; It is characterized in that: before taking little magnetic particle; The magnetic means that little magnetic container for granule bottom is housed is opened; Make little magnetic particles settling be concentrated to container bottom and form local uniform to distribute, then close magnetic means, sampling probe is visited to container bottom and is drawn little magnetic particle subsequently.
2. the method that a kind of little magnetic particle that is applicable to fully-automatic analyzer according to claim 1 is accurately taked; It is characterized in that: its magnetic means that is arranged at container bottom can come the generation of controlling magnetic field through gate-controlled switch or moving magnet as required or close, and accurately quantitatively takes with convenient in the even distribution of container bottom thereby control little magnetic particle.
3. the method that a kind of little magnetic particle that is applicable to fully-automatic analyzer according to claim 1 is accurately taked; It is characterized in that: a ultrasound wave or photoelectric detector can be installed at the container side; The decline that this detecting device passes through to detect interface on little magnetic particle is to judge the quantity or the uniformity coefficient of the little magnetic particle of residue in the container; The interface drops to a certain degree on little magnetic particle, then stops to take little magnetic particle, thereby guarantees little magnetic grain amount and needed basically identical that sampling probe is got.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105489478A CN102466732A (en) | 2010-11-18 | 2010-11-18 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105489478A CN102466732A (en) | 2010-11-18 | 2010-11-18 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102466732A true CN102466732A (en) | 2012-05-23 |
Family
ID=46070635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105489478A Pending CN102466732A (en) | 2010-11-18 | 2010-11-18 | Method for precisely absorbing micro magnetic particle with automatic analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102466732A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797093A (en) * | 2019-03-11 | 2019-05-24 | 中国科学院苏州生物医学工程技术研究所 | A kind of consumptive material, device and method for extracting nucleic acid for nucleic acid extraction |
| CN115254834A (en) * | 2022-08-02 | 2022-11-01 | 宋世琦 | Magnetic particle cleaning device and cleaning method for immunoassay device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1986006493A1 (en) * | 1985-04-29 | 1986-11-06 | Labsystems Oy | Method and device for carrying out immunological assays |
| CN1295669A (en) * | 1998-02-06 | 2001-05-16 | 生物磁性有限公司 | Device and system for transfer of material |
| US6706536B1 (en) * | 2000-11-28 | 2004-03-16 | Wada, Inc. | Method and apparatus for processing coagulation studies, chemistry procedures and the like |
| CN101273258A (en) * | 2005-09-30 | 2008-09-24 | 卡钳生命科学股份有限公司 | Microfluidic device for purifying a biological component using magnetic beads |
| CN101539583A (en) * | 2008-03-17 | 2009-09-23 | 株式会社日立高新技术 | Automatic analyzer |
-
2010
- 2010-11-18 CN CN2010105489478A patent/CN102466732A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1986006493A1 (en) * | 1985-04-29 | 1986-11-06 | Labsystems Oy | Method and device for carrying out immunological assays |
| CN1295669A (en) * | 1998-02-06 | 2001-05-16 | 生物磁性有限公司 | Device and system for transfer of material |
| US6706536B1 (en) * | 2000-11-28 | 2004-03-16 | Wada, Inc. | Method and apparatus for processing coagulation studies, chemistry procedures and the like |
| CN101273258A (en) * | 2005-09-30 | 2008-09-24 | 卡钳生命科学股份有限公司 | Microfluidic device for purifying a biological component using magnetic beads |
| CN101539583A (en) * | 2008-03-17 | 2009-09-23 | 株式会社日立高新技术 | Automatic analyzer |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797093A (en) * | 2019-03-11 | 2019-05-24 | 中国科学院苏州生物医学工程技术研究所 | A kind of consumptive material, device and method for extracting nucleic acid for nucleic acid extraction |
| CN109797093B (en) * | 2019-03-11 | 2023-09-29 | 中国科学院苏州生物医学工程技术研究所 | Consumable, device and nucleic acid extraction method for nucleic acid extraction |
| CN115254834A (en) * | 2022-08-02 | 2022-11-01 | 宋世琦 | Magnetic particle cleaning device and cleaning method for immunoassay device |
| CN115254834B (en) * | 2022-08-02 | 2023-10-27 | 宋世琦 | Magnetic particle cleaning device and method for immunoassay equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203954732U (en) | A kind of Multi-channel solid phase extraction device | |
| EP1746423A3 (en) | Automatic analysis apparatus and dispensing method for the same | |
| McKelvey et al. | Resistive pulse delivery of single nanoparticles to electrochemical interfaces | |
| Zhang et al. | Metabolism-based capture and analysis of circulating tumor cells in an open space | |
| CN204078781U (en) | A kind of asymmetric vessel array device | |
| CN102466732A (en) | Method for precisely absorbing micro magnetic particle with automatic analyzer | |
| CN106457248B (en) | Device, method and use for transferring liquids | |
| JP2013156256A (en) | Method for heating liquid of constant volume in heated pipette needle part | |
| CN205384283U (en) | Immunofluorescence analyzer | |
| CN102262082B (en) | Optical fiber sensor-molecular imprinting online detection method for detecting propofol in biological samples and optical fiber sensor-molecular imprinting online detection device. | |
| Ascione et al. | Investigation of cell dynamics in vitro by time lapse microscopy and image analysis | |
| CN209069778U (en) | Microfluidizer nano particle size on-line measuring device | |
| CN104745468A (en) | Micro-sized target hybrid electro-kinetic control method based on micro-fluidic chip | |
| CN210207236U (en) | Multi-channel ultra-high speed centrifugal layering liquid separation device | |
| CN207036542U (en) | A kind of mixed four separating devices of pedotheque | |
| CN204228503U (en) | A kind of control type dry type Nitrogen evaporator | |
| CN202133580U (en) | Heating mechanism for fully automatic glycosylated hemoglobin analyzer | |
| Antaw et al. | On the Behavior of Nanoparticles beyond the Nanopore Interface | |
| CN110261595A (en) | Sample liquid suitable for chemical illumination immunity analysis instrument dilutes mixing method | |
| CN204714791U (en) | Clinical laboratory's dilution instrument | |
| CN202570202U (en) | Splash-proof multi-port reagent bottle | |
| CN207336555U (en) | A kind of crosspointer sample introduction arm component of autosampler | |
| CN207407928U (en) | A kind of macromolecule color environmental protection stitches colloidal materials detection device | |
| CN105223371A (en) | A kind of bench top flow cytometer sheath fluid device for casting | |
| CN204255740U (en) | A kind of Nitrogen evaporator controlling flow |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120523 |