CN116035218A - Pea oligopeptide health care product and preparation method thereof - Google Patents
Pea oligopeptide health care product and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention relates to the technical field of health products, and discloses a health product of pea oligopeptide, which comprises the following main components in percentage by mass: 40-120 parts of pea peptide powder, 10-30 parts of earthworm natural polypeptide powder, 30-90 parts of earthworm hydrolyzed polypeptide powder, 8-12 parts of bone meal, 5-10 parts of chitosan, 10-15 parts of concentrated whey protein powder, 6-9 parts of turtle shell, 1-3 parts of ginseng powder and 0.5-1.5 parts of calcium carbonate are sequentially mixed into sugar powder according to mass, and the sugar powder is pressed into slices by a press to prepare health-care product slices, and by arranging earthworm peptide and pea peptide in raw materials, all essential amino acids of a human body can be provided uniformly by adding animal and plant double proteins, and by replacing proteins with small aggregated earthworm peptide and pea peptide, on one hand, the front and back effects are consistent, on the other hand, the earthworm peptide is more convenient to absorb, and by using the mixture of natural polypeptide and hydrolyzed polypeptide of the earthworm peptide, the product can have the characteristics of the natural polypeptide and hydrolyzed polypeptide at the same time, and the effect of the product is improved.
Description
Technical Field
The invention relates to the technical field of health products, in particular to a health product of pea oligopeptide and a preparation method thereof.
Background
The aging phenomenon of the population in China is continuously aggravated, and the nutrition condition of the old influences the health condition, so that the old is helped to improve the health condition by taking high-quality protein, unsaturated fatty acid and trace nutrients, and the nutrition health-care food has great meaning to the old. Middle-aged and elderly people are also plagued by insufficient calcium intake and reduced bioavailability, and simultaneously, the synthesis of parathyroid hormone and vitamin D for regulating and controlling calcium metabolism is insufficient, so that the old people are more likely to lack calcium, and osteoporosis and fracture are likely to be caused; therefore, the elderly are vital to ingest high quality proteins in time.
The animal protein contains 8 amino acids necessary for human body, and has good absorption efficiency. The essential amino acid content of the plant protein is rich, and the cholesterol in the body is reduced, so that animal and plant double proteins are added, and all essential amino acids of a human body can be provided in a balanced way.
The existing health care products usually select macromolecular proteins when being added, and meet the requirements of human bodies on the proteins, but the macromolecular proteins have low absorption rate when being absorbed and cannot be absorbed by human bodies quickly, and the structures of the polypeptides and the proteins are the same and are all composed of amino acids, so that analysis is carried out from the aspect of amino acid nutrition, and the polypeptides and the proteins are the same. However, polypeptides are much smaller in molecular weight than proteins and have physiological regulatory functions that some proteins do not. As basic nutrient substances, the polypeptide is easier to absorb than amino acid, and has high bioavailability. Certain low molecular peptides have the effects of preventing and treating diseases and regulating physiological functions of human bodies, and the effects are not possessed by original proteins and amino acids formed by the original proteins.
Therefore, it is necessary to provide a health care product of pea oligopeptide and a preparation method thereof to solve the technical problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides a health care product of pea oligopeptide and a preparation method thereof.
The health care product of pea oligopeptide provided by the invention comprises the following main components in percentage by mass: 40-120 parts of pea peptide powder, 40-120 parts of earthworm peptide powder, 8-12 parts of bone powder, 5-10 parts of chitosan, 10-15 parts of concentrated whey protein powder, 6-9 parts of turtle shell, 1-3 parts of ginseng powder and 0.5-1.5 parts of calcium carbonate.
Preferably, the earthworm peptide powder consists of 10-30 parts of earthworm natural polypeptide powder and 30-90 parts of earthworm hydrolyzed polypeptide powder, and pea peptide with molecular weight below 3000 daltons is selected as the pea peptide powder.
The specific preparation method of the health product comprises the following steps:
1. crushing raw materials: sterilizing bone, soft-shelled turtle shell and ginseng, quick-freezing by a freezing device, pulverizing the quick-frozen raw materials into powder by a pulverizing device, heating to room temperature, air-drying, sieving with 100 mesh sieve, and collecting the sieved material.
2. Preparing polypeptide raw materials: the pea protein powder is weighed and then mixed with pure water in a mass ratio of 1:15, and after being uniformly stirred by a stirring device, the temperature is controlled at 45 ℃ and soaked for 3 hours; weighing clean dry earthworm by using a weighing device, adding the dry earthworm into a crusher, adding 65% alcohol into the crusher, gradually adding alcohol into the crusher, and starting the crusher to crush and mix the dry earthworm after adding the dry earthworm;
3. pea peptide hydrolysis: cooling the mixed solution of pea protein and water to room temperature, hydrolyzing the mixed solution by adding protease a into the mixed solution, heating the mixed solution in the hydrolysis process, controlling the temperature to be 60 ℃, and continuously stirring by magnetic force;
4. pea peptide acquisition: after the enzymolysis of the pea protein is finished, heating the enzymolysis liquid a after the treatment to 90 ℃, carrying out enzyme deactivation treatment for 10-20 min, carrying out enzyme deactivation treatment on the protease a in the enzymolysis liquid a, carrying out centrifugal treatment on the enzymolysis liquid a by a centrifugal machine to obtain a supernatant a, and separating by a separation membrane a to obtain a pea peptide solution a;
5. obtaining earthworm natural polypeptide: after alcohol and earthworm are uniformly mixed, the mixture is sent to a flash extractor through a conveying pipeline, the mixture is extracted through the flash extractor for 1 minute, after the flash extraction is finished, the mixture is centrifuged through a low-speed centrifuge, the sediment in the mixed liquid after the flash extraction is separated from a supernatant b, and the supernatant b is separated through a separation membrane b, so that a natural earthworm polypeptide solution b and a earthworm protein solution c are obtained;
6. treatment of earthworm hydrolyzed polypeptides: adding distilled water into the earthworm protein solution c for mixing, wherein the mixing ratio is 1:2, placing the mixed solution into a constant-temperature ultrasonic extractor for ultrasonic extraction, then placing the mixed solution into the constant-temperature ultrasonic extractor for ultrasonic extraction, centrifuging the suspension after ultrasonic extraction at a high speed and a low temperature by a centrifuge, and taking a supernatant c after the low-temperature centrifugation is completed;
7. obtaining earthworm hydrolyzed polypeptide: adding distilled water into the supernatant c, which is 5 times of the supernatant in volume, adding protease b accounting for 2% of the mass of the earthworm, and magnetically stirring for 2 hours; after stirring, separating the enzymolysis liquid c by a separation membrane c immediately, separating protease and hydrolyzed polypeptide, centrifuging the separated enzymolysis liquid by a centrifuge by a low-speed centrifuge to obtain a supernatant d, and separating the supernatant d by the separation membrane d to obtain a earthworm hydrolyzed polypeptide solution d;
8. vacuum drying: respectively placing the pea peptide solution a, the earthworm natural polypeptide solution b and the earthworm hydrolyzed polypeptide solution d into a vacuum dryer for drying treatment, setting the temperature to 50 ℃, and taking out the solid after the moisture is completely dried to obtain pea peptide powder, earthworm natural polypeptide powder and earthworm hydrolyzed polypeptide powder;
9. the finished product is prepared: sequentially mixing the screened material obtained in the step 1, the pea peptide powder, the earthworm natural polypeptide powder, the earthworm hydrolyzed polypeptide powder, the chitosan, the concentrated whey protein powder and the protein powder into sugar powder according to mass, and pressing the sugar powder into slices by a press to obtain the health-care product slices.
Preferably, the obtained earthworm natural polypeptide solution b is separated by a separation membrane e to obtain a earthworm natural polypeptide solution c, and the earthworm natural polypeptide solution c is continued to be operated subsequently.
Preferably, the separation membrane a, the separation membrane d and the separation membrane e are nanofiltration membranes with molecular weight of 1000 daltons, and the separation membrane b and the separation membrane c are ultrafiltration membranes with molecular weight of 10000 daltons. The separation membrane a, the separation membrane d and the separation membrane e are set as nanofiltration membranes of 1000 daltons, so that small aggregated pea peptide, earthworm hydrolytic polypeptide and earthworm natural polypeptide can be obtained;
preferably, the proteinase a is thermophilic proteinase, the mixed solution is continuously heated in the hydrolysis process, the temperature is controlled to be 60-65 ℃, the proteinase b is papain, and dilute acid solution is continuously added in the stirring process to adjust the pH value, and the pH value is controlled to be 6.0.
Preferably, after the pea protein solution in the step 2 is mixed with water, adding a dilute alkali solution to adjust the pH value to 9.0, performing microwave extraction for 15min, controlling the temperature at 55 ℃, then preserving the heat for 60min to obtain a modified pea protein solution, and then performing subsequent flow through the modified pea protein solution.
Preferably, the dilute alkali solution is a weak alkaline solution with pH value of the feed liquid adjusted to 8.5-9.0 by 1.0mol/L NaOH solution, and the dilute acid solution with pH value adjusted to 5.5-6 by 1.0mol/L hydrochloric acid (HCl) solution.
Preferably, the condition temperature of the suspension after ultrasonic extraction in the step 6 is-4 ℃, the rotating speed is 11000r/min, and the time is 50min.
Compared with the related art, the health care product of the pea oligopeptide and the preparation method thereof have the following beneficial effects:
1. according to the invention, the earthworm peptide and the pea peptide are arranged in the raw materials, all essential amino acids of a human body can be provided in a balanced way through adding animal and plant double proteins, and the protein is replaced by the small-aggregation earthworm peptide and pea peptide, so that on one hand, the front and back effects are consistent, on the other hand, the earthworm peptide is more convenient to absorb, and the earthworm peptide is a mixture of natural polypeptide and hydrolytic polypeptide, so that the earthworm peptide has the characteristics of both the natural polypeptide and the hydrolytic polypeptide, and the effect of a product is improved.
2. The pea peptide with the blood pressure reducing function can be obtained by hydrolyzing pea protein with thermophilic proteinase, and the pea peptide is filtered by using a nanofiltration membrane with the molecular weight of 1000, so that the small-aggregate pea peptide can be obtained, has the effects of preventing and treating diseases and regulating the small-aggregate polypeptide of the physiological function of a human body, and is dried by a vacuum dryer, so that oxidation in the drying process is prevented, and the effect of a product is influenced;
3. the invention can simultaneously extract the natural earthworm polypeptide and the hydrolyzed earthworm polypeptide by the method, improves the utilization rate of earthworm raw materials, and can obtain the small-aggregate natural earthworm polypeptide by using the nanofiltration membrane with the molecular weight of 1000 for secondary filtration, so that the small-aggregate natural earthworm polypeptide has the effects of preventing and treating diseases and regulating physiological functions of human bodies, and the extraction efficiency is more efficient by using papain for extraction.
Drawings
FIG. 1 is a schematic flow chart of a method for preparing a pea oligopeptide health care product;
FIG. 2 is a flow chart of pea peptide preparation;
FIG. 3 is a flow chart of the preparation of natural polypeptide of Lumbricus;
FIG. 4 is a flow chart of the preparation of natural polypeptide of Lumbricus.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Specific implementations of the invention are described in detail below in connection with specific embodiments.
Example 1
A health product of pea oligopeptide comprises the following main components in percentage by mass: 40 parts of pea peptide powder, 10 parts of earthworm natural polypeptide powder, 30 parts of earthworm hydrolyzed polypeptide powder, 8 parts of bone meal, 5 parts of chitosan, 10 parts of concentrated whey protein powder, 6 parts of turtle shell, 1 part of ginseng powder and 0.5 part of calcium carbonate.
Example 2
The health-care product of pea oligopeptide comprises the main components of 80 parts of pea peptide powder, 20 parts of earthworm natural polypeptide powder, 60 parts of earthworm hydrolyzed polypeptide powder, 12 parts of bone meal, 7 parts of chitosan, 12 parts of concentrated whey protein powder, 8 parts of turtle shell, 2 parts of ginseng powder and 1 part of calcium carbonate according to the mass ratio.
Example 3
The health-care product of pea oligopeptide comprises the main components of 120 parts of pea peptide powder, 30 parts of earthworm natural polypeptide powder, 90 parts of earthworm hydrolyzed polypeptide powder, 13 parts of bone meal, 9 parts of chitosan, 14 parts of concentrated whey protein powder, 9 parts of turtle shell, 3 parts of ginseng powder and 1.5 parts of calcium carbonate according to the mass ratio.
The specific preparation method of the above examples 1-3 is
1. Crushing raw materials: sterilizing bone, soft-shelled turtle shell and ginseng, quick-freezing by a freezing device, pulverizing the quick-frozen raw materials into powder by a pulverizing device, heating to room temperature, air-drying, sieving with 100 mesh sieve, and collecting the sieved material.
2. Preparing polypeptide raw materials: the pea protein powder is weighed and then mixed with pure water in a mass ratio of 1:15, and after being uniformly stirred by a stirring device, the temperature is controlled at 45 ℃ and soaked for 3 hours; adding 1.0mol/L NaOH solution to adjust the pH value of alkali to 9.0, carrying out microwave extraction for 15min, controlling the temperature at 55 ℃, then preserving heat for 60min to obtain modified pea protein solution, taking clean dry earthworm, weighing the clean dry earthworm by using a weighing device, adding the dry earthworm into a crusher, adding 65% alcohol into the crusher, gradually adding the alcohol, starting the crusher to crush and mix the dry earthworm after adding the alcohol, wherein the ratio of the volume of the added alcohol to the mass value of the earthworm is 4L/1 kg;
3. pea peptide hydrolysis: placing and cooling the mixed solution of the modified pea protein solution and water to room temperature, adding thermolysin into the mixed solution to hydrolyze the mixed solution, heating the mixed solution in the hydrolysis process, controlling the temperature to be 60 ℃, and continuously stirring the mixed solution by magnetic force;
4. pea peptide acquisition: after the enzymolysis of the pea protein is finished, heating the enzymolysis liquid a after the treatment to 90 ℃, carrying out enzyme deactivation treatment for 10-20 min, carrying out enzyme deactivation treatment on thermophilic proteinase in the enzymolysis liquid a, carrying out centrifugal treatment on the enzymolysis liquid a by a centrifugal machine to obtain a supernatant a, and separating by a nanofiltration membrane with 1000 molecular weight to obtain a pea peptide solution a;
5. obtaining earthworm natural polypeptide: after alcohol and earthworm are uniformly mixed, the mixture is sent to a flash extractor through a conveying pipeline, the mixture is extracted through the flash extractor for 1 minute, after the flash extraction is finished, the mixture is centrifuged through a low-speed centrifuge, the sediment in the mixed liquid after the flash extraction is separated from a supernatant b, and the supernatant b is separated through an ultrafiltration membrane of 10000 daltons, so that a natural earthworm polypeptide solution b and a earthworm protein solution c are obtained;
6. treatment of earthworm hydrolyzed polypeptides: adding distilled water into the earthworm protein solution c for mixing, wherein the mixing ratio is 1:2, placing the mixed solution into a constant-temperature ultrasonic extractor for ultrasonic extraction, then placing the mixed solution into the constant-temperature ultrasonic extractor for ultrasonic extraction, and centrifuging the suspension after ultrasonic extraction at high speed and low temperature by a centrifuge at the temperature of-4 ℃ at the rotating speed of 11000r/min for 50min. Taking supernatant c after low-temperature centrifugation is completed;
7. obtaining earthworm hydrolyzed polypeptide: adding distilled water into the supernatant c, which is 5 times of the supernatant in volume, adding papain accounting for 2% of the mass of the earthworm, and magnetically stirring for 2 hours; separating the enzymolysis liquid c through an ultrafiltration membrane of 10000 daltons immediately after stirring, separating papain and hydrolyzed polypeptide, centrifuging the separated enzymolysis liquid through a centrifuge by using a low-speed centrifuge to obtain a supernatant d, and separating the supernatant d through a separation membrane d to obtain a earthworm hydrolyzed polypeptide solution d;
8. vacuum drying: respectively placing the pea peptide solution a, the earthworm natural polypeptide solution b and the earthworm hydrolyzed polypeptide solution d into a vacuum dryer for drying treatment, setting the temperature to 50 ℃, and taking out the solid after the moisture is completely dried to obtain pea peptide powder, earthworm natural polypeptide powder and earthworm hydrolyzed polypeptide powder;
9. the finished product is prepared: sequentially mixing the screened material obtained in the step 1, the pea peptide powder, the earthworm natural polypeptide powder, the earthworm hydrolyzed polypeptide powder, the chitosan, the concentrated whey protein powder and the protein powder into sugar powder according to mass, and pressing the sugar powder into slices by a press to obtain the health-care product slices.
The health care product of the pea oligopeptide provided by the invention has the following efficacy principle:
the method comprises the steps of arranging earthworm peptide and pea peptide in raw materials, uniformly providing all essential amino acids of a human body by adding animal and plant double proteins, replacing proteins with small aggregated earthworm peptide and pea peptide, enabling the front and rear effects to be consistent on one hand, enabling the small aggregated earthworm peptide and pea peptide to be more convenient to absorb on the other hand, enabling the small aggregated earthworm peptide to have the characteristics of natural polypeptide and hydrolyzed polypeptide simultaneously by selecting the mixture of the natural polypeptide and hydrolyzed polypeptide, improving the effects of products, hydrolyzing pea protein by thermophilic protease, obtaining pea peptide with a depressurization function, filtering by using a nano filter membrane with an interception 1000 molecular weight, obtaining small aggregated pea peptide, enabling the small aggregated pea peptide to have the effects of preventing and treating diseases, adjusting the small aggregated polypeptide of physiological functions of the human body, drying the small aggregated pea peptide by a vacuum dryer, preventing oxidation in the drying process, enabling the natural polypeptide to have the effects of preventing and treating diseases by using a nano filter membrane with a molecular weight of 1000 for secondary filtration, and enabling the small aggregated earthworm polypeptide to have the effects of preventing and treating diseases by using the nano filter membrane with a molecular weight of 1000.
The circuits and control involved in the present invention are all of the prior art, and are not described in detail herein.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present invention.
Claims (9)
1. The health care product of pea oligopeptide is characterized by comprising the following main components in percentage by mass: 40-120 parts of pea peptide powder, 40-120 parts of earthworm peptide powder, 8-12 parts of bone powder, 5-10 parts of chitosan, 10-15 parts of concentrated whey protein powder, 6-9 parts of turtle shell, 1-3 parts of ginseng powder and 0.5-1.5 parts of calcium carbonate.
2. The health product of pea oligopeptide according to claim 1, wherein the earthworm peptide powder comprises 10-30 parts of earthworm natural polypeptide powder and 30-90 parts of earthworm hydrolyzed polypeptide powder, and the pea peptide powder is pea peptide with molecular weight below 3000 daltons.
3. The preparation method of the pea oligopeptide health-care product is characterized by comprising the following specific steps of:
(1) Crushing raw materials: sterilizing bone, soft-shelled turtle shell and ginseng, quick-freezing by a freezing device, pulverizing the quick-frozen raw materials into powder by a pulverizing device, heating to room temperature, air-drying, sieving with 100 mesh sieve, and collecting the sieved material.
(2) Preparing polypeptide raw materials: the pea protein powder is weighed and then mixed with pure water in a mass ratio of 1:15, and after being uniformly stirred by a stirring device, the temperature is controlled at 45 ℃ and soaked for 3 hours; weighing clean dry earthworm by using a weighing device, adding the dry earthworm into a crusher, adding 65% alcohol into the crusher, gradually adding alcohol into the crusher, and starting the crusher to crush and mix the dry earthworm after adding the dry earthworm;
(3) Pea peptide hydrolysis: cooling the mixed solution of pea protein and water to room temperature, hydrolyzing the mixed solution by adding protease a into the mixed solution, heating the mixed solution in the hydrolysis process, controlling the temperature to be 60 ℃, and continuously stirring by magnetic force;
(4) Pea peptide acquisition: after the enzymolysis of the pea protein is finished, heating the enzymolysis liquid a after the treatment to 90 ℃, carrying out enzyme deactivation treatment for 10-20 min, carrying out enzyme deactivation treatment on the protease a in the enzymolysis liquid a, carrying out centrifugal treatment on the enzymolysis liquid a by a centrifugal machine to obtain a supernatant a, and separating by a separation membrane a to obtain a pea peptide solution a;
(5) Obtaining earthworm natural polypeptide: after alcohol and earthworm are uniformly mixed, the mixture is sent to a flash extractor through a conveying pipeline, the mixture is extracted through the flash extractor for 1 minute, after the flash extraction is finished, the mixture is centrifuged through a low-speed centrifuge, the sediment in the mixed liquid after the flash extraction is separated from a supernatant b, and the supernatant b is separated through a separation membrane b, so that a natural earthworm polypeptide solution b and a earthworm protein solution c are obtained;
(6) Treatment of earthworm hydrolyzed polypeptides: adding distilled water into the earthworm protein solution c for mixing, wherein the mixing ratio is 1:2, placing the mixed solution into a constant-temperature ultrasonic extractor for ultrasonic extraction, then placing the mixed solution into the constant-temperature ultrasonic extractor for ultrasonic extraction, centrifuging the suspension after ultrasonic extraction at a high speed and a low temperature by a centrifuge, and taking a supernatant c after the low-temperature centrifugation is completed;
(7) Obtaining earthworm hydrolyzed polypeptide: adding distilled water into the supernatant c, which is 5 times of the supernatant in volume, adding protease b accounting for 2% of the mass of the earthworm, and magnetically stirring for 2 hours; after stirring, separating the enzymolysis liquid c by a separation membrane c immediately, separating protease and hydrolyzed polypeptide, centrifuging the separated enzymolysis liquid by a centrifuge by a low-speed centrifuge to obtain a supernatant d, and separating the supernatant d by the separation membrane d to obtain a earthworm hydrolyzed polypeptide solution d;
(8) Vacuum drying: respectively placing the pea peptide solution a, the earthworm natural polypeptide solution b and the earthworm hydrolyzed polypeptide solution d into a vacuum dryer for drying treatment, setting the temperature to 50 ℃, and taking out the solid after the moisture is completely dried to obtain pea peptide powder, earthworm natural polypeptide powder and earthworm hydrolyzed polypeptide powder;
(9) The finished product is prepared: sequentially mixing the screened material obtained in the step 1, the pea peptide powder, the earthworm natural polypeptide powder, the earthworm hydrolyzed polypeptide powder, the chitosan, the concentrated whey protein powder and the protein powder into sugar powder according to mass, and pressing the sugar powder into slices by a press to obtain the health-care product slices.
4. The method for preparing a health product of pea oligopeptide according to claim 3, wherein the obtained natural polypeptide solution b is separated by a separation membrane e to obtain a natural polypeptide solution c, and the natural polypeptide solution c is further processed.
5. The method for preparing the pea oligopeptide health care product according to claim 3, wherein the separation membrane a, the separation membrane d and the separation membrane e are nanofiltration membranes with molecular weight of 1000 daltons, and the separation membrane b and the separation membrane c are ultrafiltration membranes with molecular weight of 10000 daltons.
6. The method for preparing a health product of pea oligopeptide according to claim 3, wherein the protease a is thermolysin, the mixed solution is heated continuously during the hydrolysis process, the temperature is controlled to be 60-65 ℃, the protease b is papain, and dilute acid solution is added continuously during the stirring process to adjust the pH value to 6.0.
7. The method for preparing a health product of pea oligopeptide according to claim 6, wherein the pea protein solution in the step (2) is mixed with water, diluted alkaline solution is added to adjust the pH value to 9.0, the mixture is subjected to microwave extraction for 15min, the temperature is controlled at 55 ℃, the mixture is then kept for 60min, and a modified pea protein solution is obtained, and then the subsequent process is carried out through the modified pea protein solution.
8. The method for preparing a health product of pea oligopeptide according to claim 7, wherein the dilute alkali solution is a weak alkaline solution with a pH value of 8.5-9.0 adjusted by 1.0mol/L NaOH solution, and the dilute acid solution with a pH value of 5.5-6 adjusted by 1.0mol/L hydrochloric acid (HCl) solution.
9. The method for preparing a pea oligopeptide health care product according to claim 3, wherein the condition of centrifugation of the suspension after ultrasonic extraction in the step (6) is-4 ℃, the rotating speed is 11000r/min, and the time is 50min.
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