CN110179128A - Compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide and preparation method thereof - Google Patents
Compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide and preparation method thereof Download PDFInfo
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- CN110179128A CN110179128A CN201910636315.8A CN201910636315A CN110179128A CN 110179128 A CN110179128 A CN 110179128A CN 201910636315 A CN201910636315 A CN 201910636315A CN 110179128 A CN110179128 A CN 110179128A
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- fiber crops
- extract
- chinese fiber
- crops seed
- mulberries
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 111
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 108
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 104
- 239000000835 fiber Substances 0.000 title claims abstract description 98
- 239000002775 capsule Substances 0.000 title claims abstract description 58
- 150000001875 compounds Chemical class 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 241000218231 Moraceae Species 0.000 claims abstract description 75
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 75
- 239000000284 extract Substances 0.000 claims abstract description 73
- 235000004347 Perilla Nutrition 0.000 claims abstract description 47
- 235000020237 cranberry extract Nutrition 0.000 claims abstract description 42
- 229920002472 Starch Polymers 0.000 claims abstract description 30
- 235000019698 starch Nutrition 0.000 claims abstract description 30
- 229920001353 Dextrin Polymers 0.000 claims abstract description 18
- 239000004375 Dextrin Substances 0.000 claims abstract description 18
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 18
- 235000019425 dextrin Nutrition 0.000 claims abstract description 18
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 18
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 18
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 18
- 239000008107 starch Substances 0.000 claims abstract description 18
- 230000000694 effects Effects 0.000 claims abstract description 16
- 238000012545 processing Methods 0.000 claims abstract description 3
- 241000229722 Perilla <angiosperm> Species 0.000 claims description 45
- 239000007788 liquid Substances 0.000 claims description 40
- 238000001035 drying Methods 0.000 claims description 35
- 235000012545 Vaccinium macrocarpon Nutrition 0.000 claims description 31
- 244000291414 Vaccinium oxycoccus Species 0.000 claims description 31
- 235000002118 Vaccinium oxycoccus Nutrition 0.000 claims description 31
- 235000004634 cranberry Nutrition 0.000 claims description 31
- 238000005238 degreasing Methods 0.000 claims description 26
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 24
- 238000012216 screening Methods 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000287 crude extract Substances 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 229940088598 enzyme Drugs 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 235000020985 whole grains Nutrition 0.000 claims description 15
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 claims description 14
- 235000019441 ethanol Nutrition 0.000 claims description 14
- 229920002414 procyanidin Polymers 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 239000003208 petroleum Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- 238000000108 ultra-filtration Methods 0.000 claims description 8
- 108090000526 Papain Proteins 0.000 claims description 7
- 229940055729 papain Drugs 0.000 claims description 7
- 235000019834 papain Nutrition 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 6
- 230000009849 deactivation Effects 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 4
- 238000005550 wet granulation Methods 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 229930014669 anthocyanidin Natural products 0.000 claims description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 2
- 235000008758 anthocyanidins Nutrition 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 239000000479 mixture part Substances 0.000 claims 1
- 235000019419 proteases Nutrition 0.000 claims 1
- 230000036039 immunity Effects 0.000 abstract description 8
- 102000057297 Pepsin A Human genes 0.000 abstract description 5
- 108090000284 Pepsin A Proteins 0.000 abstract description 5
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 229940111202 pepsin Drugs 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 210000003734 kidney Anatomy 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 244000124853 Perilla frutescens Species 0.000 abstract 2
- 238000000034 method Methods 0.000 description 13
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- 238000004806 packaging method and process Methods 0.000 description 9
- 239000002994 raw material Substances 0.000 description 9
- 239000003963 antioxidant agent Substances 0.000 description 4
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- 235000006708 antioxidants Nutrition 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
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- 235000016709 nutrition Nutrition 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000001151 other effect Effects 0.000 description 2
- 230000000242 pagocytic effect Effects 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010012434 Dermatitis allergic Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 241000167880 Hirundinidae Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010048908 Seasonal allergy Diseases 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 201000008937 atopic dermatitis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000003024 peritoneal macrophage Anatomy 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to compound enteric-coated capsules of a kind of Chinese fiber crops seed polypeptide and preparation method thereof, belong to food processing technology field.It cannot play a role to solve the problems, such as that active peptides are directly oral vulnerable to Pepsin degradation, the present invention provides a kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, component includes 3~5 parts of Chinese fiber crops seed polypeptide, 0.1~0.5 part of mulberries extract, 1~3 part of Cranberry extract, 1~3 part of perilla leaf extract, 1~3 part of starch, 0.5~1 part of microcrystalline cellulose and 0.5~1 part of dextrin.The compound enteric-coated capsule of Chinese fiber crops seed polypeptide produced by the present invention, can be avoided degradation of the pepsin to polypeptide, stablizes it in enteron aisle and effectively control discharges, improve the utilization rate of polypeptide；Addition mulberries extract, Cranberry extract, perilla leaf extract play synergistic effect, make the compound enteric-coated capsule of Chinese fiber crops seed polypeptide have effects that keep fit and healthy, enriching yin and nourishing kidney, strengthen immunity.
Description
Technical field
The invention belongs to food processing technology field more particularly to a kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide and its preparations
Method.
Background technique
Modern biotechnology metabolism studies have shown that animal intake protein in enteron aisle after digesting enzyme effect, be largely
It is absorbed in the form of small peptide, small-molecular peptides absorptivity of the relative molecular mass less than 1000 is 90% or more.Therefore, peptide can be with
For maintaining and improving proteinaceous nutrient state, as enteral nutrition agent or fluidised form food is made.In addition, peptide also has drop blood
The multiple efficacies such as rouge, anti-oxidant, raising immunity, play extremely important effect in organism metabolism adjusting.Polypeptide has been at present
It is widely used in food, medicine and other fields.
But when directly oral due to active peptides, vulnerable to the degradation of pepsin, cause polypeptide bioactivity cannot
It gives full play to, seriously affects activity persistency, be restricted its application.
Summary of the invention
It cannot play a role to solve the problems, such as that active peptides are directly oral vulnerable to Pepsin degradation, the present invention provides
A kind of Chinese fiber crops seed polypeptide compound enteric-coated capsule and preparation method thereof.
Technical solution of the present invention:
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3~5 parts of Chinese fiber crops seed polypeptide, mulberries
0.1~0.5 part of extract, 1~3 part of Cranberry extract, 1~3 part of perilla leaf extract, 1~3 part of starch, microcrystalline cellulose
0.5~1 part and 0.5~1 part of dextrin.
Further, the molecular weight of the Chinese fiber crops seed polypeptide is less than 1000Da, and total starches contains in the mulberries extract
Amount is 30~50%, and the content of the Cranberry extract procyanidins is 10~30%.
A kind of preparation method of the compound enteric-coated capsule of Chinese fiber crops seed polypeptide, includes the following steps:
Step 1: preparing Chinese fiber crops seed polypeptide:
The Chinese fiber crops seed dregs of rice of degreasing mixs with water by certain solid-liquid ratio, into gained mixed liquor addition papain with
Neutral proteinase carries out complex enzyme hydrolysis at a certain temperature, is centrifuged after the heating enzyme deactivation of gained enzymolysis liquid, centrifuged supernatant is taken to pass through
1000Da ultrafiltration membrane carries out ultrafiltration, obtains the polypeptide enzymolysis liquid that molecular weight is less than 1000Da, Chinese fiber crops are obtained after frozen drying
Seed polypeptide；
Step 2: preparation mulberries extract:
Mulberries vacuum freeze drying, crushing, sieving are obtained into mulberries freeze-dried powder, by certain mass volume ratio by the mulberries
Freeze-dried powder is added in petroleum ether and carries out ungrease treatment, degreasing mulberries product is obtained, by certain solid-to-liquid ratio into degreasing mulberries product
Add water, water-bath extracts certain time and obtains mulberries crude extract, filtering, centrifugation, after taking centrifuged supernatant to refilter under certain temperature
It is dried to obtain mulberries extract；
Step 3: preparation Cranberry extract:
By Cranberry frozen drying, crush, by certain mass volume ratio by the Cranberry addition petroleum ether in into
Row ungrease treatment obtains degreasing Cranberry freeze-dried powder, and 75% ethyl alcohol is added into degreasing Cranberry freeze-dried powder by certain solid-liquid ratio,
Alcohol extracting is carried out under certain temperature and obtains Cranberry crude extract, and the Cranberry crude extract is filtered, is lyophilized to obtain Cranberry extraction
Object；
Step 4: preparing perilla leaf extract:
Fresh perilla leaf adds water by certain solid-to-liquid ratio, and the extraction certain time of water-bath at a certain temperature obtains perilla leaf and slightly mentions
Liquid, the perilla leaf crude extract centrifuging and taking supernatant liquid filtering, freeze-drying obtain perilla leaf extract；
Step 5: preparing the compound enteric-coated capsule of Chinese fiber crops seed polypeptide:
3~5 parts of Chinese fiber crops seed polypeptide, 0.1~0.5 part of mulberries extract, 1~3 part of Cranberry extract and perilla leaf is taken to mention
It takes 1~3 part of object to be sufficiently mixed, 1~3 part of starch, 0.5~1 part of microcrystalline cellulose and dextrin is added into gained mixture
0.5~1 part, drying and screening, wet granulation, drying and screening whole grain, irradiation sterilization, loading capsulae enterosolubilis obtain after mixing
The compound enteric-coated capsule of Chinese fiber crops seed polypeptide.
Further, solid-liquid ratio described in step 1 is 1g:10g；The enzyme activity of the papain is 800,000 u/g, is added
Amount is the 0.2~0.6% of mixed liquor quality；The enzyme activity of the neutral proteinase is 130,000 u/g, additional amount is mixed liquor quality
2.0~3.0%.
Further, hydrolysis temperature described in step 1 is 60~70 DEG C, and enzymolysis time is 1~3h, and the heating enzyme deactivation is
Enzymolysis liquid is placed in 90~100 DEG C of 10~20min of heating.
Further, sieving described in step 2 is 80 meshes；The mass volume ratio of the mulberries freeze-dried powder and petroleum ether is
1g:5mL, the degreasing time are 90min；The solid-to-liquid ratio is 1g:15mL, and the bath temperature is 80 DEG C, and extraction time is
5h。
Further, crude extract described in step 2 first uses 4 layers of cotton gauze filtering, and filtered solution 8000r/min is centrifuged, is centrifuged
Time is 20min；It takes centrifuged supernatant to cross 300 mesh filter clothes, is dried to obtain mulberries extract, polysaccharide in the mulberries extract
Content is 30~50%.
Further, the mass volume ratio of Cranberry and petroleum ether described in step 3 is 1g:5mL, and the degreasing time is
90min；The solid-liquid ratio of the degreasing Cranberry freeze-dried powder and 75% ethyl alcohol is 1g:15mL；The alcohol extracting temperature is 60 DEG C, alcohol extracting
Time is 1h；The content of the Cranberry extract procyanidins is 10~30%.
Further, the solid-to-liquid ratio of fresh perilla leaf and water described in step 4 is 1g:10mL；The bath temperature be 60~
70 DEG C, the extraction time is 2h, and the centrifugal rotational speed is 8000r/min, centrifugation time 20min；Filter cloth is used in the filtering
For 100 mesh filter clothes.
Further, drying temperature described in step 5 is 50~70 DEG C；The sieving whole grain was 30 mesh mesh screens；The spoke
It is ultraviolet irradiation 30min according to sterilizing.
Beneficial effects of the present invention:
The present invention, which prepares Chinese fiber crops seed polypeptide, has anti-oxidant, hypoglycemic, reducing blood lipid, raising immunity and other effects, furthermore the Chinese
Arginine content in numb seed polypeptide is higher, and smart amino acid content is 93.4mg/g, and arginine has the growth and development of children
Important role.Chinese fiber crops polypeptide molecular weight in the present invention is no more than 1000Da, it is easier to be absorbed by the body.
The nutritional ingredients such as amino acid rich in, vitamin, minerals, also contain in mulberries extract prepared by the present invention
There are flavones, polysaccharide isoreactivity ingredient.Wherein mulberries polysaccharide, which has, adjusts immunity, anti-oxidant, hypoglycemic, antifatigue and other effects.
Cranberry extract procyanidine rich in (34.3mg/100g) prepared by the present invention, procyanidine is one
Kind there are the natural polyphenol of nature, have that anti-oxidant, protection is cardiovascular, anti-inflammatory, antibacterial, antitumor and immunological regulation etc. are biological
Activity.
Perilla leaf extract prepared by the present invention has relieving exterior syndrome and dispelling cold, relieving QI stagnancy in the stomach, relax bowel and defecation, the function such as preventing phlegm from forming and stopping coughing
Effect.Perilla leaf vitamin C rich in, potassium, iron etc., unsaturated fatty acid also rich in, having makes immune function just
The effect often played has and mitigates the anaphylactoid effects such as allergic dermatitis, pollinosis.Part acid energy in perilla leaf
Enough pass through alleviates smooth muscle spasm, and plays the role of relievining asthma.
Prepared Chinese fiber crops seed polypeptide, mulberries extract, Cranberry extract and perilla leaf extract are made the present invention
The compound enteric-coated capsule of Chinese fiber crops seed polypeptide, can be avoided degradation of the pepsin to polypeptide, enable contained active constituent in enteron aisle
Middle stabilization and effectively control release, the effect of improving the utilization efficiency of Chinese fiber crops seed polypeptide, give full play to Chinese fiber crops seed polypeptide；Addition
Mulberries extract, Cranberry extract, perilla leaf extract further improve nutrition and health care effect, make Chinese fiber crops seed polypeptide with
Mulberries polysaccharide, Cranberry anthocyanidin, perilla leaf play synergistic effect, have the compound enteric-coated capsule of Chinese fiber crops seed polypeptide not only
There are reducing blood lipid, hypoglycemic effect, moreover it is possible to keep fit and healthy, enriching yin and nourishing kidney, strengthen immunity.
The compound enteric-coated capsule of Chinese fiber crops seed polypeptide prepared by the present invention stores, is edible, easy to carry, has expanded Chinese fiber crops seed polypeptide
Application in health food, medicine and other fields.
Specific embodiment
Below with reference to embodiment, the following further describes the technical solution of the present invention, and however, it is not limited to this, all right
Technical solution of the present invention is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be contained
Lid is within the protection scope of the present invention.
Embodiment 1
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3~5 parts of Chinese fiber crops seed polypeptide, mulberries
0.1~0.5 part of extract, 1~3 part of Cranberry extract, 1~3 part of perilla leaf extract, 1~3 part of starch, microcrystalline cellulose
0.5~1 part and 0.5~1 part of dextrin.
Embodiment 2
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3~5 parts of Chinese fiber crops seed polypeptide, mulberries
0.1~0.5 part of extract, 1~3 part of Cranberry extract, 1~3 part of perilla leaf extract, 1~3 part of starch, microcrystalline cellulose
0.5~1 part and 0.5~1 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
Embodiment 3
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.1 part of object, 1 part of Cranberry extract, 1 part of perilla leaf extract, 3 parts of starch, 0.5 part of microcrystalline cellulose and 0.5 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 4
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.3 part of object, 2 parts of Cranberry extract, 2 parts of perilla leaf extract, 3 parts of starch, 0.5 part of microcrystalline cellulose and 0.5 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 5
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 3 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.5 part of object, 3 parts of Cranberry extract, 3 parts of perilla leaf extract, 3 parts of starch, 0.5 part of microcrystalline cellulose and 0.5 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 6
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 4 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.1 part of object, 2 parts of Cranberry extract, 3 parts of perilla leaf extract, 2 parts of starch, 0.75 part of microcrystalline cellulose and dextrin 0.75
Part.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 7
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 4 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.3 part of object, 3 parts of Cranberry extract, 1 part of perilla leaf extract, 2 parts of starch, 0.75 part of microcrystalline cellulose and dextrin 0.75
Part.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 8
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 4 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.5 part of object, 1 part of Cranberry extract, 2 parts of perilla leaf extract, 2 parts of starch, 0.75 part of microcrystalline cellulose and dextrin 0.75
Part.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 9
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 5 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.1 part of object, 3 parts of Cranberry extract, 2 parts of perilla leaf extract, 1 part of starch, 1 part of microcrystalline cellulose and 1 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 10
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 5 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.3 part of object, 1 part of Cranberry extract, 3 parts of perilla leaf extract, 1 part of starch, 1 part of microcrystalline cellulose and 1 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 11
A kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, the component including following parts by weight: 5 parts of Chinese fiber crops seed polypeptide, mulberries mention
Take 0.5 part of object, 2 parts of Cranberry extract, 1 part of perilla leaf extract, 1 part of starch, 1 part of microcrystalline cellulose and 1 part of dextrin.
The molecular weight of Chinese fiber crops seed polypeptide is less than 1000Da in the present embodiment, in mulberries extract the content of total starches be 30~
50%, the content of Cranberry extract procyanidins is 10~30%.
The present embodiment preparation method includes: drying and screening after mixing each raw material of above-mentioned formula ratio with auxiliary material, using wet
Method granulation, drying and screening whole grain, ultraviolet sterilization, filling capsule, packaging are finished product.
Embodiment 12
The preparation method for present embodiments providing a kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, includes the following steps:
Step 1: preparing Chinese fiber crops seed polypeptide:
The Chinese fiber crops seed dregs of rice of degreasing mixs with water by certain solid-liquid ratio, into gained mixed liquor addition papain with
Neutral proteinase carries out complex enzyme hydrolysis at a certain temperature, is centrifuged after the heating enzyme deactivation of gained enzymolysis liquid, centrifuged supernatant is taken to pass through
1000Da ultrafiltration membrane carries out ultrafiltration, obtains the polypeptide enzymolysis liquid that molecular weight is less than 1000Da, Chinese fiber crops are obtained after frozen drying
Seed polypeptide；
Step 2: preparation mulberries extract:
Mulberries vacuum freeze drying, crushing, sieving are obtained into mulberries freeze-dried powder, by certain mass volume ratio by the mulberries
Freeze-dried powder is added in petroleum ether and carries out ungrease treatment, degreasing mulberries product is obtained, by certain solid-to-liquid ratio into degreasing mulberries product
Add water, water-bath extracts certain time and obtains mulberries crude extract, filtering, centrifugation, after taking centrifuged supernatant to refilter under certain temperature
It is dried to obtain mulberries extract；
Step 3: preparation Cranberry extract:
By Cranberry frozen drying, crush, by certain mass volume ratio by the Cranberry addition petroleum ether in into
Row ungrease treatment obtains degreasing Cranberry freeze-dried powder, and 75% ethyl alcohol is added into degreasing Cranberry freeze-dried powder by certain solid-liquid ratio,
Alcohol extracting obtains Cranberry crude extract under certain temperature, and the Cranberry crude extract is filtered, is lyophilized to obtain Cranberry extract；
Step 4: preparing perilla leaf extract:
Fresh perilla leaf adds water by certain solid-to-liquid ratio, and the extraction certain time of water-bath at a certain temperature obtains perilla leaf and slightly mentions
Liquid, the perilla leaf crude extract centrifuging and taking supernatant liquid filtering, freeze-drying obtain perilla leaf extract；
Step 5: preparing the compound enteric-coated capsule of Chinese fiber crops seed polypeptide:
3~5 parts of Chinese fiber crops seed polypeptide, 0.1~0.5 part of mulberries extract, 1~3 part of Cranberry extract and perilla leaf is taken to mention
It takes 1~3 part of object to be sufficiently mixed, 1~3 part of starch, 0.5~1 part of microcrystalline cellulose and dextrin is added into gained mixture
0.5~1 part, drying and screening, wet granulation, drying and screening whole grain, irradiation sterilization, loading capsulae enterosolubilis obtain after mixing
The compound enteric-coated capsule of Chinese fiber crops seed polypeptide.
Embodiment 13
The preparation method for present embodiments providing a kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, includes the following steps:
Step 1: preparing Chinese fiber crops seed polypeptide:
The Chinese fiber crops seed dregs of rice of degreasing are mixed by solid-liquid ratio 1g:10g with water, it is 800,000 u/ that enzyme activity is added into gained mixed liquor
The papain and enzyme activity of g is the neutral proteinase of 130,000 u/g, and wherein the additional amount of papain is mixed liquor quality
0.2~0.6%, the additional amount of neutral proteinase carries out compound at a temperature of being 2.0~3.0%, 60~70 DEG C of mixed liquor quality
1~3h is digested, gained enzymolysis liquid is centrifuged after being placed in 90~100 DEG C of 10~20min of heating heating enzyme deactivations, and centrifuged supernatant is taken to pass through
1000Da ultrafiltration membrane carries out ultrafiltration, obtains the polypeptide enzymolysis liquid that molecular weight is less than 1000Da, Chinese fiber crops are obtained after frozen drying
Seed polypeptide；
Step 2: preparation mulberries extract:
Mulberries vacuum freeze drying, crushing, 80 meshes excessively are obtained into mulberries freeze-dried powder, it will by the mass volume ratio of 1g:5mL
Ungrease treatment 90min in petroleum ether is added in the mulberries freeze-dried powder, obtains degreasing mulberries product, by 1g:15mL solid-to-liquid ratio to
Add water in degreasing mulberries product, water-bath extracts 5h and obtains mulberries crude extract at a temperature of 80 DEG C；Crude extract first uses 4 layers of cotton gauze mistake
Filter, filtered solution 8000r/min is centrifuged, centrifugation time 20min；It takes centrifuged supernatant to cross 300 mesh filter clothes, is dried to obtain mulberries
Extract, the content of polysaccharide is 30~50% in the mulberries extract；
Step 3: preparation Cranberry extract:
It by Cranberry frozen drying, crushes, petroleum ether is added in the Cranberry by the mass volume ratio of 1g:5mL
Middle ungrease treatment 90min obtains degreasing Cranberry freeze-dried powder, is added by 1g:15mL solid-liquid ratio into degreasing Cranberry freeze-dried powder
75% ethyl alcohol, alcohol extracting 1h obtains Cranberry crude extract at a temperature of 60 DEG C, the Cranberry crude extract filtered, be lyophilized to obtain it is climing more
Certain kind of berries extract, the content of Cranberry extract procyanidins are 10~30%；
Step 4: preparing perilla leaf extract:
Fresh perilla leaf adds water by 1g:10mL solid-to-liquid ratio, and it is thick to obtain perilla leaf by water-bath extraction 2h at a temperature of 60~70 DEG C
Extract, the perilla leaf crude extract 8000r/min, centrifugation time 20min take supernatant with 100 mesh filter-cloth filterings, are lyophilized
To perilla leaf extract；
Step 5: preparing the compound enteric-coated capsule of Chinese fiber crops seed polypeptide:
Take 5 parts of Chinese fiber crops seed polypeptide, 0.5 part of mulberries extract, 2 parts of Cranberry extract and 1 part of perilla leaf extract progress
It is sufficiently mixed, 1 part of starch, 1 part of microcrystalline cellulose and 1 part of dextrin is added into gained mixture, after mixing dried 100
Mesh, wet granulation, 50~70 DEG C of dryings cross 30 mesh mesh screen whole grains, ultraviolet irradiation 30min sterilizing, are packed into capsulae enterosolubilis to obtain the final product
To the compound enteric-coated capsule of Chinese fiber crops seed polypeptide.
Verify the compound enteric-coated capsule strengthen immunity functional verification test of Chinese fiber crops seed polypeptide manufactured in the present embodiment:
According to the test side of strengthen immunity function in " health food is examined and assessment technique specification " (Ministry of Public Health 2003)
Method, by the compound enteric-coated capsule of Chinese fiber crops seed polypeptide manufactured in the present embodiment press human body recommend 5 times, 10 times and 20 times of dosage (i.e.
0.2g/kg.bw, 0.4g/kg.bw and 0.8g/kg.bw) experiment mice is given in three dosage stomach-fillings, while setting control group (distillation
Water), 1 time a day, continuous gavage 30d, test result is as shown in table 1:
Table 1
As shown in Table 1 the compound enteric-coated capsule of Chinese fiber crops seed polypeptide to mouse chicken red blood cell phagocytic function test the result shows that, respectively
Dosage group mouse chicken red blood cell phagocytic index is above control group, and the compound enteric-coated capsule of Chinese fiber crops seed polypeptide is remarkably improved mouse
Peritoneal macrophage swallows the ability of chicken red blood cell, this indicates that the compound enteric-coated capsule of Chinese fiber crops seed polypeptide has the function of strengthen immunity
Effect.
Claims (10)
1. a kind of compound enteric-coated capsule of Chinese fiber crops seed polypeptide, it is characterised in that: the component including following parts by weight: Chinese fiber crops seed polypeptide 3
~5 parts, 0.1~0.5 part of mulberries extract, 1~3 part of Cranberry extract, 1~3 part of perilla leaf extract, 1~3 part of starch,
0.5~1 part and 0.5~1 part of dextrin of microcrystalline cellulose.
2. the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 1, it is characterised in that: the Chinese fiber crops seed polypeptide
Molecular weight is less than 1000Da, and the content of total starches is 30~50% in the mulberries extract, the Cranberry extract Central Plains
The content of anthocyanidin is 10~30%.
3. a kind of preparation method of the compound enteric-coated capsule of Chinese fiber crops seed polypeptide, characterized by the following steps:
Step 1: preparing Chinese fiber crops seed polypeptide:
The Chinese fiber crops seed dregs of rice of degreasing are mixed by certain solid-liquid ratio with water, papain and neutrality are added into gained mixed liquor
Protease carries out complex enzyme hydrolysis at a certain temperature, is centrifuged after the heating enzyme deactivation of gained enzymolysis liquid, takes centrifuged supernatant through 1000Da
Ultrafiltration membrane carries out ultrafiltration, obtains the polypeptide enzymolysis liquid that molecular weight is less than 1000Da, obtains Chinese fiber crops seed polypeptide after frozen drying；
Step 2: preparation mulberries extract:
Mulberries vacuum freeze drying, crushing, sieving are obtained into mulberries freeze-dried powder, the mulberries are lyophilized by certain mass volume ratio
Powder is added in petroleum ether and carries out ungrease treatment, obtains degreasing mulberries product, adds water into degreasing mulberries product by certain solid-to-liquid ratio,
Water-bath extracts certain time and obtains mulberries crude extract under certain temperature, filtering, centrifugation, dry after taking centrifuged supernatant to refilter
To mulberries extract；
Step 3: preparation Cranberry extract:
It by Cranberry frozen drying, crushes, the Cranberry is added in petroleum ether by certain mass volume ratio and takes off
Rouge processing, obtains degreasing Cranberry freeze-dried powder, 75% ethyl alcohol is added into degreasing Cranberry freeze-dried powder by certain solid-liquid ratio, centainly
At a temperature of carry out alcohol extracting obtain Cranberry crude extract, the Cranberry crude extract is filtered, is lyophilized to obtain Cranberry extract；
Step 4: preparing perilla leaf extract:
Fresh perilla leaf adds water by certain solid-to-liquid ratio, and water-bath at a certain temperature extracts certain time and obtains perilla leaf crude extract,
The perilla leaf crude extract centrifuging and taking supernatant liquid filtering, freeze-drying obtain perilla leaf extract；
Step 5: preparing the compound enteric-coated capsule of Chinese fiber crops seed polypeptide:
Take 3~5 parts of Chinese fiber crops seed polypeptide, 0.1~0.5 part of mulberries extract, 1~3 part of Cranberry extract and perilla leaf extract 1
~3 parts are sufficiently mixed, and 1~3 part of starch, 0.5~1 part of microcrystalline cellulose and dextrin 0.5~1 are added into gained mixture
Part, drying and screening, wet granulation, drying and screening whole grain, irradiation sterilization, loading capsulae enterosolubilis obtain Chinese fiber crops seed after mixing
The compound enteric-coated capsule of polypeptide.
4. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 3, it is characterised in that: step 1
The solid-liquid ratio is 1g:10g；The enzyme activity of the papain is 800,000 u/g, additional amount is mixed liquor quality 0.2~
0.6%；The enzyme activity of the neutral proteinase is 130,000 u/g, additional amount is mixed liquor quality 2.0~3.0%.
5. according to a kind of preparation method of the compound enteric-coated capsule of the Chinese fiber crops seed polypeptide of claim 3 or 4, it is characterised in that: step
A rapid hydrolysis temperature is 60~70 DEG C, and enzymolysis time is 1~3h, and the heating enzyme deactivation is that enzymolysis liquid is placed in 90~100
DEG C heating 10~20min.
6. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 5, it is characterised in that: step 2
The sieving is 80 meshes；The mass volume ratio of the mulberries freeze-dried powder and petroleum ether is 1g:5mL, and the degreasing time is
90min；The solid-to-liquid ratio is 1g:15mL, and the bath temperature is 80 DEG C, extraction time 5h.
7. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 6, it is characterised in that: step 2
The crude extract first uses 4 layers of cotton gauze filtering, filtered solution 8000r/min is centrifuged, centrifugation time 20min；Take centrifugation supernatant
Liquid crosses 300 mesh filter clothes, is dried to obtain mulberries extract, and the content of polysaccharide is 30~50% in the mulberries extract.
8. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 7, it is characterised in that: step 3
The mass volume ratio of the Cranberry and petroleum ether is 1g:5mL, and the degreasing time is 90min；The degreasing Cranberry freeze-drying
The solid-liquid ratio of powder and 75% ethyl alcohol is 1g:15mL；The alcohol extracting temperature is 60 DEG C, and the alcohol extracting time is 1h；The Cranberry is extracted
The content of object procyanidins is 10~30%.
9. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 8, it is characterised in that: step 4
The solid-to-liquid ratio of the fresh perilla leaf and water is 1g:10mL；The bath temperature is 60~70 DEG C, and the extraction time is 2h,
The centrifugal rotational speed is 8000r/min, centrifugation time 20min；Filtering filter cloth is 100 mesh filter clothes.
10. the preparation method of the compound enteric-coated capsule of a kind of Chinese fiber crops seed polypeptide according to claim 9, it is characterised in that: step
Five drying temperatures are 50~70 DEG C；The sieving whole grain was 30 mesh mesh screens；The irradiation sterilization is ultraviolet irradiation
30min。
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Application publication date: 20190830 |
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