CN115252677B - Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof - Google Patents
Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof Download PDFInfo
- Publication number
- CN115252677B CN115252677B CN202210546709.6A CN202210546709A CN115252677B CN 115252677 B CN115252677 B CN 115252677B CN 202210546709 A CN202210546709 A CN 202210546709A CN 115252677 B CN115252677 B CN 115252677B
- Authority
- CN
- China
- Prior art keywords
- mulberry leaf
- powder
- blood glucose
- glucose homeostasis
- prebiotic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 90
- 240000000249 Morus alba Species 0.000 title claims abstract description 90
- 239000008280 blood Substances 0.000 title claims abstract description 35
- 210000004369 blood Anatomy 0.000 title claims abstract description 35
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 31
- 235000013406 prebiotics Nutrition 0.000 title claims abstract description 30
- 239000006041 probiotic Substances 0.000 title claims abstract description 23
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 23
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 230000001276 controlling effect Effects 0.000 title claims abstract description 8
- 230000014101 glucose homeostasis Effects 0.000 title claims description 17
- 239000000843 powder Substances 0.000 claims abstract description 51
- 150000004676 glycans Chemical class 0.000 claims abstract description 33
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 33
- 239000005017 polysaccharide Substances 0.000 claims abstract description 33
- 229930013930 alkaloid Natural products 0.000 claims abstract description 30
- 108010013296 Sericins Proteins 0.000 claims abstract description 22
- 150000003797 alkaloid derivatives Chemical class 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000002156 mixing Methods 0.000 claims abstract description 19
- 239000011248 coating agent Substances 0.000 claims abstract description 14
- 238000000576 coating method Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims abstract description 8
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims abstract description 8
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims abstract description 8
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 4
- 239000000725 suspension Substances 0.000 claims abstract description 4
- 235000011837 pasties Nutrition 0.000 claims abstract description 3
- 239000002904 solvent Substances 0.000 claims abstract description 3
- 238000005507 spraying Methods 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000006228 supernatant Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 9
- 238000000502 dialysis Methods 0.000 claims description 8
- 238000002386 leaching Methods 0.000 claims description 8
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 7
- 239000003729 cation exchange resin Substances 0.000 claims description 7
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 239000000203 mixture Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 24
- 241000699670 Mus sp. Species 0.000 description 14
- 239000000706 filtrate Substances 0.000 description 11
- 206010012601 diabetes mellitus Diseases 0.000 description 10
- 239000008103 glucose Substances 0.000 description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 9
- 244000005700 microbiome Species 0.000 description 6
- 238000001694 spray drying Methods 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 241000255789 Bombyx mori Species 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- LXBIFEVIBLOUGU-JGWLITMVSA-N duvoglustat Chemical compound OC[C@H]1NC[C@H](O)[C@@H](O)[C@@H]1O LXBIFEVIBLOUGU-JGWLITMVSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002641 glycemic effect Effects 0.000 description 2
- 235000009200 high fat diet Nutrition 0.000 description 2
- 230000013632 homeostatic process Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- LXBIFEVIBLOUGU-UHFFFAOYSA-N Deoxymannojirimycin Natural products OCC1NCC(O)C(O)C1O LXBIFEVIBLOUGU-UHFFFAOYSA-N 0.000 description 1
- 229920002670 Fructan Polymers 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000009693 chronic damage Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000021196 dietary intervention Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 235000021069 high fat-high sugar diet Nutrition 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 229960003943 hypromellose Drugs 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000000291 postprandial effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229960001052 streptozocin Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Diabetes (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Alternative & Traditional Medicine (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a mulberry leaf prebiotic for regulating and controlling blood sugar steady state based on intestinal probiotics and a preparation method thereof, comprising the following steps of a) granulating: mixing folium Mori alkaloid powder and folium Mori polysaccharide powder at a ratio of 1: uniformly mixing the components in a weight ratio of 0.5-4, adding a solvent, stirring the mixture into a bulk or pasty state, and then drying, grinding and sieving the mixture to obtain mulberry leaf alkaloid and polysaccharide mixed particles, and b) preparing a liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the proportion of 1: uniformly mixing the components in a volume ratio of 0.1-1 to obtain a slow-release gel coating liquid and c) coating: mixing the mulberry alkaloid and polysaccharide mixed particles prepared in the step a) with a ratio of 1: 10-20 weight-volume ratio into the slow release gel coating liquid prepared in the step b), stirring into suspension and spraying to dry to obtain the finished product. The method can realize continuous control effect on blood sugar, regulate intestinal flora and maintain blood sugar steady state of diabetics.
Description
[ field of technology ]
The invention relates to the technical field of blood sugar regulation, in particular to a mulberry leaf prebiotic for regulating blood sugar steady state based on intestinal probiotics and a preparation method thereof.
[ background Art ]
Blood glucose disorders are metabolic diseases characterized by hyperglycemia, which can lead to chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels or nerves, leading to a series of complications. Research has shown that there is a significant correlation between intestinal microorganisms and the development of diabetes mellitus, and thus regulation of intestinal microorganisms is one of the important means for controlling blood glucose (Chen K, chen H, faas MM, han B, li JH, et al, specific protein-type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrer function, and micro-biological proteins, molecular Nutrition & Food Research,2017,61 (8), 1601006.). Reasonable dietary intervention such as dietary fibers and prebiotics can improve sugar metabolism disorder by regulating intestinal flora, and has the characteristics of safety, economy and convenience (Glenn RG, robert H, mary ES, susan LP, raylene AR, et al Expert consensus document: the International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiological.Nature Reviews Gastroenterology and Hepatology,2017, 14:491-502.). Mulberry leaf is a Chinese medicinal material used as both medicine and food, and is used for treating diabetes (diabetes) in ancient times, and the "herbal schema of Ben Cao" explicitly records the "decoction for replacing tea" of Mulberry leaf, which can stop diabetes. Modern pharmacological studies have found that alkaloids in mulberry leaves can regulate intestinal microorganisms and maintain blood glucose homeostasis (Li Y, ji D, zhong S, lin T, lv Z, hu G, wang X.1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetes mellitus reports,2013;3:1377;Li Y,Zhong S,Yu J,Sun Y,Zhu J,Ji D,Wu C.The mulberry-modified 1-Deoxynojirimycin (DNJ) inhibitors high-fat diet (HFD) -induced hypercholesteremia and modulates the gut microbiota in a gender-specific maner. Journal of Functional Foods,2019; 52:63-72.) however, the short in vivo half-life of mulberry leaves alkaloids affects the sustained control of blood glucose.
[ invention ]
The invention aims to solve the problems in the prior art, and provides a mulberry leaf prebiotic for regulating and controlling blood sugar steady state based on intestinal probiotics and a preparation method thereof.
In order to achieve the above object, the present invention is realized by the following technical scheme:
a preparation method of mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics comprises the following steps:
a) Granulating: mixing folium Mori alkaloid powder and folium Mori polysaccharide powder at a ratio of 1: mixing uniformly in a weight ratio of 0.5-4, adding a solvent, stirring into a bulk or pasty state, and then drying, grinding and sieving to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the proportion of 1: uniformly mixing the components in a volume ratio of 0.1-1 to obtain a slow-release gel coating liquid;
c) Coating: mixing the mulberry alkaloid and polysaccharide mixed particles prepared in the step a) with a ratio of 1: 10-20 weight-volume ratio into the slow release gel coating liquid prepared in the step b), stirring into suspension and spraying to dry to obtain the finished product.
Preferably, in the step a), the mulberry alkaloid powder is prepared by leaching the mulberry alkaloid powder with an organic solvent, volatilizing to remove the organic solvent in the leaching supernatant, adsorbing and eluting with a cation exchange resin, and drying.
Further, in the step a), the step of leaching the mulberry leaf powder with an organic solvent is specifically carried out by mixing the mulberry leaf powder with 1: the mass volume ratio of 30 to 50 is put into 50 to 70 percent of ethanol, and the extraction is carried out for 4 to 6 hours at the temperature of 40 to 60 ℃ and the ultrasonic power of 1000 to 1300W.
Still further, in the step a), the mulberry leaf powder is prepared by picking and chopping fresh mulberry leaves, standing for 1-4 hours at room temperature, naturally drying or drying at 50-60 ℃ by using an oven, crushing again by using a crusher and sieving under 30-80 meshes to obtain the mulberry leaf powder.
Further, in the step a), after leaching is completed, filtering to remove filter residues and collecting filtrate, centrifuging the filtrate at 8000-10000 rpm and collecting supernatant, steaming the supernatant to remove alcohol, adsorbing the supernatant with acid type cation exchange resin for 12-24 h, washing the acid type cation exchange resin with tap water to remove impurities, eluting the acid type cation exchange resin with 0.25-0.5 mol/L ammonia water and collecting eluent, steaming the eluent to remove ammonia water, concentrating to small volume, centrifuging to obtain supernatant, adsorbing, eluting and concentrating the supernatant, and freezing or spray drying to obtain the product.
Preferably, in the step a), the mulberry leaf polysaccharide powder is prepared by first Shui Disang leaf powder, filtering and concentrating the extract, precipitating the concentrate with ethanol, separating by using a dialysis membrane, and drying.
Furthermore, in the step a), the water-extracted mulberry leaf powder is specifically that the feed liquid ratio of the mulberry leaf powder or the mulberry leaf powder residue (according to the weight of the original mulberry leaf powder) after the alkaloid extraction is added into the mulberry leaf powder is 1: 30-50 h of water, and boiling and extracting for 4-6 h. Wherein. The preparation method of the mulberry leaf powder is the same as above.
Further, in the step a), after the water extraction is completed, filtering to remove filter residues and collecting filtrate, centrifuging the filtrate at 8000-10000 rpm and collecting supernatant, concentrating the supernatant to 1/5-1/10 of the original volume, slowly adding ethanol with 2-4 times of the volume of the concentrated solution, centrifuging at 8000-1000 r/min after overnight precipitation, pouring out the supernatant, fully dissolving the precipitate by using hot water to obtain a crude polysaccharide solution, separating the crude polysaccharide solution by using a 10kDa and 100kDa dialysis membrane, performing rotary volatilization concentration to a smaller volume, and then performing freeze drying or spray drying to obtain the polysaccharide.
Preferably, in the step a), the molecular weight of the mulberry polysaccharide in the mulberry polysaccharide powder is 10-100 kDa.
Preferably, in the step a), the ethanol solution with the concentration of 20-50% is added into the uniformly mixed mulberry alkaloid powder and mulberry polysaccharide powder, and the mixture is stirred into a bulk or paste, dried at the temperature of below 60 ℃, ground by a grinder, and sieved by a 30-50-mesh sieve.
Preferably, in the step b), the sericin concentrate is obtained by dissolving sericin powder in water, wherein the sericin powder is prepared by dissolving cocoon shells with alkali liquor, filtering and concentrating the solution, separating with a dialysis membrane, and drying.
Further, in the step b), the solution of the cocoon shells by using the alkali liquor is specifically that the solution ratio of the feed liquid to the cocoon shells is 1: 40-100 mass percent of sodium carbonate solution with the mass percent of 1-5 percent is heated and boiled for 1-3 hours, and is continuously stirred during the boiling period. Wherein the cocoon shell is tussah cocoon shell or silkworm cocoon shell.
Further, in the step b), after the dissolution is finished, filtering to remove filter residues, collecting filtrate, concentrating the filtrate to 1/5-1/10 of the original volume, separating the concentrated solution by using a 10kDa dialysis membrane, and then freeze-drying or spray-drying.
Preferably, in said step b), the molecular weight of the sericin in said sericin concentrate is greater than 10kDa.
Preferably, in the step c), the slow release gel coating liquid is cooled to 50 to 65 ℃.
Preferably, in the step c), the stirring speed is 800 to 1200r/min.
A mulberry leaf prebiotic for regulating blood sugar steady state based on intestinal probiotics is prepared by the preparation method.
The invention has the beneficial effects that:
according to the invention, mulberry leaves and natural silks which are used as medicinal and edible plant parts are taken as main raw materials, mulberry leaf alkaloids and mulberry leaf polysaccharide which has the functions of resisting oxidization, reducing blood sugar and the like and can play a synergistic effect together with the mulberry leaf alkaloids to maintain blood sugar steady state are firstly subjected to gradient separation, adhesive macromolecular sericin which is used as a good functional slow-release matrix and has physical characteristics of water absorption, gelation, modification and the like in silkworm cocoons is further extracted, the synergistic ratio of the mulberry leaf alkaloids and the mulberry leaf polysaccharide in mulberry leaves is optimized and compounded to prepare mulberry leaf alkaloids and polysaccharide mixed particles, and the mulberry leaf alkaloids and polysaccharide mixed particles are embedded in a slow-release gel outer membrane formed by physical crosslinking of the sericin and the hydroxypropyl methylcellulose to finally prepare the mulberry leaf prebiotics which have high naturalness and slow release effects and can regulate and control intestinal flora, so that the continuous control effect of the mulberry leaf alkaloids on blood sugar steady state of diabetics is improved.
The features and advantages of the present invention will be described in detail by way of example with reference to the accompanying drawings.
[ description of the drawings ]
FIG. 1 is a graph comparing the effect of mulberry leaf prebiotics produced in accordance with the present invention, based on intestinal probiotics for regulating glycemic homeostasis, on glucose tolerance in mice;
figure 2 is an effect of mulberry leaf prebiotics produced in accordance with the present invention to regulate blood glucose homeostasis based on intestinal probiotics on the diversity of mice intestinal microorganisms.
In fig. 2, a and b are the effects on α diversity, the effects of the reaction on species abundance; c is the effect on β diversity, the effect of the reaction on flora structure.
[ detailed description ] of the invention
Example one, preparation of mulberry leaf prebiotics for regulating glycemic homeostasis based on intestinal probiotics:
a preparation method of mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics comprises the following steps:
a) Granulating:
a1 Preparation of mulberry leaf powder: picking and chopping fresh mulberry leaves 5 to 10 months each year, standing for 1 to 4 hours at room temperature to ensure that milk of the mulberry leaves overflows fully, naturally drying in the sun or drying in a baking oven at 60 ℃, crushing again by a crusher and sieving under 30 to 80 meshes to obtain mulberry leaf powder;
a2 Preparation of mulberry leaf alkaloid powder: mixing mulberry leaf powder with 1: extracting with 50-70% ethanol at 40-60deg.C under ultrasonic power of 1000-1300W for 4-6 hr, filtering to remove residue after leaching, collecting filtrate, centrifuging at 8000-10000 rpm, collecting supernatant, spin-evaporating supernatant to remove ethanol, adsorbing with acid cation exchange resin for 12-24 hr, washing with acid cation exchange resin with tap water to remove impurities, eluting with 0.25-0.5 mol/L ammonia water, collecting eluate, spin-evaporating eluate to remove ammonia water, concentrating to small volume, centrifuging to obtain supernatant, adsorbing, eluting, concentrating, and freeze-drying or spray-drying to obtain folium Mori alkaloid powder;
a3 Preparation of mulberry leaf polysaccharide powder: the mulberry leaf powder prepared in the step a 1) or the mulberry leaf powder residue (according to the weight of the original mulberry leaf powder) after extracting alkaloid is added with the feed liquid ratio of 1: 30-50 of water, boiling and extracting for 4-6 h, filtering and removing filter residues after water extraction is finished, collecting filtrate, centrifuging the filtrate at 8000-10000 rpm, collecting supernatant, concentrating the supernatant to 1/5-1/10 of the original volume, slowly adding 3 times of ethanol of the concentrated solution, centrifuging at 8000-1000 r/min after precipitation overnight, pouring out the supernatant, fully dissolving the precipitate by using hot water to obtain a crude polysaccharide solution, separating mulberry leaf polysaccharide with the molecular weight of 10-100 kDa in the crude polysaccharide solution by using a 10kDa and 100kDa dialysis membrane, concentrating the crude polysaccharide solution to a smaller volume by rotary volatilization, and then performing freeze drying or spray drying to obtain mulberry leaf polysaccharide powder;
a4 Preparation of mixed particles of mulberry alkaloid and polysaccharide: mixing the mulberry alkaloid powder prepared in the step a 2) and the mulberry polysaccharide powder prepared in the step a 3) with 1: mixing evenly in a weight ratio of 0.5-4, adding ethanol solution with the concentration of 20-50% and stirring into a bulk or paste, drying at the temperature below 60 ℃, grinding by using a grinder, and sieving by a 40-mesh sieve to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid:
b1 Preparation of sericin powder: the feed liquid ratio of the feed liquid is 1 in the silkworm cocoon shell (tussah cocoon shell or silkworm cocoon shell): 40-100 mass percent of sodium carbonate solution with the mass percent of 1-5 percent is heated and boiled for 2 hours, the mixture is continuously stirred during the boiling, filter residues are removed, filtrate is collected, the filtrate is concentrated to 1/5-1/10 of the original volume, sericin with the molecular weight of more than 10kDa in concentrated solution is separated by using a 10kDa dialysis membrane, and then the sericin powder is obtained by freeze drying or spray drying;
b2 A) of: preparation of sericin concentrate: dissolving the sericin powder prepared in the step b 1) in hot water to prepare sericin concentrated solution with the concentration of 50-100 g/L;
b3 Preparation of hypromellose E50 solution: dissolving hydroxypropyl methylcellulose E50 into hot water to prepare a hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5%;
b4 Preparation of a sustained-release gel coating liquid: mixing 50-100 g/L sericin concentrated solution prepared in the step b 2) and 2-5% hydroxypropyl methylcellulose E50 solution prepared in the step b 3) according to the mass percentage of 1: uniformly mixing the components in a volume ratio of 0.1-1, and rapidly stirring the components in a stirrer at a speed of 1000r/min to obtain a slow-release gel coating liquid;
c) Coating: mixing the mulberry alkaloid and polysaccharide mixed particles prepared in the step a 4) with 1: 10-20 weight-volume ratio is added into the slow release gel coating liquid which is prepared in the step b 4) and cooled to 50-65 ℃ and is stirred into suspension at 800-1200 r/min and sprayed to dryness, thus obtaining the finished product.
Example two in vivo evaluation test of mulberry leaf prebiotics for regulating blood glucose homeostasis based on intestinal probiotics:
experimental animals and feeding conditions: 30 clean female ICR mice (Hangzhou son laboratory animal science, inc. [ production license: SCXK (Zhe) 2019-0004 ]) weighing 18-20 g. Barrier system laboratory, temperature: 25+/-1 ℃; humidity: 50-70%; illumination: 150-200 Lx,12h of light and shade alternation; noise <50dB. And (3) drinking water: tap water. The feeding mode is as follows: free diet, mice were fed with sufficient feed and water in mice cages, with 10 mice fed per cage.
Test design and measurement index: mice were randomized into 3 groups of 10 animals each, with the control group given normal diet, the diabetic model group and the prebiotic intervention group given high fat high sugar diet for 8 weeks and finally for 1 week with continuous injection of Streptozotocin (STZ) at a dose of 40mg/kg body weight for 5 days. The prebiotic intervention group is filled with equal volume of ultrapure water for the stomach of the control group and the model group. After 8 weeks, the mice were fasted without water for 12 hours and then blood was taken from the tail vein, and the fasting blood glucose values of the mice were measured by OneTouchR Ultra-glucose meter. The different groups were given ultrapure water by gastric lavage or the mulberry leaf prebiotics prepared by the process were given 0.3g/ml sucrose solution (prepared with physiological saline) 0.1ml/10g immediately orally after 30min, and blood glucose was measured 30min, 60min and 120min after sugar administration. The micro-organisms were subjected to 16SrRNA gene sequencing analysis from the cecal content of the mice.
Data analysis: statistical analysis was performed using SPSS16 software, all data were in mean.+ -. Standard deviation
The test results were evaluated by t-test.
The results show that the mulberry leaf prebiotics prepared by the process obviously improve the glucose tolerance of the diabetic mice (figure 1), control the postprandial blood glucose steady state, improve the diversity of intestinal microorganisms of the diabetic mice (figure 2) and promote the flora structure of the diabetic mice to be normal (figure 2). The results show that the mulberry leaf prebiotics produced by the process can regulate intestinal flora of diabetic mice and control blood sugar steady state.
The above embodiments are illustrative of the present invention, and not limiting, and any simple modifications of the present invention fall within the scope of the present invention.
Claims (10)
1. The preparation method of the mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics is characterized by comprising the following steps:
a) Granulating: mixing folium Mori alkaloid powder and folium Mori polysaccharide powder at a ratio of 1: mixing uniformly in a weight ratio of 0.5-4, adding a solvent, stirring into a bulk or pasty state, and then drying, grinding and sieving to obtain mulberry leaf alkaloid and polysaccharide mixed particles;
b) Preparing liquid: sericin concentrated solution with the concentration of 50-100 g/L and hydroxypropyl methylcellulose E50 solution with the mass fraction of 2-5% are mixed according to the proportion of 1: uniformly mixing the components in a volume ratio of 0.1-1 to obtain a slow-release gel coating liquid;
c) Coating: mixing the mulberry alkaloid and polysaccharide mixed particles prepared in the step a) with a ratio of 1: 10-20 weight-volume ratio into the slow release gel coating liquid prepared in the step b), stirring into suspension and spraying to dry to obtain the finished product.
2. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 1, wherein the method comprises the following steps: in the step a), the mulberry alkaloid powder is prepared by leaching mulberry leaf powder by using an organic solvent, volatilizing to remove the organic solvent in the leaching supernatant, adsorbing and eluting by using cation exchange resin, and drying.
3. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 2, which is characterized in that: in the step a), the step of leaching the mulberry leaf powder by using the organic solvent comprises the following steps of: the mass volume ratio of 30 to 50 is put into 50 to 70 percent of ethanol, and the extraction is carried out for 4 to 6 hours at the temperature of 40 to 60 ℃ and the ultrasonic power of 1000 to 1300W.
4. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 1, wherein the method comprises the following steps: in the step a), the mulberry leaf polysaccharide powder is prepared by the steps of Shui Disang leaf powder, filtering and concentrating the extracting solution, precipitating the concentrated solution with alcohol, separating by using a dialysis membrane, and drying.
5. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 4, wherein the method comprises the following steps: in the step a), the water extraction of the mulberry leaf powder is specifically that the feed liquid ratio of the mulberry leaf powder or the mulberry leaf powder residue after alkaloid extraction is 1: 30-50 h of water, and boiling and extracting for 4-6 h.
6. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 1, wherein the method comprises the following steps: in the step a), the molecular weight of the mulberry leaf polysaccharide in the mulberry leaf polysaccharide powder is 10-100 kDa.
7. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 1, wherein the method comprises the following steps: in the step b), the sericin concentrated solution is obtained by dissolving sericin powder in water, wherein the sericin powder is prepared by dissolving cocoon shells by using alkali liquor, filtering and concentrating the dissolved solution, separating by using a dialysis membrane, and drying.
8. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 7, wherein the method comprises the following steps: in the step b), the solution of the cocoon shells by using alkali liquor is specifically that the feed liquid ratio of 1: 40-100 mass percent of sodium carbonate solution with the mass percent of 1-5 percent is heated and boiled for 1-3 hours, and is continuously stirred during the boiling period.
9. The method for preparing the mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics as claimed in claim 1, wherein the method comprises the following steps: in said step b), the molecular weight of the sericin in said sericin concentrate is greater than 10kDa.
10. A mulberry leaf prebiotic for regulating blood glucose homeostasis based on intestinal probiotics, produced by the method of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210546709.6A CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210546709.6A CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115252677A CN115252677A (en) | 2022-11-01 |
| CN115252677B true CN115252677B (en) | 2023-05-12 |
Family
ID=83760577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210546709.6A Active CN115252677B (en) | 2022-05-18 | 2022-05-18 | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115252677B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006257000A (en) * | 2005-03-16 | 2006-09-28 | Iwase Cosfa Kk | Sericin derivative and composition containing the same |
| CN1850166A (en) * | 2006-03-08 | 2006-10-25 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
| CN105535112A (en) * | 2015-12-29 | 2016-05-04 | 浙江省农业科学院 | Extraction technology of hypoglycemic medicinal active substances of mulberry leaves and mulberries and formula |
| WO2019013172A1 (en) * | 2017-07-12 | 2019-01-17 | 株式会社 きものブレイン | Health food containing silkworm cocoon-derived ingredient |
-
2022
- 2022-05-18 CN CN202210546709.6A patent/CN115252677B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006257000A (en) * | 2005-03-16 | 2006-09-28 | Iwase Cosfa Kk | Sericin derivative and composition containing the same |
| CN1850166A (en) * | 2006-03-08 | 2006-10-25 | 江苏大学 | Composition of white mulberry leaf blood-sugar-reducing effective components and preparing method |
| CN105535112A (en) * | 2015-12-29 | 2016-05-04 | 浙江省农业科学院 | Extraction technology of hypoglycemic medicinal active substances of mulberry leaves and mulberries and formula |
| WO2019013172A1 (en) * | 2017-07-12 | 2019-01-17 | 株式会社 きものブレイン | Health food containing silkworm cocoon-derived ingredient |
Non-Patent Citations (2)
| Title |
|---|
| 丝胶蛋白的结构、性能及生物医学应用;肖肖 等;化学进展;第29卷(第05期);513-523 * |
| 桑叶有效成分降糖作用研究;玄光善 等;食品科学;第32卷(第07期);323-326 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115252677A (en) | 2022-11-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103719880B (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN107412721B (en) | Blood sugar-reducing bitter gourd polypeptide compound capsule and preparation method thereof | |
| CN112472723B (en) | A product containing sea squirt inner capsule and its preparation method | |
| CN110772630A (en) | Compound bitter gourd peptide oral liquid for activating insulin receptor and regulating blood sugar and preparation method thereof | |
| WO2021042700A1 (en) | Method for extracting hemp polysaccharides, product obtained thereby and use thereof | |
| US20220370541A1 (en) | Activated insulin, compound momordica charantia peptide oral medicine for treatment of diabetes, and preparation method | |
| CN115252677B (en) | Mulberry leaf prebiotics for regulating and controlling blood glucose homeostasis based on intestinal probiotics and preparation method thereof | |
| CN107198010A (en) | A kind of crocodile blood pressed candy | |
| CN105707408A (en) | Preparing method for protein isolate of sacha inchi fruits | |
| CN112655834A (en) | Piglet health-care feed additive composition and application thereof | |
| CN103342755A (en) | Lycium barbarum polysaccharide homogeneous fraction IV, and preparation method and application thereof | |
| CN116035218A (en) | Pea oligopeptide health care product and preparation method thereof | |
| CN110179128A (en) | Compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide and preparation method thereof | |
| CN116369382A (en) | Plant polypeptide nutrition preparation for promoting teenager development and growth and preparation method thereof | |
| CN109645502A (en) | High-quality dietary fiber, the preparation method of honey raisin tree diet fiber composition and product | |
| CN102232561A (en) | Chinese wolfberry cellulose capsule and preparation method thereof | |
| CN114920856A (en) | Method for preparing high-whiteness konjac glucomannan from fine bulbil konjac powder | |
| CN112205621A (en) | Functional food suitable for diabetics | |
| CN114107418A (en) | Preparation method of ginseng polypeptide | |
| CN111789256B (en) | Pitaya flower polysaccharide composition with insulin balancing effect | |
| CN109320626B (en) | Aloe polysaccharide and preparation method and application thereof | |
| CN110973460A (en) | Sugar-reducing fresh rice and processing method thereof | |
| CN117243380A (en) | Blood sugar reducing compound composition, preparation method and application thereof | |
| CN1051910C (en) | Preparing method for bagasse fibre powder food | |
| CN116284488A (en) | Extraction method of Polygonatum kingianum polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |