JP2013217882A - Reagent stirring mechanism and autoanalyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To attain equalization of the content of magnetic particles in a reagent containing the magnetic particles, and reduction of contamination in the reagent in an autoanalyzer which analyzes a specimen by using the reagent.SOLUTION: An autoanalyzer includes a mechanism for stirring a solution containing magnetic particles in a reagent pack 105, and has: a reagent disk (106b) on which the reagent pack 105 is loaded; a stirring bar 202 which is loaded in the reagent pack 105; a stirring mechanism 110 for operating the stirring bar; and a reagent probe 109 which sucks the solution. The reagent probe 109 and the stirring bar 202 are constituted so as not to interfere each other. Then, a stirring operation is performed by rotating the stirring bar 202 in conjunction with a rotation operation of the stirring mechanism 110, and a suction operation by the reagent probe 109 is performed during the stirring operation.

Description

  The present invention relates to a reagent stirring mechanism and an automatic analyzer, for example, a technique useful when applied to a stirring mechanism that stirs a solution containing magnetic particles and the like, and an automatic analyzer that includes the stirring mechanism.

  The automatic analyzer identifies the concentration of biological components contained in blood, urine and the like, and can analyze many samples with high reproducibility in a short time. In this automatic analyzer, immunoassay is used to determine whether an item requiring high-sensitivity measurement or a specific antigen or antibody exists. In the immunoassay, the concentration of the target substance is measured by capturing the target substance using an antibody labeled with a luminescent substance and magnetic particles with an antibody, performing B / F separation, and then measuring the luminescence amount of the target substance. The method of calculating is generally.

  Since magnetic particles used in immunoassay have a high specific gravity in the solution and settle to the bottom of the container over time, the automatic analyzer stirs the liquid reagent containing magnetic particles at regular intervals or just before dispensing. ing. In this case, a method is generally used in which stirring is performed by immersing and rotating a stirring rod from the upper part of the reagent container. A technique relating to such stirring of magnetic particles is described in Patent Document 1, for example.

  In addition to the stirring method for rotating the stirring bar, Patent Document 2 describes a stirring method using sound waves. According to this method, as compared with the conventional stirring by rotating the stirring rod, the stirring can be performed in a non-contact manner, so that efficient mixing can be performed without causing carry-over between the objects to be stirred.

  In the immunoassay, the amount of magnetic particles attracted greatly affects the measurement result, but it is difficult to always attract the same amount of magnetic particles because the sedimentation of the magnetic particles starts from the time when the stirring is completed. A method for solving this problem is described in Patent Document 3. According to this method, since the liquid is disturbed by the reagent probe and agitation can be simultaneously performed while stirring with the reagent probe, the reagent can be always dispensed in a constant stirring state.

  Further, Patent Document 4 describes a method capable of simultaneously stirring and aspirating the reagent container. In this method, the stirrer is set inside the reagent container, and stirring is performed by rotating the stirrer by transmitting the magnetic force of the magnet rotating body. Since the obstacle which interferes with suction mechanisms, such as a stirring mechanism, does not exist in the reagent container upper part like patent document 1, aspiration can be performed at the time of stirring operation. In addition to the analyzer, Patent Document 5 describes a method of stirring a liquid reagent or a liquid sample in a container by tilting the reagent container in a horizontal direction and performing a reciprocating motion. .

JP 2010-101677 A JP 2003-35715 A JP 2009-25167 A JP 2005-169303 A JP 2002-1137 A

  However, the above stirring method has the following problems. In the analyzer described in Patent Document 1, since the same stirring rod is used to stir the magnetic particles in each reagent container, there is a possibility that contamination due to reagent carryover may occur when stirring different reagents. is there. Further, since there is a time interval of several seconds at a minimum between the stirring operation and the dispensing operation, the suction operation is performed when the magnetic particles are settled, and the amount of magnetic particles to be attracted may vary. There is.

  When the method of stirring using sound waves as in Patent Document 2 is used for stirring magnetic particles used in immunoassay, there is a possibility that chemical substances modified with magnetic particles will be separated in order to capture antigens. There is. In an immunoassay that requires high-sensitivity measurement, it is necessary to stir uniformly while having the function of capturing an antigen on magnetic particles.

  In the method of stirring the solution using the reagent probe as in Patent Document 3, suction is possible during stirring of the reagent, but compared with Patent Document 1, the stirring efficiency is low, and the specific gravity is particularly low like magnetic particles. It takes a lot of time to uniformly stir heavy substances into the solution. Further, in Patent Document 3, the liquid reagent is disturbed by three-dimensional position movement, but the probe is immersed in the reagent more than necessary, so that the contamination range by the reagent may be widened. There is. It takes a lot of time to clean this contaminated part, and the risk of carryover of the reagent increases.

  When the method of Patent Document 4 is used for stirring the reagent for immunoassay, the magnetic particles are attracted to the magnet rotating body, making it difficult to stir uniformly. Moreover, the method of vibrating the container itself as in Patent Document 5 is low in stirring efficiency because the vibration for stirring is either horizontal or vertical reciprocating, and stirring of a substance having a high specific gravity such as magnetic particles. It is unsuitable for.

  The present invention has been made in view of the above, and one of its purposes is the inclusion of magnetic particles in a reagent in an automatic analyzer that analyzes a sample using a reagent containing magnetic particles. The purpose is to achieve uniform amount and reduction of contamination in the reagent. The above and other objects and novel features of the present invention will be apparent from the description of this specification and the accompanying drawings.

  Of the inventions disclosed in the present application, the outline of a typical embodiment will be briefly described as follows.

  The automatic analyzer according to the present embodiment includes a mechanism for stirring a solution containing magnetic particles in a reagent container, a mounting board on which the reagent container is mounted, a stirrer mounted in the reagent container, and the stirrer And a probe for sucking the solution in the reagent container, and the probe and the stirrer do not interfere with each other. Then, the solution in the reagent container is stirred by rotating the stirring bar in conjunction with the rotation operation of the stirring mechanism, and the suction operation by the probe is performed during the stirring operation.

  According to the embodiment, when analyzing a specimen using a reagent containing magnetic particles, the content of magnetic particles in the reagent can be made uniform and contamination in the reagent can be reduced.

1 is a system configuration diagram showing an example of the overall configuration of an automatic analyzer according to Embodiment 1 of the present invention. FIG. It is sectional drawing which shows the detailed structural example around the reagent pack and stirring mechanism in FIG. It is a flowchart which shows the operation example of the automatic analyzer of FIG. 1 provided with the stirring mechanism of FIG. FIG. 4 is a cross-sectional view showing a detailed configuration example around a reagent pack and a stirring mechanism in FIG. 1 in an automatic analyzer according to Embodiment 2 of the present invention.

  In the following embodiment, when it is necessary for the sake of convenience, the description will be divided into a plurality of sections or embodiments. However, unless otherwise specified, they are not irrelevant, and one is the other. Some or all of the modifications, details, supplementary explanations, and the like are related. Further, in the following embodiments, when referring to the number of elements (including the number, numerical value, quantity, range, etc.), especially when clearly indicated and when clearly limited to a specific number in principle, etc. Except, it is not limited to the specific number, and may be more or less than the specific number.

  Further, in the following embodiments, the constituent elements (including element steps and the like) are not necessarily indispensable unless otherwise specified and apparently essential in principle. Needless to say. Similarly, in the following embodiments, when referring to the shapes, positional relationships, etc. of the components, etc., the shapes are substantially the same unless otherwise specified, or otherwise apparent in principle. And the like are included. The same applies to the above numerical values and ranges.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. Note that components having the same function are denoted by the same reference symbols throughout the drawings for describing the embodiment, and the repetitive description thereof will be omitted.

(Embodiment 1)
<< Overall configuration and operation of automatic analyzer >>
FIG. 1 is a system configuration diagram showing an example of the overall configuration of an automatic analyzer according to Embodiment 1 of the present invention. The automatic analyzer shown in FIG. 1 is an immune analyzer that analyzes the concentration of biological components contained in blood, urine, and the like. 1 includes a reagent disk (mounting board) 106 on which a reagent pack (reagent container) 105 is mounted, a reaction disk (mounting board) 118 on which a reaction container 107b is mounted, and a sample (or sample or specimen). A rack 111 on which the sample container 101 containing the sample container 101 is loaded, a chip buffer unit 115, a disposal hole 116, and a detection unit 119.

  In addition, the automatic analyzer of FIG. 1 has, as a transport mechanism, a chip / reaction container transport mechanism 114 for transporting the reaction container 107a and the chip 113 to the reaction disk 118 and the chip buffer unit 115, respectively, and a rack 111 at a predetermined position. A rack transport mechanism 112 is provided. Furthermore, as a suction or dispensing mechanism, a reagent probe 109 for dispensing the reagent in the reagent pack 105, a sample probe 108 for dispensing the sample in the sample container 101, and a reaction for sucking the reaction solution in the reaction container 107b A solution suction nozzle 117 is provided. Here, the probe means a dispensing means capable of sucking and discharging a target liquid by a pressure generating means such as a syringe. The part directly touched by the liquid to be dispensed may be referred to as a nozzle, but the probe includes the nozzle as one of the components, and is used to include an arm for moving the nozzle to a target position. In some cases.

  The reagent pack (reagent container) 105 is configured by integrating a bead bottle 102, a first reagent bottle 103, and a second reagent bottle 104. The bead bottle 102 contains a bead solution (reagent) containing magnetic particles, the first reagent bottle 103 contains a first reagent for binding the magnetic particles to a specific component in the sample, and a second reagent. The bottle 104 contains a second reagent for labeling a labeling substance that emits light by an electrochemical reaction and binding it to a specific component in the sample. The reagent pack 105 sequentially moves by the rotation control of the reagent disk 106 and reaches the stirring mechanism 110 provided at a specific location on the reagent disk 106. At this time, as will be described in detail later, a system in which the bead solution (reagent) in the bead bottle 102 is stirred and dispensed using the reagent probe 109 is one of the main features of the first embodiment. Yes.

  Next, an overall operation example of the automatic analyzer of FIG. 1 will be described. The sample container 101 is mounted on the rack 111 and transported to the sample suction position by the rack transport mechanism 112. In order to prevent contamination between samples, the sample probe 108 attaches the tip 113 to the tip of the probe and then dispenses the sample to the reaction vessel 107b. That is, the sample probe 108 is attached with the tip 113 carried to the tip buffer unit 115 by the tip / reaction container transport mechanism 114 before dispensing, and the used tip 113 is placed in the disposal hole 116 after dispensing. Discard.

  A plurality of reagent packs 105 can be loaded on the reagent disk 106, and these reagent packs 105 can be moved to a reagent dispensing / stirring position on the circumference by rotating the disk. On the other hand, the chip / reaction container transport mechanism 114 transports the reaction container 107a onto the reaction disk 118 before dispensing of the sample and the reagent. The reaction disk 118 can appropriately move the conveyed reaction vessel 107b to the sample dispensing position and the reagent dispensing position on the circumference by controlling the rotation of the disk. In response to this, the sample probe 108 dispenses the sample into the reaction vessel 107b with the chip 113 mounted as described above, and the reagent probe 109 dispenses the reagent into the reaction vessel 107b.

  The reaction disk 118 is maintained at around 37 ° C., which is the optimum temperature for the antigen-antibody reaction. After the sample / reagent is dispensed into the reaction vessel 107 b and reacted on the reaction disk 118 for a certain period of time, the reaction solution suction nozzle 117 sends the reaction solution in the reaction vessel 107 b to the detection unit 119. The detector 119 measures the luminescence value of the reaction solution. The measured reaction container 107b is transported to the upper part of the disposal hole 116 by the chip / reaction container transport mechanism 114 and discarded.

  Here, the sample in the sample container 101 is a sample derived from a biological fluid such as serum or urine. The bead solution (reagent) in the bead bottle 102 is obtained by, for example, dispersing avidin-labeled magnetic particles in a buffer solution in order to bind to the first reagent. The first reagent in the first reagent bottle 103 is, for example, a biotin-labeled antibody, and becomes a complex of the antibody and magnetic particles by avidin and biotin-avidin bonds bound to the magnetic particles. Here, an antibody that specifically binds to the substance to be measured is used. The second reagent in the second reagent bottle 104 is an antibody labeled with a luminescent substance, and specifically reacts with a measurement target substance captured by a complex of magnetic particles and an antibody or an antibody reacted with the measurement target substance.

  In such a configuration and operation, the magnetic particles in the bead bottle 102 settle out over time, and therefore a device for making the content of the magnetic particles contained in the dispensed bead solution (reagent) uniform. Needed. It is also important to prevent contamination from mixing in the bead solution (reagent). Therefore, it is beneficial to use the stirring and dispensing method of the first embodiment described below.

<< Details around reagent pack and stirring mechanism >>
FIG. 2 is a cross-sectional view showing a detailed configuration example around the reagent pack and the stirring mechanism in FIG. The reagent pack 105 shown in FIG. 2 has a configuration in which three reagent containers (bead bottle 102, first reagent bottle 103, and second reagent bottle 104) are connected. On the bottom surface of the bead bottle 102, there are provided a rotatable uneven portion (connecting portion) 201 and a stirring bar 202 that is integrally formed with the uneven portion 201 and rotates in conjunction with the uneven portion 201. That is, the stirrer 202 is rotated by rotating the concavo-convex portion 201, and the bead solution (reagent) can be stirred.

  The stirring mechanism 110 is installed in the lower part of the reagent pack 105 transported to the dispensing / stirring position, and includes a motor (drive mechanism) 203 for rotating the stirrer 202 and an uneven portion on the bottom surface of the bead bottle 102 ( A concavo-convex portion (connecting portion) 204 connected to the connecting portion 201, a beam sensor 205, and a lifting mechanism 206 are provided. The reagent disk 106 is not particularly limited, but here is constituted by a bowl-shaped reagent disk tank 106a and a dish-shaped reagent disk body (mounting board) 106b. The reagent disk main body (mounting board) 106b is provided inside the reagent disk tank 106a, and rotates as the reagent pack 105 is loaded as described in FIG. 1 (with the vertical line on the disk surface as the axis). Rotation operation). The concavo-convex portion (connecting portion) 201 and the concavo-convex portion (connecting portion) 204 have a shape that physically fits in the opposing portions of the disk surface in the vertical direction. The raising / lowering mechanism 206 has a function of raising and lowering the concavo-convex portion (connecting portion) 204 and the like in the vertical direction of the disk surface, and constitutes an attaching / detaching mechanism including the concavo-convex portions (connecting portions) 201 and 204.

  A part of the bottom surface of the reagent disk tank 106a is provided with a cylinder (opening) 207 for moving up and down and rotating the concavo-convex part 204 and the like disposed in the reagent disk tank 106a. The cylinder 207 is formed as small as possible so as not to change the internal temperature of the reagent disk tank 106a. The reagent disk main body 106 b is formed with an opening 210 for connecting the concavo-convex portion 201 to the concavo-convex portion 204 at the bottom surface portion of the bead bottle 102. Further, the reagent disk tank 106a is provided with a bottle holding unit 208 so that the reagent pack 105 is not lifted by a force from below when connected by the stirring mechanism 110.

  The stirrer 202 has a structure that does not exist at the center of the bead bottle 102 so that it does not interfere with the reagent probe 109 even if it is rotated. That is, the stirrer 202 is installed on the bottom side of the bead bottle 102, and the reagent probe 109 is inserted from the ceiling side opposite to the stirrer 202, and there is a sufficient distance between the stirrer 202 and the reagent probe 109. It is secured. This makes it possible to perform suction (dispensing) by the reagent probe 109 during the stirring operation by the stirring bar 202. However, if the reagent liquid level in the bead bottle 102 is lowered by continuing to use the bead solution (reagent), the solution moves to the wall surface of the container due to the stirring operation, and for example, air is sucked during suction. The reagent may not be able to be aspirated. Therefore, in the first embodiment, control is performed to slow down the rotation speed of the stirrer 202 as the reagent remaining amount decreases. Further, when the remaining amount of the reagent becomes a very small amount, the stirring time is shortened and control is performed so that the stirring operation is not performed during the suction.

  The beam sensor 205 includes a beam sensor light projecting unit and a beam sensor light receiving unit disposed at a position facing the beam sensor projecting unit, and exists to confirm that the uneven portions (201, 204) are connected to each other. When connected, the light emitted from the beam sensor light projecting unit passes through the optical path hole 209 for the beam sensor existing inside the uneven portion 201, and the beam sensor light receiving unit detects this. When the beam sensor light-receiving unit detects light (when connection is successful), the stirring mechanism 110 starts the stirring operation by rotating the stirring bar 202. On the other hand, when the beam sensor light receiving unit does not detect light (when connection is unsuccessful), the stirring mechanism 110 generates an alarm to notify the operator of the abnormality, for example.

  During the rotation operation of the reagent disk main body 106b, the concavo-convex part (connecting part) 204 and the beam sensor 205 are at the lowering point, and the reagent disk main body 106b and the stirring mechanism 110 are not in interference. When the bead bottle 102 reaches the stirring position through the rotation operation of the reagent disk main body 106b, the stirring mechanism 110 raises the concavo-convex part (connecting part) 204 and the beam sensor 205, and the concavo-convex part (201, 204) is moved between After connecting, the stirring operation is started. When the agitation / dispensing operation is completed, the agitation mechanism 110 lowers the concavo-convex portion (connecting portion) 204 and the beam sensor 205 to the lowering point and returns them to a position where they do not interfere with the reagent disk main body 106b.

  3 is a flowchart showing an operation example of the automatic analyzer of FIG. 1 having the stirring mechanism of FIG. First, although not shown, the reaction container 107b is conveyed onto the reaction disk 118 by the chip / reaction container conveyance mechanism 114, and the sample is dispensed into the reaction container 107b by the sample probe. Next, the reagent disk 106 rotates, and the reagent pack (reagent container) 105 corresponding to the item to be analyzed moves to the dispensing / stirring position (step S301). After the rotation of the reagent disk 106 stops, the reagent probe 109 dispenses the first and second reagents into the reaction vessel 107b into which the sample has been dispensed.

  At the time of this dispensing, the stirring mechanism 110 first raises the concavo-convex portion 204 and the like while rotating at a low speed, and uses the beam sensor 205 to check whether the concavo-convex portion 204 is normally connected to the concavo-convex portion 201. (Step S302). If the connection is not normally performed, an alarm is generated (step S308). On the other hand, when the connection is normally performed, the remaining amount of the reagent is confirmed (step S303). The reagent remaining amount is separately managed by a mechanism not shown in FIGS. The agitation mechanism 110 refers to the reagent remaining amount, determines the motor rotation number corresponding to the reagent, rotates the motor 203, and agitates the bead solution (reagent) through the rotation of the agitation element 202.

  At this time, for example, when the reagent remaining amount is equal to or higher than the reference value (Aml), the stirring mechanism 110 uses a high-speed motor rotation number (step S304), and the reference value (Bml) is smaller than the reference value (Aml). ) In the above case, a low-speed motor rotation speed is used (steps S309 and S310). Then, the reagent probe 109 descends with the stirring bar 202 rotating at this high speed or low speed, and aspirates the bead solution (reagent) (step S305). Thereafter, the reagent probe 109 is raised and the stirring operation is stopped (step S306). The reagent probe 109 dispenses the aspirated reagent into the reaction vessel 107b on the reaction disk 118, and the stirring mechanism 110 moves up and down. The mechanism 206 is used to release the connection between the concave and convex portions (201, 204) (step S307).

  On the other hand, when the reagent remaining amount is smaller than the reference value (Bml), the stirring mechanism 110 stops stirring after stirring using the low-speed motor rotation number (steps S309, S311, and S312). Then, the reagent probe 109 descends while the rotation of the stirring bar 202 is stopped, and aspirates the bead solution (reagent) (step S313). Thereafter, the reagent probe 109 rises and then turns to the reaction disk 118 side, dispenses the reagent sucked into the reaction vessel 107b (step S314), and the agitation mechanism 110 uses the lifting mechanism 206 to move the uneven portion. The connection between (201, 204) is released (step S307).

  Thereafter, the reagent disk 106 starts rotating again, and returns to step S301 to repeat the same operation. Note that the dispensing timing of the bead solution varies depending on the item to be measured. If the bead solution is to be dispensed immediately after dispensing the first and second reagents, before (or in parallel with) the stirring operation in steps S304, S310, and S311 of FIG. The probe 109 performs suction and dispensing into the reaction container 107b for each of the first reagent and the second reagent.

  As described above, by using the reagent stirring mechanism and the automatic analyzer according to the first embodiment, for example, the following effects can be obtained. First, by installing the stirrer and the probe on opposite sides of the reagent container, it is possible to perform the stirring and dispensing operations at the same time. The stirring state of the magnetic particles during reagent aspiration (dispensing) is constant. Can be. In other words, the content of magnetic particles contained in the aspirated solution can be kept almost uniform, and the measurement conditions for each sample are made uniform, so the reliability (reproducibility) of the measurement results can be improved. become. In addition, by installing a stirrer in the reagent container, it is not necessary to use the same stirrer between reagents, and other reagents and cleaning water (ie, contamination) adhering to the stirrer are brought in (carry over) ) Can be prevented. This also makes it possible to improve the reliability (reproducibility) of measurement results. Furthermore, the time required for analysis of each specimen can be shortened because the stirrer that has been conventionally required is not required to be cleaned and the stirring operation and the dispensing operation can be performed simultaneously.

  In FIG. 2, the stirrer 202 has a shape having a plurality of blades extending in an oblique direction. However, the shape is not limited to this, and the shape may be changed as appropriate so as to increase the stirring efficiency. Is possible. For example, it is also possible to use a propeller-like shape or a shape like a so-called washing machine pulsator. However, in order not to interfere with the probe, it is desirable to use a shape having a relatively low height. Further, here, the connection is determined using the beam sensor 205. However, for example, it is possible to perform the determination using a contact sensor or depending on the operation amount of the lifting mechanism 206 in some cases.

(Embodiment 2)
<< Details of Reagent Pack and Stirring Mechanism (Modification) >>
FIG. 4 is a sectional view showing a detailed configuration example around the reagent pack and the stirring mechanism in FIG. 1 in the automatic analyzer according to the second embodiment of the present invention. The reagent pack (reagent container) 105a shown in FIG. 4 includes, in addition to the three bottles (102, 103, 104), the stirrer 202 and the same at the bottom of the bead bottle 102, as in FIG. And a concavo-convex portion (connecting portion) 301 configured integrally. However, here, the optical path hole 209 is not provided in the uneven portion (connecting portion) 301, and the uneven portion (connecting portion) 301 has a gear-like shape having a plurality of teeth.

  4 includes a concavo-convex portion (connecting portion) 302 and a motor (driving mechanism) 203, as in the case of FIG. However, here, the beam sensor 205 and the lifting mechanism 206 are not provided in the stirring mechanism 110a. Unlike the case of FIG. 2, the concavo-convex portion (connecting portion) 302 and the concavo-convex portion (connecting portion) 301 have a shape that physically engages (fits) at a portion facing in the horizontal direction of the reagent disk surface. . Here, the uneven portions (connecting portions) 301 and 302 have a gear-like shape having a plurality of teeth, and are connected in the horizontal direction of the reagent disk surface. Accordingly, it is not necessary to provide the opening 210 in the reagent disk main body 106b as shown in FIG. 2, and the cylinder (opening) 207 of the reagent disk tank 106a is located at a position different from that in FIG. Provided.

  In such a configuration example, the reagent pack 105a is moved to the stirring / dispensing position by the rotation of the reagent disk 106 (reagent disk main body 106b), so that the uneven part 301 on the bottom surface of the bead bottle 102 has an uneven part on its side surface. 302 is connected. And in this connected state, the stirring mechanism 110a rotates the stirrer 202 by rotating the motor 203 to perform the stirring operation of the magnetic particles. In this configuration example, the concavo-convex portion 301 and the concavo-convex portion 302 are attached / detached (connected / detached) each time each reagent pack 105a passes through the installation location of the stirring mechanism 110a as the reagent disk 106 rotates. Since the pair of gears (301, 302) are both freely rotatable, there is no particular hindrance to the rotation operation of the reagent disk 106.

  Even if the reagent stirring mechanism and the automatic analyzer according to the second embodiment are used, the same effect as in the first embodiment can be obtained. Furthermore, compared to the case of the first embodiment, the need for the lifting / lowering operation by the stirring mechanism for each stirring operation is eliminated, so the configuration of the stirring mechanism can be simplified, and the time required for analysis of each specimen can be reduced. It can be shortened. However, the tolerance for the relative displacement between the uneven portion (connecting portion) 301 and the uneven portion (connecting portion) 302, and the wear degree (durability) of the uneven portion (connecting portion) 301 and the uneven portion (connecting portion) 302. 2), the configuration example of FIG. 2 is considered more desirable.

  The main configuration obtained by this embodiment is summarized as follows.

  (1) The automatic analyzer according to the present embodiment includes a reagent container in which a solution containing magnetic particles is placed, a mounting board on which the reagent container is mounted, and a solution installed by rotating inside the reagent container. A stirrer that performs agitation, a detachment mechanism that physically attaches and detaches the stirrer from the outside of the reagent container, a drive mechanism that controls the rotation operation of the stirrer via the attachment and detachment mechanism, A probe for aspirating the solution from the inside of the reagent container, and the probe aspirates the solution while the drive mechanism rotates the stirrer via the attachment / detachment mechanism.

  (2) In the automatic analyzer of (1), the stirrer is installed on the mounting board side of the reagent container, and the probe is from the side facing the stirrer in the vertical direction of the mounting board. Aspirate the solution.

  (3) In the automatic analyzer according to (2), the attachment / detachment mechanism includes a first connection part coupled to the stirrer, a second connection part coupled to the drive mechanism, and the second connection part. An elevating mechanism that moves in the vertical direction, and the mounting board is configured to be able to rotate around the vertical direction of the mounting board, and the first connection part and the second connection part are in the vertical direction. In which the opposing parts are fitted.

  (4) In the automatic analyzer according to (2), the attachment / detachment mechanism includes a third connection part coupled to the stirrer and a fourth connection part coupled to the drive mechanism, and the mounting board includes The mounting board is configured to be rotatable about the vertical direction of the mounting board, and the third connecting part and the fourth connecting part have a shape in which portions facing each other in the horizontal direction of the mounting board are fitted.

  (5) In the automatic analyzer of (2), the drive mechanism variably controls the rotation speed of the stirrer according to the amount of the solution in the reagent container.

  (6) In the automatic analyzer according to (5), when the amount of the solution in the reagent container is smaller than a predetermined amount, the probe is moved by the drive mechanism via the attachment / detachment mechanism. Then, the solution is sucked in a state where the rotation operation is stopped.

  (7) Further, the automatic analyzer according to the present embodiment has a mechanism for stirring a solution containing magnetic particles in a reagent container, and includes a mounting board on which the reagent container is mounted, The stirrer mounted on the agitator, a stirrer mechanism for operating the stirrer, and a probe for sucking the solution, the probe and the stirrer have a structure that does not interfere with each other. The solution is agitated by rotating the stirrer in conjunction with the operation, and the solution is aspirated using the probe during the agitation of the solution.

  (8) In the automatic analyzer according to (7), the stirring mechanism includes a mechanism that is connected to the stirrer at the time of the stirring operation and is detached from the stirrer at the end of stirring, and when the mounting board moves. The mounting board and the stirring mechanism do not interfere with each other.

  (9) In the automatic analyzer according to (7), the automatic analyzer does not include a stirrer for stirring the solution in the reagent container other than the stirrer, and further does not include a cleaning mechanism for the stirrer. .

  (10) In addition, the reagent stirring mechanism according to the present embodiment includes a reagent container in which a solution containing magnetic particles is placed, a stirrer that is installed inside the reagent container and stirs the solution by a rotating operation, In order to physically attach and detach the stirrer from the outside of the reagent container, the connecting mechanism includes a portion that is coupled to the stirrer and is exposed to the outside of the reagent container.

  (11) In the reagent stirring mechanism of (10), the stirring bar is installed at the bottom of the reagent container.

  As mentioned above, the invention made by the present inventor has been specifically described based on the embodiments. However, the present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the scope of the invention.

DESCRIPTION OF SYMBOLS 101 Sample container 102 Bead bottle 103 1st reagent bottle 104 2nd reagent bottle 105,105a Reagent pack 106 Reagent disk 106a Reagent disk tank 106b Reagent disk main body 107a, 107b Reaction container 108 Sample probe 109 Reagent probe 110, 110a Agitation mechanism 111 Rack 112 Rack transport mechanism 113 Chip 114 Chip / reaction container transport mechanism 115 Chip buffer section 116 Disposal hole 117 Reaction solution suction nozzle 118 Reaction disk 119 Detection section 201, 204 Concavity and convexity 202 Stirring bar 203 Motor 205 Beam sensor 206 Lifting mechanism 207 Tube 208 Bottle holding part 209 Optical path hole 210 Opening part 301,302 Uneven part

Claims (5)

A reagent container in which a solution containing magnetic particles is placed;
A mounting board on which the reagent container is mounted;
A stirring bar installed inside the reagent container and stirring the solution by a rotating operation;
An attachment / detachment mechanism for physically attaching / detaching the stirrer from the outside of the reagent container;
A drive mechanism for controlling the rotation of the stirrer via the attachment / detachment mechanism;
A probe for aspirating the solution from the reagent container;
The probe is an automatic analyzer that sucks the solution in a state where the drive mechanism rotates the stirrer via the attachment / detachment mechanism. The automatic analyzer according to claim 1, wherein
The stirring bar is installed on the mounting board side of the reagent container,
The said probe is an automatic analyzer which attracts | sucks the said solution from the side facing the said stirring element in the perpendicular direction of the said mounting board. The automatic analyzer according to claim 2,
The automatic analyzer is configured to variably control the number of rotations of the stirrer according to the amount of the solution in the reagent container. A reagent container in which a solution containing magnetic particles is placed;
A stirring bar installed inside the reagent container and stirring the solution by a rotating operation;
A reagent agitation mechanism having a coupling mechanism that includes a portion that is coupled to the agitation element and exposed to the outside of the reagent container in order to physically attach and detach the agitation element from the outside of the reagent container. The reagent stirring mechanism according to claim 4,
The stirring bar is a reagent stirring mechanism installed at the bottom of the reagent container.

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