JP2010101677A - Stirring machine of magnetic particle solution - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a stirring machine of a magnetic particle solution capable of uniformly and efficiently dispersing magnetic particles contained in a solution. <P>SOLUTION: In the stirring machine of the magnetic particle solution equipped with a stirring member for mixing a solution containing magnetic particles in a magnetic particle solution container by stirring, the stirring member has: a stirring rotary shaft and the stirring blades which is large in extension in a radial direction; and stirring blades which is small in extension in the same direction provided at the stirring rotary shaft. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a magnetic particle solution stirring device for efficiently improving the dispersibility of a solution containing magnetic particles in immunoassay or genetic analysis using magnetic particles as a solid phase.

  Analysis methods using magnetic particles are widely used in the medical field, biotechnology field, and the like. Among these, for example, in the medical field, immunoassay using blood or the like as a sample can be mentioned.

  Immunoassay is a method for measuring a target substance with high sensitivity by immunological specific binding. RIA (radioimmunoassay) using a radioisotope as a labeling substance, EIA (enzyme immunoassay) using an enzyme, chemiluminescence There is ECLIA (electrochemiluminescence immunoassay) using a substance. In these analyzes, in order to detect a very small amount of a target substance with high sensitivity, an antigen or antibody having specific affinity is immobilized on the surface of a magnetic particle, and the target substance is immobilized by immunological specific binding. In general, the substance is detected after being bound to a phase and selectively magnetically separated and concentrated from a liquid phase containing a substance other than the target substance.

  For example, there is gene analysis in the biotechnology field. Gene analysis is a method that utilizes the property that a gene specifically binds to silica in the presence of a chaotropic reagent such as guanidine, and magnetic particles coated with silica on the surface are put into a solution containing the gene and the chaotropic reagent. In this method, the gene is isolated and purified by binding the gene to the surface of the magnetic particle.

  In these analysis methods, the magnetic particle solution having a uniform particle number and particle diameter that eliminates the spontaneous sedimentation and self-aggregation of the magnetic particles is introduced into the reaction system. It is extremely important to improve and obtain highly accurate analysis results. Therefore, a general chemical analyzer using magnetic particles has a function of stirring the magnetic particle solution with a stirring rod or the like immediately before the magnetic particle solution is put into the reaction system. The present invention relates to an improvement in a magnetic particle solution stirring apparatus that provides this stirring function.

Japanese National Patent Publication No. 10-504895 International Publication No. W097 / 31105 JP 2008-2959 A JP 2007-271411 A

  As a contact-type stirring method for eliminating sedimentation and aggregation of magnetic particles, a stirring rod equipped with a stirring bar such as a screw type, paddle type, or turbine type is inserted into the magnetic particle solution, and this is rotated to rotate the solution. The thing related to the stirring apparatus of the magnetic particle solution to stir is described in, for example, JP-T-10-504895, International Publication No. W097 / 31105, JP-A-2008-2959, JP-A-2007-271411. Has been.

  However, in this method, (1) shortening the stirring time for improving the analysis throughput, (2) reducing the amount of magnetic particle solution adhering to the stirring body, and reducing the amount of cleaning liquid brought into the solution containing magnetic particles. Reduced surface area of stirrer, (3) Reduced length of stirrer (reduced size) with reduced diameter of solution container opening to reduce evaporation and concentration of magnetic particle solution and reduce contamination risk from outside environment It is difficult to maintain sufficient stirring performance that can eliminate sedimentation and agglomeration of magnetic particles under the constraints such as.

  That is, it is required to improve the stirring efficiency of a solution containing magnetic particles while maintaining the surface area and the long diameter (large size) of the stirring body.

  In view of the above problems, an object of the present invention is to provide a magnetic particle solution stirring apparatus that can efficiently mix magnetic particles contained in a solution without unevenness.

  The present invention provides a magnetic particle solution stirring device comprising a stirring body for stirring a solution containing magnetic particles in a magnetic particle solution container, wherein the stirring body has a stirring rotation shaft and a radial extension provided on the stirring rotation shaft. It is characterized by having a large stirring blade and an extension in the same direction with a small stirring blade.

  The present invention also provides a magnetic particle solution stirring apparatus comprising a stirring body for stirring a solution containing magnetic particles in a magnetic particle solution container, the stirring body having a stirring rotating shaft and a stirring blade provided on the stirring rotating shaft. The agitation rotating shaft rotates and rotates while rotating.

  Furthermore, the present invention is characterized in that the stirring blade is provided on the lower end side of the stirring rotation shaft.

  According to the present invention, since stirring is performed over a wide range from the center to the outer periphery of the solution container with large and small stirring blades, the magnetic particles are efficiently dispersed without unevenness.

  Further, since the agitation rotating shaft rotates while rotating, the agitation blades are agitated over a wide range from the center to the outer periphery of the solution container, so that the magnetic particles can be efficiently dispersed without unevenness.

  Furthermore, since the stirring blade provided on the lower end side of the stirring rotating shaft creates a circulating flow that rotates in the vertical direction by stirring and rotating on the inner bottom side in the solution container, the magnetic particles are efficiently dispersed without any unevenness.

  Embodiments of the present invention will be described in detail below with reference to the drawings.

  First, an outline of a chemical analyzer equipped with a solution stirring apparatus according to the present invention shown in FIG. 1 will be described.

  In the chemical analyzer 100, the sample transport line 115 transports the sample 103 to a sample dispensing position in the vicinity of the sample dispensing pipetter 116.

  The sample dispensing tip / reaction vessel transport mechanism 105 is one of the sample dispensing tip / reaction vessel disposal hole 101, the sample dispensing tip buffer 102, the reaction liquid stirring mechanism 104, the sample dispensing tip / reaction vessel 106, and the incubator disk 107. Can be moved in the X, Y, and Z axis directions within the upper part of the unit, the reaction vessel from the sample dispensing tip / reaction vessel 106 to the incubator disk 107, and the sample dispensing tip to the sample dispensing tip buffer Move to 102.

  The sample dispensing pipetter 116 moves to the upper part of the sample dispensing chip buffer 102 where the sample dispensing chip is installed, attaches the sample dispensing chip, moves to the upper part of the sample, sucks the sample, and It moves to the upper part of the reaction container, discharges the sample, moves to the upper part of the sample dispensing tip / reaction container disposal hole, and discards the sample dispensing chip. The incubator disc 107 can hold a plurality of reaction vessels, and these reaction vessels are moved to predetermined positions on the circumference by a rotational motion.

  The reagent disk 112 mounts a plurality of reagent containers 111 including a magnetic particle solution container. Reagents are held in these reagent containers 111. These reagent containers 111 are moved to predetermined positions on the circumference by the rotational movement of the reagent disk 112.

  The reagent dispensing pipetter 110 moves to the upper part of the predetermined reagent container 111 in the reagent disk 112, sucks a predetermined amount of reagent from the reagent container 111, moves to the upper part of the predetermined reaction container in the incubator, and puts the reagent in the reaction container. Discharge.

  The magnetic particle stirring arm 108 (arm portion) of the horizontal and vertical movement mechanism moves to the upper part of a predetermined magnetic particle solution container in the reagent disk 112, and the magnetic particle stirring bar (stirring body) provided in the magnetic particle stirring arm 108 is lowered. Rotate to stir the magnetic particle solution in the magnetic particle solution container. The magnetic particle solution is stirred immediately before dispensing in order to eliminate spontaneous sedimentation. After the stirring, the magnetic particle stirring arm 108 moves to the upper part of the cleaning tank 109 provided with cleaning water, and the magnetic particle stirring bar is lowered and rotated to clean and remove the magnetic particle solution containing the magnetic particles adhering to the stirring bar. The magnetic particles contained in the magnetic particle solution have a particle size of 0.5 μm to 10 μm.

  The reaction solution suction nozzle 113 dispenses a sample and a predetermined reagent, sucks the reaction solution in the reaction container after a predetermined reaction time, and sends the solution to the detection unit 114. The detection unit 114 analyzes the reaction solution. The sample dispensing chip / reaction container transport mechanism 105 moves the analyzed reaction container to the upper part of the sample dispensing chip / reaction container disposal hole, and discards the sample dispensing chip.

  By combining and repeating the above operations, a plurality of items can be efficiently analyzed for a plurality of samples.

  Next, a magnetic particle solution stirring apparatus, which is the main part of the present invention, will be described with reference to FIGS.

  First, the structure of the stirring device will be described with reference to FIG.

  The stirrer 200 includes a stirrer drive unit 800 that rotates the stirrer 202 and a horizontal / vertical movement mechanism unit 900 that moves the stirrer drive unit 800 in the horizontal direction and the horizontal direction. The stirrer driving unit 800 includes a chuck 801 that holds the stirrer 202.

  Further, as shown in the schematic diagram of FIG. 6, the stirrer drive unit 800 includes a revolution drive unit 802, a rotation rotation unit 803, and a support arm 804 that supports the rotation rotation unit 803 on the revolution drive unit 802. Since the chuck 801 that holds the stirring member 202 is provided on the rotation shaft of the rotation rotating portion 803, the axis of the rotation shaft and the shaft center of the stirring member 202 are concentric.

  The revolution shaft of the revolution drive unit 802 is rotated by a revolution drive motor. The rotation shaft of the rotation rotation unit 803 is rotated by a rotation drive motor. The axis of the rotation axis and the axis of the revolution axis are separated from each other by the length of the support arm 804. The stirrer 202 rotates around the rotation axis while turning on a circular orbit away from the revolution axis by the length of the support arm 804.

  As shown in FIG. 2, the horizontal / vertical moving mechanism unit 900 includes a vertically extending pivot 901 that can be moved up and down, a vertically moving drive unit 902 that moves the pivot 901 up and down, and a pivot 901. The magnetic particle agitation arm 108 (arm part) that supports the agitator rotation drive unit 800 is provided on the end side that is provided on the upper end side of the Ax.

  The stirrer 200 causes the horizontal / vertical moving mechanism 900 to operate in synchronization with the reagent disk 112 that horizontally moves the magnetic particle container, and causes the stirrer 202 that hangs down from the tip of the magnetic particle stirring arm 108 (arm part) to move to the magnetic particle container. Move horizontally to the top of the opening. Next, the stirrer 202 is vertically lowered to insert the stirrer 202 into the magnetic particle container, and the stirrer 202 is rotationally driven to stir the magnetic particle solution.

  After the stirring is completed, the horizontal / vertical moving mechanism 900 is operated to raise the stirring body 202 vertically to remove the stirring body 202 from the magnetic particle container. Next, the stirrer 202 is moved horizontally to the upper part of the stirrer cleaning tank, and then the stirrer 202 is vertically lowered to insert the stirrer rod into the cleaning tank, and the stirrer 202 is driven to rotate to clean the stirrer 202. Do. After washing, the magnetic particle stirring arm 108 (arm part) of the horizontal / vertical moving mechanism 900 is returned to the initial position by the same operation.

  Next, the magnetic particle container and the stirring member will be described with reference to FIG.

  The magnetic particle container 300 has an opening 350 on the top. The opening 350 has a diameter of 11 mm and an inner diameter of 18 mm. Although it is desirable to reduce the diameter of the opening of the solution container in order to suppress the evaporation concentration of the magnetic particle solution and to reduce the risk of contamination by contaminants from the outside environment, it is disadvantageous in terms of reducing the long diameter (reducing the size) of the stirring member.

  On the other hand, the stirring body 202 has a stirring rotating shaft 250 and a large stirring blade 302 and a small stirring blade 303 provided at the lower end of the stirring rotating shaft 250. The stirring blades 302 and 303 are provided at symmetrical positions, but are asymmetric in size. Three or four stirring blades may be used. It is desirable to arrange the sizes from a large size to a small size. The stirring blade is placed close to the inner bottom of the magnetic particle container 300 to about 3 mm.

  In this embodiment, the stirring blade 302 having a large radial extension has a blade length of 5.5 mm, and the stirring blade having a small extension has a blade length of 1.5. The total length of the stirring blades straddling the stirring blades 302 and 303 is 7 mm. Since the opening 350 has a diameter of 11 mm, when the stirrer 202 is inserted into the magnetic particle container 300, a gap of 2 mm on one side (4 mm in total) is secured. Since there is such a gap, the stirring member 202 can be inserted without touching the lip of the opening 350.

  Now, the magnetic particle solution is stirred by the rotation of the stirring body 202. The following stirring is performed in relation to the shape of the large stirring blade 302 and the small stirring blade 303 and the weight of the magnetic particle solution.

  (1) By rotating the stirring body while swirling, an up-and-down circulating flow in which the lower layer and upper layer solutions are efficiently mixed is generated, and the settled magnetic particles flow and efficiently disperse without any spots. That is, since the magnetic particle solution is sent from the center to the outer peripheral direction by the rotation of the stirring blade, a downward flow that descends at the center of the magnetic particle container 300 and an upward flow that rises at the outer peripheral side are generated to form a vertical circulation flow. Further, by placing the stirring blade on the inner bottom side of the magnetic particle solution container, the vertical circulating flow becomes a circulating flow over the entire vertical direction, and the dispersion of the magnetic particles extends over the entire vertical direction. Further, the magnetic particle solution is stirred over a wide range from the center to the outer periphery of the magnetic particle solution container by the stirring blade 302 having a large radial extension and the stirring blade having a small radial extension, and the dispersion of the magnetic particles is efficient. Well done.

  (2) Effectively canceling the aggregation of magnetic particles by giving the maximum velocity vector change by the action of the bottom and side surfaces of the magnetic particle solution container close to the magnetic particles given the maximum velocity by swirling the stirring blade. .

  (3) It is designed to eliminate the shake of the stirring rod during rotation in the magnetic particle solution.

  Further, the function of inserting the stirring body into the magnetic particle solution container and rotating and stirring the stirring body having the large and small stirring blades 302 and 303 will be described with reference to FIGS.

  As described in the problem to be solved by the invention, the long diameter of the stirring member is reduced along with the reduction of the opening diameter of the solution container for the purpose of suppressing the evaporation concentration of the solution containing the magnetic particles and reducing the contamination risk from the outside environment (size). There is a problem that it is difficult to maintain sufficient stirring performance capable of eliminating sedimentation and aggregation of the magnetic particles under the constraints of, for example, reducing the diameter of the particles.

  In order to respond to this problem, the stirring body 202 having the large and small stirring blades 302 and 303 is rotated and stirred.

  As shown in FIG. 4, the axis of the stirring rotating shaft 250 of the stirring body 202 having the large and small stirring blades 302 and 303 is eccentric by 2.0 mm with respect to the center of the opening inner wall 401 provided in the magnetic particle solution container. Keep the position until it is close to the bottom of the magnetic particle solution container. When the shaft center of the stirring rotating shaft 250 is set to the center of the opening inner wall 401 without the eccentricity of 2.0 mm, the outer peripheral end of the large stirring blade 302 comes into contact with the edge of the opening inner wall 401. The stirring body 202 cannot be inserted into the solution container.

  When the diameter of the inner wall 401 of the opening is enlarged, the stirrer 202 can be inserted. However, the problem of increasing the risk of contamination of the contaminant from the outside environment and the suppression of evaporation and concentration of the magnetic particle-containing solution accompanying the increase in the diameter becomes significant. .

  Therefore, the magnetic particle solution container maintains the position where the axis of the stirring rotation shaft 250 of the stirring body 202 having the large and small stirring blades 302 and 303 is eccentric with respect to the center of the inner wall 401 of the opening provided in the magnetic particle solution container. The insertion into the inside is suitable for avoiding the contact with the inner wall 401 of the opening of the stirring blade, the suppression of evaporation and concentration of the solution, or the risk of contamination.

  Subsequently, as shown in FIG. 5, the axis of the stirring rotating shaft 250 is arranged on the opening inner wall 401 so that the outer peripheral tip of the large stirring blade 302 can be close to the inner wall surface 400 of the magnetic particle solution container. Move to a position that is eccentric by 3.0 mm with respect to the center. A small dotted circle 501 in FIG. 5 is a turning trajectory drawn by the revolution of the axis of the revolution axis of the revolution drive unit 802 included in the stirrer drive unit 800 described above. A large dotted circle 500 is a rotation locus drawn by the outer peripheral end of the large stirring blade 302 by the rotation of the stirring body 202.

  The large and small agitating blades 302 and 303 rotate around the axis of the agitation rotating shaft 250 while turning on the turning trajectory of the small dotted circle 501. That is, the large and small stirring blades 302 and 303 rotate while rotating. By this swirling and rotation, the outer peripheral end of the large stirring blade 302 moves and rotates in the magnetic particle solution container while being close to or away from the inner wall surface 400 of the magnetic particle solution container to stir the magnetic particle solution. Further, the small stirring blade 302 rotates and rotates in the center of the magnetic particle solution container to stir the magnetic particle solution. The large and small agitating blades 302 and 303 are rotated efficiently while rotating, so that the magnetic particle solution in the magnetic particle solution container is efficiently agitated throughout the entire surface.

  As described above, the magnetic particle solution is stirred by rotating and rotating the stirring blade having a stirring blade having a large radial extension and a stirring blade having a small extension in the same direction. Even if the size of the magnetic particle solution is not increased, the stirrer is inserted, and the magnetic particle solution in the magnetic particle solution container can be efficiently stirred without any unevenness.

  The horizontal / vertical moving mechanism unit 900 operates to insert and withdraw the stirrer from the magnetic particle solution container. Further, the horizontal / vertical moving mechanism 900 is close enough that the outer peripheral end of the large stirring blade does not contact the inner peripheral surface of the magnetic particle solution container when the stirring member inserted in the magnetic particle solution container rotates while rotating. It is adjusted by carrying the stirring body in the horizontal direction.

  In the adjustment to carry the stirring body in the horizontal direction, the movement of moving the magnetic particle solution container horizontally by rotating the reagent disk on which the magnetic particle solution container is placed is combined with the inner peripheral surface of the magnetic particle solution container. The appropriate position where the outer peripheral edge of the large stirring blade is close can be adjusted.

  Next, the dimensional ratio of the stirrer and the magnetic particle solution container will be described.

  Let a be the diameter of the inner wall of the opening of the magnetic particle solution container. The stirrer has a stirring rotation shaft and two large (long) and small (short) stirring blades extending radially from the stirring rotation shaft. The length between two stirring blades is b. Let c be the radial length of the large (long) stirring blade. Let d be the radial length of a small (short) stirring blade. The length of the large (long) and small (short) stirring blades is c ≧ d. However, the radial length c of the large (long) stirring blade is equal to or less than the radius e in the magnetic particle solution container (e ≧ c).

  After the stirrer with such large (long) and small (short) stirrer blades is inserted close to the bottom of the magnetic particle solution container, large (long) stirring is performed when the stirrer rotates and rotates. The blade moves the stirrer to a position where the outer peripheral tip can be close to the inner wall of the magnetic particle solution container to start rotation. As a result, the large (long) stirring blade rotates at a maximum turning radius c in a state of being close to the inner bottom surface and the inner side surface of the magnetic particle solution container, thereby stirring the magnetic particle solution.

It is a figure which concerns on the Example of this invention, and shows the whole structure of a chemical analyzer provided with the stirring apparatus of a magnetic particle solution. It is a figure which concerns on the Example of this invention and shows the stirring apparatus of a magnetic particle solution. It is a longitudinal section which concerns on the Example of this invention and shows a stirring body and a magnetic particle solution container. FIG. 6 is a top view according to an embodiment of the present invention, as viewed from above in a state where a stirring body is inserted into a magnetic particle solution container. According to the embodiment of the present invention, when the stirrer is inserted into the magnetic particle solution container and the stirrer rotates while rotating, the outer peripheral tip of the large stirring blade can be close to the inner wall surface of the magnetic particle solution container. It is the top view which looked at the state which moved the stirring body to the top from the top. FIG. 6 is a schematic diagram simply showing an agitator driving unit including a revolution driving unit and a rotation rotating unit according to an embodiment of the present invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 100 ... Chemical analyzer 101 ... Sample dispensing tip / reaction container disposal hole 102 ... Sample dispensing chip buffer 103 ... Sample 104 ... Reaction liquid stirring mechanism 105 ... Sample dispensing tip / reaction container transport mechanism 106 ... Sample dispensing tip / Reaction vessel 107 ... Incubator disk 108 ... Magnetic particle stirring arm 109 ... Washing tank 110 ... Reagent dispensing pipettor 111 ... Reagent container 112 ... Reagent disk 113 ... Reaction liquid suction nozzle 114 ... Detector 115 ... Sample transport line 116 ... Sample dispensing Pipetter 200 ... stirring device 202 ... stirring body 250 ... stirring rotary shaft 300 ... magnetic particle solution container 350 ... magnetic particle solution container opening 302 ... large stirring blade 303 ... small stirring blade 400 ... inner wall surface 401 of magnetic particle solution container ... Opening inner wall 500 of magnetic particle solution container ... large dotted line 501 ... small dotted circle 800 ... stirring member driving unit 801 ... chuck 802 ... revolution driving unit 803 ... rotation rotation unit 804 ... supporting arm 900 ... horizontal vertical movement mechanism portion 901 ... Axis 902 ... vertical movement rotation driver

Claims (13)

In a magnetic particle solution stirring device comprising a stirring body for stirring a solution containing magnetic particles in a magnetic particle solution container,
The stirrer has a stirring rotating shaft, a stirring blade having a large radial extension provided on the stirring rotating shaft, and a stirring blade having a small extension in the same direction. In a magnetic particle solution stirring device comprising a stirring body for stirring a solution containing magnetic particles in a magnetic particle solution container,
The stirring body has a stirring rotating shaft and a stirring blade provided on the stirring rotating shaft,
The stirring apparatus for a magnetic particle solution, wherein the stirring rotation shaft rotates and rotates while rotating. In a magnetic particle solution stirring device comprising a stirring body for stirring a solution containing magnetic particles in a magnetic particle solution container,
The stirrer has a stirring rotation shaft, a stirring blade having a large radial extension provided on the stirring rotation shaft, and a stirring blade having a small extension in the same direction,
The stirring apparatus for a magnetic particle solution, wherein the stirring rotation shaft rotates and rotates while rotating. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 1-3,
The magnetic particle solution stirring apparatus, wherein the stirring blade is provided on a lower end side of the stirring rotating shaft. Magnetic particles comprising a stirrer that stirs a solution containing magnetic particles in a magnetic particle solution container, a stirrer rotation drive unit that rotationally drives the stirrer, and a horizontal and vertical movement mechanism unit that moves the stirrer horizontally and vertically In the solution stirring device,
The stirring body has a stirring rotating shaft and a stirring blade provided on the stirring rotating shaft,
The stirrer rotation drive unit includes a stirrer drive mechanism that rotates and rotates while rotating the stirring rotation shaft. In the stirring apparatus of the magnetic particle solution according to claim 5,
The horizontal / vertical moving mechanism is provided in a vertically extending and rotating pivot that is vertically movable, a vertically moving and pivoting drive that vertically moves and pivots the pivot, and an upper end side of the pivot. A magnetic particle solution agitator having an arm portion for supporting the agitator rotation driving portion on an end side separated in a radial direction. In the stirring apparatus of the magnetic particle solution according to claim 5,
The stirrer drive mechanism has a revolution shaft, a rotation shaft, and a support arm that is provided on the revolution shaft and supports the rotation shaft, and the shaft center of the rotation shaft and the stirrer is concentric. A magnetic particle solution stirring apparatus. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 5-7,
The stirrer has a stirring rotating shaft, a stirring blade having a large radial extension provided on the stirring rotating shaft, and a stirring blade having a small extension in the same direction. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 5-7,
The magnetic particle solution stirring apparatus, wherein the stirring blade of the stirring body is provided at a lower end of the stirring rotating shaft. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 5-9,
In the operation in which the agitation rotating shaft rotates while rotating, the agitating blade moves along the inner surface of the solution container while repeating the approach and separation to stir the solution. Stirrer for magnetic particle solution. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 5-9,
The horizontal / vertical moving mechanism is moved in the horizontal direction so that the stirring blades of the stirring body are brought close to contact with the inner surface of the solution container by the rotation of the stirring rotating shaft while rotating. An apparatus for stirring a magnetic particle solution, characterized by carrying a stirring body. In the stirring apparatus of the magnetic particle solution according to claim 9,
Stirring the magnetic particle solution, carrying the stirrer by moving the horizontal / vertical moving mechanism portion downward so that the stirrer blade of the stirrer approaches the inner bottom surface of the solution container as much as desired. apparatus. In the stirring apparatus of the magnetic particle solution as described in any one of Claims 5-11 and 12,
A solution container disk that can be moved horizontally by placing the solution container,
The horizontal and vertical movements so that the stirrer blades of the stirrer are rotated and rotated while the stirrer rotating shaft is rotated so that the stirrer blades of the stirrer approach the inner surface of the solution container as much as possible. An apparatus for stirring a magnetic particle solution, wherein the horizontal movement of a mechanism portion and a solution container disk is adjusted mutually.

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