CN115252677A - Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal prebiotics and preparation method thereof - Google Patents
Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal prebiotics and preparation method thereof Download PDFInfo
- Publication number
- CN115252677A CN115252677A CN202210546709.6A CN202210546709A CN115252677A CN 115252677 A CN115252677 A CN 115252677A CN 202210546709 A CN202210546709 A CN 202210546709A CN 115252677 A CN115252677 A CN 115252677A
- Authority
- CN
- China
- Prior art keywords
- mulberry leaf
- preparation
- powder
- prebiotics
- intestinal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000000249 Morus alba Species 0.000 title claims abstract description 99
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 99
- 235000013406 prebiotics Nutrition 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 239000008280 blood Substances 0.000 title claims abstract description 35
- 210000004369 blood Anatomy 0.000 title claims abstract description 35
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 28
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 17
- 230000001276 controlling effect Effects 0.000 title claims abstract description 10
- 230000014101 glucose homeostasis Effects 0.000 title claims description 18
- 239000000843 powder Substances 0.000 claims abstract description 53
- 150000004676 glycans Chemical class 0.000 claims abstract description 32
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 32
- 239000005017 polysaccharide Substances 0.000 claims abstract description 32
- 229930013930 alkaloid Natural products 0.000 claims abstract description 27
- 150000003797 alkaloid derivatives Chemical class 0.000 claims abstract description 26
- 108010013296 Sericins Proteins 0.000 claims abstract description 22
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000011248 coating agent Substances 0.000 claims abstract description 14
- 238000000576 coating method Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000006041 probiotic Substances 0.000 claims abstract description 9
- 235000018291 probiotics Nutrition 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims abstract description 8
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims abstract description 8
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims abstract description 8
- 230000000529 probiotic effect Effects 0.000 claims abstract description 8
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000000227 grinding Methods 0.000 claims abstract description 6
- 238000007873 sieving Methods 0.000 claims abstract description 6
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 239000000725 suspension Substances 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- 239000006228 supernatant Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 241000255789 Bombyx mori Species 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 8
- 239000003729 cation exchange resin Substances 0.000 claims description 8
- 238000000502 dialysis Methods 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 238000002386 leaching Methods 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 230000013632 homeostatic process Effects 0.000 claims description 2
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims 2
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 238000005469 granulation Methods 0.000 abstract 1
- 230000003179 granulation Effects 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 13
- 239000000706 filtrate Substances 0.000 description 12
- 239000002253 acid Substances 0.000 description 6
- 238000001694 spray drying Methods 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- 239000003480 eluent Substances 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 238000002390 rotary evaporation Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000010171 animal model Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000013268 sustained release Methods 0.000 description 2
- 239000012730 sustained-release form Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000009693 chronic damage Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000021196 dietary intervention Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000004190 glucose uptake Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 235000021069 high fat-high sugar diet Nutrition 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 229960003943 hypromellose Drugs 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000893 inhibin Substances 0.000 description 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000000291 postprandial effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Diabetes (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Alternative & Traditional Medicine (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a mulberry leaf prebiotic for regulating and controlling blood sugar steady state based on intestinal probiotic and a preparation method thereof, which comprises a) granulation: mixing mulberry leaf alkaloid powder and mulberry leaf polysaccharide powder in a ratio of 1: 0.5-4, adding a solvent, stirring into a dough or paste, drying, grinding and sieving to obtain mulberry leaf alkaloid and polysaccharide mixed particles, b) preparing a liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the weight ratio of 1: 0.1-1, and obtaining the slow-release gel coating solution and c) coating: mixing the mulberry leaf alkaloid and polysaccharide mixed particles prepared in the step a) in a ratio of 1: 10-20 parts by weight of the solution is added into the slow-release gel coating solution prepared in the step b), and the solution is stirred into suspension and sprayed to dry, so that a finished product is obtained. The application can realize the continuous control effect on the blood sugar, regulate and control intestinal flora and maintain the blood sugar stability of the diabetic.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of blood sugar regulation, in particular to a mulberry leaf prebiotics for regulating blood sugar steady state based on intestinal probiotic and a preparation method thereof.
[ background of the invention ]
Blood glucose disorders are metabolic diseases characterized by hyperglycemia, which may lead to chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels or nerves, thereby causing a series of complications. Research has confirmed that there is a significant correlation between gut microbes and the development of diabetes, and thus regulation of gut microbes becomes one of the important means of controlling blood glucose (Chen K, chen H, faas MM, haan B, li JH, et al. Specific insulin-type free fibers technologies monitoring approach to automatic immunity diabetes by modulating gut immunity, barrier function, and microbial homeostatis. Molecular Nutrition & Food Research,2017,61 (8), 1601006.). Dietary fiber and Prebiotics, among other reasonable dietary interventions, can ameliorate sugar metabolism disorders by modulating The gut flora, with safety, economy and convenience features (Glenn RG, robert H, mary ES, susan LP, raylene AR, et al. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus on The definition and scope of microbiocs. Nature Reviews Gastroenterology and Heatology, 2017,14 491-502). Mulberry leaves are medicinal and edible traditional Chinese medicinal materials, and are used for treating diabetes (diabetes) in ancient times, and the compendium of materia medica clearly records that mulberry leaves are decocted to replace tea and can stop diabetes. Modern pharmacological studies have found that alkaloids in mulberry leaves can regulate intestinal microorganisms and maintain blood glucose homeostasis (Li Y, ji D, zhong S, lin T, lv Z, hu G, wang x.1-deoxygeniomyces inhibin glucose uptake and acellularies glucose metabolism in microbial metabolism, 2013.
[ summary of the invention ]
The invention aims to solve the problems in the prior art and provides a mulberry leaf prebiotic for regulating and controlling blood sugar steady state based on intestinal probiotic and a preparation method thereof.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a preparation method of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal prebiotics comprises the following steps:
a) Granulating: mixing mulberry leaf alkaloid powder and mulberry leaf polysaccharide powder in a ratio of 1: 0.5-4, adding a solvent, stirring into a dough or paste, and drying, grinding and sieving to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the weight ratio of 1: uniformly mixing the components in a volume ratio of 0.1-1 to obtain a slow-release gel coating solution;
c) Coating: mixing the mulberry leaf alkaloid and polysaccharide mixed particles prepared in the step a) in a ratio of 1: 10-20 parts by weight of the mixture is added into the slow-release gel coating solution prepared in the step b), and the mixture is stirred into suspension and is sprayed to dry, so that a finished product is obtained.
Preferably, in the step a), the mulberry leaf alkaloid powder is prepared by leaching mulberry leaf powder with an organic solvent, volatilizing to remove the organic solvent in the leaching supernatant, adsorbing and eluting with a cation exchange resin, and drying.
Furthermore, in the step a), the extracting the mulberry leaf powder by using the organic solvent is to extract the mulberry leaf powder by mixing the mulberry leaf powder with a solvent of 1: 30-50 percent of ethanol is added into the ethanol with the mass volume ratio of 50-70 percent, and the extraction is carried out for 4-6 h at the temperature of 40-60 ℃ and the ultrasonic power of 1000-1300W.
Further, in the step a), the mulberry leaf powder is prepared by picking and cutting fresh mulberry leaves, standing at room temperature for 1-4 h, then naturally drying in the sun or drying by an oven at 50-60 ℃, crushing again by a crusher and sieving at 30-80 meshes to obtain the mulberry leaf powder.
Further, in the step a), after leaching is completed, filtering to remove filter residues and collecting filtrate, centrifuging the filtrate at 8000-10000 rpm and collecting supernatant, steaming the supernatant to remove alcohol, adsorbing for 12-24 hours by using an acid type cation exchange resin, firstly washing the acid type cation exchange resin by using tap water to remove impurities, then eluting the acid type cation exchange resin by using 0.25-0.5 mol/L ammonia water and collecting eluent, steaming the eluent to remove the ammonia water, concentrating to a small volume, centrifuging to obtain supernatant, adsorbing, eluting and concentrating the supernatant again, and then freezing or spray drying to obtain the product.
Preferably, in the step a), the mulberry polysaccharide powder is prepared by extracting mulberry leaf powder with water, filtering and concentrating the extract, precipitating the concentrate with ethanol, separating with a dialysis membrane, and drying.
Further, in the step a), the step of extracting the mulberry leaf powder with water specifically comprises the step of adding the mulberry leaf powder or the mulberry leaf powder residue after alkaloid extraction (according to the weight of the original mulberry leaf powder) into the mixture with a feed-liquid ratio of 1: boiling 30-50% water for 4-6 h. Wherein. The preparation method of folium Mori powder is the same as above.
Further, in the step a), after water extraction is completed, filter residue is removed and filtrate is collected, the filtrate is centrifuged at 8000-10000 rpm and supernatant is collected, the supernatant is concentrated to 1/5-1/10 of the original volume, ethanol with 2-4 times of the volume of the concentrated solution is slowly added, after overnight precipitation, the supernatant is centrifuged at 8000-1000 r/min and poured out, the precipitate is fully dissolved by hot water to obtain crude polysaccharide solution, the crude polysaccharide solution is separated by using dialysis membranes of 10kDa and 100kDa, and after rotary evaporation and concentration to a smaller volume, the polysaccharide is obtained by freeze drying or spray drying.
Preferably, in the step a), the mulberry polysaccharides in the mulberry polysaccharide powder have a molecular weight of 10-100 kDa.
Preferably, in the step a), 20-50% ethanol solution is added into the uniformly mixed mulberry leaf alkaloid powder and mulberry leaf polysaccharide powder, the mixture is stirred into a dough or paste, the mixture is dried at the temperature of below 60 ℃, then the mixture is ground by a grinding instrument, and the ground mixture is sieved by a 30-50 mesh sieve.
Preferably, in the step b), the sericin concentrated solution is obtained by dissolving sericin powder in water, wherein the sericin powder is prepared by dissolving silkworm cocoon shells with an alkali solution, filtering and concentrating the solution, separating with a dialysis membrane, and drying.
Further, in the step b), dissolving the silkworm cocoon shells by using the alkali liquor specifically comprises adding a material-liquid ratio of 1: 40-100 parts of sodium carbonate solution with the mass fraction of 1-5% and heating and boiling for 1-3 hours, and continuously stirring. Wherein the silkworm cocoon shell is an oak silkworm cocoon shell or a domestic silkworm cocoon shell.
Further, in the step b), after the dissolution is finished, filtering to remove filter residues and collecting filtrate, concentrating the filtrate to 1/5-1/10 of the original volume, separating the concentrated solution by using a 10kDa dialysis membrane, and performing freeze drying or spray drying to obtain the product.
Preferably, in the step b), the molecular weight of the sericin in the sericin concentrate is more than 10kDa.
Preferably, in the step c), the sustained-release gel coating solution is cooled to 50 to 65 ℃.
Preferably, in the step c), the stirring speed is 800 to 1200r/min.
A mulberry leaf prebiotic for regulating blood sugar homeostasis based on intestinal prebiotics is prepared by the preparation method.
The invention has the beneficial effects that:
the invention uses mulberry leaves and natural silk which are plant parts used as both medicine and food as main raw materials, firstly carries out gradient separation to extract mulberry leaf alkaloid and mulberry leaf polysaccharide which has the functions of resisting oxidation, reducing blood sugar and the like and can play a synergistic synergy effect with the mulberry leaf alkaloid to maintain the blood sugar steady-state effect, then carries out separation and extraction to obtain adhesive macromolecular sericin which has physical characteristics of water absorption, gelation, modification and the like and can be used as a good functional slow-release matrix in silkworm cocoons, then optimizes the synergistic ratio of the mulberry leaf alkaloid and the mulberry leaf polysaccharide in the mulberry leaves and prepares mulberry leaf alkaloid and polysaccharide mixed particles, and finally embeds the mulberry leaf alkaloid and polysaccharide mixed particles in a slow-release gel outer membrane formed by physical crosslinking of sericin and hydroxypropyl methylcellulose to finally prepare the mulberry leaf prebiotic based on intestinal probiotic regulation and control of the blood sugar steady-state.
The features and advantages of the present invention will be described in detail by embodiments in conjunction with the accompanying drawings.
[ description of the drawings ]
FIG. 1 is a graph comparing the effect of mulberry leaf prebiotics produced by the present invention on glucose tolerance in mice based on intestinal prebiotics regulation of blood glucose homeostasis;
FIG. 2 is the effect of mulberry leaf prebiotics produced by the present invention on the intestinal microbial diversity of mice based on intestinal prebiotics to regulate blood glucose homeostasis.
In FIG. 2, a and b are the effect on α diversity, the effect of the reaction on species abundance; c is the effect on beta diversity and the effect of the reaction on the flora structure.
[ detailed description ] A
Example one, preparation of mulberry leaf prebiotics to regulate blood glucose homeostasis based on intestinal prebiotics:
a preparation method of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal prebiotics comprises the following steps:
a) And (3) granulating:
a1 Preparation of mulberry leaf powder: picking fresh mulberry leaves at 5-10 months per year, cutting, standing at room temperature for 1-4 h to make the milk of the mulberry leaves fully overflow, then naturally drying in the sun or drying at 60 ℃ by using an oven, crushing again by using a crusher and sieving at 30-80 meshes to obtain mulberry leaf powder;
a2 Preparation of mulberry leaf alkaloid powder: mixing mulberry leaf powder in a proportion of 1: adding 30-50% by mass volume of the mixture into 50-70% ethanol, extracting for 4-6 h at 40-60 ℃ and under the ultrasonic power of 1000-1300W, after leaching is completed, filtering to remove filter residues and collecting filtrate, centrifuging the filtrate at 8000-10000 rpm, collecting supernatant, carrying out rotary evaporation on the supernatant to remove the ethanol, adsorbing for 12-24 h by using acid type cation exchange resin, washing the acid type cation exchange resin by tap water to remove impurities, eluting the acid type cation exchange resin by using 0.25-0.5 mol/L ammonia water, collecting eluent, carrying out rotary evaporation on the eluent to remove the ammonia water, concentrating to a small volume, centrifuging to obtain supernatant, carrying out upper adsorption, elution and concentration on the supernatant again, and then freezing or spray drying to obtain mulberry leaf alkaloid powder;
a3 Preparation of mulberry leaf polysaccharide powder: adding the mulberry leaf powder prepared in the step a 1) or the mulberry leaf powder residue after alkaloid extraction (according to the weight of the original mulberry leaf powder) into a mixture with a feed-liquid ratio of 1: boiling 30-50 parts of water for extraction for 4-6 hours, filtering to remove filter residues and collect filtrate after water extraction is finished, centrifuging the filtrate at 8000-10000 rpm and collecting supernatant, concentrating the supernatant to 1/5-1/10 of the original volume, slowly adding ethanol with the volume being 3 times that of the concentrated solution, centrifuging at 8000-1000 r/min after precipitation over night and pouring out the supernatant, fully dissolving the precipitate by using hot water to obtain crude polysaccharide solution, separating out mulberry leaf polysaccharide with the molecular weight of 10-100 kDa in the crude polysaccharide solution by using 10-kDa and 100-kDa dialysis membranes, concentrating to a smaller volume by rotary volatilization, and freeze-drying or spray-drying to obtain the mulberry leaf polysaccharide powder;
a4 Preparation of mixed microparticles of mulberry leaf alkaloid and polysaccharide: mixing the mulberry leaf alkaloid powder prepared in the step a 2) and the mulberry leaf polysaccharide powder prepared in the step a 3) according to the weight ratio of 1:0.5 to 4, adding 20 to 50 percent ethanol solution, stirring into a dough or paste, drying at the temperature of below 60 ℃, grinding by using a grinding instrument, and sieving by using a 40-mesh sieve to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid:
b1 Preparation of sericin powder: adding a material-liquid ratio of 1: 40-100 parts of sodium carbonate solution with the mass fraction of 1-5% is heated and boiled for 2 hours, the stirring is continuously carried out during the heating, the filter residue is removed by filtration, the filtrate is collected, the filtrate is concentrated to 1/5-1/10 of the original volume, sericin with the molecular weight of more than 10kDa in the concentrated solution is separated by a 10kDa dialysis membrane, and then the sericin powder is obtained by freeze drying or spray drying;
b2 ): preparation of sericin concentrate: dissolving the sericin powder prepared in the step b 1) in hot water to prepare a sericin concentrated solution with the concentration of 50-100 g/L;
b3 Preparation of hypromellose E50 solution: dissolving hydroxypropyl methylcellulose E50 in hot water to prepare a hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5%;
b4 Preparation of sustained-release gel coating solution: mixing 50-100 g/L of sericin concentrated solution prepared in the step b 2) and 2-5% of hydroxypropyl methylcellulose E50 solution prepared in the step b 3) in a mass ratio of 1: uniformly mixing the components in a volume ratio of 0.1-1, and quickly stirring the components in a stirrer at a speed of 1000r/min to obtain a slow-release gel coating solution;
c) Coating: mixing the mulberry leaf alkaloid and polysaccharide mixed particles prepared in the step a 4) in a ratio of 1: 10-20 weight volume percent of the mixture is added into the slow-release gel coating liquid which is prepared in the step b 4) and cooled to 50-65 ℃, and the mixture is stirred into suspension at 800-1200 r/min and is sprayed and dried to obtain a finished product.
Example two, in vivo evaluation test of mulberry leaf prebiotics based on intestinal prebiotics regulation of blood glucose homeostasis:
experimental animals and feeding conditions: 30 clean female ICR mice (Hangzhou photon source laboratory animal science and technology limited company [ production permit: SCXK (Zhe) 2019-0004 ]) with the weight of 18-20 g. Barrier system laboratory, temperature: 25 +/-1 ℃; humidity: 50 to 70 percent; illumination: 150-200Lx, 12h alternate in light and shade; noise <50dB. Drinking water: tap water. A breeding mode: free diet, mice were fed with sufficient feed and water in cages, and 10 mice were fed per cage.
Test design and measurement indexes: the mice were randomly divided into 3 groups of 10 mice each, and the control group was given a normal diet, the diabetes model group and the prebiotic intervention group were given a high-fat high-sugar diet for 8 weeks and finally injected intraperitoneally with Streptozotocin (STZ) at a dose of 40mg/kg body weight for 5 days continuously. The prebiotics intervention group is perfused with 200mg/kg body weight of mulberry leaf prebiotics prepared by the process every day, and the control group and the model group are perfused with ultrapure water with the same volume as the stomach. After 8 weeks, the mice are fasted and are not forbidden to be watered for 12 hours, and then blood is taken from tail veins, and the fasting blood glucose value of the mice is measured by using a OneTouchR Ultra glucometer. Ultra-pure water is infused into the stomach of different groups or 0.1ml/10g of sucrose solution (prepared by normal saline) of 0.3g/ml is instantly orally administered into each group after 30min of the mulberry leaf prebiotics prepared by the process, and the blood sugar of 30min, 60min and 120min after sugar administration is measured. The 16SrRNA gene sequencing analysis is carried out on the microorganism by taking the cecal content of the mouse.
And (3) data analysis: statistical analysis was performed using SPSS16 software, all data are averaged. + -. Standard deviation
The test results are shown to be evaluated using the t-test.
The result shows that the mulberry leaf prebiotics prepared by the process obviously improves the sugar tolerance of diabetic mice (figure 1), controls the postprandial blood sugar steady state, improves the diversity of intestinal microorganisms of the diabetic mice (figure 2) and promotes the normal flora structure of the diabetic mice (figure 2). The results show that the mulberry leaf prebiotics produced by the process can regulate the intestinal flora of diabetic mice and control the steady state of blood sugar.
The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.
Claims (10)
1. A preparation method of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal prebiotics is characterized by comprising the following steps:
a) And (3) granulating: mixing mulberry leaf alkaloid powder and mulberry leaf polysaccharide powder in a ratio of 1: 0.5-4, adding a solvent, stirring into a dough or paste, and drying, grinding and sieving to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the weight ratio of 1: uniformly mixing the components in a volume ratio of 0.1-1 to obtain a slow-release gel coating solution;
c) Coating: mixing the mulberry leaf alkaloid and polysaccharide mixed particles prepared in the step a) in a ratio of 1: 10-20 parts by weight of the mixture is added into the slow-release gel coating solution prepared in the step b), and the mixture is stirred into suspension and is sprayed to dry, so that a finished product is obtained.
2. The preparation method of the mulberry leaf prebiotic for regulating and controlling blood glucose homeostasis based on intestinal prebiotics according to claim 1, wherein the preparation method comprises the following steps: in the step a), the mulberry leaf alkaloid powder is prepared by leaching mulberry leaf powder by using an organic solvent, volatilizing to remove the organic solvent in the leaching supernatant, adsorbing and eluting by using cation exchange resin, and drying.
3. The preparation method of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal probiotic bacteria according to claim 2, wherein the preparation method comprises the following steps: in the step a), the extracting of the mulberry leaf powder by using the organic solvent is to extract the mulberry leaf powder by mixing the mulberry leaf powder with a solvent of 1: 30-50 percent of ethanol is added into the ethanol with the mass volume ratio of 50-70 percent, and the extraction is carried out for 4-6 h at the temperature of 40-60 ℃ and the ultrasonic power of 1000-1300W.
4. The preparation method of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal probiotic bacteria according to claim 1, wherein the preparation method comprises the following steps: in the step a), the mulberry leaf polysaccharide powder is prepared by firstly extracting mulberry leaf powder with water, filtering and concentrating the extract, precipitating the concentrated solution with alcohol, separating by using a dialysis membrane, and drying.
5. The preparation method of mulberry leaf prebiotics based on intestinal probiotic controlled glycemia homeostasis as claimed in claim 4, wherein the preparation method comprises the following steps: in the step a), the step of extracting the mulberry leaf powder by water is to add the mulberry leaf powder or the mulberry leaf powder residue after the alkaloid extraction into the mixture in a ratio of 1: boiling 30-50% water for 4-6 h.
6. The preparation method of the mulberry leaf prebiotic for regulating and controlling blood glucose homeostasis based on intestinal prebiotics according to claim 1, wherein the preparation method comprises the following steps: in the step a), the molecular weight of the mulberry leaf polysaccharide in the mulberry leaf polysaccharide powder is 10-100 kDa.
7. The preparation method of the mulberry leaf prebiotic for regulating and controlling blood glucose homeostasis based on intestinal prebiotics according to claim 1, wherein the preparation method comprises the following steps: in the step b), the sericin concentrated solution is obtained by dissolving sericin powder in water, wherein the sericin powder is prepared by dissolving a silkworm cocoon shell with an alkali solution, filtering and concentrating the dissolved solution, separating with a dialysis membrane, and drying.
8. The preparation method of the mulberry leaf prebiotic for regulating and controlling blood glucose homeostasis based on intestinal prebiotics according to claim 7, wherein the preparation method comprises the following steps: in the step b), dissolving the silkworm cocoon shells by using alkali liquor specifically comprises the following steps of adding the raw materials into the silkworm cocoon shells according to a ratio of 1: 40-100 parts of sodium carbonate solution with the mass fraction of 1-5%, and heating and boiling for 1-3 hours while continuously stirring.
9. The preparation method of the mulberry leaf prebiotic for regulating and controlling blood glucose homeostasis based on intestinal prebiotics according to claim 1, wherein the preparation method comprises the following steps: in the step b), the molecular weight of the sericin in the sericin concentrate is greater than 10kDa.
10. A mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotic prepared by the preparation method of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210546709.6A CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210546709.6A CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115252677A true CN115252677A (en) | 2022-11-01 |
| CN115252677B CN115252677B (en) | 2023-05-12 |
Family
ID=83760577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210546709.6A Active CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115252677B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006257000A (en) * | 2005-03-16 | 2006-09-28 | Iwase Cosfa Kk | Sericin derivative and composition containing the same |
| CN1850166A (en) * | 2006-03-08 | 2006-10-25 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
| CN105535112A (en) * | 2015-12-29 | 2016-05-04 | 浙江省农业科学院 | Extraction technology of hypoglycemic medicinal active substances of mulberry leaves and mulberries and formula |
| WO2019013172A1 (en) * | 2017-07-12 | 2019-01-17 | 株式会社 きものブレイン | Health food containing silkworm cocoon-derived ingredient |
-
2022
- 2022-05-18 CN CN202210546709.6A patent/CN115252677B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006257000A (en) * | 2005-03-16 | 2006-09-28 | Iwase Cosfa Kk | Sericin derivative and composition containing the same |
| CN1850166A (en) * | 2006-03-08 | 2006-10-25 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
| CN105535112A (en) * | 2015-12-29 | 2016-05-04 | 浙江省农业科学院 | Extraction technology of hypoglycemic medicinal active substances of mulberry leaves and mulberries and formula |
| WO2019013172A1 (en) * | 2017-07-12 | 2019-01-17 | 株式会社 きものブレイン | Health food containing silkworm cocoon-derived ingredient |
Non-Patent Citations (2)
| Title |
|---|
| 玄光善 等: "桑叶有效成分降糖作用研究", 食品科学 * |
| 肖肖 等: "丝胶蛋白的结构、性能及生物医学应用", 化学进展 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115252677B (en) | 2023-05-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107412721B (en) | Blood sugar-reducing bitter gourd polypeptide compound capsule and preparation method thereof | |
| CN103719880A (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN107412720A (en) | A kind of bitter gourd polypeptide compound plant medicine for treating diabetes and preparation method thereof | |
| CN110772630A (en) | Compound bitter gourd peptide oral liquid for activating insulin receptor and regulating blood sugar and preparation method thereof | |
| CN110201067A (en) | A kind of Ampelopsis grossedentata extrat and its preparation method and application | |
| WO2021042700A1 (en) | Method for extracting hemp polysaccharides, product obtained thereby and use thereof | |
| US20220370541A1 (en) | Activated insulin, compound momordica charantia peptide oral medicine for treatment of diabetes, and preparation method | |
| CN107778376B (en) | A kind of preparation method and applications of radix tetrastigme polysaccharide | |
| CN112535239B (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
| CN115252677A (en) | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal prebiotics and preparation method thereof | |
| CN101709076A (en) | Method for preparing hoof nail polypeptide | |
| CN111803531A (en) | Preparation method and application of dandelion leaf water-soluble crude extract | |
| CN102020723B (en) | Method for continuously extracting low-ester pectin and microcrystalline cellulose from jerusalem artichoke stalks | |
| CN109232757B (en) | Walnut leaf polysaccharide extract and application thereof | |
| CN110179128A (en) | Compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide and preparation method thereof | |
| CN106539088B (en) | Preparation method of functional sucrose with blood sugar reducing function | |
| CN112205621A (en) | Functional food suitable for diabetics | |
| CN106994142B (en) | Loquat extract and loquat black tea extract buccal tablets capable of reducing blood sugar prepared from loquat extract | |
| CN110960663A (en) | Preparation method of Xiaochaihu granules | |
| CN111789256B (en) | Pitaya flower polysaccharide composition with insulin balancing effect | |
| CN115006355B (en) | Preparation method of veterinary coptis chinensis granules with full-component extraction | |
| CN114558051B (en) | Chinese medicinal composition for treating diabetes and preparation method thereof | |
| CN115381874B (en) | Traditional Chinese medicine composition and granule for treating chicken noninfectious diarrhea and preparation method thereof | |
| CN116158535B (en) | Mulberry leaf protein-mulberry anthocyanin composite emulsion and preparation method thereof | |
| CN109182429B (en) | Mulberry silkworm pupa anticancer active peptide BPP-3 and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |